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Investigating the impact of the key processing stages on flavor components and lipid flavor formation of Tibetan dried pork slice 研究了关键加工阶段对藏式干猪肉片风味成分及脂质风味形成的影响
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-17 DOI: 10.1016/j.jafr.2026.102696
Xue Xiao , Huaying Chen , Xuehai Ge , Jiangping Fan , Liangyu Yang , Junhua Shao , Shijun Li , Changrong Ge , Zhichao Xiao
This study aimed to explore the aroma formation mechanism of Tibetan dried pork slice by investigating changes of their volatile compounds and lipid molecules across four key processing stages (mincing, seasoning, drying, and baking). E-nose analysis revealed significant differences in the odor profiles of dried pork slice at different processing stages. Furthermore, the drying and baking processes significantly enhanced the odor profiles of dried pork slice. The GC-IMS detected 61 volatile compounds, among which PLS-DA highlighted nine key contributors to the final flavor profile: ethyl 2-hydroxypropanoate-M, butanal, propanal, 3-methyl butanal, benzaldehyde, 1-nonanal, 1-hexanal-D, (Z)-2-pentenal, and 2-methylpyrazine. Additionally, the four processes significantly changed the content and composition of lipid molecules analyzed through LC-MS. Glycerolipids and phospholipids metabolism were identified as the primary metabolic pathway during the processing stages. Triglycerides (TG) and phosphatidylcholine (PC), such as TG (13:1/13:1/13:1), TG (17:0/18:1/18:1), TG (13:1/21:4/21:4), TG (12:0/12:0/19:3), TG (16:0/22:1/22:1), PC (18:4e/18:5) and PC (18:3/22:6), played a vital role in the generation of aroma compounds. Furthermore, the correlation between the lipid molecules and the key volatile compounds suggested the role of lipid oxidation, the Maillard reaction, as well as the interaction between these reactions in the aroma formation of dried pork slice. Therefore, this study provides the first evidence that lipid oxidation in the drying and baking stages is a critical factor in the aroma evolution of Tibetan dried pork slice. These findings provide a scientific basis for understanding the flavor formation mechanism and inform the development of optimized processing strategies for Tibetan dried pork slice.
本研究旨在通过研究藏肉干片在剁碎、调味、干燥和烘烤四个关键加工阶段挥发性化合物和脂质分子的变化,探讨藏肉干片的香气形成机制。电子鼻分析表明,不同加工阶段干猪肉片的气味特征存在显著差异。此外,干燥和烘烤过程显著增强了干猪肉片的气味特征。GC-IMS检测到61种挥发性化合物,其中PLS-DA突出了9种影响最终风味特征的关键化合物:2-羟丙酸乙酯-m、丁醛、丙醛、3-甲基丁醛、苯甲醛、1-壬醛、1-己醛- d、(Z)-2-戊烯醛和2-甲基吡嗪。此外,这四个过程显著改变了LC-MS分析的脂质分子的含量和组成。甘油脂和磷脂代谢被确定为加工阶段的主要代谢途径。甘油三酯(TG)和磷脂酰胆碱(PC),如TG(13:1/13:1/13:1)、TG(17:0/18:1/18:1)、TG(13:1/21:4/21:4)、TG(12:0/12:0/19:3)、TG(16:0/22:1/22:1)、PC (18:4e/18:5)和PC(18:3/22:6)在香气化合物的生成中起着至关重要的作用。此外,脂质分子与关键挥发性化合物之间的相关性表明,脂质氧化、美拉德反应以及这些反应之间的相互作用在干猪肉片香气形成中的作用。因此,本研究首次证明了干燥和烘烤阶段的脂质氧化是藏式干猪肉片香气演变的关键因素。这些研究结果为了解藏式干猪肉风味形成机制提供了科学依据,并为制定藏式干猪肉片的优化加工策略提供了依据。
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引用次数: 0
Pesticide handling practices, occupational risks, and health expenses among Bangladeshi smallholder vegetable farmers 孟加拉国蔬菜小农的农药处理做法、职业风险和保健费用
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-28 DOI: 10.1016/j.jafr.2026.102727
Md. Shakhawat Hossain , Shah Fahad , Tanwne Sarker , Md. Ghulam Rabbany , Sabina Yeasmin , Rana Roy
Overlooking fundamental safety measures when applying pesticides in agriculture endangers farmers' health, increases healthcare expenses, and harms the environment. It's well-documented that Bangladeshi farmers use pesticides without taking enough precautions. Employing cross-sectional data, this study utilizes the theory of averting behavior model to assess pesticide handling practices, occupational hazards, and health expenditures among smallholder vegetable producers. While previous Bangladeshi studies focused solely on farmers' attitudes, knowledge, and practices regarding the use of pesticides, the current research identifies a critical gap and presents some new insights. The findings reveal that the application of safety equipment and pesticide container disposal methods was significantly influenced by farmers' investments in health and safety measures (P < 0.05) and the health consequences (P < 0.05) they faced. Farmers spent 4.21 US dollars per farm on health and safety measures against pesticide exposure during the 2023 vegetable season. Most farmers were unaware of properly managing pesticide equipment and disposal techniques for pesticide containers. The logit model results also reveal that farmers were more likely to adhere to precautionary measures and dispose of empty pesticide containers correctly as their age, educational level, and participation in the IPM (Integrated Pest Management) training program increased. These findings emphasize the need for practical IPM training and farmers' educational programs to ensure safe pesticide use. Findings can assist policymakers in understanding the reasons behind the improper application and disposal of pesticide containers. Moreover, the research suggests improved pesticide handling practices can boost sustainable agriculture and reduce health and occupational risks in Bangladesh.
在农业中使用农药时忽视基本的安全措施,危害农民的健康,增加医疗费用,危害环境。有充分的证据表明,孟加拉国农民在没有采取足够的预防措施的情况下使用杀虫剂。本研究采用横截面数据,运用规避行为模型理论,评估小农蔬菜生产者的农药处理方式、职业危害和卫生支出。虽然孟加拉国以前的研究只关注农民对农药使用的态度、知识和做法,但目前的研究发现了一个关键的差距,并提出了一些新的见解。结果表明,安全设备的使用和农药容器处置方式显著影响农民在健康和安全措施上的投入(P < 0.05)和他们面临的健康后果(P < 0.05)。在2023年蔬菜季节,农民在每个农场花费4.21美元用于防止农药暴露的健康和安全措施。大多数农民不知道如何正确管理农药设备和农药容器的处理技术。logit模型结果还显示,随着年龄、受教育程度和参加有害生物综合治理(IPM)培训计划的增加,农民更有可能坚持采取预防措施并正确处理空农药容器。这些研究结果强调了切实可行的IPM培训和农民教育计划的必要性,以确保农药的安全使用。研究结果可以帮助决策者了解不当使用和处置农药容器背后的原因。此外,该研究表明,改进农药处理方法可以促进孟加拉国的可持续农业,减少健康和职业风险。
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引用次数: 0
Investigation of machine learning based techniques for crop yield estimation of corn and cotton using multi-sensor data fusion 基于机器学习的多传感器数据融合玉米和棉花产量估算技术研究
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-26 DOI: 10.1016/j.jafr.2026.102676
Mohammad Abdus Shahid Rafi , Volkan Senyurek , Ardeshir Adeli , Huang Yanbo , John E. Ball , Ali C. Gurbuz
Accurate crop yield estimation is crucial for decision-making and planning in modern agriculture with increasing challenges with food security. Yield predictions provide farmers with insights into expected production, facilitating optimized resource allocation, improved agricultural management strategies, and enhanced profitability. This study investigates the application of machine learning (ML) techniques, including Feedforward Neural Networks (FNN), Long Short-Term Memory (LSTM), and Random Forest (RF) models, for predicting crop yields using multi-sensory time-series data that has been collected on two fields over a four-year timeframe. The focus is on corn (Zea mays) and cotton (Gossypium hirsutum) yield, two of the top critical crops in Mississippi region. A multi-sensory dataset was collected using multispectral cameras and LiDAR sensors mounted on unmanned aircraft systems (UAS), along with soil moisture and temperature data from volumetric probes and environmental data from a nearby weather station. Over four years, more than 30 features were extracted weekly from five major categories, with 235 ground truth yield records from plots in the field. The study outlines the methodology for feature selection and examines its impact on yield prediction accuracy. Using percentile root mean square error (RRMSE) and mean absolute percentage error (MAPE) as performance metrics, the study found that the proposed LSTM model produced lower field-wise errors (9 − 21 % MAPE) compared to other models and validation, indicating superior performance in predicting yields across selected weeks. The proposed ML-based approach, validated through year-based and field-wise cross-validation methods, demonstrates the effectiveness of using UAS-collected multi-sensor data for accurate yield estimation in corn and cotton.
在粮食安全面临日益严峻挑战的现代农业中,准确的作物产量估算对农业决策和规划至关重要。产量预测为农民提供了对预期产量的洞察,促进了资源的优化配置,改进了农业管理策略,提高了盈利能力。本研究探讨了机器学习(ML)技术的应用,包括前馈神经网络(FNN)、长短期记忆(LSTM)和随机森林(RF)模型,利用在四年时间框架内从两个领域收集的多感官时间序列数据预测作物产量。重点是玉米(Zea mays)和棉花(Gossypium hirsutum)的产量,这是密西西比地区最重要的两种作物。使用安装在无人机系统(UAS)上的多光谱相机和激光雷达传感器收集了多传感器数据集,以及来自体积探测器的土壤湿度和温度数据以及来自附近气象站的环境数据。在四年的时间里,每周从五个主要类别中提取30多个特征,从实地地块中提取235个地面真实产量记录。该研究概述了特征选择的方法,并考察了其对产量预测精度的影响。使用百分位均方根误差(RRMSE)和平均绝对百分比误差(MAPE)作为性能指标,研究发现,与其他模型和验证相比,所提出的LSTM模型产生了更低的田间误差(9 - 21% MAPE),表明在预测选定周的产量方面具有优越的性能。本文提出的基于机器学习的方法,经过了基于年份和田间交叉验证方法的验证,证明了使用无人机收集的多传感器数据准确估计玉米和棉花产量的有效性。
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引用次数: 0
Repurposing the human antiviral phytochemical drug, agathisflavone, for management of begomoviruses infecting plants 重新利用人类抗病毒植物化学药物agathisflavone,用于管理感染植物的begomovirus
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2025-12-30 DOI: 10.1016/j.jafr.2025.102624
Mehulee Sarkar , Firoz Mondal , Dipsikha Mondal , Shyam Kumar Gupta , Yeluru Mohan Babu , Anik Majumdar , Supradip Saha , Sneha Murmu , Mahender Singh , Girish Kumar Jha , Bikash Mandal , Anirban Roy

Background

Begomoviruses are whitefly-transmitted circular single-stranded DNA viruses causing severe yield losses in economically important crops, particularly in tropical and subtropical regions. Current management strategies depend mainly on pesticide-based vector control and host resistance, which are limited by environmental concerns and frequent resistance breakdown due to rapid viral evolution. This study aimed to identify and validate a small-molecule inhibitor targeting the replication initiator protein (Rep) of tomato leaf curl New Delhi virus (ToLCNDV), a major begomovirus infecting tomato.

Methods

Fifteen plant-derived phytochemicals with reported antiviral activity against human viruses were screened in silico against the ToLCNDV Rep protein. Molecular docking and molecular dynamics simulations were used to evaluate binding affinity and stability, while protein–DNA docking assessed overlap between inhibitor-binding residues and the viral DNA-binding domain. As agathisflavone showed the most stable binding with Rep, it was isolated from cashew (Anacardium occidentale) leaves and formulated as an agathisflavone-enriched formulation (AGT-F). AGT-F was applied as a foliar spray and seed treatment, alone or in combination, under different inoculation regimes. Antiviral efficacy was assessed through symptom development, viral load estimation, and oxidative stress analysis.

Results

Agathisflavone emerged as the lead compound, showing high binding affinity (−8.6 kcal/mol) toward Rep and stable interaction during a 100 ns molecular dynamics simulation. Protein–DNA docking revealed overlapping residues between the agathisflavone-binding site and the viral DNA-binding domain of Rep. In planta studies showed delayed symptom development up to 20 days post-inoculation (dpi), compared to 8 dpi in control plants, and up to 99 % reduction in viral accumulation following combined seed treatment and foliar spray. AGT-F also reduced virus-induced oxidative stress and improved antioxidant status and seedling vigour.

Conclusion

This study demonstrates cross-kingdom repurposing of agathisflavone as a promising eco-friendly antiviral molecule targeting the Rep protein of ToLCNDV, providing a rational strategy for begomovirus disease management.
begomovirus是一种由白蛉传播的环状单链DNA病毒,对经济上重要的作物造成严重的产量损失,特别是在热带和亚热带地区。目前的管理策略主要依赖于基于杀虫剂的媒介控制和宿主抗性,这受到环境问题和病毒快速进化导致的抗性频繁破坏的限制。本研究旨在鉴定和验证一种靶向番茄叶片卷曲新德里病毒(ToLCNDV)复制启动蛋白(Rep)的小分子抑制剂。方法从已有报道的15种具有抗病毒活性的植物源性植物化学物质中筛选抗人病毒的ToLCNDV Rep蛋白。分子对接和分子动力学模拟用于评估结合亲和力和稳定性,而蛋白质- dna对接用于评估抑制剂结合残基与病毒dna结合结构域之间的重叠。由于agathisflavone与Rep结合最稳定,因此从腰果(Anacardium occidentale)叶片中分离得到agathisflavone富集制剂(AGT-F)。AGT-F作为叶面喷雾和种子处理,在不同接种制度下单独或联合施用。通过症状发展、病毒载量估计和氧化应激分析来评估抗病毒疗效。结果agathisflavone为先导化合物,对Rep具有较高的结合亲和力(−8.6 kcal/mol),在100 ns分子动力学模拟中相互作用稳定。蛋白质- dna对接发现,在无糖黄酮结合位点和病毒dna结合域之间存在重叠残基。在植物研究中,与对照植物的8 dpi相比,接种后20天的症状发展延迟,并且在种子处理和叶面喷雾联合处理后,病毒积累减少了99%。AGT-F还能降低病毒诱导的氧化应激,提高抗氧化状态和幼苗活力。结论agathisflavone可作为一种靶向ToLCNDV Rep蛋白的有前途的生态友好型抗病毒分子,为begomovirus疾病的防治提供了合理的策略。
{"title":"Repurposing the human antiviral phytochemical drug, agathisflavone, for management of begomoviruses infecting plants","authors":"Mehulee Sarkar ,&nbsp;Firoz Mondal ,&nbsp;Dipsikha Mondal ,&nbsp;Shyam Kumar Gupta ,&nbsp;Yeluru Mohan Babu ,&nbsp;Anik Majumdar ,&nbsp;Supradip Saha ,&nbsp;Sneha Murmu ,&nbsp;Mahender Singh ,&nbsp;Girish Kumar Jha ,&nbsp;Bikash Mandal ,&nbsp;Anirban Roy","doi":"10.1016/j.jafr.2025.102624","DOIUrl":"10.1016/j.jafr.2025.102624","url":null,"abstract":"<div><h3>Background</h3><div>Begomoviruses are whitefly-transmitted circular single-stranded DNA viruses causing severe yield losses in economically important crops, particularly in tropical and subtropical regions. Current management strategies depend mainly on pesticide-based vector control and host resistance, which are limited by environmental concerns and frequent resistance breakdown due to rapid viral evolution. This study aimed to identify and validate a small-molecule inhibitor targeting the replication initiator protein (Rep) of tomato leaf curl New Delhi virus (ToLCNDV), a major begomovirus infecting tomato.</div></div><div><h3>Methods</h3><div>Fifteen plant-derived phytochemicals with reported antiviral activity against human viruses were screened <em>in silico</em> against the ToLCNDV Rep protein. Molecular docking and molecular dynamics simulations were used to evaluate binding affinity and stability, while protein–DNA docking assessed overlap between inhibitor-binding residues and the viral DNA-binding domain. As agathisflavone showed the most stable binding with Rep, it was isolated from cashew (<em>Anacardium occidentale</em>) leaves and formulated as an agathisflavone-enriched formulation (AGT-F). AGT-F was applied as a foliar spray and seed treatment, alone or in combination, under different inoculation regimes. Antiviral efficacy was assessed through symptom development, viral load estimation, and oxidative stress analysis.</div></div><div><h3>Results</h3><div>Agathisflavone emerged as the lead compound, showing high binding affinity (−8.6 kcal/mol) toward Rep and stable interaction during a 100 ns molecular dynamics simulation. Protein–DNA docking revealed overlapping residues between the agathisflavone-binding site and the viral DNA-binding domain of Rep. <em>In planta</em> studies showed delayed symptom development up to 20 days post-inoculation (dpi), compared to 8 dpi in control plants, and up to 99 % reduction in viral accumulation following combined seed treatment and foliar spray. AGT-F also reduced virus-induced oxidative stress and improved antioxidant status and seedling vigour.</div></div><div><h3>Conclusion</h3><div>This study demonstrates cross-kingdom repurposing of agathisflavone as a promising eco-friendly antiviral molecule targeting the Rep protein of ToLCNDV, providing a rational strategy for begomovirus disease management.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102624"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145895945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of high temperature hot-air blanching pretreatment on the quality of shiitake mushrooms under different hot-air drying modes 高温热风漂白预处理对不同热风干燥方式下香菇品质的影响
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-08 DOI: 10.1016/j.jafr.2026.102652
Min Tan , Ling Tang , Yu Chen , Hui Zeng , Shaobin Fu , Xiaogang Zhang
The low input and easily controlled hot-air drying (HAD) is the most widely used drying technology, while the quality of shiitake mushrooms varied with different drying temperature or pretreatment modes, driving exploration of environmentally friendly drying strategy that is conducive to product quality. The focus of this study is the effects of high temperature hot-air blanching pretreatment (100 °C, 10 min) combined with different HAD-modes (constant temperature at 35 and 65 °C, and temperature gradient at 35–65 °C) on quality of shiitake mushrooms. Overall, high temperature hot-air blanching induced darker color and severe shrinkage, caused higher extent of browning and cross-linking and aggregation of proteins, and reduced the chemical antioxidant capacities in mushrooms. For unblanched samples, compared to those dried at constant temperature of 35 and 65 °C, the sample under temperature gradient at 35–65 °C (G) showed lower browning degree based on color index and the absorbance at 420 nm, and a better appearance was also observed. The higher chemical antioxidant capacities were observed in G containing higher contents of free amino group and total phenols. The protein molecular weights of G were mainly ranged at 50–70 kDa, and proteins below 50 kDa were also widely distributed. In addition, the amino acid patterns suggested G had high-quality protein, which was rich in free amino acids with sweetness and umami. Thus, hot-air blanching may not contribute to the quality of dried shiitake mushrooms, and temperature gradient HAD without blanching could be a practical approach for drying of shiitake mushrooms.
低投入、易控制的热风干燥(HAD)是目前应用最广泛的干燥技术,而香菇的品质随干燥温度或预处理方式的不同而不同,这推动了有利于产品质量的环保干燥策略的探索。本研究的重点是高温热风焯水预处理(100℃,10 min)结合不同的高温预处理模式(35℃和65℃恒温,35 ~ 65℃温度梯度)对香菇品质的影响。总体而言,高温热风焯水导致蘑菇颜色变深,收缩严重,褐变程度提高,蛋白质交联和聚集程度提高,化学抗氧化能力降低。对于未漂白的样品,与35℃和65℃恒温干燥的样品相比,35 - 65℃(G)温度梯度下的样品在显色指数和420 nm吸光度上的褐变程度较低,外观也更好。游离氨基和总酚含量较高的G具有较高的化学抗氧化能力。G蛋白分子量主要在50 - 70 kDa之间,50 kDa以下的蛋白也广泛分布。此外,氨基酸图谱表明G具有优质蛋白质,富含游离氨基酸,具有甜味和鲜味。因此,热风热烫对香菇干燥质量影响不大,不热烫的温度梯度HAD可能是一种实用的香菇干燥方法。
{"title":"Effect of high temperature hot-air blanching pretreatment on the quality of shiitake mushrooms under different hot-air drying modes","authors":"Min Tan ,&nbsp;Ling Tang ,&nbsp;Yu Chen ,&nbsp;Hui Zeng ,&nbsp;Shaobin Fu ,&nbsp;Xiaogang Zhang","doi":"10.1016/j.jafr.2026.102652","DOIUrl":"10.1016/j.jafr.2026.102652","url":null,"abstract":"<div><div>The low input and easily controlled hot-air drying (HAD) is the most widely used drying technology, while the quality of shiitake mushrooms varied with different drying temperature or pretreatment modes, driving exploration of environmentally friendly drying strategy that is conducive to product quality. The focus of this study is the effects of high temperature hot-air blanching pretreatment (100 °C, 10 min) combined with different HAD-modes (constant temperature at 35 and 65 °C, and temperature gradient at 35–65 °C) on quality of shiitake mushrooms. Overall, high temperature hot-air blanching induced darker color and severe shrinkage, caused higher extent of browning and cross-linking and aggregation of proteins, and reduced the chemical antioxidant capacities in mushrooms. For unblanched samples, compared to those dried at constant temperature of 35 and 65 °C, the sample under temperature gradient at 35–65 °C (G) showed lower browning degree based on color index and the absorbance at 420 nm, and a better appearance was also observed. The higher chemical antioxidant capacities were observed in G containing higher contents of free amino group and total phenols. The protein molecular weights of G were mainly ranged at 50–70 kDa, and proteins below 50 kDa were also widely distributed. In addition, the amino acid patterns suggested G had high-quality protein, which was rich in free amino acids with sweetness and umami. Thus, hot-air blanching may not contribute to the quality of dried shiitake mushrooms, and temperature gradient HAD without blanching could be a practical approach for drying of shiitake mushrooms.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102652"},"PeriodicalIF":6.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145928799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Global research hotspots and trends in polysaccharide anti-tumor therapy: A systematic bibliometric analysis and review 多糖抗肿瘤治疗的全球研究热点与趋势:系统文献计量学分析与综述
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-10 DOI: 10.1016/j.jafr.2026.102635
Yuyang Su , Weiqing Qian , Xiaohan Cao , Hongying Wang , Xiaocan Lei , Weidong Gong , Jialin Hu

Objective

Polysaccharides, as natural bioactive macromolecules with anti-tumor potential, have become a research hotspot in the context of limited traditional cancer treatment methods. However, there is a lack of systematic reviews and clear delineation of future trends in this field regarding polysaccharide-based anti-tumor therapy. To fill this gap, this study aims to analyze the research hotspots and trends using bibliometric methods, providing support for promoting clinical translation.

Method

Relevant literature was retrieved from the Web of Science database, and key indicators such as annual publication volume, national/institutional collaboration models, and research hotspots were visually analyzed.

Result

The global publications on polysaccharide-based anti-tumor therapy have been on the rise. China, the United States, and India are the top three countries in terms of output. The research hotspots can be clustered into three major directions: “Polysaccharide-based nanocarriers and tumor-targeted delivery”, “Polysaccharide-mediated drug delivery systems and efficacy enhancement”, and “Polysaccharide anti-tumor mechanisms and tumor treatment effects”. Current research focuses on nanocarrier construction, targeted delivery mechanisms, and exploration of the anti-tumor activity of natural polysaccharides.

Conclusion

Research on polysaccharide-based anti-tumor therapy is steadily developing and has a cross-disciplinary characteristic. Based on the analysis of the research dynamics in this field, this study not only further validates and highlights the anti-tumor potential of polysaccharides, but also clearly indicates that future research will focus on precision medicine, combined treatment, and green synthesis technologies, providing key references for promoting clinical innovation and transformation in tumor treatment.
目的多糖作为具有抗肿瘤潜力的天然生物活性大分子,在传统肿瘤治疗方法有限的情况下成为研究热点。然而,目前缺乏系统的综述和对该领域以多糖为基础的抗肿瘤治疗的未来趋势的明确描述。为了填补这一空白,本研究旨在利用文献计量学方法分析研究热点和趋势,为促进临床翻译提供支持。方法从Web of Science数据库中检索相关文献,可视化分析年发表量、国家/机构合作模式、研究热点等关键指标。结果全球多糖类抗肿瘤药物的研究文献呈上升趋势。中国、美国和印度是产量最高的三个国家。研究热点可集中在“基于多糖的纳米载体与肿瘤靶向递送”、“多糖介导的药物递送系统与功效增强”和“多糖抗肿瘤机制与肿瘤治疗作用”三大方向。目前的研究主要集中在天然多糖的纳米载体构建、靶向递送机制以及抗肿瘤活性的探索等方面。结论以多糖为基础的抗肿瘤治疗研究正在稳步发展,具有跨学科的特点。在分析该领域研究动态的基础上,本研究不仅进一步验证和凸显了多糖的抗肿瘤潜力,也明确了未来的研究方向将集中在精准医疗、联合治疗、绿色合成技术等方面,为推动肿瘤治疗的临床创新和转型提供关键参考。
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引用次数: 0
Analyzing meteorological effects on crop yield and yield prediction through machine learning with different data partitioning approaches: A case study from Czech Republic 通过不同数据划分方法的机器学习分析气象对作物产量和产量预测的影响:以捷克共和国为例
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-10 DOI: 10.1016/j.jafr.2026.102662
Bojana Petrovic , Daniel Paluba , Adenan Yandra Nofrizal , Ales Kucera
Climate change and increasing weather variability pose significant challenges to agricultural productivity, particularly in temperate regions. This study examines climate impacts on yields of barley, rapeseed, rye, and wheat across the Czech Republic (2016–2024) using meteorological data and evaluates three machine learning models (Random Forest, Gradient Boosting, Support Vector Regression) for yield prediction solely on meteorological variables. Three validation strategies were tested: random (70/30 %), spatial (region-based), and temporal (last three years). Random partitioning yielded the highest accuracy, but risks data leakage and is therefore not recommended for forecasting. Spatial partitioning showed low to moderate accuracy, suggesting ML approaches can partially interpolate yields in areas with no data (interpolation), particularly for barley and wheat. Temporal partitioning caused a substantial drop in predictive skill, producing unreliable forecasts for rye and rapeseed and indicating that meteorological data alone are insufficient for robust yield prediction. Support Vector Regression produced consistently negative R2 values across all validation strategies, demonstrating fundamental model-task incompatibility. Wheat showed highest accuracy (random: 72.57 ± 7.19 %; spatial: 53.45 ± 19.31 %; temporal: 25.10 %), driven largely by Year (>50 % importance). Without Year, spatial R2 dropped to 0.2–27 %, confirming limited predictive power of meteorological variables alone. Crop climate analysis revealed distinct responses: rye was highly heat-sensitive, wheat and rapeseed benefited from late-season warmth, excess soil moisture during dormancy reduced rapeseed and wheat yields, and barley responded positively to stable warm conditions. These findings emphasize the importance of choosing an appropriate validation approach for yield prediction, depending on the intended task (interpolation or forecasting), as well as the value of feature interpretability. Key limitations include reliance on meteorology-only predictors without management or soil data, a small temporal test set (3 years, 42 observations per crop), and models' strong dependence on Year and Region features that limit operational forecasting utility.
气候变化和日益增加的天气变率对农业生产力构成重大挑战,特别是在温带地区。本研究利用气象数据考察了气候对捷克共和国大麦、油菜籽、黑麦和小麦产量的影响(2016-2024年),并评估了仅根据气象变量进行产量预测的三种机器学习模型(随机森林、梯度增强、支持向量回归)。测试了三种验证策略:随机(70/ 30%)、空间(基于区域)和时间(最近三年)。随机分区产生了最高的准确性,但有数据泄露的风险,因此不建议用于预测。空间划分显示出低到中等的准确性,这表明ML方法可以在没有数据的地区部分地插值产量(插值),特别是大麦和小麦。时间划分导致预测技能的大幅下降,对黑麦和油菜籽的预测不可靠,表明仅凭气象数据不足以进行可靠的产量预测。支持向量回归在所有验证策略中产生一致的负R2值,表明基本的模型-任务不兼容。小麦的准确性最高(随机:72.57±7.19%,空间:53.45±19.31%,时间:25.10%),主要受年份的影响(>; 50%的重要性)。不考虑年份,空间R2下降到0.2 - 27%,证实了气象变量单独的预测能力有限。作物气候分析显示出不同的响应:黑麦对温度高度敏感,小麦和油菜籽受益于季末的温暖,休眠期间土壤水分过多会降低油菜籽和小麦的产量,而大麦对稳定的温暖条件反应积极。这些发现强调了根据预期任务(插值或预测)选择适当的产率预测验证方法的重要性,以及特征可解释性的价值。主要的限制包括依赖于没有管理或土壤数据的气象预测器,一个小的时间测试集(3年,每种作物42次观测),以及模型对年份和区域特征的强烈依赖,这限制了业务预测的效用。
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引用次数: 0
Quantifying and monetising externalities in Kenya's green bean value chain: implications for stakeholder and policy actions 量化和货币化肯尼亚绿豆价值链的外部性:对利益相关者和政策行动的影响
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-05 DOI: 10.1016/j.jafr.2026.102639
Valiant O. Odhiambo , Sheryl L. Hendriks , Odirilwe Selomane
Growing international demand for fresh green beans is driving producers in Kenya to expand and intensify crop production for export, creating negative environmental, health and social externalities (hidden costs). However, empirical evidence on the magnitude of these externalities remains limited. Estimating these externalities to reveal their magnitude could encourage stakeholder and policy actions that ensure a more environmentally sustainable, health-protective and socially equitable value chain. This study quantified and monetised negative environmental, health and social externalities in Kenya's green bean value chain. True cost accounting approaches, including life cycle assessment, disability-adjusted life years, the True Price methodology and the value of statistical life years, were used to analyse data from secondary sources. The total hidden costs were estimated at 124.03 million USD (range 115.93–132.20), at least twice the 53.92 million USD market value of green beans and almost three times the export value (42.15 million USD). Environmental externalities accounted for 86.87 million USD (range 79.16–94.65), driven mainly by scarce blue water use and greenhouse gas emissions. Health externalities accounted for 0.97 million USD (range 0.58–1.36), primarily from pesticide exposure. Social externalities (36.20 million USD) reflected a large living income gap among smallholder farming households and the presence of child labour. In conclusion, Kenya's green bean value chain creates substantial negative environmental, health and social externalities. There is a need for stakeholder and policy actions to internalise externalities in the value chain. The findings can guide stakeholders and policymakers in developing and implementing strategies to reduce externalities.
国际上对新鲜四季豆的需求日益增长,促使肯尼亚的生产者扩大和加强作物出口生产,造成负面的环境、健康和社会外部性(隐性成本)。然而,关于这些外部性程度的经验证据仍然有限。估计这些外部性以揭示其规模,可鼓励利益攸关方采取政策行动,确保建立一个环境更可持续、保护健康和社会更公平的价值链。这项研究量化并货币化了肯尼亚绿豆价值链中的负面环境、健康和社会外部性。使用真实成本会计方法,包括生命周期评估、残疾调整生命年、真实价格方法和统计生命年的价值来分析二手来源的数据。总隐性成本估计为1.2403亿美元(1.1593 - 132.20美元),至少是绿豆市场价值(5392万美元)的两倍,几乎是出口额(4215万美元)的三倍。环境外部性为8687万美元(范围79.16-94.65),主要由稀缺的蓝水利用和温室气体排放驱动。健康外部性为97万美元(范围0.58-1.36),主要来自农药暴露。社会外部性(3620万美元)反映了小农家庭之间巨大的生活收入差距和童工的存在。总之,肯尼亚的绿豆价值链造成了大量负面的环境、健康和社会外部性。利益相关者和政策行动需要将价值链中的外部性内部化。研究结果可以指导利益相关者和决策者制定和实施减少外部性的战略。
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引用次数: 0
Time-series UAV multispectral imaging for HLB detection via improved U-Net under dynamic orchard environment 动态果园环境下基于改进U-Net的无人机多光谱时序成像HLB检测
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-06 DOI: 10.1016/j.jafr.2026.102643
Haiyong Weng , Hairong Luo , Leilei Su , Boyu Zhang , Dawei Sun , Liangquan Jia , Dapeng Ye
Citrus Huanglongbing (HLB) is the most devastating disease threatening citrus growth, posing a significant challenge to citrus cultivation due to its difficult cure and high transmissibility. Therefore, rapid detection and then removal of HLB infected trees is essential for orchard management. However, the complex and dynamic orchard environment often blocks HLB detection. Using time series analysis methods for large-scale monitoring of citrus orchards is uncommon. This study proposed a two-stage method for HLB detection utilizing time-series multispectral unmanned aerial vehicle (UAV) remote sensing images. The citrus canopy segmentation was carried out firstly using a proposed Canopy-Net with MIoU of 95.4 %, which minimized background interference due to dynamic orchard conditions caused by weed. To improve labeling efficiency of UAV-based HLB datasets, an auxiliary labeling strategy was proposed for 551 trees which only costed approximately 30 min by combining previous canopy segmentation outputs and plant polymerase chain reaction (PCR) results. Furthermore, RS-HLBNet was established for HLB detection in different seasons. It introduced a 2D-3D parallel convolution block in head to enhance spectral feature extraction. To resolve potential semantic mismatches in the original UNet, a CBDCA block was proposed to integrate multi-level skip connection features. The EUCB block further improved feature representation during upsampling. Finally, an MIoU of 88.5 % was achieved during the asymptomatic period (1st August) and 92.2 % in the symptomatic period (22nd November), respectively. In conclusion, this study demonstrated the capability of a time-series UAV multispectral imaging for the rapid HLB detection across orchard scale under dynamic orchard environment.
柑桔黄龙病(HLB)是危害柑桔生长最严重的病害,因其难治和高传播性,对柑桔栽培构成重大挑战。因此,快速检测和清除感染HLB的树木对果园管理至关重要。然而,复杂动态的果园环境往往阻碍HLB的检测。采用时间序列分析方法对柑橘果园进行大规模监测尚属罕见。提出了一种基于时间序列多光谱无人机(UAV)遥感影像的两阶段HLB检测方法。首先,采用MIoU为95.4%的canopy - net进行柑橘冠层分割,最大限度地减少了杂草对果园动态条件的干扰。为了提高基于无人机的HLB数据集的标记效率,结合先前的冠层分割输出和植物聚合酶链反应(PCR)结果,提出了一种辅助标记策略,该策略仅花费约30分钟。建立了不同季节HLB检测的RS-HLBNet。在头部引入2D-3D并行卷积块,增强光谱特征提取。为了解决原始UNet中潜在的语义不匹配问题,提出了一种CBDCA块,该块集成了多级跳过连接特征。EUCB块进一步改进了上采样过程中的特征表示。最后,无症状期(8月1日)和有症状期(11月22日)的MIoU分别达到88.5%和92.2%。综上所述,本研究验证了时间序列无人机多光谱成像在动态果园环境下跨果园尺度快速检测HLB的能力。
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引用次数: 0
Influence of scientific communications on the acceptance of PFAS alternatives on disposable dinnerware and take-out containers, by information source 按信息来源分列的科学传播对接受一次性餐具和外卖容器上的PFAS替代品的影响
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-03-01 Epub Date: 2026-01-07 DOI: 10.1016/j.jafr.2026.102646
Alicia Rihn , Pralhad Bajgain , Nicole Labbé , Gourav Kamboj
Per- and polyfluoroalkyl substances (PFAS) are “forever chemicals” used as treatments on disposable dinnerware and take-out containers to improve their water and oil/grease resistance. They are currently being phased out of use, and alternative options are in development. The objective of this study is to assess how four different information sources (social media influencer, local news, university, and company) influence consumer acceptance of PFAS and PFAS-free alternative treatments. Each treatment presented identical scientific information about PFAS contamination, regulations, and risk mitigation strategies but was attributed to a different source. An online survey of 835 U S. consumers was conducted in 2024. Analysis of Variance (ANOVA) and pairwise t-tests identified significant differences across information sources. Generally, information from influencers was perceived as easier to understand than information from companies. Information from universities and influencers was perceived as more trustworthy than information from news or companies. Regardless of the information source, plant-based treatments were perceived as the most acceptable, followed by generic PFAS-free alternatives. Exposure to information decreased customer acceptance of PFAS, bio-based, and lignin treatments, while acceptance of PFAS-free alternatives improved acceptance. Company-provided information resulted in the largest decrease in PFAS acceptability (by 20.5 %), however this decline was not significantly different from the decreases observed in other information source treatments. Overall, our findings suggest that even brief, source-labeled communications can meaningfully shift consumer acceptance of PFAS alternatives. Importantly, familiar framings such as “plant-based” and “PFAS-free” resonate more strongly than technical terms like “lignin” and “bio-based,” offering a straightforward strategy for shaping public attitudes and supporting the adoption of safer materials.
全氟烷基和多氟烷基物质(PFAS)是“永久化学品”,用于一次性餐具和外卖容器的处理,以提高其耐水性和耐油性/润滑性。它们目前正在逐步停止使用,替代方案正在开发中。本研究的目的是评估四种不同的信息源(社交媒体影响者、当地新闻、大学和公司)如何影响消费者对PFAS和不含PFAS的替代治疗的接受程度。每种处理都提供了关于PFAS污染、法规和风险缓解策略的相同科学信息,但来源不同。2024年对835名美国消费者进行了一项在线调查。方差分析(ANOVA)和两两t检验确定了信息源之间的显著差异。一般来说,来自有影响力的人的信息被认为比来自公司的信息更容易理解。来自大学和有影响力的人的信息被认为比来自新闻或公司的信息更值得信赖。无论信息来源如何,基于植物的治疗被认为是最可接受的,其次是通用的不含pfas的替代品。曝光信息降低了客户对PFAS、生物基和木质素处理的接受度,而接受不含PFAS的替代品则提高了接受度。公司提供的信息导致PFAS可接受性的最大下降(20.5%),然而这种下降与其他信息源处理中观察到的下降没有显著差异。总的来说,我们的研究结果表明,即使是简短的、有来源标签的通信也能有意地改变消费者对PFAS替代品的接受程度。重要的是,与“木质素”和“生物基”等技术术语相比,“植物基”和“不含pfas”等熟悉的框架更能引起共鸣,为塑造公众态度和支持采用更安全的材料提供了直接的策略。
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引用次数: 0
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Journal of Agriculture and Food Research
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