首页 > 最新文献

Current Research in Microbial Sciences最新文献

英文 中文
Revalorization of degraded maguey pulquero substrate for Lycopersicon esculentum germination 对降解的 maguey pulquero 基质进行再资源化,促进茄果类植物发芽
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100283
B.S. Velázquez-De Lucio , E.M. Hernández-Domínguez , M.P. Falcón-León , A. Téllez-Jurado , J Álvarez-Cervantes
The bagasse of Agave salmiana (maguey pulquero) is a residue generated during the exploitation of the plant to obtain pulque, inulin, honey, etc. Due to its chemical composition, it can be used for the cultivation of fungi of the Pleurotus genus and the subsequent use of the degraded material "degraded substrate (DS)" as a support for the germination of vegetables. The objective of the study was to characterize the bagasse of maguey pulquero biodegraded by Pleurotus djamor as a new perspective in its value chain, and subsequent use for the germination of Lycopersicon esculetum (tomato). The DS was recovered at 60 d from the P. djamor culture, characterized physicochemically and the conformation of the plant tissue was observed by scanning electron microscopy. The DS showed a decrease in protein (4.8–3.3 %) content and fibrous fraction (54–36 %), but dry matter digestibility increased from 47 to 71 %; in addition, changes in mineral composition were observed, mainly in calcium concentration (6 %). Due to its composition, it is possible to revalue DS in the germination of L. esculetum to reduce the use of peat moss (commercial peat). The results show that up to 25 % of maguey DS mixed with 75 % peat moss can be used (25:75), reaching a germination percentage of 85 % and increasing the seedling emergence speed index from 0.96 – 1.25. Concluding that it is possible to implement a circular strategy in which agave bagasse is used for mushroom cultivation and the subsequent recovery of the spent substrate for tomato germination.
龙舌兰(maguey pulquero)的蔗渣是在利用龙舌兰植物获取豆浆、菊粉、蜂蜜等过程中产生的残留物。由于其化学成分,它可用于培植龙舌兰属真菌,随后将降解材料 "降解基质(DS)"用作蔬菜发芽的支持物。这项研究的目的是描述由 Pleurotus djamor 生物降解的 maguey pulquero 甘蔗渣的特征,作为其价值链中的一个新视角,并随后用于番茄(Lycopersicon esculetum)的发芽。从 P. djamor 培养物中 60 天后回收了 DS,对其进行了理化鉴定,并通过扫描电子显微镜观察了植物组织的构象。DS 蛋白质(4.8%-3.3%)含量和纤维部分(54%-36%)有所下降,但干物质消化率从 47% 提高到 71%;此外,还观察到矿物质成分的变化,主要是钙浓度(6%)的变化。根据其成分,有可能在 L. esculetum 的发芽过程中重估 DS 的价值,以减少泥炭藓(商用泥炭)的使用。结果表明,可以使用 25% 的岩浆草 DS 与 75% 的泥炭藓混合(25:75),使发芽率达到 85%,出苗速度指数从 0.96 提高到 1.25。结论是可以实施一种循环战略,即利用龙舌兰甘蔗渣栽培蘑菇,然后回收废基质用于番茄发芽。
{"title":"Revalorization of degraded maguey pulquero substrate for Lycopersicon esculentum germination","authors":"B.S. Velázquez-De Lucio ,&nbsp;E.M. Hernández-Domínguez ,&nbsp;M.P. Falcón-León ,&nbsp;A. Téllez-Jurado ,&nbsp;J Álvarez-Cervantes","doi":"10.1016/j.crmicr.2024.100283","DOIUrl":"10.1016/j.crmicr.2024.100283","url":null,"abstract":"<div><div>The bagasse of <em>Agave salmiana</em> (maguey pulquero) is a residue generated during the exploitation of the plant to obtain pulque, inulin, honey, etc. Due to its chemical composition, it can be used for the cultivation of fungi of the <em>Pleurotus</em> genus and the subsequent use of the degraded material \"degraded substrate (DS)\" as a support for the germination of vegetables. The objective of the study was to characterize the bagasse of maguey pulquero biodegraded by <em>Pleurotus djamor</em> as a new perspective in its value chain, and subsequent use for the germination of <em>Lycopersicon esculetum</em> (tomato). The DS was recovered at 60 d from the <em>P. djamor</em> culture, characterized physicochemically and the conformation of the plant tissue was observed by scanning electron microscopy. The DS showed a decrease in protein (4.8–3.3 %) content and fibrous fraction (54–36 %), but dry matter digestibility increased from 47 to 71 %; in addition, changes in mineral composition were observed, mainly in calcium concentration (6 %). Due to its composition, it is possible to revalue DS in the germination of L. <em>esculetum</em> to reduce the use of peat moss (commercial peat). The results show that up to 25 % of maguey DS mixed with 75 % peat moss can be used (25:75), reaching a germination percentage of 85 % and increasing the seedling emergence speed index from 0.96 – 1.25. Concluding that it is possible to implement a circular strategy in which agave bagasse is used for mushroom cultivation and the subsequent recovery of the spent substrate for tomato germination.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100283"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a luminescence-based method for measuring West Nile Virus MTase activity and its application to screen for antivirals 开发基于发光的西尼罗河病毒 MT 酶活性测量方法及其在筛选抗病毒药物中的应用
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100282
Alejandra Álvarez-Mínguez , Natalia del Río , Ana Belén-Blázquez , Elena Casanova , José-María Orduña , Patricia Camarero , Carolina Hurtado-Marcos , Carmen del Águila , María–Jesús Pérez-Pérez , Miguel A. Martín-Acebes , Rubén Agudo
West Nile virus (WNV) is a flavivirus responsible for causing febrile illness and severe neurological diseases, with an increasing impact on human health around the world. However, there is still no adequate therapeutic treatment available to struggle WNV infections. Therefore, there is an urgent need to develop new techniques to accelerate the discovery of drugs against this pathogen. The main protein implicated in the replication of WNV is the non-structural protein 5 (NS5). This multifunctional protein contains methyltransferase (MTase) activity involved in the capping formation at the 5′-end of RNA and the methylation of internal viral RNA residues, both functions being essential for viral processes, such as RNA translation and escape from the innate immune response.
We have developed a straightforward luminescence-based assay to monitor the MTase activity of the WNV NS5 protein with potential for high-throughput screening. We have validated this method as a sensitive and suitable assay for the identification of WNV MTase inhibitors assessing the inhibitory effect of the broad MTase inhibitor sinefungin, a natural nucleoside analog of the universal methyl donor S-adenosyl methionine (SAM). The screening of a small series of purine derivatives identified an adenosine derivative as a dose-dependent inhibitor of the MTase activity. The antiviral efficacy of this compound was further confirmed in WNV infections, displaying a measurable antiviral effect. This result supports the utility of this novel method for the screening of inhibitors against WNV MTase activity, which can be of special relevance to the discovery and development of therapeutics against WNV.
西尼罗河病毒(WNV)是一种黄病毒,可引起发热性疾病和严重的神经系统疾病,对全球人类健康的影响与日俱增。然而,目前仍没有适当的治疗方法来抗击 WNV 感染。因此,迫切需要开发新技术,加快发现抗这种病原体的药物。与 WNV 复制有关的主要蛋白质是非结构蛋白 5(NS5)。这种多功能蛋白含有甲基转移酶(MTase)活性,参与 RNA 5′ 端封盖的形成和病毒内部 RNA 残基的甲基化,这两种功能对于病毒过程(如 RNA 翻译和逃避先天性免疫反应)至关重要。我们已经验证了这种方法是一种灵敏且适用于鉴定 WNV MT 酶抑制剂的检测方法,并评估了广谱 MT 酶抑制剂正银霉素的抑制作用,正银霉素是通用甲基供体 S-腺苷蛋氨酸(SAM)的天然核苷类似物。通过对一小系列嘌呤衍生物的筛选,发现一种腺苷衍生物是 MT 酶活性的剂量依赖性抑制剂。该化合物的抗病毒功效在 WNV 感染中得到了进一步证实,显示出了可测量的抗病毒效果。这一结果证明了这种新方法在筛选 WNV MT 酶活性抑制剂方面的实用性,对发现和开发针对 WNV 的疗法具有特殊意义。
{"title":"Development of a luminescence-based method for measuring West Nile Virus MTase activity and its application to screen for antivirals","authors":"Alejandra Álvarez-Mínguez ,&nbsp;Natalia del Río ,&nbsp;Ana Belén-Blázquez ,&nbsp;Elena Casanova ,&nbsp;José-María Orduña ,&nbsp;Patricia Camarero ,&nbsp;Carolina Hurtado-Marcos ,&nbsp;Carmen del Águila ,&nbsp;María–Jesús Pérez-Pérez ,&nbsp;Miguel A. Martín-Acebes ,&nbsp;Rubén Agudo","doi":"10.1016/j.crmicr.2024.100282","DOIUrl":"10.1016/j.crmicr.2024.100282","url":null,"abstract":"<div><div>West Nile virus (WNV) is a flavivirus responsible for causing febrile illness and severe neurological diseases, with an increasing impact on human health around the world. However, there is still no adequate therapeutic treatment available to struggle WNV infections. Therefore, there is an urgent need to develop new techniques to accelerate the discovery of drugs against this pathogen. The main protein implicated in the replication of WNV is the non-structural protein 5 (NS5). This multifunctional protein contains methyltransferase (MTase) activity involved in the capping formation at the 5′-end of RNA and the methylation of internal viral RNA residues, both functions being essential for viral processes, such as RNA translation and escape from the innate immune response.</div><div>We have developed a straightforward luminescence-based assay to monitor the MTase activity of the WNV NS5 protein with potential for high-throughput screening. We have validated this method as a sensitive and suitable assay for the identification of WNV MTase inhibitors assessing the inhibitory effect of the broad MTase inhibitor sinefungin, a natural nucleoside analog of the universal methyl donor S-adenosyl methionine (SAM). The screening of a small series of purine derivatives identified an adenosine derivative as a dose-dependent inhibitor of the MTase activity. The antiviral efficacy of this compound was further confirmed in WNV infections, displaying a measurable antiviral effect. This result supports the utility of this novel method for the screening of inhibitors against WNV MTase activity, which can be of special relevance to the discovery and development of therapeutics against WNV.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100282"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ibrexafungerp: A narrative overview Ibrexafungerp:叙述性概述
Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100245
L'Emir Wassim El Ayoubi , Fatima Allaw , Elie Moussa , Souha S. Kanj

Ibrexafungerp (IBX) is a new antifungal drug that recently entered the antifungal landscape. It disrupts fungal cell wall synthesis by non-competitive inhibition of the β-(1,3)-D-glucan (BDG) synthase enzyme. It has demonstrated activity against a range of pathogens including Candida and Aspergillus spp., as well as retaining its activity against azole-resistant and echinocandin-resistant strains. It also exhibits anti-biofilm properties. Pharmacokinetic (PK) studies revealed favorable bioavailability, high protein binding, and extensive tissue distribution with a low potential for CYP-mediated drug interactions. It is characterized by the same mechanism of action of echinocandins with limited cross-resistance with other antifungal agents. Resistance to this drug can arise from mutations in the FKS genes, primarily FKS2 mutations in Nakaseomyces glabrata. In vivo, IBX was found to be effective in murine models of invasive candidiasis (IC) and invasive pulmonary aspergillosis (IPA). It also showed promising results in preventing and treating Pneumocystis jirovecii infections. Clinical trials showed that IBX was effective and non-inferior to fluconazole in treating vulvovaginal candidiasis (VVC), including complicated cases, as well as in preventing its recurrence. These trials positioned it as a Food and Drug Administration (FDA)-approved option for the treatment and prophylaxis of VVC. Trials showed comparable responses to standard-of-care in IC, with favorable preliminary results in C. auris infections in terms of efficacy and tolerability as well as in refractory cases of IC. Mild adverse reactions have been reported including gastrointestinal symptoms. Overall, IBX represents a significant addition to the antifungal armamentarium, with its unique action, spectrum of activity, and encouraging clinical trial results warranting further investigation.

Ibrexafungerp(IBX)是最近进入抗真菌领域的一种新型抗真菌药物。它通过非竞争性抑制β-(1,3)-D-葡聚糖(BDG)合成酶来破坏真菌细胞壁的合成。它对包括念珠菌和曲霉菌属在内的一系列病原体都具有活性,并对耐唑类和耐棘白菌素菌株保持活性。它还具有抗生物膜特性。药代动力学(PK)研究显示,该药物具有良好的生物利用度、高蛋白结合力和广泛的组织分布,且由 CYP 介导的药物相互作用可能性较低。它与棘白菌素的作用机制相同,与其他抗真菌药物的交叉耐药性有限。对这种药物的耐药性可能源于 FKS 基因的突变,主要是玻璃酸中酵母菌中 FKS2 基因的突变。在体内,IBX 对侵袭性念珠菌病(IC)和侵袭性肺曲霉病(IPA)的小鼠模型有效。它在预防和治疗肺孢子虫感染方面也显示出良好的效果。临床试验表明,IBX 在治疗外阴阴道念珠菌病(VVC)(包括复杂病例)和预防复发方面的效果并不亚于氟康唑。这些试验使其成为美国食品药品管理局(FDA)批准的治疗和预防外阴阴道念珠菌病的选择。试验结果表明,该药对 IC 的疗效与标准疗法相当,对 C. auris 感染的疗效和耐受性以及 IC 的难治性病例均取得了良好的初步结果。也有轻微不良反应的报道,包括胃肠道症状。总之,IBX 是抗真菌药物库中的重要一员,其独特的作用、活性范围和令人鼓舞的临床试验结果值得进一步研究。
{"title":"Ibrexafungerp: A narrative overview","authors":"L'Emir Wassim El Ayoubi ,&nbsp;Fatima Allaw ,&nbsp;Elie Moussa ,&nbsp;Souha S. Kanj","doi":"10.1016/j.crmicr.2024.100245","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100245","url":null,"abstract":"<div><p>Ibrexafungerp (IBX) is a new antifungal drug that recently entered the antifungal landscape. It disrupts fungal cell wall synthesis by non-competitive inhibition of the β-(1,3)-D-glucan (BDG) synthase enzyme. It has demonstrated activity against a range of pathogens including <em>Candida</em> and <em>Aspergillus</em> spp., as well as retaining its activity against azole-resistant and echinocandin-resistant strains. It also exhibits anti-biofilm properties. Pharmacokinetic (PK) studies revealed favorable bioavailability, high protein binding, and extensive tissue distribution with a low potential for CYP-mediated drug interactions. It is characterized by the same mechanism of action of echinocandins with limited cross-resistance with other antifungal agents. Resistance to this drug can arise from mutations in the <em>FKS</em> genes, primarily <em>FKS2</em> mutations in <em>Nakaseomyces glabrata</em>. In vivo, IBX was found to be effective in murine models of invasive candidiasis (IC) and invasive pulmonary aspergillosis (IPA). It also showed promising results in preventing and treating <em>Pneumocystis jirovecii</em> infections. Clinical trials showed that IBX was effective and non-inferior to fluconazole in treating vulvovaginal candidiasis (VVC), including complicated cases, as well as in preventing its recurrence. These trials positioned it as a Food and Drug Administration (FDA)-approved option for the treatment and prophylaxis of VVC. Trials showed comparable responses to standard-of-care in IC, with favorable preliminary results in <em>C. auris</em> infections in terms of efficacy and tolerability as well as in refractory cases of IC. Mild adverse reactions have been reported including gastrointestinal symptoms. Overall, IBX represents a significant addition to the antifungal armamentarium, with its unique action, spectrum of activity, and encouraging clinical trial results warranting further investigation.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100245"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000270/pdfft?md5=c5569e8a01322f3810b939d41b0f2226&pid=1-s2.0-S2666517424000270-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141243895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells 外膜囊泡特异性脂蛋白促进牙龈卟啉单胞菌在红细胞上聚集
Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100249
Christina M. Rothenberger , Manda Yu , Hey-Min Kim , Yee-Wai Cheung , Yi-Wei Chang , Mary Ellen Davey

Porphyromonas gingivalis uses a variety of mechanisms to actively interact with and promote the hydrolysis of red blood cells (RBCs) to obtain iron in the form of heme. In this study, we investigated the function of lipoprotein PG1881 which was previously shown to be up-regulated during subsurface growth and selectively enriched on outer membrane vesicles (OMVs). Our results show that wildtype strain W83 formed large aggregates encompassing RBCs whereas the PG1881 deletion mutant remained predominately as individual cells. Using a PG1881 antibody, immunofluorescence revealed that the wildtype strain's aggregation to RBCs involves an extracellular matrix enriched with PG1881. Our findings discover that RBCs elicit cell aggregation and matrix formation by P. gingivalis and that this process is promoted by an OMV-specific lipoprotein. We propose this strategy is advantageous for nutrient acquisition as well as dissemination from the oral cavity and survival of this periodontal pathogen.

牙龈卟啉单胞菌(Porphyromonas gingivalis)利用多种机制积极与红细胞(RBC)相互作用并促进其水解,以获取血红素形式的铁。在本研究中,我们对脂蛋白 PG1881 的功能进行了研究,之前的研究表明,脂蛋白 PG1881 在表面下生长过程中会上调,并选择性地富集在外膜囊泡(OMVs)上。我们的结果表明,野生型菌株 W83 形成了包含 RBC 的大型聚集体,而 PG1881 缺失突变体则主要是单个细胞。使用 PG1881 抗体,免疫荧光显示野生型菌株与 RBC 的聚集涉及富含 PG1881 的细胞外基质。我们的研究结果发现,牙龈脓杆菌能通过 RBC 诱导细胞聚集和基质形成,而这一过程是由 OMV 特异性脂蛋白促进的。我们认为这种策略有利于牙龈脓疱梭菌从口腔中获取营养、传播和存活。
{"title":"An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells","authors":"Christina M. Rothenberger ,&nbsp;Manda Yu ,&nbsp;Hey-Min Kim ,&nbsp;Yee-Wai Cheung ,&nbsp;Yi-Wei Chang ,&nbsp;Mary Ellen Davey","doi":"10.1016/j.crmicr.2024.100249","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100249","url":null,"abstract":"<div><p><em>Porphyromonas gingivalis</em> uses a variety of mechanisms to actively interact with and promote the hydrolysis of red blood cells (RBCs) to obtain iron in the form of heme. In this study, we investigated the function of lipoprotein PG1881 which was previously shown to be up-regulated during subsurface growth and selectively enriched on outer membrane vesicles (OMVs). Our results show that wildtype strain W83 formed large aggregates encompassing RBCs whereas the PG1881 deletion mutant remained predominately as individual cells. Using a PG1881 antibody, immunofluorescence revealed that the wildtype strain's aggregation to RBCs involves an extracellular matrix enriched with PG1881. Our findings discover that RBCs elicit cell aggregation and matrix formation by <em>P. gingivalis</em> and that this process is promoted by an OMV-specific lipoprotein. We propose this strategy is advantageous for nutrient acquisition as well as dissemination from the oral cavity and survival of this periodontal pathogen.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100249"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000312/pdfft?md5=9c4453b76cfe73e58ed15aa05c51381d&pid=1-s2.0-S2666517424000312-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141324863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial diversity along the geothermal gradients: insights from the high-altitude Himalayan hot spring habitats of Sikkim 地热梯度上的细菌多样性:锡金高海拔喜马拉雅温泉栖息地的启示
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100310
Santosh Kumar , Sayak Das , Namrata Jiya , Avinash Sharma , Chirantan Saha , Prayatna Sharma , Sonia Tamang , Nagendra Thakur
Geothermal habitats present a unique opportunity to study microbial adaptation to varying temperature conditions. In such environments, distinct temperature gradients foster diverse microbial communities, each adapted to its optimal niche. However, the complex dynamics of bacterial populations in across these gradients high-altitude hot springs remain largely unexplored. We hypothesize that temperature is a primary driver of microbial diversity, and bacterial richness peaks at intermediate temperatures. To investigate this, we analysed bacterial diversity using 16S rRNA amplicon sequencing across three temperature regions: hot region of 56–65 °C (hot spring), warm region of 35–37 °C (path carrying hot spring water to the river), and cold region of 4–7 °C (river basin). Our findings showed that Bacillota was the most abundant phylum (45.51 %), followed by Pseudomonadota (32.81 %) and Actinomycetota (7.2 %). Bacillota and Chloroflexota flourished in the hot and warm regions, while Pseudomonadota thrived in cooler areas. Core microbiome analysis indicated that species richness was highest in the warm region, declining in both cold and hot regions. Interestingly, an anomaly was observed with Staphylococcus, which was more abundant in cases where ponds were used for bathing and recreation. In contrast, Clostridium was mostly found in cold regions, likely due to its viability in soil and ability to remain dormant as a spore-forming bacterium. The warm region showed the highest bacterial diversity, while richness decreased in both cold and hot regions. This highlights the temperature-dependent nature of microbial communities, with optimal diversity in moderate thermal conditions. The study offers new insights into microbial dynamics in high-altitude geothermal systems.
地热栖息地为研究微生物对不同温度条件的适应性提供了一个独特的机会。在这种环境中,不同的温度梯度促进了微生物群落的多样性,每个群落都适应其最佳生态位。然而,这些梯度高海拔温泉中细菌种群的复杂动态在很大程度上仍未得到探索。我们假设,温度是微生物多样性的主要驱动因素,而细菌的丰富程度在中间温度达到顶峰。为了研究这一点,我们使用 16S rRNA 扩增子测序分析了三个温度区域的细菌多样性:56-65 ℃ 的高温区(温泉)、35-37 ℃ 的温暖区(温泉水流向河流的路径)和 4-7 ℃ 的低温区(河流流域)。研究结果表明,芽孢杆菌科(Bacillota)的数量最多(45.51 %),其次是假单胞菌科(Pseudomonadota)(32.81 %)和放线菌科(Actinomycetota)(7.2 %)。芽孢杆菌和绿僵菌在炎热和温暖地区生长旺盛,而假单胞菌在凉爽地区生长旺盛。核心微生物组分析表明,温暖地区的物种丰富度最高,而寒冷和炎热地区的物种丰富度均有所下降。有趣的是,葡萄球菌出现了异常,在池塘用于沐浴和娱乐的情况下,葡萄球菌的数量更多。相比之下,梭状芽孢杆菌主要出现在寒冷地区,这可能是由于其在土壤中的生存能力以及作为孢子形成菌保持休眠的能力。温暖地区的细菌多样性最高,而寒冷和炎热地区的丰富度都有所下降。这凸显了微生物群落的温度依赖性,在适度的热量条件下具有最佳的多样性。这项研究为了解高海拔地热系统中的微生物动态提供了新的视角。
{"title":"Bacterial diversity along the geothermal gradients: insights from the high-altitude Himalayan hot spring habitats of Sikkim","authors":"Santosh Kumar ,&nbsp;Sayak Das ,&nbsp;Namrata Jiya ,&nbsp;Avinash Sharma ,&nbsp;Chirantan Saha ,&nbsp;Prayatna Sharma ,&nbsp;Sonia Tamang ,&nbsp;Nagendra Thakur","doi":"10.1016/j.crmicr.2024.100310","DOIUrl":"10.1016/j.crmicr.2024.100310","url":null,"abstract":"<div><div>Geothermal habitats present a unique opportunity to study microbial adaptation to varying temperature conditions. In such environments, distinct temperature gradients foster diverse microbial communities, each adapted to its optimal niche. However, the complex dynamics of bacterial populations in across these gradients high-altitude hot springs remain largely unexplored. We hypothesize that temperature is a primary driver of microbial diversity, and bacterial richness peaks at intermediate temperatures. To investigate this, we analysed bacterial diversity using 16S rRNA amplicon sequencing across three temperature regions: hot region of 56–65 °C (hot spring), warm region of 35–37 °C (path carrying hot spring water to the river), and cold region of 4–7 °C (river basin). Our findings showed that Bacillota was the most abundant phylum (45.51 %), followed by Pseudomonadota (32.81 %) and Actinomycetota (7.2 %). Bacillota and Chloroflexota flourished in the hot and warm regions, while Pseudomonadota thrived in cooler areas. Core microbiome analysis indicated that species richness was highest in the warm region, declining in both cold and hot regions. Interestingly, an anomaly was observed with <em>Staphylococcus</em>, which was more abundant in cases where ponds were used for bathing and recreation. In contrast, <em>Clostridium</em> was mostly found in cold regions, likely due to its viability in soil and ability to remain dormant as a spore-forming bacterium. The warm region showed the highest bacterial diversity, while richness decreased in both cold and hot regions. This highlights the temperature-dependent nature of microbial communities, with optimal diversity in moderate thermal conditions. The study offers new insights into microbial dynamics in high-altitude geothermal systems.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100310"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Subinhibitory concentrations of meropenem stimulate membrane vesicle production and modulate immune response in Bacteroides fragilis infection 抑菌浓度以下的美罗培南可刺激膜泡生成并调节脆弱拟杆菌感染的免疫反应
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100294
Saniya Kozhakhmetova , Ayazhan Bekbayeva , Elena Zholdybayeva , Tatyana Krivoruchko , Natalya Dashevskaya , Zhanel Mukhanbetzhanova , Elizaveta Vinogradova , Almagul Kushugulova , Samat Kozhakhmetov
This study explores an adaptation mechanism of Bacteroides fragilis to subinhibitory concentrations of meropenem, characterized by an alteration in the production of membrane vesicles (MVs) and modulation of the host inflammatory response. Using a rat model of infection, we demonstrated a significant increase in the size of MVs accompanied by a nonsignificant increase in their number in the meropenem-treated group compared to the infected control. Both infected groups showed significantly altered hematological parameters and shifts in monocyte on day 8 (average increase of 21.5 %). At the same time, significant changes in neutrophils (decrease by 26 %) and eosinophils (increase by 3 %) were observed only in the infected group but not in the infected meropenem-treated group. On day 16, increased macrophage activation, neovascularization, and fibrosis were observed in the tissues of the antibiotic-treated group. Immunological profile analysis revealed a slight increase in the levels of pro-inflammatory cytokines (IL-5, IL-6, IFN-γ and G-CSF) on day 8 of the experiment, followed by a sharp decrease on day 16 in both infected groups compared to the negative control. At the same time, network analysis of correlations between these immunological factors showed complex changes in response to subinhibitory concentrations of meropenem. The bacterial load did not differ between the infected groups on days 8 and 16, but only in the meropenem-free group a significant decrease in the number of bacteria was observed on day 16 in all samples. These findings suggest that subinhibitory antibiotic concentrations can influence the pathophysiological progression of B. fragilis infection, modulating both the bacterial response and the host immune reaction, potentially leading to a more complex and chronic disease course.
本研究探讨了脆弱拟杆菌对亚抑制浓度美罗培南的适应机制,这种机制的特点是膜囊泡 (MV) 的产生发生了变化并调节了宿主的炎症反应。通过大鼠感染模型,我们发现与受感染的对照组相比,美罗培南处理组的膜囊泡体积显著增大,但数量却无明显增加。两个感染组在第 8 天都显示出明显的血液学参数变化和单核细胞变化(平均增加 21.5%)。同时,仅在感染组观察到中性粒细胞(减少 26%)和嗜酸性粒细胞(增加 3%)的明显变化,而在感染的美罗培南处理组则未观察到。第 16 天,在抗生素治疗组的组织中观察到巨噬细胞活化、新生血管和纤维化增加。免疫谱分析显示,与阴性对照组相比,两个感染组的促炎细胞因子(IL-5、IL-6、IFN-γ 和 G-CSF)水平在实验第 8 天略有上升,随后在第 16 天急剧下降。同时,这些免疫因子之间的相关网络分析显示了对亚抑制浓度美罗培南反应的复杂变化。感染组之间在第 8 天和第 16 天的细菌量没有差异,但只有不含美罗培南的感染组在第 16 天的所有样本中观察到细菌数量显著减少。这些研究结果表明,亚抑制浓度的抗生素可影响脆弱拟杆菌感染的病理生理进展,调节细菌反应和宿主免疫反应,从而可能导致更复杂的慢性病程。
{"title":"Subinhibitory concentrations of meropenem stimulate membrane vesicle production and modulate immune response in Bacteroides fragilis infection","authors":"Saniya Kozhakhmetova ,&nbsp;Ayazhan Bekbayeva ,&nbsp;Elena Zholdybayeva ,&nbsp;Tatyana Krivoruchko ,&nbsp;Natalya Dashevskaya ,&nbsp;Zhanel Mukhanbetzhanova ,&nbsp;Elizaveta Vinogradova ,&nbsp;Almagul Kushugulova ,&nbsp;Samat Kozhakhmetov","doi":"10.1016/j.crmicr.2024.100294","DOIUrl":"10.1016/j.crmicr.2024.100294","url":null,"abstract":"<div><div>This study explores an adaptation mechanism of <em>Bacteroides fragilis</em> to subinhibitory concentrations of meropenem, characterized by an alteration in the production of membrane vesicles (MVs) and modulation of the host inflammatory response. Using a rat model of infection, we demonstrated a significant increase in the size of MVs accompanied by a nonsignificant increase in their number in the meropenem-treated group compared to the infected control. Both infected groups showed significantly altered hematological parameters and shifts in monocyte on day 8 (average increase of 21.5 %). At the same time, significant changes in neutrophils (decrease by 26 %) and eosinophils (increase by 3 %) were observed only in the infected group but not in the infected meropenem-treated group. On day 16, increased macrophage activation, neovascularization, and fibrosis were observed in the tissues of the antibiotic-treated group. Immunological profile analysis revealed a slight increase in the levels of pro-inflammatory cytokines (IL-5, IL-6, IFN-γ and G-CSF) on day 8 of the experiment, followed by a sharp decrease on day 16 in both infected groups compared to the negative control. At the same time, network analysis of correlations between these immunological factors showed complex changes in response to subinhibitory concentrations of meropenem. The bacterial load did not differ between the infected groups on days 8 and 16, but only in the meropenem-free group a significant decrease in the number of bacteria was observed on day 16 in all samples. These findings suggest that subinhibitory antibiotic concentrations can influence the pathophysiological progression of <em>B. fragilis</em> infection, modulating both the bacterial response and the host immune reaction, potentially leading to a more complex and chronic disease course.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100294"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic insights of a native bacterial consortium for wheat production sustainability 从基因组学角度洞察本土细菌联合体对小麦生产可持续性的影响
Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100230
Marisol Ayala Zepeda , Valeria Valenzuela Ruiz , Fannie Isela Parra Cota , Cristina Chinchilla-Soto , Eulogio de la Cruz Torres , María Itria Ibba , María Isabel Estrada Alvarado , Sergio de los Santos Villalobos

The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium (Bacillus cabrialesii subsp. cabrialesii TE3T, Priestia megaterium TRQ8, and Bacillus paralicheniformis TRQ65), through bioinformatic analysis, and to quantify the impact of its inoculation on NUE (measured through 15N-isotopic techniques), grain yield, and grain quality of durum wheat variety CIRNO C2008 grown under three doses of urea (0, 120, and 240 kg N ha−1) during two consecutive agricultural cycles in the Yaqui Valley, Mexico. The inoculation of the bacterial consortium (BC) to the wheat crop, at a total N concentration of 123–225 kg N ha−1 increased crop productivity and maintained grain quality, resulting in a yield increase of 1.1 ton ha−1 (6.0 vs. 7.1 ton ha−1, 0 kg N ha−1 added, 123 kg N ha−1 in the soil) and of 2.0 ton ha−1 (5.9 vs. 7.9 ton ha−1, 120 kg N ha−1 added, 104 kg N ha−1 in the soil) compared to the uninoculated controls at the same doses of N. The genomic bioinformatic analysis of the studied strains showed a great number of biofertilization-related genes regarding N and Fe acquisition, P assimilation, CO2 fixation, Fe, P, and K solubilization, with important roles in agroecosystems, as well as genes related to the production of siderophores and stress response. A positive effect of the BC on NUE at the studied initial N content (123 and 104 kg N ha−1) was not observed. Nevertheless, increases of 14 % and 12.5 % on NUE (whole plant) were observed when 120 kg N ha−1 was applied compared to when wheat was fully fertilized (240 kg N ha−1). This work represents a link between bioinformatic approaches of a native bacterial inoculant and the quantification of its impact on durum wheat.

使用促进植物生长的细菌作为生物接种剂是提高作物产量和质量以及改善植物肥料氮利用效率(NUE)的有力工具。本研究旨在对一种本地细菌(Bacillus cabrialesii subsp.cabrialesii TE3T、Priestia megaterium TRQ8 和 Bacillus paralicheniformis TRQ65),并量化其接种对墨西哥亚基河谷在两个连续农业周期内生长的硬质小麦品种 CIRNO C2008 的氮利用效率(通过 15N 同位素技术测量)、谷物产量和谷物品质的影响。在总氮浓度为 123-225 千克/公顷的条件下,给小麦作物接种细菌群(BC)可提高作物产量并保持谷物品质,从而使产量增加 1.1 吨/公顷(6.0 吨/公顷 vs. 7.1 吨/公顷,0 千克/公顷氮添加量,土壤中 123 千克/公顷氮添加量)和 2.0 吨/公顷(5.9 吨/公顷 vs. 7. 9 吨/公顷,120 千克/公顷氮添加量,土壤中 123 千克/公顷氮添加量)。对所研究菌株的基因组生物信息分析表明,有大量与生物肥料相关的基因,涉及氮和铁的获取、磷同化、二氧化碳固定、铁、磷和钾的增溶,这些基因在农业生态系统中发挥着重要作用,此外还有与产生苷元和应激反应相关的基因。在研究的初始氮含量(123 和 104 千克氮公顷-1)下,未观察到碱性催化还原剂对氮利用率的积极影响。然而,与小麦完全施肥(每公顷 240 千克氮)相比,施用每公顷 120 千克氮时,氮利用效率(全株)分别提高了 14% 和 12.5%。这项工作体现了本地细菌接种剂的生物信息学方法与量化其对硬粒小麦影响之间的联系。
{"title":"Genomic insights of a native bacterial consortium for wheat production sustainability","authors":"Marisol Ayala Zepeda ,&nbsp;Valeria Valenzuela Ruiz ,&nbsp;Fannie Isela Parra Cota ,&nbsp;Cristina Chinchilla-Soto ,&nbsp;Eulogio de la Cruz Torres ,&nbsp;María Itria Ibba ,&nbsp;María Isabel Estrada Alvarado ,&nbsp;Sergio de los Santos Villalobos","doi":"10.1016/j.crmicr.2024.100230","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100230","url":null,"abstract":"<div><p>The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium (<em>Bacillus cabrialesii</em> subsp. <em>cabrialesii</em> TE3<sup>T</sup>, <em>Priestia megaterium</em> TRQ8, and <em>Bacillus paralicheniformis</em> TRQ65), through bioinformatic analysis, and to quantify the impact of its inoculation on NUE (measured through <sup>15</sup>N-isotopic techniques), grain yield, and grain quality of durum wheat variety CIRNO C2008 grown under three doses of urea (0, 120, and 240 kg N ha<sup>−1</sup>) during two consecutive agricultural cycles in the Yaqui Valley, Mexico. The inoculation of the bacterial consortium (BC) to the wheat crop, at a total N concentration of 123–225 kg N ha<sup>−1</sup> increased crop productivity and maintained grain quality, resulting in a yield increase of 1.1 ton ha<sup>−1</sup> (6.0 vs. 7.1 ton ha<sup>−1</sup>, 0 kg N ha<sup>−1</sup> added, 123 kg N ha<sup>−1</sup> in the soil) and of 2.0 ton ha<sup>−1</sup> (5.9 vs. 7.9 ton ha<sup>−1</sup>, 120 kg N ha<sup>−1</sup> added, 104 kg N ha<sup>−1</sup> in the soil) compared to the uninoculated controls at the same doses of N. The genomic bioinformatic analysis of the studied strains showed a great number of biofertilization-related genes regarding N and Fe acquisition, P assimilation, CO<sub>2</sub> fixation, Fe, P, and K solubilization, with important roles in agroecosystems, as well as genes related to the production of siderophores and stress response. A positive effect of the BC on NUE at the studied initial N content (123 and 104 kg N ha<sup>−1</sup>) was not observed. Nevertheless, increases of 14 % and 12.5 % on NUE (whole plant) were observed when 120 kg N ha<sup>−1</sup> was applied compared to when wheat was fully fertilized (240 kg N ha<sup>−1</sup>). This work represents a link between bioinformatic approaches of a native bacterial inoculant and the quantification of its impact on durum wheat.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100230"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000129/pdfft?md5=87da0a38e8bd2da9662034dda18ad225&pid=1-s2.0-S2666517424000129-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140096320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A concise review on waste biomass valorization through thermochemical conversion 通过热化学转化实现废弃生物质增值的简明综述
Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100237
Naveen Chandra Joshi , Somya Sinha , Pooja Bhatnagar , Yogesh Nath , Bhavya Negi , Vinod Kumar , Prateek Gururani

Due to an increase in industrialization and urbanization, massive amounts of solid waste biomass are speedily accumulating in our environment, which poses several adverse effects on habitat and human health thus becoming a matter of discussion in the environmental community. With reference to the circular economy, continuous efforts have been put forward for setting up an organised management approach in combination with an efficient treatment technique for increasing the profitable utilization of solid waste. This review aims to provide a systematic discussion on the recent thermochemical technologies employed for converting waste biomass generated from different sources into valuable products like biochar, bio-oil, heat, energy and syngas. The article further focuses on a few important aspects of thermochemical conversion of waste biomass to useful products like technical factors affecting thermochemical processes, applications of by-products of thermochemical conversion, and biological pretreatment of waste biomass. The review assists interesting recent and scientific trends for boosting up the systematic management and valorization of solid waste through low-cost, efficient, environment-friendly and sustainable technologies.

由于工业化和城市化的发展,大量的固体废物生物质在我们的环境中迅速积累,对栖息地和人类健康造成了一些不利影响,从而成为环境界讨论的一个问题。在循环经济的背景下,人们不断努力建立有组织的管理方法,并结合高效的处理技术,以提高固体废物的利用率。本综述旨在系统地讨论近期采用的热化学技术,将不同来源产生的废弃生物质转化为有价值的产品,如生物炭、生物油、热能、能源和合成气。文章进一步关注了将废弃生物质热化学转化为有用产品的几个重要方面,如影响热化学过程的技术因素、热化学转化副产品的应用以及废弃生物质的生物预处理。文章通过低成本、高效率、环境友好和可持续发展的技术,为促进固体废物的系统管理和价值评估提供了有趣的最新科学趋势。
{"title":"A concise review on waste biomass valorization through thermochemical conversion","authors":"Naveen Chandra Joshi ,&nbsp;Somya Sinha ,&nbsp;Pooja Bhatnagar ,&nbsp;Yogesh Nath ,&nbsp;Bhavya Negi ,&nbsp;Vinod Kumar ,&nbsp;Prateek Gururani","doi":"10.1016/j.crmicr.2024.100237","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100237","url":null,"abstract":"<div><p>Due to an increase in industrialization and urbanization, massive amounts of solid waste biomass are speedily accumulating in our environment, which poses several adverse effects on habitat and human health thus becoming a matter of discussion in the environmental community. With reference to the circular economy, continuous efforts have been put forward for setting up an organised management approach in combination with an efficient treatment technique for increasing the profitable utilization of solid waste. This review aims to provide a systematic discussion on the recent thermochemical technologies employed for converting waste biomass generated from different sources into valuable products like biochar, bio-oil, heat, energy and syngas. The article further focuses on a few important aspects of thermochemical conversion of waste biomass to useful products like technical factors affecting thermochemical processes, applications of by-products of thermochemical conversion, and biological pretreatment of waste biomass. The review assists interesting recent and scientific trends for boosting up the systematic management and valorization of solid waste through low-cost, efficient, environment-friendly and sustainable technologies.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100237"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000191/pdfft?md5=dc9de733212561ec1f8aaa3281f44ba8&pid=1-s2.0-S2666517424000191-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extracellular vesicle production: A bidirectional effect in the interplay between host and Candida fungi 细胞外囊泡的产生:宿主与念珠菌相互作用的双向效应
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100255
Kamila Kulig, Maria Rapala-Kozik, Justyna Karkowska-Kuleta

Candida fungi exploit various virulence strategies to invade the human host, while host cells employ diverse mechanisms to maintain homeostasis and respond to infection. Extracellular vesicles (EVs) are integral components of the multifaceted landscape of host-pathogen interactions, with their abundant production by all contributors involved in these complex and dynamic relations. Herein, we present the current state of knowledge regarding the host response by releasing EVs in reaction to Candida, as well as the influence of fungal EVs on the functionality of the confronted host cells. Fungal vesicles contribute to enhanced adhesion of pathogens to human cells as evidenced for C. auris, and may modulate the production of several cytokines, including IL-6, IL-8, IL-10, IL-12p40, TGF-β and TNF-α, thereby exerting pro-infective and pro-inflammatory effects, as described for C. albicans and other Candida species. Whereas the biosynthesis of EVs by host cells can dynamically modulate the proliferation and viability of fungal cells and affect the candidacidal functionality of other effector cells. The reciprocal influence of EVs from host cells and Candida pathogens is a key focus, explaining their significant role in cell signaling and interkingdom communication.

念珠菌利用各种毒力策略入侵人类宿主,而宿主细胞则利用各种机制维持体内平衡和应对感染。细胞外囊泡(EVs)是宿主-病原体多方面相互作用的组成部分,参与这些复杂而动态关系的所有参与者都会大量产生EVs。在此,我们将介绍宿主对念珠菌释放 EVs 所做出的反应,以及真菌 EVs 对对抗宿主细胞功能的影响。真菌囊泡有助于增强病原体对人体细胞的粘附力,这一点在白色念珠菌中得到了证实,并可能调节多种细胞因子的产生,包括IL-6、IL-8、IL-10、IL-12p40、TGF-β和TNF-α,从而发挥促感染和促炎症的作用,这一点在白色念珠菌和其他念珠菌中也得到了证实。而宿主细胞生物合成的 EVs 可动态调节真菌细胞的增殖和活力,并影响其他效应细胞的念珠菌杀灭功能。宿主细胞和念珠菌病原体的 EVs 相互影响是研究的重点,这也解释了 EVs 在细胞信号传递和王国间交流中的重要作用。
{"title":"Extracellular vesicle production: A bidirectional effect in the interplay between host and Candida fungi","authors":"Kamila Kulig,&nbsp;Maria Rapala-Kozik,&nbsp;Justyna Karkowska-Kuleta","doi":"10.1016/j.crmicr.2024.100255","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100255","url":null,"abstract":"<div><p><em>Candida</em> fungi exploit various virulence strategies to invade the human host, while host cells employ diverse mechanisms to maintain homeostasis and respond to infection. Extracellular vesicles (EVs) are integral components of the multifaceted landscape of host-pathogen interactions, with their abundant production by all contributors involved in these complex and dynamic relations. Herein, we present the current state of knowledge regarding the host response by releasing EVs in reaction to <em>Candida</em>, as well as the influence of fungal EVs on the functionality of the confronted host cells. Fungal vesicles contribute to enhanced adhesion of pathogens to human cells as evidenced for <em>C. auris</em>, and may modulate the production of several cytokines, including IL-6, IL-8, IL-10, IL-12p40, TGF-β and TNF-α, thereby exerting pro-infective and pro-inflammatory effects, as described for <em>C. albicans</em> and other <em>Candida</em> species. Whereas the biosynthesis of EVs by host cells can dynamically modulate the proliferation and viability of fungal cells and affect the candidacidal functionality of other effector cells. The reciprocal influence of EVs from host cells and <em>Candida</em> pathogens is a key focus, explaining their significant role in cell signaling and interkingdom communication.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100255"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000373/pdfft?md5=d9cb8c5dc141ffcc023e09eb7274ba8d&pid=1-s2.0-S2666517424000373-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilizing novel Aspergillus species for bio-flocculation: A cost-effective approach to harvest Scenedesmus microalgae for biofuel production 利用新型曲霉菌进行生物絮凝:收获 Scenedesmus 微藻类用于生物燃料生产的经济有效方法
IF 4.8 Q1 MICROBIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100272
Gyanendra Tripathi , Vinay Kumar Pandey , Suhail Ahmad , Irum , Nortoji A. Khujamshukurov , Alvina Farooqui , Vishal Mishra

The present study aimed to isolate a bioflocculating fungal strain from wastewater collected from a local bike garage. The isolate showed maximum similarity to Aspergillus species. The fungus was identified as Aspergillus flavus species F_GTAF1 IU (accession no OP703382). The isolated fungus was evaluated in terms of biomass recovery efficiency in Scenedesmus Sp. GTAF01. The extent of algal fungal co-pelletization was evaluated as a function of the algae-to-fungi ratio, volume of fungal culture in broth, agitation rate, and pH. results showed that at fungal culture volume of 60%v/v, fungal culture volume of 1:3%w/w, 100 rpm, and pH 3, 93.6% biomass was obtained during the initial 5 h. At wavenumbers 1384 and 1024 cm-1 a significant alteration in the transmission percentage was observed in co-pellet compared to algae and fungal cells. This shows the significant role of C-H–H and C-N stretches in co-pellet formation. This study provides deep insight into effective microalgal harvesting along with the simultaneous extraction of lipids that can be used for the sustainable production of biodiesel.

本研究旨在从当地一家自行车修理厂收集的废水中分离出一种生物絮凝真菌菌株。分离出的菌株与曲霉菌的相似度最高。经鉴定,该真菌为黄曲霉 F_GTAF1 IU(登录号 OP703382)。对分离出的真菌进行了生物量恢复效率评估,结果显示,该真菌在 Scenedesmus Sp.GTAF01 的生物量恢复效率进行了评估。结果表明,当真菌培养量为 60░%v/v、真菌培养量为 1:3░%w/w、转速为 100 rpm、pH 值为 3 时,藻类与真菌的共造粒程度为 93.在 1384 和 1024 cm-1 波长处,与藻类和真菌细胞相比,共生颗粒的透射率发生了显著变化。这表明,C-H-H 和 C-N 伸展在共颗粒形成过程中发挥了重要作用。这项研究为有效收获微藻并同时提取脂质提供了深刻的见解,可用于生物柴油的可持续生产。
{"title":"Utilizing novel Aspergillus species for bio-flocculation: A cost-effective approach to harvest Scenedesmus microalgae for biofuel production","authors":"Gyanendra Tripathi ,&nbsp;Vinay Kumar Pandey ,&nbsp;Suhail Ahmad ,&nbsp;Irum ,&nbsp;Nortoji A. Khujamshukurov ,&nbsp;Alvina Farooqui ,&nbsp;Vishal Mishra","doi":"10.1016/j.crmicr.2024.100272","DOIUrl":"10.1016/j.crmicr.2024.100272","url":null,"abstract":"<div><p>The present study aimed to isolate a bioflocculating fungal strain from wastewater collected from a local bike garage. The isolate showed maximum similarity to <em>Aspergillus</em> species<em>.</em> The fungus was identified as <em>Aspergillus flavus</em> species <em>F_GTAF1 IU</em> (accession no OP703382). The isolated fungus was evaluated in terms of biomass recovery efficiency in <em>Scenedesmus</em> Sp. GTAF01. The extent of algal fungal co-pelletization was evaluated as a function of the algae-to-fungi ratio, volume of fungal culture in broth, agitation rate, and pH. results showed that at fungal culture volume of 60<span><math><mi>░</mi></math></span>%v/v, fungal culture volume of 1:3<span><math><mi>░</mi></math></span>%w/w, 100 rpm, and pH 3, 93.6<span><math><mi>░</mi></math></span>% biomass was obtained during the initial 5 h. At wavenumbers 1384 and 1024 cm<sup>-1</sup> a significant alteration in the transmission percentage was observed in co-pellet compared to algae and fungal cells. This shows the significant role of C-H–H and C-N stretches in co-pellet formation. This study provides deep insight into effective microalgal harvesting along with the simultaneous extraction of lipids that can be used for the sustainable production of biodiesel.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100272"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000543/pdfft?md5=b532aa94acec862a01da09a9be65b062&pid=1-s2.0-S2666517424000543-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Current Research in Microbial Sciences
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1