Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137230
As an emerging non-wood resource, moso bamboo has attracted extensive attention because of its short growth cycle and high holocellulose content. However, the internal structure of moso bamboo is more compact than that of wood, leading to higher chemical consumption during the pulping process, which greatly reduces the quality of the extracted fibers. Herein, an innovative pulping system including enzymes and alkali is proposed to achieve higher-quality extraction of moso bamboo fibers. Benefiting from the synergistic effects of high-temperature and alkali-resistant cellulase, xylanase, and laccase, supplemented with alkaline pulping, adequate retention and softening of moso bamboo fibers were ultimately achieved. The sample treated with an enzyme/alkali system resulted in a relative increase in fiber length of 7.19 % and a 31.26 % increase in beating efficiency over alkaline pulping. In addition, the tensile index and tearing index of the paper treated with the enzyme/alkali system reached 50.17 N·m·g−1 and 9.12 mN·m2·g−1, which were 22.52 % and 20.53 % higher than those of the alkaline pulping, respectively. This work provides new insights into the production of high-performance moso bamboo fibers and paper with low energy and alkali consumption.
{"title":"Extraction of high-quality moso bamboo fibers by enzyme/alkali synergistic mechanism","authors":"","doi":"10.1016/j.ijbiomac.2024.137230","DOIUrl":"10.1016/j.ijbiomac.2024.137230","url":null,"abstract":"<div><div>As an emerging non-wood resource, moso bamboo has attracted extensive attention because of its short growth cycle and high holocellulose content. However, the internal structure of moso bamboo is more compact than that of wood, leading to higher chemical consumption during the pulping process, which greatly reduces the quality of the extracted fibers. Herein, an innovative pulping system including enzymes and alkali is proposed to achieve higher-quality extraction of moso bamboo fibers. Benefiting from the synergistic effects of high-temperature and alkali-resistant cellulase, xylanase, and laccase, supplemented with alkaline pulping, adequate retention and softening of moso bamboo fibers were ultimately achieved. The sample treated with an enzyme/alkali system resulted in a relative increase in fiber length of 7.19 % and a 31.26 % increase in beating efficiency over alkaline pulping. In addition, the tensile index and tearing index of the paper treated with the enzyme/alkali system reached 50.17 N·m·g<sup>−1</sup> and 9.12 mN·m<sup>2</sup>·g<sup>−1</sup>, which were 22.52 % and 20.53 % higher than those of the alkaline pulping, respectively. This work provides new insights into the production of high-performance moso bamboo fibers and paper with low energy and alkali consumption.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137226
On construction sites impacted by particulate matter and hazardous gases, portable integrated air filtration equipment with high efficiency, minimal pressure drops and ammonia (NH3) alarms is critical. Triboelectric nanogenerators (TENG) present a sustainable solution by generating self-powered electricity to fulfill these requirements. In this study, we synthesized zeolitic imidazolate framework-8 (ZIF-8) in situ on the surface of titanium carbide (Ti3C2Tx) to create Ti3C2Tx/ZIF-8, grafted it onto cellulose diacetate via tetraethyl orthosilicate, and ultimately developed a cellulose-based nanofibrous membrane through electrospinning, combining it with a negative triboelectric material to construct a self-powered TENG-based mask. The device achieved a balance between a low pressure drop (61 Pa) and high filtration efficiency (99.21 %, 99.71 %, and 99.98 % for PM0.3, PM0.5, and PM1, respectively). Furthermore, the device responds swiftly to NH3; at a concentration of 100 ppm NH3, it achieves a rapid response rate of 83 %, with a response/recovery time as low as 12/14 s. Notably, the device retains its rapid sterilization capability within a short duration (20 min) and demonstrates remarkable stability across its various performance metrics, even after multiple washes. This study presents a novel approach to the development of multi-use, self-powered wearable devices featuring excellent air filtration performance and NH3 detection capabilities.
{"title":"An electrostatically spun cellulose-based self-powered mask with high efficiency air filtration and ammonia sensing","authors":"","doi":"10.1016/j.ijbiomac.2024.137226","DOIUrl":"10.1016/j.ijbiomac.2024.137226","url":null,"abstract":"<div><div>On construction sites impacted by particulate matter and hazardous gases, portable integrated air filtration equipment with high efficiency, minimal pressure drops and ammonia (NH<sub>3</sub>) alarms is critical. Triboelectric nanogenerators (TENG) present a sustainable solution by generating self-powered electricity to fulfill these requirements. In this study, we synthesized zeolitic imidazolate framework-8 (ZIF-8) in situ on the surface of titanium carbide (Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>) to create Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>/ZIF-8, grafted it onto cellulose diacetate via tetraethyl orthosilicate, and ultimately developed a cellulose-based nanofibrous membrane through electrospinning, combining it with a negative triboelectric material to construct a self-powered TENG-based mask. The device achieved a balance between a low pressure drop (61 Pa) and high filtration efficiency (99.21 %, 99.71 %, and 99.98 % for PM<sub>0.3</sub>, PM<sub>0.5</sub>, and PM<sub>1</sub>, respectively). Furthermore, the device responds swiftly to NH<sub>3</sub>; at a concentration of 100 ppm NH<sub>3</sub>, it achieves a rapid response rate of 83 %, with a response/recovery time as low as 12/14 s. Notably, the device retains its rapid sterilization capability within a short duration (20 min) and demonstrates remarkable stability across its various performance metrics, even after multiple washes. This study presents a novel approach to the development of multi-use, self-powered wearable devices featuring excellent air filtration performance and NH<sub>3</sub> detection capabilities.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137055
Sanaz Alizadeh, Modara Nasiri, Navid Farahmandian, Paniz Farshi, Zahra Aliakbar Ahovan, Jila Majidi, Ali Hashemi, Sasha H Shafikhani, Lorenzo Moroni, Mazaher Gholipourmalekabadi
Tissue deterioration and post-injury infections are the primary cause of skin diseases. Tissue engineering has developed various synthetic and natural polymers to generate bioactive scaffolds that can closely replicate the natural extracellular matrix (ECM). Decellularized tissues have emerged as a potential solution for reconstructing cutaneous lesions due to their ability to preserve the intricate protein structure and provide essential functional domains for cellular differentiation. In this study, we selected bovine pericardium and subjected it to diverse decellularization methods to optimize ECM preservation. Polyvinyl alcohol (PVA)/chitosan (CS) infused with two clinically important antibiotics (colistin and meropenem) was directly electrospun onto the decellularized bovine pericardium (DBPS) to endow the dual-layer scaffold (DBPS-Abs) an antibacterial property. Both DBPS-Abs and DBPS demonstrated a consistent 3D microstructure with interlinked pore networks, minimal degradation, and robust mechanical stability. The DBPS-Abs group exhibited a potent antibacterial effect against standard and clinical strains of Escherichia coli. Moreover, implanting the constructs into full-thickness skin wounds in mice confirmed enhanced wound regeneration in cases treated with DBPS-Abs compared to other groups, observed over a 7- and 21-day post-implantation period. These findings highlight DBPS-Abs as a superior antibacterial wound dressing, requiring further clinical evaluations.
{"title":"Engineering of a bilayer antibacterial wound dressing from bovine pericardium and electrospun chitosan/PVA/antibiotics for infectious skin wounds management: An in vitro and in vivo study.","authors":"Sanaz Alizadeh, Modara Nasiri, Navid Farahmandian, Paniz Farshi, Zahra Aliakbar Ahovan, Jila Majidi, Ali Hashemi, Sasha H Shafikhani, Lorenzo Moroni, Mazaher Gholipourmalekabadi","doi":"10.1016/j.ijbiomac.2024.137055","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137055","url":null,"abstract":"<p><p>Tissue deterioration and post-injury infections are the primary cause of skin diseases. Tissue engineering has developed various synthetic and natural polymers to generate bioactive scaffolds that can closely replicate the natural extracellular matrix (ECM). Decellularized tissues have emerged as a potential solution for reconstructing cutaneous lesions due to their ability to preserve the intricate protein structure and provide essential functional domains for cellular differentiation. In this study, we selected bovine pericardium and subjected it to diverse decellularization methods to optimize ECM preservation. Polyvinyl alcohol (PVA)/chitosan (CS) infused with two clinically important antibiotics (colistin and meropenem) was directly electrospun onto the decellularized bovine pericardium (DBPS) to endow the dual-layer scaffold (DBPS-Abs) an antibacterial property. Both DBPS-Abs and DBPS demonstrated a consistent 3D microstructure with interlinked pore networks, minimal degradation, and robust mechanical stability. The DBPS-Abs group exhibited a potent antibacterial effect against standard and clinical strains of Escherichia coli. Moreover, implanting the constructs into full-thickness skin wounds in mice confirmed enhanced wound regeneration in cases treated with DBPS-Abs compared to other groups, observed over a 7- and 21-day post-implantation period. These findings highlight DBPS-Abs as a superior antibacterial wound dressing, requiring further clinical evaluations.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137210
Hydrogel beads from rice milk and blueberry (BB) skins were fabricated as novel bio-based pH-sensitive devices. The encapsulation of BB into rice milk/alginate beads was achieved through a simple methodology. The colourimetric response of beads in different pH media was evaluated along with the proof of reusability, showing appropriate reversibility. The evaluation of the stability of BB-loaded beads in accelerated ageing conditions (4, 25 and 40 °C and under visible/UV light) showed high stability of beads (up to 28 days) even in the presence of harsh conditions. The half-time of cyanidin-3-glucoside decreases at high temperatures and under UV light exposure. The sensitivity to ammonia (NH3) and trimethylamine (TMA), as main spoilage volatiles of protein food products, was evaluated. The detection limits (LOD) for NH3 and TMA were 22.4 ppm and 72.1 ppm, respectively. Finally, the hydrogel beads were applied to monitor the spoilage of minced chicken breast. The colour of the beads, changing from dark reddish to green/yellowish and indicative of a high level of amine, could be detected by the naked eye after 3–5 days. This research proposes a sustainable, low-cost, and simple method to fabricate BB-loaded hydrogel beads as a promising tool for intelligent packaging applications.
{"title":"Blueberry extract loaded into rice milk/alginate-based hydrogels as pH-sensitive systems to monitor the freshness of minced chicken","authors":"","doi":"10.1016/j.ijbiomac.2024.137210","DOIUrl":"10.1016/j.ijbiomac.2024.137210","url":null,"abstract":"<div><div>Hydrogel beads from rice milk and blueberry (BB) skins were fabricated as novel bio-based pH-sensitive devices. The encapsulation of BB into rice milk/alginate beads was achieved through a simple methodology. The colourimetric response of beads in different pH media was evaluated along with the proof of reusability, showing appropriate reversibility. The evaluation of the stability of BB-loaded beads in accelerated ageing conditions (4, 25 and 40 °C and under visible/UV light) showed high stability of beads (up to 28 days) even in the presence of harsh conditions. The half-time of cyanidin-3-glucoside decreases at high temperatures and under UV light exposure. The sensitivity to ammonia (NH<sub>3</sub>) and trimethylamine (TMA), as main spoilage volatiles of protein food products, was evaluated. The detection limits (LOD) for NH<sub>3</sub> and TMA were 22.4 ppm and 72.1 ppm, respectively. Finally, the hydrogel beads were applied to monitor the spoilage of minced chicken breast. The colour of the beads, changing from dark reddish to green/yellowish and indicative of a high level of amine, could be detected by the naked eye after 3–5 days. This research proposes a sustainable, low-cost, and simple method to fabricate BB-loaded hydrogel beads as a promising tool for intelligent packaging applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137158
The development of superwetting membranes is a promising approach for separating emulsified oily wastewater. However, challenges such as low flux without external pressure and membrane fouling have hindered membrane performance. Herein, we fabricated a novel nanofibrous membrane by grafting Co-doped Zr-UiO-66-NH2 (UiO(Zr/Co)) nanoparticles onto carboxylated cellulose nanocrystals (CCNC)-polyacrylonitrile (PAN) mixed matrix electrospinning membrane via chemical bonds through EDC/NHS reaction. CCNC served a dual purpose by enhancing membrane hydrophilicity and providing connection points for UiO(Zr/Co). The as-prepared UiO(Zr/Co)@CCNC/PAN exhibited superhydrophilic/underwater superoleophobic and anti-fouling properties. The membrane demonstrated excellent demulsification and gravity-driven separation capabilities for various oil-in-water emulsions, with superior permeation flux (1588–2557 L m−2 h−1) and separation efficiency (above 99 %). Furthermore, UiO (Zr/Co)@CCNC/PAN could activate peroxomonosulfate (PMS) under visible light to remove both high viscous crude oil-fouling and bio-fouling, exhibiting impressive photocatalytic self-cleaning and antibacterial activity. The generation of reactive radicals (O2−, OH and SO4−) and non-radical (1O2) species in UiO(Zr/Co)@CCNC/PAN+PMS system through multiple pathways was confirmed. Additionally, the band structure of UiO(Zr/Co) and synergistic photocatalytic-PMS activation mechanism were investigated. This work provides new insights into the design and fabrication of MOF modified superwetting nanofibrous membrane with inherent bonding, high permeation flux, anti-fouling and self-cleaning properties.
{"title":"Co-doped Zr-UiO-66-NH2@carboxylated cellulose nanocrystals/PAN membrane for oil/water separation with photocatalysis-PMS synergistic self-cleaning and antibacterial activity","authors":"","doi":"10.1016/j.ijbiomac.2024.137158","DOIUrl":"10.1016/j.ijbiomac.2024.137158","url":null,"abstract":"<div><div>The development of superwetting membranes is a promising approach for separating emulsified oily wastewater. However, challenges such as low flux without external pressure and membrane fouling have hindered membrane performance. Herein, we fabricated a novel nanofibrous membrane by grafting Co-doped Zr-UiO-66-NH<sub>2</sub> (UiO(Zr/Co)) nanoparticles onto carboxylated cellulose nanocrystals (CCNC)-polyacrylonitrile (PAN) mixed matrix electrospinning membrane via chemical bonds through EDC/NHS reaction. CCNC served a dual purpose by enhancing membrane hydrophilicity and providing connection points for UiO(Zr/Co). The as-prepared UiO(Zr/Co)@CCNC/PAN exhibited superhydrophilic/underwater superoleophobic and anti-fouling properties. The membrane demonstrated excellent demulsification and gravity-driven separation capabilities for various oil-in-water emulsions, with superior permeation flux (1588–2557 L m<sup>−2</sup> h<sup>−1</sup>) and separation efficiency (above 99 %). Furthermore, UiO (Zr/Co)@CCNC/PAN could activate peroxomonosulfate (PMS) under visible light to remove both high viscous crude oil-fouling and bio-fouling, exhibiting impressive photocatalytic self-cleaning and antibacterial activity. The generation of reactive radicals (<img>O<sub>2</sub><sup>−</sup>, <img>OH and SO<sub>4</sub><img><sup>−</sup>) and non-radical (<sup>1</sup>O<sub>2</sub>) species in UiO(Zr/Co)@CCNC/PAN+PMS system through multiple pathways was confirmed. Additionally, the band structure of UiO(Zr/Co) and synergistic photocatalytic-PMS activation mechanism were investigated. This work provides new insights into the design and fabrication of MOF modified superwetting nanofibrous membrane with inherent bonding, high permeation flux, anti-fouling and self-cleaning properties.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.136994
A K Priya, Huda M Alghamdi, V Kavinkumar, Khalid Z Elwakeel, Ahmed M Elgarahy
The generation of municipal solid waste is projected to increase from 2.1 billion tonnes in 2023 to 3.8 billion tonnes by 2050. In 2020, the direct global cost of managing this waste was approximately USD 252 billion. When considering additional hidden costs-such as those arising from pollution, adverse health effects, and climate change due to inadequate waste disposal-the total cost escalates to USD 361 billion. Without significant improvements in waste management practices, this figure could nearly double by 2050, reaching an estimated USD 640.3 billion annually. Among municipal solid waste, biowaste accounts for roughly 44 % of the global municipal solid waste, translating to about 840 million tonnes annually. They are widely accessible and economical, offering a cost-effective alternative to traditional treatment materials. Transforming biomass waste into carbon-based materials (e.g., bioaerogels) is a sustainable practice that reduces waste and repurposes it for environmental remediation. This approach not only decreases the volume of waste directed to landfills and mitigates harmful greenhouse gas emissions from decomposition but also aligns with the principles of a circular economy. Furthermore, it supports sustainable development goals by addressing issues such as water scarcity and pollution while promoting waste valorization and resource efficiency. The unique properties of bioaerogels-including their porosity, multi-layered structure, and chemical adaptability-make them highly effective for the remediation of different water pollutants from aquatic bodies. This review article comprehensively delves into multifaceted wastewater remediation strategies -based bioaerogels such as coagulation and flocculation, advanced oxidation processes, membrane filtration, catalytic processes, water disinfection, Oil-water separation, biodegradation, and adsorption. Additionally, it examines different mechanisms of interaction such as surface adsorption, electrostatic interaction, van der Waals forces, ion exchange, surface precipitation, complexation, pore-filling, hydrophobic interactions, and π-π stacking. Moreover, it conducts an integrated techno-economic evaluation to assess their feasibility in wastewater treatment. By valorizing biomass waste, a closed-loop system can be established, where waste is transformed into valuable bioaerogels. This approach not only addresses challenges related to effluent pollution but also generates economic, environmental, and social benefits. Ultimately, the review underscores the transformative potential of bioaerogels in wastewater treatment, emphasizing their crucial role in supporting long-term environmental goals and advancing the principles of resource circularity.
{"title":"From biomass waste to bioaerogels - An alternative sustainable approach for wastewater remediation.","authors":"A K Priya, Huda M Alghamdi, V Kavinkumar, Khalid Z Elwakeel, Ahmed M Elgarahy","doi":"10.1016/j.ijbiomac.2024.136994","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.136994","url":null,"abstract":"<p><p>The generation of municipal solid waste is projected to increase from 2.1 billion tonnes in 2023 to 3.8 billion tonnes by 2050. In 2020, the direct global cost of managing this waste was approximately USD 252 billion. When considering additional hidden costs-such as those arising from pollution, adverse health effects, and climate change due to inadequate waste disposal-the total cost escalates to USD 361 billion. Without significant improvements in waste management practices, this figure could nearly double by 2050, reaching an estimated USD 640.3 billion annually. Among municipal solid waste, biowaste accounts for roughly 44 % of the global municipal solid waste, translating to about 840 million tonnes annually. They are widely accessible and economical, offering a cost-effective alternative to traditional treatment materials. Transforming biomass waste into carbon-based materials (e.g., bioaerogels) is a sustainable practice that reduces waste and repurposes it for environmental remediation. This approach not only decreases the volume of waste directed to landfills and mitigates harmful greenhouse gas emissions from decomposition but also aligns with the principles of a circular economy. Furthermore, it supports sustainable development goals by addressing issues such as water scarcity and pollution while promoting waste valorization and resource efficiency. The unique properties of bioaerogels-including their porosity, multi-layered structure, and chemical adaptability-make them highly effective for the remediation of different water pollutants from aquatic bodies. This review article comprehensively delves into multifaceted wastewater remediation strategies -based bioaerogels such as coagulation and flocculation, advanced oxidation processes, membrane filtration, catalytic processes, water disinfection, Oil-water separation, biodegradation, and adsorption. Additionally, it examines different mechanisms of interaction such as surface adsorption, electrostatic interaction, van der Waals forces, ion exchange, surface precipitation, complexation, pore-filling, hydrophobic interactions, and π-π stacking. Moreover, it conducts an integrated techno-economic evaluation to assess their feasibility in wastewater treatment. By valorizing biomass waste, a closed-loop system can be established, where waste is transformed into valuable bioaerogels. This approach not only addresses challenges related to effluent pollution but also generates economic, environmental, and social benefits. Ultimately, the review underscores the transformative potential of bioaerogels in wastewater treatment, emphasizing their crucial role in supporting long-term environmental goals and advancing the principles of resource circularity.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137234
This study aimed to identify voltage-dependent anion channel 2 (Hdh-VDAC2) and determine its functional role in response to acute thermal stress, H2O2-induced stress, heavy metal toxicity, bacterial and viral infections, and during metamorphosis. Structural analysis confirmed that Hdh-VDAC2 is a pore-forming β-barrel protein. Molecular docking further confirmed the protein-protein interactions of Hdh-VDAC2 with Hdh-BAX, Hdh-caspase 3, and Hdh-BCL2. In the Hdh-VDAC2-inhibited hemocytes (HCY), apoptotic genes (Hdh-caspase-3 and Hdh-BAX) exhibited elevated mRNA expression, while the anti-apoptotic gene (Hdh-BCL2) was downregulated. Further, fluorescent techniques confirmed excessive reactive oxygen species (ROS) production, lower cell viability, elevated caspase 3 activity, and increased DNA fragmentation in Hdh-VDAC2-inhibited HCY, indicating an anti-apoptotic role of Hdh-VDAC2 in Pacific abalone. Transcriptomic analysis revealed differential expression patterns, with upregulation in the digestive gland (DG) and downregulation in the gill (GIL) and HCY when comparing heat-tolerant (HT) versus heat-sensitive (HS) abalone groups. Additionally, both cold and heat stresses induced Hdh-VDAC2 expression. Other environmental factors including H2O2, cadmium, bacteria, and viruses, were also shown to induce Hdh-VDAC2 mRNA expression in the GIL and DG of Pacific abalone. During metamorphosis, the blastula (BLS) stage exhibited higher Hdh-VDAC2 mRNA expression. These findings suggest that Hdh-VDAC2 plays a crucial anti-apoptotic role and may be a biomarker for summer mortality in Pacific abalone.
{"title":"Molecular and structural analyses of voltage-dependent anion channel 2 and its anti-apoptotic function in stress and pollutant resistance in Pacific abalone","authors":"","doi":"10.1016/j.ijbiomac.2024.137234","DOIUrl":"10.1016/j.ijbiomac.2024.137234","url":null,"abstract":"<div><div>This study aimed to identify voltage-dependent anion channel 2 (Hdh-VDAC2) and determine its functional role in response to acute thermal stress, H<sub>2</sub>O<sub>2</sub>-induced stress, heavy metal toxicity, bacterial and viral infections, and during metamorphosis. Structural analysis confirmed that Hdh-VDAC2 is a pore-forming <em>β</em>-barrel protein. Molecular docking further confirmed the protein-protein interactions of Hdh-VDAC2 with Hdh-BAX, Hdh-caspase 3, and Hdh-BCL2. In the Hdh-VDAC2-inhibited hemocytes (HCY), apoptotic genes (<em>Hdh-caspase-3</em> and <em>Hdh-BAX</em>) exhibited elevated mRNA expression, while the anti-apoptotic gene (<em>Hdh-BCL2</em>) was downregulated. Further, fluorescent techniques confirmed excessive reactive oxygen species (ROS) production, lower cell viability, elevated caspase 3 activity, and increased DNA fragmentation in Hdh-VDAC2-inhibited HCY, indicating an anti-apoptotic role of Hdh-VDAC2 in Pacific abalone. Transcriptomic analysis revealed differential expression patterns, with upregulation in the digestive gland (DG) and downregulation in the gill (GIL) and HCY when comparing heat-tolerant (HT) versus heat-sensitive (HS) abalone groups. Additionally, both cold and heat stresses induced <em>Hdh-VDAC2</em> expression. Other environmental factors including H<sub>2</sub>O<sub>2</sub>, cadmium, bacteria, and viruses, were also shown to induce <em>Hdh-VDAC2</em> mRNA expression in the GIL and DG of Pacific abalone. During metamorphosis, the blastula (BLS) stage exhibited higher <em>Hdh-VDAC2</em> mRNA expression. These findings suggest that Hdh-VDAC2 plays a crucial anti-apoptotic role and may be a biomarker for summer mortality in Pacific abalone.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although hydrogels have attracted increasing attention in the stretchable devices, the low adhesion properties and poor environmental adaptation still seriously restrict their development and application. Herein, we focused on the interaction between polymer networks with disperse media and their resultant influence on gel performance, and constructed self-adhesive and environment-tolerant gelatin/polyacrylamide supramolecular-polymer double-network (Gelatin/PAM SP-DN) eutectogels using multiple supramolecular interactions between natural macromolecule and well-designed deep eutectic solvent (DES). The dual networks of Gelatin/PAM SP-DN eutectogels produced significant supramolecular forces with DES, including hydrogen bonding and electrostatic interaction, contributing to enhance the energy dissipation capacity. Additionally, the Gelatin-PAM SP-DN eutectogels were more prone to generate strong bonding force to various substrates, showcasing both in-situ and ex-situ adhesion performance, and even being used for wet and underwater adhesion. The eutectogels revealed excellent environmental tolerance to maintain excellent mechanical flexibility, conductivity and adhesion at high and low temperatures, ensuring the constructed sensor to sensitively and reliably perceive strain, pressure and human motions over a wide temperature range. Also, the eutectogel demonstrated great potential as a temperature sensor. This work opens up a new horizon in the design of multifunctional and environment-tolerant natural macromolecule-based gel materials for flexible electronics, human-machine interaction and health diagnosis.
{"title":"Environment-tolerant, inherently conductive and self-adhesive gelatin-based supramolecular eutectogel for flexible sensor.","authors":"Zhiyuan Qiao, Ying Chen, Hongyu Pan, Jichang Li, Qingkai Meng, Jianfeng Wang, Yanxia Cao, Wanjie Wang, Yanyu Yang","doi":"10.1016/j.ijbiomac.2024.137219","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137219","url":null,"abstract":"<p><p>Although hydrogels have attracted increasing attention in the stretchable devices, the low adhesion properties and poor environmental adaptation still seriously restrict their development and application. Herein, we focused on the interaction between polymer networks with disperse media and their resultant influence on gel performance, and constructed self-adhesive and environment-tolerant gelatin/polyacrylamide supramolecular-polymer double-network (Gelatin/PAM SP-DN) eutectogels using multiple supramolecular interactions between natural macromolecule and well-designed deep eutectic solvent (DES). The dual networks of Gelatin/PAM SP-DN eutectogels produced significant supramolecular forces with DES, including hydrogen bonding and electrostatic interaction, contributing to enhance the energy dissipation capacity. Additionally, the Gelatin-PAM SP-DN eutectogels were more prone to generate strong bonding force to various substrates, showcasing both in-situ and ex-situ adhesion performance, and even being used for wet and underwater adhesion. The eutectogels revealed excellent environmental tolerance to maintain excellent mechanical flexibility, conductivity and adhesion at high and low temperatures, ensuring the constructed sensor to sensitively and reliably perceive strain, pressure and human motions over a wide temperature range. Also, the eutectogel demonstrated great potential as a temperature sensor. This work opens up a new horizon in the design of multifunctional and environment-tolerant natural macromolecule-based gel materials for flexible electronics, human-machine interaction and health diagnosis.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137229
Thaís de Souza Santos, Paulo Vitor França Lemos, Jamille Santos Santana, Felipe Antônio Silva Anias, Denílson de Jesus Assis, Lucas Guimarães Cardoso, Henrique Rodrigues Marcelino, Ederlan Ferreira de Souza, Jania Betânia Alves da Silva, Carolina Oliveira de Souza
The use of residues from petroleum and crop industries is a feasible and sustainable alternative approach for the production of xanthan gum (XG). This study aimed to evaluate the biosynthesis of XG and the resulting final product obtained using Xanthomonas axonopodis pv. manihotis 1182 in a medium containing produced water (PW) and cassava processing residues. The combined use of PW and cassava crop residues was beneficial for XG production, achieving a product yield of 6.80 g L-1. The micrographs of recovered XG revealed the presence of elongated fiber-like microstructures rather than large agglomerates. The X-ray diffraction profiles of recovered xanthan comprised well-defined peaks rather than an amorphous halo. The thermogravimetry profiles revealed the presence of approximately 60 % of remaining solids in recovered xanthan, in contrast to 30 % in the commercial sample. All the samples demonstrated a pseudoplastic behavior; however, the consistency indices of the recovered samples were approximately 50-times lower than those of commercial XG. The emulsification indices of the recovered XG were > 50 % and comparable to those of commercial xanthan. In this study, for the first time, we obtained a complex XG-metal structure possessing a high emulsification capacity and low viscosity.
利用石油和农作物工业残留物生产黄原胶（XG）是一种可行且可持续的替代方法。这项研究旨在评估黄原胶的生物合成，以及在含有生产用水（PW）和木薯加工残渣的培养基中使用黄单胞菌pv.联合使用废水和木薯加工残渣有利于XG的生产，产品产量达到6.80克/升。回收 XG 的显微照片显示存在细长的纤维状微结构，而不是大的团块。回收黄原胶的 X 射线衍射图显示出清晰的峰值，而不是无定形的光晕。热重曲线显示，回收黄原胶中的剩余固体含量约为 60%，而商用样品中的剩余固体含量仅为 30%。所有样品都表现出假塑性，但回收样品的稠度指数比商用黄原胶低约50倍。回收 XG 的乳化指数大于 50%，与商用黄原胶相当。在这项研究中，我们首次获得了具有高乳化能力和低粘度的XG-金属复合结构。
{"title":"Characterization of xanthan gum-metal complexes biosynthesized using a medium containing produced water and cassava processing residues.","authors":"Thaís de Souza Santos, Paulo Vitor França Lemos, Jamille Santos Santana, Felipe Antônio Silva Anias, Denílson de Jesus Assis, Lucas Guimarães Cardoso, Henrique Rodrigues Marcelino, Ederlan Ferreira de Souza, Jania Betânia Alves da Silva, Carolina Oliveira de Souza","doi":"10.1016/j.ijbiomac.2024.137229","DOIUrl":"https://doi.org/10.1016/j.ijbiomac.2024.137229","url":null,"abstract":"<p><p>The use of residues from petroleum and crop industries is a feasible and sustainable alternative approach for the production of xanthan gum (XG). This study aimed to evaluate the biosynthesis of XG and the resulting final product obtained using Xanthomonas axonopodis pv. manihotis 1182 in a medium containing produced water (PW) and cassava processing residues. The combined use of PW and cassava crop residues was beneficial for XG production, achieving a product yield of 6.80 g L<sup>-1</sup>. The micrographs of recovered XG revealed the presence of elongated fiber-like microstructures rather than large agglomerates. The X-ray diffraction profiles of recovered xanthan comprised well-defined peaks rather than an amorphous halo. The thermogravimetry profiles revealed the presence of approximately 60 % of remaining solids in recovered xanthan, in contrast to 30 % in the commercial sample. All the samples demonstrated a pseudoplastic behavior; however, the consistency indices of the recovered samples were approximately 50-times lower than those of commercial XG. The emulsification indices of the recovered XG were > 50 % and comparable to those of commercial xanthan. In this study, for the first time, we obtained a complex XG-metal structure possessing a high emulsification capacity and low viscosity.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ijbiomac.2024.137239
The use of biomimetic mineralization strategy is promising to solve the problem of poor stability and immune effect of subunit antigens. However, non-specifically inducing protein mineralization is still a challenge. we hypothesized that rhamnolipids with both protein and metal binding capacity could be used to develop more functional and biocompatible calcium mineralized nanoparticle (RMCP). The results show that rhamnolipids synergistically enhanced the mineralization of protein with manganese ions and improved 21 % the loading antigens of RMCP compared to manganese calcium phosphate nanoparticles. Transmission electron microscopy (TEM) and Dynamic Light Scattering (DLS) showed particle size of RMCP is 260 ± 12.1 nm with spherical morphology. In vitro experiments have shown that RMCP effectively activate immune cells through the cGAS-STING and NLRP3 pathways and demonstrated a higher level of cytokines in RAW264.7 Macrophages. In vivo, RMCP triggered an increased IgG titer with 16.5-fold IgG2a/IgG1 ratio compared to the aluminum adjuvant which improved the recovery status after challenge in mice. We used biological surfactants for the first time to enhance the biomimetic mineralization process of subunit antigen, which provides a new approach for constructing calcium-based biocompatible antigen delivery vectors, helping to develop a new generation of stable, efficient, and safe subunit vaccines.
{"title":"Immune enhancement of rhamnolipid/manganese calcium phosphate mineralized nanoparticle: A promising subunit antigen delivery system","authors":"","doi":"10.1016/j.ijbiomac.2024.137239","DOIUrl":"10.1016/j.ijbiomac.2024.137239","url":null,"abstract":"<div><div>The use of biomimetic mineralization strategy is promising to solve the problem of poor stability and immune effect of subunit antigens. However, non-specifically inducing protein mineralization is still a challenge. we hypothesized that rhamnolipids with both protein and metal binding capacity could be used to develop more functional and biocompatible calcium mineralized nanoparticle (RMCP). The results show that rhamnolipids synergistically enhanced the mineralization of protein with manganese ions and improved 21 % the loading antigens of RMCP compared to manganese calcium phosphate nanoparticles. Transmission electron microscopy (TEM) and Dynamic Light Scattering (DLS) showed particle size of RMCP is 260 ± 12.1 nm with spherical morphology. In vitro experiments have shown that RMCP effectively activate immune cells through the cGAS-STING and NLRP3 pathways and demonstrated a higher level of cytokines in RAW264.7 Macrophages. In vivo, RMCP triggered an increased IgG titer with 16.5-fold IgG2a/IgG1 ratio compared to the aluminum adjuvant which improved the recovery status after challenge in mice. We used biological surfactants for the first time to enhance the biomimetic mineralization process of subunit antigen, which provides a new approach for constructing calcium-based biocompatible antigen delivery vectors, helping to develop a new generation of stable, efficient, and safe subunit vaccines.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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