Journal of Agriculture and Food Research最新文献_第9页

Optimization of aqueous extraction conditions for bioactive compounds and antioxidant properties of overripe banana (musa acuminata) using response surface methodology

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-24 DOI: 10.1016/j.jafr.2025.101775

Yee Vern Ng , Majid Khan Majahar Ali , Wan Rosli Wan Ishak

Overripe bananas, often considered waste, represent a rich source of bioactive compounds with significant antioxidant properties. This study focused on optimizing the extraction process of these compounds using Response Surface Methodology (RSM). Key extraction parameters, including water ratio, temperature, and incubation time, were systematically varied based on a central composite design. Responses evaluated included total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity, and ferric reducing antioxidant power (FRAP). The resulting quadratic models exhibited excellent fit (R² > 0.98), validating their predictive reliability. Optimal extraction conditions were determined as a banana:water ratio of 1:3.2 (w/w), temperature of 51.5 °C, and incubation time of 33.5 min. Predicted values under these conditions were 84.7 mg GAE/100 g for TPC, 69.5 mg CEQ/100 g for TFC, 75.1 % for DPPH, and 25.8 mM TE/g for FRAP. Experimental validation closely matched these predictions, yielding 85.3 mg GAE/100 g for TPC, 68.9 mg CEQ/100 g for TFC, 76.5 % for DPPH, and 26.8 mM TE/g for FRAP. The results highlighted the significant influence of extraction temperature and time on bioactive compound recovery, with moderate conditions ensuring maximal yield while minimizing thermal degradation of sensitive compounds. This study underscores the value of integrating RSM in process optimization for efficient extraction of bioactive compounds. Consequently, the optimum extraction conditions provided the highest phenolic concentration and antioxidant yield. By leveraging overripe bananas, this work supports sustainability and promotes the development of high-value functional ingredients.

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引用次数: 0

Revealing the potential hypoglycaemic, hepatorenal and pancreatic protective effects of sugarcane bagasse extract in fructose/streptozotocin induced diabetic rats

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-24 DOI: 10.1016/j.jafr.2025.101769

Wei Ning , Xiaozhuang Peng , Xiaomin Zhang , Abdul Basit , Chitchamai Ovatlarnporn , Opeyemi Joshua Olatunji

Sugarcane bagasse (SB) is a byproduct from sugarcane processing, and it constitute a huge amount of waste product which can amount to humongous environmental nuisance. Studies have shown that SB contains ample quantities of polysaccharides, fibers, flavonoids and phenolic acids which can be explored for their added values. This study aimed at investigating the antidiabetic, antioxidant and hypolipidemic activities of sugarcane bagasse extract (SBE), as well as its hepatorenal and pancreatic protective effects in fructose/streptozotocin-induced diabetic rats. Following the induction of diabetes, the rats were divided into five groups as follows: groups 1 and 2 (normal and diabetic control rats, respectively, treated with normal saline), groups 3 and 4 received 250 and 500 mg/kg of SBE, respectively, and group 5 received 200 mg/kg of metformin. Assessment of diabetes was performed by evaluating metabolic parameters (body weight, food and water intake), blood glucose level, glucose tolerance, biochemical and oxidative stress parameters as well as histopathological analysis of the pancreas, kidney and liver tissues. The binding affinities of some of the compounds identified by LCMS were also assessed against antioxidant enzymes including SOD, CAT and GSH. SBE displayed tremendous potentials in managing diabetic symptoms by mitigating hyperglycemia, restoring body weight loss, glucose intolerance, biochemical, and histological changes in Fr/STZ-induced diabetic rats. Furthermore, SBE alleviated oxidative stress by increasing the hepatorenal and pancreatic activities of SOD, CAT and GSH, as well as reducing MDA level in Fr/STZ-induced diabetic rats. The results indicated that luteolin-8-C-rhamnosyl-7-O-rhamnoside showed higher number of groups interacting with residues of these markers, which might be responsible for the high binding affinities of the compound. Overall, these findings suggest that SB has therapeutic potentials against diabetes and diabetes induced multi-organ injury, thus expanding the potential benefits and possible valorization of SB for food and pharmaceutical applications.

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引用次数: 0

Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-24 DOI: 10.1016/j.jafr.2025.101768

Roshina Rabail , Abdur Rauf Khalid , Amin Mousavi Khaneghah , Muhammad Faisal Manzoor , Gholamreza Abdi , Rana Muhammad Aadil

Edible oil blending to formulate a health-protective fatty acid combination has opened new doors in food science.

Material and methods

In this study, a 42-day biosafety assessment of a blended oil (BO) made from oils of coconut (CO), flaxseed (FO), extra virgin olive (EVOO), and sunflower (SFO) was performed on healthy rats. Rats were given these oils as 100 % fat replacement in control.

Results

The results of BO in rats encouraged its health protection properties by significantly lowering body weight gain (68.8 ± 10.84), BMI (0.128 ± 0.01), body fat content (−0.41 ± 3.41), total cholesterol (43.00 ± 7.84), triglycerides (90.00 ± 9.00), total lipid contents (176.00 ± 24.39), and LDL (14.00 ± 4.12) while substantially improving HDL, LFTs, RFTs, and hematology when compared to single oils. BO consumption also indicated maximum protection against cardiovascular risk indices, including atherogenic (0.99 ± 0.16) and coronary (3.07 ± 0.21) indexes. Results for ECG also showed a normal cardiac rhythm in cardiac waves.

Conclusion

Its regular consumption could help reduce the onset of obesity and metabolic diseases.

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引用次数: 0

Transethosomes-encapsulated Bouea macrophylla seed extract for anti-acne application: Formulation optimization, antibacterial efficacy, and safety assessment

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-24 DOI: 10.1016/j.jafr.2025.101764

Phattharawadee Jaikham , Pimporn Leelapornpisid , Khajornsak Tragoolpua , Pimpisid Koonyosying , Wei-Chao Lin , Worrapan Poomanee

Bouea macrophylla seed extract (B-SE), an agricultural waste, exerts notable antibacterial, antioxidant, and anti-inflammatory properties. However, its instability and limited permeability have restricted its applications in functional food and cosmetics. This study thus aims to develop transethosomes-encapsulated B-SE (TE-B-SE) using the thin-film hydration method to improve overall performance. Initially, five factors: %co-surfactant, types of co-surfactant, %B-SE, %total surfactant, and %phospholipid were preliminarily screened using the 2⁵⁻¹ Fractional Factorial Design (FFD). The percentage contribution of each factor on particle size and polydispersity index (PDI) was calculated. Subsequently, significant factors were included in Central Composite Design (CCD) to optimize the transethosomes presenting desirable particle size, narrow PDI, and high entrapment efficiency (EE). The optimized formulation was visualized through transmission electron microscopy (TEM) and its anti-Cutibacterium acnes activity was also examined by agar well diffusion. Additionally, drug release and skin permeation studies were conducted along with an examination of its cytotoxicity to keratinocytes. From the FFD, the type of co-surfactant chiefly influenced particle size and PDI, with %contributions of 18.13 % and 47.93 %, respectively. The system containing propylene glycol, phospholipid, and nonionic surfactants exhibited acceptable characteristics. According to the CCD, %B-SE and %total surfactant significantly impacted the particle size, PDI, and EE. The TEM images illustrate the optimized transethosomes containing spherical bilayer particles with a size of 87.17 ± 0.83 nm, a PDI of 0.271 ± 0.007, and an EE of 93.98 ± 0.17 %. Furthermore, the formulation could inhibit C. acnes with a 15.14 ± 0.68 mm-inhibition zone. Our findings also confirm that transethosomes potentially enhance the skin permeability of B-SE, which potentially offer better therapeutic application.

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引用次数: 0

Extracting phospholipids from gum using liquified dimethyl ether versus supercritical fluid carbon dioxide

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-24 DOI: 10.1016/j.jafr.2025.101772

Sudthida Kamchonemenukool , Donporn Wongwaiwech , Tipawan Thongsook , Monthana Weerawatanakorn

Gum discarded from the degumming stage of refining crude rice bran oil is a good source of phospholipids (PLs), which are widely used in the food, feed, pharmaceutical, and cosmetic industries due to their unique properties and health benefits supporting brain function and liver health. We examined the recovery of PLs from gum using chemical pretreatment following low-pressure liquefied dimethyl ether (LDME) extraction. The PLs extracted from gum using supercritical fluid carbon dioxide as a green technology were used as a reference level. The highest PL level obtained with supercritical fluid carbon dioxide extraction was 38 g/100 g at 70 °C and 60 MPa, using 15 % ethanol as a co-solvent. Pretreating gum with ethanol in a 1:5 (w/v) ratio at 60 °C for 1 h following LDME extraction at 60 °C (0.6–0.8 MPa) for 30 min gave the highest PL content at level of 37 g/100 g, which was close to the highest level obtained by supercritical fluid carbon dioxide extraction. The ethanol pretreatment of gum following LDME extraction has potential as an environmentally friendly method of PL extraction.

{"title":"Extracting phospholipids from gum using liquified dimethyl ether versus supercritical fluid carbon dioxide","authors":"Sudthida Kamchonemenukool , Donporn Wongwaiwech , Tipawan Thongsook , Monthana Weerawatanakorn","doi":"10.1016/j.jafr.2025.101772","DOIUrl":"10.1016/j.jafr.2025.101772","url":null,"abstract":"<div><div>Gum discarded from the degumming stage of refining crude rice bran oil is a good source of phospholipids (PLs), which are widely used in the food, feed, pharmaceutical, and cosmetic industries due to their unique properties and health benefits supporting brain function and liver health. We examined the recovery of PLs from gum using chemical pretreatment following low-pressure liquefied dimethyl ether (LDME) extraction. The PLs extracted from gum using supercritical fluid carbon dioxide as a green technology were used as a reference level. The highest PL level obtained with supercritical fluid carbon dioxide extraction was 38 g/100 g at 70 °C and 60 MPa, using 15 % ethanol as a co-solvent. Pretreating gum with ethanol in a 1:5 (w/v) ratio at 60 °C for 1 h following LDME extraction at 60 °C (0.6–0.8 MPa) for 30 min gave the highest PL content at level of 37 g/100 g, which was close to the highest level obtained by supercritical fluid carbon dioxide extraction. The ethanol pretreatment of gum following LDME extraction has potential as an environmentally friendly method of PL extraction.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"20 ","pages":"Article 101772"},"PeriodicalIF":4.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemicals in plant food waste: Innovative insight and implications

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-24 DOI: 10.1016/j.jafr.2025.101779

Ifrah Usman , Saima Sana , Muhammad Afzaal , Ali Imran , Hafiza Madiha Jaffar , Sadia Sukhera , Muniba Munir , Aasma Asghar , Markos Makiso Urugo

The processing and distribution of plant-based foods generate substantial waste, including seeds, husks, stalks, roots, and oilseed meals, which are often discarded or repurposed as animal feed. These by-products, however, are rich in bioactive phytochemicals with immense health-promoting and functional potential. Despite this, their industrial application in food products faces significant challenges, such as poor solubility, chemical instability, and limited bioavailability, which hinder their full utilization. Addressing these challenges is crucial for unlocking the full potential of phytochemicals, which possess bio-functional properties like anticancer, anti-inflammatory, and antimicrobial effects, as well as the ability to enrich food products with natural colors, flavors, and antioxidants. This review highlights the innovative applications of nanotechnology and green extraction methods as transformative solutions for improving the extraction and delivery of these phytochemicals. Nanotechnology-based delivery systems, such as nano-liposomes, nano-emulsions, and nano-biopolymers, offer promising approaches to overcome stability, solubility, and bioavailability issues, enabling the incorporation of phytochemicals into food systems while optimizing sensory attributes and enhancing functional efficacy. Furthermore, green extraction methods, which are in alignment with circular economy principles, promote sustainability by reducing food waste and creating high-value nutraceutical and pharmaceutical products. Looking forward, future research should focus on refining nanoparticle encapsulation techniques, exploring synergistic interactions between bioactive compounds, and scaling up these innovations for industrial applications. Additionally, the increasing demand for natural, plant-based ingredients presents an opportunity for the development of sustainable, phytochemical-enriched functional foods and dietary supplements. By addressing current challenges and fostering multidisciplinary collaboration, the valorization of plant food waste has the potential to revolutionize the food and healthcare industries, contributing to global health and sustainability goals.

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引用次数: 0

Transfer of Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes to microgreens and soil from contaminated seeds

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-23 DOI: 10.1016/j.jafr.2025.101761

Aishwarya Pradeep Rao , Abani K. Pradhan , Jitendra Patel

In recent years, the consumption of novel salad greens such as microgreens has increased tremendously around the globe because of their health and nutritional benefits. These benefits that include antioxidant activity among others have been attributed to the presence of helpful bioactive compounds. However, due to their production methods and conditions, they have a risk profile that bears a few similarities to that of sprouts. The persistence trends of Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes were studied over the growth period of 14 days. Seeds of daikon, mustard, broccoli, and red cabbage microgreens were contaminated with the three pathogens at high (∼5 Log CFU/g) and low (∼3 Log CFU/g) levels and the microgreens and soil were sampled on days 7 and 14. Microbiological analysis of soil and microgreen samples was carried out using spiral-plating on pathogen specific selective agars. There was a distinct increase on day 7 in the populations of all three pathogens in both the edible leafy green shoot portions and the soil of the microgreens. However, the prevalence levels of all three pathogens decreased by day 14. On day 7, there was no significant difference in the persistence of E. coli and Salmonella in all four microgreens. However, the L. monocytogenes populations were significantly higher in red cabbage than in broccoli, mustard and daikon. On day 14, the levels of all three pathogens did not significantly vary in the microgreens or the soil in which the microgreens were cultivated. In general, lower persistence of Salmonella and .L. monocytogenes was found in red cabbage and mustard microgreens on day 14, respectively. Results suggest that foodborne pathogens can be transferred from contaminated seeds to microgreens and persist in edible portions of microgreens at the point of harvest. Stringent quality assurance measures are required in maintaining microbial quality of seeds to prevent contamination and potential food safety risks.

{"title":"Transfer of Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes to microgreens and soil from contaminated seeds","authors":"Aishwarya Pradeep Rao , Abani K. Pradhan , Jitendra Patel","doi":"10.1016/j.jafr.2025.101761","DOIUrl":"10.1016/j.jafr.2025.101761","url":null,"abstract":"<div><div>In recent years, the consumption of novel salad greens such as microgreens has increased tremendously around the globe because of their health and nutritional benefits. These benefits that include antioxidant activity among others have been attributed to the presence of helpful bioactive compounds. However, due to their production methods and conditions, they have a risk profile that bears a few similarities to that of sprouts. The persistence trends of <em>Salmonella enterica, Escherichia coli</em> O157:H7 and <em>Listeria monocytogenes</em> were studied over the growth period of 14 days. Seeds of daikon, mustard, broccoli, and red cabbage microgreens were contaminated with the three pathogens at high (∼5 Log CFU/g) and low (∼3 Log CFU/g) levels and the microgreens and soil were sampled on days 7 and 14. Microbiological analysis of soil and microgreen samples was carried out using spiral-plating on pathogen specific selective agars. There was a distinct increase on day 7 in the populations of all three pathogens in both the edible leafy green shoot portions and the soil of the microgreens. However, the prevalence levels of all three pathogens decreased by day 14. On day 7, there was no significant difference in the persistence of <em>E. coli</em> and <em>Salmonella</em> in all four microgreens. However, the <em>L. monocytogenes</em> populations were significantly higher in red cabbage than in broccoli, mustard and daikon. On day 14, the levels of all three pathogens did not significantly vary in the microgreens or the soil in which the microgreens were cultivated. In general, lower persistence of <em>Salmonella</em> and .<em>L.</em> <em>monocytogenes</em> was found in red cabbage and mustard microgreens on day 14, respectively. Results suggest that foodborne pathogens can be transferred from contaminated seeds to microgreens and persist in edible portions of microgreens at the point of harvest. Stringent quality assurance measures are required in maintaining microbial quality of seeds to prevent contamination and potential food safety risks.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101761"},"PeriodicalIF":4.8,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence in agriculture: Advancing crop productivity and sustainability

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-23 DOI: 10.1016/j.jafr.2025.101762

Nazish Aijaz , He Lan , Tausif Raza , Muhammad Yaqub , Rashid Iqbal , Muhammad Salman Pathan

The challenges posed by both climate change and population expansion are unlike anything agriculture has ever seen, and in order to sustain and boost agricultural output, new and creative technology must be used. Artificial intelligence (AI) is one such exponent of change that offers possible solutions in a number of agricultural production fields. Emphasis will be placed on robotic automation, machine learning applications, and the concept of precision farming. This research explores how integration in agriculture has made AI an excellent support for decision processes in crop management, providing real-time monitoring and predictive analytics. Higher agricultural yields and resilience are made possible by genetic advancements and AI in resource optimization. However, due to technological, societal, and legal obstacles, the promise for AI in agriculture has not yet materialized. Against this background, this study requires holistic policy frameworks, education, and stakeholder engagement as countermeasures to such challenges. The future potential applications of AI in agriculture continue to change the sector on behalf of improving global food security and sustainability; this concludes the study. This paper tries to bring to light the critical role that AI is most likely to play in shaping future agricultural practices based on an in-depth analysis of the current state of technology and upcoming opportunities.

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引用次数: 0

Effect of osmotic dehydration on qualitative and nutritional characteristics and kinetics of microwave drying of Iranian Quince slices

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-21 DOI: 10.1016/j.jafr.2025.101749

Narges Layeghinia , Safoora Karimi , Habib Abbasi , Khadije Silavi

Iranian quince is a nutritious fruit with antioxidants that protect against diseases. Due to its tough texture, it’s fresh form is rarely consumed. This study investigates the impact of osmotic dehydration (OD) on the quality of microwave-dried quince slices. The OD pretreatment was performed in a sucrose solution (30, 50, and 70◦Bx) and immersed at times of 10, 20, and 30min. Microwave power is also regarded as an independent variable at three levels of 150, 300, and 400 W. The OD pretreatment not only decreased the drying time by up to 32 % but also resulted in dried slices exhibiting greater antioxidant activity, total phenolic content, and vitamin C compared to fresh samples. For instance vitamin C increasing by 66 % in the control sample dried at 180 W, while OD achieved an impressive 87 % increase. The OD pretreatment decreased the drying time by up to 32 %. The dried slices pretreated with OD had greater antioxidant activity, total phenolic content, and vitamin C than fresh samples. Vitamin C increased by 66 % in the control sample dried at 180 W, while OD achieved an 87 % increase. The OD conditions had no significant effect (P > 0.05) on total phenolic content and antioxidant activity, but a significant effect on vitamin C (P ≤ 0.05). Additionally, Microwave power significantly influenced all color parameters of the samples. The least color change (ΔE) occurred in samples dried at 450 W microwave power, 50°Bx osmotic concentration, and 20 min immersion. Midilli et al.’s model was identified as the most effective for describing the microwave drying process. Besides, a comprehensive comparison of recent studies on the use of OD in fruit drying and the information they provide is presented. Consequently, optimal results were obtained at specific conditions, highlighting the effectiveness of the OD pretreatment in enhancing the microwave drying process of Iranian quince slices.

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引用次数: 0

High Resolution Melting DNA analysis for the traceability of plants and juices of blond and pigmented sweet orange

IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agriculture and Food Research

Pub Date : 2025-02-20 DOI: 10.1016/j.jafr.2025.101755

Sebastiano Seminara , Stefania Bennici , Francesco Scollo , Mario Di Guardo , Pablo Aleza , Stefano La Malfa , Alessandra Gentile , Gaetano Distefano

The economic relevance of sweet orange [Citrus sinensis (L.) Osbeck] raised the interest for the set-up of robust and scalable methods to assess the origin of plants and derived products. Molecular markers, such as single-nucleotide polymorphisms (SNPs), represent a robust tool for the univocal identification of the genetic origin of a specific cultivar (or group of accessions) with relevant application for traceability.

In this work, the whole-genome sequencing of 29 accessions representing the four varietal groups of sweet orange (i.e.: Common, Navel, Acidless and Pigmented) allowed the in silico detection of unique SNPs to discriminate each group (group-specific SNPs) and ten pigmented orange accessions (genotype-specific SNPs).

A subset of the SNPs detected in silico was then validated through a high-resolution melting assay (HRM) on additional 81 genotypes held in three ex-situ collections in Italy and Spain and on several matrices: leaves, hand‐squeezed and commercial fresh-squeezed juices, processed orange juices, and beverages. The HRM assay allowed the identification of 13 group-specific and 30 genotype-specific SNPs showing consistent results across the whole germplasm and the different food matrices tested. The HRM assay proved its efficacy also for the identification of juice blending with other sweet orange varieties (with a detection limit of 5 %). The identified unique SNPs represent a valuable tool to trace the varietal correspondence of plants and fruit-derived products. Their implementation with a scalable and robust HRM assay could be readily employed by growers and the food processing industry to ensure traceability along the chain.

{"title":"High Resolution Melting DNA analysis for the traceability of plants and juices of blond and pigmented sweet orange","authors":"Sebastiano Seminara , Stefania Bennici , Francesco Scollo , Mario Di Guardo , Pablo Aleza , Stefano La Malfa , Alessandra Gentile , Gaetano Distefano","doi":"10.1016/j.jafr.2025.101755","DOIUrl":"10.1016/j.jafr.2025.101755","url":null,"abstract":"<div><div>The economic relevance of sweet orange [<em>Citrus sinensis</em> (L.) Osbeck] raised the interest for the set-up of robust and scalable methods to assess the origin of plants and derived products. Molecular markers, such as single-nucleotide polymorphisms (SNPs), represent a robust tool for the univocal identification of the genetic origin of a specific cultivar (or group of accessions) with relevant application for traceability.</div><div>In this work, the whole-genome sequencing of 29 accessions representing the four varietal groups of sweet orange (i.e.: Common, Navel, Acidless and Pigmented) allowed the <em>in</em> <em>silico</em> detection of unique SNPs to discriminate each group (group-specific SNPs) and ten pigmented orange accessions (genotype-specific SNPs).</div><div>A subset of the SNPs detected <em>in</em> <em>silico</em> was then validated through a high-resolution melting assay (HRM) on additional 81 genotypes held in three <em>ex-situ</em> collections in Italy and Spain and on several matrices: leaves, hand‐squeezed and commercial fresh-squeezed juices, processed orange juices, and beverages. The HRM assay allowed the identification of 13 group-specific and 30 genotype-specific SNPs showing consistent results across the whole germplasm and the different food matrices tested. The HRM assay proved its efficacy also for the identification of juice blending with other sweet orange varieties (with a detection limit of 5 %). The identified unique SNPs represent a valuable tool to trace the varietal correspondence of plants and fruit-derived products. Their implementation with a scalable and robust HRM assay could be readily employed by growers and the food processing industry to ensure traceability along the chain.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"21 ","pages":"Article 101755"},"PeriodicalIF":4.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0