International Journal of Biological Macromolecules最新文献_第3页

Preparation of acid-modified waste mask-based activated carbon grafted chitosan composites and their efficient removal of uranyl ions

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143431

Xiaoxia He, Chao Zhou, Yifan Meng, Weiting Xiao, Pengfei Yang

Resource utilization of waste masks is of great significance for environmental protection. In this study, acid-modified waste mask-based activated carbon grafted chitosan composites (CSMA) were prepared using waste masks as a carbon source, chemically modified by introducing sulphonic acid groups through sulfuric acid treatment and further loaded with chitosan, and used for the efficient removal of U(VI) from aqueous solutions. Various characterization techniques, including SEM, FTIR, BET, and XPS, were utilized to investigate the microstructure and surface chemistry of CSMA. The experimental data indicate that optimal adsorption efficiency for CSMA about U(VI) is achieved at a pH of 7, utilizing an adsorbent dosage(m/V) of 0.1 g·L^-1, a reaction duration of 6 h, and a temperature of 303 K. Under these conditions, the maximum adsorption capacity reaches 467.93 mg/g. From the kinetic and thermodynamic results of adsorption, it can be seen that the process of CSMA adsorption on uranyl ions was more in line with the Langmuir isotherm model and pseudo-second-order model. Thermodynamic analysis further confirms that this adsorption process is spontaneous and endothermic. In complex aqueous environments containing various co-existing ions, CSMA demonstrates good selective adsorption capabilities. After several testing cycles, CSMA still maintains a high adsorption efficiency for U(VI). Mechanistic studies using XPS/FTIR suggested that U(VI) capture was governed by coordination/chelation with surface functional groups (-NH₂, -OH, -SO₃H). This study not only presents an innovative approach for resource utilization from discarded masks but also offers an efficient and cost-effective solution for removing radioactive nuclides and facilitating environmental remediation.

{"title":"Preparation of acid-modified waste mask-based activated carbon grafted chitosan composites and their efficient removal of uranyl ions","authors":"Xiaoxia He, Chao Zhou, Yifan Meng, Weiting Xiao, Pengfei Yang","doi":"10.1016/j.ijbiomac.2025.143431","DOIUrl":"10.1016/j.ijbiomac.2025.143431","url":null,"abstract":"<div><div>Resource utilization of waste masks is of great significance for environmental protection. In this study, acid-modified waste mask-based activated carbon grafted chitosan composites (CSMA) were prepared using waste masks as a carbon source, chemically modified by introducing sulphonic acid groups through sulfuric acid treatment and further loaded with chitosan, and used for the efficient removal of U(VI) from aqueous solutions. Various characterization techniques, including SEM, FTIR, BET, and XPS, were utilized to investigate the microstructure and surface chemistry of CSMA. The experimental data indicate that optimal adsorption efficiency for CSMA about U(<em>V</em>I) is achieved at a pH of 7, utilizing an adsorbent dosage(<em>m</em>/<em>V</em>) of 0.1 g·L<sup>-1</sup>, a reaction duration of 6 h, and a temperature of 303 K. Under these conditions, the maximum adsorption capacity reaches 467.93 mg/g. From the kinetic and thermodynamic results of adsorption, it can be seen that the process of CSMA adsorption on uranyl ions was more in line with the Langmuir isotherm model and pseudo-second-order model. Thermodynamic analysis further confirms that this adsorption process is spontaneous and endothermic. In complex aqueous environments containing various co-existing ions, CSMA demonstrates good selective adsorption capabilities. After several testing cycles, CSMA still maintains a high adsorption efficiency for U(VI). Mechanistic studies using XPS/FTIR suggested that U(VI) capture was governed by coordination/chelation with surface functional groups (-NH<sub>2</sub>, -OH, -SO<sub>3</sub>H). This study not only presents an innovative approach for resource utilization from discarded masks but also offers an efficient and cost-effective solution for removing radioactive nuclides and facilitating environmental remediation.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143431"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glutaraldehyde-crosslinked magnetic chitosan nanocomposite for efficient Cr(VI) removal: A sustainable approach to aquatic remediation

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143459

Faiz Al-qarhami , A.B. Abdallah , Magdi E. Khalifa , Fathi S. Awad

Hexavalent chromium (Cr(VI)) is a highly toxic pollutant in aquatic environments, posing serious threats to ecosystems and human health. This study aims to develop an effective adsorbent for the removal of Cr(VI) from water. A novel magnetic chitosan-based nanocomposite (Fe₃O₄@MCS) was synthesized by in situ embedding of Fe₃O₄ nanoparticles into a chitosan matrix, crosslinked with glutaraldehyde, and further modified with ammonia via a Schiff base reaction. The material was thoroughly characterized using FTIR, XPS, XRD, SEM, TEM, EDX, and VSM. Adsorption experiments showed that Fe₃O₄@MCS achieved a maximum Cr(VI) uptake of 221.4 mg/g under optimal conditions (pH 4.0, 25 °C, 10 mg dosage, 120 min contact time), with 100 % removal efficiency at initial concentrations up to 50 ppm within just 15 min. The adsorption followed pseudo-second-order kinetics and fitted well with the Langmuir isotherm model (R² = 0.999), indicating monolayer adsorption behavior. The removal mechanism involves electrostatic interactions between HCrO₄^- and protonated amine/hydroxyl groups, followed by Cr(VI) reduction to Cr(III), as confirmed by FTIR and XPS analyses. Fe₃O₄@MCS also demonstrated excellent magnetic separability and reusability, maintaining over 90 % removal efficiency after five adsorption–desorption cycles. These findings highlight Fe₃O₄@MCS as a highly promising adsorbent for Cr(VI) remediation in water treatment applications.

{"title":"Glutaraldehyde-crosslinked magnetic chitosan nanocomposite for efficient Cr(VI) removal: A sustainable approach to aquatic remediation","authors":"Faiz Al-qarhami , A.B. Abdallah , Magdi E. Khalifa , Fathi S. Awad","doi":"10.1016/j.ijbiomac.2025.143459","DOIUrl":"10.1016/j.ijbiomac.2025.143459","url":null,"abstract":"<div><div>Hexavalent chromium (Cr(VI)) is a highly toxic pollutant in aquatic environments, posing serious threats to ecosystems and human health. This study aims to develop an effective adsorbent for the removal of Cr(VI) from water. A novel magnetic chitosan-based nanocomposite (Fe₃O₄@MCS) was synthesized by in situ embedding of Fe₃O₄ nanoparticles into a chitosan matrix, crosslinked with glutaraldehyde, and further modified with ammonia via a Schiff base reaction. The material was thoroughly characterized using FTIR, XPS, XRD, SEM, TEM, EDX, and VSM. Adsorption experiments showed that Fe₃O₄@MCS achieved a maximum Cr(VI) uptake of 221.4 mg/g under optimal conditions (pH 4.0, 25 °C, 10 mg dosage, 120 min contact time), with 100 % removal efficiency at initial concentrations up to 50 ppm within just 15 min. The adsorption followed pseudo-second-order kinetics and fitted well with the Langmuir isotherm model (R<sup>2</sup> = 0.999), indicating monolayer adsorption behavior. The removal mechanism involves electrostatic interactions between HCrO₄<sup>-</sup> and protonated amine/hydroxyl groups, followed by Cr(VI) reduction to Cr(III), as confirmed by FTIR and XPS analyses. Fe₃O₄@MCS also demonstrated excellent magnetic separability and reusability, maintaining over 90 % removal efficiency after five adsorption–desorption cycles. These findings highlight Fe₃O₄@MCS as a highly promising adsorbent for Cr(VI) remediation in water treatment applications.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143459"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The redox homeostasis-reshapable hyaluronic acid-drug conjugate augments chemo-photodynamic therapy

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143465

Wenxia Zhang , Hui Qiao , Jicheng Cui , Dongmei Zhang , Yingqi Li

The tumor microenvironment poses significant challenges to reactive oxygen species treatment, and fails to retain small molecule drugs for extended periods, leading to low treatment efficacy. Here, a hyaluronic acid-based nanoplatform (HCCD) for combined chemotherapy and photodynamic therapy (PDT) is developed. The nanoplatform links chlorin e6 (Ce6) and doxorubicin (DOX) with hyaluronic acid through a glutathione (GSH)-sensitive disulfide bond, resulting their fluorescence quenching. Notably, their fluorescence and cytotoxicity are specifically activated in tumor cells, not normal ones, due to the endogenous GSH-mediated cleavage of disulfide bonds. Furthermore, PDT is activated in tumor cells with 660 nm laser, and GSH consumption reshapes redox homeostasis balance, thereby synergistically enhancing the anti-tumor efficacy with chemotherapy. HCCD predominantly accumulates in tumors in vivo, enabling precise localization and guidance for PDT and chemotherapy. This approach results in a significant reduction in tumor size, by a factor of 11 compared to the control group, with tumors nearly disappearing. Additionally, HCCD with the toll-like receptor 7 agonist imiquimod (R837) and anti-programmed death ligand 1 (anti-PD-L1) activated systemic immune response, suppressing distant tumor growth. Therefore, it's a promising strategy for precise tumor targeting and combination therapy, with potential in immunotherapy to inhibit primary and metastatic tumor growth.

{"title":"The redox homeostasis-reshapable hyaluronic acid-drug conjugate augments chemo-photodynamic therapy","authors":"Wenxia Zhang , Hui Qiao , Jicheng Cui , Dongmei Zhang , Yingqi Li","doi":"10.1016/j.ijbiomac.2025.143465","DOIUrl":"10.1016/j.ijbiomac.2025.143465","url":null,"abstract":"<div><div>The tumor microenvironment poses significant challenges to reactive oxygen species treatment, and fails to retain small molecule drugs for extended periods, leading to low treatment efficacy. Here, a hyaluronic acid-based nanoplatform (HCCD) for combined chemotherapy and photodynamic therapy (PDT) is developed. The nanoplatform links chlorin e6 (Ce6) and doxorubicin (DOX) with hyaluronic acid through a glutathione (GSH)-sensitive disulfide bond, resulting their fluorescence quenching. Notably, their fluorescence and cytotoxicity are specifically activated in tumor cells, not normal ones, due to the endogenous GSH-mediated cleavage of disulfide bonds. Furthermore, PDT is activated in tumor cells with 660 nm laser, and GSH consumption reshapes redox homeostasis balance, thereby synergistically enhancing the anti-tumor efficacy with chemotherapy. HCCD predominantly accumulates in tumors in vivo, enabling precise localization and guidance for PDT and chemotherapy. This approach results in a significant reduction in tumor size, by a factor of 11 compared to the control group, with tumors nearly disappearing. Additionally, HCCD with the toll-like receptor 7 agonist imiquimod (R837) and anti-programmed death ligand 1 (anti-PD-L1) activated systemic immune response, suppressing distant tumor growth. Therefore, it's a promising strategy for precise tumor targeting and combination therapy, with potential in immunotherapy to inhibit primary and metastatic tumor growth.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143465"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual modification of sweet potato starch: Effects of sequence based on ultrasound-assisted nanoprecipitation and OSA esterification for superior functional properties

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143450

Wenyu Lou , Zhenyu Huang , Haidong Xie , Ahsan Hafiz Muhammad , Chao Zhang , Adem Gharsallaoui , Ming Cai , Jian Wang

This study innovatively combined ultrasound-assisted nanoprecipitation with octenyl succinic anhydride (OSA) esterification for dual modification of sweet potato starch (SPS), systematically investigating the impact of modification sequence (nanoprecipitation-first vs. OSA-first) on functional properties. The results showed that OSA-modified nano precipitated SPS (OSA nano SPS) achieved a 10.8 % higher degree of substitution (DS: 0.0184) than nano precipitated OSA-modified SPS (nano OSA SPS, DS: 0.0166), attributed to enhanced OSA accessibility via prior nano structuring. Ultrasonication reduced starch particle size to 77.01 nm and increased amylose content (244.23 mg/g), resulting the increased solubility and the decreased swelling capacity. OSA esterification of SPS was confirmed by FT-IR, with characteristic peaks at 1729 cm^-1 (C=O) and 1564 cm^-1 (-COONa), improved hydrophobicity (90° contact angle) and formed V-type crystalline structures confirmed by XRD. SEM and TEM analyses conclusively demonstrated that OSA nano SPS exhibited structurally integrated nanoparticles with enhanced surface uniformity and controlled size distribution, while nano OSA SPS displayed irregular aggregates compromising structural integrity. OSA nano SPS exhibited superior Pickering emulsion stability, maintaining over 80 % emulsification index after 120 h, with rheological properties surpassing single-modified counterparts. The work first elucidates the sequence-dependent mechanism of dual modifications, demonstrating that nanoprecipitation before esterification optimizes reaction efficiency and emulsion performance. These findings provide a paradigm for designing starch-based delivery systems through controllable multi-step modification strategies.

{"title":"Dual modification of sweet potato starch: Effects of sequence based on ultrasound-assisted nanoprecipitation and OSA esterification for superior functional properties","authors":"Wenyu Lou , Zhenyu Huang , Haidong Xie , Ahsan Hafiz Muhammad , Chao Zhang , Adem Gharsallaoui , Ming Cai , Jian Wang","doi":"10.1016/j.ijbiomac.2025.143450","DOIUrl":"10.1016/j.ijbiomac.2025.143450","url":null,"abstract":"<div><div>This study innovatively combined ultrasound-assisted nanoprecipitation with octenyl succinic anhydride (OSA) esterification for dual modification of sweet potato starch (SPS), systematically investigating the impact of modification sequence (nanoprecipitation-first vs. OSA-first) on functional properties. The results showed that OSA-modified nano precipitated SPS (OSA nano SPS) achieved a 10.8 % higher degree of substitution (DS: 0.0184) than nano precipitated OSA-modified SPS (nano OSA SPS, DS: 0.0166), attributed to enhanced OSA accessibility via prior nano structuring. Ultrasonication reduced starch particle size to 77.01 nm and increased amylose content (244.23 mg/g), resulting the increased solubility and the decreased swelling capacity. OSA esterification of SPS was confirmed by FT-IR, with characteristic peaks at 1729 cm<sup>-1</sup> (C=O) and 1564 cm<sup>-1</sup> (-COONa), improved hydrophobicity (90° contact angle) and formed V-type crystalline structures confirmed by XRD. SEM and TEM analyses conclusively demonstrated that OSA nano SPS exhibited structurally integrated nanoparticles with enhanced surface uniformity and controlled size distribution, while nano OSA SPS displayed irregular aggregates compromising structural integrity. OSA nano SPS exhibited superior Pickering emulsion stability, maintaining over 80 % emulsification index after 120 h, with rheological properties surpassing single-modified counterparts. The work first elucidates the sequence-dependent mechanism of dual modifications, demonstrating that nanoprecipitation before esterification optimizes reaction efficiency and emulsion performance. These findings provide a paradigm for designing starch-based delivery systems through controllable multi-step modification strategies.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143450"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel macromolecule CPD4 suppresses cell proliferation and metastasis of triple-negative breast cancer by targeting ROR1 protein

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143301

Tram M. Ta , Victoria L. Reed , Shradheya R.R. Gupta , Maryam Khan , Nikhil Chandra , Nick Dwyer , Norman Fultang , Indrakant K. Singh , Bela Peethambaran

Breast cancer is the second most common cancer among women in the US, with triple-negative breast cancer (TNBC) accounting for 15–20 % of new diagnoses. TNBC cells lack estrogen and progesterone receptors, and human epidermal growth factor receptor 2, which makes them resistant to standard hormone treatments. Current therapies like chemotherapy and radiation often harm both cancerous and healthy cells, underscoring the need for developing new targeted treatments. ROR1, an oncoprotein that is overexpressed in various cancers, including breast cancer, is minimally present in normal tissues. Targeting ROR1 signaling has been shown to trigger apoptosis and reduce TNBC cell proliferation. A novel macromolecule compound, CPD4, was discovered through in-silico docking for its ability to bind and inhibit the pseudokinase domain of ROR1. In vitro evidence revealed that CPD4 decreases cell viability and induces apoptosis in TNBC cell lines at concentrations of 2–10 μM, while leaving normal breast cells unharmed. CPD4 also blocks migration, invasion, and causes G2/M-phase arrest in TNBC cells. Its mechanism of action involves reducing key downstream markers of ROR1 signaling, particularly the phosphorylation of AKT/GSK3β. In 3D spheroid cultures, CPD4 reduces the size of TNBC spheroids. Moreover, the combination treatment of CPD4 and the standard chemotherapy docetaxel exhibits synergistic efficacy against different TNBC cell lines with a combination index below 0.01. These results suggest that CPD4 holds promise as a therapeutic option for TNBC and could potentially benefit other cancers with ROR1 overexpression.

{"title":"Novel macromolecule CPD4 suppresses cell proliferation and metastasis of triple-negative breast cancer by targeting ROR1 protein","authors":"Tram M. Ta , Victoria L. Reed , Shradheya R.R. Gupta , Maryam Khan , Nikhil Chandra , Nick Dwyer , Norman Fultang , Indrakant K. Singh , Bela Peethambaran","doi":"10.1016/j.ijbiomac.2025.143301","DOIUrl":"10.1016/j.ijbiomac.2025.143301","url":null,"abstract":"<div><div>Breast cancer is the second most common cancer among women in the US, with triple-negative breast cancer (TNBC) accounting for 15–20 % of new diagnoses. TNBC cells lack estrogen and progesterone receptors, and human epidermal growth factor receptor 2, which makes them resistant to standard hormone treatments. Current therapies like chemotherapy and radiation often harm both cancerous and healthy cells, underscoring the need for developing new targeted treatments. ROR1, an oncoprotein that is overexpressed in various cancers, including breast cancer, is minimally present in normal tissues. Targeting ROR1 signaling has been shown to trigger apoptosis and reduce TNBC cell proliferation. A novel macromolecule compound, CPD4, was discovered through <em>in-silico</em> docking for its ability to bind and inhibit the pseudokinase domain of ROR1. <em>In vitro</em> evidence revealed that CPD4 decreases cell viability and induces apoptosis in TNBC cell lines at concentrations of 2–10 μM, while leaving normal breast cells unharmed. CPD4 also blocks migration, invasion, and causes G2/M-phase arrest in TNBC cells. Its mechanism of action involves reducing key downstream markers of ROR1 signaling, particularly the phosphorylation of AKT/GSK3β. In 3D spheroid cultures, CPD4 reduces the size of TNBC spheroids. Moreover, the combination treatment of CPD4 and the standard chemotherapy docetaxel exhibits synergistic efficacy against different TNBC cell lines with a combination index below 0.01. These results suggest that CPD4 holds promise as a therapeutic option for TNBC and could potentially benefit other cancers with ROR1 overexpression.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143301"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyvalent aptamer DNA nanoassembly based near-infrared carbon dots and rolling circle amplification for synergistic therapy of breast cancer

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143343

Gan Ning , Fang Wang , Ruyan Zhang, Huan Du, Tianxin Weng, Xiufeng Wang, Ting Zhou, Guodong Zhang, Zhiqing Zhang

Integrating imaging and therapy is of great significance in the field of tumor treatment. Here, we report a polyvalent aptamer nanoplatform for breast cancer cell treatment based on rolling circle amplification (RCA). The platform consists of a long single-stranded DNA scaffold from RCA and short-stranded DNAs with various functions. One short strand is linked to near-infrared carbon dots (NIR-CDs), another carries a MUC1 aptamer targeting cancer cells, and the third loads small interfering RNA (siRNA) for gene therapy. Doxorubicin (Dox) attaches to the platform via CG base pairs, integrating fluorescence targeting, photothermal therapy (PTT), gene therapy, and chemotherapy. Aptamer enhances breast cancer cell uptake of the platform. NIR-CDs serve as fluorescence imaging agents and are used for PTT. When deoxyribonucleases degrade the nanoplatform, Dox is released and then exerts its chemotherapeutic effect. SiRNA can form the RNA-induced silencing complex to reduce drug tolerance and enhance the efficacy of loaded drugs. The experimental results show that the platform can accumulate in cancer cells, exert a killing effect on them, and meanwhile reduce side effects on normal cells. In summary, this study expands the application of DNA materials in biomedicine by constructing a diagnostic and therapeutic platform that integrates NIR-CDs and RCA.

{"title":"Polyvalent aptamer DNA nanoassembly based near-infrared carbon dots and rolling circle amplification for synergistic therapy of breast cancer","authors":"Gan Ning , Fang Wang , Ruyan Zhang, Huan Du, Tianxin Weng, Xiufeng Wang, Ting Zhou, Guodong Zhang, Zhiqing Zhang","doi":"10.1016/j.ijbiomac.2025.143343","DOIUrl":"10.1016/j.ijbiomac.2025.143343","url":null,"abstract":"<div><div>Integrating imaging and therapy is of great significance in the field of tumor treatment. Here, we report a polyvalent aptamer nanoplatform for breast cancer cell treatment based on rolling circle amplification (RCA). The platform consists of a long single-stranded DNA scaffold from RCA and short-stranded DNAs with various functions. One short strand is linked to near-infrared carbon dots (NIR-CDs), another carries a MUC1 aptamer targeting cancer cells, and the third loads small interfering RNA (siRNA) for gene therapy. Doxorubicin (Dox) attaches to the platform via CG base pairs, integrating fluorescence targeting, photothermal therapy (PTT), gene therapy, and chemotherapy. Aptamer enhances breast cancer cell uptake of the platform. NIR-CDs serve as fluorescence imaging agents and are used for PTT. When deoxyribonucleases degrade the nanoplatform, Dox is released and then exerts its chemotherapeutic effect. SiRNA can form the RNA-induced silencing complex to reduce drug tolerance and enhance the efficacy of loaded drugs. The experimental results show that the platform can accumulate in cancer cells, exert a killing effect on them, and meanwhile reduce side effects on normal cells. In summary, this study expands the application of DNA materials in biomedicine by constructing a diagnostic and therapeutic platform that integrates NIR-CDs and RCA.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143343"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective adsorption of Congo red azo dye from different water and wastewater by using porous Fe3O4-bentonite@chitosan nanocomposite: A multivariate optimization

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143439

Haji Muhammad , Mustafa Tuzen , Asma Siddiqui , Abdul Rehman Umar

In this study, a novel Fe₃O₄-Chitosan@Bentonite (Fe₃O₄-CS/Bent) nanocomposite was synthesized via a green and scalable co-precipitation method for the efficient adsorption of toxic azo dye Congo Red (CR) from wastewater. To analyze the physicochemical changes in nanocomposite before and after CR adsorption SEM-EDS, FTIR, XRD, BET, and VSM were employed. The results revealed that Fe₃O₄-CS/Bent nanocomposite shows significantly enhanced surface area, pore volume, and adsorption capacity compared to pure Fe₃O₄ NPs, making it highly effective for environmental remediation. Despite its lower magnetization due to non-magnetic additives, it retains superparamagnetic properties that facilitate efficient magnetic separation in water treatment. Furthermore, the mesoporous Fe₃O₄-CS/Bent nanocomposite can adsorb CR through electrostatic interactions, hydrogen bondings, and surface interaction under carefully optimized adsorption conditions (pH 5, adsorbent amount of 9 mg, and contact time of 25 min) via multivariate analyses. Subsequently, the adsorption process followed a pseudo-2nd order and Langmuir model, yielding a maximum adsorption capacity of 169 mg/g with 96 % removal efficiency, suggesting a monolayer chemisorption process on a relatively heterogeneous surface. The rate constant was determined to be 0.02 g/g-min suggesting a moderate adsorption rate. Thermodynamic analysis indicated adsorption of CR is spontaneous, exothermic, and feasible at moderate temperatures. Furthermore, Fe₃O₄-CS/Bent exhibits outstanding removal efficiencies in recovery experiments, with 98.6–101.4 % recovery across drinking, tap, canal, and wastewater samples. The adsorbent demonstrated excellent reusability, maintaining over 70 % efficiency after 8th regeneration cycle. These findings establish that Fe₃O₄-CS/Bent nanocomposite is found to be cost-effective environmentally friendly adsorbent for water treatment methods.

{"title":"Effective adsorption of Congo red azo dye from different water and wastewater by using porous Fe3O4-bentonite@chitosan nanocomposite: A multivariate optimization","authors":"Haji Muhammad , Mustafa Tuzen , Asma Siddiqui , Abdul Rehman Umar","doi":"10.1016/j.ijbiomac.2025.143439","DOIUrl":"10.1016/j.ijbiomac.2025.143439","url":null,"abstract":"<div><div>In this study, a novel Fe<sub>3</sub>O<sub>4</sub>-Chitosan@Bentonite (Fe<sub>3</sub>O<sub>4</sub>-CS/Bent) nanocomposite was synthesized via a green and scalable co-precipitation method for the efficient adsorption of toxic azo dye Congo Red (CR) from wastewater. To analyze the physicochemical changes in nanocomposite before and after CR adsorption SEM-EDS, FTIR, XRD, BET, and VSM were employed. The results revealed that Fe<sub>3</sub>O<sub>4</sub>-CS/Bent nanocomposite shows significantly enhanced surface area, pore volume, and adsorption capacity compared to pure Fe<sub>3</sub>O<sub>4</sub> NPs, making it highly effective for environmental remediation. Despite its lower magnetization due to non-magnetic additives, it retains superparamagnetic properties that facilitate efficient magnetic separation in water treatment. Furthermore, the mesoporous Fe<sub>3</sub>O<sub>4</sub>-CS/Bent nanocomposite can adsorb CR through electrostatic interactions, hydrogen bondings, and surface interaction under carefully optimized adsorption conditions (pH 5, adsorbent amount of 9 mg, and contact time of 25 min) via multivariate analyses. Subsequently, the adsorption process followed a pseudo-2nd order and Langmuir model, yielding a maximum adsorption capacity of 169 mg/g with 96 % removal efficiency, suggesting a monolayer chemisorption process on a relatively heterogeneous surface. The rate constant was determined to be 0.02 g/g-min suggesting a moderate adsorption rate. Thermodynamic analysis indicated adsorption of CR is spontaneous, exothermic, and feasible at moderate temperatures. Furthermore, Fe<sub>3</sub>O<sub>4</sub>-CS/Bent exhibits outstanding removal efficiencies in recovery experiments, with 98.6–101.4 % recovery across drinking, tap, canal, and wastewater samples. The adsorbent demonstrated excellent reusability, maintaining over 70 % efficiency after 8th regeneration cycle. These findings establish that Fe<sub>3</sub>O<sub>4</sub>-CS/Bent nanocomposite is found to be cost-effective environmentally friendly adsorbent for water treatment methods.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143439"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astaxanthin encapsulation in microgel-stabilized emulsions and alginate-based microparticles: analysis of in vitro digestion and bioaccessibility

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143464

Ignacio Niizawa, Brenda Y. Espinaco, Guillermo A. Sihufe, Susana E. Zorrilla

Astaxanthin (AX) is a potent natural antioxidant, but its chemical structure makes it susceptible to degradation under digestion conditions. Different delivery systems have been used to protect this bioactive compound as it passes through the gastrointestinal tract to improve its bioaccessibility. This study evaluated three systems under simulated digestive conditions: a soybean oil emulsion containing AX stabilized with whey protein aggregates (MSE), and two alginate-based emulsion microgel particles (EMP1 and EMP2) encapsulating the emulsion. All systems protected the AX emulsion in the gastric phase, with similar lipolysis levels in the intestinal phase. However, AX bioaccessibility differed by the end of the intestinal phase, being higher in the alginate encapsulated systems. Confocal laser scanning microscopy showed faster coalescence of MSE droplets during digestion, which may limit AX bioaccessibility compared to the alginate-encapsulated systems. These findings showed that soybean oil emulsions coated with whey protein aggregates and encapsulated in alginate microparticles improved AX bioaccessibility, suggesting that they may serve as a novel functional food ingredient to effectively deliver lipophilic bioactive compounds.

{"title":"Astaxanthin encapsulation in microgel-stabilized emulsions and alginate-based microparticles: analysis of in vitro digestion and bioaccessibility","authors":"Ignacio Niizawa, Brenda Y. Espinaco, Guillermo A. Sihufe, Susana E. Zorrilla","doi":"10.1016/j.ijbiomac.2025.143464","DOIUrl":"10.1016/j.ijbiomac.2025.143464","url":null,"abstract":"<div><div>Astaxanthin (AX) is a potent natural antioxidant, but its chemical structure makes it susceptible to degradation under digestion conditions. Different delivery systems have been used to protect this bioactive compound as it passes through the gastrointestinal tract to improve its bioaccessibility. This study evaluated three systems under simulated digestive conditions: a soybean oil emulsion containing AX stabilized with whey protein aggregates (MSE), and two alginate-based emulsion microgel particles (EMP1 and EMP2) encapsulating the emulsion. All systems protected the AX emulsion in the gastric phase, with similar lipolysis levels in the intestinal phase. However, AX bioaccessibility differed by the end of the intestinal phase, being higher in the alginate encapsulated systems. Confocal laser scanning microscopy showed faster coalescence of MSE droplets during digestion, which may limit AX bioaccessibility compared to the alginate-encapsulated systems. These findings showed that soybean oil emulsions coated with whey protein aggregates and encapsulated in alginate microparticles improved AX bioaccessibility, suggesting that they may serve as a novel functional food ingredient to effectively deliver lipophilic bioactive compounds.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143464"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of pin to plate atmospheric cold plasma on physicochemical and functional properties of elephant foot yam (Amorphophallus paeoniifolius) starch

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143239

Shilpa S. Pande , Suraj P. Kahar , Uday S. Annapure

This study investigated the effect of atmospheric pressure pin-to-plate cold plasma on physicochemical and functional properties of elephant foot yam starch (EFYS). The isolated EFYS was subjected to the plasma treatment at varying voltages (190 V, 210, V and 230 V) for 5, 10 and 15 min of exposure. The control and treated starches were analysed for pH, amylose content, carboxyl group, paste clarity, water and oil binding property, pasting property, DSC, FTIR, XRD and SEM. There has been a significant reduction (p < 0.05) in pH values of starch from 7.22 to 4.92 and also reduction in turbidity values by 32.48 %. Plasma treatment improved paste clarity and storage stability. However, a significant increase was observed in the oil (1.26–3.80 g/g) and water binding capacity (1.47–1.91 g/g) of the starch. The structural modification, including surface etching, has been confirmed by FTIR and SEM analysis. Plasma treatment significantly increased starch crystallinity, which correlated with higher gelatinization enthalpy, indicating enhanced thermal stability due to plasma induced crosslinking. FTIR data confirmed that cross-linking takes place in samples treated at lower voltages (190Vand 210 V) followed by depolymerization occurring in harshly treated plasma samples (230 V for 15 min).

{"title":"Effect of pin to plate atmospheric cold plasma on physicochemical and functional properties of elephant foot yam (Amorphophallus paeoniifolius) starch","authors":"Shilpa S. Pande , Suraj P. Kahar , Uday S. Annapure","doi":"10.1016/j.ijbiomac.2025.143239","DOIUrl":"10.1016/j.ijbiomac.2025.143239","url":null,"abstract":"<div><div>This study investigated the effect of atmospheric pressure pin-to-plate cold plasma on physicochemical and functional properties of elephant foot yam starch (EFYS). The isolated EFYS was subjected to the plasma treatment at varying voltages (190 V, 210, V and 230 V) for 5, 10 and 15 min of exposure. The control and treated starches were analysed for pH, amylose content, carboxyl group, paste clarity, water and oil binding property, pasting property, DSC, FTIR, XRD and SEM. There has been a significant reduction (<em>p</em> < 0.05) in pH values of starch from 7.22 to 4.92 and also reduction in turbidity values by 32.48 %. Plasma treatment improved paste clarity and storage stability. However, a significant increase was observed in the oil (1.26–3.80 g/g) and water binding capacity (1.47–1.91 g/g) of the starch. The structural modification, including surface etching, has been confirmed by FTIR and SEM analysis. Plasma treatment significantly increased starch crystallinity, which correlated with higher gelatinization enthalpy, indicating enhanced thermal stability due to plasma induced crosslinking. FTIR data confirmed that cross-linking takes place in samples treated at lower voltages (190Vand 210 V) followed by depolymerization occurring in harshly treated plasma samples (230 V for 15 min).</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143239"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ConA-glutamate interactions: New insights into its neuroprotective effect

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules

Pub Date : 2025-04-23 DOI: 10.1016/j.ijbiomac.2025.143463

Renato R. Roma , Fábio S.A. Oliveira , Diógenes G.S. Fernandes , Wanius Garcia , Erica N. Soares , Silvia Lima Costa , Claudener S. Teixeira

L-Glutamate is the primary excitatory neurotransmitter in the brain; excessive levels induce L-glutamate-mediated excitotoxicity, linked to Alzheimer's and Parkinson's. Plant-derived molecules with antioxidant and anti-inflammatory properties that modulate this are of interest. Canavalia ensiformis lectin (ConA) serves as a model lectin for CNS studies. This study aimed to analyze in vitro and in silico the neuroprotective potential of ConA against glutamatergic excitotoxicity and identify the involved protein domain and mechanisms. Native and demetallized ConA were used for cytotoxicity and neuroprotection assays in PC12 cells. Molecular docking and fluorescence spectroscopy were also employed. ConA (1-50 mM) did not show cytotoxicity in PC12 cells and protected them from glutamatergic excitotoxicity at 15.6 μg/mL, significantly increasing cell viability from 80 % to over 90 %. Furthermore, affinity and binding assays indicated that the carbohydrate recognition domain was not involved in neuroprotection; instead, the amino acid-binding site played a crucial role. Our findings conclude that ConA possesses neuroprotective potential against glutamatergic excitotoxicity in PC12 cells via an L-glutamate sequestration mechanism mediated by the amino acid-binding site.

{"title":"ConA-glutamate interactions: New insights into its neuroprotective effect","authors":"Renato R. Roma , Fábio S.A. Oliveira , Diógenes G.S. Fernandes , Wanius Garcia , Erica N. Soares , Silvia Lima Costa , Claudener S. Teixeira","doi":"10.1016/j.ijbiomac.2025.143463","DOIUrl":"10.1016/j.ijbiomac.2025.143463","url":null,"abstract":"<div><div>L-Glutamate is the primary excitatory neurotransmitter in the brain; excessive levels induce L-glutamate-mediated excitotoxicity, linked to Alzheimer's and Parkinson's. Plant-derived molecules with antioxidant and anti-inflammatory properties that modulate this are of interest. <em>Canavalia ensiformis</em> lectin (ConA) serves as a model lectin for CNS studies. This study aimed to analyze in vitro and in silico the neuroprotective potential of ConA against glutamatergic excitotoxicity and identify the involved protein domain and mechanisms. Native and demetallized ConA were used for cytotoxicity and neuroprotection assays in PC12 cells. Molecular docking and fluorescence spectroscopy were also employed. ConA (1-50 mM) did not show cytotoxicity in PC12 cells and protected them from glutamatergic excitotoxicity at 15.6 μg/mL, significantly increasing cell viability from 80 % to over 90 %. Furthermore, affinity and binding assays indicated that the carbohydrate recognition domain was not involved in neuroprotection; instead, the amino acid-binding site played a crucial role. Our findings conclude that ConA possesses neuroprotective potential against glutamatergic excitotoxicity in PC12 cells via an L-glutamate sequestration mechanism mediated by the amino acid-binding site.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"310 ","pages":"Article 143463"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0