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Epizootological features of cattle leptospirosis in the Vinnitsa region of Ukraine 乌克兰文尼察地区牛钩端螺旋体病的流行病学特征
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-41-48
B. Yarchuk, S. Bilyk, R. Tyrsin, O. Dovgal, P. Shulga, Y. Tyrsina, T. Tsarenko, L. Kornienko, V. Ukhovskyi
Leptospirosis is a common worldwide zoonotic infection, is an important livestock problem throughout Ukraine. The peculiarities of leptospirosis are the presence of more than 250 serological groups of the pathogen, which significantly influences the choice of strategy for prevention and control of this disease.The article presents data on the epizootic situation, the epizootological features of leptospirosis and the etiological structure of leptospiras in cattle in Vinnitsa region (1994-2015) and, in particular, in the farms of the Khmelnitsky district (2006-2012). The epizootic situation with leptospirosis of cattle in Vinnitsa region and the farms of Khmelnytsky district is characterized by certain fluctuations in the number of infections points and sick animals, with relative stability. Tottaly 247 cattle farms infections of leptospirosis were identified in the region during 21 years.The materials for the research were the data of veterinary records and reports of farms and state veterinary medicine establishments of Vinnitsa region, the results of laboratory tests of blood serum from cattle from suspicious farms, the results of the analysis of health and preventive measures in suspicious farms.The main leptospira serogroups that affect cattle in Vinnytsa region and directly in the Khmelnitsky region were Hebdomadis, Sejroe, Icterohaemorrhagiae, Grippotyphosa, Pomona, registered from 43 to 86.3% of mixed reactions.In Khmelnytsky district in 2006-2012, 7 infected farms were registered, 739 animals got sick. It was established that the epizootic situation in the farms depended on the observance of preventive and sanitary measures and the timely introduction of recovery plans. It has been proved that the integrated use of veterinary, sanitary and economic measures is an effective means of eliminating and preventing cattle leptospirosis in livestock farms. The complex of measures should include timely serological analisis of cattle on leptospirosis, adherence to quarantine during importation of animals to the herd, sanitary and preventive measures of exploitation of animals, vaccination of cattle against leptospirosis, taking into account the etiological structure of the disease.Key words: leptospira, leptospirosis, cattle, microscopic agglutination test (MAT), leptospira serological groups, epizootic situation.
钩端螺旋体病是一种常见的世界性人畜共患传染病,是乌克兰境内一个重要的牲畜问题。钩端螺旋体病的特点是存在超过250个病原体血清学组,这显著影响了预防和控制该病策略的选择。本文介绍了文尼察地区(1994-2015年),特别是赫梅利尼茨基地区农场(2006-2012年)牲畜中钩端螺旋体病的流行病学特征和钩端螺旋体的病原学结构的数据。文尼察地区和赫梅利尼茨基地区农场的牛钩端螺旋体病兽疫情况的特点是感染点和患病动物的数量有一定波动,但相对稳定。21年间,该地区共发现247个牛场发生钩端螺旋体病感染。研究的材料是文尼察地区农场和国家兽医机构的兽医记录和报告数据、可疑农场牛的血清实验室检测结果、可疑农场的健康和预防措施分析结果。在Vinnytsa地区和直接在Khmelnitsky地区影响牛的主要钩端螺旋体血清群为Hebdomadis、Sejroe、icterohemorrhagiae、gripo伤寒sa、Pomona,混合反应发生率为43%至86.3%。2006-2012年在赫梅利尼茨基区,登记了7个受感染的农场,739头牲畜患病。已经确定,农场的动物流行病情况取决于是否遵守预防和卫生措施以及及时实施恢复计划。实践证明,综合运用兽医、卫生和经济措施是消除和预防畜牧场牛钩端螺旋体病的有效手段。综合措施应包括及时对牛进行钩端螺旋体病血清学分析,在将动物输入畜群期间遵守检疫,利用动物的卫生和预防措施,在考虑到疾病的病因结构的情况下为牛接种钩端螺旋体病疫苗。关键词:钩端螺旋体,钩端螺旋体病,牛,显微凝集试验(MAT),钩端螺旋体血清学类群,流行情况
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引用次数: 0
Comparative morphofunctional and species-specific vascularization features of of the knee capsule of some mammals 一些哺乳动物膝关节囊的形态功能和种特异性血管化特征的比较
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-57-65
V. Novak, O. Bevz, A. P. Melnichenko
The metabolic, reparative and plastic properties, as well as the functional activity of the multiorgan synovial environment of the knee joint, are organically and functionally related to their morphology and blood supply. The aim of our research was to determine the general biological and specific features of the structural organization and vascularization of the knee joint capsule in animals with different supports, locomotion and body weight. A complex of morphological research methods is used in this work: anatomical, histological, neurohistological. Used neurohistological method of impregnation in its own algorithm. The structural organization of synovial and fibrous capsule, histotopography of intraorganic vessels was studied on 43 objects of various groups of animals. The selection of animals was carried out according to the type of limb specialization to the substrate. Animals were taking into account the ecology, types of support, the nature of locomotion and the speed of movement – phalanoid animals – a domestic horse, a domestic bull and a finger animal – a domestic dog and a domestic cat. As a result of the complex comparative-morphological study the general patterns of the structural organization of the articular capsule, the general-biological and species-specific features of the angioarchitectonics and angiotopography were established. The zones of the most intense intraorganic vascularization and the topography of the vascular fields and glomeruli of the articular capsule are shown. The zones of intensive vascularization are localized: in the domestic bull – in the medial, dorsal, lateral parts, vascular glomeruli – in the lateral; in the domestic horse – in all parts of the capsule, vascular glomeruli – in the plantar area; in the domestic dog – in the medial, dorsal, plantar parts, vascular glomeruli – in the plantar area; in a domestic cat, the same type of angioarchitectonics is observed, the vascular fields and glomeruli are absent. The more intense angioarhitectonics in the capsule of the knee joint in phalanoid animals (horse, cattle) than in the finger (dog, cat) possibly due to the different nature of the support and type of locomotion, is determined.Key words: vascular fields, vascular glomeruli, angioarchitectonics, knee joint, joint capsule, domestic horse, domestic bull, domestic dog, domestic cat.
膝关节多器官滑膜环境的代谢、修复和可塑性特性,以及功能活动,在有机和功能上与它们的形态和血液供应有关。我们研究的目的是确定具有不同支撑、运动和体重的动物膝关节囊结构组织和血管形成的一般生物学和特异性特征。这项工作采用了复杂的形态学研究方法:解剖学、组织学、神经组织学。使用神经组织学方法在自己的算法中浸渍。对43例不同动物标本的滑膜和纤维囊的结构组织、器内血管的组织形态进行了研究。根据基质的肢体特化类型来选择动物。动物们考虑了生态、支撑类型、运动性质和运动速度——指骨动物——一匹家马、一头家牛和一只手指动物——一只家狗和一只家猫。通过复杂的比较形态学研究,确定了关节囊结构组织的一般模式、血管结构学和血管地形图的一般生物学和物种特异性特征。显示了组织内血管最密集的区域以及关节囊的血管区和肾小球的地形图。密集血管形成的区域是局部的:在家养公牛中——在内侧、背侧、外侧,肾小球在外侧;在家马中——在胶囊的所有部分,肾小球——在足底区域;在家犬中——内侧、背侧、足底部分、肾小球——足底区域;在家猫身上,观察到了相同类型的血管结构,没有血管区和肾小球。确定了指骨动物(马、牛)膝关节囊中的血管解剖比手指(狗、猫)更强烈,这可能是由于支撑的性质和运动类型的不同。关键词:血管领域,肾小球,血管建筑学,膝关节,关节囊,家马,家牛,家狗,家猫。
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引用次数: 0
State of protein and mineral exchange in broiler-chickens for the use of Zinc and Mangane chelates 锌和锰螯合物对肉鸡蛋白质和矿物质交换的影响
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-85-94
V. Sakara, A. Melnyk, F. Markhenkov
The results of the application of a complex of Zinc and Manganese chelates to protein, macro- and micro-mineral exchanges in the body of broiler chickens of the Cobb 500 cross-breed are presented. The administration of Zn and Mn chelates in a dose of 0,2 ml/l for chicken broilers of 23 days of age (1st experimental group) during 14 days influenced the increase in the total protein content to 35,3±0,8 g/l, which is 8 % higher compared with the control – 32,5±0,7 g/l (p<0,01 ) The concentration of total Calcium in serum of broiler chickens 37-day-old (end of trial) was higher in the first experimental group by 7,3 % (2,05±0,06 mmol/l) compared to control – 1,9±0,03 mmol/l. In the second group, where chelates were administered in a dose of 0,4 ml/l of water, the Calcium content was 2,0±0,02 mmol/l, which is 5 % more than the control group. The two-week application of Zn and Mn chelates also led to an increase in the level of inorganic Phosphorus in the serum of the first group to 2,6±0,12 mmol/l (+ 11,5 %) compared with the control group. After administration of chelates Zn and Mn at doses of 0,2 ml/l water, the Zinc content was higher by 4,3% (23,5±0,2 μmol/l) compared to control – 22,5±0,34 μmol/l (p<0,01). In the second group, where the dose of chelates was 0,4 ml/l, the concentration of Zinc was 23,6±0,16 μmol/l, which is more than 4,6 % for control (p<0,01). At the end of the study, the content of Manganese in serum of broiler chickens in the first group was 1,9±0,07 μmol/l (p<0,05), which is 10,4% higher compared with the control – 1,7±0,06 μmol/l (3rd sampling). The level of this element in the second group was 2,0±0,08 μmol/l (p<0,01), which is higher by 16,7 % relative to the control. At the end of the experiment, the coupon concentration in the first and second experimental groups was 12,6 and 9,3 % higher, compared with control, 7,0±0,37 μmol/l. In turn, the serum Ferrum content of the first group was greater by 9,3 % (19,3±0,69 μmol/l), and the second by 6,7 % (18,8±0,47 μmol/l) compared to the bird of the control group.Key words: chicken broilers, chelates, Zinc, Manganese, Cuprom, Ferrum, metabolism.
本文介绍了锌锰螯合物在Cobb 500杂交肉鸡体内蛋白质、宏量和微量矿物质交换中的应用结果。政府锌和锰螯合物的剂量的0,2毫升/ l鸡肉鸡23天的年龄(实验1组)14天期间影响总蛋白质含量的增加35岁,3±0,8 g / l,这是与控制- 32相比,高出8% 5±0 7 g / l (p < 0 01)的血清总钙的浓度的肉鸡37-day-old(试验结束)在第一个实验组高7 3%(2,05年±0,06更易/ l)控制- 1相比,9±0,03更易与l。在第二组中,螯合剂以0.4 ml/l的剂量给药,钙含量为2.0±0.02 mmol/l,比对照组高5%。施用锌、锰螯合剂两周后,与对照组相比,第一组血清无机磷水平升高至2.6±0.12 mmol/l(+ 11.5%)。螯合物Zn和Mn以0.2 ml/l的剂量给药后,锌含量比对照组(22.5±0.34 μmol/l)提高了4.3%(23.5±0.2 μmol/l) (p< 0.01)。第二组在螯合剂剂量为0.4 ml/l时,锌浓度为23.6±0.16 μmol/l,高于对照组的4.6% (p< 0.01)。试验结束时,第一组肉鸡血清锰含量为1.9±0.07 μmol/l (p< 0.05),比对照组(第3次取样)1.7±0.06 μmol/l提高了10.4%。第二组中该元素含量为2,0±0,08 μmol/l (p< 0.01),比对照组高16.7%。实验结束时,第一、第二试验组的联剂浓度分别比对照组(7,0±0,37 μmol/l)高12.6、9.3%。与对照组相比,第一组血清铁含量提高了9.3%(19.3±0.69 μmol/l),第二组提高了6.7%(18.8±0.47 μmol/l)。关键词:肉鸡,螯合物,锌,锰,铜,铁,代谢
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引用次数: 1
Peculiarities of acute phase reaction and its correction in surgical pathology in pigs 猪外科病理急性期反应的特点及其纠正
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-111-118
V. Melnikov, M. Rublenko, V. Storozhuk, V. Dudka
Surgical pathology in animals is quite broad and has various forms of manifestation. It covers various anatomic-topographic areas and organs, in particular in pigs quite often presented with abdominal pathology. There have been published reports of an incidence of hernias in 0.8-3.5% of the pig population, which is 50% of all surgical pathology of the whole animal species.In the group of clinically healthy pigs (n = 10), a significant level of anti-inflammatory IL-10 is noticeable. At the same time, rather high cytokine indices of the ratio of anti-inflammatory to proinflammatory cytokines in healthy animals – 13.9:1 – 19.4: 1, with the index between the latter – 0.7: 1. That is, in general, IL-10, under physiological norms, exercises a powerful control over the phylogenic cytokine systems of the pig body.At the same time, animals with surgical pathology show a sharp increase in the levels of proinflammatory cytokines. For hernias accompanied by an adhesive-inflammatory aseptic process, serum TNF-α content is increased 4.9-fold and IL-1β is slightly less – only 2.1-fold, with a moderate increase in the concentration of 1.3-fold anti-inflammatory IL- 10. In this case, cytokine indices are significantly reduced, which indicates a decrease in the activity of antiflogogenic mechanisms.The dynamics of the development of purulent arthritis is characterized by a remarkable increase in blood levels ofTNF-α – 18.3 times, and a moderate increase in the concentrations of IL-1 and IL-10. In this case, the cytokine index ofIL-10: TNF-α acquired a critical value of 1.5: 1, and the number of the latter exceeded the level of IL-1β by more than5 times. At the same time, elevation of IL-1β and IL-10 levels did not lead to a significant difference with the indigenous animals. Most likely, the powerful synthesis of TNF-α is associated with the appearance of a large number of cells of its producers in the area of ​​destruction of the joints. In this case, insufficient increase in the level of IL-10 triggers the process of cascading production of inflammatory mediators and the development of systemic inflammatory syndrome.Fibrinogen, as one of the acute phase reaction proteins, fluctuates within 2–4 g / L by physiological norms. In the early postoperative period, its level increases significantly, however, the peaks of increasing plasma fibrinogen concentration in control animals are recorded on day 1 and day 7. By using the immune depot, on the contrary, it is dynamically normalized to the 7th day after the hernia. The dynamics of fibrinogen concentration with the use of thiotriazoline were somewhat different. It peaked after the first day and was 2.3 times higher than immuno depot injections. In the following, only after the 3rd day the level of fibrinogenemia began to normalize dynamically, which can be explained by the pronounced hepatoprotective effect of thiotriazoline with increased synthesis of fibrinogen by the liver.The level of haptoglobin, like f
动物的外科病理学是相当广泛的,有各种形式的表现。它涵盖了各种解剖地形区域和器官,特别是在猪相当经常出现腹部病理。有已发表的报道称,猪群中有0.8-3.5%的猪发生疝气,占整个动物物种所有外科病理的50%。在临床健康猪组(n = 10)中,抗炎IL-10水平显著升高。同时,健康动物抗炎与促炎因子比值较高,为13.9:1 ~ 19.4:1,促炎与抗炎因子比值介于- 0.7:1之间。也就是说,一般来说,在生理规范下,IL-10对猪体内的系统发育细胞因子系统具有强大的控制作用。与此同时,手术病理动物的促炎细胞因子水平急剧增加。对于伴有黏附-炎症无菌过程的疝,血清TNF-α含量增加4.9倍,IL-1β含量略少,仅增加2.1倍,抗炎IL- 10浓度适度增加1.3倍。在这种情况下,细胞因子指数显著降低,这表明抗泛藻机制的活性降低。化脓性关节炎发展的动力学特征是血液中tnf -α -水平显著升高18.3倍,IL-1和IL-10浓度适度升高。在这种情况下,il -10: TNF-α的细胞因子指数达到了1.5:1的临界值,后者的数量超过IL-1β的5倍以上。与此同时,IL-1β和IL-10水平的升高与本土动物没有显著差异。最有可能的是,TNF-α的强大合成与关节破坏区域中大量细胞的产生有关。在这种情况下,IL-10水平的增加不足触发炎症介质的级联产生过程和全身性炎症综合征的发展。纤维蛋白原作为急性期反应蛋白之一,生理规范在2 ~ 4 g / L范围内波动。在术后早期,其水平显著升高,而对照动物血浆纤维蛋白原浓度升高的峰值出现在第1天和第7天。相反,使用免疫库则动态归一化到疝后第7天。使用硫代三唑啉对纤维蛋白原浓度的影响有所不同。它在第一天后达到峰值,比免疫库注射高2.3倍。随后,仅在第3天之后,纤维蛋白原血症水平开始动态正常化,这可以解释为硫代三唑啉的肝保护作用明显,肝脏纤维蛋白原合成增加。与纤维蛋白原一样,在疝切开术后的最初几个小时内,触珠蛋白的水平几乎保持不变。然而,与术前相比,第一和第二研究组术后24小时其浓度显著增加。第1组的haptoglobin水平是第2组的2倍(p <0.01),说明硫三唑啉的抗炎作用更明显。在术后第3天,该指标出现峰值升高,但在第二个实验组,该指标又降低了2倍。使用硫三唑啉时,血清触珠蛋白浓度的进一步动态下降更为明显。手术后第6小时,第1组猪血清铜蓝蛋白含量达到最高,为421.9±39.69 mg / l。血清铜蓝蛋白的快速升高很可能是由于免疫库成分(尤其是硒和铜)抑制了促氧化酶。然而,鉴于组内铜蓝蛋白浓度的既定动态,不可能证明其对猪无菌性炎症的特异性。手术病理猪的细胞因子谱取决于其复杂性。中度细胞素血症的发生性发展在疝,这变得非常明显与化脓性关节炎。然而,在猪的手术病理中,抗炎细胞因子的产生不足以需要药物纠正炎症反应。猪疝切开术后急性期反应主要表现为血液中纤维蛋白原和触珠蛋白浓度升高,而铜蓝蛋白不升高。硫代三唑啉和免疫库对其纠正机制存在差异,有待进一步研究。关键词:猪,细胞因子,急性期蛋白,疝切开术,免疫库,噻唑啉。
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引用次数: 1
Influence of microbiological starter of filler of biofilter of "Filtronorm D" on stored of rainbow trout “滤池D”生物滤池填料微生物发酵剂对虹鳟贮藏的影响
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-15-21
N. Grynevych
Creation in biofilters of favorable conditions for the existence of biocenoses secures RAS from the toxic effects of nitrites, which until recently did not attach great importance to toxicants for aquatic organisms. However, it has been found that they are very toxic to fish and aquatic invertebrates. The purpose of the work was to investigate the effect of microbiological starter of the "Filtronorm-D" biofilter filler on the behavior of adult rainbow trout during the launch of the biofilter.Investigation of the influence of the microbial starter of the filtrotorm-D biofilter filler on the behavior and clinical condition and the preservation of rainbow trout during the launch of the biofilter of the ultrasound has been studied.Evaluated the clinical signs of adult rainbow trout during the launch of the biofilter CWS for the use of polypropylene filler RK PLAST-control in it. According to the second variant, the clinical signs of adult rainbow trout were evaluated during the startup of the biofilter of the ultrasound scanner for the use of polypropylene filler RK PLAST in it and the addition of the microbiological starter of the biofilter "Filtronorm-D" – experiment. In the experimental group, the biofilter water was introduced by our microbiological starter, biofilter filler "Filtronorm-D", containing live cultures of nitrifying and denitrifying bacteria in the amount of 107 CFU/cm3.On the 10th day of the experiment, after the application of the microbiological starter of the biofilter filler "Filtronorm-D",approximately 5 % of the fish with the initial clinical signs of nitrite poisoning were detected. The fish for a long time stood motionless in the corners of the pool, they had the darkening of the body and the brown color of the gills. On the 15th day after the launch of the CWS and the use of "Filtronorm-D", changes in behavior were detected in most of the research objects, which may indicate an increase in the amount of nitrites in water and their effect on the body of trout. Approximately 25 % of fish for a long time stays motionless in the corners of the pool, the mobility of the whole fish has decreased, some individuals have risen to the surface of the water. Changes in pigmentation of the body were also found in approximately10 % of the individuals. The body became darker, the fins were sealed, the gills got brown color. During this trial period, the mortality rate of the fish was 1,3 %. On the 20th day of the experiment, the signs of nitrite poisoning of fish became increasingly pronounced. Approximately 30 % of the fish showed the presence of darkening of the body and fins, the latter were sealed. In the same fish, the changed color of the gills was observed – from red to brown. In addition, during this period of research, the highest trout death was determined – 3,1 %, which is almost 2.4 times (p <0,05) higher than in the 15-day period. The period of ultrasound starting from the 20th to the 25th day was characterized by a decr
在生物过滤器中创造有利于生物群落存在的条件,确保RAS免受亚硝酸盐的毒性作用,直到最近才对水生生物的有毒物质给予高度重视。然而,人们发现它们对鱼类和水生无脊椎动物有很大的毒性。本研究的目的是研究“Filtronorm-D”生物滤池填料的微生物发酵剂对虹鳟鱼成鱼在生物滤池启动过程中行为的影响。研究了filtroform - d型生物滤池填料的微生物发酵剂对虹鳟鱼在超声生物滤池启动过程中的行为、临床状况及保存的影响。评价聚丙烯填料RK - PLAST-control在虹鳟鱼生物过滤器CWS投放过程中成鱼的临床表现。根据第二种变体,在超声扫描仪的生物滤池中使用聚丙烯填料RK PLAST,并加入生物滤池“Filtronorm-D”实验的微生物发酵剂,对虹鳟鱼成虫的临床症状进行评估。试验组生物滤水采用我们的微生物发酵剂——生物滤池填料“Filtronorm-D”,其中含有107 CFU/cm3的硝化和反硝化菌活培养物。实验第10天,在使用生物过滤填料“Filtronorm-D”的微生物发酵剂后,检测到约5%的鱼出现亚硝酸盐中毒的初步临床症状。这些鱼在池塘的角落里一动不动地站了很长时间,它们的身体变黑了,腮变成了棕色。在CWS启动并使用“滤镜- d”后的第15天,大多数研究对象的行为发生了变化,这可能表明水中亚硝酸盐的含量增加及其对鳟鱼身体的影响。大约25%的鱼长时间呆在池塘的角落里不动,整个鱼的机动性下降,一些个体已经上升到水面。在大约10%的个体中也发现了身体色素沉着的变化。身体变黑了,鳍被封住了,腮变成了棕色。试验期间,鱼的死亡率为1.3%。在实验的第20天,鱼类亚硝酸盐中毒的迹象越来越明显。大约30%的鱼显示出身体和鳍变黑的存在,后者是密封的。在同一条鱼身上,观察到鱼鳃的颜色从红色变成了棕色。此外,在研究期间,鳟鱼的最高死亡率为3.1%,几乎是15天期间的2.4倍(p < 0.05)。从第20天到第25天的超声期间,亚硝酸盐含量从1.1 mg/dm3下降到0.6 mg/dm3。降低水中亚硝酸盐含量的过程影响了鱼类的活动-只有大约20%的鳟鱼在上层水中游泳。在使用生物过滤填料“Filtronorm-D”的微生物发酵剂的超声波启动的第30天,虹鳟鱼中没有发现亚硝酸盐中毒的迹象。在指定期间,鱼的损失实际上没有标明。同时,在使用聚丙烯填料RK PLAST而不添加生物过滤填料“Filtronorm D”的微生物发酵剂期间,鱼类最成问题的时期是25 - 30天,这一时期由于亚硝酸盐的影响,鱼类最容易死亡。因此,所进行的研究证明,在超声波发射过程中使用聚丙烯填料RK PLAST并在其中添加微生物发酵剂的生物滤池“Filtertronorm D”滤池最危险的时期是15 ~ 20天。在此期间,鱼类死亡人数最多,亚硝酸盐中毒的迹象也最多,因此需要采取兽医和卫生预防措施,以减少亚硝酸盐的毒性作用。关键词:RAS,虹鳟鱼,生物过滤器,滤镜- d,亚硝酸盐,鱼类行为,中毒迹象
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引用次数: 0
Analysis of the insemination of the mesophilic and psychrotrophic microflora of frozen fish 冻鱼中嗜温和嗜冷菌群的授精分析
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-22-29
Z. Malimon, M. Kukhtyn, N. Grynevych, N. Mekh
The article presents the results of research on the dehiscence of frozen fish with mesophilic and psychrotrophic microflora. Physico-chemical and organoleptic changes which appear in fish during refrigeration are connected with the life of the psychotropic group of microflora, which is more active than mesophilic. Fish are a nutrient medium for the development of microorganisms of all groups, due to its high nutritional and biological value, so the fish are perishable food products, the conditions and terms of their storage require appropriate temperature regimes to stop the development of microorganisms. The aim of the work was carrying out a comparative analysis of insemination of frozen fish with mesophilic and psychrotrophic microflora to make an amend to standards according to microbiological criteria.The microbial number in frozen fish samples was estimated with the temperature of (30 ± 1) ºC incubation of crops for 72 hours (mesophilic microflora) and incubation for 10 days (psychrotrophic microflora) with the temperature (6.5 ± 0.5) ºC.It was identified that there were taken the samples from frozen fish with a quantity of mesophilic bacteria to 102 CFU/g, 1.4-1.8 times (p <0.05) more psychotropic microorganisms. The researched samples with the number of mesophilic microorganisms from 103 to 104 CFU/g were contaminated with psychotropic microflora, which in 1.7-6.8 times (p <0.05) exceeded the content of the mesophilic microflora. With such amount of mesophilic microorganisms, on average of up to 25% of samples, this had a content of psychotrophs of more than 105 CFU/g of fish. According to the content of mesophilic bacteria the samples of frozen fish, which were mathed to a certain norm of 5 × 104 CFU/g, basically in the number of psychrotrophic microflora did not correspond to this indicator, and exceeded it 2 times or more.In the cold period of the year, 63.6 ± 2.1% of frozen fish samples were mesophilic bacteria containing less than 101 CFU/g. At the same time, samples with such content mesophilic bacteria in the warm period of the year was 9.0%, or 7.0 times(p <0.05) less. In addition, in the cold period of the year, only 9.0% of samples were detected, which, according to the content of mesophilic bacteria exceeded the maximum allowable level. At the same time, during the warm period, the number of samples with an excess of mesophilic bacteria content was 27.3 ± 0.3%. Practically the same pattern was observed regarding the insemination of the psychrotrophic microflora in these periods of the year, which was characterized by the fact that in the warm period of the year, frozen fish contains a large number of psychrotrophic microorganisms. Consequently, the results of studies on the amount of microflora in the warm period of the year established 3,0 times (p <0,05) more samples of frozen fish, which, according to the content of mesophilic bacteria, exceeded the maximum permissible level compared with the cold period of the year.It
本文介绍了利用嗜温和嗜冷菌群对冷冻鱼开裂的研究结果。鱼类在冷藏过程中出现的物理化学和感官变化与精神菌群的生命有关,精神菌群比中温菌群更活跃。鱼类是所有类群微生物发育的营养介质,由于其具有很高的营养和生物学价值,因此鱼类是易腐烂的食品,其储存条件和条件需要适当的温度制度来阻止微生物的发育。这项工作的目的是对冷冻鱼中嗜温菌群和嗜冷菌群的授精进行比较分析,以便根据微生物学标准修订标准。在(30±1)℃的温度下,作物培养72小时(中温菌群),在(6.5±0.5)℃的温度下培养10天(嗜冷菌群),估计冷冻鱼样品中的微生物数量。结果表明,冻鱼样品中嗜中温菌含量达到102 CFU/g,是精神类微生物的1.4 ~ 1.8倍(p <0.05)。嗜温菌数在103 ~ 104 CFU/g的研究样品中,精神菌群的污染程度是嗜温菌群的1.7 ~ 6.8倍(p <0.05)。平均高达25%的样品中含有如此多的中温微生物,这意味着每克鱼的精神营养物含量超过105 CFU。根据冻鱼样品中嗜酸性细菌的含量,计算出5 × 104 CFU/g的一定标准,结果显示,冻鱼样品中嗜酸性微生物的数量基本不符合该指标,甚至超过该指标2倍以上。在一年中寒冷时期,63.6±2.1%的冷冻鱼样品为中温细菌,含量低于101 CFU/g。与此同时,暖季中温细菌含量的样品为9.0%,比暖季低7.0倍(p <0.05)。此外,在一年中的寒冷时期,只有9.0%的样品被检出,根据中温细菌的含量,超过了最大允许水平。同时,在温暖期,嗜温菌含量超标的样品数量为27.3±0.3%。实际上,在一年中的这些时期,在精神营养菌群的授精方面观察到相同的模式,其特点是在一年中的温暖时期,冷冻鱼含有大量的精神营养微生物。因此,对冻鱼样品的微生物菌群数量的研究结果表明,暖期冻鱼样品的微生物菌群数量是寒期冻鱼样品的3 000倍(p < 0.05),根据中温细菌的含量,冻鱼样品的微生物菌群数量超过了冷期冻鱼样品的最大允许水平。结果表明,含中温微生物小于101 CFU/g的冷冻鱼样品受嗜冷菌群污染最不均匀。其中,只有30.1±1.4%的样品的精神营养菌群数量小于101 CFU/g, 60.0±0.5%的样品的精神营养菌群数量在101 ~ 105 CFU/g之间,10.0±0.2%的样品的精神营养菌群数量大于105 CFU/g。在对101 ~ 102 CFU/g的中温细菌数量的冷冻鱼样品的研究中,只有16.7±0.3%的样品与102 ~ 103 CFU/g的嗜冷菌群含量一致,33.3±0.3%的鱼样品与102 ~ 103 CFU/g的嗜冷菌群含量一致,103 ~ 104 CFU/g和16.7±0.3%的鱼样品被超过104 CFU/g的嗜冷菌污染。结果表明,含中温微生物小于101 CFU/g的冷冻鱼样品受嗜冷菌群污染最不均匀。其中,只有30.1±1.4%的样品的精神营养菌群数量小于101 CFU/g, 60.0±0.5%的样品的精神营养菌群数量在101 ~ 105 CFU/g之间,10.0±0.2%的样品的精神营养菌群数量大于105 CFU/g。在对101 ~ 102 CFU/g的中温细菌数量的冷冻鱼样品的研究中,只有16.7±0.3%的样品的嗜冷菌含量与102 ~ 103 CFU/g的嗜冷菌群含量相符,33.3±0.3%的鱼样品与嗜冷菌群含量相符,103 ~ 104 CFU/g和16.7±0.3%的鱼样品的嗜冷菌群含量超过104 CFU/g。结果表明,冷冻鱼的嗜冷菌群在数量上占主导地位,嗜温菌数个数量级。 在温暖季节,检出的冻鱼样品较多,有3 000次(p < 0.05),从中温细菌的含量来看,与寒冷季节相比,超过了允许的最大水平。结果发现,92,6±2.5%的冷冻鱼样品符合DSTU 4868: 2007的要求。鱼是冷冻的。同时,在鱼类评价过程中,嗜冷菌群的含量显示,超过5 × 104 CFU/g的样品是中温菌含量的2.6倍(p <0.05)。今后将对冷冻鱼的嗜冷菌群的属和种组成进行研究,并根据嗜冷菌对鱼类的评价标准进行研究,以便根据微生物学标准进行修正。关键词:冷冻鱼,嗜冷菌群,中温细菌,污染,微生物数量
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引用次数: 0
INTERNATIONAL AND NATIONAL LEGISLATION TO CONTROL MICTOXINS IN FOOD: REVIEW 控制食品中微毒素的国际和国家立法:审查
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-30-40
O. Khitska, R. Gerard
Today, the problem of monitoring mycotoxins has become global in connection with climate change, a violation of the ecological balance for the use of intensive technologies for processing crops, through air pollution and the accumulation of products of photochemical reactions (photooxidants), which leads to a decrease in plant resistance to phytopathogens.Every year, the problem of mycotoxicosis is exacerbated, as toxic fungi adapt quickly to new technologies and modern plant protection products. The increase in mycotoxins in foods also relates to the widespread use of nitrogen fertilizers and pesticides.Natural toxins create risks for the health of humans and animals, affect food security and nutrition, reducing people's access to healthy food. The World Health Organization is constantly appealing to national authorities to monitor and ensure that the levels of the most relevant natural toxins in foods are as low as possible and consistent with both national and international requirements.Ukraine's membership in the WTO, an association with the European Union, and the expansion of international trade require solutions to the issues of free movement of goods, safe and healthy food, and, accordingly, an adequate level of protection of life and health of people. One of the most important ways to solve them is to improve and harmonize national food legislation in line with international standards, including on the control of mycotoxins.The purpose of our work was to conduct an analysis of literary sources, international and national legislative acts on the control of mycotoxins in food products throughout the food chain.To prepare the publication, we have used literary sources on the subject of publication, as well as we have conducted a comparative analysis of national and international legislative acts regulating procedures and methods for controlling the residues of mycotoxins in food.An analysis of numerous sources has shown that the issue of monitoring mycotoxins in foods, improving laboratory control and risk-based approach to preventing foodborne mycotoxicosis worries scientists from different countries, including Ukrainian. The analysis of national legislation shows that national standards on maximum levels of pollutants have been revised in Ukraine and a number of standards have been harmonized for methods of monitoring the residues of mycotoxins in feed for productive animals, food products of animal and vegetable origin.Key words: mycotoxins, food chain, food, international law, national legislation, control, safety, risks.
今天,监测真菌毒素的问题已成为与气候变化有关的全球性问题,使用集约化技术加工作物违反了生态平衡,空气污染和光化学反应产物(光氧化剂)的积累导致植物对植物病原体的抗性下降。随着有毒真菌对新技术和现代植物保护产品的快速适应,真菌中毒问题每年都在加剧。食品中真菌毒素的增加还与氮肥和农药的广泛使用有关。天然毒素对人类和动物的健康造成风险,影响粮食安全和营养,减少人们获得健康食品的机会。世界卫生组织不断呼吁各国当局监测并确保食品中最相关的天然毒素含量尽可能低,并符合国家和国际要求。乌克兰加入世贸组织、加入欧洲联盟以及扩大国际贸易,都需要解决货物自由流动、安全和健康食品的问题,并相应地适当保护人民的生命和健康。解决这些问题的最重要方法之一是根据国际标准,包括真菌毒素控制标准,改进和协调国家食品立法。我们工作的目的是对整个食品链中食品中真菌毒素控制的文献来源、国际和国家立法行为进行分析。为了准备该出版物,我们使用了有关出版主题的文献资料,并对规范控制食品中真菌毒素残留的程序和方法的国家和国际立法行为进行了比较分析。对众多来源的分析表明,监测食品中的真菌毒素、改善实验室控制和以风险为基础的方法来预防食源性真菌中毒的问题令包括乌克兰在内的不同国家的科学家感到担忧。对国家立法的分析表明,乌克兰修订了关于污染物最高水平的国家标准,并统一了一些标准,用于监测生产动物饲料、动物和蔬菜来源的食品中真菌毒素残留的方法。关键词:真菌毒素,食物链,食品,国际法,国家立法,控制,安全,风险。
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引用次数: 0
THE EFFECTIVENESS OF A COMPREHENSIVE TREATMENT REGIMEN USING SODIUM THIOSULFATE WITH GASTROINTESTINAL DISEASES IN CALVES WITH INTOXICATION SYNDROME 用硫代硫酸钠治疗中毒综合征犊牛胃肠道疾病的综合治疗方案的有效性
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-95-101
A. Belko, M. Matsinovich, V. Petrov, A. A. Matsinovich
The aim of the research was to study the dependence of the severity on the degree of endogenous intoxication and the effectiveness of complex treatment of calves using sodium thiosulfate-based drugs for gastrointestinal diseases. Studies were carried out under production conditions in cattle farms of the Vitebsk, Liozno and Gorodok districts. As preparations based on sodium thiosulfate, the veterinary drug "Averon" produced by LLC Belekotekhnika RB and the veterinary preparation Antitox manufactured by ImmCont GmbH, Germany, were used. These drugs as an antitoxic agent, were administered once a day, intravenously, slowly at a dose of 25 ml, until the clinical signs of the disease disappeared in addition to the basic treatment taken in the farms with gastrointestinal diseases.The data obtained as a result of the analysis of the literature and the conduct of our own research showed that in the pathogenesis of gastrointestinal diseases of the calves, the link to the pathogenesis, which significantly determines the severity of their course and prognosis, is endogenous intoxication. It was found that in patients with calves, the MW content was higher at the height of the disease – 1.75–2 times with moderate severity and 1.94 and 2.47 times with severe. Laboratory criteria were established for predicting the severity of dyspepsia and abomasoenteritis in calves of 14 to 30 days of age. So, at a level higher than 0,120 conv. units in 100% of such calves, dyspepsia or gastroenteritis proceeded in a severe (toxic) form. The significant direct correlations between the concentration of MWS and LPO products (r ≥ 0.650) revealed at the height of the disease indicate the significance of LPO as a source in the development of endotoxication in gastrointestinal diseases in calves. It has been established that the use of drugs with antitoxic action based on sodium thiosulphate in the complex therapy of calves of patients with dyspepsia and abomasoenteritis can reduce the severity and lethality of calves with dyspepsia by 5% to 10%.Key words: calves, blood, endogenous intoxication, abomazoenteritis, dyspepsia, therapeutic efficacy, treatment.
本研究的目的是研究犊牛内源性中毒程度对其严重程度的依赖性以及以硫代硫酸钠为基础的胃肠道疾病药物综合治疗的有效性。在维捷布斯克、利奥兹诺和戈罗多克地区养牛场的生产条件下进行了研究。作为基于硫代硫酸钠的制剂,使用LLC Belekotekhnika RB生产的兽药“Averon”和德国imcont GmbH生产的兽用制剂Antitox。这些药物作为一种抗毒剂,每天静脉注射一次,剂量为25毫升,缓慢服用,直到在患有胃肠疾病的农场采取的基本治疗之外,疾病的临床症状消失。通过文献分析和我们自己的研究获得的数据表明,在犊牛胃肠道疾病的发病机制中,内源性中毒是致病机制的一个环节,它显著地决定了犊牛病程和预后的严重程度。结果发现,在小牛犊患者中,疾病高度时MW含量较高,中度患者为1.75-2倍,重度患者为1.94 - 2.47倍。建立了预测14 ~ 30日龄犊牛消化不良和皱胃肠炎严重程度的实验室标准。因此,在100%的犊牛中,高于0,120个单位的水平,消化不良或肠胃炎以严重的(有毒的)形式进行。MWS和LPO产物浓度在疾病高峰期呈显著的直接相关(r≥0.650),这表明LPO在犊牛胃肠道疾病的内毒素发展中具有重要意义。已有研究证实,在犊牛消化不良合并皱胃肠炎患者的综合治疗中,使用以硫代硫酸钠为基础的抗毒药物,可使犊牛消化不良的严重程度和死亡率降低5% ~ 10%。关键词:小牛,血液,内源性中毒,厌氮肠炎,消化不良,疗效,治疗。
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引用次数: 0
The indexes of mineral exchange in laying hens under the influence of Selenium and Zinc nonoacquahelates and vitamin E 非水乳酸盐硒、锌和维生素E对蛋鸡矿物质交换指标的影响
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-49-56
M. Nischemenko, V. Kaplunenko, V. Koziy, O. Poroshinska, L. Stovbetska, A. Yemelyanenko, O. Omelchuk
The article presents the results of the study of the influence of nanoacquahelats of biogenic and biocidal metals Selenium and Zinc together with vitamin E, on the mineral metabolism indexes of hens of Loman Brown breed. It is known that the inflow of mineral elements such as Calcium and inorganic Phosphorus into the body and its proper usage is essential for the maintenance of egg productivity and egg quality. In this regard, it is promising to study the changes of the above-mentioned processes with the Selenium, Zinc and vitamin E nanoacqualates supplementation. It has been established that the feeding of Selenium and Zinc nanocarboxylic acids with vitamin E positively affects the exchange of Calcium and inorganic Phosphorus in the laying hens. In particular, the content of calcium in the serum of tested hens was significantly higher (17.3-26.7%), on the 60th and 90th days of the experiment, comparing with the control group. The level of inorganic phosphorus, on the 60th and 90th days of the experiment, also increased by 17.7% and 16.9%, respectively, in experimental group of layer hens compared to control one.It is known that macroelements Ca and inorganic Phosphorus are important components for the organism of animals and, especially, birds. These minerals have no nutritional value, are not used by the body as a source of energy, but their role as structural elements cannot be overestimated. Both Calcium and inorganic Phosphorus are part of almost all organs and tissues. The growth and development of the organism, as well as its productivity depends on the presence of these elements in the diet. In addition, Calcium and inorganic Phosphorus are actively involved in the regulation of a number of vital functions. In the birds, it is the synthesis of proteins and the intense formation of egg minerals. They are also necessary for the further formation of bone tissue of the embryo. Consequently, the presence of these minerals in the diet and in the body of layers hens, to a large extent determine their weight and quality of eggs.The obtained results show the use of nanoacquahelats of Selenium and Zinc together with vitamin E stimulate phosphorus-calcium metabolism and alkaline phosphatase activity due to their biophysical properties which are based on activation of many biochemical processes according to the effect of Borisevich-Kaplunenko-Kosinov.Key words: Calcium, Phosphorus, laying hens, Selenium, Zinc, vitamin E.
本文介绍了生物源性和生物杀灭性金属硒、锌纳米复合剂与维生素E对洛曼布朗种鸡矿物质代谢指标影响的研究结果。众所周知,钙和无机磷等矿物元素流入体内并正确使用,对维持鸡蛋产量和鸡蛋品质至关重要。因此,研究添加硒、锌和维生素E纳米藻酸盐对上述过程的影响是有希望的。综上所述,饲粮中添加硒、锌纳米羧酸与维生素E对蛋鸡钙和无机磷的交换有积极影响。特别是试验第60和90天,试验鸡血清钙含量显著高于对照组(17.3 ~ 26.7%)。试验第60天和第90天,试验组蛋鸡无机磷水平也分别比对照组提高了17.7%和16.9%。众所周知,大量元素钙和无机磷是动物,特别是鸟类的重要组成部分。这些矿物质没有营养价值,不被身体用作能量来源,但它们作为结构元素的作用不容小觑。钙和无机磷几乎是所有器官和组织的组成部分。有机体的生长发育及其生产力取决于饮食中这些元素的存在。此外,钙和无机磷还积极参与许多重要功能的调节。在鸟类中,它是蛋白质的合成和蛋矿物质的强烈形成。它们也是胚胎骨组织进一步形成所必需的。因此,这些矿物质在蛋鸡的饮食和身体中的存在,在很大程度上决定了它们的体重和鸡蛋的质量。结果表明,硒、锌纳米复合剂与维生素E联合使用可刺激磷钙代谢和碱性磷酸酶活性,这是由于它们的生物物理特性,根据Borisevich-Kaplunenko-Kosinov的作用,它们可以激活许多生化过程。关键词:钙、磷、蛋鸡、硒、锌、维生素E。
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引用次数: 2
Comparative effectiveness of complex treatment schemes for rabbits with pasalurosis and psoroptosis 复合治疗方案对兔pasaluosis和psordiosis的疗效比较
Pub Date : 2019-05-30 DOI: 10.33245/2310-4902-2019-149-1-66-74
D. Feshchenko, O. Zghozinska, O. Dubova, T. Bakhur, V. Goncharenko, Yu. Stoliarova
Psoroptosis and pasalurosis in rabbits for their maintenance in unsanitary conditions often becomes massive. To increase the effectiveness of treatment of rabbits it is expedient to use combined drugs based on active substances with different mechanisms of action on parasites. Rabbits of the first experimental group applied spray treatment with acaricidal properties of kerosene + glycerol (1:1) three times with an interval of 5 days. Spraying the inner surface of the ears was carried out after mechanical cleaning with gauze swabs soaked in iodine solution + glycerin (1:4). To destroy the helminths, brovadasol plus (5 g/10 kg body weight), orally with food, was administered once. For animals of the second experimental group, the "brovermectin 2%" helminthoacaricide was chosen, it was orally administered at a dose of 1 ml/50 kg body weight, divided into 5 days of regular use. Mechanical treatment of ear with chlorhexidine bigluconate was carried out analogously to rabbits of the first group twice a day for 10 days. On the day of treatment with the rabbits in the second group, Dexacel 0.2 % (KELAN.V., Belgium) was injected intramuscularly with a dose of 0.1/1 kg of body weight, in order to improve the general animal's stanchion.For combined infestations (scabies and nematodes) it is advisable to use preparations based on ivermectin (in our study, "Brovermectin 2%" at a dose of 1 ml/50 kg, which was divided into 5 parts and daily diluted one part in 1/2 of the daily water) In the case of the need to treat only psoroptosis, one can use a kerosene solution in glycerol (1:1) in the form of a spray.A prerequisite for the effective treatment of psoropathy is the regular mechanical cleaning of the affected ears from the purulent parasitic exudate or iodine solution + glycerol (1:4) or Chlorhexidine bigluconate 0,05%. It is appropriate to use "Dexacel 02" (one time, non-inflammatory y dose of 0.1/1 kg body weight) in order to improve the general animal's condition.The use of "Brovadazole plus" (once, with food, at a dose of 5 g/10 kg body weight) did not ensure 100% destruction of Passalurus ambiquus, but due to the short carencia period (only 7 days), if necessary, can be applied to fattening rabbits in the last 2 weeks before slaughter. In contrast to the period of carencia, "Brovermectin 2%" is at least 24 days.Key words: rabbit, psoroptosis, pasalurosis, brovermectin, acaricidal efficacy, Dexacel 02.
家兔在不卫生的环境中饲养时,经常出现大量的腰光症和腰光症。为了提高家兔的治疗效果,使用基于不同作用机制的活性物质的联合药物是有利的。第一实验组家兔喷施具有杀螨性能的煤油+甘油(1:1)喷施3次,间隔5 d。用纱布拭子浸泡碘溶液+甘油(1:4)进行机械清洗后喷涂耳内表面。为了杀灭蠕虫,给药1次加布伐达索(5 g/10 kg体重),随食物口服。第二实验组选用“2%布维菌素”杀螨剂,按1 ml/50 kg体重口服,分5天定期使用。对第一组家兔进行双酚酸氯己定耳部机械治疗,每日2次,连用10 d。第二组家兔治疗当日,右赛赛0.2% (KELAN.V.)(比利时)以0.1 kg体重的剂量肌肉注射,以改善一般动物的支柱。对于合并感染(疥疮和线虫),建议使用基于伊维菌素的制剂(在我们的研究中,“2%布维菌素”的剂量为1ml / 50kg,分为5份,每天用每日1/2的水稀释1份)。在需要仅治疗吸光症的情况下,可以使用甘油(1:1)煤油溶液喷雾。有效治疗银屑病的先决条件是定期对受影响的耳朵进行机械清洁,清除化脓性寄生虫渗出液或碘溶液+甘油(1:4)或氯己定(0.05%)。为了改善一般动物的状况,宜使用“Dexacel 02”(一次性,无炎症剂量0.1/1 kg体重)。“布洛瓦唑+”(一次,与食物一起,剂量为5克/10公斤体重)并不能保证100%杀灭双曲曲鼠,但由于放瘤期短(仅7天),如有必要,可在屠宰前最后2周对育肥兔施用。与卡伦西亚相比,“2%布洛维菌素”至少需要24天。关键词:兔子,吸光症,pasalurosis, brovermectin,杀螨效果,Dexacel 02。
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引用次数: 0
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Naukovii visnik veterinarnoyi meditsini
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