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SDC4 protein action and related key genes in nonhealing diabetic foot ulcers based on bioinformatics analysis and machine learning. 基于生物信息学分析和机器学习的糖尿病足溃疡不愈合中的 SDC4 蛋白作用及相关关键基因。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137789
Yungang Hu, Yiwen Wang, Lin Zhi, Lu Yu, Xiaohua Hu, Yuming Shen, Weili Du

Diabetic foot ulcer (DFU) is a complication associated with diabetes characterised by high morbidity, disability, and mortality, involving chronic inflammation and infiltration of multiple immune cells. We aimed to identify the critical genes in nonhealing DFUs using single-cell RNA sequencing, transcriptomic analysis and machine learning. The GSE165816, GSE134431, and GSE143735 datasets were downloaded from the GEO database. We processed and screened the datasets, and identified the cell subsets. Each cell subtype was annotated, and the predominant cell types contributing to the disease were analysed. Key genes were identified using the LASSO regression algorithm, followed by verification of model accuracy and stability. We investigated the molecular mechanisms and changes in signalling pathways associated with this disease using immunoinfiltration analysis, GSEA, and GSVA. Through scRNA-seq analysis, we identified 12 distinct cell clusters and determined that the basalKera cell type was important in disease development. A high accuracy and stability prediction model was constructed incorporating five key genes (TXN, PHLDA2, RPLP1, MT1G, and SDC4). Among these five genes, SDC4 has the strongest correlation and plays an important role in the development of DFU. Our study identified SDC4 significantly associated with nonhealing DFU development, potentially serving as new prevention and treatment strategies for DFU.

糖尿病足溃疡(DFU)是一种与糖尿病相关的并发症,具有高发病率、高致残率和高死亡率的特点,涉及慢性炎症和多种免疫细胞的浸润。我们的目的是利用单细胞 RNA 测序、转录组分析和机器学习来确定不愈合 DFU 的关键基因。我们从 GEO 数据库下载了 GSE165816、GSE134431 和 GSE143735 数据集。我们对数据集进行了处理和筛选,并确定了细胞亚群。我们对每个细胞亚型进行了注释,并分析了导致疾病的主要细胞类型。使用 LASSO 回归算法确定了关键基因,然后验证了模型的准确性和稳定性。我们利用免疫渗透分析、GSEA 和 GSVA 研究了与这种疾病相关的分子机制和信号通路的变化。通过scRNA-seq分析,我们确定了12个不同的细胞群,并确定basalKera细胞类型在疾病发展中非常重要。我们结合五个关键基因(TXN、PHLDA2、RPLP1、MT1G 和 SDC4)构建了一个高准确性和稳定性的预测模型。在这五个基因中,SDC4 的相关性最强,在 DFU 的发展过程中起着重要作用。我们的研究发现,SDC4 与 DFU 的不愈合发展显著相关,有可能成为 DFU 预防和治疗的新策略。
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引用次数: 0
MP-based plant oleogels structuring with various unsaturated oil: Fabrication, characterization, and in-vitro digestion. 以各种不饱和油为结构的 MP 植物油凝胶:制造、表征和体外消化。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137773
Yuan Li, Limin Wang, Lu Xue, Qianfei Jia, Shufen Li, Yuxi Qin, Zijian Wu

Recently, research on the plant-based oleogels, has gained significant attention as a promising approach for oil formulation. In this research, emulsion-templated technique utilizing millet prolamin (MP) as oleogelator was employed for crafting edible oleogels from various vegetable oils of varying degree of unsaturation. The focus of the study was to examine the formation process, structural characteristics, rheological properties, and lipid digestion of these oleogels. The results indicated that MP-based oleogels exhibited uniform morphology and low oil loss. The construction of the oleogel network could be attributed to hydrogen bonding between oleogelator molecules, hydrophobic interactions among the oleogelator molecules and between the oleogelator and oil, as well as van der Waals forces between the oil molecules themselves. Increasing MP concentration formed a denser oleogel network, and exhibited greater elastic moduli (G') and viscous moduli (G''). Additionally, 8 % MP oleogel prepared from flaxseed oil with high linolenic acid showed low oil loss, high hardness and efficient release of free fatty acids. These results indicated the potential of MP as an oleogelator in creating oleogels, and it is promising for fabricating trans-free and low-saturated oleogel-based products.

最近,有关植物性油凝胶的研究作为一种有前途的油配方方法受到了广泛关注。在这项研究中,采用了以小米原粉(MP)为油凝胶剂的乳液催化技术,从不同不饱和度的植物油中提取可食用的油凝胶。研究的重点是考察这些油凝胶的形成过程、结构特征、流变特性和脂质消化。结果表明,基于 MP 的油凝胶具有均匀的形态和较低的油损失。油凝胶网络的构建可归因于油凝胶分子之间的氢键作用、油凝胶分子之间以及油凝胶与油之间的疏水相互作用,以及油分子本身之间的范德华力。增加 MP 浓度可形成更致密的油凝胶网络,并表现出更大的弹性模量(G')和粘性模量(G'')。此外,用亚麻籽油(亚麻酸含量高)制备的 8% MP 油凝胶显示出低油脂损失、高硬度和游离脂肪酸的高效释放。这些结果表明了 MP 作为油凝胶剂在制造油凝胶方面的潜力,并且有望用于制造不含反式脂肪酸和低饱和度的油凝胶产品。
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引用次数: 0
Improving the catalytic activity and stability of Bacillus alcalophilus serine protease BAPB92 by rational design. 通过合理设计提高嗜铝杆菌丝氨酸蛋白酶 BAPB92 的催化活性和稳定性。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137782
Yuan Yuan, Tingting Wei, Jiyu Qiang, Yangxuan Wen, Jing Lu, Yawei Shi, Jian Zhang

The catalytic activity and stability of proteases are essential for their application in the detergent industry. To enhance the catalytic properties of BAPB92, homologous sequence comparison combined with rational design was employed. Six mutants were generated: BAPB92 (A188P), BAPB92 (V262I), BAPB92 (Q239R), BAPB92 (A188P/V262I), BAPB92 (Q239R/V262I), and BAPB92 (Q239R/A188P). Remarkably, the mutant BAPB92 (A188P/V262I) exhibited the most significant improvement, exhibiting a 4.30-fold increase in kcat/Km compared to the wild type, and a 0.75-fold enhancement in thermal stability at 60 °C. The enzymatic activity of BAPB92 (A188P/V262I) reached 6511.81 U/mg, which is 2.95 times higher than that of the wild type BAPB92. Furthermore, the optimal reaction temperature of this mutant increased from 50 °C to 60 °C. The BAPB92 (A188P/V262I) mutant also showed a marked improvement in detergent stability. In sodium tripolyphosphate liquid detergent, its washing efficacy was 17.84 % higher than that of the wild type, and in methyl glycine diacetate liquid detergent, the improvement was 18.51 %. These findings suggest that BAPB92 (A188P/V262I) holds significant potential as a detergent protease in the washing industry. Structural analysis and molecular dynamics simulations further confirmed the enhanced stability of this mutant compared to the wild type. This study provides valuable theoretical insights for the application of the serine protease BAPB92 in detergent formulations.

蛋白酶的催化活性和稳定性对其在洗涤剂行业的应用至关重要。为了提高 BAPB92 的催化特性,我们采用了同源序列比较与合理设计相结合的方法。共产生了六个突变体:BAPB92(A188P)、BAPB92(V262I)、BAPB92(Q239R)、BAPB92(A188P/V262I)、BAPB92(Q239R/V262I)和 BAPB92(Q239R/A188P)。与野生型相比,突变体 BAPB92 (A188P/V262I) 的 kcat/Km 提高了 4.30 倍,在 60 °C 下的热稳定性提高了 0.75 倍。BAPB92(A188P/V262I)的酶活性达到 6511.81 U/mg ,是野生型 BAPB92 的 2.95 倍。此外,该突变体的最适反应温度从 50 ℃ 升至 60 ℃。BAPB92(A188P/V262I)突变体在去污剂稳定性方面也有明显改善。在三聚磷酸钠液体洗涤剂中,其洗涤效果比野生型高出 17.84%;在二乙酸甘氨酸甲酯液体洗涤剂中,其洗涤效果提高了 18.51%。这些研究结果表明,BAPB92(A188P/V262I)作为洗涤蛋白酶在洗涤工业中具有巨大的潜力。结构分析和分子动力学模拟进一步证实,与野生型相比,该突变体的稳定性更强。这项研究为丝氨酸蛋白酶 BAPB92 在洗涤剂配方中的应用提供了宝贵的理论依据。
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引用次数: 0
Elegant approach to intervention of homogalacturonan from the fruits of Ficus pumila L. in colitis: Unraveling the role of methyl esters and acetyl groups. 从薜荔果实中提取的同半乳糖醛酸干预结肠炎的优雅方法:揭示甲酯和乙酰基的作用。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137793
Jie Chen, Ming-Shun Mei, Yue Yu, Yonglin Zhao, Huan Gong, Weihao Chen, Baoyu Qiu, Songshan Shi, Munisa Dilixiati, Shunchun Wang, Huijun Wang

Oral administration of homogalacturonan (HG) has shown significant potential in anti-colitis activity, yet the therapeutic efficacy of naturally sourced HG still requires enhancement. Herein, HG from the fruits of Ficus pumila L. was modified by chemical methods and the intervention effect of modified HG with different degrees of methyl-esterification (DM) and acetylation (DA) on dextran sulfate sodium-induced colitis in mice was explored. Our results indicated that low-DM HG (DM3 and DM25) primarily mitigated colitis by reducing inflammation (TNF-α, IL-1β, IL-17, and IL-6), while high-DM HG (DM54 and DM94) primarily repaired the intestinal barrier. These effects may be attributed to the differential regulation of gut microbiota by HG with varying DM, such as Lachnospiraceae_NK4A136_group, Lactobacillus, Mucispirillum, Escherichia-Shigella, Bifidobacterium, and Bacteroides. Increased DA reduced the solubility of HG, showing limited anti-inflammatory response but unique advantages in intestinal barrier repair and microbiome regulation (Bifidobacterium, Candidatus_Saccharimonas, Lachnospiraceae_NK4A136_group, Mucispirillum, and Escherichia-Shigella). Furthermore, various structural parameters and substitution degrees showed no significant impact on HG's regulation of oxidative stress reactions. This study emphasized the importance of substituent effect in determining HG's functional role, providing a robust foundation for the design and development of functional polysaccharides for the prevention of intestinal inflammation and other related conditions.

口服高半乳糖醛酸(HG)具有显著的抗结肠炎活性,但天然HG的疗效仍有待提高。本文采用化学方法对薜荔果实中的 HG 进行了改性,并探讨了不同甲基酯化(DM)和乙酰化(DA)程度的改性 HG 对葡聚糖硫酸钠诱导的小鼠结肠炎的干预效果。结果表明,低DM HG(DM3和DM25)主要通过减少炎症(TNF-α、IL-1β、IL-17和IL-6)来缓解结肠炎,而高DM HG(DM54和DM94)主要修复肠道屏障。这些效应可能归因于不同DM的HG对肠道微生物群的不同调节,如Lachnospiraceae_NK4A136_group、乳酸杆菌、黏液螺旋体、志贺氏菌、双歧杆菌和乳酸杆菌。增加 DA 会降低 HG 的溶解度,从而显示出有限的抗炎反应,但在肠道屏障修复和微生物群调节方面却具有独特的优势(双歧杆菌、酵母菌、Lachnospiraceae_NK4A136_group、Mucispirillum 和志贺氏菌)。此外,各种结构参数和取代度对 HG 对氧化应激反应的调控没有显著影响。这项研究强调了取代基效应在决定 HG 功能作用方面的重要性,为设计和开发用于预防肠道炎症及其他相关疾病的功能性多糖奠定了坚实的基础。
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引用次数: 0
Engineered PVA-tamarind gum-based biocomposite for sustained ophthalmic delivery of moxifloxacin: Effect of nanocellulose on physicochemical, mechanoelectrical and permeation kinetics. 用于莫西沙星持续眼科给药的基于 PVA-tamarind 胶的工程生物复合材料:纳米纤维素对物理化学、机械电子学和渗透动力学的影响。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137712
Sk Habibullah, Rakesh Swain, Mouli Das, Sisir Kumar Bhuyan, Biswaranjan Mohanty, Subrata Mallick

Widely used polysaccharide-based films in ophthalmic drug delivery have major limitations of inadequate mechanical strength, poor electrical conductivity, and insufficient ocular drug permeability. Moxifloxacin (MFX) biocomposite film of adequate mechanoelectrical properties was developed for sustained ophthalmic drug delivery. Nanocellulose (NC) incorporated (2.5, 5.0, 7.5, and 10.0 %) PVA-tamarind gum-based moxifloxacin composite was prepared using solvent casting method. The addition of NC improved the mechanical properties of the film, demonstrating its ability to strengthen the structure. Stress relaxation (SR) of the film has been augmented (64.67±7.55 to 73.15±0.34 %) due to increased content of NC (0 to 10 %) respectively. Film containing 5 % NC showed the critical edge of tensile strength (11.9±0.39 MPa), and also the threshold limit of electrical conductivity (4.5*107 Ω). The same film exhibited continued drug release as well as erosion-controlled sustained ocular permeation (pH 7.4) and revealed the highest antibacterial activity (ZOI of disc diffusion, cm) with Pseudomonas aeruginosa (4.63±0.15) and Staphylococcus aureus (4.30±0.26) of MFX (≈224 μg). Notably, incorporating NC produced non-irritating and safe for corneal delivery as confirmed by the Draize model test. Our findings suggested that the NC-containing PVA-tamarind gum-based composite film holds a promising approach for sustained ophthalmic delivery of MFX.

眼科给药领域广泛使用的多糖类薄膜存在机械强度不足、导电性差和眼部药物渗透性不足等主要局限性。为实现眼科药物的持续给药,我们开发了具有适当机械电性能的莫西沙星(MFX)生物复合膜。采用溶剂浇铸法制备了含有纳米纤维素(NC)(2.5%、5.0%、7.5% 和 10.0%)的 PVA-tamarind 胶基莫西沙星复合膜。NC 的添加改善了薄膜的机械性能,证明了其增强结构的能力。由于 NC 含量的增加(0% 至 10%),薄膜的应力松弛(SR)分别增加了(64.67±7.55% 至 73.15±0.34%)。含有 5 % NC 的薄膜达到了拉伸强度的临界值(11.9±0.39 兆帕)和导电率的临界值(4.5*107 Ω)。同样的薄膜显示出持续的药物释放以及侵蚀控制的持续眼部渗透(pH 值为 7.4),并显示出对 MFX(≈224 μg)的铜绿假单胞菌(4.63±0.15)和金黄色葡萄球菌(4.30±0.26)具有最高的抗菌活性(盘扩散 ZOI,cm)。值得注意的是,经德雷兹模型试验证实,掺入 NC 不会产生刺激,可安全地向角膜给药。我们的研究结果表明,含 NC 的 PVA-tamarind 胶基复合膜是一种用于持续眼科给药 MFX 的可行方法。
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引用次数: 0
Isolation, characterization, and activity of the polysaccharides in Bulbophyllum kwangtumgense Schltr. Bulbophyllum kwangtumgense Schltr.
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137382
Liuyue Xiao, Jiaojiao Hou, Ping Chen, Xiao Tan, Xiangxiang Qin, Jing Nie, Hua Zhu, Shian Zhong

A microwave-assisted method was used to extract the polysaccharide from Bulbophyllumkwangtumgense Schltr, and the optimum extraction conditions were determined by orthogonal experiments. The purified polysaccharide component BKP-1 had an obvious polysaccharide characteristic structure on the infrared spectrum and was mainly composed of xylose and glucuronic acid at a ratio of 0.568:0.432. Its molecular weight distribution (polydispersity index was 0.58) was uniform with 1.92× 106 Da. The main structural skeleton of BKP-1 was →4)-β-D-Xylp-(1 → [4)-β-D-Xylp-(1]4 → 4)-α-D-GlcAp-(1 → [4)-α-D-GlcAp-(1]2→. BKP-1 could clear certain free radicals, among which the hydroxyl free radical scavenging ability was the best, with a clearance rate of 61.46 %. Furthermore, BKP-1 significantly modulated the secretion of both pro-inflammatory and anti-inflammatory cytokines at a concentration of 125 μg/mL. Notably, the mechanism BKP-1 exhibiting inhibitory effects were related to regulating of the NF-κB signaling pathway.

采用微波辅助法提取了Bulbophyllumkwangtumgense Schltr的多糖,并通过正交实验确定了最佳提取条件。纯化的多糖成分 BKP-1 在红外光谱上具有明显的多糖特征结构,主要由木糖和葡萄糖醛酸组成,二者的比例为 0.568:0.432。其分子量分布均匀(多分散指数为 0.58),为 1.92× 106 Da。BKP-1 的主要结构骨架为→4)-β-D-Xylp-(1→[4)-β-D-Xylp-(1]4→4)-α-D-GlcAp-(1→[4)-α-D-GlcAp-(1]2→。BKP-1 能清除某些自由基,其中清除羟自由基的能力最强,清除率为 61.46%。此外,在 125 μg/mL 的浓度下,BKP-1 还能明显调节促炎和抗炎细胞因子的分泌。值得注意的是,BKP-1 发挥抑制作用的机制与调节 NF-κB 信号通路有关。
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引用次数: 0
Study on the physicochemical properties and immune regulatory mechanism of polysaccharide fraction from Aronia Melanocarpa fruit. 研究黑木耳果实多糖成分的理化性质和免疫调节机制
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137696
Jinxv Dong, Lei Wang, Yutao Bai, Xin Huang, Changbao Chen, Yan Liu

Aronia Melanocarpa (Michx.) Elliott fruit has been extensively used in the food and medicinal fields. This study aimed to analyze the physicochemical properties of a polysaccharide fraction (AMP2) isolated from this fruit for the first time and investigated its immune regulatory mechanism. The physicochemical properties of AMP2 were determined using high-performance gel permeation chromatography, PMP derivatization-high performance liquid chromatography, Ultraviolet spectroscopy, and infrared spectroscopy. The metagenomic technology was applied to investigate the regulatory effects and mechanisms of AMP2 on the gut microbiota of immunosuppressed mice. The results showed that molecular weight of AMP2 was 83,444 Da, which was mainly composed of D-arabinose, D-xylose, D-mannose, D-rhamnose and d-glucose, and both β-type and α-type glycosidic bonds contained in its structure. AMP2 changed the composition of gut microbiota by increasing the number of beneficial and probiotic bacteria, thereby regulated the intestinal mucosal immune system of host. AMP2 improved intestinal immune system response and antimicrobial capacity through positive regulation of the NOD-like receptor signaling pathway and neutrophil extracellular trap formation. The results demonstrate the potential of AMP2 in immune regulation, providing a new perspective for its subsequent development and contributing to the development and application of related health foods.

Aronia Melanocarpa (Michx.) Elliott 果实已被广泛用于食品和医药领域。本研究旨在首次分析从该果实中分离出的一种多糖成分(AMP2)的理化性质,并研究其免疫调节机制。采用高效凝胶渗透色谱法、PMP衍生化-高效液相色谱法、紫外光谱法和红外光谱法测定了AMP2的理化性质。应用元基因组学技术研究了 AMP2 对免疫抑制小鼠肠道微生物群的调控作用和机制。结果表明,AMP2的分子量为83,444 Da,主要由D-阿拉伯糖、D-木糖、D-甘露糖、D-鼠李糖和d-葡萄糖组成,其结构中同时含有β型和α型糖苷键。AMP2 通过增加有益菌和益生菌的数量来改变肠道微生物群的组成,从而调节宿主的肠道粘膜免疫系统。AMP2 通过正向调节 NOD 样受体信号通路和中性粒细胞胞外陷阱的形成,改善了肠道免疫系统的反应和抗菌能力。这些结果证明了 AMP2 在免疫调节方面的潜力,为其后续开发提供了新的视角,并有助于相关保健食品的开发和应用。
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引用次数: 0
Identification and characterization of a rice expansin-like protein with metal-binding properties. 具有金属结合特性的水稻扩张蛋白样蛋白的鉴定和表征。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137791
Khushboo Chawda, Yuvraj Indoliya, Waseem Siddique, Neelam Gautam, Debasis Chakrabarty

Heavy metal (HM) contamination poses significant threat to agricultural productivity. This study identified and characterized Os09g29690 (OsELP), a rice expansin-like protein. We demonstrated OsELP localizes to the cell wall and is upregulated under various abiotic stresses. Sequence analysis revealed a potential metal-binding CXXXC motif in its conserved domain. Heterologous expression of OsELP in yeast mutants (Δacr3 and Δycf1) enhanced metal tolerance under arsenate [As(V)], arsenite [As(III)], and cadmium [Cd] stress. Yeast cells expressing OsELP accumulated higher amounts of As and Cd, suggesting a potential metal-binding mechanism. This was confirmed through site-directed mutagenesis on the conserved cysteine and serine residues within OsELP. Mutants lacking cysteine residues (mutCS) reduced tolerance to As(III) and Cd but enhanced tolerance to As(V), indicating a role of cysteine in As(III) and Cd binding. Conversely, mutants lacking serine residues (mutSA) reduced tolerance to As(V), suggesting serine's involvement in As(V) binding. These findings reveal the roles of cysteine and serine residues in mediating HM tolerance and binding, confirming OsELP as a key player in HM detoxification through cell wall localization and chelation. This study provides novel insights into the molecular mechanisms of HM tolerance in plants, with potential applications in developing crops with enhanced resistance to HM toxicity.

重金属(HM)污染对农业生产力构成重大威胁。本研究发现并鉴定了水稻扩张蛋白样蛋白 Os09g29690(OsELP)。我们证明 OsELP 定位于细胞壁,并在各种非生物胁迫下上调。序列分析揭示了其保守结构域中潜在的金属结合 CXXXC 基序。OsELP在酵母突变体(Δacr3和Δycf1)中的异源表达增强了酵母在砷酸盐[As(V)]、亚砷酸盐[As(III)]和镉[Cd]胁迫下的金属耐受性。表达 OsELP 的酵母细胞积累了更多的砷和镉,这表明其具有潜在的金属结合机制。通过对 OsELP 中保守的半胱氨酸和丝氨酸残基进行定点突变,证实了这一点。缺乏半胱氨酸残基的突变体(mutCS)降低了对As(III)和Cd的耐受性,但增强了对As(V)的耐受性,表明半胱氨酸在As(III)和Cd结合中的作用。相反,缺乏丝氨酸残基的突变体(mutSA)降低了对 As(V)的耐受性,表明丝氨酸参与了 As(V)的结合。这些发现揭示了半胱氨酸和丝氨酸残基在介导 HM 耐受性和结合中的作用,证实 OsELP 是通过细胞壁定位和螯合作用进行 HM 解毒的关键角色。这项研究为了解植物耐受 HM 的分子机制提供了新的视角,有望应用于开发抗 HM 毒性能力更强的作物。
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引用次数: 0
Effectiveness of the injectable hyaluronic acid-based microparticles loaded with cannabidiol on rat sciatic nerve injury model. 含大麻二酚的透明质酸基注射微颗粒对大鼠坐骨神经损伤模型的疗效。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137780
Seyed Mohammad Davachi, Marisol Vazquez, Maryam Soleimani, Zeinab Hajmohammadi, Maryam Mohajer, Seyed Behnamedin Jamei, Mehdi Khanmohammadi, Roghayeh Najafi, Zohre Bagher, Sajad Hassanzadeh

We have developed an innovative peripheral nerve tissue repair approach by designing biomimetic microparticles loaded with cannabidiol (CBD) using horseradish peroxidase-mediated crosslinking within a microfluidic device. This method utilizes a water-in-oil emulsion system where a mixture of phenol-substituted hyaluronic acid (HAPh), CBD, and laccase is channeled into oil flow, forming hydrogel microparticles. The physical properties, such as their swelling rate, mechanical strength, and the sustained release of CBD, emphasize their potential in tissue engineering and drug delivery applications. Cellular proliferation studies within the microparticles demonstrate their cytocompatibility, making them suitable for developing microtissues. The microparticles also served as a controlled release mechanism for CBD-targeted delivery to the injured locations, showcasing the effectiveness and ability to aid in the regeneration of the sciatic nerve tissue. In vivo, histopathological analysis of treated sciatic nerve injuries showed enhanced axonal restoring and remyelination with HAPh microparticles containing CBD in contrast to control groups. Furthermore, microparticles enhanced various functional aspects of locomotor activities, such as functional sciatic index (SFI) values, response to heat stimulation, and muscle mass retention. In conclusion, results indicate that these composite biomimetic microparticles with CBD effectively promote nerve structural restoration and increase the reconstruction process in a sciatic nerve injury model.

我们利用辣根过氧化物酶介导的交联技术,在微流体设备中设计出了装载大麻二酚(CBD)的仿生微颗粒,从而开发出了一种创新的外周神经组织修复方法。该方法利用油包水型乳液系统,将酚取代透明质酸(HAPh)、大麻二酚和漆酶的混合物导入油流,形成水凝胶微颗粒。微颗粒的物理性质,如溶胀率、机械强度和 CBD 的持续释放,都突出了其在组织工程和药物输送应用方面的潜力。微颗粒内的细胞增殖研究证明了它们的细胞相容性,使其适用于开发微组织。微颗粒还可作为一种可控释放机制,将 CBD 靶向输送到受伤部位,展示了其帮助坐骨神经组织再生的有效性和能力。体内坐骨神经损伤的组织病理学分析表明,与对照组相比,含有 CBD 的 HAPh 微颗粒增强了轴突恢复和髓鞘再形成。此外,微颗粒还增强了运动活动的各种功能,如坐骨神经功能指数(SFI)值、对热刺激的反应和肌肉质量保持。总之,研究结果表明,在坐骨神经损伤模型中,这些含有CBD的复合仿生微颗粒能有效促进神经结构的恢复并提高重建进程。
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引用次数: 0
Synergistic and simultaneous removal of heavy metal ions over waste bamboo shoot particles encapsulated carboxymethyl cellulose/gelatin composite hydrogel. 包裹羧甲基纤维素/明胶复合水凝胶的废弃竹笋颗粒协同并同时去除重金属离子。
IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ijbiomac.2024.137578
Ruiqi Zeng, Jiong Zheng, Yong Zuo, Chen Xiao, Yuanting Zhu

Carboxymethyl cellulose (CMC) hydrogels are commonly used for heavy metal removal due to their abundant hydroxyl and carboxyl groups. However, pristine CMC hydrogels always suffer from low gel strength and limited adsorption properties in large-scale applications. In this study, to improve the gel strength and heavy metal ions removal capacity, fish gelatin and bamboo shoot particle (BSP) were introduced to CMC hydrogels, respectively. The formation of the composite hydrogel with enhanced gel strength was primarily driven by hydrogen bonding, which exhibited an increase strain resistance with a critical strain value up to 214.68 %. As expected, the composite hydrogel can effectively remove Cd2+, Hg2+, and Pb2+ from aqueous solutions simultaneously. The physical adsorption process of heavy metals by the composite hydrogel was well described by the pseudo-second-order kinetic model, while the Langmuir model indicated maximum adsorption capacities of 147.7 mg/g for Cd2+, 88.62 mg/g for Hg2+, and 163.89 mg/g for Pb2+. Notably, the composite hydrogel exhibited enhanced recyclability, maintaining its efficacy for up to at least five cycles. This study underscores the potential of using naturally occurring biodegradable materials for the removal of heavy metals, and paved ways for heavy metal removal at industrial levels.

羧甲基纤维素(CMC)水凝胶因含有丰富的羟基和羧基而常用于去除重金属。然而,原始的 CMC 水凝胶在大规模应用中总是存在凝胶强度低和吸附性能有限的问题。为了提高凝胶强度和重金属离子去除能力,本研究在 CMC 水凝胶中分别引入了鱼明胶和竹笋颗粒(BSP)。增强凝胶强度的复合水凝胶的形成主要是由氢键驱动的,它表现出更高的抗应变性,临界应变值高达 214.68 %。正如预期的那样,复合水凝胶能同时有效地去除水溶液中的 Cd2+、Hg2+ 和 Pb2+。伪二阶动力学模型很好地描述了复合水凝胶对重金属的物理吸附过程,而 Langmuir 模型则表明其对 Cd2+、Hg2+ 和 Pb2+ 的最大吸附容量分别为 147.7 mg/g、88.62 mg/g和 163.89 mg/g。值得注意的是,复合水凝胶显示出更强的可回收性,在至少五个周期内都能保持其功效。这项研究强调了利用天然可生物降解材料去除重金属的潜力,并为在工业层面去除重金属铺平了道路。
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International Journal of Biological Macromolecules
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