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Rapid identification and preliminary evaluation of quality characters of oilseed sunflower by near infrared spectroscopy 近红外光谱法快速鉴定葵花籽品质性状及初步评价
Q3 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.ocsci.2022.08.003
Ling Wang , Lei Wang , Meilian Tan , Haifeng Yu , Jiaomei Wang , Yuxiao Li , Wei Wang , Xingchu Yan , Lijun Wang

To clarify the quality characters, understand the genetic diversity and screen elite lines among different oilseed sunflowers, the contents of crude fat, oleic acid, linoleic acid, palmitic acid and stearic acid of 525 oil sunflowers (including 375 germplasm accessions and 150 inbred lines) were detected by near-infrared spectroscopy (NIRS); the genetic variation and correlation analysis of quality traits were also performed. The results showed that oleic acid and linoleic acid had rich diversities with large variation ranges for each material type. Similar to the relation between crude fat content and palmitic acid content, significantly negative relation with high estimated value existed between oleic acid and linoleic acid content, while stearic acid content positively associated with oleic acid and palmitic acid content. Principal component analysis indicated that 5 quality traits were integrated into 2 principal component factors (linoleic acid negative factor and palmitic acid factor) with the contribution rate of 88.191%, which could be used for evaluating sunflower quality. 525 oilseed sunflowers were clustered into 3 groups with obvious differences of quality characters, materials in Group I had high contents of oleic acid and low crude fat, but the opposite was found in Group III. 59 superior quality accessions were obtained using large-scale and rapid near-infrared spectroscopy, and these excellent materials were verified by the traditional national chemical standard method. This research provided materials and significant reference for sunflower genetic research and quality breeding.

为阐明不同油籽向日葵的品质性状,了解其遗传多样性,筛选优质品系,采用近红外光谱(NIRS)对525份油籽向日葵(包括375份种质资料和150个自交系)的粗脂肪、油酸、亚油酸、棕榈酸和硬脂酸含量进行了检测;并对品质性状进行了遗传变异和相关分析。结果表明,油酸和亚油酸具有丰富的多样性,各物质类型的变化幅度较大。与粗脂肪含量与棕榈酸含量的关系类似,油酸含量与亚油酸含量呈显著负相关且呈高估计值,硬脂酸含量与油酸和棕榈酸含量呈正相关。主成分分析表明,5个品质性状可整合为2个主成分因子(亚油酸负性因子和棕榈酸因子),贡献率为88.191%,可用于向日葵品质评价。525株油籽向日葵聚为3组,品质性状差异明显,ⅰ组材料油酸含量高,粗脂肪含量低,ⅲ组材料油酸含量高,粗脂肪含量低。采用大规模快速近红外光谱法获得了59份优质品,并用传统的国标法对这些优质品进行了验证。本研究为向日葵遗传研究和优质育种提供了材料和重要参考。
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引用次数: 2
Effect of inorganic and organic fertilizers on productivity of groundnut (Arachis hypogaea L.) varieties in East Hararghe, Eastern Ethiopia 无机和有机肥对埃塞俄比亚东部哈拉尔河东部花生品种生产力的影响
Q3 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.ocsci.2022.07.002
Getachew Bekele , Nigussie Dechassa , Tamado Tana

Groundnut (Arachis hypogaea L.) is a high economic value oil crop commonly grown in low-land semi-arid areas of Ethiopia. The crop production is constrained by low soil fertility; however, the nutrient management practice has been given low attention. This study was therefore conducted during the main cropping season of 2015/2016 ​at Babile and Fedis sites of East Hararghe, Ethiopia to evaluate the growth, nodulation, and yield response of groundnut varieties to the application of combined mineral nitrogen, phosphorus, and vermicompost fertilizers, and zinc. Treatments consisted of a factorial combination of three varieties (Ba-ha-Gudo, Ba-ha-Jidu, and Roba); three fertilizers combinations (0: 0 ​kg ​N: P2O5 ha−1 with no VC, 46: 46 ​kg ​N: P2O5 ha−1 with no VC, and 46: 46 ​kg ​N: P2O5 ha−1 with 2.5 ​t VC ha−1); and three rates of Zn (0, 5, and 10 ​kg Zn ha−1) in a randomized complete block design at both sites. The growth, nodulation, yield components, and yield data were subjected to analysis by SAS. The result revealed that plant height was significantly affected by variety and fertilizers combination, the number of branches plant−1 affected by the interaction of variety and fertilizers combination while canopy spread and nodulation parameters were affected by the interaction of variety, fertilizers combination, and Zn rate. Pod plant−1 and hundred seed weight, dry biomass, pod and seed yields, and seed oil content were significantly affected by the interaction of variety, fertilizers combination, and Zn rate. The highest dry biomass (7.26 ​t ​ha−1), pod (3.13 ​t ​ha−1), seed (2.16 ​t ​ha−1) yields and seed oil content (51.3%), net benefit ($1704.2 ​ha-1) with a marginal rate of return of 242% were obtained from variety Ba-ha Gudo received the combined application of 46: 46 ​kg ​N: P2O5 ha−1 and 2.5 ​t VC ha−1 with 5 ​kg Zn ha−1. Therefore, it can be concluded that the Ba-ha Gudo variety is preferably produced using the combined application of 46: 46 ​kg ​N: P2O5 ha−1 and 2.5 ​t VC ha−1 with 5 ​kg Zn ha−1 for high productivity in the study sites and similar agro-ecologies.

花生(arachhis hypogaea L.)是一种高经济价值的油料作物,通常生长在埃塞俄比亚的低地半干旱地区。土壤肥力低制约了作物生产;然而,营养管理实践很少受到重视。因此,本研究于2015/2016年主要种植季在埃塞俄比亚东哈拉尔河的Babile和Fedis站点进行,以评估花生品种对施用矿物氮、磷、蚯蚓复合肥料和锌的生长、结瘤和产量的响应。处理包括三个品种(Ba-ha-Gudo、Ba-ha-Jidu和Roba)的因子组合;3种肥料组合(0:0 kg N: P2O5 ha - 1不含VC, 46: 46 kg N: P2O5 ha - 1不含VC, 46: 46 kg N: P2O5 ha - 1含2.5 t VC ha - 1);在两个地点的随机完全区组设计中,锌的三种浓度(0、5和10 kg Zn ha - 1)。利用SAS软件对生长、结瘤、产量组成和产量数据进行分析。结果表明:株高受品种和肥料组合的显著影响,分枝数受品种和肥料组合的互作影响,而冠层展布和结瘤参数受品种、肥料组合和锌含量的互作影响。品种、肥料组合和锌含量的交互作用显著影响荚果重、百粒重、干生物量、荚果和种子产量以及种子含油量。Ba-ha Gudo品种在41:46 kg N: P2O5 ha-1 + 2.5 t VC ha-1 + 5 kg Zn ha-1的组合施用条件下,干生物量(7.26 t ha-1)、豆荚(3.13 t ha-1)、种子(2.16 t ha-1)产量和种子含油量(51.3%)、净效益(1704.2 ha-1)和边际收益率为242%。综上所述,在研究地点和类似的农业生态环境中,巴哈古多品种在41:46 kg N: P2O5 ha - 1 + 2.5 t VC ha - 1 + 5 kg Zn ha - 1的组合施用条件下可获得较高的产量。
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引用次数: 2
Evaluation of SRAP markers efficiency in genetic diversity of Aspergillus flavus from peanut-cropped soils in China 中国花生种植土壤黄曲霉遗传多样性SRAP标记效率评价
Q3 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.ocsci.2022.08.005
Chushu Zhang , Lifei Zhu , Mian Wang , Yueyi Tang , Haixiang Zhou , Qi Sun , Qiang Yu , Jiancheng Zhang

In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus, we screened out 17 sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs. The size of SRAP fragments ranged from 120 to 2100 bp. Primer pair Me10/Em9 produced the maximum number of polymorphic bands (12 bands), while Me8/Em13 produced the fewest number of polymorphic bands (only 1). Through analysis genetic diversity ability of different sets of primer pairs, the set of 12 primer pairs was selected for SRAP genetic marker of A. flavus. Cluster analysis was performed based on the genetic similarity coefficients, which ranged from 0.53 to 0.89. A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A. flavus samples into 5 main clusters. The results suggested that SRAP marker is a useful molecular technology for the diversity of A. flavus from peanut soils in China.

为了评价SRAP标记对黄曲霉遗传多样性的影响,从150对SRAP引物中筛选出17对能产生清晰、可复制的SRAP条带的引物。SRAP片段的大小在120 ~ 2100 bp之间。Me10/Em9引物产生的多态性条带最多(12条),Me8/Em13引物产生的多态性条带最少(1条)。通过对不同引物对的遗传多样性能力分析,选择了12对引物对作为黄曲霉SRAP遗传标记。聚类分析遗传相似系数为0.53 ~ 0.89。采用算术平均的非加权对群方法,将黄芪样本分为5个主要聚类。结果表明,SRAP标记是中国花生土壤黄曲霉多样性的一种有用的分子技术。
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引用次数: 0
Killing two birds with one stone: ARC-BBBE can simultaneously green control aflatoxin and promote peanut super-nodulation 一举两得:ARC-BBBE可以同时绿色控制黄曲霉毒素和促进花生超结瘤
Q3 Agricultural and Biological Sciences Pub Date : 2022-05-01 DOI: 10.1016/j.ocsci.2022.05.001
Yang Zhou , Xiao-qian Tang , Xiao-feng Yue , Qi Zhang , Pei-wu Li
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引用次数: 1
α-Linolenic acid alleviates aluminium chloride-induced toxicity in PC12 ​cells by activation of PKA-CREB-BDNF signaling pathway α-亚麻酸通过激活PKA-CREB-BDNF信号通路减轻氯化铝对PC12 细胞的毒性
Q3 Agricultural and Biological Sciences Pub Date : 2022-05-01 DOI: 10.1016/j.ocsci.2022.02.008
Huihui Liu, Ling Han, Xiaoyang Xia, Xia Xiang

Aluminum has been associated with neurodegenerative diseases. ALA (α-linolenic acid), an essential dietary component for human health, possesses prominent biological activities. Herein, we aim to explore the neuroprotective effects of ALA on aluminum toxicity and reveal the underlying mechanism. Results show that aluminum chloride (denoted as Al) enabled cell viability decline and apoptosis with oxidative stress and mitochondrial damage in differentiated rat pheochromocytoma cells (PC12) for 24 ​h incubation. Compared with Al (10 ​mmol/L) treatment alone, ALA (50 ​μmol/L) pretreatment for 24 ​h significantly enhanced cell viability by 28.40%, and hindered cell apoptosis by 12.35%, together with recovering redox state balance and alleviating mitochondrial damage. It was measured that ALA treatment upregulated Bcl-2 expression and down-regulated Bax level, accompanied with an expression decline of caspase-3 and caspase-9. Meanwhile, ALA pretreatment was proved to increase protein kinase A (PKA) expression and to promote phosphorylation of cAMP response element-binding protein (p-CREB), resulting in elevation on the level of brain-derived neurotrophic factor (BDNF). The above results showed that ALA attenuated Al toxicity in PC12 ​cells by mediating the PKA-CREB-BDNF signaling pathway.

铝与神经退行性疾病有关。α-亚麻酸(ALA, α-亚麻酸)是人体健康必需的膳食成分,具有显著的生物活性。本研究旨在探讨ALA对铝毒性的神经保护作用,并揭示其机制。结果表明,氯化铝(Al)使分化的大鼠嗜铬细胞瘤(PC12)细胞活性下降,细胞凋亡,并伴有氧化应激和线粒体损伤。与单独使用Al (10 mmol/L)处理相比,ALA (50 μmol/L)预处理24 h可显著提高细胞活力28.40%,抑制细胞凋亡12.35%,恢复氧化还原状态平衡,减轻线粒体损伤。测定ALA处理后Bcl-2表达上调,Bax水平下调,caspase-3、caspase-9表达下降。同时,ALA预处理可增加蛋白激酶A (PKA)表达,促进cAMP反应元件结合蛋白(p-CREB)磷酸化,导致脑源性神经营养因子(BDNF)水平升高。上述结果表明,ALA通过介导PKA-CREB-BDNF信号通路减轻了Al对PC12细胞的毒性。
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引用次数: 1
QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.) 亚麻籽(Linum usitatissmum L.)耐盐候选基因全基因组重测序QTL定位及分析
Q3 Agricultural and Biological Sciences Pub Date : 2022-05-01 DOI: 10.1016/j.ocsci.2022.05.004
Wei Zhao , Yanping Zhang , Jianping Zhang , Yanni Qi , Limin Wang , Zhao Dang , Yaping Xie , Wenjuan Li , Li Zhao

Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield. However, the molecular mechanism of linseed response to salt stress is still unclear. In this study, a salt-tolerant (ST) linseed variety STS and a salt-sensitive (SS) variety DYM were selected as experiment materials. Bulk segregation analysis and whole-genome resequencing technologies were performed to map salt tolerance quantitative trait loci (QTL). A total of 38,625 QTL loci were identified. Fifteen genes (which were not annotated in the reference genome) were identified within a 2.597 ​Mb region in chromosome 1. Two salt tolerance candidate genes Lus.o.m.scaffold91.141 and Lus.o.m. Scaffold1.14 encoding WD40 and cytochrome P450 were identified by predicting protein functions. Previous studies showed that WD40 and cytochrome P450 could significantly improve plant salt stress tolerance. In this paper, results showed that Lus.o.m.scaffold91.141 and Lus.o.m. Scaffold1.14 might be involved in response to salt stress in lineseed. The fine mapping and functional analysis of these genes provide a molecular breeding basis for the genetic improvement of high salt-tolerant linseed varieties.

土壤盐碱化不利于亚麻的生长发育,最终导致产量下降。然而,亚麻籽对盐胁迫反应的分子机制尚不清楚。以耐盐亚麻籽品种STS和盐敏感亚麻籽品种DYM为试验材料。采用整体分离分析和全基因组重测序技术定位耐盐数量性状位点(QTL)。共鉴定出38625个QTL位点。在1号染色体的2.597 Mb区域内鉴定出15个基因(参考基因组中未注释)。两个耐盐候选基因Lus.o.m.scaffold91.141和Lus.o.m。通过预测蛋白功能鉴定编码WD40和细胞色素P450的支架1.14。已有研究表明,WD40和细胞色素P450能显著提高植物的耐盐性。结果表明:Lus.o.m.scaffold91.141和Lus.o.m。Scaffold1.14可能参与了亚麻种子对盐胁迫的响应。这些基因的精细定位和功能分析为高耐盐亚麻籽品种的遗传改良提供了分子育种依据。
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引用次数: 1
Triple test cross analysis for seed yield and its components in sesame under water stress conditions 水分胁迫条件下芝麻籽粒产量及其组成因素的三重试验交叉分析
Q3 Agricultural and Biological Sciences Pub Date : 2022-05-01 DOI: 10.1016/j.ocsci.2022.02.007
Mohamed Ali Abdelsatar , Yousrya S.A. Metwally , Ibthal S. El-Demardash

Water deficit is a limiting factor in sesame cultivars yield, thus identification of genetic mechanisms of sesame traits under water stress conditions is essential to development of water stress tolerant genotypes. Hence, the triple test cross (TTC) analysis in F2 population of the sesame cross (NA76 ​× ​NA54) was used. Since, 30 TTC families with their 13 respective parents were evaluated during the summer season 2020 under two irrigation treatments. i.e., normal irrigation with 5952.38 ​m3 ​hm−2 of applied water, and water stress with 2976.19 ​m3 ​hm−2 of applied water. This was implemented at the experimental field of Kafr El Hamam/Sharkia, Agricultural Research Station, Agricultural Research Center (ARC), Egypt. Additive (D) and dominance (H) gene actions as well as epistatic and its two components of additive ​× ​additive, additive ​× ​dominance plus dominance ​× ​dominance were involved in the inheritance of all studied traits under both irrigation treatments and their combined analysis. The degree of dominance (H/D)0.5 verified the presence of partial dominance in most cases. The correlation coefficient between sums (additive) and differences (dominance) was non-significant, showing that dominant genes between lines were ambidirectional. The moderately water stress tolerant TTC families as the best selected families were found in families 22, 25, 10, 12, and 15. Therefore, postponing selection in these selected families to later segregating generations for all studied traits would be effective to exploit the positive effects of additive ​× ​additive epistasis.

水分亏缺是制约芝麻产量的一个重要因素,研究水分胁迫条件下芝麻性状的遗传机制对培育耐水胁迫基因型具有重要意义。因此,对芝麻杂交品种(NA76 × NA54)的F2群体进行三重检验杂交(TTC)分析。因此,在2020年夏季,对30个TTC家庭及其13名父母进行了两种灌溉处理的评估。即正常灌溉用水量为5952.38 m3 hm−2,水分胁迫用水量为2976.19 m3 hm−2。这是在埃及农业研究中心(ARC)农业研究站Kafr El Hamam/Sharkia试验田实施的。加性(D)和显性(H)基因的作用以及上位性及其加性×加性、加性×显性+显性×显性两个组成部分参与了两种灌溉处理及其组合分析下所有性状的遗传。优势度(H/D)0.5证实多数情况下存在部分优势。和(加性)与差(显性)之间的相关系数不显著,表明系间显性基因具有双向性。中等耐水分胁迫的TTC家系分别为家系22、25、10、12和15。因此,在这些被选择的家族中,将所有被研究性状的选择推迟到较晚的分离代,将有效地利用加性×加性上位的积极作用。
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引用次数: 0
Antioxidant metabolic system and comparative proteomics analysis in winter turnip rape (Brassica rapa L.) under cold stress 低温胁迫下冬季芜菁油菜抗氧化代谢系统及比较蛋白质组学分析
Q3 Agricultural and Biological Sciences Pub Date : 2022-05-01 DOI: 10.1016/j.ocsci.2022.05.002
Xiucun Zeng , Li Ma , Jinhai Yuan , Yaping Xie , Tianxia Guan , Xiaoqin Wang , Guotai Ma , Yaozhao Xu , Wancang Sun

Winter turnip rape (Brassica rapa L.) is widely cultivated in winter in Northwest China, however, its cold-tolerant mechanism remains insufficiently understood. In this study, winter turnip rape cultivar Longyou 7, a cold-tolerant variety, was used as material, whose accumulation of H2O2 and O2•-, antioxidant enzyme activity as well as differences in protein expression based on two-dimensional gel electrophoresis (2-DE) technique under −4°C stress were analyzed. Results showed that, production of H2O2 and O2•- were increased in Longyou 7 leaves, simultaneously, SOD and POD activities were also obviously rosed up, but the activities of CAT and APX were gradually reduced with the temperature. Thirty-six differential protein spots were successfully identified between control and treatments group by using mass spectrometry analysis. Among them, 4 differential protein spots were induced under cold stress, and 2 were inhibited at −4°C. Functional analysis found that these identified proteins mainly participated in photosynthesis, carbohydrate metabolism, defense, protein synthesis, enzyme activity, redox and membrane metabolism, respectively. Additionally, 13 proteins' function were still unknown. In conclusion, strong antioxidant capacity and cell defense ability might play important roles in Longyou 7 response to cold stress.

冬季芜菁油菜(Brassica rapa L.)在西北地区广泛种植,但其耐寒机理尚不清楚。以耐冷芜菁油菜品种龙游7号为材料,利用二维凝胶电泳(2-DE)技术分析了其在- 4℃胁迫下H2O2和O2•-积累、抗氧化酶活性以及蛋白表达的差异。结果表明,龙油7号叶片H2O2和O2•-产量增加,SOD和POD活性明显升高,CAT和APX活性随温度升高而逐渐降低。质谱分析成功鉴定出对照组与处理组之间的36个差异蛋白点。其中,4个差异蛋白斑在冷胁迫下被诱导,2个在−4℃下被抑制。功能分析发现,这些鉴定的蛋白分别主要参与光合作用、碳水化合物代谢、防御、蛋白质合成、酶活性、氧化还原和膜代谢。另外,还有13种蛋白的功能尚不清楚。综上所述,较强的抗氧化能力和细胞防御能力可能在龙油7号对冷胁迫的响应中起重要作用。
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引用次数: 0
A comprehensive review of bioactive compounds and processing technology of sesame seed 芝麻的生物活性成分及其加工技术综述
Q3 Agricultural and Biological Sciences Pub Date : 2022-05-01 DOI: 10.1016/j.ocsci.2022.05.003
Xuan Ma, Zhi Wang, Chang Zheng, Changsheng Liu

Sesame seeds are promulgated as traditional high-quality edible oil crops, rich in lipid (40–65%), protein (19–35%), and bioactive compounds. The review starts with bioactive components (fatty acid, tocopherol, phytosterol, sesamin, sesamolin, and sesamol) of sesame seeds. It considers processing techniques for extracting oil (aqueous extraction and pressing) from seeds. Novel technologies, such as enzyme-assisted aqueous, supercritical CO2, and microwave-assisted solvent extraction, are also discussed. The methods of utilization of sesame seed cake are also analyzed. In the future, the processing technology of sesame seed will be further developed in the direction of improving comprehensive utilization rate to meet new consumption demand.

芝麻是传统的优质食用油料作物,富含油脂(40-65%)、蛋白质(19-35%)和生物活性化合物。从芝麻的生物活性成分(脂肪酸、生育酚、植物甾醇、芝麻素、芝麻素和芝麻醇)开始介绍。它考虑了从种子中提取油(水萃取和压榨)的加工技术。新技术,如酶辅助水,超临界CO2和微波辅助溶剂萃取,也进行了讨论。并对芝麻饼的利用方法进行了分析。未来,芝麻加工技术将朝着提高综合利用率的方向进一步发展,以满足新的消费需求。
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引用次数: 4
Agronomic cultivation measures on productivity of oilseed flax: A review 油籽亚麻生产力的农艺栽培措施综述
Q3 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.1016/j.ocsci.2022.02.006
Zhengjun Cui , Bin Yan , Yuhong Gao , Bing Wu , Yifan Wang , Haidi Wang , Peng Xu , Bangqing Zhao , Zhi Cao , Yong Zhang , Yaping Xie , Yapeng Hu , Xingbang Ma , Junyi Niu

Oilseed flax is one of the most important oil crops in China. With the improvement of people's living standards and the deepening knowledge of the nutritional value of oilseed flax, the demand and economic value of oilseed flax are increasing, and the cultivated area in China is expanding. However, the grain yield of oilseed flax is lower than other oil crops. It varies significantly from year to year, combined with a lower degree of mechanization, which has greatly limited the healthy development of the flax industry. Some of the effects of agronomic measures on productivity and water use efficiency of oilseed flax are reviewed in this paper. The major agronomic strategies for the productivity of oilseed flax were presented based on fertilization, plant density, irrigation, cropping pattern and weed control. Future research should investigate the effect of silicon and potassium fertilizers on the mechanism of lodging resistance of oilseed flax, the effects of diversified cropping systems (strip intercropping and crop rotation) on high and stable productivity and efficient utilization of resources.

油麻是中国最重要的油料作物之一。随着人们生活水平的提高和对油籽亚麻营养价值认识的加深,油籽亚麻的需求和经济价值不断增加,在中国的种植面积不断扩大。然而,油籽亚麻的粮食产量低于其他油料作物。每年变化明显,加上机械化程度较低,极大地限制了亚麻产业的健康发展。本文综述了一些农艺措施对油籽亚麻生产力和水分利用效率的影响。从施肥、密度、灌溉、种植方式和杂草防治等方面提出了提高油籽亚麻生产力的主要农艺策略。今后的研究应进一步探讨钾肥和硅肥对油籽亚麻抗倒伏机理的影响,以及不同种植制度(带状间作和轮作)对高产稳产和资源高效利用的影响。
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引用次数: 12
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