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Enhancing bacterial inhibition in beetroot beverages: The potent combination of sappan heartwood extracts via accelerated solvent extraction and pulsed electric field methods 增强甜菜根饮料中的细菌抑制能力:通过加速溶剂萃取和脉冲电场方法将苏枋心材提取物有效结合起来
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-27 DOI: 10.1016/j.jafr.2024.101268
Rattana Muangrat , Yaowares Thipsuwan

This study aimed to investigate the potential of sappan heartwood extract as an inhibitor of foodborne pathogenic bacteria in beetroot juice. The extract was obtained using an accelerated solvent extraction process (using an ethanol/water solvent mixture (3:1 w/w) at 120 °C and 1500 psi for 5 min), yielding 10.04 g per 73.6 g of dried sappan heartwood powder, with a percentage yield of 13.64 and 2.69 % brazilin. The extract was combined with pulsed electric fields (PEF) at different strengths and tested against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus), and Salmonella typhimurium (S. typhimurium). Results showed that the combination of sappan heartwood extract and PEF at 15 kV/cm and concentrations of 3.91, 5.86, and 11.73 mg/mL effectively reduced the total plate count and coliform bacteria count, and completely eliminated B. cereus and S. typhimurium during 7 days of storage at 4 °C. Furthermore, this combination was found to be more efficient in bacterial elimination compared to thermal pasteurization and potassium sorbate addition. These findings suggest that sappan heartwood extract can be used as a natural antimicrobial agent in food products, especially in beetroot juice, where regulatory standards restrict the addition of preservatives. Using sappan heartwood extract in combination with PEF is highly effective in killing foodborne pathogens, reducing the risk of consumer harm and ensuring food safety.

本研究旨在探讨苏木心材提取物作为甜菜根汁中食源性致病菌抑制剂的潜力。该提取物采用加速溶剂萃取工艺(使用乙醇/水溶剂混合物(3:1 w/w),在 120 °C 和 1500 psi 下萃取 5 分钟)获得,每 73.6 克干燥苏木心材粉末可提取 10.04 克,提取率为 13.64%,巴西苷含量为 2.69%。该提取物与不同强度的脉冲电场(PEF)相结合,对大肠杆菌(E. coli)、金黄色葡萄球菌(S. aureus)、蜡样芽孢杆菌(B. cereus)和鼠伤寒沙门氏菌(S. typhimurium)进行了测试。结果表明,在 15 千伏/厘米、浓度分别为 3.91、5.86 和 11.73 毫克/毫升的条件下,将苏木心材提取物与 PEF 结合使用,可有效减少菌落总数和大肠菌群数量,并在 4 ℃ 下储存 7 天期间完全消除蜡样芽孢杆菌和伤寒沙门氏菌。此外,与热巴氏杀菌法和添加山梨酸钾法相比,这种组合的除菌效果更好。这些研究结果表明,苏木心材提取物可用作食品中的天然抗菌剂,特别是在甜菜根汁中,因为监管标准限制添加防腐剂。将苏木心材提取物与 PEF 结合使用,可高效杀灭食源性病原体,降低对消费者造成伤害的风险,确保食品安全。
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引用次数: 0
Improved textural properties of transglutaminase treated milk acid gel: Influence of citric acid 改善转谷氨酰胺酶处理乳酸凝胶的质构特性:柠檬酸的影响
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-27 DOI: 10.1016/j.jafr.2024.101270
Jing Wang , Weibo Zhang , Qianwan Guo , Wanzhu Li , Yang Hong , Jing Gong , Feng Zhang , Chong Chen

Keeping the stability of yogurt during the fermentation, storage and transportation process is necessary to reduce the syneresis. To achieve this goal, the combination of the acid treatment and transglutaminase (TG) was proposed to improve the textural properties of milk acid gels. We investigated the synergistic effect of acid treatment and TG on the water holding capacity (WHC) and physical stability of milk acid gels. As the pH of the skimmed milk decreased from 6.7 to 5.2, the casein micelles dissociated into smaller particles, exposing more site for the crosslinking of TG. The WHC and stability of the TG-treated acid gels were significantly enhanced as the particle size of casein micelles decreased. Our results suggested that the combination of the treatment of acid and TG could significantly improve the textural properties of milk acid gels by the dissociation of casein micelles induced and increase in the covalent-crosslinking. This study provided a simple and novel method to improve the stability of the milk gels, which showed enormous potential for dairy processing.

在发酵、贮藏和运输过程中保持酸奶的稳定性对于减少酸奶凝固现象十分必要。为了实现这一目标,有人提出将酸处理和转谷氨酰胺酶(TG)结合起来,以改善乳酸凝胶的质构特性。我们研究了酸处理和转谷氨酰胺酶对乳酸凝胶持水量(WHC)和物理稳定性的协同作用。当脱脂乳的 pH 值从 6.7 降到 5.2 时,酪蛋白胶束解离成更小的颗粒,从而为 TG 的交联暴露出更多的位点。随着酪蛋白胶束粒径的减小,TG处理过的酸凝胶的WHC和稳定性也显著提高。我们的研究结果表明,酸和 TG 的联合处理可通过酪蛋白胶束的解离和共价交联的增加显著改善牛奶酸凝胶的质构特性。这项研究为提高牛奶凝胶的稳定性提供了一种简单而新颖的方法,为乳制品加工提供了巨大的潜力。
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引用次数: 0
Experimental and life cycle assessments of tomato (Solanum lycopersicum) cultivation under controlled environment agriculture 受控环境农业下番茄(Solanum lycopersicum)种植的实验和生命周期评估
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-27 DOI: 10.1016/j.jafr.2024.101266
Farhat Abbas , Awni Al-Otoom , Salem Al-Naemi , Azad Ashraf , Hassan Mahasneh

Crop production system assessments can evaluate if controlled environment agriculture is feasible as compared with semi-controlled and/or uncontrolled conditions. This study assessed the growth of tomatoes (Solanum lycopersicum) in three different environmental conditions (a fully controlled greenhouse mimicking controlled environment agriculture, a semi-controlled nethouse, and an uncontrolled open space) and three nutrient applications including nutrients supplied with i) 100 % inorganic fertilizer, ii) 100 % compost, and a mixture of 50 % of i and ii to be termed as mixed fertilizer from here onward. A life cycle assessment of tomato production in the greenhouse, nethouse, and open space cultivation phases was carried out using experimental data. A life cycle directory, a holistic cradle-to-gate concept, OpenLCA software, and commercial databases were used to evaluate the experimental activities’ potential for acidification, eutrophication, global warming, human toxicity, ozone depletion, and photochemical ozone creation. The environmental conditions and nutrient applications had individual and interactive effects on plant growth variables, fruit yield, and water use efficiency. For example, under the individual effects, the fruit yield means of greenhouse (9.52 ± 2.88 Mg/ha), nethouse (14.4 ± 3.64 Mg/ha), and open space (10.9 ± 1.88 Mg/ha) were significantly (P ≤ 0.05) different from one another. Fruit yield for the mixed fertilizer treatment (14.7 ± 3.06 Mg/ha) was 22.3 % and 41.9 % higher than those of inorganic fertilizer (11.5 ± 1.86 Mg/ha) and compost (8.55 ± 2.15 Mg/ha) treatments alone. The life cycle analysis showed that the phases of the nursery and product transportation (in all 3 cultivation phases) and irrigation systems in the nethouse and open space phases were the hotspots for their environmental impact. Replacement of irrigation pumps run on government electricity is suggested with solar-powered ones for nethouse and open space. Despite lower tomato yield, a fully controlled greenhouse was considered environmentally safe and the most efficient controlled environment agriculture system (with a proper supplemental lighting system) as it can operate year-round to produce an optimum annual yield of horticultural crops in arid and humid environments of the Arabian Peninsula. The significance of this study includes i) the production of Qatar-specific experimental data that is scarce in the literature, ii) the findings that insufficient supplemental lights could lower tomato yield from a greenhouse compared to a nethouse, iii) the life cycle-based environmental burdens of crop production systems, and iv) discoveries that, for arid regions, the nethouse cultivation could serve as economically viable, energy-efficient and environment-friendly tomato production systems.

作物生产系统评估可以评估受控环境农业与半受控和/或不受控条件相比是否可行。本研究评估了番茄(Solanum lycopersicum)在三种不同环境条件(模拟受控环境农业的完全受控温室、半受控网室和不受控制的空地)和三种养分施用条件下的生长情况,包括 i) 100% 无机肥料、ii) 100% 堆肥以及 i 和 ii 各占 50% 的混合肥料。利用实验数据对温室、网室和露地栽培阶段的番茄生产进行了生命周期评估。利用生命周期目录、"从摇篮到餐桌 "的整体概念、OpenLCA 软件和商业数据库,评估了实验活动对酸化、富营养化、全球变暖、人类毒性、臭氧消耗和光化学臭氧生成的潜在影响。环境条件和养分施用对植物生长变量、果实产量和水分利用效率有单独和交互影响。例如,在个体效应下,温室(9.52 ± 2.88 兆克/公顷)、网棚(14.4 ± 3.64 兆克/公顷)和露地(10.9 ± 1.88 兆克/公顷)的果实产量均值差异显著(P ≤ 0.05)。混合肥料处理的果实产量(14.7 ± 3.06 兆克/公顷)比单独施用无机肥(11.5 ± 1.86 兆克/公顷)和堆肥(8.55 ± 2.15 兆克/公顷)的果实产量分别高出 22.3% 和 41.9%。生命周期分析表明,育苗和产品运输阶段(所有 3 个栽培阶段)以及网室和空地阶段的灌溉系统是其环境影响的热点。建议在温室和露地栽培中使用太阳能灌溉系统取代政府供电的灌溉水泵。尽管番茄产量较低,但完全受控的温室被认为是环境安全和最有效的受控环境农业系统(有适当的补充照明系统),因为它可以全年运作,在阿拉伯半岛干旱潮湿的环境中生产最佳年产量的园艺作物。这项研究的意义包括:i) 编制了文献中很少见的卡塔尔特定实验数据;ii) 发现与网室相比,补充照明不足会降低温室的番茄产量;iii) 基于作物生产系统生命周期的环境负担;iv) 发现在干旱地区,网室栽培可作为经济上可行、节能和环保的番茄生产系统。
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引用次数: 0
Pursuing circular economics through the integrated crop-livestock systems: An integrative review on practices, strategies and challenges post Green Revolution in Indonesia 通过作物-牲畜综合系统追求循环经济:印度尼西亚绿色革命后的实践、战略和挑战综述
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-27 DOI: 10.1016/j.jafr.2024.101269
Dewa Ketut Sadra Swastika , Atien Priyanti , Abdul Muis Hasibuan , Dewi Sahara , Nyoman Ngurah Arya , Afrizal Malik , Nyak Ilham , Apri Laila Sayekti , Joko Triastono , Robet Asnawi , Dedi Sugandi , Nur Qomariah Hayati , Atman Atman

The Green Revolution, driven by advanced technology, substantially increased food production in Asian developing countries, including Indonesia. Nevertheless, it also resulted in environmental consequences, notably soil fertility degradation, threatening future agricultural productivity. As an alternative approach, the Integrated Crops-Livestock System (ICLS) holds promise for simultaneously enhancing crop and livestock yields while upholding environmental sustainability. This article investigates the significance of ICLS as a sustainable agricultural solution in the context of circular economics post Green Revolution era in Indonesia. This study employs an integrative literature review, encompassing secondary data analysis, research findings, and publications, to underscore the potential of ICLS in Indonesia and beyond. Despite its potential, the limited adoption of ICLS among Indonesian farmers is attributed to factors such as inadequate knowledge and skills, insufficient policy support, and inadequate dissemination and promotion efforts. To promote widespread ICLS implementation, robust dissemination strategies are imperative, encompassing technical guidance, counseling, training, and on-farm demonstrations. In addition, policy support, including accessible credit and price incentives, is essential to incentivize farmers to embrace ICLS practices. This article underscores the relevance of circular economics within the context of integrated farming and provides insights for policymakers and stakeholders aiming to promote sustainable agriculture in Indonesia's post Green Revolution era.

在先进技术的推动下,绿色革命大大提高了包括印度尼西亚在内的亚洲发展中国家的粮食产量。然而,它也造成了环境后果,特别是土壤肥力退化,威胁到未来的农业生产力。作为一种替代方法,作物-牲畜综合系统(ICLS)有望在提高作物和牲畜产量的同时维护环境的可持续发展。本文探讨了在印尼绿色革命后循环经济时代背景下,农牧业综合系统作为一种可持续农业解决方案的意义。本研究采用了综合文献综述的方法,包括二手数据分析、研究成果和出版物,以强调综合农作系统在印尼及其他地区的潜力。尽管综合农作法潜力巨大,但印尼农民对其采用有限,原因包括知识和技能不足、政策支持不够以及传播和推广力度不够。为促进综合农作系统的广泛实施,必须采取强有力的推广战略,包括技术指导、咨询、培训和农场示范。此外,政策支持(包括可获得的信贷和价格激励)对于激励农民采用综合农作法也至关重要。本文强调了循环经济学在综合耕作方面的相关性,并为旨在促进印尼后绿色革命时代可持续农业发展的政策制定者和利益相关者提供了见解。
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引用次数: 0
Potential non-carcinogenic and carcinogenic health risks of elements of health concern bioaccumulated in seafood from local fish rafts in Trang province, Thailand 泰国 Trang 省当地鱼排海产品中生物累积的健康问题元素的潜在非致癌和致癌健康风险
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-27 DOI: 10.1016/j.jafr.2024.101272
Kanjana Imsilp , Niyada Lansubsakul , Wachiryah Thong-asa , Pattanasuda Sirinupong , Pun Yeesin , Napasorn Phaochoosak , Phanwimol Tanhan

Marine ecosystems are increasingly contaminated by pollutants from human activities which could impact seafood quality. This study investigated the levels of six potentially toxic elements of health concern including arsenic (As), cadmium (Cd), chromium (Cr), mercury (Hg), nickel (Ni), and lead (Pb). Analyses of those elements were conducted in eleven commercially available seafood species collected from local fish rafts in Trang province, Thailand. The findings revealed variations of element of health concern content among species, with H. raphidea exhibiting significantly high levels. Human health risks were assessed for estimated daily intake and non-carcinogenic and carcinogenic risks. The results indicate potential risks associated with As consumption, especially in the case of H. raphidea. Children (3.0–5.9 years) had the greatest vulnerable risks in comparison to other age groups. The levels of elements of health concern in most seafood samples were within safe limits. However, the continuous surveillance, monitoring, and management of these contaminants in seafood are needed, particularly in vulnerable populations.

海洋生态系统正日益受到人类活动污染物的污染,这可能会影响海产品的质量。本研究调查了与健康有关的六种潜在有毒元素的含量,包括砷(As)、镉(Cd)、铬(Cr)、汞(Hg)、镍(Ni)和铅(Pb)。研究人员对从泰国 Trang 省当地鱼排收集的 11 种市售海产品中的这些元素进行了分析。研究结果表明,不同种类的海产品中与健康有关的元素含量各不相同,其中H. raphidea的含量明显较高。根据估计的每日摄入量以及非致癌和致癌风险,对人类健康风险进行了评估。结果表明,食用 As(尤其是 H. raphidea)会带来潜在风险。与其他年龄组相比,儿童(3.0-5.9 岁)面临的风险最大。大多数海产品样本中与健康有关的元素含量都在安全范围之内。然而,需要对海产品中的这些污染物进行持续监测、监控和管理,尤其是在易感人群中。
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引用次数: 0
Mushroom cultivation in tropical Africa: Successes, challenges, and opportunities 热带非洲的蘑菇种植:成功、挑战和机遇
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-26 DOI: 10.1016/j.jafr.2024.101264
Boukary Aa , Olou Ab , Piepenbring M , Yorou Ns

Mushroom cultivation has become an essential element in the fight against food insecurity, particularly in tropical Africa where agricultural diversity is paramount. Existing literature underlines the importance of mushroom cultivation in improving availability of nutrients and fostering economic resilience. However, cultivation practices still need to be optimized and the diversity of cultivable species native in tropical Africa is largely unknown. This study addresses the role of mushroom cultivation in tropical Africa, its potential and the efforts being made. Data from 93 scientific articles on attempts to cultivate mushrooms in tropical Africa were compiled, revealing the cultivation of 31 fungal species, with Pleurotus ostreatus, Pleurotus tuber-regium, and Lentinus squarrosulus being the most common ones. Despite global popularity, species from genera of Auricularia and Volvariella are rarely cultivated. Wild edible species remain underexplored. The results of this paper also revealed that locally available materials like yams, cassava, and maize are used for media for spawn production. Sorghum grains and sawdust are popular substrates respectively for spawn and fruiting production. Challenges include controlling environmental parameters, limited industrialization due to machinery and technique demands, and enhancing substrate efficiency through supplementation and composting. Temperature and humidity are key factors for successful cultivation, with a temperature range of 20 °C–35 °C considered optimal. Despite the abundance of information regarding mushroom cultivation in tropical Africa, further progress is required to enhance mushroom production techniques.

蘑菇种植已成为应对粮食不安全的重要因素,尤其是在农业多样性至关重要的热带非洲。现有文献强调了蘑菇栽培在改善养分供应和提高经济复原力方面的重要性。然而,栽培方法仍需优化,热带非洲本地可栽培物种的多样性在很大程度上也不为人知。本研究探讨了蘑菇栽培在热带非洲的作用、潜力以及正在做出的努力。研究汇编了 93 篇关于在热带非洲尝试栽培蘑菇的科学文章中的数据,发现了 31 个真菌物种的栽培情况,其中最常见的是 Pleurotus ostreatus、Pleurotus tuber-regium 和 Lentinus squarrosulus。尽管在全球很受欢迎,但 Auricularia 和 Volvariella 属的物种很少被种植。野生食用物种仍未得到充分开发。本文的研究结果还显示,山药、木薯和玉米等当地可用材料被用作产卵介质。高粱粒和锯末分别是产卵和结实的常用基质。面临的挑战包括控制环境参数、因机械和技术要求而限制工业化生产,以及通过补充和堆肥提高基质效率。温度和湿度是成功栽培的关键因素,最佳温度范围为 20 °C-35 °C。尽管有关热带非洲蘑菇栽培的信息非常丰富,但要提高蘑菇生产技术,还需要取得进一步的进展。
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引用次数: 0
Genotypic differences in soybean (Glycine max L.) in yield response to phosphorus fertilizer are associated with difference in biomass and phosphorus content 大豆(Glycine max L.)对磷肥的产量反应基因型差异与生物量和磷含量差异有关
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-26 DOI: 10.1016/j.jafr.2024.101267
Lin-Wei Xu , Jiayin Pang , Neil C. Turner , Hans Lambers , Jin He

The soybean seed yield response to phosphorus (P) fertilizer has significantly increased during soybean breeding but the underlying mechanisms remain elusive. In this study, we used two soybean cultivars with high (Andou 8 (AD8)) and low (Qiandou 5 (QD5)) seed yield response to P fertilizer to compare their yield and yield components, biomass accumulation and partitioning, P content and partitioning and P-use efficiency in Shiqian (SQ) and Dafang (DF) during the growing season in 2020 under 0 (P0) and 35 (P35) kg P ha−1 supply. The results show that AD 8 had a greater seed yield, pod biomass, filled-pod number, seed number, pod P content and P partitioning to pods, and P-use efficiency based on seed yield (PUESY) but lower P-use efficiency based on biomass (PUEB) than QD5 at two experimental sites. The seed number was positively correlated with total biomass and P content, especially with greater pod biomass accumulation and its partition to pods which showed a positive correlation with seed number. Seed number was negatively correlated with stem dry weight and its partitioning to stem, and stem P partition. We conclude (1) different yield response to P fertilizer are explained by different biomass and P content and their partitioning to pod; (2) high biomass and P-conversion efficiency increased the seed number, which accounted for a high yield response to P fertilizer. Our results highlight that soybean breeding increased biomass and nutrient content and their conversion efficiency to achieve high seed yield with P-fertilizer supply.

在大豆育种过程中,大豆籽粒产量对磷肥的响应显著提高,但其潜在机制仍不清楚。本研究利用安豆 8 号(AD8)和黔豆 5 号(QD5)这两个对磷肥响应较高的大豆品种,比较了 2020 年石阡(SQ)和大方(DF)大豆生长季在 0(P0)和 35(P35) kg P ha-1 供肥条件下的产量和产量组成、生物量积累和分配、磷含量和分配以及磷利用效率。结果表明,与 QD5 相比,AD 8 在两个试验点的种子产量、荚果生物量、饱满荚果数、种子数、荚果 P 含量和荚果 P 分配以及基于种子产量的 P 利用效率(PUESY)都更高,但基于生物量的 P 利用效率(PUEB)较低。种子数与总生物量和钾含量呈正相关,尤其是豆荚生物量积累和豆荚钾分配与种子数呈正相关。种子数与茎干重及其在茎中的分配和茎钾分配呈负相关。我们的结论是:(1)不同的生物量和钾含量及其在豆荚中的分配情况解释了对钾肥的不同产量响应;(2)高生物量和钾转化效率增加了种子数,这是对钾肥的高产响应的原因。我们的研究结果突出表明,大豆育种提高了生物量和养分含量及其转化效率,从而在提供钾肥的情况下实现了高产。
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引用次数: 0
Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers 过氧乙酸在细菌缓冲溶液中和甜椒上抗李斯特菌的功效
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-24 DOI: 10.1016/j.jafr.2024.101265
Peighton Foster, Corey Coe, Carly Long, Md Shafiul Islam Rion, Gary Freshour, Annette Freshour, Jacek Jaczynski, Kristen Matak, Cangliang Shen

This study aims to 1) evaluate the efficacy of peroxyacetic acid (PAA) to inactivate Listeria monocytogenes in 0.1 % buffered-peptone-water; and 2) evaluate the reduction and mitigation of cross-contamination on peppers. In study-1, aliquots of 1.0-ml PAA solutions (133, 265, and 448 ppm) were added to the first 6 wells of 8-strip-deep well microplates. Then, 0.1 ml of serially diluted nalidixic-acid-resistant (NaL) L. monocytogenes was added and mixed immediately with a multichannel pipette. After exposure for 0, 5, 15, 30, and up to 120-s, 1 ml of 2 × D/E neutralized solution was added to terminate the reaction followed by spread-plating onto tryptic soy agar +200 ppm NaL and incubated at 35 °C for 48 h. In study-2, 5 inoculated red peppers were triple-washed with 15 uninoculated green peppers with 0, 23, 53, and 96 ppm of PAA and analyzed using MPN-method. L. monocytogenes counts were <0.3 log10 CFU/ml after exposure to 133, 265, and 448 ppm of PAA for 120, 90 and 60 s, respectively and fit the Linear and Weibull Models. Applying 23–96 ppm of PAA reduced L. monocytogenes by 2.03–3.32 log10MPN/g and transferred cross-contaminated cell counts by 2.36–3.17 log10MPN/g, with no differences (P > 0.05) compared to the water only treatment. Results suggested that applying allowable concentrations (23–96 ppm) of PAA during triple-wash does not show promising anti-Listeria activity on peppers. Future studies are needed to determine the minimum concentrations of the PAA solution required to effectively reduce and mitigate microbial cross-contamination of Listeria monocytogenes on peppers.

本研究旨在:1)评估过氧乙酸(PAA)灭活 0.1 % 缓冲蛋白胨水中的李斯特菌的效果;2)评估减少和减轻辣椒交叉污染的效果。在研究-1 中,将 1.0 毫升 PAA 溶液(133、265 和 448 ppm)的等分试样加入 8 带深孔微孔板的前 6 个孔中。然后,加入 0.1 毫升经序列稀释的耐萘啶酸性(NaL)单核细胞增多性乳酸杆菌,并立即用多通道移液管混合。暴露 0、5、15、30 至 120 秒后,加入 1 毫升 2 × D/E 中和溶液终止反应,然后平铺到胰蛋白酶大豆琼脂 +200 ppm NaL 上,在 35 °C 下培养 48 小时。在分别暴露于 133、265 和 448 ppm PAA 120、90 和 60 秒后,单核细胞增多症菌落总数分别为 0.3 log10 CFU/ml,并符合线性和威布尔模型。与仅用水处理相比,使用 23-96 ppm PAA 可使单核细胞增多症菌落总数减少 2.03-3.32 log10MPN/g,交叉污染细胞数减少 2.36-3.17 log10MPN/g,且无差异(P > 0.05)。结果表明,在三重清洗过程中使用允许浓度(23-96 ppm)的 PAA 对辣椒的抗李斯特菌活性并不乐观。未来的研究需要确定 PAA 溶液的最低浓度,以有效减少和减轻辣椒上李斯特菌的微生物交叉感染。
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引用次数: 0
Aroma and metabolite profiling in duckweeds: Exploring species and ecotypic variation to enable wider adoption as a food crop 浮萍的芳香和代谢物分析:探索物种和生态型变异,以便更广泛地将其作为一种粮食作物
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-22 DOI: 10.1016/j.jafr.2024.101263
Kellie E. Smith , Martin Schäfer , Mui Lim , Carlos A. Robles-Zazueta , Laura Cowan , Ian D. Fisk , Shuqing Xu , Erik H. Murchie

Duckweeds (water lentils) are a nutritious human food source, with Wolffia species consumed traditionally in Eastern Asia. Duckweed contain up to 45 % protein by dry weight, high macronutrients, minerals and carotenoids. However, duckweed are not cultivated at scale and there are circa 35 other species to consider for food potential in other global regions. Here, we measured the suitability of four Lemna species and Spirodela polyrhiza for nutritional assessment, by scaling up growth of 25 ecotypes from the United Kingdom in a glasshouse. Here we showed intra- and inter-species variation of aromatic and metabolic profiles, together with biomass obtained from production. The dominant volatile organic compounds (VOCs) in duckweed are hexanal, 1-penten-3-one, 1-penten-3-ol, cis-2-pentanol and pentadecanal, with variations in amounts of 22 other compounds between species. In comparison with other leafy herbs, duckweed aroma profiles were most similar to spinach and dandelion with high ‘green’ and ‘fresh’ aroma compounds. Spirodela polyrhiza contained high flavonoids including apigenin and luteolin, offering potential benefits for health. Our results demonstrate that Lemna and Spirodela species have suitable flavonoid and amino acid profiles for nutrition. VOCs found here had positive aroma descriptors and can be used as biomarkers of freshness during storage of duckweed foodstuffs.

浮萍(扁豆)是一种营养丰富的人类食物来源,在东亚,人们传统上食用 Wolffia 品种。按干重计算,浮萍含有高达 45% 的蛋白质、大量常量营养素、矿物质和类胡萝卜素。然而,浮萍并没有大规模种植,在全球其他地区还有大约 35 种浮萍可供食用。在这里,我们通过扩大英国 25 种生态型浮萍在玻璃温室中的生长规模,测量了四种 Lemna 和 Spirodela polyrhiza 的营养评估适宜性。在这里,我们展示了芳香和代谢特征的种内和种间差异,以及从生产中获得的生物量。浮萍中最主要的挥发性有机化合物(VOCs)是己醛、1-戊烯-3-酮、1-戊烯-3-醇、顺式-2-戊醇和十五醛,其他 22 种化合物的含量在不同物种之间存在差异。与其他多叶草本植物相比,浮萍的香气特征与菠菜和蒲公英最为相似,具有较高的 "绿色 "和 "新鲜 "香气化合物。Spirodela polyrhiza 含有大量类黄酮,包括芹菜素和叶黄素,具有潜在的健康益处。我们的研究结果表明,Lemna 和 Spirodela 物种的类黄酮和氨基酸含量适合作为营养品。这里发现的挥发性有机化合物具有积极的芳香描述,可用作浮萍食品储存期间新鲜度的生物标记。
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引用次数: 0
Effects of cover crops and nitrogen fertilizer on greenhouse gas emissions and net global warming potential in a potato cropping system 覆盖作物和氮肥对马铃薯种植系统温室气体排放和全球变暖净潜势的影响
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-06-19 DOI: 10.1016/j.jafr.2024.101256
Shayesteh Sadra, Gholamreza Mohammadi, Farzad Mondani

The study was conducted to investigate the effects of cover crops and different nitrogen levels on greenhouse gas emissions and net global warming potential in a potato production system during the 2018–2019 and 2019–2020 growing seasons at the Agricultural Research Farm of Razi University, Kermanshah, Iran. The experiment was arranged as a split plot based on a randomized complete block design with three replications. The main factor was cover crop including hairy vetch, rye, mixed (rye + hairy vetch), and control (no cover crop), and the sub factor was nitrogen fertilizer (as urea) at four levels consisted of 0 (control), 33, 66 and 100 % of the recommended nitrogen fertilizer based on the soil test. Results showed that the use of cover crops as green manures increased net ecosystem carbon balance (NECB) and the highest NECB was observed in the mixed treatment (rye + hairy vetch) along with the 66 % of nitrogen fertilizer. The mixed cover crops (hairy vetch + rye) along with the 100 % of nitrogen fertilizer showed higher CO2 emission and, while hairy vetch along with the 100 % of nitrogen fertilizer had higher emissions of N2O and CH4 during the potato growing season. It can be concluded that the use of cover crops as green manures before the planting of potato can notably increase greenhouse gas emissions and the net global warming.

本研究旨在调查伊朗克尔曼沙阿市拉齐大学农业研究农场的马铃薯生产系统中,在2018-2019年和2019-2020年生长季期间,覆盖作物和不同氮素水平对温室气体排放和净全球升温潜能值的影响。实验采用随机完全区组设计,三次重复,分小区进行。主因子为覆盖作物,包括毛茸茸的 vetch、黑麦、混合(黑麦 + 毛茸茸的 vetch)和对照(无覆盖作物);副因子为氮肥(尿素),有四个水平,包括 0(对照)、33%、66% 和 100%,均为根据土壤测试推荐的氮肥。结果表明,使用覆盖作物作为绿肥增加了生态系统净碳平衡(NECB),在混合处理(黑麦+毛茸茸绒草)和 66% 的氮肥中观察到的净碳平衡最高。在马铃薯生长季节,混合覆盖作物(毛绒绒vetch + 黑麦)和 100 % 氮肥显示出较高的二氧化碳排放量,而毛绒绒vetch 和 100 % 氮肥则显示出较高的一氧化二氮(N2O)和甲烷(CH4)排放量。由此可以得出结论,在马铃薯种植前使用覆盖作物作为绿肥会显著增加温室气体排放量和全球变暖净值。
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引用次数: 0
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Journal of Agriculture and Food Research
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