Pub Date : 2026-01-19DOI: 10.1016/j.jafr.2026.102699
Ives Yoplac , River Chávez , Laydy M. Mena , Jorge L. Maicelo-Quintana , Alyssa Hidalgo
The yellow pitahaya (Hylocereus megalanthus), native to Central and South America, has high nutritional and commercial value; however, its reproductive phenology and associated physicochemical changes remain poorly characterized under tropical growing conditions, limiting the establishment of precise management and harvest criteria. The aim of this study was to establish phenological growth codes and stages for its reproductive phase according to the extended BBCH scale (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) and to evaluate the physicochemical changes during fruit growth and maturation. Eighteen secondary growth stages were described, grouped into four main stages: bud development (5), flowering (6), fruit development (7), and maturation (8). Weight, volume, mesocarp and endocarp content and thickness, length, diameter, dry matter, total soluble solids, titratable acidity, and maturity index increased during growth; in contrast, epicarp content and thickness, as well as firmness, decreased. Fruit growth followed a double sigmoid pattern, and physiological maturity was reached approximately 17 weeks after anthesis, representing the most suitable stage for harvest under the studied conditions. The proposed BBCH-based scale and the integrated physicochemical indicators provide a practical framework to support decision-making in crop management, pest, nutrient, pollination, and optimal harvest timing, contributing to improved production efficiency and fruit quality in yellow pitahaya cultivation. The scale could also be applied to define the vegetative and reproductive growth stages of other crops.
原产于中美洲和南美洲的黄色火龙果(Hylocereus megalanthus)具有很高的营养和商业价值;然而,在热带生长条件下,其生殖物候和相关的物理化学变化特征仍然很差,限制了精确管理和收获标准的建立。本研究的目的是根据扩展的BBCH量表(Biologische Bundesanstalt, Bundessortenamt and Chemische Industrie)建立其物候生长编码和繁殖阶段,并评价其果实生长和成熟过程中的理化变化。描述了18个次生生长阶段,分为四个主要阶段:芽发育(5),开花(6),果实发育(7)和成熟(8)。重量、体积、中果皮和内果皮含量、厚度、长度、直径、干物质、总可溶性固形物、可滴定酸度和成熟度指数均随生长而增加;外果皮含量、厚度和硬度均降低。果实生长呈双s型,开花后约17周达到生理成熟,是本研究条件下最适宜的收获期。提出的基于bbch的规模和综合理化指标为黄腰果的作物管理、病虫害、养分、授粉和最佳采收时机的决策提供了实用框架,有助于提高黄腰果的生产效率和果实品质。该尺度也可用于确定其他作物的营养和生殖生长阶段。
{"title":"Reproductive growth stages according to the extended BBCH-scale and physical-chemical changes of yellow pitahaya fruit (Hylocereus megalanthus)","authors":"Ives Yoplac , River Chávez , Laydy M. Mena , Jorge L. Maicelo-Quintana , Alyssa Hidalgo","doi":"10.1016/j.jafr.2026.102699","DOIUrl":"10.1016/j.jafr.2026.102699","url":null,"abstract":"<div><div>The yellow pitahaya (<em>Hylocereus megalanthus</em>), native to Central and South America, has high nutritional and commercial value; however, its reproductive phenology and associated physicochemical changes remain poorly characterized under tropical growing conditions, limiting the establishment of precise management and harvest criteria. The aim of this study was to establish phenological growth codes and stages for its reproductive phase according to the extended BBCH scale (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) and to evaluate the physicochemical changes during fruit growth and maturation. Eighteen secondary growth stages were described, grouped into four main stages: bud development (5), flowering (6), fruit development (7), and maturation (8). Weight, volume, mesocarp and endocarp content and thickness, length, diameter, dry matter, total soluble solids, titratable acidity, and maturity index increased during growth; in contrast, epicarp content and thickness, as well as firmness, decreased. Fruit growth followed a double sigmoid pattern, and physiological maturity was reached approximately 17 weeks after anthesis, representing the most suitable stage for harvest under the studied conditions. The proposed BBCH-based scale and the integrated physicochemical indicators provide a practical framework to support decision-making in crop management, pest, nutrient, pollination, and optimal harvest timing, contributing to improved production efficiency and fruit quality in yellow pitahaya cultivation. The scale could also be applied to define the vegetative and reproductive growth stages of other crops.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102699"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.jafr.2026.102690
Shu He , Songmiao Zhang , Yudong Xing , Tongyu Zang , Xiangfu Xu , Marti Z. Hua , Zhenxing Wang , Liangjuan Zhao , Rui Shi , Shu Wang , Chen Ye , Min Yang , Chao Ji , Xiaonan Lu
Panax notoginseng (Burkill) F.H. Chen is a member of the Araliaceae family and is renowned for its health-promoting properties. In this study, a sensitive and reliable analytical method was established for the quantification of 15 saponins in P. notoginseng. This method exhibited excellent linearity (R2 = 0.9970–0.9998) and low limits of detection (0.12–6.31 μg/L) and quantification (0.54–21.03 μg/L). It was subsequently applied to compare samples from conventional farmland and ecological under-forest cultivation systems. The concentrations of Rb1, Rb3, Rc, and Rd in the leaves, as well as PPD-type saponins in the roots of under-forest plants, were significantly higher than those in farmland-grown counterparts. Rb1 was identified as a characteristic saponin in the leaves of under-forest P. notoginseng, while Rb1 and Rh2 were identified as characteristic saponins in the main roots. These results provide a scientific basis for promoting ecological under-forest cultivation practices and offer a robust analytical approach for quality evaluation of P. notoginseng.
{"title":"Quantitative analysis of fifteen saponins in the roots and leaves of Panax notoginseng under different cultivation models using UPLC–Q-Orbitrap HRMS","authors":"Shu He , Songmiao Zhang , Yudong Xing , Tongyu Zang , Xiangfu Xu , Marti Z. Hua , Zhenxing Wang , Liangjuan Zhao , Rui Shi , Shu Wang , Chen Ye , Min Yang , Chao Ji , Xiaonan Lu","doi":"10.1016/j.jafr.2026.102690","DOIUrl":"10.1016/j.jafr.2026.102690","url":null,"abstract":"<div><div><em>Panax notoginseng</em> (Burkill) F.H. Chen is a member of the Araliaceae family and is renowned for its health-promoting properties. In this study, a sensitive and reliable analytical method was established for the quantification of 15 saponins in <em>P. notoginseng</em>. This method exhibited excellent linearity (R<sup>2</sup> = 0.9970–0.9998) and low limits of detection (0.12–6.31 μg/L) and quantification (0.54–21.03 μg/L). It was subsequently applied to compare samples from conventional farmland and ecological under-forest cultivation systems. The concentrations of Rb1, Rb3, Rc, and Rd in the leaves, as well as PPD-type saponins in the roots of under-forest plants, were significantly higher than those in farmland-grown counterparts. Rb1 was identified as a characteristic saponin in the leaves of under-forest <em>P. notoginseng</em>, while Rb1 and Rh2 were identified as characteristic saponins in the main roots. These results provide a scientific basis for promoting ecological under-forest cultivation practices and offer a robust analytical approach for quality evaluation of <em>P. notoginseng</em>.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102690"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.jafr.2026.102697
Yixin Dan, Yalin Lai, Yinghuan Wu, Qinghua He, Yanying Zhao
Helicobacter pylori (H. pylori) is a common microaerobic gram-negative bacteria. It colonizes the stomach of human causing gastritis and then gastric cancer. Currently, H. pylori infection is clinically limited to antibiotic-based therapies. With the widespread use of antibiotics, resistance of H. pylori to antibiotics may be a major reason for treatment failure. Therefore, it is imperative to develop alternative strategies to eradicate H. pylori. In the present study, a peptide GYP was identified from wheat germ protein hydrolysate. The minimum bactericidal concentration of synthesized GYP on 1 × 108 CFU/mL H. pylori was 5 mg/mL, accompanied with cell membrane disruption. Meanwhile, GYP inhibited the urease activity of H. pylori and decreased toxin gene vacuolar cytotoxin A and cytotoxin-associated gene A expression. Furthermore, GYP prevented H. pylori colonization in mouse stomach, and subsequently reduced inflammatory cell infiltration, eliminated gastric vascular congestion and improved gastritis induced by H. pylori. In consistent with this, GYP interfered adhesion of H. pylori on the surface of human gastric mucosal epithelial cells. It further protected gastric mucosal epithelial cells against apoptosis triggered by H. pylori. Therefore, GYP might be a promising anti-H. pylori peptide for the alleviation of H. pylori-induced inflammation and subsequent impairment of gastric mucosa.
{"title":"Novel wheat peptide GYP ameliorates Helicobacter pylori infection with AMPK activation","authors":"Yixin Dan, Yalin Lai, Yinghuan Wu, Qinghua He, Yanying Zhao","doi":"10.1016/j.jafr.2026.102697","DOIUrl":"10.1016/j.jafr.2026.102697","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) is a common microaerobic gram-negative bacteria. It colonizes the stomach of human causing gastritis and then gastric cancer. Currently, <em>H. pylori</em> infection is clinically limited to antibiotic-based therapies. With the widespread use of antibiotics, resistance of <em>H. pylori</em> to antibiotics may be a major reason for treatment failure. Therefore, it is imperative to develop alternative strategies to eradicate <em>H. pylori</em>. In the present study, a peptide GYP was identified from wheat germ protein hydrolysate. The minimum bactericidal concentration of synthesized GYP on 1 × 10<sup>8</sup> CFU/mL <em>H. pylori</em> was 5 mg/mL, accompanied with cell membrane disruption. Meanwhile, GYP inhibited the urease activity of <em>H. pylori</em> and decreased toxin gene <em>vacuolar cytotoxin A</em> and <em>cytotoxin-associated gene A</em> expression. Furthermore, GYP prevented <em>H. pylori</em> colonization in mouse stomach, and subsequently reduced inflammatory cell infiltration, eliminated gastric vascular congestion and improved gastritis induced by <em>H. pylori</em>. In consistent with this, GYP interfered adhesion of <em>H. pylori</em> on the surface of human gastric mucosal epithelial cells. It further protected gastric mucosal epithelial cells against apoptosis triggered by <em>H. pylori</em>. Therefore, GYP might be a promising anti<em>-H. pylori</em> peptide for the alleviation of <em>H. pylori</em>-induced inflammation and subsequent impairment of gastric mucosa.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102697"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil acidity is a major constraint to maize production in western Ethiopia, where maize is used as a major staple crop. This problem severely limits yield, threatening food security and farmers’ livelihoods. To manage soil acidity and enhance maize productivity, this study was initiated to evaluate the effect of combined application of biochar and inorganic fertilizers. A two-year field experiment (2023/24 and 2024/25) was conducted at Bako, western Ethiopia, using a randomized complete block design (RCBD) arranged in a factorial with three replications. Treatments included three rates of biochar (0, 5, and 10 t ha−1), nitrogen (0, 46, and 92 kgha−1), and phosphorus (0, 34.5, and 69 kgha−1 P2O5). Major agronomic parameters were measured to assess treatment effects. The results showed that combined application of biochar and inorganic fertilizers significantly increased maize leaf area index (P > 0.01) and grain yield, and significantly (P > 0.05) increased biomass, grain number, and grain weight. The highest grain yield obtained with 5 or 10 t ha−1 biochar combined with 92 kgha−1 N and 69 kgha−1 P2O5. Economically, 5 t ha−1 biochar with the same N and P rates was optimal for acid-prone soils. This treatment increased grain yield by 24.1 % over recommended inorganic fertilizer alone and improved biomass, grain number per ear, and grain weight by 33.9 %, 23.2 %, and 12.2 %, respectively. The combined application reduced soil acidity, improved soil properties, and enhanced maize productivity. As the study covered only two seasons, long-term experiments are required to verify the persistent effects of biochar.
土壤酸度是埃塞俄比亚西部玉米生产的主要制约因素,在那里玉米被用作主要的主食作物。这一问题严重限制了产量,威胁到粮食安全和农民的生计。为了控制土壤酸度,提高玉米产量,本研究评价了生物炭与无机肥料配施的效果。在埃塞俄比亚西部的Bako进行了为期两年的现场试验(2023/24和2024/25),采用随机完全区组设计(RCBD),按三次重复安排。处理包括三种浓度的生物炭(0、5和10 kgha−1)、氮(0、46和92 kgha−1)和磷(0、34.5和69 kgha−1 P2O5)。测量主要农艺参数以评估处理效果。结果表明:生物炭与无机肥配施显著提高了玉米叶面积指数(P > 0.01)和籽粒产量,显著提高了生物量、粒数和粒重(P > 0.05)。5或10 t ha−1生物炭配以92 kgha−1 N和69 kgha−1 P2O5,籽粒产量最高。从经济角度看,施用相同氮磷水平的5 t ha - 1生物炭对酸性土壤最适宜。该处理比单施无机肥料增产24.1%,生物量、穗粒数和粒重分别提高33.9%、23.2%和12.2%。复合施用降低了土壤酸度,改善了土壤性质,提高了玉米产量。由于该研究只覆盖了两个季节,因此需要进行长期实验来验证生物炭的持续影响。
{"title":"Impact of biochar and inorganic fertilizers rates on yield and yield components of maize at Bako western Ethiopia","authors":"Begizew Golla , Nigussie Dechassa , Wassu Mohammed , Almaz Meseret Gezahegn","doi":"10.1016/j.jafr.2026.102698","DOIUrl":"10.1016/j.jafr.2026.102698","url":null,"abstract":"<div><div>Soil acidity is a major constraint to maize production in western Ethiopia, where maize is used as a major staple crop. This problem severely limits yield, threatening food security and farmers’ livelihoods. To manage soil acidity and enhance maize productivity, this study was initiated to evaluate the effect of combined application of biochar and inorganic fertilizers. A two-year field experiment (2023/24 and 2024/25) was conducted at Bako, western Ethiopia, using a randomized complete block design (RCBD) arranged in a factorial with three replications. Treatments included three rates of biochar (0, 5, and 10 t ha<sup>−1</sup>), nitrogen (0, 46, and 92 kgha<sup>−1</sup>), and phosphorus (0, 34.5, and 69 kgha<sup>−1</sup> P<sub>2</sub>O<sub>5</sub>). Major agronomic parameters were measured to assess treatment effects. The results showed that combined application of biochar and inorganic fertilizers significantly increased maize leaf area index (P > 0.01) and grain yield, and significantly (P > 0.05) increased biomass, grain number, and grain weight. The highest grain yield obtained with 5 or 10 t ha<sup>−1</sup> biochar combined with 92 kgha<sup>−1</sup> N and 69 kgha<sup>−1</sup> P<sub>2</sub>O<sub>5</sub>. Economically, 5 t ha<sup>−1</sup> biochar with the same N and P rates was optimal for acid-prone soils. This treatment increased grain yield by 24.1 % over recommended inorganic fertilizer alone and improved biomass, grain number per ear, and grain weight by 33.9 %, 23.2 %, and 12.2 %, respectively. The combined application reduced soil acidity, improved soil properties, and enhanced maize productivity. As the study covered only two seasons, long-term experiments are required to verify the persistent effects of biochar.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102698"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The increasing reliance on synthetic nitrogen (N) fertilizers and intensive soil management in vegetable production systems has led to substantial emissions of nitrogenous gases, including ammonia, nitric oxide, and nitrous oxide (N2O). Among these, N2O is a long-lived greenhouse gas with a high global warming potential (273 times that of CO2), making vegetable production a significant contributor to climate forcing due to high N demand and use, frequent irrigation, and low fertilizer recovery.
Objective
This review aims to synthesize current knowledge on best management practices (BMPs) for mitigating N2O emissions from vegetable production systems, with an emphasis on approaches that enhance N use efficiency while sustaining crop productivity and profitability.
Methods
A comprehensive review of peer-reviewed literature was conducted to evaluate N use patterns, N2O production pathways, emission drivers, and mitigation strategies in vegetable cropping systems. Studies examining practices such as fertilizer management, soil amendments, irrigation, microbial use, precision technologies, and integrated approaches were critically reviewed, and knowledge gaps were identified.
Results
The reviewed evidence indicates that excessive N application, poor synchronization between N supply and crop demand, and intensive irrigation are the primary drivers of elevated N2O emissions in vegetable cultivation. BMPs such as nitrification inhibitors; optimized fertilizer rates, timing, and placement; precision fertigation; negative pressure irrigation; and biochar amendments consistently reduced N2O emissions, often without yield penalties. The effectiveness of these strategies varies with soil type, climate, and crop type.
Conclusions
Mitigating N2O emissions in vegetable production requires shifting from input-intensive practices toward precise, holistic N management that integrates fertilizer, water, and soil management strategies. Single interventions can reduce emissions, but their effectiveness is strongly enhanced when implemented as part of coordinated management efforts.
Implications
Adopting BMPs and integrated N management can substantially reduce the climate footprint of vegetable production while maintaining economic viability. Future research should prioritize system-level assessments, long-term field studies including N2O emissions, and region-specific guidelines to support scalable, climate-smart vegetable production.
{"title":"Best management practices for reducing nitrous oxide (N2O) emissions in vegetable production systems","authors":"Lokeshwar Kesamreddy , Somasundaram Eagan , Samuel Mathu Ndungu , Parameswari Ettiyagounder , Winnie Ntinyari , Lukas Pawera","doi":"10.1016/j.jafr.2026.102703","DOIUrl":"10.1016/j.jafr.2026.102703","url":null,"abstract":"<div><h3>Context</h3><div>The increasing reliance on synthetic nitrogen (N) fertilizers and intensive soil management in vegetable production systems has led to substantial emissions of nitrogenous gases, including ammonia, nitric oxide, and nitrous oxide (N<sub>2</sub>O). Among these, N<sub>2</sub>O is a long-lived greenhouse gas with a high global warming potential (273 times that of CO<sub>2</sub>), making vegetable production a significant contributor to climate forcing due to high N demand and use, frequent irrigation, and low fertilizer recovery.</div></div><div><h3>Objective</h3><div>This review aims to synthesize current knowledge on best management practices (BMPs) for mitigating N<sub>2</sub>O emissions from vegetable production systems, with an emphasis on approaches that enhance N use efficiency while sustaining crop productivity and profitability.</div></div><div><h3>Methods</h3><div>A comprehensive review of peer-reviewed literature was conducted to evaluate N use patterns, N<sub>2</sub>O production pathways, emission drivers, and mitigation strategies in vegetable cropping systems. Studies examining practices such as fertilizer management, soil amendments, irrigation, microbial use, precision technologies, and integrated approaches were critically reviewed, and knowledge gaps were identified.</div></div><div><h3>Results</h3><div>The reviewed evidence indicates that excessive N application, poor synchronization between N supply and crop demand, and intensive irrigation are the primary drivers of elevated N<sub>2</sub>O emissions in vegetable cultivation. BMPs such as nitrification inhibitors; optimized fertilizer rates, timing, and placement; precision fertigation; negative pressure irrigation; and biochar amendments consistently reduced N<sub>2</sub>O emissions, often without yield penalties. The effectiveness of these strategies varies with soil type, climate, and crop type.</div></div><div><h3>Conclusions</h3><div>Mitigating N<sub>2</sub>O emissions in vegetable production requires shifting from input-intensive practices toward precise, holistic N management that integrates fertilizer, water, and soil management strategies. Single interventions can reduce emissions, but their effectiveness is strongly enhanced when implemented as part of coordinated management efforts.</div></div><div><h3>Implications</h3><div>Adopting BMPs and integrated N management can substantially reduce the climate footprint of vegetable production while maintaining economic viability. Future research should prioritize system-level assessments, long-term field studies including N<sub>2</sub>O emissions, and region-specific guidelines to support scalable, climate-smart vegetable production.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102703"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study developed and characterized a novel antimicrobial food packaging film fabricated from electrospun polycaprolactone (PCL) nanofibers incorporated with thyme oil nanoemulsions (TONEs). The concentration of TONEs in the film ranged from 2.5 to 15 % (v/v). The physicochemical properties, including pH, viscosity, and water absorption of the film were evaluated, with results showing favorable values (TONEs pH ≈ 5.46, viscosity ≈ 0.26 cP, contact angle ≈ 109-1270 and water absorption ≈ 21.47 %). The microstructure of the film was observed using scanning electron microscopy (SEM), which revealed the uniform dispersion of TONEs within the PCL nanofibrous matrix, with average fiber diameters ranging from 555.68 to 571.09 nm. The TONEs interaction within the PCL matrix in the film was evaluated by Fourier-transform infrared (FTIR) spectroscopy, which revealed hydrogen-bonding interactions between thyme oil components and PCL ester groups. The antimicrobial activity of the films was assessed against common foodborne pathogens, including Escherichia coli, Salmonella enterica, Staphylococcus aureus, and Bacillus cereus. The films containing 10 % and 15 % TONEs demonstrated significant inhibition zones on agar plates (up to 25 mm for Salmonella enterica), confirming their excellent antimicrobial efficacy, with minimum inhibitory concentrations (MICs) as low as 0.078 % (v/v) for most pathogens. The nanoemulsion formulation exhibited optimal droplet sizes (75–99 nm) and polydispersity indices (<0.7), ensuring stable encapsulation and controlled release properties. These findings indicate that TONE-loaded PCL nanofibers are promising antimicrobial packaging materials capable of preventing the growth of foodborne pathogens.
{"title":"Development of antimicrobial food packaging films based on electrospun polycaprolactone nanofibers encapsulating thyme oil nanoemulsions","authors":"Mahfuzur Rahman , Abdus Sobhan , Hari Kotturi , Sarder Sadique , Omer Sadak , Morshed Khandaker","doi":"10.1016/j.jafr.2026.102704","DOIUrl":"10.1016/j.jafr.2026.102704","url":null,"abstract":"<div><div>This study developed and characterized a novel antimicrobial food packaging film fabricated from electrospun polycaprolactone (PCL) nanofibers incorporated with thyme oil nanoemulsions (TONEs). The concentration of TONEs in the film ranged from 2.5 to 15 % (v/v). The physicochemical properties, including pH, viscosity, and water absorption of the film were evaluated, with results showing favorable values (TONEs pH ≈ 5.46, viscosity ≈ 0.26 cP, contact angle ≈ 109-127<sup>0</sup> and water absorption ≈ 21.47 %). The microstructure of the film was observed using scanning electron microscopy (SEM), which revealed the uniform dispersion of TONEs within the PCL nanofibrous matrix, with average fiber diameters ranging from 555.68 to 571.09 nm. The TONEs interaction within the PCL matrix in the film was evaluated by Fourier-transform infrared (FTIR) spectroscopy, which revealed hydrogen-bonding interactions between thyme oil components and PCL ester groups. The antimicrobial activity of the films was assessed against common foodborne pathogens, including <em>Escherichia coli</em>, <em>Salmonella enterica</em>, <em>Staphylococcus aureus</em>, and <em>Bacillus cereus</em>. The films containing 10 % and 15 % TONEs demonstrated significant inhibition zones on agar plates (up to 25 mm for <em>Salmonella enterica</em>), confirming their excellent antimicrobial efficacy, with minimum inhibitory concentrations (MICs) as low as 0.078 % (v/v) for most pathogens. The nanoemulsion formulation exhibited optimal droplet sizes (75–99 nm) and polydispersity indices (<0.7), ensuring stable encapsulation and controlled release properties. These findings indicate that TONE-loaded PCL nanofibers are promising antimicrobial packaging materials capable of preventing the growth of foodborne pathogens.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102704"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.jafr.2026.102700
Maduabuchi Paul Iboko , Elliott Ronald Dossou-Yovo , Niaba Témé , Sunday Ewele Obalum , Simeon Diedhiou , Akissi Klamansoni Manuela Stephanie Konan , Christian Brümmer
No-tillage and biochar amendment are widely regarded as effective strategies to mitigate greenhouse gas (GHG) emissions. However, the combined effects of no-tillage, biochar and nitrogen (N) fertilizer application on rice yield and GHG emissions remain scarcely investigated. We conducted a two-year field experiment in central Côte d’Ivoire to assess how biochar and N-fertilizer application under no-tillage influences methane (CH4) and nitrous oxide (N2O) emissions, global warming potential (GWP), GHG intensity (GHGI), and rainfed lowland rice yield. The experiment included three N-fertilizer rates (0, 60, and 120 kg N ha−1) combined with two biochar rates (3, and 6 t ha−1) under no-tillage, alongside two additional treatments; N-only fertilizer (120 kg N ha−1) under no-tillage, and under manual tillage (conventional practice). No-tillage combined with biochar and N-fertilizer increased N2O emissions (9–96 %), but reduced CH4 emissions (13–21 %) and increased rice yields (4–10 %) compared to conventional practice. This trade-off led to significant reductions in GWP (10–20 %) and GHGI (15–18) by no-tillage combined with biochar and N-fertilizer relative to conventional practice. Co-application of 6 t ha−1 biochar with 60 kg N ha−1 under no-tillage produced the highest partial N productivity. Conversely, applying N-fertilizer alone under no-tillage resulted in 43 % higher CH4 emission than conventional practice. Across treatments, CH4 emissions contributed 95 % of the total GWP, and soil moisture emerged as the main driver of CH4 fluxes. These findings suggest that applying biochar and N-fertilizer under no-tillage represents a promising pathway to enhance rainfed lowland rice yield and reduce GHG emissions.
免耕和生物炭改良被广泛认为是减少温室气体排放的有效策略。然而,对免耕、生物炭和氮肥施用对水稻产量和温室气体排放的综合影响研究甚少。我们在Côte科特迪瓦中部开展了一项为期两年的田间试验,以评估免耕条件下生物炭和氮肥施用对甲烷(CH4)和氧化亚氮(N2O)排放、全球变暖潜势(GWP)、温室气体强度(GHGI)和雨养低地水稻产量的影响。试验包括免耕条件下3种氮肥(0、60和120 kg N ha - 1)配2种生物炭(3和6 t N ha - 1),以及2个附加处理;免耕和人工耕作(常规做法)下的氮肥(120 kg N ha - 1)。与常规做法相比,免耕配施生物炭和氮肥增加了N2O排放(9 - 96%),减少了CH4排放(13 - 21%),提高了水稻产量(4 - 10%)。这种权衡导致与传统做法相比,免耕与生物炭和氮肥相结合可显著降低全球升温潜能值(10 - 20%)和温室气体排放指数(15 - 18%)。在免耕条件下,6 t ha - 1生物炭与60 kg N ha - 1配施可产生最高的部分N生产力。相反,免耕条件下单施氮肥的CH4排放量比常规做法高43%。在不同处理中,CH4排放占全球变暖潜能值的95%,土壤水分成为CH4通量的主要驱动因素。这些发现表明,在免耕条件下施用生物炭和氮肥是提高旱作水稻产量和减少温室气体排放的一条有希望的途径。
{"title":"Combining biochar, nitrogen fertilizer and no-tillage to reduce greenhouse gas emissions and increase rice yield in rainfed lowland","authors":"Maduabuchi Paul Iboko , Elliott Ronald Dossou-Yovo , Niaba Témé , Sunday Ewele Obalum , Simeon Diedhiou , Akissi Klamansoni Manuela Stephanie Konan , Christian Brümmer","doi":"10.1016/j.jafr.2026.102700","DOIUrl":"10.1016/j.jafr.2026.102700","url":null,"abstract":"<div><div>No-tillage and biochar amendment are widely regarded as effective strategies to mitigate greenhouse gas (GHG) emissions. However, the combined effects of no-tillage, biochar and nitrogen (N) fertilizer application on rice yield and GHG emissions remain scarcely investigated. We conducted a two-year field experiment in central Côte d’Ivoire to assess how biochar and N-fertilizer application under no-tillage influences methane (CH<sub>4</sub>) and nitrous oxide (N<sub>2</sub>O) emissions, global warming potential (GWP), GHG intensity (GHGI), and rainfed lowland rice yield. The experiment included three N-fertilizer rates (0, 60, and 120 kg N ha<sup>−1</sup>) combined with two biochar rates (3, and 6 t ha<sup>−1</sup>) under no-tillage, alongside two additional treatments; N-only fertilizer (120 kg N ha<sup>−1</sup>) under no-tillage, and under manual tillage (conventional practice). No-tillage combined with biochar and N-fertilizer increased N<sub>2</sub>O emissions (9–96 %), but reduced CH<sub>4</sub> emissions (13–21 %) and increased rice yields (4–10 %) compared to conventional practice. This trade-off led to significant reductions in GWP (10–20 %) and GHGI (15–18) by no-tillage combined with biochar and N-fertilizer relative to conventional practice. Co-application of 6 t ha<sup>−1</sup> biochar with 60 kg N ha<sup>−1</sup> under no-tillage produced the highest partial N productivity. Conversely, applying N-fertilizer alone under no-tillage resulted in 43 % higher CH<sub>4</sub> emission than conventional practice. Across treatments, CH<sub>4</sub> emissions contributed 95 % of the total GWP, and soil moisture emerged as the main driver of CH<sub>4</sub> fluxes. These findings suggest that applying biochar and N-fertilizer under no-tillage represents a promising pathway to enhance rainfed lowland rice yield and reduce GHG emissions.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102700"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-17DOI: 10.1016/j.jafr.2026.102671
Elena Romano-Rodríguez , M. Rocío Martín-Peláez , Pedro Valle-Romero , Susana Redondo-Gómez , Noris J. Flores-Duarte , Alfonso Campuzano , Eloísa Pajuelo , Ignacio D. Rodríguez-Llorente , Enrique Mateos-Naranjo
PGPB-based biofertilizers offer a sustainable and cost-effective alternative for agriculture; however, their widespread adoption remains limited due to gaps in understanding strain adaptability to the physicochemical variability of soils and the reproducibility of results under field conditions. This study evaluated how soil properties and environmental variability modulate the beneficial effects of a halophytic PGPB inoculum, composed of self-compatible, stress-tolerant strains with multiple growth-promoting traits, on crop productivity. To this end, two experimental approaches were designed in which Beta vulgaris plants were subjected to two inoculation treatments (non-inoculated and inoculated) and grown in two soils with contrasting physicochemical properties, both under controlled greenhouse and field conditions, respectively. Results showed that the bacterial inoculum significantly enhanced.B.vulgaris growth, physiological performance, and nutritional balance under controlled greenhouse conditions. However, inoculation efficacy was strongly soil-dependent and did not scale linearly from greenhouse to field. In Soil 1, characterized by a sandy texture, low organic matter content, and limited water and nutrients retention, inoculation increased leaf dry matter content by 55 % and 91 %, respectively. In contrast, plants grown in Soil 2, with finer texture, higher organic matter content, and greater nutrient availability, exhibited a modest increase of 17 % following inoculation. Field validation further confirmed this context dependency. While inoculation enhanced plant yield and physiological performance in plants grown in experimental Area 1, no significant benefits were detected in Area 2. In fertile soils like Area 2, optimal nutrient availability and favorable climatic conditions likely reduce plant dependence on microbial related functions, masking inoculation effects. These findings demonstrate that halophytic PGPB inoculum can substantially improves B. vulgaris growth and physiology performance under suboptimal edaphic and climatic conditions, particularly in nutrient-poor, sandy soils, while its benefits are limited in fertile soils with optimal climate, emphasizing the need to consider soil and environmental context for effective biofertilizer use.
{"title":"Soil physicochemical traits and environmental context shape the efficacy of a halophyte-derived plant growth-promoting bacterial biofertilizer","authors":"Elena Romano-Rodríguez , M. Rocío Martín-Peláez , Pedro Valle-Romero , Susana Redondo-Gómez , Noris J. Flores-Duarte , Alfonso Campuzano , Eloísa Pajuelo , Ignacio D. Rodríguez-Llorente , Enrique Mateos-Naranjo","doi":"10.1016/j.jafr.2026.102671","DOIUrl":"10.1016/j.jafr.2026.102671","url":null,"abstract":"<div><div>PGPB-based biofertilizers offer a sustainable and cost-effective alternative for agriculture; however, their widespread adoption remains limited due to gaps in understanding strain adaptability to the physicochemical variability of soils and the reproducibility of results under field conditions. This study evaluated how soil properties and environmental variability modulate the beneficial effects of a halophytic PGPB inoculum, composed of self-compatible, stress-tolerant strains with multiple growth-promoting traits, on crop productivity. To this end, two experimental approaches were designed in which <em>Beta vulgaris</em> plants were subjected to two inoculation treatments (non-inoculated and inoculated) and grown in two soils with contrasting physicochemical properties, both under controlled greenhouse and field conditions, respectively. Results showed that the bacterial inoculum significantly enhanced<em>.</em> <em>B.</em> <em>vulgaris</em> growth, physiological performance, and nutritional balance under controlled greenhouse conditions. However, inoculation efficacy was strongly soil-dependent and did not scale linearly from greenhouse to field. In Soil 1, characterized by a sandy texture, low organic matter content, and limited water and nutrients retention, inoculation increased leaf dry matter content by 55 % and 91 %, respectively. In contrast, plants grown in Soil 2, with finer texture, higher organic matter content, and greater nutrient availability, exhibited a modest increase of 17 % following inoculation. Field validation further confirmed this context dependency. While inoculation enhanced plant yield and physiological performance in plants grown in experimental Area 1, no significant benefits were detected in Area 2. In fertile soils like Area 2, optimal nutrient availability and favorable climatic conditions likely reduce plant dependence on microbial related functions, masking inoculation effects. These findings demonstrate that halophytic PGPB inoculum can substantially improves <em>B. vulgaris</em> growth and physiology performance under suboptimal edaphic and climatic conditions, particularly in nutrient-poor, sandy soils, while its benefits are limited in fertile soils with optimal climate, emphasizing the need to consider soil and environmental context for effective biofertilizer use.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102671"},"PeriodicalIF":6.2,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-17DOI: 10.1016/j.jafr.2026.102693
Jinhua Baoyindugurong , Pufang Liu , Chenchen Feng , Dan Chen , Lixing Wang , Jiletu Huge , Ronglun Hou , Yawen Li , Xinlei Yan
Ulcerative colitis (UC) is a topic of great concern, and there are no effective medications to treat it. Previous studies have shown that dietary adjustment may alleviate UC. In this study, a multiomics analysis was used to improve the utilization rate of sunite sheep tail fat (STF) and its role in alleviating UC. The results showed that STF alleviated colon shortening and weight loss in UC mice; reduced the levels of interleukin (IL)-6 and interferon-γ; increased the levels of IL-10; and regulated the protein and gene expression of claudin-1 and zonula occludens-1. Normalization of the intestinal flora, changes in phenylalanine-related metabolites by Bacteroides_H and the activation of the peroxisome proliferators-activated receptor signaling pathway may be the mechanisms by which UC can be alleviated in mice. Thus, this study promotes sheep byproduct utilization, and STF dietary intervention may offer an alternative for UC treatment.
{"title":"Sunite sheep tail fat alleviates ulcerative colitis by regulating intestinal flora and metabolites to activate PPAR signaling pathway","authors":"Jinhua Baoyindugurong , Pufang Liu , Chenchen Feng , Dan Chen , Lixing Wang , Jiletu Huge , Ronglun Hou , Yawen Li , Xinlei Yan","doi":"10.1016/j.jafr.2026.102693","DOIUrl":"10.1016/j.jafr.2026.102693","url":null,"abstract":"<div><div>Ulcerative colitis (UC) is a topic of great concern, and there are no effective medications to treat it. Previous studies have shown that dietary adjustment may alleviate UC. In this study, a multiomics analysis was used to improve the utilization rate of sunite sheep tail fat (STF) and its role in alleviating UC. The results showed that STF alleviated colon shortening and weight loss in UC mice; reduced the levels of interleukin (IL)-6 and interferon-γ; increased the levels of IL-10; and regulated the protein and gene expression of claudin-1 and zonula occludens-1. Normalization of the intestinal flora, changes in phenylalanine-related metabolites by <em>Bacteroides_H</em> and the activation of the peroxisome proliferators-activated receptor signaling pathway may be the mechanisms by which UC can be alleviated in mice. Thus, this study promotes sheep byproduct utilization, and STF dietary intervention may offer an alternative for UC treatment.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102693"},"PeriodicalIF":6.2,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-17DOI: 10.1016/j.jafr.2026.102689
Wenmeng He , Guangshuai Hao , Shiqin Wang , Rui Meng , Dan Wang , Ying Wang , Baojun Xu
Arabica and Robusta are the two primary commercial coffee species, and reliable discrimination between their beans is critical for detecting adulteration. This investigation employed Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy as a rapid, non-destructive technique to classify 14 coffee bean samples (10 Arabica, 4 Robusta). Spectral data were preprocessed using Savitzky-Golay (SG) smoothing, Multiplicative Scatter Correction (MSC), and Standard Normal Variate (SNV) transformation. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were then applied for exploratory analysis and classification model development. Key results demonstrated that SG smoothing optimally preserved essential spectral features while reducing noise and enhancing the signal-to-noise ratio. The SG-PCA approach achieved superior classification performance, whereas MSC-PCA and SNV-PCA showed reduced effectiveness. The PCA-LDA algorithm proved highly effective, yielding comparable or better classification than PCA alone. Critically, SG preprocessing significantly enhanced the PCA-LDA model's performance. This work establishes ATR-FTIR spectroscopy combined with machine learning (PCA-LDA) as a robust, specific method for discriminating Arabica and Robusta coffee beans. The approach demonstrates strong potential for rapid, cost-effective coffee authentication in commercial and regulatory settings.
{"title":"Machine learning-enhanced Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy for discrimination of green Arabica and Robusta coffee beans","authors":"Wenmeng He , Guangshuai Hao , Shiqin Wang , Rui Meng , Dan Wang , Ying Wang , Baojun Xu","doi":"10.1016/j.jafr.2026.102689","DOIUrl":"10.1016/j.jafr.2026.102689","url":null,"abstract":"<div><div>Arabica and Robusta are the two primary commercial coffee species, and reliable discrimination between their beans is critical for detecting adulteration. This investigation employed Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy as a rapid, non-destructive technique to classify 14 coffee bean samples (10 Arabica, 4 Robusta). Spectral data were preprocessed using Savitzky-Golay (SG) smoothing, Multiplicative Scatter Correction (MSC), and Standard Normal Variate (SNV) transformation. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were then applied for exploratory analysis and classification model development. Key results demonstrated that SG smoothing optimally preserved essential spectral features while reducing noise and enhancing the signal-to-noise ratio. The SG-PCA approach achieved superior classification performance, whereas MSC-PCA and SNV-PCA showed reduced effectiveness. The PCA-LDA algorithm proved highly effective, yielding comparable or better classification than PCA alone. Critically, SG preprocessing significantly enhanced the PCA-LDA model's performance. This work establishes ATR-FTIR spectroscopy combined with machine learning (PCA-LDA) as a robust, specific method for discriminating Arabica and Robusta coffee beans. The approach demonstrates strong potential for rapid, cost-effective coffee authentication in commercial and regulatory settings.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102689"},"PeriodicalIF":6.2,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}