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Reproductive growth stages according to the extended BBCH-scale and physical-chemical changes of yellow pitahaya fruit (Hylocereus megalanthus) 扩展bbch尺度下黄火龙果生殖生长阶段及理化变化
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-19 DOI: 10.1016/j.jafr.2026.102699
Ives Yoplac , River Chávez , Laydy M. Mena , Jorge L. Maicelo-Quintana , Alyssa Hidalgo
The yellow pitahaya (Hylocereus megalanthus), native to Central and South America, has high nutritional and commercial value; however, its reproductive phenology and associated physicochemical changes remain poorly characterized under tropical growing conditions, limiting the establishment of precise management and harvest criteria. The aim of this study was to establish phenological growth codes and stages for its reproductive phase according to the extended BBCH scale (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) and to evaluate the physicochemical changes during fruit growth and maturation. Eighteen secondary growth stages were described, grouped into four main stages: bud development (5), flowering (6), fruit development (7), and maturation (8). Weight, volume, mesocarp and endocarp content and thickness, length, diameter, dry matter, total soluble solids, titratable acidity, and maturity index increased during growth; in contrast, epicarp content and thickness, as well as firmness, decreased. Fruit growth followed a double sigmoid pattern, and physiological maturity was reached approximately 17 weeks after anthesis, representing the most suitable stage for harvest under the studied conditions. The proposed BBCH-based scale and the integrated physicochemical indicators provide a practical framework to support decision-making in crop management, pest, nutrient, pollination, and optimal harvest timing, contributing to improved production efficiency and fruit quality in yellow pitahaya cultivation. The scale could also be applied to define the vegetative and reproductive growth stages of other crops.
原产于中美洲和南美洲的黄色火龙果(Hylocereus megalanthus)具有很高的营养和商业价值;然而,在热带生长条件下,其生殖物候和相关的物理化学变化特征仍然很差,限制了精确管理和收获标准的建立。本研究的目的是根据扩展的BBCH量表(Biologische Bundesanstalt, Bundessortenamt and Chemische Industrie)建立其物候生长编码和繁殖阶段,并评价其果实生长和成熟过程中的理化变化。描述了18个次生生长阶段,分为四个主要阶段:芽发育(5),开花(6),果实发育(7)和成熟(8)。重量、体积、中果皮和内果皮含量、厚度、长度、直径、干物质、总可溶性固形物、可滴定酸度和成熟度指数均随生长而增加;外果皮含量、厚度和硬度均降低。果实生长呈双s型,开花后约17周达到生理成熟,是本研究条件下最适宜的收获期。提出的基于bbch的规模和综合理化指标为黄腰果的作物管理、病虫害、养分、授粉和最佳采收时机的决策提供了实用框架,有助于提高黄腰果的生产效率和果实品质。该尺度也可用于确定其他作物的营养和生殖生长阶段。
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引用次数: 0
Quantitative analysis of fifteen saponins in the roots and leaves of Panax notoginseng under different cultivation models using UPLC–Q-Orbitrap HRMS 采用UPLC-Q-Orbitrap质谱法定量分析不同栽培模式下三七根和叶中15种皂苷的含量
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-19 DOI: 10.1016/j.jafr.2026.102690
Shu He , Songmiao Zhang , Yudong Xing , Tongyu Zang , Xiangfu Xu , Marti Z. Hua , Zhenxing Wang , Liangjuan Zhao , Rui Shi , Shu Wang , Chen Ye , Min Yang , Chao Ji , Xiaonan Lu
Panax notoginseng (Burkill) F.H. Chen is a member of the Araliaceae family and is renowned for its health-promoting properties. In this study, a sensitive and reliable analytical method was established for the quantification of 15 saponins in P. notoginseng. This method exhibited excellent linearity (R2 = 0.9970–0.9998) and low limits of detection (0.12–6.31 μg/L) and quantification (0.54–21.03 μg/L). It was subsequently applied to compare samples from conventional farmland and ecological under-forest cultivation systems. The concentrations of Rb1, Rb3, Rc, and Rd in the leaves, as well as PPD-type saponins in the roots of under-forest plants, were significantly higher than those in farmland-grown counterparts. Rb1 was identified as a characteristic saponin in the leaves of under-forest P. notoginseng, while Rb1 and Rh2 were identified as characteristic saponins in the main roots. These results provide a scientific basis for promoting ecological under-forest cultivation practices and offer a robust analytical approach for quality evaluation of P. notoginseng.
三七(Burkill) F.H. Chen是五加科的一员,以其促进健康的特性而闻名。本研究建立了一种灵敏可靠的三七中15种皂苷含量测定方法。该方法线性良好(R2 = 0.9970 ~ 0.9998),低检出限(0.12 ~ 6.31 μg/L),低定量限(0.54 ~ 21.03 μg/L)。随后将其用于比较传统农田和生态林下栽培系统的样本。林下植物叶片中Rb1、Rb3、Rc和Rd的含量以及根系中ppd型皂苷的含量均显著高于农田植物。经鉴定,林下三七叶片中的特征皂苷为Rb1,主根中的特征皂苷为Rb1和Rh2。研究结果为推广林下生态栽培提供了科学依据,并为三七质量评价提供了可靠的分析方法。
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引用次数: 0
Novel wheat peptide GYP ameliorates Helicobacter pylori infection with AMPK activation 新型小麦肽GYP通过激活AMPK改善幽门螺杆菌感染
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-19 DOI: 10.1016/j.jafr.2026.102697
Yixin Dan, Yalin Lai, Yinghuan Wu, Qinghua He, Yanying Zhao
Helicobacter pylori (H. pylori) is a common microaerobic gram-negative bacteria. It colonizes the stomach of human causing gastritis and then gastric cancer. Currently, H. pylori infection is clinically limited to antibiotic-based therapies. With the widespread use of antibiotics, resistance of H. pylori to antibiotics may be a major reason for treatment failure. Therefore, it is imperative to develop alternative strategies to eradicate H. pylori. In the present study, a peptide GYP was identified from wheat germ protein hydrolysate. The minimum bactericidal concentration of synthesized GYP on 1 × 108 CFU/mL H. pylori was 5 mg/mL, accompanied with cell membrane disruption. Meanwhile, GYP inhibited the urease activity of H. pylori and decreased toxin gene vacuolar cytotoxin A and cytotoxin-associated gene A expression. Furthermore, GYP prevented H. pylori colonization in mouse stomach, and subsequently reduced inflammatory cell infiltration, eliminated gastric vascular congestion and improved gastritis induced by H. pylori. In consistent with this, GYP interfered adhesion of H. pylori on the surface of human gastric mucosal epithelial cells. It further protected gastric mucosal epithelial cells against apoptosis triggered by H. pylori. Therefore, GYP might be a promising anti-H. pylori peptide for the alleviation of H. pylori-induced inflammation and subsequent impairment of gastric mucosa.
幽门螺杆菌(h.p ylori)是一种常见的微氧革兰氏阴性菌。它在人的胃中定植,引起胃炎和胃癌。目前,幽门螺杆菌感染在临床上仅限于基于抗生素的治疗。随着抗生素的广泛使用,幽门螺杆菌对抗生素的耐药性可能是治疗失败的主要原因。因此,制定根除幽门螺旋杆菌的替代策略势在必行。本研究从小麦胚芽蛋白水解物中鉴定出一种肽类GYP。合成的GYP对1 × 108 CFU/mL幽门螺杆菌的最低杀菌浓度为5 mg/mL,并伴有细胞膜破坏。同时,GYP抑制幽门螺杆菌脲酶活性,降低毒素基因空泡细胞毒素A和细胞毒素相关基因A的表达。此外,GYP可阻止幽门螺杆菌在小鼠胃中的定植,从而减少炎症细胞浸润,消除胃血管充血,改善幽门螺杆菌引起的胃炎。与此相一致的是,GYP干扰了幽门螺杆菌在人胃粘膜上皮细胞表面的粘附。进一步保护胃粘膜上皮细胞免受幽门螺杆菌引起的凋亡。因此,GYP可能是一种很有前途的抗h。幽门螺杆菌肽减轻幽门螺杆菌引起的炎症和随后的胃粘膜损伤。
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引用次数: 0
Impact of biochar and inorganic fertilizers rates on yield and yield components of maize at Bako western Ethiopia 生物炭和无机肥料用量对埃塞俄比亚西部巴科玉米产量和产量构成的影响
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-19 DOI: 10.1016/j.jafr.2026.102698
Begizew Golla , Nigussie Dechassa , Wassu Mohammed , Almaz Meseret Gezahegn
Soil acidity is a major constraint to maize production in western Ethiopia, where maize is used as a major staple crop. This problem severely limits yield, threatening food security and farmers’ livelihoods. To manage soil acidity and enhance maize productivity, this study was initiated to evaluate the effect of combined application of biochar and inorganic fertilizers. A two-year field experiment (2023/24 and 2024/25) was conducted at Bako, western Ethiopia, using a randomized complete block design (RCBD) arranged in a factorial with three replications. Treatments included three rates of biochar (0, 5, and 10 t ha−1), nitrogen (0, 46, and 92 kgha−1), and phosphorus (0, 34.5, and 69 kgha−1 P2O5). Major agronomic parameters were measured to assess treatment effects. The results showed that combined application of biochar and inorganic fertilizers significantly increased maize leaf area index (P > 0.01) and grain yield, and significantly (P > 0.05) increased biomass, grain number, and grain weight. The highest grain yield obtained with 5 or 10 t ha−1 biochar combined with 92 kgha−1 N and 69 kgha−1 P2O5. Economically, 5 t ha−1 biochar with the same N and P rates was optimal for acid-prone soils. This treatment increased grain yield by 24.1 % over recommended inorganic fertilizer alone and improved biomass, grain number per ear, and grain weight by 33.9 %, 23.2 %, and 12.2 %, respectively. The combined application reduced soil acidity, improved soil properties, and enhanced maize productivity. As the study covered only two seasons, long-term experiments are required to verify the persistent effects of biochar.
土壤酸度是埃塞俄比亚西部玉米生产的主要制约因素,在那里玉米被用作主要的主食作物。这一问题严重限制了产量,威胁到粮食安全和农民的生计。为了控制土壤酸度,提高玉米产量,本研究评价了生物炭与无机肥料配施的效果。在埃塞俄比亚西部的Bako进行了为期两年的现场试验(2023/24和2024/25),采用随机完全区组设计(RCBD),按三次重复安排。处理包括三种浓度的生物炭(0、5和10 kgha−1)、氮(0、46和92 kgha−1)和磷(0、34.5和69 kgha−1 P2O5)。测量主要农艺参数以评估处理效果。结果表明:生物炭与无机肥配施显著提高了玉米叶面积指数(P > 0.01)和籽粒产量,显著提高了生物量、粒数和粒重(P > 0.05)。5或10 t ha−1生物炭配以92 kgha−1 N和69 kgha−1 P2O5,籽粒产量最高。从经济角度看,施用相同氮磷水平的5 t ha - 1生物炭对酸性土壤最适宜。该处理比单施无机肥料增产24.1%,生物量、穗粒数和粒重分别提高33.9%、23.2%和12.2%。复合施用降低了土壤酸度,改善了土壤性质,提高了玉米产量。由于该研究只覆盖了两个季节,因此需要进行长期实验来验证生物炭的持续影响。
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引用次数: 0
Best management practices for reducing nitrous oxide (N2O) emissions in vegetable production systems 减少蔬菜生产系统中氧化亚氮(N2O)排放的最佳管理实践
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-19 DOI: 10.1016/j.jafr.2026.102703
Lokeshwar Kesamreddy , Somasundaram Eagan , Samuel Mathu Ndungu , Parameswari Ettiyagounder , Winnie Ntinyari , Lukas Pawera

Context

The increasing reliance on synthetic nitrogen (N) fertilizers and intensive soil management in vegetable production systems has led to substantial emissions of nitrogenous gases, including ammonia, nitric oxide, and nitrous oxide (N2O). Among these, N2O is a long-lived greenhouse gas with a high global warming potential (273 times that of CO2), making vegetable production a significant contributor to climate forcing due to high N demand and use, frequent irrigation, and low fertilizer recovery.

Objective

This review aims to synthesize current knowledge on best management practices (BMPs) for mitigating N2O emissions from vegetable production systems, with an emphasis on approaches that enhance N use efficiency while sustaining crop productivity and profitability.

Methods

A comprehensive review of peer-reviewed literature was conducted to evaluate N use patterns, N2O production pathways, emission drivers, and mitigation strategies in vegetable cropping systems. Studies examining practices such as fertilizer management, soil amendments, irrigation, microbial use, precision technologies, and integrated approaches were critically reviewed, and knowledge gaps were identified.

Results

The reviewed evidence indicates that excessive N application, poor synchronization between N supply and crop demand, and intensive irrigation are the primary drivers of elevated N2O emissions in vegetable cultivation. BMPs such as nitrification inhibitors; optimized fertilizer rates, timing, and placement; precision fertigation; negative pressure irrigation; and biochar amendments consistently reduced N2O emissions, often without yield penalties. The effectiveness of these strategies varies with soil type, climate, and crop type.

Conclusions

Mitigating N2O emissions in vegetable production requires shifting from input-intensive practices toward precise, holistic N management that integrates fertilizer, water, and soil management strategies. Single interventions can reduce emissions, but their effectiveness is strongly enhanced when implemented as part of coordinated management efforts.

Implications

Adopting BMPs and integrated N management can substantially reduce the climate footprint of vegetable production while maintaining economic viability. Future research should prioritize system-level assessments, long-term field studies including N2O emissions, and region-specific guidelines to support scalable, climate-smart vegetable production.
蔬菜生产系统越来越依赖合成氮肥和集约化土壤管理,导致大量含氮气体排放,包括氨、一氧化氮和一氧化二氮。其中,N2O是一种寿命较长的温室气体,具有较高的全球变暖潜能值(是CO2的273倍),这使得蔬菜生产成为气候强迫的重要贡献者,因为蔬菜对氮的需求和使用较高,灌溉频繁,肥料回收率低。本综述旨在综合目前关于减少蔬菜生产系统N2O排放的最佳管理实践(BMPs)的知识,重点介绍在保持作物生产力和盈利能力的同时提高氮利用效率的方法。方法对同行评议的文献进行综合评价,以评估蔬菜种植系统的N利用模式、N2O产生途径、排放驱动因素和缓解策略。对肥料管理、土壤改良、灌溉、微生物利用、精密技术和综合方法等实践的研究进行了严格审查,并确定了知识空白。结果过量施氮、氮素供需不同步和密集灌溉是蔬菜栽培N2O排放增加的主要驱动因素。硝化抑制剂等bmp;优化施肥量、时间和位置;精密灌溉施肥;负压灌溉;和生物炭的修正持续减少了N2O的排放,通常不会对产量造成惩罚。这些策略的有效性因土壤类型、气候和作物类型而异。结论:减少蔬菜生产中N2O的排放需要从投入密集型的做法转向精确、全面的氮管理,将肥料、水和土壤管理策略结合起来。单一干预措施可以减少排放,但如果作为协调管理工作的一部分加以实施,其有效性将大大提高。采用bmp和氮素综合管理可以显著减少蔬菜生产的气候足迹,同时保持经济可行性。未来的研究应优先考虑系统级评估、包括一氧化二氮排放在内的长期实地研究以及支持可扩展的气候智能型蔬菜生产的区域特定指南。
{"title":"Best management practices for reducing nitrous oxide (N2O) emissions in vegetable production systems","authors":"Lokeshwar Kesamreddy ,&nbsp;Somasundaram Eagan ,&nbsp;Samuel Mathu Ndungu ,&nbsp;Parameswari Ettiyagounder ,&nbsp;Winnie Ntinyari ,&nbsp;Lukas Pawera","doi":"10.1016/j.jafr.2026.102703","DOIUrl":"10.1016/j.jafr.2026.102703","url":null,"abstract":"<div><h3>Context</h3><div>The increasing reliance on synthetic nitrogen (N) fertilizers and intensive soil management in vegetable production systems has led to substantial emissions of nitrogenous gases, including ammonia, nitric oxide, and nitrous oxide (N<sub>2</sub>O). Among these, N<sub>2</sub>O is a long-lived greenhouse gas with a high global warming potential (273 times that of CO<sub>2</sub>), making vegetable production a significant contributor to climate forcing due to high N demand and use, frequent irrigation, and low fertilizer recovery.</div></div><div><h3>Objective</h3><div>This review aims to synthesize current knowledge on best management practices (BMPs) for mitigating N<sub>2</sub>O emissions from vegetable production systems, with an emphasis on approaches that enhance N use efficiency while sustaining crop productivity and profitability.</div></div><div><h3>Methods</h3><div>A comprehensive review of peer-reviewed literature was conducted to evaluate N use patterns, N<sub>2</sub>O production pathways, emission drivers, and mitigation strategies in vegetable cropping systems. Studies examining practices such as fertilizer management, soil amendments, irrigation, microbial use, precision technologies, and integrated approaches were critically reviewed, and knowledge gaps were identified.</div></div><div><h3>Results</h3><div>The reviewed evidence indicates that excessive N application, poor synchronization between N supply and crop demand, and intensive irrigation are the primary drivers of elevated N<sub>2</sub>O emissions in vegetable cultivation. BMPs such as nitrification inhibitors; optimized fertilizer rates, timing, and placement; precision fertigation; negative pressure irrigation; and biochar amendments consistently reduced N<sub>2</sub>O emissions, often without yield penalties. The effectiveness of these strategies varies with soil type, climate, and crop type.</div></div><div><h3>Conclusions</h3><div>Mitigating N<sub>2</sub>O emissions in vegetable production requires shifting from input-intensive practices toward precise, holistic N management that integrates fertilizer, water, and soil management strategies. Single interventions can reduce emissions, but their effectiveness is strongly enhanced when implemented as part of coordinated management efforts.</div></div><div><h3>Implications</h3><div>Adopting BMPs and integrated N management can substantially reduce the climate footprint of vegetable production while maintaining economic viability. Future research should prioritize system-level assessments, long-term field studies including N<sub>2</sub>O emissions, and region-specific guidelines to support scalable, climate-smart vegetable production.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102703"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of antimicrobial food packaging films based on electrospun polycaprolactone nanofibers encapsulating thyme oil nanoemulsions 静电纺聚己内酯纳米纤维包封百里香油纳米乳抗菌食品包装膜的研制
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-19 DOI: 10.1016/j.jafr.2026.102704
Mahfuzur Rahman , Abdus Sobhan , Hari Kotturi , Sarder Sadique , Omer Sadak , Morshed Khandaker
This study developed and characterized a novel antimicrobial food packaging film fabricated from electrospun polycaprolactone (PCL) nanofibers incorporated with thyme oil nanoemulsions (TONEs). The concentration of TONEs in the film ranged from 2.5 to 15 % (v/v). The physicochemical properties, including pH, viscosity, and water absorption of the film were evaluated, with results showing favorable values (TONEs pH ≈ 5.46, viscosity ≈ 0.26 cP, contact angle ≈ 109-1270 and water absorption ≈ 21.47 %). The microstructure of the film was observed using scanning electron microscopy (SEM), which revealed the uniform dispersion of TONEs within the PCL nanofibrous matrix, with average fiber diameters ranging from 555.68 to 571.09 nm. The TONEs interaction within the PCL matrix in the film was evaluated by Fourier-transform infrared (FTIR) spectroscopy, which revealed hydrogen-bonding interactions between thyme oil components and PCL ester groups. The antimicrobial activity of the films was assessed against common foodborne pathogens, including Escherichia coli, Salmonella enterica, Staphylococcus aureus, and Bacillus cereus. The films containing 10 % and 15 % TONEs demonstrated significant inhibition zones on agar plates (up to 25 mm for Salmonella enterica), confirming their excellent antimicrobial efficacy, with minimum inhibitory concentrations (MICs) as low as 0.078 % (v/v) for most pathogens. The nanoemulsion formulation exhibited optimal droplet sizes (75–99 nm) and polydispersity indices (<0.7), ensuring stable encapsulation and controlled release properties. These findings indicate that TONE-loaded PCL nanofibers are promising antimicrobial packaging materials capable of preventing the growth of foodborne pathogens.
本研究以静电纺聚己内酯(PCL)纳米纤维与百里香油纳米乳剂(TONEs)为原料制备了一种新型抗菌食品包装膜。tone在膜中的浓度为2.5% ~ 15% (v/v)。对膜的理化性质,包括pH、粘度和吸水率进行了评价,结果表明,膜的pH≈5.46,粘度≈0.26 cP,接触角≈109-1270,吸水率≈21.47%。利用扫描电镜(SEM)观察了薄膜的微观结构,发现tone在PCL纳米纤维基体内均匀分散,平均纤维直径在555.68 ~ 571.09 nm之间。利用傅里叶变换红外光谱(FTIR)分析了薄膜中PCL基质内的tone相互作用,发现百里香油组分与PCL酯基之间存在氢键相互作用。对常见的食源性致病菌,包括大肠杆菌、肠炎沙门氏菌、金黄色葡萄球菌和蜡样芽孢杆菌进行了抗菌活性评估。含有10%和15% tone的膜在琼脂平板上显示出显著的抑菌区(对肠沙门氏菌的抑菌区可达25 mm),证实了其良好的抑菌效果,对大多数病原体的最低抑菌浓度(mic)低至0.078% (v/v)。该配方具有最佳的微滴尺寸(75 ~ 99 nm)和多分散性指数(<0.7),保证了稳定的包封和控释性能。这些发现表明,tone负载PCL纳米纤维是一种很有前途的抗菌包装材料,能够阻止食源性病原体的生长。
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引用次数: 0
Combining biochar, nitrogen fertilizer and no-tillage to reduce greenhouse gas emissions and increase rice yield in rainfed lowland 结合生物炭、氮肥和免耕,减少温室气体排放,提高雨养低地的水稻产量
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-19 DOI: 10.1016/j.jafr.2026.102700
Maduabuchi Paul Iboko , Elliott Ronald Dossou-Yovo , Niaba Témé , Sunday Ewele Obalum , Simeon Diedhiou , Akissi Klamansoni Manuela Stephanie Konan , Christian Brümmer
No-tillage and biochar amendment are widely regarded as effective strategies to mitigate greenhouse gas (GHG) emissions. However, the combined effects of no-tillage, biochar and nitrogen (N) fertilizer application on rice yield and GHG emissions remain scarcely investigated. We conducted a two-year field experiment in central Côte d’Ivoire to assess how biochar and N-fertilizer application under no-tillage influences methane (CH4) and nitrous oxide (N2O) emissions, global warming potential (GWP), GHG intensity (GHGI), and rainfed lowland rice yield. The experiment included three N-fertilizer rates (0, 60, and 120 kg N ha−1) combined with two biochar rates (3, and 6 t ha−1) under no-tillage, alongside two additional treatments; N-only fertilizer (120 kg N ha−1) under no-tillage, and under manual tillage (conventional practice). No-tillage combined with biochar and N-fertilizer increased N2O emissions (9–96 %), but reduced CH4 emissions (13–21 %) and increased rice yields (4–10 %) compared to conventional practice. This trade-off led to significant reductions in GWP (10–20 %) and GHGI (15–18) by no-tillage combined with biochar and N-fertilizer relative to conventional practice. Co-application of 6 t ha−1 biochar with 60 kg N ha−1 under no-tillage produced the highest partial N productivity. Conversely, applying N-fertilizer alone under no-tillage resulted in 43 % higher CH4 emission than conventional practice. Across treatments, CH4 emissions contributed 95 % of the total GWP, and soil moisture emerged as the main driver of CH4 fluxes. These findings suggest that applying biochar and N-fertilizer under no-tillage represents a promising pathway to enhance rainfed lowland rice yield and reduce GHG emissions.
免耕和生物炭改良被广泛认为是减少温室气体排放的有效策略。然而,对免耕、生物炭和氮肥施用对水稻产量和温室气体排放的综合影响研究甚少。我们在Côte科特迪瓦中部开展了一项为期两年的田间试验,以评估免耕条件下生物炭和氮肥施用对甲烷(CH4)和氧化亚氮(N2O)排放、全球变暖潜势(GWP)、温室气体强度(GHGI)和雨养低地水稻产量的影响。试验包括免耕条件下3种氮肥(0、60和120 kg N ha - 1)配2种生物炭(3和6 t N ha - 1),以及2个附加处理;免耕和人工耕作(常规做法)下的氮肥(120 kg N ha - 1)。与常规做法相比,免耕配施生物炭和氮肥增加了N2O排放(9 - 96%),减少了CH4排放(13 - 21%),提高了水稻产量(4 - 10%)。这种权衡导致与传统做法相比,免耕与生物炭和氮肥相结合可显著降低全球升温潜能值(10 - 20%)和温室气体排放指数(15 - 18%)。在免耕条件下,6 t ha - 1生物炭与60 kg N ha - 1配施可产生最高的部分N生产力。相反,免耕条件下单施氮肥的CH4排放量比常规做法高43%。在不同处理中,CH4排放占全球变暖潜能值的95%,土壤水分成为CH4通量的主要驱动因素。这些发现表明,在免耕条件下施用生物炭和氮肥是提高旱作水稻产量和减少温室气体排放的一条有希望的途径。
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引用次数: 0
Soil physicochemical traits and environmental context shape the efficacy of a halophyte-derived plant growth-promoting bacterial biofertilizer 土壤理化性状和环境背景决定了盐生植物生长促进细菌生物肥料的功效
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-17 DOI: 10.1016/j.jafr.2026.102671
Elena Romano-Rodríguez , M. Rocío Martín-Peláez , Pedro Valle-Romero , Susana Redondo-Gómez , Noris J. Flores-Duarte , Alfonso Campuzano , Eloísa Pajuelo , Ignacio D. Rodríguez-Llorente , Enrique Mateos-Naranjo
PGPB-based biofertilizers offer a sustainable and cost-effective alternative for agriculture; however, their widespread adoption remains limited due to gaps in understanding strain adaptability to the physicochemical variability of soils and the reproducibility of results under field conditions. This study evaluated how soil properties and environmental variability modulate the beneficial effects of a halophytic PGPB inoculum, composed of self-compatible, stress-tolerant strains with multiple growth-promoting traits, on crop productivity. To this end, two experimental approaches were designed in which Beta vulgaris plants were subjected to two inoculation treatments (non-inoculated and inoculated) and grown in two soils with contrasting physicochemical properties, both under controlled greenhouse and field conditions, respectively. Results showed that the bacterial inoculum significantly enhanced. B. vulgaris growth, physiological performance, and nutritional balance under controlled greenhouse conditions. However, inoculation efficacy was strongly soil-dependent and did not scale linearly from greenhouse to field. In Soil 1, characterized by a sandy texture, low organic matter content, and limited water and nutrients retention, inoculation increased leaf dry matter content by 55 % and 91 %, respectively. In contrast, plants grown in Soil 2, with finer texture, higher organic matter content, and greater nutrient availability, exhibited a modest increase of 17 % following inoculation. Field validation further confirmed this context dependency. While inoculation enhanced plant yield and physiological performance in plants grown in experimental Area 1, no significant benefits were detected in Area 2. In fertile soils like Area 2, optimal nutrient availability and favorable climatic conditions likely reduce plant dependence on microbial related functions, masking inoculation effects. These findings demonstrate that halophytic PGPB inoculum can substantially improves B. vulgaris growth and physiology performance under suboptimal edaphic and climatic conditions, particularly in nutrient-poor, sandy soils, while its benefits are limited in fertile soils with optimal climate, emphasizing the need to consider soil and environmental context for effective biofertilizer use.
以pgpb为基础的生物肥料为农业提供了可持续和具有成本效益的替代方案;然而,由于对土壤物理化学变化的应变适应性和野外条件下结果的可重复性的理解存在差距,它们的广泛采用仍然受到限制。本研究评估了土壤性质和环境变化如何调节盐生植物PGPB接种物对作物生产力的有益影响,该接种物由具有多种生长促进性状的自相容耐压菌株组成。为此,设计了两种试验方法,分别在温室和田间条件下,对甜菜植株进行两种接种处理(未接种和接种),并在两种理化性质截然不同的土壤中生长。结果表明,细菌接种量显著增加。受控温室条件下芥蓝生长、生理性能及营养平衡。然而,接种效果对土壤有很强的依赖性,并不是从温室到田间的线性比例。土壤1为砂质质地,有机质含量低,水分和养分保留有限,接种后叶片干物质含量分别提高了55%和91%。相比之下,土壤2中生长的植物质地更细,有机质含量更高,养分有效性更高,接种后表现出17%的适度增长。字段验证进一步确认了这个上下文依赖性。接种可提高1区植株的产量和生理性能,但2区无显著效果。在2区这样的肥沃土壤中,最佳的养分有效性和有利的气候条件可能降低了植物对微生物相关功能的依赖,掩盖了接种效应。这些研究结果表明,盐生植物PGPB接种可以在次优的土壤和气候条件下,特别是在营养贫乏的沙质土壤中,显著改善普通白杨的生长和生理性能,而在最佳气候条件下的肥沃土壤中,其益处有限,强调需要考虑土壤和环境背景来有效利用生物肥料。
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引用次数: 0
Sunite sheep tail fat alleviates ulcerative colitis by regulating intestinal flora and metabolites to activate PPAR signaling pathway 苏尼特羊尾脂通过调节肠道菌群和代谢产物激活PPAR信号通路减轻溃疡性结肠炎
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-17 DOI: 10.1016/j.jafr.2026.102693
Jinhua Baoyindugurong , Pufang Liu , Chenchen Feng , Dan Chen , Lixing Wang , Jiletu Huge , Ronglun Hou , Yawen Li , Xinlei Yan
Ulcerative colitis (UC) is a topic of great concern, and there are no effective medications to treat it. Previous studies have shown that dietary adjustment may alleviate UC. In this study, a multiomics analysis was used to improve the utilization rate of sunite sheep tail fat (STF) and its role in alleviating UC. The results showed that STF alleviated colon shortening and weight loss in UC mice; reduced the levels of interleukin (IL)-6 and interferon-γ; increased the levels of IL-10; and regulated the protein and gene expression of claudin-1 and zonula occludens-1. Normalization of the intestinal flora, changes in phenylalanine-related metabolites by Bacteroides_H and the activation of the peroxisome proliferators-activated receptor signaling pathway may be the mechanisms by which UC can be alleviated in mice. Thus, this study promotes sheep byproduct utilization, and STF dietary intervention may offer an alternative for UC treatment.
溃疡性结肠炎(UC)是一个备受关注的话题,没有有效的药物治疗它。先前的研究表明,饮食调整可以减轻UC。本研究通过多组学分析提高苏尼特羊尾脂肪(STF)的利用率及其在缓解UC中的作用。结果表明,STF可减轻UC小鼠结肠缩短和体重减轻;降低白细胞介素(IL)-6和干扰素-γ水平;IL-10水平升高;调节claudin-1和occlenza -1蛋白和基因的表达。肠道菌群的正常化、拟杆菌(Bacteroides_H)对苯丙氨酸相关代谢产物的改变以及过氧化物酶体增殖物激活受体信号通路的激活可能是减轻小鼠UC的机制。因此,本研究促进了羊副产品的利用,STF饮食干预可能为UC治疗提供替代方案。
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引用次数: 0
Machine learning-enhanced Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy for discrimination of green Arabica and Robusta coffee beans 机器学习增强的衰减全反射傅里叶变换红外光谱(ATR-FTIR)用于鉴别绿阿拉比卡和罗布斯塔咖啡豆
IF 6.2 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-17 DOI: 10.1016/j.jafr.2026.102689
Wenmeng He , Guangshuai Hao , Shiqin Wang , Rui Meng , Dan Wang , Ying Wang , Baojun Xu
Arabica and Robusta are the two primary commercial coffee species, and reliable discrimination between their beans is critical for detecting adulteration. This investigation employed Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy as a rapid, non-destructive technique to classify 14 coffee bean samples (10 Arabica, 4 Robusta). Spectral data were preprocessed using Savitzky-Golay (SG) smoothing, Multiplicative Scatter Correction (MSC), and Standard Normal Variate (SNV) transformation. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were then applied for exploratory analysis and classification model development. Key results demonstrated that SG smoothing optimally preserved essential spectral features while reducing noise and enhancing the signal-to-noise ratio. The SG-PCA approach achieved superior classification performance, whereas MSC-PCA and SNV-PCA showed reduced effectiveness. The PCA-LDA algorithm proved highly effective, yielding comparable or better classification than PCA alone. Critically, SG preprocessing significantly enhanced the PCA-LDA model's performance. This work establishes ATR-FTIR spectroscopy combined with machine learning (PCA-LDA) as a robust, specific method for discriminating Arabica and Robusta coffee beans. The approach demonstrates strong potential for rapid, cost-effective coffee authentication in commercial and regulatory settings.
阿拉比卡和罗布斯塔是两种主要的商业咖啡品种,对它们的咖啡豆进行可靠的区分对于检测掺假至关重要。本研究采用衰减全反射傅里叶变换红外光谱(ATR-FTIR)作为一种快速、非破坏性的技术,对14种咖啡豆样品(10种阿拉比卡,4种罗布斯塔)进行了分类。采用Savitzky-Golay (SG)平滑、乘法散射校正(MSC)和标准正态变量(SNV)变换对光谱数据进行预处理。然后应用主成分分析(PCA)和线性判别分析(LDA)进行探索性分析和分类模型开发。关键结果表明,SG平滑在降低噪声和提高信噪比的同时,能较好地保留基本的频谱特征。SG-PCA方法的分类效果较好,而MSC-PCA和SNV-PCA的分类效果较差。PCA- lda算法被证明是非常有效的,其分类效果与单独PCA相当或更好。关键是,SG预处理显著提高了PCA-LDA模型的性能。这项工作建立了ATR-FTIR光谱结合机器学习(PCA-LDA)作为鉴别阿拉比卡和罗布斯塔咖啡豆的一种鲁棒、特定的方法。该方法显示了在商业和监管环境中快速、具有成本效益的咖啡认证的巨大潜力。
{"title":"Machine learning-enhanced Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy for discrimination of green Arabica and Robusta coffee beans","authors":"Wenmeng He ,&nbsp;Guangshuai Hao ,&nbsp;Shiqin Wang ,&nbsp;Rui Meng ,&nbsp;Dan Wang ,&nbsp;Ying Wang ,&nbsp;Baojun Xu","doi":"10.1016/j.jafr.2026.102689","DOIUrl":"10.1016/j.jafr.2026.102689","url":null,"abstract":"<div><div>Arabica and Robusta are the two primary commercial coffee species, and reliable discrimination between their beans is critical for detecting adulteration. This investigation employed Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy as a rapid, non-destructive technique to classify 14 coffee bean samples (10 Arabica, 4 Robusta). Spectral data were preprocessed using Savitzky-Golay (SG) smoothing, Multiplicative Scatter Correction (MSC), and Standard Normal Variate (SNV) transformation. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were then applied for exploratory analysis and classification model development. Key results demonstrated that SG smoothing optimally preserved essential spectral features while reducing noise and enhancing the signal-to-noise ratio. The SG-PCA approach achieved superior classification performance, whereas MSC-PCA and SNV-PCA showed reduced effectiveness. The PCA-LDA algorithm proved highly effective, yielding comparable or better classification than PCA alone. Critically, SG preprocessing significantly enhanced the PCA-LDA model's performance. This work establishes ATR-FTIR spectroscopy combined with machine learning (PCA-LDA) as a robust, specific method for discriminating Arabica and Robusta coffee beans. The approach demonstrates strong potential for rapid, cost-effective coffee authentication in commercial and regulatory settings.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102689"},"PeriodicalIF":6.2,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of Agriculture and Food Research
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