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“Reflexions on the role, diversity, conservation and management of the genetic microbial resources in Agriculture”
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100365
María del Pilar Rodríguez Guzmán , Ismael Fernando Chávez Díaz , Lily Xochilt Zelaya Molina
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引用次数: 0
Unearthing the burden of melioidosis in North India – an emerging threat in a non-endemic region
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100344
Shweta Raina , Disha Gautam , Rohit Kumar , Kavita Sisodia , Chiranjay Mukhopadhyay , Harpreet Kaur , Mohammed Ashiq , Rushika Saksena
Burkholderia pseudomallei (B. pseudomallei) is the causative agent of the high-mortality disease called melioidosis. It is a severe infection that can be misdiagnosed due to variable presentation and low awareness among clinicians of the disease. It is endemic in India and well-described in southern and eastern coastal states. In the last decade, sporadic cases of melioidosis have been diagnosed in North Indian states, predominantly Rajasthan and Gujarat. The reported cases highlight the many risk factors for infection in this region that was not previously recognised as being endemic for melioidosis, including high prevalence of diabetes mellitus, alcoholism, and large rural population engaged in paddy cultivation. Climate change results in frequent flooding and waterlogging in urban areas, leading to exposure of soil harbouring B. pseudomallei, thus a contributing factor to the rise in cases in cities. As North India has not previously been considered an endemic region for melioidosis, wider awareness amongst clinicians and laboratorians is essential for early identification of symptoms, testing for B. pseudomallei in microbiology laboratories, and timely management of the disease to save lives lost to misdiagnosis. The present article describes various aspects of melioidosis in North India including diverse clinical manifestations, risk factors, and possible reasons for misdiagnosis and underreporting.
{"title":"Unearthing the burden of melioidosis in North India – an emerging threat in a non-endemic region","authors":"Shweta Raina ,&nbsp;Disha Gautam ,&nbsp;Rohit Kumar ,&nbsp;Kavita Sisodia ,&nbsp;Chiranjay Mukhopadhyay ,&nbsp;Harpreet Kaur ,&nbsp;Mohammed Ashiq ,&nbsp;Rushika Saksena","doi":"10.1016/j.crmicr.2025.100344","DOIUrl":"10.1016/j.crmicr.2025.100344","url":null,"abstract":"<div><div><em>Burkholderia pseudomallei (B. pseudomallei)</em> is the causative agent of the high-mortality disease called melioidosis. It is a severe infection that can be misdiagnosed due to variable presentation and low awareness among clinicians of the disease. It is endemic in India and well-described in southern and eastern coastal states. In the last decade, sporadic cases of melioidosis have been diagnosed in North Indian states, predominantly Rajasthan and Gujarat. The reported cases highlight the many risk factors for infection in this region that was not previously recognised as being endemic for melioidosis, including high prevalence of diabetes mellitus, alcoholism, and large rural population engaged in paddy cultivation. Climate change results in frequent flooding and waterlogging in urban areas, leading to exposure of soil harbouring <em>B. pseudomallei,</em> thus a contributing factor to the rise in cases in cities. As North India has not previously been considered an endemic region for melioidosis, wider awareness amongst clinicians and laboratorians is essential for early identification of symptoms, testing for <em>B. pseudomallei</em> in microbiology laboratories, and timely management of the disease to save lives lost to misdiagnosis. The present article describes various aspects of melioidosis in North India including diverse clinical manifestations, risk factors, and possible reasons for misdiagnosis and underreporting.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100344"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chronic wounds and adaptive Pseudomonas aeruginosa: A phenotypic and genotypic characterization
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100348
Kasandra Buchholtz , Rie Jønsson , Rasmus L. Marvig , Biljana Mojsoska , Karen Angeliki Krogfelt
Phenotypic and genetic diversity is found in varying prevalence in clinical populations where beneficial adaptations enable the bacteria to avoid recognition and eradication by the host immune system. This study aimed to investigate the presence of Pseudomonas aeruginosa in chronic venous leg ulcers wounds over an 8-week time course. This was performed using genomic and phenotypic approaches to understand the survival and persistence of Pseudomonas strains. The findings of this study show that the two patients were colonized with a recurring P. aeruginosa genotype with only minor phenotypic differences and few SNP differences, suggesting that the Pseudomonas isolates present in the wound can survive and proliferate in the host's hostile environment. The results provided from this study will allow us to understand P. aeruginosa colonization during a 8 week time period.
{"title":"Chronic wounds and adaptive Pseudomonas aeruginosa: A phenotypic and genotypic characterization","authors":"Kasandra Buchholtz ,&nbsp;Rie Jønsson ,&nbsp;Rasmus L. Marvig ,&nbsp;Biljana Mojsoska ,&nbsp;Karen Angeliki Krogfelt","doi":"10.1016/j.crmicr.2025.100348","DOIUrl":"10.1016/j.crmicr.2025.100348","url":null,"abstract":"<div><div>Phenotypic and genetic diversity is found in varying prevalence in clinical populations where beneficial adaptations enable the bacteria to avoid recognition and eradication by the host immune system. This study aimed to investigate the presence of <em>Pseudomonas aeruginosa</em> in chronic venous leg ulcers wounds over an 8-week time course. This was performed using genomic and phenotypic approaches to understand the survival and persistence of <em>Pseudomonas</em> strains. The findings of this study show that the two patients were colonized with a recurring <em>P. aeruginosa</em> genotype with only minor phenotypic differences and few SNP differences, suggesting that the <em>Pseudomonas</em> isolates present in the wound can survive and proliferate in the host's hostile environment. The results provided from this study will allow us to understand <em>P. aeruginosa</em> colonization during a 8 week time period.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100348"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heptad repeat 1-derived N peptide inhibitors improve broad-spectrum anti-HIV-1 activity
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100364
Chen Yuan , Jia-Ye Wang , Bu-Yi Wang , Yi-Lin Zhao , Yan Li , Di Li , Hong Ling , Min Zhuang

Background

HIV-1 N-peptide inhibitor (NPI) derived from N-terminal heptad-repeat region (HR1) of gp41 can target C-terminal heptad-repeat region (HR2) or the HR1 to interfere with the formation of endogenous six-helix bundle (6HB). However, the NPI is less active than the C-peptide inhibitor. In this study, we reported three HR1-derived NPIs designed by adding fusion peptide proximal region (FPPR) of gp41 or a trimeric motif MTQ into the N36 peptide and then evaluated their anti-HIV-1 activities.

Methods

Molecular modeling was performed using Swiss Model. The inhibitory activity of NPIs on HIV-1 was assessed by Env-pseudovirus infection assays and cell-cell fusion assays. Interaction between NPIs and HR2 peptides was evaluated by circular dichroism and Native PAGE.

Results

The three newly designed NPIs, FPPR-N36, MTQ-N36, and MTQ-FPPR-N36, exhibited higher anti-HIV-1 activity than N36. The stability of the coiled-coil core formed by three designed NPIs or the 6HB formed by C34 and these NPIs were significantly higher than those of corresponding monomer N36 or isoleucine zipper-engineered trimeric N36 (IZN36). The 50 % inhibitory concentrations (IC50) of MTQ-N36 against HIV-1 infection were at a nanomolar level, lower than those of other tested NPIs. The FPPR-N36 could also inhibit infection of HIV-1 strains that were resistant to N36 and IZN36.

Conclusions

The three newly designed NPIs had inhibitory activity against HIV-1 infection. Among them, MTQ-N36 exhibited a higher potential to inhibit HIV-1 entry than other peptides, and FPPR-N36 might be a promising candidate NPI for suppressing HIV-1 strains that are resistant to conventional NPIs.
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引用次数: 0
Expansion of maltose/sucrose related transporters in Ascomycetes and their association with corresponding disaccharide utilization
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100368
Li Xu , Alessia Manassero , Berend Snel , Ronald P. de Vries , Mao Peng
Sugar transporters (STs) play a crucial role in mediating sugar uptake in fungi and have been increasingly studied due to their important biological roles and industrial potential. In this study, we performed a comprehensive phylogenetic analysis of STs across the fungal kingdom, including species from Ascomycota, Basidiomycota, Mucoromycota and Zoopagomycota. The results revealed a striking diversity of STs among these fungal phyla with respect to their genomic content and predicted sugar specificity. Particularly, we identified a remarkable expansion of maltose/sucrose STs and a strong co-expansion of intracellular α-1,4-glucosidases and invertases in Ascomycota compared to other fungal phyla. In addition, growth profiles support that the utilization of maltose and sucrose across a diverse set of fungi is roughly determined by the presence of both corresponding STs and functionally related hydrolases. This study enhances our understanding of evolutional diversity of fungal STs and provides new insights into metabolic engineering of fungi towards more efficient conversion of plant-derived sugars for relevant industrial applications.
{"title":"Expansion of maltose/sucrose related transporters in Ascomycetes and their association with corresponding disaccharide utilization","authors":"Li Xu ,&nbsp;Alessia Manassero ,&nbsp;Berend Snel ,&nbsp;Ronald P. de Vries ,&nbsp;Mao Peng","doi":"10.1016/j.crmicr.2025.100368","DOIUrl":"10.1016/j.crmicr.2025.100368","url":null,"abstract":"<div><div>Sugar transporters (STs) play a crucial role in mediating sugar uptake in fungi and have been increasingly studied due to their important biological roles and industrial potential. In this study, we performed a comprehensive phylogenetic analysis of STs across the fungal kingdom, including species from Ascomycota, Basidiomycota, Mucoromycota and Zoopagomycota. The results revealed a striking diversity of STs among these fungal phyla with respect to their genomic content and predicted sugar specificity. Particularly, we identified a remarkable expansion of maltose/sucrose STs and a strong co-expansion of intracellular α-1,4-glucosidases and invertases in Ascomycota compared to other fungal phyla. In addition, growth profiles support that the utilization of maltose and sucrose across a diverse set of fungi is roughly determined by the presence of both corresponding STs and functionally related hydrolases. This study enhances our understanding of evolutional diversity of fungal STs and provides new insights into metabolic engineering of fungi towards more efficient conversion of plant-derived sugars for relevant industrial applications.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100368"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of the efficacy of an antimicrobial peptide in the context of cystic fibrosis airways
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100367
Albane Jouault , Inès Jeguirim , Inès Ben Hadj Kaddour , Lhousseine Touqui
Antimicrobial peptides (AMPs) offer a promising alternative to control airway infections with multi-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), which commonly infects patients with cystic fibrosis (CF). However, the behavior of AMPs in the CF context has yet to be fully elucidated. CF airways produce large amounts of proteases and viscous mucus (sputum), which may affect the efficacy of AMPs. The present work aimed to determine whether CF conditions affect the bactericidal efficacy of CAMA, a promising AMP known to kill clinical MRSA strains efficiently. Using a killing assay, we quantified CAMA bactericidal activity on a CF clinical MRSA strain in the presence of several compounds of CF airways, including sputum and bronchial epithelial cells (BECs). Our results indicate that CF sputum impairs the bactericidal efficacy of CAMA. Similar results were observed when CAMA was incubated with an artificial sputum medium (ASM). When used separately, sputum components (DNA, lipids, and mucins) reproduced the inhibitory effects of ASM. Additionally, the bactericidal efficacy of CAMA was also slightly altered when planktonic S. aureus strains were co-cultured with CF BECs. However, CAMA was not active against S. aureus cultured on BEC in biofilm mode, characteristic of chronic infections in CF patients. These findings suggest that although CAMA represents a promising tool to treat MRSA strains, the CF environment may impair the efficacy of this AMP. Identifying strategies to protect AMPs from the deleterious effects of CF sputum is a key priority.
{"title":"Assessment of the efficacy of an antimicrobial peptide in the context of cystic fibrosis airways","authors":"Albane Jouault ,&nbsp;Inès Jeguirim ,&nbsp;Inès Ben Hadj Kaddour ,&nbsp;Lhousseine Touqui","doi":"10.1016/j.crmicr.2025.100367","DOIUrl":"10.1016/j.crmicr.2025.100367","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) offer a promising alternative to control airway infections with multi-resistant bacteria, such as methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), which commonly infects patients with cystic fibrosis (CF). However, the behavior of AMPs in the CF context has yet to be fully elucidated. CF airways produce large amounts of proteases and viscous mucus (sputum), which may affect the efficacy of AMPs. The present work aimed to determine whether CF conditions affect the bactericidal efficacy of CAMA, a promising AMP known to kill clinical MRSA strains efficiently. Using a killing assay, we quantified CAMA bactericidal activity on a CF clinical MRSA strain in the presence of several compounds of CF airways, including sputum and bronchial epithelial cells (BECs). Our results indicate that CF sputum impairs the bactericidal efficacy of CAMA. Similar results were observed when CAMA was incubated with an artificial sputum medium (ASM). When used separately, sputum components (DNA, lipids, and mucins) reproduced the inhibitory effects of ASM. Additionally, the bactericidal efficacy of CAMA was also slightly altered when planktonic <em>S. aureus</em> strains were co-cultured with CF BECs. However, CAMA was not active against <em>S. aureus</em> cultured on BEC in biofilm mode, characteristic of chronic infections in CF patients. These findings suggest that although CAMA represents a promising tool to treat MRSA strains, the CF environment may impair the efficacy of this AMP. Identifying strategies to protect AMPs from the deleterious effects of CF sputum is a key priority.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100367"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant-microbe interactions: PGPM as microbial inoculants/biofertilizers for sustaining crop productivity and soil fertility 植物与微生物的相互作用:PGPM作为维持作物生产力和土壤肥力的微生物接种剂/生物肥料。
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100333
Bibek Laishram , Okram Ricky Devi , Rinjumoni Dutta , T. Senthilkumar , Girish Goyal , Dinesh Kumar Paliwal , Narinder Panotra , Akhtar Rasool
Plant-microbe interactions play pivotal roles in sustaining crop productivity and soil fertility, offering promising avenues for sustainable agricultural practices. This review paper explores the multifaceted interactions between plants and various microorganisms, highlighting their significance in enhancing crop productivity, combating pathogens, and promoting soil health. Understanding these interactions is crucial for harnessing their potential in agricultural systems to address challenges such as food security and environmental sustainability. Therefore, the introduction of beneficial microbes into agricultural ecosystems by bio-augmentation reduces the negative effects of intensive, non-sustainable agriculture on the environment, society, and economy, into the mechanisms underlying the application of plant growth promoting microbes as microbial inoculants/biofertilizers; their interactions, the factors influencing their dynamics, and the implications for agricultural practices, emerging technologies and strategies that leverage plant-microbe interactions for improving crop yields, soil fertility, and overall agricultural sustainability.
植物-微生物相互作用在维持作物生产力和土壤肥力方面发挥着关键作用,为可持续农业实践提供了有希望的途径。本文综述了植物与各种微生物之间多方面的相互作用,强调了它们在提高作物生产力、对抗病原体和促进土壤健康方面的重要意义。了解这些相互作用对于利用它们在农业系统中的潜力来应对粮食安全和环境可持续性等挑战至关重要。因此,通过生物强化将有益微生物引入农业生态系统,可以减少集约化、非可持续农业对环境、社会和经济的负面影响,这是植物生长促进微生物作为微生物接种剂/生物肥料应用的潜在机制;它们的相互作用,影响其动态的因素,以及对农业实践的影响,利用植物-微生物相互作用提高作物产量,土壤肥力和整体农业可持续性的新兴技术和战略。
{"title":"Plant-microbe interactions: PGPM as microbial inoculants/biofertilizers for sustaining crop productivity and soil fertility","authors":"Bibek Laishram ,&nbsp;Okram Ricky Devi ,&nbsp;Rinjumoni Dutta ,&nbsp;T. Senthilkumar ,&nbsp;Girish Goyal ,&nbsp;Dinesh Kumar Paliwal ,&nbsp;Narinder Panotra ,&nbsp;Akhtar Rasool","doi":"10.1016/j.crmicr.2024.100333","DOIUrl":"10.1016/j.crmicr.2024.100333","url":null,"abstract":"<div><div>Plant-microbe interactions play pivotal roles in sustaining crop productivity and soil fertility, offering promising avenues for sustainable agricultural practices. This review paper explores the multifaceted interactions between plants and various microorganisms, highlighting their significance in enhancing crop productivity, combating pathogens, and promoting soil health. Understanding these interactions is crucial for harnessing their potential in agricultural systems to address challenges such as food security and environmental sustainability. Therefore, the introduction of beneficial microbes into agricultural ecosystems by bio-augmentation reduces the negative effects of intensive, non-sustainable agriculture on the environment, society, and economy, into the mechanisms underlying the application of plant growth promoting microbes as microbial inoculants/biofertilizers; their interactions, the factors influencing their dynamics, and the implications for agricultural practices, emerging technologies and strategies that leverage plant-microbe interactions for improving crop yields, soil fertility, and overall agricultural sustainability.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100333"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibacterial activity and impact on keratinocyte cell growth of Cutibacterium acnes bacteriophages in a Cutibacterium acnes IA1- colonized keratinocyte model
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100356
Juan C Farfán-Esquivel , María Victoria Gutiérrez , Alejandro Ondo-Méndez , John M González , Martha J Vives-Flórez
Acne is an inflammatory disease in which microbial disbalance is represented by an augmented population of phylotype IA1 of Cutibacterium acnes. Various treatments for acne can cause side effects, and it has been reported that C. acnes is resistant to prescribed antibiotics. Phage therapy has been proposed as an alternative treatment for acne, given its species-specificity to kill bacteria, its relative innocuity, and its potential to manage antibiotic-resistant pathogens. Moreover, bacteriophages (phages) may modulate the microbiota and immune responses. Some studies have shown the potential use of phages in the treatment of acne. Nevertheless, the capacity to specifically reduce phylotype IA1 and the effect of phage treatment on skin cells are poorly understood. We assessed the capacity of phages to clear C. acnes IA1 and their effects on cell cytotoxicity and growth in HEKa cells- C. acnes IA1 co-culture. Phylotypes IA1 and IB had similar effects on HEKa cells, causing cytotoxicity and diminishing cell growth. Nevertheless, IA1 caused a higher impact on cell doubling time by increasing it 1.8 times more than cell growth control group. Even though there are no phages IA1-specific, we found phages that have a diminished effect on other phylotypes not related to acne. Phage treatment in general reduced IA1-caused cytotoxicity, with differences in efficacy among phages. In addition, phage purification was necessary to restore metabolic activity and growth of HEKa. Overall, phage evaluation as a therapeutic alternative should include phage-bacteria interactions and their impact on skin cells because of the differences that each phage can exhibit.
{"title":"Antibacterial activity and impact on keratinocyte cell growth of Cutibacterium acnes bacteriophages in a Cutibacterium acnes IA1- colonized keratinocyte model","authors":"Juan C Farfán-Esquivel ,&nbsp;María Victoria Gutiérrez ,&nbsp;Alejandro Ondo-Méndez ,&nbsp;John M González ,&nbsp;Martha J Vives-Flórez","doi":"10.1016/j.crmicr.2025.100356","DOIUrl":"10.1016/j.crmicr.2025.100356","url":null,"abstract":"<div><div>Acne is an inflammatory disease in which microbial disbalance is represented by an augmented population of phylotype IA<sub>1</sub> of <em>Cutibacterium acnes</em>. Various treatments for acne can cause side effects, and it has been reported that <em>C. acnes</em> is resistant to prescribed antibiotics. Phage therapy has been proposed as an alternative treatment for acne, given its species-specificity to kill bacteria, its relative innocuity, and its potential to manage antibiotic-resistant pathogens. Moreover, bacteriophages (phages) may modulate the microbiota and immune responses. Some studies have shown the potential use of phages in the treatment of acne. Nevertheless, the capacity to specifically reduce phylotype IA<sub>1</sub> and the effect of phage treatment on skin cells are poorly understood. We assessed the capacity of phages to clear <em>C. acnes</em> IA<sub>1</sub> and their effects on cell cytotoxicity and growth in HEKa cells<em>- C. acnes</em> IA<sub>1</sub> co-culture. Phylotypes IA<sub>1</sub> and IB had similar effects on HEKa cells, causing cytotoxicity and diminishing cell growth. Nevertheless, IA<sub>1</sub> caused a higher impact on cell doubling time by increasing it 1.8 times more than cell growth control group. Even though there are no phages IA<sub>1</sub>-specific, we found phages that have a diminished effect on other phylotypes not related to acne. Phage treatment in general reduced IA<sub>1</sub>-caused cytotoxicity, with differences in efficacy among phages. In addition, phage purification was necessary to restore metabolic activity and growth of HEKa. Overall, phage evaluation as a therapeutic alternative should include phage-bacteria interactions and their impact on skin cells because of the differences that each phage can exhibit.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100356"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste 利用农业工业废弃物,酵母介导深层发酵生产皂角脂及其特性研究。
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100334
Aishwary Purohit , Amar Jyoti Das , Debashish Ghosh
The challenges of pollution and agro-industrial waste management have led to the development of bioconversion techniques to transform these wastes into valuable products. This has increased the focus on the sustainable and cost-efficient production of biosurfactants from agro-industrial waste. Hence, the present study investigates the production of sophorolipid biosurfactants using the yeast strain Rhodotorula mucilaginosa IIPL32 under submerged fermentation, employing sugarcane bagasse hydrolysate—a renewable, low-cost agro-industrial waste as the feedstock. By systematically optimizing strain adaptation, medium composition, and scaling up the process from shake flasks to a bioreactor, a maximum sophorolipid yield of 2.6 ± 0.21 g/L was achieved. Extensive characterization was conducted, encompassing emulsification index (54 %), surface tension reduction, and several chemical analyses (anthrone, iodine, saponification, lipid solubility). Advanced structural elucidation techniques such as Fourier-transform infrared (FTIR) spectroscopy, liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) spectroscopy were employed for structural confirmation of the extracted biosurfactant. FTIR spectroscopy identified characteristic functional groups, while LC-MS revealed distinct sophorolipid congeners with varying lipid chain lengths and acetylation. NMR spectroscopy corroborated the presence of disaccharide and fatty acid components, indicating the extracted biosurfactant might be sophorolipid. This study underscores the feasibility of utilizing agro-industrial waste for the eco-friendly production of sophorolipid biosurfactants and provides detailed insights into their structural features, highlighting their potential applications across diverse fields such as pharmaceuticals, cosmetics, and environmental remediation.
污染和农工废物管理的挑战导致了生物转化技术的发展,将这些废物转化为有价值的产品。这使人们更加重视从农业工业废物中可持续和经济高效地生产生物表面活性剂。因此,本研究以甘蔗甘蔗渣水解物——一种可再生的、低成本的农业工业废弃物为原料,利用酵母菌株粘液红酵母IIPL32进行深层发酵生产槐脂类生物表面活性剂。通过系统地优化菌株适应、培养基组成,并将工艺从摇瓶扩展到生物反应器,获得了2.6±0.21 g/L的最高皂荚脂产量。进行了广泛的表征,包括乳化指数(54%),表面张力降低,以及一些化学分析(蒽酮,碘,皂化,脂溶性)。采用傅里叶变换红外(FTIR)光谱、液相色谱-质谱(LC-MS)和核磁共振(NMR)等先进的结构解析技术对提取的生物表面活性剂进行结构鉴定。FTIR光谱鉴定出特征官能团,而LC-MS显示出具有不同脂链长度和乙酰化程度的独特苦参脂同源物。核磁共振证实了双糖和脂肪酸成分的存在,表明提取的生物表面活性剂可能是槐脂。本研究强调了利用农业工业废弃物环保生产皂荚脂生物表面活性剂的可行性,并提供了其结构特征的详细见解,突出了其在制药,化妆品和环境修复等多个领域的潜在应用。
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引用次数: 0
Legionella pneumophila subverts the antioxidant defenses of its amoeba host Acanthamoeba castellanii
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100338
Alban Hay , Willy Aucher , Romain Pigeault , Joanne Bertaux , Alexandre Crépin , Quentin Blancart Remaury , Yann Héchard , Ascel Samba-Louaka , Romain Villéger
Legionella pneumophila, the causative agent of Legionnaires' disease, interacts in the environment with free-living amoebae that serve as replicative niches for the bacteria. Among these amoebae, Acanthamoeba castellanii is a natural host in water networks and a model commonly used to study the interaction between L. pneumophila and its host. However, certain crucial aspects of this interaction remain unclear. One such aspect is the role of oxidative stress, with studies focusing on reactive oxygen species (ROS) production by the host and putting less emphasis on the involvement of the host's antioxidant defenses during the infectious process. In this study, we propose to examine the consequences of infection with L. pneumophila wild-type or with an isogenic ΔdotA mutant strain, which is unable to replicate intracellularly, on A. castellanii. For this purpose, we looked at the host ROS levels, host antioxidant defense transcripts, and metabolites linked to the amoeba's antioxidant defenses. It is known that L. pneumophila WT can block the activation of NADPH oxidase as soon as it enters the macrophage and suppress ROS production compared to ΔdotA mutant strain. In addition, it has been shown in macrophages that L. pneumophila WT decreases ROS at 24 h p.i.; here we confirm this result in amoebae and suggest that this decrease could be partly explained by L. pneumophila differentially regulated host antioxidant defense transcripts at 6 h p.i.. We also explored the metabolome of A. castellanii infected or not with L. pneumophila. Among the 617 metabolites identified, four with reduced abundances during infection may be involved in antioxidant responses. This study suggests that L. pneumophila could hijack the host's antioxidant defenses during its replication to maintain a reduced level of ROS.
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引用次数: 0
期刊
Current Research in Microbial Sciences
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