Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-44
Palaninathan PA, Elias DM, Nagarajan N, Ramakrishnan P, Kumari SL
Pulmonary nocardiosis is a rare infectious disorder mainly affecting immunocompromised patients. It may remain cryptic which gradually progresses in its course. Our patient was a 37 years old immunocompetent female who presented with radiological picture of left upper lobe opacification. She was treated with empirical anti tuberculosis treatment outside, no radiological improvement seen. After she reported here, she was evaluated with bronchoscopy with biopsy and bronchial wash culture showed Nocardia species. She was managed with trimethoprim-sulphamethoxazole and amikacin with regular follow up for 6 months. She had radiological resolution and clinical improvement after 4 months of treatment. Hence we made the diagnosis of this rare infection in immunocompetent individual and successfully treated her, which was mistreated as pulmonary tuberculosis elsewhere.
{"title":"A rare case report of pulmonary nocardiosis mimicking as lung malignancy","authors":"Palaninathan PA, Elias DM, Nagarajan N, Ramakrishnan P, Kumari SL","doi":"10.17727/jmsr.2023/11-44","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-44","url":null,"abstract":"Pulmonary nocardiosis is a rare infectious disorder mainly affecting immunocompromised patients. It may remain cryptic which gradually progresses in its course. Our patient was a 37 years old immunocompetent female who presented with radiological picture of left upper lobe opacification. She was treated with empirical anti tuberculosis treatment outside, no radiological improvement seen. After she reported here, she was evaluated with bronchoscopy with biopsy and bronchial wash culture showed Nocardia species. She was managed with trimethoprim-sulphamethoxazole and amikacin with regular follow up for 6 months. She had radiological resolution and clinical improvement after 4 months of treatment. Hence we made the diagnosis of this rare infection in immunocompetent individual and successfully treated her, which was mistreated as pulmonary tuberculosis elsewhere.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-32
Ranjan A, Banerjee B, Das S, Biswas L, Pal SK
Background: Non-alcoholic fatty liver disease (NAFLD) is often an incidental finding in a large subset of Indian population, who because of their sedentary life style are more prone to ischaemic heart disease (IHD). This study was aimed at determining the association between NAFLD and IHD, with and without traditional risk factors for ischemic heart disease and to determine the relationship between the ischemic heart disease and severity of NAFLD. Materials and methods: It was a prospective comparative study among patients who got admitted in General Medicine ward or visited OPD of our institute from February 2020 to September 2021. Patients selected were divided into two groups- Study arm included patients with clinical features and investigations suggestive of ischemic heart disease. Control arm patients were age (+/-5years) matched people coming to hospital with infections/ illness not affecting liver, and without any history of ischaemic heart disease. We did check for NAFLD in these patients and tried to determine the association with IHD. Results: Significant number of patients (68.5%) had NAFLD in the study arm patients who were admitted for IHD in comparison to only 33.3% (p value-<0.001). Study arm had 31% patients with grade 2 or more NAFLD in comparison to 17% of control arm patients. There was significant association present between IHD and Severity of NAFLD (p value<0.05). Conclusion: NAFLD should be considered a risk factor of IHD, and should prompt clinicians to search for other cardiovascular risk factors and intervene at earliest.
{"title":"A study on association between non alcoholic fatty liver disease and ischemic heart disease in a tertiary care hospital","authors":"Ranjan A, Banerjee B, Das S, Biswas L, Pal SK","doi":"10.17727/jmsr.2023/11-32","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-32","url":null,"abstract":"Background: Non-alcoholic fatty liver disease (NAFLD) is often an incidental finding in a large subset of Indian population, who because of their sedentary life style are more prone to ischaemic heart disease (IHD). This study was aimed at determining the association between NAFLD and IHD, with and without traditional risk factors for ischemic heart disease and to determine the relationship between the ischemic heart disease and severity of NAFLD. Materials and methods: It was a prospective comparative study among patients who got admitted in General Medicine ward or visited OPD of our institute from February 2020 to September 2021. Patients selected were divided into two groups- Study arm included patients with clinical features and investigations suggestive of ischemic heart disease. Control arm patients were age (+/-5years) matched people coming to hospital with infections/ illness not affecting liver, and without any history of ischaemic heart disease. We did check for NAFLD in these patients and tried to determine the association with IHD. Results: Significant number of patients (68.5%) had NAFLD in the study arm patients who were admitted for IHD in comparison to only 33.3% (p value-<0.001). Study arm had 31% patients with grade 2 or more NAFLD in comparison to 17% of control arm patients. There was significant association present between IHD and Severity of NAFLD (p value<0.05). Conclusion: NAFLD should be considered a risk factor of IHD, and should prompt clinicians to search for other cardiovascular risk factors and intervene at earliest.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-34
Pradhan S, Swami A, Rakesh M, Thakuria R
Background: Red blood cell distribution width (RDW) is a measurement of the size variation of red blood cells in circulation. The Global Registry of Acute Coronary Events (GRACE) score is the most commonly used risk-stratification scoring system to predict mortality in acute coronary syndrome for both ST- elevated myocardial infarction (STEMI), non-ST elevated myocardial infarction (NSTEMI). The aim of this study was to assess the easily determinable red blood cell distribution width (RDW) as an early mortality marker of acute myocardial infarction (MI) mortality and the correlation of RDW-CV on admission with GRACE risk score in patients with acute myocardial infarction. Methods: This hospital-based, prospective observational study included 211 patients (mean age 58.15 +/- 12.45 years, male 176) admitted with acute MI (STEMI/NSTEMI) from January 2022 to June 2022. The study population was divided into two groups basing on the 50th percentile of the admission RDW-CV value -13.7. The GRACE score was calculated on admission to predict the GRACE in hospital mortality score within 24 hours of admission. Results: The study found that the higher RDW-CV values were associated with adverse prognostic factors in acute myocardial infarction like increased age, lower systolic blood pressure, heart failure, cardiac arrest, higher cardiac biomarker levels and higher in-hospital mortality (p = 0.049, level of significance p< 0.05) and this correlated with a higher GRACE score (Pearson’s correlation coefficient R= 0.47, P<0.05). Conclusion: Thus, RDW can be used as a useful parameter for predicting in-hospital mortality in acute MI patients.
{"title":"Correlation between admission red cell distribution width and GRACE risk score as an early mortality marker in patients with acute myocardial infarction","authors":"Pradhan S, Swami A, Rakesh M, Thakuria R","doi":"10.17727/jmsr.2023/11-34","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-34","url":null,"abstract":"Background: Red blood cell distribution width (RDW) is a measurement of the size variation of red blood cells in circulation. The Global Registry of Acute Coronary Events (GRACE) score is the most commonly used risk-stratification scoring system to predict mortality in acute coronary syndrome for both ST- elevated myocardial infarction (STEMI), non-ST elevated myocardial infarction (NSTEMI). The aim of this study was to assess the easily determinable red blood cell distribution width (RDW) as an early mortality marker of acute myocardial infarction (MI) mortality and the correlation of RDW-CV on admission with GRACE risk score in patients with acute myocardial infarction. Methods: This hospital-based, prospective observational study included 211 patients (mean age 58.15 +/- 12.45 years, male 176) admitted with acute MI (STEMI/NSTEMI) from January 2022 to June 2022. The study population was divided into two groups basing on the 50th percentile of the admission RDW-CV value -13.7. The GRACE score was calculated on admission to predict the GRACE in hospital mortality score within 24 hours of admission. Results: The study found that the higher RDW-CV values were associated with adverse prognostic factors in acute myocardial infarction like increased age, lower systolic blood pressure, heart failure, cardiac arrest, higher cardiac biomarker levels and higher in-hospital mortality (p = 0.049, level of significance p< 0.05) and this correlated with a higher GRACE score (Pearson’s correlation coefficient R= 0.47, P<0.05). Conclusion: Thus, RDW can be used as a useful parameter for predicting in-hospital mortality in acute MI patients.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-36
Hegde SS, Dharwadkar S, Sukumar P
Background: Work related eye injuries are one of the common causes for preventable vision loss. The aim of the study was to analyse the epidemiological characteristics of work related eye injury in a tertiary care eye hospital in South India. Methodology: This prospective observational study included all patients who reported to ophthalmic outpatient department with work related eye injury during the study period. Results: Out of the 416 patients, majority were males. The injuries were common in younger age group. Metal welding and grinding, and agriculture were the high risk occupations for work related eye injuries. Corneal foreign body was the most common type of eye injury noted. Most of the patients were not using protective eye gear at the time of injury. About 7.2% of the patients had previous history of eye injury at work place. Conclusion: As work related eye injury is common in younger age group early intervention is required in order to avoid permanent visual impairment. Specific interventional programmes should be considered as these workers are prone for recurrent eye injuries. There is a need for more effective preventive measures, especially in metal work, agriculture and carpentry, where increased frequency and worst prognoses of injuries were observed.
{"title":"Work related eye injuries: Epidemiology in a tertiary care eye hospital of South India","authors":"Hegde SS, Dharwadkar S, Sukumar P","doi":"10.17727/jmsr.2023/11-36","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-36","url":null,"abstract":"Background: Work related eye injuries are one of the common causes for preventable vision loss. The aim of the study was to analyse the epidemiological characteristics of work related eye injury in a tertiary care eye hospital in South India. Methodology: This prospective observational study included all patients who reported to ophthalmic outpatient department with work related eye injury during the study period. Results: Out of the 416 patients, majority were males. The injuries were common in younger age group. Metal welding and grinding, and agriculture were the high risk occupations for work related eye injuries. Corneal foreign body was the most common type of eye injury noted. Most of the patients were not using protective eye gear at the time of injury. About 7.2% of the patients had previous history of eye injury at work place. Conclusion: As work related eye injury is common in younger age group early intervention is required in order to avoid permanent visual impairment. Specific interventional programmes should be considered as these workers are prone for recurrent eye injuries. There is a need for more effective preventive measures, especially in metal work, agriculture and carpentry, where increased frequency and worst prognoses of injuries were observed.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135813039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-40
Dave PD, Chauhan M, Sarkar A
Background: Early years of the life are the most crucial period for the physical, mental, social, emotional, language development and lifelong learning. Integrated child development services (ICDS) program maintained its uniqueness as early childhood development program of the world. The objectives of study were to (1) evaluate the ICDS services & its utilization by the beneficiaries & (2) find out differences in health care services between rural & urban anganwadi centres (AWCs). Method: A cross sectional study involving selected 120 AWCs of Jamnagar was carried out using a pro forma having questions related to health care services provided under ICDS. Results: 67% of beneficiaries were regular in pre-school education (PSE). Percentage of mild, moderate & severe malnutrition varied between 82-85%, 7-12% &6-8% in children between 6m-6 years respectively. Referral services, nutrition and health care session, vaccination services, routine healthcare check-up of children at every 1-3 month and Minimum 4 ANC visits were provided in 60%, 82.5%, 62.5%, 75% and 77.5% AWCs respectively. Conclusion: About 2/3rd beneficiaries were regularly attending PSE with higher prevalence in urban than rural AWCs. Percentage of mild, moderate & severe malnutrition varied between 82-85%, 7-12% & 6-8% in children of 6 months to 6 years respectively. Rural AWCs had more ‘mild malnourished’ child & urban AWCs had more ‘severely malnourished child’.
{"title":"Evaluation of healthcare services of integrated childhood development services (ICDS) in a district of Western India","authors":"Dave PD, Chauhan M, Sarkar A","doi":"10.17727/jmsr.2023/11-40","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-40","url":null,"abstract":"Background: Early years of the life are the most crucial period for the physical, mental, social, emotional, language development and lifelong learning. Integrated child development services (ICDS) program maintained its uniqueness as early childhood development program of the world. The objectives of study were to (1) evaluate the ICDS services & its utilization by the beneficiaries & (2) find out differences in health care services between rural & urban anganwadi centres (AWCs). Method: A cross sectional study involving selected 120 AWCs of Jamnagar was carried out using a pro forma having questions related to health care services provided under ICDS. Results: 67% of beneficiaries were regular in pre-school education (PSE). Percentage of mild, moderate & severe malnutrition varied between 82-85%, 7-12% &6-8% in children between 6m-6 years respectively. Referral services, nutrition and health care session, vaccination services, routine healthcare check-up of children at every 1-3 month and Minimum 4 ANC visits were provided in 60%, 82.5%, 62.5%, 75% and 77.5% AWCs respectively. Conclusion: About 2/3rd beneficiaries were regularly attending PSE with higher prevalence in urban than rural AWCs. Percentage of mild, moderate & severe malnutrition varied between 82-85%, 7-12% & 6-8% in children of 6 months to 6 years respectively. Rural AWCs had more ‘mild malnourished’ child & urban AWCs had more ‘severely malnourished child’.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-42
Mallipudi BVP
Surgical management of incisional hernia remains a challenge to surgeons practicing the art of hernia repair. A novel tension free open incisional hernia repair combined with abdominal panniculectomy is described in detail. Our unpublished data of over 200 patients shows a recurrence rate of 2% over a ten-year period with follow up ranging between 2 to 15 years. This is an excellent technique for open incisional hernia repair.
{"title":"Mallipudi hernioplasty: A novel tension free open incisional hernia repair","authors":"Mallipudi BVP","doi":"10.17727/jmsr.2023/11-42","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-42","url":null,"abstract":"Surgical management of incisional hernia remains a challenge to surgeons practicing the art of hernia repair. A novel tension free open incisional hernia repair combined with abdominal panniculectomy is described in detail. Our unpublished data of over 200 patients shows a recurrence rate of 2% over a ten-year period with follow up ranging between 2 to 15 years. This is an excellent technique for open incisional hernia repair.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-38
Kavalakkatt K, Roy ASS, Shibu R, Dhrushith EP, Suseelan A, Biju S
Introduction: Rotating platform (RP) is a form of mobile-bearing design for total knee arthroplasty (TKA). This reduces the polyethylene contact stresses and the forces acting on tibial component and bone cement interfaces. It increases the femoral roll-back and flexion. The objective was to assess the functional outcome of rotating platform design in primary TKA, in patients with osteoarthritis of knee and the association of age and gender with the outcome. Materials and methods: A prospective observational study was done on 42 patients who underwent primary TKA with RP design and followed-up for one year. The outcome was assessed with the Knee Society Score at one year. Data analysis was done using statistical package SPSS V-27.0. Results: The mean age was 64.33(±6.12). The mean pre-op knee clinical score was 30(±6.409), which improved to mean post–op score of 92.17(±5.53). 37(88.1%) had excellent and 5(11.9%) had good results. 7(70%) patients in the age group <60 years, 24(96%) cases of 60–69 years and 6(85.7%) cases of ≥70 years had excellent results. 23(95.8%) females and 14(77.8%) males had excellent knee clinical scores. The mean pre-op knee functional score was 33.81(±9.988), which improved to a mean post–op score of 82.38(±6.917). 36 cases (85.7%) had excellent and 6(14.3%) had good results. 7(70%) patients <60 years, 22(88%) of 60- 69 years and 7(100%) of ≥70 years had excellent results. 21(87.5%) females and 15(83.3%) males had excellent knee functional scores. Conclusion: Total knee arthroplasty with rotating platform design has shown excellent clinical and functional outcome without any serious complications at one year follow-up.
{"title":"Functional outcome of rotating platform total knee arthroplasty - A prospective observational study","authors":"Kavalakkatt K, Roy ASS, Shibu R, Dhrushith EP, Suseelan A, Biju S","doi":"10.17727/jmsr.2023/11-38","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-38","url":null,"abstract":"Introduction: Rotating platform (RP) is a form of mobile-bearing design for total knee arthroplasty (TKA). This reduces the polyethylene contact stresses and the forces acting on tibial component and bone cement interfaces. It increases the femoral roll-back and flexion. The objective was to assess the functional outcome of rotating platform design in primary TKA, in patients with osteoarthritis of knee and the association of age and gender with the outcome. Materials and methods: A prospective observational study was done on 42 patients who underwent primary TKA with RP design and followed-up for one year. The outcome was assessed with the Knee Society Score at one year. Data analysis was done using statistical package SPSS V-27.0. Results: The mean age was 64.33(±6.12). The mean pre-op knee clinical score was 30(±6.409), which improved to mean post–op score of 92.17(±5.53). 37(88.1%) had excellent and 5(11.9%) had good results. 7(70%) patients in the age group <60 years, 24(96%) cases of 60–69 years and 6(85.7%) cases of ≥70 years had excellent results. 23(95.8%) females and 14(77.8%) males had excellent knee clinical scores. The mean pre-op knee functional score was 33.81(±9.988), which improved to a mean post–op score of 82.38(±6.917). 36 cases (85.7%) had excellent and 6(14.3%) had good results. 7(70%) patients <60 years, 22(88%) of 60- 69 years and 7(100%) of ≥70 years had excellent results. 21(87.5%) females and 15(83.3%) males had excellent knee functional scores. Conclusion: Total knee arthroplasty with rotating platform design has shown excellent clinical and functional outcome without any serious complications at one year follow-up.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: Breast cancer accounts for most cancer-related deaths in women with over 2.3 million new breast cancer cases diagnosed every year worldwide. Pim kinases are oncogenic proteins that play important role in various cancers. Pim-1 contains 1.7 kb promoter region without any transcriptional regulation, which is a characteristic feature of a housekeeping or constitutive promoter. This study was carried out to ascertain potential mutations in the promoter region of Pim-1 in breast cancer and also to comprehend the effect of mutations on c-Myc expression as well as on patients’ survival. Methods: Ninety-six Indian subjects with a first diagnosis of breast cancer who underwent surgery at our hospital were recruited in this study. Genomic DNA was isolated from whole blood, amplified and sequenced to identify potential mutations at the promoter region of Pim-1. FFPE sections were used to determine the c-Mycexpression in all the studied groups. Results: We observed a transversion (C>A at –502) and a deletion (–754Cdel) mutation in the promoter region with negligible association with tumor biology and c-Myc expression. Positive c-Myc expression was noted in almost 50% of the studied population with absolute nuclear staining or both cytoplasmic/nuclear staining. As per survival analysis, promoter region mutations did not modulate the patients’ survival, but patients with negative c-Myc expression had better disease-free survival compared with positive c-Myc expression. Conclusion: Our findings identified the presence of mutation in the promoter region of Pim-1 with negligible impact on c-Myc expression in patients with breast cancer.
{"title":"Impact of promoter region mutations of Pim-1 on c-Myc expression as well as survival in patients with breast cancer","authors":"Beevi SS, Anbrasu K, Kannepalli KK, Verma VK, Pillarisetti RR, Madigubba S, Darapuneni RC, Bollineni BR","doi":"10.17727/jmsr.2023/11-35","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-35","url":null,"abstract":"Purpose: Breast cancer accounts for most cancer-related deaths in women with over 2.3 million new breast cancer cases diagnosed every year worldwide. Pim kinases are oncogenic proteins that play important role in various cancers. Pim-1 contains 1.7 kb promoter region without any transcriptional regulation, which is a characteristic feature of a housekeeping or constitutive promoter. This study was carried out to ascertain potential mutations in the promoter region of Pim-1 in breast cancer and also to comprehend the effect of mutations on c-Myc expression as well as on patients’ survival. Methods: Ninety-six Indian subjects with a first diagnosis of breast cancer who underwent surgery at our hospital were recruited in this study. Genomic DNA was isolated from whole blood, amplified and sequenced to identify potential mutations at the promoter region of Pim-1. FFPE sections were used to determine the c-Mycexpression in all the studied groups. Results: We observed a transversion (C>A at –502) and a deletion (–754Cdel) mutation in the promoter region with negligible association with tumor biology and c-Myc expression. Positive c-Myc expression was noted in almost 50% of the studied population with absolute nuclear staining or both cytoplasmic/nuclear staining. As per survival analysis, promoter region mutations did not modulate the patients’ survival, but patients with negative c-Myc expression had better disease-free survival compared with positive c-Myc expression. Conclusion: Our findings identified the presence of mutation in the promoter region of Pim-1 with negligible impact on c-Myc expression in patients with breast cancer.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-41
Murugan R, Kandasamy S, Gunasekaran KP, Ramalingam GS, Kulandaivel AL
Background: Serous fluids are commonly produced in many disease conditions and it is relatively easy to collect it. Subjecting it to analysis will help identify the etiology of the disease process and thereby help the clinicians to plan the treatment strategy appropriately. The application of the international system for reporting serous fluid cytology will further make it easy for the clinicians with its simpler terminologies and clear categorization of entities. Materials and method: All effusion samples that were received from 2020 to 2022 were examined and categorized according to international system for reporting serous fluid cytology. Risk of malignancy (ROM) was also calculated. Results: Among 400 cases, 140 (35%) were pleural fluid, 260 (65%) were ascitic fluid. Among 140 pleural fluid, 8 (5.7%) were ND, 121 (86.4%) were NFM, 2 (1.4%) were AUS, 5(3.6%) were SFM and 4 (2.9%) were MAL. Among the 260 peritoneal fluid, 13 (5%), 226 (86.9%), 9 (3.5%), 9 (3.5%), and 3 (1.1%) were reported as ND, NFM, AUS, SFM and MAL respectively. Risk of Malignancy (ROM) calculated for the cases collected in this study were 0% for ND, 0.9% for NFM, 45.5% for AUS, 71.4% for SFM and 100% for MAL. Conclusion: The international system (TIS) for reporting serous fluid cytopathology is very easy to employ and gives high accuracy with clear diagnostic criteria for each category, hence makes it easy to communicate with the clinicians by employing simple terminologies.
{"title":"Standardized cytopathology reporting of fluids using newly proposed international system for reporting serous fluid cytology - A single institutional experience","authors":"Murugan R, Kandasamy S, Gunasekaran KP, Ramalingam GS, Kulandaivel AL","doi":"10.17727/jmsr.2023/11-41","DOIUrl":"https://doi.org/10.17727/jmsr.2023/11-41","url":null,"abstract":"Background: Serous fluids are commonly produced in many disease conditions and it is relatively easy to collect it. Subjecting it to analysis will help identify the etiology of the disease process and thereby help the clinicians to plan the treatment strategy appropriately. The application of the international system for reporting serous fluid cytology will further make it easy for the clinicians with its simpler terminologies and clear categorization of entities. Materials and method: All effusion samples that were received from 2020 to 2022 were examined and categorized according to international system for reporting serous fluid cytology. Risk of malignancy (ROM) was also calculated. Results: Among 400 cases, 140 (35%) were pleural fluid, 260 (65%) were ascitic fluid. Among 140 pleural fluid, 8 (5.7%) were ND, 121 (86.4%) were NFM, 2 (1.4%) were AUS, 5(3.6%) were SFM and 4 (2.9%) were MAL. Among the 260 peritoneal fluid, 13 (5%), 226 (86.9%), 9 (3.5%), 9 (3.5%), and 3 (1.1%) were reported as ND, NFM, AUS, SFM and MAL respectively. Risk of Malignancy (ROM) calculated for the cases collected in this study were 0% for ND, 0.9% for NFM, 45.5% for AUS, 71.4% for SFM and 100% for MAL. Conclusion: The international system (TIS) for reporting serous fluid cytopathology is very easy to employ and gives high accuracy with clear diagnostic criteria for each category, hence makes it easy to communicate with the clinicians by employing simple terminologies.","PeriodicalId":32890,"journal":{"name":"Journal of Medical and Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135812446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.17727/jmsr.2023/11-45
Priavadhana R, Meenakshisundaram K, Rajeswari T, Kunnumbrath A
The term soft tissue tumors (STT) encompass a wide range of tumors arising from the mesenchymal tissues. In this study, we have retrospectively analysed the various STTs occurring in the Department of Pathology and included all cases with a confirmed histopathological diagnosis of soft tissue lesions received during the study period of January 2018 – December 2019. These cases were further analysed for the site of the lesion, number of lesions, age and gender of the patients and the results tabulated. A total of 207 cases of soft tissue tumors with confirmed histopathological diagnosis were included in this study. The male: female ratio was 1.1:1. It was observed that most of the soft tissue tumors were seen in the 31-40 years age group (33%) followed by the 41-50 years age group (21%). The most common histopathologic subtype of tumors were of adipocytic origin (61%). Malignant morphology was observed in 2.4% of the cases. Soft tissue tumors are not uncommon tumors and account for 5.2% of cases among the routine histopathological specimens. This study is presented here to understand the demography and the spectrum of soft tissue tumors observed in a tertiary care centre.
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