Tannins originating from root exudates and plant extracts may act as biological nitrification inhibitors (BNIs) for mitigating nitrous oxide (N2O) emissions in the context of circular agriculture. However, the differences of condensed (CT) and hydrolysable (HT) tannins in their effects on soil N2O emissions remain unclear. This study used incubation and pot experiments combined with structural equation modeling (SEM) to evaluate the comprehensive impacts of the proanthocyanidin (PC) and tannic acid (TA) on soil N2O emissions, considering the influence of fertilizer type (urea vs cattle slurry) and plant (absence vs presence). In contrast to the consistent inhibitory effect of 3,4-dimethylpyrazole phosphate (DMPP) applied to both urea- and slurry-amended soils, PC and TA strongly reduced N2O emissions from slurry-amended soil, but had differed impact on N2O (mitigation by PC and enhancement by TA) for urea-amended soil. More importantly, in the presence of cabbage plants, both PC and TA significantly inhibited N2O emissions under both urea and slurry applications, and improved the biomass production and nitrogen use efficiency to different extents. From a microbial perspective, PC and TA likely reduce N2O emissions in urea-amended soil by lowering the (nirS + nirK)/(nosZ I + nosZ II) ratio. In slurry-amended soil, however, they achieve N2O mitigation primarily by increasing the abundance of nosZ II while reducing the abundances of AOA, AOB, nirS, and nirK. In conclusion, the impact of PC and TA on soil N2O emissions can be fertilizer-specific, however in presence of cabbage plants both PC and TA can mitigate N2O emissions regardless fertilizer types. In future it warrants more research to examine the effectiveness of PC and TA on N2O mitigation for different cropping systems under various environmental conditions.
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