首页 > 最新文献

Current Research in Microbial Sciences最新文献

英文 中文
Clostridium tetani bacteraemia in the plague area in France: Two cases
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100339
M.A. Boualam , A. Bouri , M. Signoli , M. Drancourt , A. Caputo , E. Terrer , G. Aboudharam
Clostridium tetani (C. tetani) bacteraemia is a rare situation, with only four case reports in the literature. Fourteen teeth from the 1590 plague site in Fédons, France, were surface decontaminated before the pulp was cultured under strict anaerobiosis with negative controls. Colonies were identified by mass spectrometry and whole genome sequencing, and C. tetani-specific PCR was performed using DNA extracted from dental pulps, calculus and sediments. C. tetani cultured in two dental pulp specimens from two individuals was firmly identified by MALDI-TOF mass spectrometry, and whole genome sequencing confirmed toxigenic C. tetani. In the remaining twelve individuals, no such C. tetani was recovered and further detection by PCR and palaeoculturomics of dental calculus and sediments surrounding the teeth in these two individuals remained negative. Toxigenic C. tetani which did not result from mere environmental contamination, caused bacteraemia in two individuals from a modern time plague site in France.
{"title":"Clostridium tetani bacteraemia in the plague area in France: Two cases","authors":"M.A. Boualam ,&nbsp;A. Bouri ,&nbsp;M. Signoli ,&nbsp;M. Drancourt ,&nbsp;A. Caputo ,&nbsp;E. Terrer ,&nbsp;G. Aboudharam","doi":"10.1016/j.crmicr.2025.100339","DOIUrl":"10.1016/j.crmicr.2025.100339","url":null,"abstract":"<div><div><em>Clostridium tetani</em> (<em>C. tetani</em>) bacteraemia is a rare situation, with only four case reports in the literature. Fourteen teeth from the 1590 plague site in Fédons, France, were surface decontaminated before the pulp was cultured under strict anaerobiosis with negative controls. Colonies were identified by mass spectrometry and whole genome sequencing, and <em>C. tetani-</em>specific PCR was performed using DNA extracted from dental pulps, calculus and sediments. <em>C. tetani</em> cultured in two dental pulp specimens from two individuals was firmly identified by MALDI-TOF mass spectrometry, and whole genome sequencing confirmed toxigenic <em>C. tetani</em>. In the remaining twelve individuals, no such <em>C. tetani</em> was recovered and further detection by PCR and palaeoculturomics of dental calculus and sediments surrounding the teeth in these two individuals remained negative. Toxigenic <em>C. tetani</em> which did not result from mere environmental contamination, caused bacteraemia in two individuals from a modern time plague site in France.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100339"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multifaceted virus-like particles: Navigating towards broadly effective influenza A virus vaccines 多面病毒样颗粒:走向广泛有效的甲型流感病毒疫苗。
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100317
Jaffar Ali Muhamad Norizwan, Wen Siang Tan
The threat of influenza A virus (IAV) remains an annual health concern, as almost 500,000 people die each year due to the seasonal flu. Current flu vaccines are highly dependent on embryonated chicken eggs for production, which is time consuming and costly. These vaccines only confer moderate protections in elderly people, and they lack cross-protectivity; thereby requiring annual reformulation to ensure effectiveness against contemporary circulating strains. To address current limitations, new strategies are being sought, with great emphasis given on exploiting IAV's conserved antigens for vaccine development, and by using different vaccine technologies to enhance immunogenicity and expedite vaccine production. Among these technologies, there are growing pre-clinical and clinical studies involving virus-like particles (VLPs), as they are capable to display multiple conserved IAV antigens and augment their immune responses. In this review, we outline recent findings involving broadly effective IAV antigens and strategies to display these antigens on VLPs. Current production systems for IAV VLP vaccines are comprehensively reviewed. Pain-free methods for administration of IAV VLP vaccines through intranasal and transdermal routes, as well as the mechanisms in stimulating immune responses are discussed in detail. The future perspectives of VLPs in IAV vaccine development are discussed, particularly concerning their potentials in overcoming current immunological limitations of IAV vaccines, and their inherent advantages in exploring intranasal vaccination studies. We also propose avenues to expedite VLP vaccine production, as we envision that there will be more clinical trials involving IAV VLP vaccines, leading to commercialization of these vaccines in the near future.
甲型流感病毒(IAV)的威胁仍然是一个年度健康问题,因为每年有近50万人死于季节性流感。目前的流感疫苗高度依赖于胚胎鸡蛋的生产,这既耗时又昂贵。这些疫苗仅对老年人提供适度保护,而且缺乏交叉保护;因此需要每年重新配方,以确保对当代流行菌株的有效性。为了解决目前的限制,正在寻求新的战略,重点是利用禽流感病毒的保守抗原开发疫苗,并通过使用不同的疫苗技术来增强免疫原性和加快疫苗生产。在这些技术中,涉及病毒样颗粒(vlp)的临床前和临床研究越来越多,因为它们能够显示多种保守的IAV抗原并增强其免疫反应。在这篇综述中,我们概述了有关广泛有效的IAV抗原和在VLPs上展示这些抗原的策略的最新发现。全面审查了目前IAV VLP疫苗的生产系统。本文详细讨论了经鼻和经皮途径给药IAV VLP疫苗的无痛方法,以及刺激免疫反应的机制。讨论了VLPs在IAV疫苗开发中的未来前景,特别是它们在克服当前IAV疫苗免疫局限性方面的潜力,以及它们在探索鼻内疫苗接种研究方面的固有优势。我们还提出了加快VLP疫苗生产的途径,因为我们预计将有更多涉及IAV VLP疫苗的临床试验,从而在不久的将来实现这些疫苗的商业化。
{"title":"Multifaceted virus-like particles: Navigating towards broadly effective influenza A virus vaccines","authors":"Jaffar Ali Muhamad Norizwan,&nbsp;Wen Siang Tan","doi":"10.1016/j.crmicr.2024.100317","DOIUrl":"10.1016/j.crmicr.2024.100317","url":null,"abstract":"<div><div>The threat of influenza A virus (IAV) remains an annual health concern, as almost 500,000 people die each year due to the seasonal flu. Current flu vaccines are highly dependent on embryonated chicken eggs for production, which is time consuming and costly. These vaccines only confer moderate protections in elderly people, and they lack cross-protectivity; thereby requiring annual reformulation to ensure effectiveness against contemporary circulating strains. To address current limitations, new strategies are being sought, with great emphasis given on exploiting IAV's conserved antigens for vaccine development, and by using different vaccine technologies to enhance immunogenicity and expedite vaccine production. Among these technologies, there are growing pre-clinical and clinical studies involving virus-like particles (VLPs), as they are capable to display multiple conserved IAV antigens and augment their immune responses. In this review, we outline recent findings involving broadly effective IAV antigens and strategies to display these antigens on VLPs. Current production systems for IAV VLP vaccines are comprehensively reviewed. Pain-free methods for administration of IAV VLP vaccines through intranasal and transdermal routes, as well as the mechanisms in stimulating immune responses are discussed in detail. The future perspectives of VLPs in IAV vaccine development are discussed, particularly concerning their potentials in overcoming current immunological limitations of IAV vaccines, and their inherent advantages in exploring intranasal vaccination studies. We also propose avenues to expedite VLP vaccine production, as we envision that there will be more clinical trials involving IAV VLP vaccines, leading to commercialization of these vaccines in the near future.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100317"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preventive effect of flavor/fragrance components on SARS-CoV-2 infections 风味/香味成分对SARS-CoV-2感染的预防作用
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100335
Hidenobu Senpuku , Hiroyuki Kato
The SARS-CoV-2 infection has spread to various areas of the world, and the number of infected people, seriously ill people, and deaths have increased in 2020∼2023. It is important to suppress the spread of virus from infected people to non-infected people in order to prevent the disease from becoming more severe. To protect widespread of virus, flavor/fragrances composition was selected as a convenient effective material to protect the inhibition. It was previously investigated whether flavor/fragrances composition inhibit the binding between the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein and host angiotensin converting enzyme 2 (ACE2) in the infection model assay. This binding lead to natural infection of SARS-CoV-2 to tissues. In a previous report, it was found that some Flavor/fragrances compositions strongly inhibited the binding between RBD and ACE2. To clarify whether these flavor/fragrances compositions actually inhibit the infection of SARS-CoV-2, the inhibition assay of infection to VeroE6/TMPRSS2 cells, the inhibition model in vitro, were performed by the treatment of these compositions. Some flavor/fragrances compositions excepting for cinnamyl alcohol, 0.25 %, strongly inhibited the infection of SARS-CoV-2 to VeroE6/TMPRSS2 cells because cinnamyl alcohol could not be completely melted by PBS (pH 7.4) containing 1.5 % Tween 20 and 0.5 % BSA. Among fragrance compounds, cinnamon flavor and cinnamon mint had stronger inhibition effects on the infection effects on SARS-C0V-2 than others. The strategy of using flavor/fragrances compositions such as cinnamon flavor and cinnamon mint may be useful to protect widespread of SARS-CoV-2 in their daily lives.
SARS-CoV-2感染已扩散到世界各地,2020 ~ 2023年的感染者、重症患者和死亡人数都在增加。重要的是要抑制病毒从感染者向非感染者的传播,以防止疾病变得更加严重。为了保护病毒的传播,选择香精/香料组合物作为方便有效的材料来保护病毒的抑制作用。在感染模型试验中,研究人员先前研究了风味/香料成分是否抑制SARS-CoV-2刺突蛋白受体结合域(RBD)与宿主血管紧张素转换酶2 (ACE2)之间的结合。这种结合导致SARS-CoV-2对组织的自然感染。在先前的报道中,发现一些风味/香味成分强烈抑制RBD与ACE2的结合。为了明确这些香料/香料组合物是否真的抑制了SARS-CoV-2的感染,我们通过这些组合物处理对体外抑制模型VeroE6/TMPRSS2细胞的感染进行了抑制实验。除肉桂醇(0.25%)外,其他风味/香料成分对VeroE6/TMPRSS2细胞的感染有较强的抑制作用,因为肉桂醇不能被含有1.5% Tween 20和0.5% BSA的PBS (pH 7.4)完全融化。香料化合物中,肉桂香精和肉桂薄荷对SARS-C0V-2的感染抑制作用较强。使用肉桂香精和肉桂薄荷等香料/香料组合物的策略可能有助于保护日常生活中SARS-CoV-2的广泛传播。
{"title":"Preventive effect of flavor/fragrance components on SARS-CoV-2 infections","authors":"Hidenobu Senpuku ,&nbsp;Hiroyuki Kato","doi":"10.1016/j.crmicr.2024.100335","DOIUrl":"10.1016/j.crmicr.2024.100335","url":null,"abstract":"<div><div>The SARS-CoV-2 infection has spread to various areas of the world, and the number of infected people, seriously ill people, and deaths have increased in 2020∼2023. It is important to suppress the spread of virus from infected people to non-infected people in order to prevent the disease from becoming more severe. To protect widespread of virus, flavor/fragrances composition was selected as a convenient effective material to protect the inhibition. It was previously investigated whether flavor/fragrances composition inhibit the binding between the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein and host angiotensin converting enzyme 2 (ACE2) in the infection model assay. This binding lead to natural infection of SARS-CoV-2 to tissues. In a previous report, it was found that some Flavor/fragrances compositions strongly inhibited the binding between RBD and ACE2. To clarify whether these flavor/fragrances compositions actually inhibit the infection of SARS-CoV-2, the inhibition assay of infection to VeroE6/TMPRSS2 cells, the inhibition model in vitro, were performed by the treatment of these compositions. Some flavor/fragrances compositions excepting for cinnamyl alcohol, 0.25 %, strongly inhibited the infection of SARS-CoV-2 to VeroE6/TMPRSS2 cells because cinnamyl alcohol could not be completely melted by PBS (pH 7.4) containing 1.5 % Tween 20 and 0.5 % BSA. Among fragrance compounds, cinnamon flavor and cinnamon mint had stronger inhibition effects on the infection effects on SARS-C0V-2 than others. The strategy of using flavor/fragrances compositions such as cinnamon flavor and cinnamon mint may be useful to protect widespread of SARS-CoV-2 in their daily lives.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100335"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of disinfection methods used in the slaughterhouse environment on microbiome diversity throughout the meat production chain
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100336
Wissal Naim , Julia Manetsberger , Leyre Lavilla Lerma , Nabil Benomar , Natacha Caballero Gómez , Inmaculada S. Cuesta-Bertomeu , Jaime Ángel Gata Díaz , Hikmate Abriouel
Slaughterhouse environments are prone to microbial contamination, influenced by factors like set-up, size and area as well as disinfection practices. Thus, effective control measures are crucial to prevent the spread of pathogens and their contaminant genes (antimicrobial resistance genes and virulence factors) throughout the food chain. In the present study, we assessed the microbial contamination in environmental surfaces of three slaughterhouses located in the Jaén province (Spain). We also evaluated the impact of different disinfection strategies on microbial loads and diversity by means of culture dependent and independent methods. The results revealed a statistically significant inter- and intra-specific differences in microbial loads including the most important pathogens such as pseudomonads, staphylococci, Escherichia coli, Salmonella sp. and Campylobacter jejuni. Disinfection strategies using routine disinfectant (used by the slaughterhouse), HLE disinfectant, UV, or combinations thereof showed varying effectiveness. The newly developed sustainable HLE disinfectant was most effective, while UV had the lowest disinfection strength, and routine disinfectants failed to eradicate all pathogens.
Metagenomic analysis identified Pseudomonadota as the dominant phylum, followed by Actinomycetota and Bacteroidota. Results furthermore indicated shifts from sacrifice to cold rooms, with an increase in Gammaproteobacteria, particularly Moraxellaceae (represented by Psychrobacter cryohalolentis) over Acinetobacter sp. In conclusion, this study highlights the potential of HLE disinfectant (alone or in combination with the routine disinfectant) as a more effective disinfection measure on environmental surfaces, particularly for combating multi-drug resistant pathogens compared to other disinfection methods currently used.
{"title":"Impact of disinfection methods used in the slaughterhouse environment on microbiome diversity throughout the meat production chain","authors":"Wissal Naim ,&nbsp;Julia Manetsberger ,&nbsp;Leyre Lavilla Lerma ,&nbsp;Nabil Benomar ,&nbsp;Natacha Caballero Gómez ,&nbsp;Inmaculada S. Cuesta-Bertomeu ,&nbsp;Jaime Ángel Gata Díaz ,&nbsp;Hikmate Abriouel","doi":"10.1016/j.crmicr.2024.100336","DOIUrl":"10.1016/j.crmicr.2024.100336","url":null,"abstract":"<div><div>Slaughterhouse environments are prone to microbial contamination, influenced by factors like set-up, size and area as well as disinfection practices. Thus, effective control measures are crucial to prevent the spread of pathogens and their contaminant genes (antimicrobial resistance genes and virulence factors) throughout the food chain. In the present study, we assessed the microbial contamination in environmental surfaces of three slaughterhouses located in the Jaén province (Spain). We also evaluated the impact of different disinfection strategies on microbial loads and diversity by means of culture dependent and independent methods. The results revealed a statistically significant inter- and intra-specific differences in microbial loads including the most important pathogens such as pseudomonads, staphylococci, <em>Escherichia coli, Salmonella</em> sp. and <em>Campylobacter jejuni</em>. Disinfection strategies using routine disinfectant (used by the slaughterhouse), HLE disinfectant, UV, or combinations thereof showed varying effectiveness. The newly developed sustainable HLE disinfectant was most effective, while UV had the lowest disinfection strength, and routine disinfectants failed to eradicate all pathogens.</div><div>Metagenomic analysis identified Pseudomonadota as the dominant phylum, followed by Actinomycetota and Bacteroidota. Results furthermore indicated shifts from sacrifice to cold rooms, with an increase in Gammaproteobacteria, particularly <em>Moraxellaceae</em> (represented by <em>Psychrobacter cryohalolentis</em>) over <em>Acinetobacter</em> sp. In conclusion, this study highlights the potential of HLE disinfectant (alone or in combination with the routine disinfectant) as a more effective disinfection measure on environmental surfaces, particularly for combating multi-drug resistant pathogens compared to other disinfection methods currently used.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100336"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interrelationship between altered metabolites and the gut microbiota in people living with HIV with different immune responses to antiretroviral therapy
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100340
Xuebin Tian , Zhongyao Gao , Yiwen Xie , Xiangyun Lu , Yulong Zhao , Peng Yao , Mingqing Dong , Lifeng Yu , Nanping Wu

Background

Antiretroviral therapy (ART) effectively reduces opportunistic infections and mortality in people living with HIV (PLWH); however, some patients exhibit poor immune recovery. This study explores the connections among immune responses, metabolites, and the gut microbiota in PLWH with differing reactions to ART.

Methods

We analyzed the gut microbiota composition, metabolites, and immune markers in 38 PLWH who showed an immunological response (IR) and 32 who did not (INR), as classified according to CD4+ T-cell levels after 24 months of ART. Additionally, in vitro assays using cell counting kit 8, flow cytometry, and quantitative real-time reverse transcription PCR were employed to assess the effects of the metabolites on cell viability, immune marker expression, and cytokine levels.

Results

Gut microbiota and metabolic profiles differed significantly between the IR and INR groups. Enterococcus was more abundant in the INR group, whereas [Ruminococcus]_gnavus_group levels were reduced. Significant metabolic pathway alterations included decreased folate biosynthesis and biotin metabolism. We observed negative associations of Parabacteroides with activation markers on CD4+ T-cells, and positive correlations with CD4/CD8 ratios. Enterococcus showed inverse relationships with these markers. Indole-3-acetyl-beta-1-D-glucoside (area under the curve value = 0.8931), had the best discriminatory ability. Further experiments showed that Indole-3-acetyl-beta-1-D-glucoside significantly decreased the proportions of CD4+CD57+, effector CD4+, CD4+PD1+, CD8+CD57+, effector CD8+, and CD8+HLA-DR+ T cells. Moreover, mRNA expression analysis showed that Indole-3-acetyl-beta-1-D-glucoside treatment led to a suppression of pro-inflammatory cytokines.

Conclusion

The multi-omics approach highlighted potential biomarkers for immune recovery in HIV, suggesting avenues for further research into treatment strategies.
{"title":"Interrelationship between altered metabolites and the gut microbiota in people living with HIV with different immune responses to antiretroviral therapy","authors":"Xuebin Tian ,&nbsp;Zhongyao Gao ,&nbsp;Yiwen Xie ,&nbsp;Xiangyun Lu ,&nbsp;Yulong Zhao ,&nbsp;Peng Yao ,&nbsp;Mingqing Dong ,&nbsp;Lifeng Yu ,&nbsp;Nanping Wu","doi":"10.1016/j.crmicr.2025.100340","DOIUrl":"10.1016/j.crmicr.2025.100340","url":null,"abstract":"<div><h3>Background</h3><div>Antiretroviral therapy (ART) effectively reduces opportunistic infections and mortality in people living with HIV (PLWH); however, some patients exhibit poor immune recovery. This study explores the connections among immune responses, metabolites, and the gut microbiota in PLWH with differing reactions to ART.</div></div><div><h3>Methods</h3><div>We analyzed the gut microbiota composition, metabolites, and immune markers in 38 PLWH who showed an immunological response (IR) and 32 who did not (INR), as classified according to CD4+ T-cell levels after 24 months of ART. Additionally, in vitro assays using cell counting kit 8, flow cytometry, and quantitative real-time reverse transcription PCR were employed to assess the effects of the metabolites on cell viability, immune marker expression, and cytokine levels.</div></div><div><h3>Results</h3><div>Gut microbiota and metabolic profiles differed significantly between the IR and INR groups. <em>Enterococcus</em> was more abundant in the INR group, whereas <em>[Ruminococcus]_gnavus_group</em> levels were reduced. Significant metabolic pathway alterations included decreased folate biosynthesis and biotin metabolism. We observed negative associations of <em>Parabacteroides</em> with activation markers on CD4+ T-cells, and positive correlations with CD4/CD8 ratios. <em>Enterococcus</em> showed inverse relationships with these markers. Indole-3-acetyl-beta-1-D-glucoside (area under the curve value = 0.8931), had the best discriminatory ability. Further experiments showed that Indole-3-acetyl-beta-1-D-glucoside significantly decreased the proportions of CD4+CD57+, effector CD4+, CD4+PD1+, CD8+CD57+, effector CD8+, and CD8+HLA-DR+ T cells. Moreover, mRNA expression analysis showed that Indole-3-acetyl-beta-1-D-glucoside treatment led to a suppression of pro-inflammatory cytokines.</div></div><div><h3>Conclusion</h3><div>The multi-omics approach highlighted potential biomarkers for immune recovery in HIV, suggesting avenues for further research into treatment strategies.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100340"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic conversion of phenol to polyhydroxyalkanoate (PHA) for addressing dual environmental challenges: A review
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100352
Izzati Sabri , Mohd Zulkhairi Mohd Yusoff , Nor Azlan Nor Muhammad , Li Sim Ho , Norhayati Ramli
A sustainable approach to microbial polyhydroxyalkanoate (PHA) production involves utilizing waste as a substrate, which can include toxic pollutants like phenol as a carbon feedstock. Phenol-contaminated effluents offer cost-effective and readily available resources for PHA production, while simultaneously addressing phenol contamination issues. Understanding the metabolic conversion of phenol to PHA is crucial to enhance its efficiency, especially considering phenol's toxicity to microbial cells and the substrate-dependent nature of microbial PHA production. In this review, the mechanisms of phenol biodegradation and PHA biosynthesis are first independently elucidated to comprehend the role of bacteria in these processes. Phenol can be metabolized aerobically via various pathways, including catechol meta-cleavage I and II, catechol ortho-cleavage, protocatechuate ortho-cleavage, and protocatechuate meta-cleavage, as well as anaerobically via 4-hydrozybenzoate and/or n-caproate formation. Meanwhile, PHA can be synthesized through the acetoacetyl-CoA (pathway I), de novo fatty acids synthesis (pathway II), β-oxidation (pathway III), and the tricarboxylic acid (TCA) cycle, with the induction of these pathways are highly dependent on the substrate. Given that the link between these two mechanisms was not comprehensively reported before, the second part of the review delve into understanding phenol conversion into PHA, specifically polyhydroxybutyrate (PHB). While phenol toxicity can inhibit bacterial performance, it can be alleviated through the utilization of microbial mixed culture (MMC), which offers a wider range of metabolic capabilities. Utilizing phenol as a carbon feedstock for PHB accumulation could offer a viable approach to boost PHA's commercialization while addressing the issue of phenol pollution.
{"title":"Metabolic conversion of phenol to polyhydroxyalkanoate (PHA) for addressing dual environmental challenges: A review","authors":"Izzati Sabri ,&nbsp;Mohd Zulkhairi Mohd Yusoff ,&nbsp;Nor Azlan Nor Muhammad ,&nbsp;Li Sim Ho ,&nbsp;Norhayati Ramli","doi":"10.1016/j.crmicr.2025.100352","DOIUrl":"10.1016/j.crmicr.2025.100352","url":null,"abstract":"<div><div>A sustainable approach to microbial polyhydroxyalkanoate (PHA) production involves utilizing waste as a substrate, which can include toxic pollutants like phenol as a carbon feedstock. Phenol-contaminated effluents offer cost-effective and readily available resources for PHA production, while simultaneously addressing phenol contamination issues. Understanding the metabolic conversion of phenol to PHA is crucial to enhance its efficiency, especially considering phenol's toxicity to microbial cells and the substrate-dependent nature of microbial PHA production. In this review, the mechanisms of phenol biodegradation and PHA biosynthesis are first independently elucidated to comprehend the role of bacteria in these processes. Phenol can be metabolized aerobically via various pathways, including catechol <em>meta</em>-cleavage I and II, catechol <em>ortho</em>-cleavage, protocatechuate <em>ortho</em>-cleavage, and protocatechuate <em>meta</em>-cleavage, as well as anaerobically via 4-hydrozybenzoate and/or n-caproate formation. Meanwhile, PHA can be synthesized through the acetoacetyl-CoA (pathway I), <em>de novo</em> fatty acids synthesis (pathway II), β-oxidation (pathway III), and the tricarboxylic acid (TCA) cycle, with the induction of these pathways are highly dependent on the substrate. Given that the link between these two mechanisms was not comprehensively reported before, the second part of the review delve into understanding phenol conversion into PHA, specifically polyhydroxybutyrate (PHB). While phenol toxicity can inhibit bacterial performance, it can be alleviated through the utilization of microbial mixed culture (MMC), which offers a wider range of metabolic capabilities. Utilizing phenol as a carbon feedstock for PHB accumulation could offer a viable approach to boost PHA's commercialization while addressing the issue of phenol pollution.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100352"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100353
Kateřina Snopková , Karel Sedlář , Dana Nováková , Eva Staňková , Ivo Sedláček , Ondřej Šedo , Veronika Holá
The extreme conditions of the Antarctic environment have driven the evolution of highly specialized microbial communities with unique adaptations. In this study, we characterized five Pseudomonas isolates from James Ross Island, which displayed notable taxonomic and metabolite features. Phylogenomic analysis revealed that strain P2663T occupies a distinct phylogenetic position within the Pseudomonas genus, related to species Pseudomonas svalbardensis, Pseudomonas silesiensis, Pseudomonas mucoides, Pseudomonas prosekii, and Pseudomonas gregormendelii. The novelty of five Antarctic isolates was further confirmed through analyses of housekeeping genes, ribotyping, and REP-PCR profiling. MALDI-TOF MS analysis identified 11 unique mass spectrometry signals shared by the Antarctic isolates, which were not detected in other related species. Additionally, chemotaxonomic characterization, including fatty acid composition, demonstrated similarities with related Pseudomonas species. Phenotypic assessments revealed distinctive biochemical and physiological traits.
In-depth genomic analysis of strain P2663T uncovered numerous genes which could be involved in survival in extreme Antarctic conditions, including those encoding cold-shock and heat-shock proteins, oxidative and osmotic stress response proteins, and carotenoid-like pigments. Genome mining further revealed several biosynthetic gene clusters, some of which are associated with antimicrobial activity. Functional assays supported the antimicrobial capabilities of this novel species, showing antagonistic effects against clinical isolates of Pseudomonas aeruginosa, possibly mediated by tailocins (phage tail-like particles).
This comprehensive polyphasic study characterized a new cold-adapted species, for which we propose the name Pseudomonas rossensis sp. nov.
{"title":"Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa","authors":"Kateřina Snopková ,&nbsp;Karel Sedlář ,&nbsp;Dana Nováková ,&nbsp;Eva Staňková ,&nbsp;Ivo Sedláček ,&nbsp;Ondřej Šedo ,&nbsp;Veronika Holá","doi":"10.1016/j.crmicr.2025.100353","DOIUrl":"10.1016/j.crmicr.2025.100353","url":null,"abstract":"<div><div>The extreme conditions of the Antarctic environment have driven the evolution of highly specialized microbial communities with unique adaptations. In this study, we characterized five <em>Pseudomonas</em> isolates from James Ross Island, which displayed notable taxonomic and metabolite features. Phylogenomic analysis revealed that strain P2663<sup>T</sup> occupies a distinct phylogenetic position within the <em>Pseudomonas</em> genus, related to species <em>Pseudomonas svalbardensis, Pseudomonas silesiensis, Pseudomonas mucoides, Pseudomonas prosekii</em>, and <em>Pseudomonas gregormendelii</em>. The novelty of five Antarctic isolates was further confirmed through analyses of housekeeping genes, ribotyping, and REP-PCR profiling. MALDI-TOF MS analysis identified 11 unique mass spectrometry signals shared by the Antarctic isolates, which were not detected in other related species. Additionally, chemotaxonomic characterization, including fatty acid composition, demonstrated similarities with related <em>Pseudomonas</em> species. Phenotypic assessments revealed distinctive biochemical and physiological traits.</div><div>In-depth genomic analysis of strain P2663<sup>T</sup> uncovered numerous genes which could be involved in survival in extreme Antarctic conditions, including those encoding cold-shock and heat-shock proteins, oxidative and osmotic stress response proteins, and carotenoid-like pigments. Genome mining further revealed several biosynthetic gene clusters, some of which are associated with antimicrobial activity. Functional assays supported the antimicrobial capabilities of this novel species, showing antagonistic effects against clinical isolates of <em>Pseudomonas aeruginosa</em>, possibly mediated by tailocins (phage tail-like particles).</div><div>This comprehensive polyphasic study characterized a new cold-adapted species, for which we propose the name <em>Pseudomonas rossensis</em> sp. nov.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100353"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the role of the PhoP master regulator in arsenite resistance through ackA downregulation in Lacticaseibacillus paracasei 通过下调副酸乳杆菌中的 ackA,揭示 PhoP 主调节因子在亚砷酸盐抗性中的作用
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100357
Daniela Corrales , Cristina Alcántara , Dinoraz Vélez , Vicenta Devesa , Vicente Monedero , Manuel Zúñiga
In bacteria, the two-component system PhoPR plays an important role in regulating many genes related to phosphate uptake and metabolism. In Lacticaseibacillus paracasei inactivation of the response regulator PhoP results in increased resistance to arsenite [As(III)]. A comparative transcriptomic analysis revealed that the absence of PhoP has a strong effect on the transcriptome, with about 57.5 % of Lc. paracasei genes being differentially expressed, although only 92 of the upregulated genes and 23 of the downregulated genes reached a fold change greater than 2. Among them, the phnDCEB cluster, encoding a putative ABC phosphonate transporter and the acetate kinase encoding gene ackA (LCABL_01600) were downregulated tenfold and sevenfold, respectively. In vitro binding assays with selected PhoP-regulated genes showed that phosphorylation of PhoP stimulated its binding to the promoter regions of pstS (phosphate ABC transporter binding subunit), phnD and glnA glutamine synthetase) whereas no binding to the poxL (pyruvate oxidase) or ackA putative promoter regions was detected. This result identified for the first time three genes/operons belonging to the Pho regulon in a Lactobacillaceae species. Mapping of the reads obtained in the transcriptomic analysis revealed that transcription of ackA was severely diminished in the PhoP mutant after a hairpin structure located within the ackA coding region. Inactivation of phnD did not affect As(III) resistance whereas inactivation of ackA resulted in the same level of resistance as that observed in the PhoP mutant. These finding strongly suggests that PhoP mutant As(III) resistance is due to downregulation of ackA. Possible mechanisms of action are discussed.
{"title":"Unveiling the role of the PhoP master regulator in arsenite resistance through ackA downregulation in Lacticaseibacillus paracasei","authors":"Daniela Corrales ,&nbsp;Cristina Alcántara ,&nbsp;Dinoraz Vélez ,&nbsp;Vicenta Devesa ,&nbsp;Vicente Monedero ,&nbsp;Manuel Zúñiga","doi":"10.1016/j.crmicr.2025.100357","DOIUrl":"10.1016/j.crmicr.2025.100357","url":null,"abstract":"<div><div>In bacteria, the two-component system PhoPR plays an important role in regulating many genes related to phosphate uptake and metabolism. In <em>Lacticaseibacillus paracasei</em> inactivation of the response regulator PhoP results in increased resistance to arsenite [As(III)]. A comparative transcriptomic analysis revealed that the absence of PhoP has a strong effect on the transcriptome, with about 57.5 % of <em>Lc. paracasei</em> genes being differentially expressed, although only 92 of the upregulated genes and 23 of the downregulated genes reached a fold change greater than 2. Among them, the <em>phnDCEB</em> cluster, encoding a putative ABC phosphonate transporter and the acetate kinase encoding gene <em>ackA</em> (LCABL_01600) were downregulated tenfold and sevenfold, respectively. In vitro binding assays with selected PhoP-regulated genes showed that phosphorylation of PhoP stimulated its binding to the promoter regions of <em>pstS</em> (phosphate ABC transporter binding subunit), <em>phnD</em> and <em>glnA</em> glutamine synthetase) whereas no binding to the <em>poxL</em> (pyruvate oxidase) or <em>ackA</em> putative promoter regions was detected. This result identified for the first time three genes/operons belonging to the Pho regulon in a <em>Lactobacillaceae</em> species. Mapping of the reads obtained in the transcriptomic analysis revealed that transcription of <em>ackA</em> was severely diminished in the PhoP mutant after a hairpin structure located within the <em>ackA</em> coding region. Inactivation of <em>phnD</em> did not affect As(III) resistance whereas inactivation of <em>ackA</em> resulted in the same level of resistance as that observed in the PhoP mutant. These finding strongly suggests that PhoP mutant As(III) resistance is due to downregulation of <em>ackA</em>. Possible mechanisms of action are discussed.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100357"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seroprevalence of melioidosis and its associated risk factors –A population-based study in Odisha, Eastern India
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100360
Bijayini Behera , Arvind K Singh , Mohammad Ahmad , Lipipuspa Rout , Jayanti Jena , Asmita Patnaik , Pradeep Behera , Payal Priyadarshini , Dhruv Pandey , Po-Lin Chan , Biswa Prakash Dutta , Prasanta R. Mohapatra , Jitendriya Amrit Pritam , Srujana Mohanty , Ashoka Mahapatra , Abhisek Mishra
Melioidosis, caused by Burkholderia pseudomallei, is an emerging disease in Odisha, a state in eastern coastal India. Difficult to diagnose, the disease is likely to be severely underreported. Seroprevalence studies in the general population are deemed necessary for an estimate of melioidosis endemicity and to explore the associated risk factors. A population-based cross-sectional seroprevalence study was conducted using Indirect Hemagglutination Assay (IHA) among 1920 participants aged 5–60 years residing in six out of thirty districts of Odisha from August to December 2023. Seropositivity was defined as an IHA titer ≥ 1:20. The risk factors associated with seropositivity were determined. Out of 1920 individuals, 1215 (63.3 %) were females and 1680 (87.5 %) were rural residents. 410 out of 1920 individuals had IHA titer ≥ 1:20, contributing to an overall prevalence of 20.9 % [95 % CI: 19.0 % - 22.7 %]. Seropositivity was highest among those aged 21 to 30 years [23.2 % (95 % CI: 19.2 % - 27.4 %)], and in females [21.7 % (95 % CI: 19.4 % – 24.1 %)]. The odds of seropositivity were 1.64 [95 % CI: 1.097 - 2.436, p= 0.016] times higher among rural residents than urban residents with people engaged in cultivation, daily activities involving soil, and water bodies having higher odds of seropositivity (Crude Odds Ratios, 1.22, 1.11 and 1.17 respectively).The present study, covering a single post-monsoon season in Odisha, has yielded a 20.9 % melioidosis seropositivity, matching previous Indian studies with B. pseudomallei seropositivity rates ranging from 20–29 %. The study indicates towards widespread environmental presence of B. pseudomallei, more so in rural areas and thus risk of clinical melioidosis. Adoption of public health interventions as well creation of awareness is of paramount importance in such a scenario.
{"title":"Seroprevalence of melioidosis and its associated risk factors –A population-based study in Odisha, Eastern India","authors":"Bijayini Behera ,&nbsp;Arvind K Singh ,&nbsp;Mohammad Ahmad ,&nbsp;Lipipuspa Rout ,&nbsp;Jayanti Jena ,&nbsp;Asmita Patnaik ,&nbsp;Pradeep Behera ,&nbsp;Payal Priyadarshini ,&nbsp;Dhruv Pandey ,&nbsp;Po-Lin Chan ,&nbsp;Biswa Prakash Dutta ,&nbsp;Prasanta R. Mohapatra ,&nbsp;Jitendriya Amrit Pritam ,&nbsp;Srujana Mohanty ,&nbsp;Ashoka Mahapatra ,&nbsp;Abhisek Mishra","doi":"10.1016/j.crmicr.2025.100360","DOIUrl":"10.1016/j.crmicr.2025.100360","url":null,"abstract":"<div><div>Melioidosis, caused by <em>Burkholderia pseudomallei</em>, is an emerging disease in Odisha, a state in eastern coastal India. Difficult to diagnose, the disease is likely to be severely underreported. Seroprevalence studies in the general population are deemed necessary for an estimate of melioidosis endemicity and to explore the associated risk factors. A population-based cross-sectional seroprevalence study was conducted using Indirect Hemagglutination Assay (IHA) among 1920 participants aged 5–60 years residing in six out of thirty districts of Odisha from August to December 2023. Seropositivity was defined as an IHA titer ≥ 1:20. The risk factors associated with seropositivity were determined. Out of 1920 individuals, 1215 (63.3 %) were females and 1680 (87.5 %) were rural residents. 410 out of 1920 individuals had IHA titer ≥ 1:20, contributing to an overall prevalence of 20.9 % [95 % CI: 19.0 % - 22.7 %]. Seropositivity was highest among those aged 21 to 30 years [23.2 % (95 % CI: 19.2 % - 27.4 %)], and in females [21.7 % (95 % CI: 19.4 % – 24.1 %)]. The odds of seropositivity were 1.64 [95 % CI: 1.097 - 2.436, p= 0.016] times higher among rural residents than urban residents with people engaged in cultivation, daily activities involving soil, and water bodies having higher odds of seropositivity (Crude Odds Ratios, 1.22, 1.11 and 1.17 respectively).The present study, covering a single post-monsoon season in Odisha, has yielded a 20.9 % melioidosis seropositivity, matching previous Indian studies with <em>B. pseudomallei</em> seropositivity rates ranging from 20–29 %. The study indicates towards widespread environmental presence of <em>B. pseudomallei</em>, more so in rural areas and thus risk of clinical melioidosis. Adoption of public health interventions as well creation of awareness is of paramount importance in such a scenario.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100360"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human microbiome in post-acute COVID-19 syndrome (PACS) 急性后COVID-19综合征(PACS)中的人类微生物组
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100324
Arezoo Fallah , Hamid Sedighian , Reza Kachuei , Abbas Ali Imani Fooladi
The global COVID-19 pandemic, which began in 2019, is still ongoing. SARS-CoV-2, also known as the severe acute respiratory syndrome coronavirus 2, is the causative agent. Diarrhea, nausea, and vomiting are common GI symptoms observed in a significant number of COVID-19 patients. Additionally, the respiratory and GI tracts express high level of transmembrane protease serine 2 (TMPRSS2) and angiotensin-converting enzyme-2 (ACE2), making them primary sites for human microbiota and targets for SARS-CoV-2 infection. A growing body of research indicates that individuals with COVID-19 and post-acute COVID-19 syndrome (PACS) exhibit considerable alterations in their microbiome. In various human disorders, including diabetes, obesity, cancer, ulcerative colitis, Crohn's disease, and several viral infections, the microbiota play a significant immunomodulatory role.
In this review, we investigate the potential therapeutic implications of the interactions between host microbiota and COVID-19. Microbiota-derived metabolites and components serve as primary mediators of microbiota-host interactions, influencing host immunity. We discuss the various mechanisms through which these metabolites or components produced by the microbiota impact the host's immune response to SARS-CoV-2 infection. Additionally, we address confounding factors in microbiome studies. Finally, we examine and discuss about a range of potential microbiota-based prophylactic measures and treatments for COVID-19 and PACS, as well as their effects on clinical outcomes and disease severity.
2019年开始的全球COVID-19大流行仍在持续。SARS-CoV-2,也被称为严重急性呼吸综合征冠状病毒2,是病原体。腹泻、恶心和呕吐是大量COVID-19患者常见的胃肠道症状。此外,呼吸道和胃肠道高水平表达跨膜蛋白酶丝氨酸2 (TMPRSS2)和血管紧张素转换酶-2 (ACE2),使其成为人类微生物群的主要位点和SARS-CoV-2感染的靶点。越来越多的研究表明,COVID-19和急性后COVID-19综合征(PACS)患者的微生物组发生了相当大的变化。在各种人类疾病中,包括糖尿病、肥胖、癌症、溃疡性结肠炎、克罗恩病和几种病毒感染,微生物群起着重要的免疫调节作用。在这篇综述中,我们研究了宿主微生物群与COVID-19相互作用的潜在治疗意义。微生物衍生的代谢物和成分是微生物与宿主相互作用的主要介质,影响宿主免疫。我们讨论了微生物群产生的代谢物或成分影响宿主对SARS-CoV-2感染的免疫反应的各种机制。此外,我们解决了微生物组研究中的混杂因素。最后,我们研究和讨论了一系列潜在的基于微生物群的COVID-19和PACS预防措施和治疗方法,以及它们对临床结果和疾病严重程度的影响。
{"title":"Human microbiome in post-acute COVID-19 syndrome (PACS)","authors":"Arezoo Fallah ,&nbsp;Hamid Sedighian ,&nbsp;Reza Kachuei ,&nbsp;Abbas Ali Imani Fooladi","doi":"10.1016/j.crmicr.2024.100324","DOIUrl":"10.1016/j.crmicr.2024.100324","url":null,"abstract":"<div><div>The global COVID-19 pandemic, which began in 2019, is still ongoing. SARS-CoV-2, also known as the severe acute respiratory syndrome coronavirus 2, is the causative agent. Diarrhea, nausea, and vomiting are common GI symptoms observed in a significant number of COVID-19 patients. Additionally, the respiratory and GI tracts express high level of transmembrane protease serine 2 (TMPRSS2) and angiotensin-converting enzyme-2 (ACE2), making them primary sites for human microbiota and targets for SARS-CoV-2 infection. A growing body of research indicates that individuals with COVID-19 and post-acute COVID-19 syndrome (PACS) exhibit considerable alterations in their microbiome. In various human disorders, including diabetes, obesity, cancer, ulcerative colitis, Crohn's disease, and several viral infections, the microbiota play a significant immunomodulatory role.</div><div>In this review, we investigate the potential therapeutic implications of the interactions between host microbiota and COVID-19. Microbiota-derived metabolites and components serve as primary mediators of microbiota-host interactions, influencing host immunity. We discuss the various mechanisms through which these metabolites or components produced by the microbiota impact the host's immune response to SARS-CoV-2 infection. Additionally, we address confounding factors in microbiome studies. Finally, we examine and discuss about a range of potential microbiota-based prophylactic measures and treatments for COVID-19 and PACS, as well as their effects on clinical outcomes and disease severity.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100324"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Current Research in Microbial Sciences
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1