Pub Date : 2022-06-30DOI: 10.33736/bjrst.4224.2022
Chyntia Jaby ANAK ENTUNI, T. Zulcaffle
Corn is a vital commodity in Malaysia because it is a key component of animal feed. The retention of the wholesome corn yield is essential to satisfy the rising demand. Like other plants, corn is susceptible to pathogens infection during the growing period. Manual observation of the diseases nevertheless takes time and requires a lot of work. The aim of this study was to propose an automatic approach to identify corn leaf diseases. The dataset used comprises of the images of diseased corn leaf comprising of blight, grey spot and rust as well as healthy corn leaf in YCbCr colour space representation. The DenseNet-201 algorithm was utilised in the proposed method of identifying corn leaf diseases. The training and validation analysis of distinctive epoch values of DenseNet-201 were also used to validate the proposed method, which resulted in significantly higher identification accuracy. DenseNet-201 succeeded 95.11% identification accuracy and it outperformed the prior identification methods such as ResNet-50, ResNet-101 and Bag of Features. The DenseNet-201 also has been validated to function as anticipated in identifying corn leaf diseases based on the algorithm validation assessment.
玉米是马来西亚的重要商品，因为它是动物饲料的关键成分。保持健康的玉米产量对于满足不断增长的需求至关重要。与其他植物一样，玉米在生长期间易受病原体感染。然而，人工观察疾病需要时间，需要大量工作。本研究的目的是提出一种自动识别玉米叶片病害的方法。所使用的数据集包括YCbCr颜色空间表示中的患病玉米叶（包括枯萎病、灰斑和铁锈）以及健康玉米叶的图像。DenseNet-201算法被用于所提出的玉米叶片病害识别方法。DenseNet-201的不同历元值的训练和验证分析也用于验证所提出的方法，从而显著提高了识别精度。DenseNet-201的识别准确率为95.11%，优于现有的识别方法，如ResNet-50、ResNet-101和Bag of Features。DenseNet-201也已根据算法验证评估，在识别玉米叶片疾病方面发挥了预期的作用。
{"title":"Identification of Corn Leaf Diseases Comprising of Blight, Grey Spot and Rust Using DenseNet-201","authors":"Chyntia Jaby ANAK ENTUNI, T. Zulcaffle","doi":"10.33736/bjrst.4224.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4224.2022","url":null,"abstract":"Corn is a vital commodity in Malaysia because it is a key component of animal feed. The retention of the wholesome corn yield is essential to satisfy the rising demand. Like other plants, corn is susceptible to pathogens infection during the growing period. Manual observation of the diseases nevertheless takes time and requires a lot of work. The aim of this study was to propose an automatic approach to identify corn leaf diseases. The dataset used comprises of the images of diseased corn leaf comprising of blight, grey spot and rust as well as healthy corn leaf in YCbCr colour space representation. The DenseNet-201 algorithm was utilised in the proposed method of identifying corn leaf diseases. The training and validation analysis of distinctive epoch values of DenseNet-201 were also used to validate the proposed method, which resulted in significantly higher identification accuracy. DenseNet-201 succeeded 95.11% identification accuracy and it outperformed the prior identification methods such as ResNet-50, ResNet-101 and Bag of Features. The DenseNet-201 also has been validated to function as anticipated in identifying corn leaf diseases based on the algorithm validation assessment.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44145256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4215.2022
Muhamad Kabiru Abubakar, Amir Shah Ruddin Md Sah, Alyaa Filza Effendi
The distribution of the phytoplankton community in different locations of Puah Reservoir, Malaysia was investigated from May to December 2019 to examine the phytoplankton compositions and density. Seven sampling locations were selected namely Terengganu Mati (P1), Limbing Besar (P2), Temba Outlet (P3), Pela-gong (P4), Sireh (P5), Centre Dam (P6) and Power Intake (P7). A total of 543 cell/mL were recorded from 35 genera in the reservoir. These identified species belonged to six major plankton groups: 12 green algae (35.29%), nine blue-green algae (26.47%), nine diatom (23.53%), two golden algae (5.88%), two filamentous (5.88%) and one flagellate (2.94%). During this period, Chlorophyta was the most abundant group (40% of the total phytoplankton), followed by Bacillariophyceae (29%), Pyrrhophyta (19%), Cyanophyta (12%) and Chrysophyceae (1%). The highest composition of phytoplankton was recorded at P7 (32%), followed by P3 (16%), P1 (14%) and P4 and P2 with the lowest (8% and 7%), respectively. The lowest density was observed during dry season (162 cell/mL) and high density during wet season (412 cell/mL). Species richness was discreetly greater in the wet season however, evenness index was ≥0.8, thereby indicating a similarity in species abundance. The water temperature, pH and dissolved oxygen correlate positively with phytoplankton at P = 0.01. The overall mean values of temperature for wet and dry season were 29.3 ± 1.79 °C and 27.5 ± 1.55 °C while dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD) and total suspended solid (TSS) were 16.2 mg/L and 4.7 mg/L, 3.4 mg/L and 2.9 mg/L, 15.2 mg/L and 12.7 mg/L, and 0.5 mg/L and 2.3 mg/L, respectively. Water transparency, pH and DO were found to be important factors characterising each sampling location.
{"title":"The Inter-Seasonal Phytoplankton Compositions and Density in Response to Abiotic Factors in Puah Reservoir Hulu Terengganu Hydroelectric Dam, Peninsular Malaysia","authors":"Muhamad Kabiru Abubakar, Amir Shah Ruddin Md Sah, Alyaa Filza Effendi","doi":"10.33736/bjrst.4215.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4215.2022","url":null,"abstract":"The distribution of the phytoplankton community in different locations of Puah Reservoir, Malaysia was investigated from May to December 2019 to examine the phytoplankton compositions and density. Seven sampling locations were selected namely Terengganu Mati (P1), Limbing Besar (P2), Temba Outlet (P3), Pela-gong (P4), Sireh (P5), Centre Dam (P6) and Power Intake (P7). A total of 543 cell/mL were recorded from 35 genera in the reservoir. These identified species belonged to six major plankton groups: 12 green algae (35.29%), nine blue-green algae (26.47%), nine diatom (23.53%), two golden algae (5.88%), two filamentous (5.88%) and one flagellate (2.94%). During this period, Chlorophyta was the most abundant group (40% of the total phytoplankton), followed by Bacillariophyceae (29%), Pyrrhophyta (19%), Cyanophyta (12%) and Chrysophyceae (1%). The highest composition of phytoplankton was recorded at P7 (32%), followed by P3 (16%), P1 (14%) and P4 and P2 with the lowest (8% and 7%), respectively. The lowest density was observed during dry season (162 cell/mL) and high density during wet season (412 cell/mL). Species richness was discreetly greater in the wet season however, evenness index was ≥0.8, thereby indicating a similarity in species abundance. The water temperature, pH and dissolved oxygen correlate positively with phytoplankton at P = 0.01. The overall mean values of temperature for wet and dry season were 29.3 ± 1.79 °C and 27.5 ± 1.55 °C while dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD) and total suspended solid (TSS) were 16.2 mg/L and 4.7 mg/L, 3.4 mg/L and 2.9 mg/L, 15.2 mg/L and 12.7 mg/L, and 0.5 mg/L and 2.3 mg/L, respectively. Water transparency, pH and DO were found to be important factors characterising each sampling location.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47018791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.3819.2022
A. Tuen, Lydia Poi Huong Toh, Ervynna Christy Bandong
The poultry industry is under pressure from the rising cost of imported feed ingredients. In order for the industry to be profitable and sustainable, it has to reduce the cost of production by using locally available feedstuff. A study was carried out to evaluate feed preference of broiler chicken for cassava root chip, for three diets made by mixing different proportion in percentage of cassava root chip and ground Moringa leaf (90:10, 80:20, 70:30) and compare it to the preference for commercial starter diet. These diets were offered as free choice to five 20-days old chicken, one at a time over a period of five days. The three feed preference criteria assessed were (i) the visiting frequency to each diet station, (ii) time spent feed at each diet station, and (iii) amount of different treatment diet consumed. The hypothesis is that the preferred food will be visited more often; the chicken will spend longer time feeding on it and therefore consume it more. We found that broiler chicken consumed cassava root chip the least (2 g), visited it less often (9 visits) and spent less time (3.5 minutes) feeding on it. In contrast, feed consumption (6.12 g) and time spent (17 minutes) was highest for mixed diet containing 80% cassava and 20% Moringa leaf meal. Visiting frequency was highest for commercial starter diet (29 visits) and second highest (24 visits) for mixed diet of 80% cassava: 20% Moringa and 70% cassava: 30% Moringa. It is concluded that broiler chicken preferred the diet containing 80% cassava root chip and 20% Moringa leaf meal among all diets on offer.
{"title":"Preference of Broiler Chicken for Cassava-Moringa Based Diet","authors":"A. Tuen, Lydia Poi Huong Toh, Ervynna Christy Bandong","doi":"10.33736/bjrst.3819.2022","DOIUrl":"https://doi.org/10.33736/bjrst.3819.2022","url":null,"abstract":"The poultry industry is under pressure from the rising cost of imported feed ingredients. In order for the industry to be profitable and sustainable, it has to reduce the cost of production by using locally available feedstuff. A study was carried out to evaluate feed preference of broiler chicken for cassava root chip, for three diets made by mixing different proportion in percentage of cassava root chip and ground Moringa leaf (90:10, 80:20, 70:30) and compare it to the preference for commercial starter diet. These diets were offered as free choice to five 20-days old chicken, one at a time over a period of five days. The three feed preference criteria assessed were (i) the visiting frequency to each diet station, (ii) time spent feed at each diet station, and (iii) amount of different treatment diet consumed. The hypothesis is that the preferred food will be visited more often; the chicken will spend longer time feeding on it and therefore consume it more. We found that broiler chicken consumed cassava root chip the least (2 g), visited it less often (9 visits) and spent less time (3.5 minutes) feeding on it. In contrast, feed consumption (6.12 g) and time spent (17 minutes) was highest for mixed diet containing 80% cassava and 20% Moringa leaf meal. Visiting frequency was highest for commercial starter diet (29 visits) and second highest (24 visits) for mixed diet of 80% cassava: 20% Moringa and 70% cassava: 30% Moringa. It is concluded that broiler chicken preferred the diet containing 80% cassava root chip and 20% Moringa leaf meal among all diets on offer.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49528892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4495.2022
J. Nelson, G. Noweg, I. Jusoh
Over the years, the co-inoculation of Trichoderma and arbuscular mycorrhizal fungi (AMF) with different types of fertiliser in a field condition has been understudied. This study explores the potential of Trichoderma and AMF mixture (T-AMF) with different types of fertiliser for plant growth with the objectives to: (i) analyse the growth of Durio zibethinus and Artocarpus heterophyllus fertilised with different types of fertiliser and inoculated with T-AMF and (ii) determine the optimal combination of organic and chemical fertiliser with T-AMF. A randomized complete block design was applied using seven treatments namely, 50 g organic fertiliser (OF) + T-AMF, 100 g OF + T-AMF, 50 g chemical fertiliser (CF) + T-AMF, 100 g CF + T-AMF, 100 g OF, 100 g CF, and control (without fertiliser and T-AMF). The growth analysis of D. zibethinus showed the highest mean height was at 100 g OF with T-AMF, stem diameter at 100 g CF with T-AMF, and the number of leaves at 50 g CF with T-AMF. For A. heterophyllus, the highest mean height and stem diameter was at 50 g OF with T-AMF and the number of leaves at 100 g OF with T-AMF. The optimal combination of fertiliser with T-AMF for D. zibethinus’ height was 100 g OF and number of leaves was 50 g CF. The optimal combination of fertiliser with T-AMF for A. heterophyllus’ height and stem diameter was 50 g OF. The optimal combination for its number of leaves was 100 g OF with T-AMF. This concludes that the application of fertiliser with T-AMF reacted differently to plant species and their growth parameters. The co-inoculation of Trichoderma and AMF may present a cheaper and sustainable alternative, especially when the planting scale is huge.
多年来，人们对木霉和丛枝菌根真菌(AMF)与不同类型肥料在田间条件下的共接种进行了深入研究。本研究探讨木霉与AMF混合物(T-AMF)与不同类型肥料对植物生长的潜力，目的是:(i)分析不同类型肥料和接种T-AMF的紫豆(Durio zibethinus)和异叶鹿角(Artocarpus heterophyllus)的生长情况;(ii)确定有机和化学肥料与T-AMF的最佳组合。采用随机完全区组设计，采用有机肥+ T-AMF 50 g、有机肥+ T-AMF 100 g、化肥+ T-AMF 50 g、有机肥+ T-AMF 100 g、有机肥100 g、有机肥100 g、有机肥100 g和对照(不施用有机肥和T-AMF) 7个处理。生长分析结果表明，T-AMF处理后的平均株高在100 g时最高，茎粗在100 g CF时最高，叶数在50 g CF时最高。在50 g OF T-AMF处理下，杂叶草的平均株高和茎粗最高，叶片数在100 g OF T-AMF处理下最高。施肥与T-AMF的最优配施量为100 g of of，叶片数为50 g CF;施肥与T-AMF的最优配施量为50 g of of。其叶片数的最佳组合为100 g T-AMF。由此可见，施用T-AMF对植物种类及其生长参数有不同的影响。特别是在种植规模较大的情况下，木霉和AMF的共接种可能是一种经济、可持续的替代方法。
{"title":"Potential of Trichoderma and AMF Mixture with Different Types of Fertiliser for Durio zibethinus Murray (Durian) and Artocarpus heterophyllus Lam. (Jackfruit) Growth","authors":"J. Nelson, G. Noweg, I. Jusoh","doi":"10.33736/bjrst.4495.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4495.2022","url":null,"abstract":"Over the years, the co-inoculation of Trichoderma and arbuscular mycorrhizal fungi (AMF) with different types of fertiliser in a field condition has been understudied. This study explores the potential of Trichoderma and AMF mixture (T-AMF) with different types of fertiliser for plant growth with the objectives to: (i) analyse the growth of Durio zibethinus and Artocarpus heterophyllus fertilised with different types of fertiliser and inoculated with T-AMF and (ii) determine the optimal combination of organic and chemical fertiliser with T-AMF. A randomized complete block design was applied using seven treatments namely, 50 g organic fertiliser (OF) + T-AMF, 100 g OF + T-AMF, 50 g chemical fertiliser (CF) + T-AMF, 100 g CF + T-AMF, 100 g OF, 100 g CF, and control (without fertiliser and T-AMF). The growth analysis of D. zibethinus showed the highest mean height was at 100 g OF with T-AMF, stem diameter at 100 g CF with T-AMF, and the number of leaves at 50 g CF with T-AMF. For A. heterophyllus, the highest mean height and stem diameter was at 50 g OF with T-AMF and the number of leaves at 100 g OF with T-AMF. The optimal combination of fertiliser with T-AMF for D. zibethinus’ height was 100 g OF and number of leaves was 50 g CF. The optimal combination of fertiliser with T-AMF for A. heterophyllus’ height and stem diameter was 50 g OF. The optimal combination for its number of leaves was 100 g OF with T-AMF. This concludes that the application of fertiliser with T-AMF reacted differently to plant species and their growth parameters. The co-inoculation of Trichoderma and AMF may present a cheaper and sustainable alternative, especially when the planting scale is huge.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46154724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4176.2022
S. Sazali
The notorious palm weevils from the genus Rhynchophorus (Coleoptera: Curculionidae) are known to be polymorphic and show high phenotypic plasticity. Due to these characteristics, this study attempts to document the typologies based on the pronotal markings observed from Kuala Terengganu population. Samples were collected using baited traps with pheromones and fruits as well as through handpicking method, where the sampling sites were located at two villages, namely, Kampung Tanjung Paya and Kampung Pulau Sekati. A total of 100 individuals were collected of which 19 typologies were successfully identified. Of all, the most prominent pattern observed was typology A, represented by 37 individuals. Findings from this study suggested that the variation was driven by various factors including resource limitation, habitat preference, diet preference and competition. However, a comprehensive study should be initiated to measure possible factor(s) which possibly induce the pronotal variation within Rhynchophorus at a local scale.
众所周知，Rhynchophorus属（鞘翅目：弯甲科）臭名昭著的棕榈象甲具有多态性，并表现出高度的表型可塑性。由于这些特征，本研究试图根据从Kuala Terengganu人群中观察到的前标记来记录类型学。使用带有信息素和水果的诱饵陷阱以及手工挑选方法收集样本，采样点位于两个村庄，即Kampung Tanjung Paya和Kampung Pulau Sekati。共收集了100个个体，其中19个类型被成功识别。在所有类型中，观察到的最突出的模式是类型A，由37个个体代表。这项研究的结果表明，这种变化是由各种因素驱动的，包括资源限制、栖息地偏好、饮食偏好和竞争。然而，应该开始一项全面的研究，以测量可能在局部范围内诱导Rhynchophorus内的前体变异的可能因素。
{"title":"Variation of the Pronotal Markings in Rhynchophorus (Coleoptera: Curculionidae) Species from Kuala Terengganu, Terengganu","authors":"S. Sazali","doi":"10.33736/bjrst.4176.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4176.2022","url":null,"abstract":"The notorious palm weevils from the genus Rhynchophorus (Coleoptera: Curculionidae) are known to be polymorphic and show high phenotypic plasticity. Due to these characteristics, this study attempts to document the typologies based on the pronotal markings observed from Kuala Terengganu population. Samples were collected using baited traps with pheromones and fruits as well as through handpicking method, where the sampling sites were located at two villages, namely, Kampung Tanjung Paya and Kampung Pulau Sekati. A total of 100 individuals were collected of which 19 typologies were successfully identified. Of all, the most prominent pattern observed was typology A, represented by 37 individuals. Findings from this study suggested that the variation was driven by various factors including resource limitation, habitat preference, diet preference and competition. However, a comprehensive study should be initiated to measure possible factor(s) which possibly induce the pronotal variation within Rhynchophorus at a local scale.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42957132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4235.2022
Emy Ritta Jinggong, F. Khan
Karst landscape is recognised as one of the biologically important areas especially for bats. The limestone caves and forest within the landscape provide essential resources for the local bat fauna, albeit bat populations are severely threatened by anthropogenic disturbances occurring within and surrounding the ecosystem. In Sarawak, studies on bat diversity are biased towards protected areas, leaving aside non-protected areas including private lands such as Mount Silabur. Surveys on bat diversity were conducted at the limestone outcrop and the surrounding matrix of Mount Silabur with the primary aim of developing the first checklist of bats at Mount Silabur. A total of 27 species were recorded from 12 sampling nights representing approximately 34% of the total bat species recorded in Sarawak. The Cox’s Roundleaf Bat (Hipposideros coxi), a rare Bornean endemic species, was recorded from the cave system, signifying an important record for the area and also Sarawak. Furthermore, this is the sixth locality record for the species in Sarawak, Borneo. Interestingly, the total number of bat species recorded from Mount Silabur represented about 72% of the total bat species recorded (27 out of 38 species) from other major touristic caves in western Sarawak; Fairy Cave Nature Reserve and Wind Cave Nature Reserve. This warrants a need to develop an action plan to protect and conserve Mount Silabur cave system and the surrounding forest for its potential to serve as a shelter for bats and other cave and forest dependent taxa. The local community should implement and adhere to guidelines for cave related activities to minimise human driven disturbances on the ecosystem and its inhabitants.
{"title":"Bats of Mount Silabur, Sarawak, and Its Potential for Conservation","authors":"Emy Ritta Jinggong, F. Khan","doi":"10.33736/bjrst.4235.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4235.2022","url":null,"abstract":"Karst landscape is recognised as one of the biologically important areas especially for bats. The limestone caves and forest within the landscape provide essential resources for the local bat fauna, albeit bat populations are severely threatened by anthropogenic disturbances occurring within and surrounding the ecosystem. In Sarawak, studies on bat diversity are biased towards protected areas, leaving aside non-protected areas including private lands such as Mount Silabur. Surveys on bat diversity were conducted at the limestone outcrop and the surrounding matrix of Mount Silabur with the primary aim of developing the first checklist of bats at Mount Silabur. A total of 27 species were recorded from 12 sampling nights representing approximately 34% of the total bat species recorded in Sarawak. The Cox’s Roundleaf Bat (Hipposideros coxi), a rare Bornean endemic species, was recorded from the cave system, signifying an important record for the area and also Sarawak. Furthermore, this is the sixth locality record for the species in Sarawak, Borneo. Interestingly, the total number of bat species recorded from Mount Silabur represented about 72% of the total bat species recorded (27 out of 38 species) from other major touristic caves in western Sarawak; Fairy Cave Nature Reserve and Wind Cave Nature Reserve. This warrants a need to develop an action plan to protect and conserve Mount Silabur cave system and the surrounding forest for its potential to serve as a shelter for bats and other cave and forest dependent taxa. The local community should implement and adhere to guidelines for cave related activities to minimise human driven disturbances on the ecosystem and its inhabitants.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42293441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4140.2022
Yin Sing Ong, S. Teng
Freshwater macrophytes exist in varied life forms for example, emergent, submerged, floating-leaved and free-floating, and some of them have a heterophylly or multiple forms. The intraspecific variation in terms of morphology is common phenomena in macrophytes population; however, specific studies on morphology of freshwater macrophytes are still insufficient, especially in Pontian, Johor. Hence, this study aimed to characterise the freshwater macrophytes based on morphometrics and descriptive characteristics as well as to document their diversity and population. Thus, the morphometric measurements were done, the descriptive characteristics were documented in scientific photographs, the population of freshwater macrophytes was also estimated by using quadrat estimation technique. Additionally, the morphometrics of some collected samples were compared with the same sample in other studies to show the variation in the range of different measurements, the descriptive characteristics were also described as well as the population estimation assessed by area coverage was analysed in the form of statistical chart. The finding in this study showed variation in morphometric data and no intraspecific variation on phenotype of freshwater macrophytes in Pontian, Johor’s water.
{"title":"Morphological Characterisation and Documentation of Freshwater Macrophytes in Pontian, Johor's Water","authors":"Yin Sing Ong, S. Teng","doi":"10.33736/bjrst.4140.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4140.2022","url":null,"abstract":"Freshwater macrophytes exist in varied life forms for example, emergent, submerged, floating-leaved and free-floating, and some of them have a heterophylly or multiple forms. The intraspecific variation in terms of morphology is common phenomena in macrophytes population; however, specific studies on morphology of freshwater macrophytes are still insufficient, especially in Pontian, Johor. Hence, this study aimed to characterise the freshwater macrophytes based on morphometrics and descriptive characteristics as well as to document their diversity and population. Thus, the morphometric measurements were done, the descriptive characteristics were documented in scientific photographs, the population of freshwater macrophytes was also estimated by using quadrat estimation technique. Additionally, the morphometrics of some collected samples were compared with the same sample in other studies to show the variation in the range of different measurements, the descriptive characteristics were also described as well as the population estimation assessed by area coverage was analysed in the form of statistical chart. The finding in this study showed variation in morphometric data and no intraspecific variation on phenotype of freshwater macrophytes in Pontian, Johor’s water.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45403769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4529.2022
Nur Syafiqah Mashhor, S. F. Z. Mohamad Fuzi, N. Abdullah, S. Asman
Durian (Durio zibethinus) is a popular seasonal fruit in Southeast Asia. Pectin can be extracted from the rind. Pectin is an excellent source of fibre and is available in two forms: high and low methoxyl pectin. Both of these types of substances can be employed as gelling agents. As such, the purpose of this research is to partially characterise durian pectin in order to facilitate the development of a pectin-based edible ink formulation. Four formulations of pectin-based food ink were developed and evaluated using a rheometer to determine the viscoelastic properties, a Fourier transform infrared (FTIR) to determine the chemical functional groups available, and thermogravimetric analysis to determine the thermal stability using durian rind waste pectin and commercial pectin. The results indicated that durian pectin contains a low amount of methoxyl (LM) at 2.48 ± 0.31%, which is appropriate for the development of food ink, whereas commercial pectin has a high methoxyl (HM) content of 28.72 ± 0.47%. With viscosities of 31759.20 Pa/s and 7482.62 Pa/s, formulations 3 and 4 of LM pectin exhibited the highest viscoelastic properties. The third and fourth formulations of HM pectin, as well as the third and fourth formulations of LM pectin, contain components that include the alcohol, carbonyl compound, and carbonyl group are found in both pectin granules. The LM pectin formulation 2 offers the highest thermal stability (32.00% residual weight) and the lowest weight loss percentage (57.15%). Due to its capacity to form gel, the LM result demonstrated potential for use in the formulation of edible ink. Additionally, it can be used as a polymeric crosslinker in conjunction with other materials.
{"title":"The Development of a Pectin-Based Food Ink from Locally Sourced Durian Rind Waste for Possible Use as a 3D Printable Food Material","authors":"Nur Syafiqah Mashhor, S. F. Z. Mohamad Fuzi, N. Abdullah, S. Asman","doi":"10.33736/bjrst.4529.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4529.2022","url":null,"abstract":"Durian (Durio zibethinus) is a popular seasonal fruit in Southeast Asia. Pectin can be extracted from the rind. Pectin is an excellent source of fibre and is available in two forms: high and low methoxyl pectin. Both of these types of substances can be employed as gelling agents. As such, the purpose of this research is to partially characterise durian pectin in order to facilitate the development of a pectin-based edible ink formulation. Four formulations of pectin-based food ink were developed and evaluated using a rheometer to determine the viscoelastic properties, a Fourier transform infrared (FTIR) to determine the chemical functional groups available, and thermogravimetric analysis to determine the thermal stability using durian rind waste pectin and commercial pectin. The results indicated that durian pectin contains a low amount of methoxyl (LM) at 2.48 ± 0.31%, which is appropriate for the development of food ink, whereas commercial pectin has a high methoxyl (HM) content of 28.72 ± 0.47%. With viscosities of 31759.20 Pa/s and 7482.62 Pa/s, formulations 3 and 4 of LM pectin exhibited the highest viscoelastic properties. The third and fourth formulations of HM pectin, as well as the third and fourth formulations of LM pectin, contain components that include the alcohol, carbonyl compound, and carbonyl group are found in both pectin granules. The LM pectin formulation 2 offers the highest thermal stability (32.00% residual weight) and the lowest weight loss percentage (57.15%). Due to its capacity to form gel, the LM result demonstrated potential for use in the formulation of edible ink. Additionally, it can be used as a polymeric crosslinker in conjunction with other materials.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45070339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4261.2022
M. A. Mohamad Arif, Faris Danish Yusaini
The self-assembly of a series of bisthiourea containing amino acid side chains has been studied in a large range of organic solvents. Self-assembly is driven mainly by hydrogen bonding groups of thiourea and amino acids moieties. Of all the synthesized compounds, only bisthiourea with alanine side chains, 3.5 formed thermoreversible gel in 50:3 dichloromethane:water mixture at minimum gel concentration of 0.5%. SEM micrographs of the gel showed the formation of entangled cross-linked fibres. The addition of anions such as Cl¯, F¯ and AcO¯ disrupted the gel network of 3.5 thus inducing the gel-sol transition. To investigate the ability of the bisthiourea to form metallogel, metal ions such as Co2+, Cu2+, Ni2+ and Pb2+ were introduced, however, none of them induce the formation of metallogel. In particular, the gels show good performance in the absorption of bromocresol green and Eriochrome black-T at 75% and 61% efficiency, respectively within 24 hours. The good dye absorption properties of gel 3.5 render the potential of bisthiourea gels as new dye absorption materials, which show significant benefit for water pollution treatment.
{"title":"Multi-Stimuli-Responsive Organogel Based on Bisthiourea Compounds for The Removal of Selected Organic Dyes","authors":"M. A. Mohamad Arif, Faris Danish Yusaini","doi":"10.33736/bjrst.4261.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4261.2022","url":null,"abstract":"The self-assembly of a series of bisthiourea containing amino acid side chains has been studied in a large range of organic solvents. Self-assembly is driven mainly by hydrogen bonding groups of thiourea and amino acids moieties. Of all the synthesized compounds, only bisthiourea with alanine side chains, 3.5 formed thermoreversible gel in 50:3 dichloromethane:water mixture at minimum gel concentration of 0.5%. SEM micrographs of the gel showed the formation of entangled cross-linked fibres. The addition of anions such as Cl¯, F¯ and AcO¯ disrupted the gel network of 3.5 thus inducing the gel-sol transition. To investigate the ability of the bisthiourea to form metallogel, metal ions such as Co2+, Cu2+, Ni2+ and Pb2+ were introduced, however, none of them induce the formation of metallogel. In particular, the gels show good performance in the absorption of bromocresol green and Eriochrome black-T at 75% and 61% efficiency, respectively within 24 hours. The good dye absorption properties of gel 3.5 render the potential of bisthiourea gels as new dye absorption materials, which show significant benefit for water pollution treatment. ","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43511590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.33736/bjrst.4358.2022
Y. Kwan, Joseph Wilson
Black pepper (Piper nigrum L.) is one of the most widely used spices in food, beverage, cosmetics, and medicine. Black pepper production has suffered from various fungal diseases. Microbial biological control is an essential part of integrated disease management to reduce the heavy reliance on chemical fungicides. Trichoderma fungi comprise a large group of rhizocompetent filamentous fungi widely used in the biocontrol of plant pathogens. Three field surveys conducted on five black pepper farms in Belaga, Sarawak, identified three fungal diseases: yellowing, black berry, and foot rot. Based on the morphological and molecular characterisation, the identified fungal causal agents were Fusarium solani (yellowing disease), Colletotrichum gloeosporioides (black berry disease), and Phytophthora palmivora (foot rot disease). Twenty isolates of Trichoderma spp. were isolated from secondary forest and Biopark in Bintulu, Sarawak. Trichoderma isolates were characterised based on the morphological characteristics and molecular phylogenetic analysis using the rDNA internal transcribed spacer (ITS) region. Trichoderma isolates were separated into five distinct species, namely T. harzianum, T. virens, T. brevicompactum, T. tawa, and telomorphic Hypocrea lixii. Among the Trichoderma fungi, T. harzianum was the most frequently (65%) isolated species. Trichoderma harzianum (Isolates of TJ9, 10, and 16) showed antagonistic and inhibitory effects by 61 to 70% on in vitro mycelial growth against three common fungal pathogens of black pepper, P. palmivora, C. gloeosporioides, and F. solani. This study highlights the potential of using native Trichoderma fungi as biocontrol agents in the black pepper integrated disease management program.
{"title":"Characterisation of Trichoderma spp. and Assessment as Biocontrol Using Dual Culture Assay Against Fungi Associated with Black Pepper (Piper nigrum L.) Diseases in Sarawak","authors":"Y. Kwan, Joseph Wilson","doi":"10.33736/bjrst.4358.2022","DOIUrl":"https://doi.org/10.33736/bjrst.4358.2022","url":null,"abstract":"Black pepper (Piper nigrum L.) is one of the most widely used spices in food, beverage, cosmetics, and medicine. Black pepper production has suffered from various fungal diseases. Microbial biological control is an essential part of integrated disease management to reduce the heavy reliance on chemical fungicides. Trichoderma fungi comprise a large group of rhizocompetent filamentous fungi widely used in the biocontrol of plant pathogens. Three field surveys conducted on five black pepper farms in Belaga, Sarawak, identified three fungal diseases: yellowing, black berry, and foot rot. Based on the morphological and molecular characterisation, the identified fungal causal agents were Fusarium solani (yellowing disease), Colletotrichum gloeosporioides (black berry disease), and Phytophthora palmivora (foot rot disease). Twenty isolates of Trichoderma spp. were isolated from secondary forest and Biopark in Bintulu, Sarawak. Trichoderma isolates were characterised based on the morphological characteristics and molecular phylogenetic analysis using the rDNA internal transcribed spacer (ITS) region. Trichoderma isolates were separated into five distinct species, namely T. harzianum, T. virens, T. brevicompactum, T. tawa, and telomorphic Hypocrea lixii. Among the Trichoderma fungi, T. harzianum was the most frequently (65%) isolated species. Trichoderma harzianum (Isolates of TJ9, 10, and 16) showed antagonistic and inhibitory effects by 61 to 70% on in vitro mycelial growth against three common fungal pathogens of black pepper, P. palmivora, C. gloeosporioides, and F. solani. This study highlights the potential of using native Trichoderma fungi as biocontrol agents in the black pepper integrated disease management program.","PeriodicalId":32107,"journal":{"name":"Borneo Journal of Resource Science and Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43207362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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