International Journal of Biological Macromolecules最新文献

Heavy metal pollution causes irreversible damage to plants, animals, and humans. Therefore, it is meaningful to develop facile, fast, and efficient strategies for heavy metal ion (HMI) detection. Here, a portable composite film (CPSE) was designed for HMI detection with high sensitivity and wide detection range. The CPSE was prepared by using cellulose nanofibers (CNF)/polyvinyl alcohol (PVA) as the matrix and utilizing modified starch (HPS) to assist disodium calcium ethylenediaminetetraacetate (EDTA-Ca) to form stable complexes with HMI. The R, G and B values (the three primary colors of light) were captured using an image acquisition system to produce an HMI standard color card successfully. Specifically, the composite film can effectively distinguish Cu²⁺, Pb²⁺ and Fe³⁺. The response time of the composite film to HMI was 2-4 s, and the detection ranges of Cu²⁺ and Fe³⁺ were 5-700 ppm and 10-1000 ppm, respectively. Additionally, the synergistic effect of HPS and EDTA-Ca led to the increase in tensile strength (1.59-1.71 times), tear strength (3.29-3.57 times), and glass transition temperature (~ 6 °C) compared to CNF/PVA/HPS and CNF/PVA/EDTA-Ca films. This study confirms the value of CPSE films as materials for HMI detection and suggests innovative ideas for designing similar biomass detection materials in the future.

Cellulose aerogel is a green and biodegradable material, but the high flammability of cellulose aerogel hinders its development. In order to expand the application range of cellulose aerogel, it is necessary to improve the flame retardancy of cellulose aerogel. In this paper, cellulose hydrogels were prepared by decolorizing waste cotton fabrics (WCF) and dissolving them in 1-butyl-3-methylimidazole chloride salt ([Bmim]Cl)/DMSO. The cellulose hydrogels were impregnated in aqueous phytic acid (PA)/acrylamide (AM) solution for a period of time and then heated and treated. A cellulose composite aerogel with excellent flame retardant properties was successfully prepared by using in situ polymerization. The results show that the cellulose composite aerogel has a porous structure and good thermal stability, and the residual carbon content in the air at 800 °C is >2.86 %. At the same time, it has excellent flame retardancy, the ultimate oxygen index (LOL) is >40, and the vertical combustion carbon length is <3.5 cm. The thermal insulation performance has also been improved, and the composite aerogel with a height of 1 cm has a temperature difference of 40.2 °C between the upper and lower surfaces on the 80 °C heating table.

The effects of different electron beam irradiation doses (5 KGy, 10 KGy, 20 KGy) on the complexation of potato starch with four saturated fatty acids with different chain lengths, i.e., lauric acid (LA), myristic acid (MA), palmitic acid (PA), and stearic acid (SA), were investigated, including structural properties, physicochemical properties, digestive properties, and the effect of Bifidobacteria proliferation. The complexing index increased significantly with increasing irradiation dose and showed the following order: 20 KGy > 10 KGy > 5 KGy > native starch. At irradiation dose of 20 KGy, PA (88.75 %) showed the highest complexing index, followed by MA (87.40 %), SA (82.95 %) and LA (72.33 %). The results of microstructure, relative crystallinity, gelatinization enthalpy, contact angle, and resistant starch content in starch-fatty acid complexes were consistent with the complexing index. In vitro digestion indicated that at irradiation dose of 20 KGy, the addition of PA yielded the highest content of resistant starch (50.35 %), followed by MA (49.25 %), SA (47.05 %) and LA (44.72 %). The four complexes were eventually assessed for their effects on Bifidobacteria's proliferation, with PA exerting the strongest proliferative effects, followed by MA, SA and LA. Overall, electron beam irradiation exhibited good application prospects in the field of starchy food processing and functional foods development.

This study involved the fabrication of double-layer intelligent films using chitosan (CS), polyvinyl alcohol (PVA), grape skin anthocyanin (GSA), gellan gum (GG) and selenium nanoparticles (SeNPs). The CS/PVA/GSA layer functioned as the internal indicator layer, and the GG/SeNPs layer acted as the external layer for antioxidant and antimicrobial purposes. SEM, FTIR, XRD, and TGA results confirmed the successful fabrication of double-layer films as well as the presence of hydrogen bonding interaction between the two layers. The tensile strength of double-layer films (8.06 MPa-9.61 MPa) fallen between that of single-layer CS/PVA/GSA film (12.51 MPa) and GG/SeNPs film (1.50 MPa-7.67 MPa). The double-layer films demonstrated good UV-blocking abilities, as well as outstanding antioxidant (ABTS scavenging rate can be up to ~80 %) and antimicrobial properties. Compared with single-layer CS/PVA/GSA film, the double-layer film incorporated with 6.6 wt% SeNPs (CPG/GS2 film) possessed a more rapid and stronger response towards NH₃/acetic acid as well as enhanced storage stability. Furthermore, the CPG/GS2 film can increase the shelf life of strawberries at 25 °C by 4 days, and its visible color change showed strong correlation with the weight loss rate (R² = 0.99) and hardness (R² = 0.98) of strawberries.

Pineapple leaf waste, a byproduct of agricultural processes, was used as a novel raw material to synthesize carbon dots (CDs) through a simple hydrothermal method. The CDs were subsequently incorporated into pineapple stem starch (PSS)-based active food packaging films. The characterization of the CDs and PSS-CDs films was conducted using various techniques, including UV-light spectroscopy, fluorescence spectroscopy, and transmission electron microscopy. The results revealed that the CDs measured 2.36 ± 0.33 nm and exhibited antioxidant and antibacterial activities. The addition of the CDs led to notable enhancements in both mechanical strength and UV-barrier properties. Thus, PSS-CDs packaging film was successfully prepared, with the incorporation of CDs enhancing the antioxidant and antimicrobial properties of the film, thereby extending the shelf-life of fresh pork.

Non-coding RNAs are considered key regulatory factors in biological and reproductive physiological processes in mammals. However, the molecular functions of long noncoding RNAs (lncRNAs) in regulating dynamic uterine development and reproductive capacity during sexual maturation remain unclear. This study analyzed the expression characteristics and molecular functions of lncRNAs in uterine tissues from 20 goats at four specific time points during sexual maturation: day 1 after birth (D1), 2 months (M2), 4 months (M4), and 6 months (M6), finding that stage-specific DE lncRNAs may regulate cell proliferation, apoptosis, hormone secretion, metabolism, and immune response through multiple action modes. Within the lncRNA-miRNA-mRNA network, a novel lncRNA, TCONS_00046732, associated with uterine development, exhibited significantly higher expression during sexual maturity compared to the prepubertal stage, correlating positively with PRLR and negatively with chi-miR-135b-5p. FISH and IF analyses revealed significant co-localization and distinct expression patterns of TCONS_00046732, chi-miR-135b-5p, and PRLR in the endometrial epithelium. Further experiments confirmed that TCONS_00046732 competitively binds to chi-miR-135b-5p to upregulate PRLR, thereby activating the PI3K-Akt signaling pathway, promoting primary endometrial epithelial cell proliferation, G1-to-S phase transition, and inhibiting apoptosis. These findings enhance our understanding of uterine molecular regulation and provide key insights into the molecular basis of goat sexual development.

The Arf gene family is essential for crop growth and development by regulating vesicle transport. However, few studies exist on the role of Arfs in the growth and yield formation of Brassica napus. Here we provide an exhaustive account of the phylogeny and expression of the 66 Arfs in rapeseed. We found that the expansion of Arf gene family is mainly through whole genome duplication, and some genes are loss during the expansion process. Expression analysis revealed that the Arfs in group X, with the exception of BnaC02.ARFA1B, BnaC06.ARFA1A.2, and BnaA07.ARFA1A.2, exhibited high expression levels across various tissues of B. napus at different developmental stages. These results indicate that the Arfss in group X were important in influencing rapeseed growth and development. We have found that Arfs in B. napus may have a more complex regulatory mechanism due to homologous recombination and gene sub-functionalization. Haplotype analysis indicated that Arfs regulate B. napus yield formation. We found high expression of BnaC07.ARFA1A in all tissues, and its overexpression significantly increased rapeseed silique number and yield. The comprehensive analysis will further characterize the functions of Arfs in B. napus and enhance regulatory networks for yield formation in B. napus.

This study developed polyvinyl alcohol (PVA)/carboxymethylcellulose (CMC)-based films, using citric acid (CA) as a non-toxic crosslinking agent, to enhance the shelf life of water-soluble packaging films. Clove essential oil (CEO)-loaded chitosan nanoparticles (CSNPs) were prepared via Pickering emulsion and incorporated into PVA/CMC/CA composite films. The encapsulation of CEO was confirmed by FTIR and optical microscopy. Thermal properties were analyzed using thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), revealing improved thermal stability and a decrease in glass transition temperature (Tg) upon crosslinking. The formation of ester bonds was confirmed by ATR-FTIR and ¹³CNMR. Water contact angle (WCA) measurements showed a decrease in hydrophilicity, enhancing the barrier properties of the films. SEM images demonstrated good dispersion of CSNP/CEO within the matrix, improving mechanical and barrier properties. The films exhibited a 30 % reduction in water vapor permeability and water solubility. Controlled release studies indicated that the composite films sustained CEO release, extending the shelf life of cherry tomatoes. Thus, these PVA/CMC/CA-CSNP/CEO composite films offer strong potential for food preservation applications.

In recent decades, significant advancements have been made in wound healing treatments, mainly due to the development of biopolymer-based hydrogels. These injectable self-healing hydrogels have attracted considerable interest because of their unique attributes, including reversible chemistry, injectability, and printability. Unlike traditional hydrogels, injectable polysaccharide-based self-healing hydrogels offer numerous benefits. They can be tailored to fit individual patients, significantly advancing personalized medicine. Upon injection, these hydrogels transform in situ into a substance that effectively covers the entire lesion in all three dimensions, reaching irregular and deep lesions. Injectable self-healing hydrogels also play a pivotal role in promoting tissue regeneration. Their diffusive and viscoelastic properties allow for the controlled delivery of cells or therapeutics in a spatiotemporal manner, provide mechanical support, and facilitate the local recruitment and modulation of host cells. Consequently, these hydrogels have revolutionized innovative approaches to tissue regeneration and are ideally suited for managing chronic wounds. This review paper presents a comprehensive classification of injectable self-healing hydrogels commonly used in chronic wound repair and provides a detailed analysis of the various applications of injectable self-healing hydrogels in treating chronic wounds, thereby illuminating this rapidly evolving field.

Recombinantly produced collagens present a sustainable, ethical, and safe substitute for collagens derived from natural sources. However, controlling the folding of the recombinant collagens, crucial for replicating the mechanical properties of natural materials, remains a formidable task. Collagen-like proteins from willow sawfly are relatively small and contain no hydroxyprolines, presenting an attractive alternative to the large and post-translationally modified mammalian collagens. Utilizing CD spectroscopy and analytical ultracentrifugation, we demonstrate that recombinant willow sawfly collagen assembles into collagen triple helices in a concentration-dependent manner. Interestingly, we observed that the lower concentration threshold for the folding can be overcome by freezing or adding crowding agents. Microscopy data show that both freezing and the addition of crowding agents induce phase separation. We propose that the increase in local protein concentration during phase separation drives the nucleation-step of collagen folding. Finally, we show that freezing also induces the folding of recombinant human collagen fragments and accelerates the folding of natural bovine collagen, indicating the potential to apply phase separation as a universal mechanism to control the folding of recombinant collagens. We anticipate that the results provide a method to induce the nucleation of collagen folding without any requirements for genetic engineering or crosslinking.