Current Research in Microbial Sciences最新文献_第7页

Rapid composting of groundnut residues through novel microbial consortium: Evaluating maturity, stability, and microbial activity 通过新型微生物群快速堆肥花生残渣：评估成熟度、稳定性和微生物活性

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100277

Deblina Roy , Sunil Kumar Gunri , Champak Kumar Kundu , Prasanta Kumar Bandyopadhyay

A laboratory pot experiment (Experiment 1) was conducted to determine the optimal ratio of groundnut haulm and shell as composting substrates. The aim was to identify the most effective combination for rapid decomposition under in vivo conditions. The experiment was carried out in 2022, from May to July, using a completely randomized design (CRD) with 6 treatments that were replicated 4 times. The treatment combinations in the pot experiment were as follows: T₁: haulm + shell (1:1), T₂: haulm + shell (2:1), T₃: haulm + shell (3:1), T₄: T₁ + C_n, T₅: T₂ + C_n, and T₆: T₃ + C_n. Here, C_n refers to the cellulose-degrading efficient microbial consortium containing bacterial strains Priestia megaterium DBJ6, Micrococcus yunnanensis DMB9, and fungal strains Aspergillus foetidus DAJ2, Trichoderma atrobrunnium DTJ4, and Phanerochaete chrysosporium CBS129.27. Based on the results (results of chemical and biological properties) of the pot experiment, treatment T₆ was considered the best treatment (the C/N ratio was 14.36 ± 0.444 after three months of decomposition) for further study under in vivo conditions. The in vivo experiment (Experiment 2) was conducted at the Jaguli Instructional Farm of Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India, during the months of August to October in both 2022 and 2023 with two treatments: the normal composting treatment (NC) and the rapid composting treatment (RC). The findings of Experiment 2 showed that organic substrates degraded more quickly (within 90 days) during rapid composting, as evidenced by a decrease in the C/N ratio to below 15 (14.32 and 13.97 on day 90 in 2022 and 2023, respectively). Whereas, normal composting required more than 90 days to achieve a C/N ratio below 20 (23.80 and 23.15 on day 90 in 2022 and 2023, respectively). The RC treatment also showed a higher microbial population and enzyme activity compared to the NC treatment. Therefore, according to the results of this experiment, it can be concluded that the inoculation of the microbial consortium was more beneficial for accelerating the composting process under the same composting circumstances and substrate ratio.

为了确定花生秆和壳作为堆肥基质的最佳比例，我们进行了一次实验室盆栽实验（实验 1）。目的是确定在活体条件下快速分解的最有效组合。实验于 2022 年 5 月至 7 月进行，采用完全随机设计（CRD），6 个处理重复 4 次。盆栽实验中的处理组合如下：T1：秆+壳（1:1）；T2：秆+壳（2:1）；T3：秆+壳（3:1）；T4：T1：T1 + Cn；T5：T2 + Cn；T6：T3 + Cn。这里的 Cn 指的是纤维素降解高效微生物联合体，包括细菌菌株 Priestia megaterium DBJ6、Micrococcus yunnanensis DMB9 和真菌菌株 Aspergillus foetidus DAJ2、Trichoderma atrobrunnium DTJ4 和 Phanerochaete chrysosporium CBS129.27。根据盆栽实验的结果（化学和生物特性结果），处理 T6 被认为是最佳处理（三个月分解后的 C/N 比为 14.36 ± 0.444），可在体内条件下进一步研究。活体实验（实验 2）于 2022 年和 2023 年的 8 月至 10 月在印度西孟加拉邦 Bidhan Chandra Krishi Viswavidyalaya 的 Jaguli 教学农场进行，分为两种处理：普通堆肥处理（NC）和快速堆肥处理（RC）。实验 2 的结果表明，在快速堆肥过程中，有机基质的降解速度更快（90 天内），具体表现为 C/N 比值降至 15 以下（2022 年和 2023 年第 90 天分别为 14.32 和 13.97）。而正常堆肥需要 90 多天才能使 C/N 比值低于 20（2022 年和 2023 年第 90 天分别为 23.80 和 23.15）。与 NC 处理相比，RC 处理的微生物数量和酶活性也更高。因此，根据本实验的结果，可以得出结论：在相同的堆肥环境和基质比例下，接种微生物菌群更有利于加速堆肥过程。

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引用次数: 0

Simultaneous production of biofuel from agricultural wastes and bioremediation of the waste substrates: A review 同时利用农业废弃物生产生物燃料和对废弃物基质进行生物修复：综述

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100305

Chukwuemeka Samson Ahamefule , Chidimma Osilo , Blessing C. Ahamefule , Stella N. Madueke , Anene N. Moneke

Pollution from fossil fuel usage coupled with its unsustainability is currently instigating a global drive for affordable and eco-friendly alternatives. A feasible replacement seems to be microbial biofuels. However, the production cost is still high, partly due to the cost of substrates and media. Microalgae, yeasts, moulds and bacteria can grow on cheap and easily available agricultural waste substrates to produce bioethanol, biogas, biobutanol, acetone and/or lipids for biodiesel. Oleaginous microbes, such as several species of Chlorella, Schizochytrium, Rhodotorula, Rhodosporidium, Yarrowia, Aspergillus, Rhodococcus, etc., have been applied in the production of high and choice lipids for biodiesel. High bioethanol, butanol and acetone yields have also been achieved with several agricultural waste substrates either through separate hydrolysis and fermentation or simultaneous saccharification and fermentation. The isolation or transformation of some microbial strains has shown the possibility of using only one microorganism to produce bioethanol from lignocellulose biomass in consolidated bioethanol production. High biogas/biomethane yield has also been recorded from different agricultural wastes, especially in co-digestion systems. Pre-treatment, detoxification, application of microbial co-cultures, co-substrate mixing ratios, organic loading rate, hydraulic retention time among others have been reported to affect the quantity and quality of different biofuels produced from agricultural wastes. Interestingly, these agricultural waste substrates were greatly degraded during fermentation and/or digestion to achieve high level of bioremediation. Therefore, this study presents a holistic review of various agricultural wastes adopted as biofuel substrates, the conditions to attain optimum biofuel productions and cases of simultaneous bioremediation of substrates obtained during biofuel production. The valorization of the biofuel by-products into other essential products to achieve a zero-waste and circular bioeconomy is also properly presented.

化石燃料的使用所造成的污染及其不可持续性目前正在全球范围内推动寻找经济实惠、生态友好的替代品。微生物生物燃料似乎是一种可行的替代品。然而，生产成本仍然很高，部分原因是基质和培养基的成本。微藻类、酵母菌、霉菌和细菌可以在廉价且容易获得的农业废弃物基质上生长，生产生物乙醇、沼气、生物丁醇、丙酮和/或用于生物柴油的脂质。发油微生物，如小球藻、裂殖藻、红球藻、红孢子菌、Yarrowia、曲霉、红球菌等，已被用于生产生物柴油所需的高脂和精选脂质。通过单独水解和发酵或同时糖化和发酵，几种农业废弃物基质也获得了较高的生物乙醇、丁醇和丙酮产量。一些微生物菌株的分离或转化表明，在综合生物乙醇生产中，只用一种微生物就能从木质纤维素生物质中生产生物乙醇。从不同的农业废弃物中，特别是在共消化系统中，也记录到了较高的沼气/生物甲烷产量。据报道，预处理、解毒、微生物共培养物的应用、共基质混合比、有机物负载率、水力停留时间等因素都会影响农业废弃物生产不同生物燃料的数量和质量。有趣的是，这些农业废弃物基质在发酵和/或消化过程中被大量降解，以实现高水平的生物修复。因此，本研究全面回顾了用作生物燃料基质的各种农业废弃物、实现最佳生物燃料生产的条件以及在生物燃料生产过程中同时对基质进行生物修复的案例。此外，还适当介绍了如何将生物燃料副产品转化为其他重要产品，以实现零废物和循环生物经济。

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引用次数: 0

Four recent insights suggest the need for more refined methods to assess the resistogenicity of doxycycline post exposure prophylaxis 最近的四项研究表明，需要采用更精细的方法来评估多西环素暴露后预防的抗原性

Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100234

Thibaut Vanbaelen , Sheeba Santhini Manoharan-Basil , Chris Kenyon

Two recently published randomized trials of doxycycline post exposure prophylaxis (PEP) have concluded that this intervention is highly effective at reducing the incidence of bacterial sexually transmitted infections (STIs) and has little or no risk of promoting the spread of antimicrobial resistance (AMR). In this perspective piece, we review four types of evidence that suggest that the risk of promoting AMR has been inadequately assessed in these studies. 1) The studies have all used proportion resistant as the outcome measure. This is a less sensitive measure of resistogenicity than MIC distribution. 2) These RCTs have not considered population-level pathways of AMR selection. 3) In populations with very high antimicrobial consumption such as PrEP cohorts, the relationship between antimicrobial consumption and resistance may be saturated. 4) Genetic linkage of AMR means that increased tetracycline use may select for AMR to not only tetracyclines but also other antimicrobials in STIs and other bacterial species. We recommend novel study designs to more adequately assess the AMR-inducing risk of doxycycline PEP.

最近发表的两项关于强力霉素暴露后预防疗法（PEP）的随机试验得出结论：这种干预措施在降低细菌性性传播感染（STI）发病率方面非常有效，而且几乎没有促进抗菌药耐药性（AMR）传播的风险。在这篇透视文章中，我们回顾了四类证据，这些证据表明这些研究对促进 AMR 的风险评估不足。1) 这些研究都使用耐药比例作为结果衡量标准。与 MIC 分布相比，这一指标对抗药性的敏感性较低。2) 这些 RCT 研究没有考虑 AMR 在人群中的选择途径。3) 在抗菌药物消耗量非常高的人群（如 PrEP 队列）中，抗菌药物消耗量与耐药性之间的关系可能已经饱和。4）AMR 的遗传联系意味着四环素使用量的增加可能不仅会导致对四环素的 AMR，还会导致对性传播感染和其他细菌物种中其他抗菌药物的 AMR。我们建议采用新的研究设计，以更充分地评估强力霉素 PEP 诱导 AMR 的风险。

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引用次数: 0

Revealing the impact of Rapamycin on the virulence factors of the Candida haemulonii complex 揭示雷帕霉素对血念珠菌复合体毒力因子的影响

Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100247

Vinicius Alves , Iara Bastos de Andrade , Dario Corrêa-Junior , Igor Avellar-Moura , Karini Passos , Juliana Soares , Bruno Pontes , Marcos Abreu Almeida , Rodrigo Almeida-Paes , Susana Frases

The incidence of invasive fungal infections caused by Candida species is increasing, particularly in immunocompromised individuals. This increasing incidence poses a dual challenge, comprising escalating antifungal resistance and the necessity for accurate fungal identification. The Candida haemulonii complex further complicates these challenges due to limited identification tools. Like some other Candida species, infections involving this complex show resistance to multiple antifungals, requiring innovative therapeutic approaches. Rapamycin, known for its antifungal properties and immunosuppressive characteristics, was investigated against the C. haemulonii complex species. Results revealed a rapamycin minimal inhibitory concentration (MIC) range of 0.07 to >20 µM, with fungicidal effects in most strains. In vitro analyses using the rapamycin maximum plasma concentration (0.016 µM) showed reduced surface properties and decreased production of extracellular enzymes. Rapamycin also hindered biofilm formation by some strains. Even when treated at the human therapeutic dose, which is lower than the MIC, phenotypic variations in C. haemulonii were detected, hinting at the possible attenuation of some virulence factors when exposed to rapamycin.

由念珠菌引起的侵袭性真菌感染发病率正在上升，尤其是在免疫力低下的人群中。发病率的上升带来了双重挑战，包括抗真菌耐药性的升级和准确鉴定真菌的必要性。由于鉴定工具有限，血液念珠菌复合体使这些挑战变得更加复杂。与其他一些白色念珠菌一样，该复合菌群的感染表现出对多种抗真菌药物的耐药性，需要创新的治疗方法。雷帕霉素以其抗真菌特性和免疫抑制特性而闻名，我们研究了雷帕霉素对血念珠菌复合菌种的作用。结果显示，雷帕霉素的最小抑菌浓度（MIC）范围为 0.07 至 20 µM，对大多数菌株都有杀真菌作用。使用雷帕霉素最大血浆浓度（0.016 µM）进行的体外分析表明，雷帕霉素降低了表面特性，减少了细胞外酶的产生。雷帕霉素还能阻止某些菌株形成生物膜。即使以低于 MIC 的人体治疗剂量进行治疗，也能检测到血球孢子菌的表型变化，这表明雷帕霉素可能会削弱某些毒力因子。

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引用次数: 0

Advances in microbial based bio-inoculum for amelioration of soil health and sustainable crop production 基于微生物的生物接种物在改善土壤健康和可持续作物生产方面的进展

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100251

Aurodeepa Samantaray , Sourav Chattaraj , Debasis Mitra , Arindam Ganguly , Rahul Kumar , Ashish Gaur , Pradeep K.Das Mohapatra , Sergio de los Santos-Villalobos , Anju Rani , Hrudayanath Thatoi

The adoption of sustainable agricultural practices is increasingly imperative in addressing global food security and environmental concerns, with microbial based bio-inoculums emerging as a promising approach for nurturing soil health and fostering sustainable crop production.This review article explores the potential of microbial based bio-inoculumsor biofertilizers as a transformative approach toenhance plant disease resistance and growth. It explores the commercial prospects of biofertilizers, highlighting their role in addressing environmental concerns associated with conventional fertilizers while meeting the growing demand for eco-friendly agricultural practices. Additionally, this review discusses the future prospects of biofertilizers, emphasizing the ongoing advancements in biotechnology and formulation techniques that are expected to enhance their efficacy and applicability. Furthermore, this article provides insights into strategies for the successful acceptance of biofertilizers among farmers, including the importance of quality control, assurance, and education initiatives to raise awareness about their benefits and overcome barriers to adoption. By synthesizing the current research findings and industrial developments, this review offers valuable guidance for stakeholders seeking to exploit the potential of biofertilizers or beneficial microbes to promote soil health, ensure sustainable crop production, and addressing the challenges of modern agriculture.

在解决全球粮食安全和环境问题方面，采用可持续农业实践日益重要，而基于微生物的生物接种物正在成为培育土壤健康和促进可持续作物生产的一种有前途的方法。这篇综述文章探讨了基于微生物的生物接种物或生物肥料作为提高植物抗病性和生长的一种变革性方法的潜力。文章探讨了生物肥料的商业前景，强调了生物肥料在解决与传统肥料相关的环境问题方面的作用，同时满足了人们对生态友好型农业实践日益增长的需求。此外，这篇综述还讨论了生物肥料的未来前景，强调生物技术和配方技术的不断进步有望提高生物肥料的功效和适用性。此外，本文还深入探讨了农民成功接受生物肥料的策略，包括质量控制、保证和教育措施的重要性，以提高对生物肥料益处的认识，克服采用生物肥料的障碍。本综述综合了当前的研究成果和工业发展，为利益相关者提供了宝贵的指导，帮助他们挖掘生物肥料或有益微生物的潜力，促进土壤健康，确保作物可持续生产，应对现代农业的挑战。

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引用次数: 0

Isolation and identification of Rhizospheric and Endophytic Bacteria from Cucumber plants irrigated with wastewater: Exploring their roles in plant growth promotion and disease suppression 从用废水灌溉的黄瓜植物中分离和鉴定根瘤菌和内生菌：探索它们在促进植物生长和抑制病害方面的作用

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100256

Kumar Shreshtha , Satyam Raj , Arun Kumar Pal , Pooja Tripathi , Krishna Kumar Choudhary , Debasis Mitra , Anju Rani , Sergio de los Santos-Villalobos , Vijay Tripathi

Wastewater contains various emerging contaminants, including heavy metals, residues of pesticides, and pharmaceuticals. Therefore, irrigation with wastewater can enhance heavy metal contamination in soil and adversely affect plant growth. To mitigate this problem, plant growth-promoting bacteria (PGPR) can improve plant growth under heavy metal stress. This study aimed to isolate and characterize rhizospheric and endophytic bacteria from the rhizosphere soil and roots of a cucumber plant irrigated with municipal wastewater. A total of 121 morphologically distinct bacterial isolates from the rhizosphere and 90 bacterial isolates from the endophytic region were isolated and tested for heavy metal resistance and in vitro plant growth-promoting characteristics, including indole-3-acetic acid (IAA) production, phosphate solubilization, Hydrogen Cyanide (HCN) production, and siderophore production. Most of the bacteria analyzed from the rhizospheric and endophytic regions showed various plant growth-promoting characteristics and were tolerant to different heavy metals at various concentrations. Bacterial strains R1 (Proteus sp.) and E2 (Bacillus sp.) were antagonistic to Fusarium oxysporum f. sp. Lycopersici. Wastewater irrigation increases heavy metal-resistant bacteria in cucumber plants, which can alleviate heavy metal stress. Additionally, Proteus sp. and Bacillus sp. isolates are potential candidates for removing heavy metal-contaminated soil and could be potential biofertilizer candidates for selected plants and biocontrol agents.

废水中含有各种新出现的污染物，包括重金属、残留农药和药物。因此，用废水灌溉会加重土壤中的重金属污染，对植物生长造成不利影响。为缓解这一问题，植物生长促进菌（PGPR）可改善重金属胁迫下的植物生长状况。本研究旨在从使用城市污水灌溉的黄瓜植株的根圈土壤和根部分离出根瘤菌和内生菌，并确定其特征。研究人员从根瘤菌层分离出 121 个形态各异的细菌分离株，从内生菌区分离出 90 个细菌分离株，并对它们的重金属抗性和体外植物生长促进特性（包括吲哚-3-乙酸（IAA）产生、磷酸盐溶解、氰化氢（HCN）产生和苷元产生）进行了检测。从根瘤区和内生区分析的大多数细菌都表现出各种促进植物生长的特性，并能耐受不同浓度的重金属。细菌菌株 R1（变形杆菌）和 E2（芽孢杆菌）对 Fusarium oxysporum f. sp. Lycopersici 具有拮抗作用。废水灌溉可增加黄瓜植株中的重金属抗性细菌，从而缓解重金属胁迫。此外，变形杆菌和芽孢杆菌分离物是清除重金属污染土壤的潜在候选菌种，也可作为选定植物的潜在生物肥料和生物控制剂。

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引用次数: 0

Agro-industrial waste to microbial sophorolipids: Innovations and impacts on the food industry 从农用工业废物到微生物槐脂：创新及其对食品工业的影响

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100275

Aishwary Purohit , Amar Jyoti Das , Debashish Ghosh

The cost-effective production of sophorolipids (SLs) using agro-industrial waste represents a significant advancement in sustainable practices within the food industry. Sophorolipids, known for their excellent emulsifying and antimicrobial properties, offer a promising natural alternative to synthetic preservatives, which can pose health and environmental risks. This study aims to critically assess the strategies for producing sophorolipids from agro-industrial waste, with a focus on their implications for improving food safety and quality. By integrating techno-economic analysis (TEA) and life cycle assessment (LCA), the review provides a comprehensive evaluation of the effectiveness, feasibility, and sustainability of these methods. The scientific importance of this research lies in its potential to enhance waste management practices and promote eco-friendly food preservation solutions, contributing to the development of safer and more sustainable industrial processes.

利用农用工业废料生产具有成本效益的槐脂（SLs）是食品工业可持续发展实践中的一大进步。槐脂以其出色的乳化和抗菌特性而闻名，是一种很有前途的天然防腐剂替代品，而合成防腐剂会对健康和环境造成危害。本研究旨在批判性地评估利用农用工业废物生产槐脂的策略，重点关注其对提高食品安全和质量的影响。通过整合技术经济分析（TEA）和生命周期评估（LCA），该综述对这些方法的有效性、可行性和可持续性进行了全面评估。这项研究的科学意义在于，它有可能加强废物管理实践，推广生态友好型食品保鲜解决方案，促进更安全、更可持续的工业流程的发展。

引用次数: 0

Corrigendum to “Unravelling the diversity of Anaplasma species circulating in selected African wildlife hosts by targeted 16S microbiome analysis” [Current Research in Microbial Sciences Volume 5 (2023) 100198] 对 "通过有针对性的 16S 微生物组分析揭示在选定的非洲野生动物宿主中循环的阿那普拉斯菌物种的多样性"[《微生物科学研究》第 5 卷（2023 年）第 100198 期]的更正

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100279

S. Marcus Makgabo , Kelly A. Brayton , Marinda C. Oosthuizen , Nicola E. Collins

引用次数: 0

Alpaca-derived nanobody targeting the hydrophobic pocket of the HIV-1 gp41 NHR broadly neutralizes HIV-1 by blocking six-helix bundle formation 针对 HIV-1 gp41 NHR 疏水袋的羊驼源性纳米抗体通过阻断六螺旋束的形成广泛中和 HIV-1

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100263

Lujia Sun , Bo Chen , Xianbo Liu , Yun Zhu , Guangxu Zhang , Xiaoxing Liang , Lixiao Xing , Wei Xu , Shibo Jiang , Xinling Wang

The highly conserved hydrophobic pocket region of HIV-1 gp41 NHR triple-stranded coiled coil is crucial for the binding of CHR to NHR to form a six-helix bundle (6-HB). This pocket is only exposed instantaneously during fusion, making it an ideal target for antibody drug design. However, IgG molecule is too big to enter the pocket during the fusion process. Therefore, to overcome the steric hindrance and kinetic obstacles caused by the formation of gp41 pre-hairpin fusion intermediate, we obtained nanobodies (Nbs) targeting NHR by immunizing alpaca with an NHR-trimer mimic. Specifically, we identified a Nb, Nb-172, that exhibited potent and broadly neutralizing activity against HIV-1 pseudoviruses, HIV-1 primary isolates, and T20-resistant HIV-1 strains. In addition, the combinatorial use of mD1.22 and Nb-172 exhibited synergism in inhibiting HIV-1 infection inactivating cell-free virions. Nb-172 can competitively bind to the hydrophobic pocket of gp41 NHR to inhibit 6-HB formation. These findings suggest that Nb-172 merits further investigation as a potential therapeutic for HIV-1 infection.

HIV-1 gp41 NHR 三股线圈中高度保守的疏水口袋区域对于 CHR 与 NHR 结合形成六螺旋束（6-HB）至关重要。这个口袋只在融合过程中瞬间暴露，因此是抗体药物设计的理想靶点。然而，IgG 分子太大，无法在融合过程中进入口袋。因此，为了克服gp41前发夹融合中间体形成时产生的立体阻碍和动力学障碍，我们用NHR三聚体模拟物免疫羊驼，获得了靶向NHR的纳米抗体（Nbs）。具体来说，我们发现了一种 Nb（Nb-172），它对 HIV-1 伪病毒、HIV-1 原始分离株和 T20 抗性 HIV-1 株具有强效和广泛的中和活性。此外，mD1.22 和 Nb-172 的组合使用在抑制 HIV-1 感染灭活无细胞病毒方面表现出协同作用。Nb-172 可与 gp41 NHR 的疏水口袋竞争性结合，抑制 6-HB 的形成。这些研究结果表明，Nb-172 作为一种治疗 HIV-1 感染的潜在药物值得进一步研究。

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引用次数: 0

Paddy seeds bacterization with ACC deaminase producing endophyte Alcaligenes faecalis SSP8 regulates physiology, leaves gas exchange parameters, PSII photochemistry and antioxidant enzymes metabolism in NaCl stressed seedlings 水稻种子加入产生ACC脱氨酶的内生菌Alcaligenes faecalis SSP8可调节NaCl胁迫秧苗的生理机能、叶片气体交换参数、PSII光化学和抗氧化酶代谢

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2024-01-01 DOI: 10.1016/j.crmicr.2024.100299

Shobhit Raj Vimal , Jay Shankar Singh , Sheo Mohan Prasad

The endophytic microbes play crucial roles to crop development under stress environmental conditions. In this research, 36 endophytic bacterial strains having diverse morphology were isolated from exotic wild plant Croton bonplandianus. The strain SSP8 was selected for experimental study as it efficiently tolerate NaCl (0–1200 mM), produced Indole-3-acetic acid (IAA) (46 μg mL^-1) and 1-amino-1-cyclopropane-1-carboxylate (ACC) deaminase (176.70 nmol α-ketobutyrate mg^-1 protein h^-1). The SSP8 was identified as Alcaligenes faecalis with 16 S r-RNA gene sequencing and submitted to NCBI-USA with accession number OR225818. The A. faecalis SSP8 significantly enhanced paddy seeds germination percentage, seedlings vigour index and vegetative growth parameters under different NaCl (0–180 mM) regimes. The paddy seedlings chlorophyll contents, Chl-a fluorescence transient (PSII photochemistry), leaves gas exchange parameters were significantly enhanced in A. faecalis SSP8+NaCl (0–180 mM) conditions. The oxidative stress biomarkers and antioxidant enzymes activities were significantly declined in A. faecalis SSP8+NaCl (0–180 mM) treated seedlings. In conclusion, based on the above results the paddy seeds bacterization with A. faecalis SSP8 could be a bio-prospective tool to alleviate the NaCl stress and enhance the paddy crop agriculture productivity in salt affected marginal soils.

在压力环境条件下，内生微生物对作物生长起着至关重要的作用。本研究从外来野生植物 Croton bonplandianus 中分离出 36 株形态各异的内生细菌。由于菌株 SSP8 能有效耐受 NaCl（0-1200 mM），产生吲哚-3-乙酸（IAA）（46 μg mL-1）和 1-氨基-1-环丙烷-1-羧酸（ACC）脱氨酶（176.70 nmol α-ketobutyrate mg-1 protein h-1），因此被选中进行实验研究。通过 16 S r-RNA 基因测序，确定该 SSP8 为粪链球菌，并提交给 NCBI-USA，登录号为 OR225818。在不同的 NaCl（0-180 mM）条件下，粪酵母菌 SSP8 能显著提高水稻种子的发芽率、幼苗活力指数和无性生长参数。在 A. faecalis SSP8+NaCl (0-180 mM) 条件下，水稻幼苗的叶绿素含量、Chl-a 荧光瞬时（PSII 光化学）、叶片气体交换参数均有明显提高。在粪酵母菌 SSP8+NaCl （0-180 mM）处理的幼苗中，氧化应激生物标志物和抗氧化酶活性明显下降。总之，根据上述结果，用粪杆菌 SSP8 对水稻种子进行细菌培养可以作为一种生物前瞻性工具，缓解受盐影响的边缘土壤中的 NaCl 胁迫，提高水稻作物的农业生产力。

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引用次数: 0