Pub Date : 2026-01-21DOI: 10.1016/j.jafr.2026.102707
Linjun Shen , Fangkai Zhao , Junran Li , Lei Yang , Qingyu Feng , Nengliang Yang , Kaifeng Yang , Honglin Liu , Liding Chen
Under long-term greenhouse vegetable production, intensive nutrient inputs enhance crop yields but also lead to the accumulation of antibiotics and heavy metals in greenhouse soils, posing potential risks to crop productivity and soil health. However, studies examining the trade-offs between nutrient inputs and soil contamination remain limited in terms of quantitative assessment of contamination risks. In this study, greenhouse soils with different cultivation histories (<1 year, 5 years, and >10 years) were investigated. The risk quotient (RQ) and a multi-factor heavy metal pollution index (Pt) were used to quantitatively assess the potential risks of soil antibiotics and heavy metals (HMs) contamination to crop growth and soil health, identify key drivers of risks accumulation, and predict contamination risks under different nutrient levels through scenario analyses. The results showed that RQ exhibited “pseudo-persistence” during long-term cultivation and tended to accumulate in lower soil layers, whereas Pt increased continuously with cultivation duration and was predominantly retained in the plow pan layer. Soil nutrients were identified as the key drivers of antibiotic contamination risk, while soil texture played a dominant role in explaining heavy metal contamination risk. Scenario predictions indicated that further increases in nutrient inputs would substantially intensify soil contamination risks; notably, a 10 % increase in soil nutrient levels could elevate antibiotic contamination risk by more than 50 %. This study provides scientific evidence for the coordinated management of nutrient inputs and contamination risks in greenhouse agricultural systems.
{"title":"Impact of intensive greenhouse cultivation on soil health: implications for synergistic management of nutrients and contaminants","authors":"Linjun Shen , Fangkai Zhao , Junran Li , Lei Yang , Qingyu Feng , Nengliang Yang , Kaifeng Yang , Honglin Liu , Liding Chen","doi":"10.1016/j.jafr.2026.102707","DOIUrl":"10.1016/j.jafr.2026.102707","url":null,"abstract":"<div><div>Under long-term greenhouse vegetable production, intensive nutrient inputs enhance crop yields but also lead to the accumulation of antibiotics and heavy metals in greenhouse soils, posing potential risks to crop productivity and soil health. However, studies examining the trade-offs between nutrient inputs and soil contamination remain limited in terms of quantitative assessment of contamination risks. In this study, greenhouse soils with different cultivation histories (<1 year, 5 years, and >10 years) were investigated. The risk quotient (RQ) and a multi-factor heavy metal pollution index (Pt) were used to quantitatively assess the potential risks of soil antibiotics and heavy metals (HMs) contamination to crop growth and soil health, identify key drivers of risks accumulation, and predict contamination risks under different nutrient levels through scenario analyses. The results showed that RQ exhibited “pseudo-persistence” during long-term cultivation and tended to accumulate in lower soil layers, whereas Pt increased continuously with cultivation duration and was predominantly retained in the plow pan layer. Soil nutrients were identified as the key drivers of antibiotic contamination risk, while soil texture played a dominant role in explaining heavy metal contamination risk. Scenario predictions indicated that further increases in nutrient inputs would substantially intensify soil contamination risks; notably, a 10 % increase in soil nutrient levels could elevate antibiotic contamination risk by more than 50 %. This study provides scientific evidence for the coordinated management of nutrient inputs and contamination risks in greenhouse agricultural systems.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102707"},"PeriodicalIF":6.2,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In semi-arid regions, soil erosion and nutrient losses present significant challenges to the sustainability of vegetable farming, as the scarcity of residues limits the adoption of mulching practices. Most crop leftovers are used for animal feed or fuel, which results in limited mulch availability. We evaluate Palash (Butea monosperma) leaf biomass as a mulching material to study crop root-shoot characteristics with hydrological responses, including erosion, nutrient loss, soil properties, and yield in pumpkin (Cucurbita moschata), okra (Abelmoschus esculentus), amaranthus (Amaranthus spp.), and brinjal (Solanum melongena) with mulch (WM) and without mulch (WoM) conditions. Results showed that Palash mulching reduced runoff and soil loss across crops. In brinjal, surface runoff decreased from 66.43 mm (WoM) to 20.88 mm (WM) in 2022 and 154.40 mm–46.26 mm in 2023, while soil loss declined from 2.44 to 0.25 t ha−1 in 2022 and 7.10 to 0.71 t ha−1 in 2023. Okra WM showed lowest nutrient losses (sum of OC, N, P, K) of 3.79 kg ha−1 in 2022 and 8.47 kg ha−1 in 2023, versus highest losses of 17.47 kg ha−1 and 41.19 kg ha−1 under brinjal WoM. Mulch improved soil moisture by 9–36 %. Soil properties improved with mulching, showing lower bulk density (1.67–1.70 vs. 1.70–1.78 g cm−3), higher aggregate stability (70.98–73.25 % vs. 66.93–69.99 %), greater hydraulic conductivity (10.58–11.27 vs. 6.36–7.05 cm h−1), and higher soil organic carbon (0.48–0.58 % vs. 0.38–0.42 %). Mulching increased crop yields by 2.2 % for amaranthus and 40 % for pumpkin. Overall, the results indicate that Palash leaf mulching is an effective practice that reduces soil erosion, conserves soil moisture, and improves crop productivity in semi-arid vegetable production systems.
在半干旱地区,土壤侵蚀和养分流失对蔬菜种植的可持续性提出了重大挑战,因为残留物的稀缺性限制了覆盖物的采用。大多数农作物剩余物被用作动物饲料或燃料,这导致地膜的可用性有限。我们评估了作为地膜材料的巴伐利亚(Butea monosperma)叶片生物量,研究了在有地膜(WM)和无地膜(WoM)条件下,南瓜(Cucurbita moschata)、秋葵(Abelmoschus esculentus)、苋菜(amaranthus spp.)和茄子(Solanum melongena)的根系特征和水文响应,包括侵蚀、养分流失、土壤性质和产量。结果表明,Palash覆盖减少了作物间的径流和土壤流失。在2022年和2023年,地表径流量分别从66.43 mm和154.40 mm减少到20.88 mm和46.26 mm,土壤流失量分别从2.44和7.10分别减少到0.25 t ha - 1和0.71 t ha - 1。秋葵WM的营养损失(OC、N、P、K总和)最低,2022年为3.79 kg ha - 1, 2023年为8.47 kg ha - 1,而茄子WM的损失最高,分别为17.47 kg ha - 1和41.19 kg ha - 1。覆盖使土壤湿度提高了9 - 36%。地膜改善了土壤性质,表现出较低的容重(1.67-1.70比1.70-1.78 g cm−3),较高的团聚体稳定性(70.98 - 73.25%比66.93 - 69.99%),较高的水力导电性(10.58-11.27比6.36-7.05 cm h−1)和较高的土壤有机碳(0.48 - 0.58%比0.38 - 0.42%)。覆盖使苋菜增产2.2%,南瓜增产40%。综上所述,在半干旱蔬菜生产系统中,帕拉什叶覆盖是一种减少土壤侵蚀、保持土壤水分和提高作物生产力的有效措施。
{"title":"Runoff, soil erosion, and crop responses to Palash (Butea monosperma) leaf mulching in semi-arid vegetable production","authors":"Darshan Manikrao Kadam , Dinesh Kumar , Ram Swaroop Yadav , Karuppusamy Rajan , Deepak Singh , Mukesh Meena , Ramkishor Patel , Dinesh Jinger , Kripa Shankar , Deepak Maurya","doi":"10.1016/j.jafr.2026.102702","DOIUrl":"10.1016/j.jafr.2026.102702","url":null,"abstract":"<div><div>In semi-arid regions, soil erosion and nutrient losses present significant challenges to the sustainability of vegetable farming, as the scarcity of residues limits the adoption of mulching practices. Most crop leftovers are used for animal feed or fuel, which results in limited mulch availability. We evaluate Palash (<em>Butea monosperma</em>) leaf biomass as a mulching material to study crop root-shoot characteristics with hydrological responses, including erosion, nutrient loss, soil properties, and yield in pumpkin (<em>Cucurbita moschata</em>), okra (<em>Abelmoschus esculentus</em>), amaranthus (<em>Amaranthus spp.</em>), and brinjal (<em>Solanum melongena</em>) with mulch (WM) and without mulch (WoM) conditions. Results showed that Palash mulching reduced runoff and soil loss across crops. In brinjal, surface runoff decreased from 66.43 mm (WoM) to 20.88 mm (WM) in 2022 and 154.40 mm–46.26 mm in 2023, while soil loss declined from 2.44 to 0.25 t ha<sup>−1</sup> in 2022 and 7.10 to 0.71 t ha<sup>−1</sup> in 2023. Okra WM showed lowest nutrient losses (sum of OC, N, P, K) of 3.79 kg ha<sup>−1</sup> in 2022 and 8.47 kg ha<sup>−1</sup> in 2023, versus highest losses of 17.47 kg ha<sup>−1</sup> and 41.19 kg ha<sup>−1</sup> under brinjal WoM. Mulch improved soil moisture by 9–36 %. Soil properties improved with mulching, showing lower bulk density (1.67–1.70 vs. 1.70–1.78 g cm<sup>−3</sup>), higher aggregate stability (70.98–73.25 % vs. 66.93–69.99 %), greater hydraulic conductivity (10.58–11.27 vs. 6.36–7.05 cm h<sup>−1</sup>), and higher soil organic carbon (0.48–0.58 % vs. 0.38–0.42 %). Mulching increased crop yields by 2.2 % for amaranthus and 40 % for pumpkin. Overall, the results indicate that Palash leaf mulching is an effective practice that reduces soil erosion, conserves soil moisture, and improves crop productivity in semi-arid vegetable production systems.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102702"},"PeriodicalIF":6.2,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-20DOI: 10.1016/j.jafr.2026.102705
Rentao Liao , Wenhua Dongchen , Chun Lin , Zhengjie Liu , Zichao Mao
Crop rotation systems exert a significant influence on soil physicochemical properties and crop growth. However, the mechanisms by which winter oilseed rape coordinates growth and environmental adaptation under paddy-upland rotation systems remain unclear. This study integrated analyses of agronomic traits, yield and quality, transcriptomics, and metabolomics to compare the effects of two consecutive years of either rice-oilseed rape (OsBn) or tobacco-oilseed rape (NtBn) rotations. Results demonstrated that under identical fertilization regimes, the OsBn system was associated with a numerical decreasing trend in yield (259 kg ha−1, approximately 3.67 %) and alterations in seed oil composition (e.g., a significant increase in C15:0 and decrease in C18:3n3) relative to NtBn system. These changes were correlated with diminished soil nitrogen availability and the efficiency of NH4+ uptake. Integrated transcriptomic and metabolomic analyses revealed that the observed trends and alterations in OsBn were likely driven by a pervasive down-regulation of carbon allocation towards sugars and lipids, coupled with an up-regulation of secondary metabolism. This metabolic reprogramming was potentially mediated through the activation of the MAPK signaling cascade, suggesting its role as a central regulator coordinating resource allocation in response to the paddy-upland rotation stress. Consequently, nitrogen management, particularly the timing of topdressing requires particular attention in oilseed rape paddy-upland rotation systems to optimize sustainable practices.
轮作制度对土壤理化性质和作物生长有重要影响。然而,在水旱轮作制度下,冬油菜协调生长和环境适应的机制尚不清楚。本研究综合分析了水稻-油菜(OsBn)和烟草-油菜(NtBn)连续两年轮作的农艺性状、产量和质量、转录组学和代谢组学,比较了水稻-油菜(OsBn)和烟草-油菜(NtBn)的效果。结果表明,在相同施肥制度下,相对于NtBn系统,OsBn系统与产量(259 kg ha - 1,约3.67%)和籽油组成的变化(例如,C15:0显著增加,C18:3n3显著减少)有关。这些变化与土壤氮素有效性和NH4+吸收效率降低有关。综合转录组学和代谢组学分析显示,观察到的OsBn趋势和变化可能是由糖和脂质碳分配普遍下调以及次级代谢上调驱动的。这种代谢重编程可能是通过激活MAPK信号级联介导的,这表明它在水旱轮作胁迫下作为协调资源分配的中心调节器。因此,氮肥管理,特别是追肥的时机需要特别注意油菜水田旱地轮作系统,以优化可持续做法。
{"title":"Integrated transcriptome and metabolome analysis reveals the mechanisms underlying yield trends and alterations in seed oil composition of winter oilseed rape (Brassica napus L.) under paddy-upland rotation systems","authors":"Rentao Liao , Wenhua Dongchen , Chun Lin , Zhengjie Liu , Zichao Mao","doi":"10.1016/j.jafr.2026.102705","DOIUrl":"10.1016/j.jafr.2026.102705","url":null,"abstract":"<div><div>Crop rotation systems exert a significant influence on soil physicochemical properties and crop growth. However, the mechanisms by which winter oilseed rape coordinates growth and environmental adaptation under paddy-upland rotation systems remain unclear. This study integrated analyses of agronomic traits, yield and quality, transcriptomics, and metabolomics to compare the effects of two consecutive years of either rice-oilseed rape (OsBn) or tobacco-oilseed rape (NtBn) rotations. Results demonstrated that under identical fertilization regimes, the OsBn system was associated with a numerical decreasing trend in yield (259 kg ha<sup>−1</sup>, approximately 3.67 %) and alterations in seed oil composition (e.g., a significant increase in C15:0 and decrease in C18:3n3) relative to NtBn system. These changes were correlated with diminished soil nitrogen availability and the efficiency of NH<sub>4</sub><sup>+</sup> uptake. Integrated transcriptomic and metabolomic analyses revealed that the observed trends and alterations in OsBn were likely driven by a pervasive down-regulation of carbon allocation towards sugars and lipids, coupled with an up-regulation of secondary metabolism. This metabolic reprogramming was potentially mediated through the activation of the MAPK signaling cascade, suggesting its role as a central regulator coordinating resource allocation in response to the paddy-upland rotation stress. Consequently, nitrogen management, particularly the timing of topdressing requires particular attention in oilseed rape paddy-upland rotation systems to optimize sustainable practices.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102705"},"PeriodicalIF":6.2,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wheat (Triticum aestivum L.) production in semi-arid regions faces escalating challenges from water scarcity, heat stress, and climatic variability, making improvements in physiological efficiency and yield stability critical for sustaining productivity. This study evaluated the combined influence of spring wheat cultivars and plant growth promoters (PGPs) on crop performance in a semi-arid climate, using a two-year (rabi 2021-22 and 2022-23) split-plot field experiment with three cultivars (DBW-187, K-1006, and K-607) and six PGPs treatments, including nitrobenzene, GA3, seaweed extracts, and micronutrient chelates. Measurements taken at tillering, flowering, and harvest revealed strong cultivar × PGP interactions, with DBW-187 showing superior growth and physiological traits such as higher tiller number (+18.3 %), tiller production rate (+19.7 %), dry matter accumulation (+18.3 %), and improved SPAD, NDVI, and stomatal conductance (+9.5 %). Micronutrient chelates (PGP6) emerged as the most effective promoter, enhancing tiller production (+36.0 % to +50.5 %), dry matter accumulation (+33.4 %), leaf area index (+11.6 %), and pigment concentrations, ultimately contributing to better stress tolerance and higher yield. The DBW-187 + PGP6 combination achieved the highest productivity (5.38 t ha−1), representing a ∼24 % yield increase over the control, while GA3 also produced notable gains (12.8–19.4 %). Overall, the results highlight that integrating nutrient-efficient cultivars with targeted PGPs especially micronutrient formulations and GA3 offers a low-cost, sustainable pathway to enhance physiological resilience and optimize wheat performance in water-limited and heat-stressed agro-ecosystems.
{"title":"Trade-offs and synergies of cultivars and plant growth promoters on wheat physiology and productivity","authors":"Sunil Kumar Prajapati , V.K. Verma , Shivendra Singh , Gurwaan Singh , Sandeep Gawdiya , Shikhar Verma , Naushad Khan , M.Z. Siddiqui , Kuldeep Patel , Deo Narayan Singh , Nilutpal Saikia , Sk Asraful Ali , Sandeep Kumar , Mohammad Hasanain , Vijay Singh Meena , Dinesh Kumar , Shailesh Kumar Singh , Sunita Kumari Meena","doi":"10.1016/j.jafr.2026.102677","DOIUrl":"10.1016/j.jafr.2026.102677","url":null,"abstract":"<div><div>Wheat (<em>Triticum aestivum</em> L.) production in semi-arid regions faces escalating challenges from water scarcity, heat stress, and climatic variability, making improvements in physiological efficiency and yield stability critical for sustaining productivity. This study evaluated the combined influence of spring wheat cultivars and plant growth promoters (PGPs) on crop performance in a semi-arid climate, using a two-year (rabi 2021-22 and 2022-23) split-plot field experiment with three cultivars (DBW-187, K-1006, and K-607) and six PGPs treatments, including nitrobenzene, GA<sub>3</sub>, seaweed extracts, and micronutrient chelates. Measurements taken at tillering, flowering, and harvest revealed strong cultivar × PGP interactions, with DBW-187 showing superior growth and physiological traits such as higher tiller number (+18.3 %), tiller production rate (+19.7 %), dry matter accumulation (+18.3 %), and improved SPAD, NDVI, and stomatal conductance (+9.5 %). Micronutrient chelates (PGP6) emerged as the most effective promoter, enhancing tiller production (+36.0 % to +50.5 %), dry matter accumulation (+33.4 %), leaf area index (+11.6 %), and pigment concentrations, ultimately contributing to better stress tolerance and higher yield. The DBW-187 + PGP<sub>6</sub> combination achieved the highest productivity (5.38 t ha<sup>−1</sup>), representing a ∼24 % yield increase over the control, while GA<sub>3</sub> also produced notable gains (12.8–19.4 %). Overall, the results highlight that integrating nutrient-efficient cultivars with targeted PGPs especially micronutrient formulations and GA<sub>3</sub> offers a low-cost, sustainable pathway to enhance physiological resilience and optimize wheat performance in water-limited and heat-stressed agro-ecosystems.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102677"},"PeriodicalIF":6.2,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study aimed to investigate the differences in metabolite profiles between rice varieties cultivated under organic and conventional conditions and to evaluate their suitability for developing high-quality papads (traditional rice-based snacks). Multivariate statistical analyses (PCA and PLS-DA) revealed distinct metabolic clustering between organic and conventional rice samples. Key differentiating metabolites included myo-inositol, glycerol, sucrose, and linoleic acid in polished rice, and oxalic acid, 5-oxoproline, and glycerol-3-phosphate in bran. Papads made from organic rice exhibited higher protein content, greater levels of bioactive compounds, and significantly enhanced antioxidant activity (p < 0.05) compared to those made from conventional rice. Moreover, organic rice papads demonstrated superior functional properties, such as higher oil uptake and water absorption capacity, along with improved texture, sensory acceptability, and microbial stability, resulting in an extended shelf life. Furthermore, papads made from organic rice exhibited higher functional properties (oil uptake and water absorption), improved texture, sensory acceptability, and microbial stability. Hence, the present study highlights the potential of organic farming to enhance the value of traditional agro-products and suggests the need for further research on the long-term sustainability and functional impacts of organic cultivation practices.
{"title":"Metabolomic profiling, functional, and sensory properties of papads made from two types of rice grown in different conditions","authors":"Kamaldeep Kaur, Sonal Aggarwal, Deepika Kathuria, Narpinder Singh","doi":"10.1016/j.jafr.2026.102644","DOIUrl":"10.1016/j.jafr.2026.102644","url":null,"abstract":"<div><div>The study aimed to investigate the differences in metabolite profiles between rice varieties cultivated under organic and conventional conditions and to evaluate their suitability for developing high-quality <em>papads</em> (traditional rice-based snacks). Multivariate statistical analyses (PCA and PLS-DA) revealed distinct metabolic clustering between organic and conventional rice samples. Key differentiating metabolites included myo-inositol, glycerol, sucrose, and linoleic acid in polished rice, and oxalic acid, 5-oxoproline, and glycerol-3-phosphate in bran. Papads made from organic rice exhibited higher protein content, greater levels of bioactive compounds, and significantly enhanced antioxidant activity (<em>p</em> < 0.05) compared to those made from conventional rice. Moreover, organic rice papads demonstrated superior functional properties, such as higher oil uptake and water absorption capacity, along with improved texture, sensory acceptability, and microbial stability, resulting in an extended shelf life. Furthermore, papads made from organic rice exhibited higher functional properties (oil uptake and water absorption), improved texture, sensory acceptability, and microbial stability. Hence, the present study highlights the potential of organic farming to enhance the value of traditional agro-products and suggests the need for further research on the long-term sustainability and functional impacts of organic cultivation practices.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102644"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.jafr.2026.102699
Ives Yoplac , River Chávez , Laydy M. Mena , Jorge L. Maicelo-Quintana , Alyssa Hidalgo
The yellow pitahaya (Hylocereus megalanthus), native to Central and South America, has high nutritional and commercial value; however, its reproductive phenology and associated physicochemical changes remain poorly characterized under tropical growing conditions, limiting the establishment of precise management and harvest criteria. The aim of this study was to establish phenological growth codes and stages for its reproductive phase according to the extended BBCH scale (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) and to evaluate the physicochemical changes during fruit growth and maturation. Eighteen secondary growth stages were described, grouped into four main stages: bud development (5), flowering (6), fruit development (7), and maturation (8). Weight, volume, mesocarp and endocarp content and thickness, length, diameter, dry matter, total soluble solids, titratable acidity, and maturity index increased during growth; in contrast, epicarp content and thickness, as well as firmness, decreased. Fruit growth followed a double sigmoid pattern, and physiological maturity was reached approximately 17 weeks after anthesis, representing the most suitable stage for harvest under the studied conditions. The proposed BBCH-based scale and the integrated physicochemical indicators provide a practical framework to support decision-making in crop management, pest, nutrient, pollination, and optimal harvest timing, contributing to improved production efficiency and fruit quality in yellow pitahaya cultivation. The scale could also be applied to define the vegetative and reproductive growth stages of other crops.
原产于中美洲和南美洲的黄色火龙果(Hylocereus megalanthus)具有很高的营养和商业价值;然而,在热带生长条件下,其生殖物候和相关的物理化学变化特征仍然很差,限制了精确管理和收获标准的建立。本研究的目的是根据扩展的BBCH量表(Biologische Bundesanstalt, Bundessortenamt and Chemische Industrie)建立其物候生长编码和繁殖阶段,并评价其果实生长和成熟过程中的理化变化。描述了18个次生生长阶段,分为四个主要阶段:芽发育(5),开花(6),果实发育(7)和成熟(8)。重量、体积、中果皮和内果皮含量、厚度、长度、直径、干物质、总可溶性固形物、可滴定酸度和成熟度指数均随生长而增加;外果皮含量、厚度和硬度均降低。果实生长呈双s型,开花后约17周达到生理成熟,是本研究条件下最适宜的收获期。提出的基于bbch的规模和综合理化指标为黄腰果的作物管理、病虫害、养分、授粉和最佳采收时机的决策提供了实用框架,有助于提高黄腰果的生产效率和果实品质。该尺度也可用于确定其他作物的营养和生殖生长阶段。
{"title":"Reproductive growth stages according to the extended BBCH-scale and physical-chemical changes of yellow pitahaya fruit (Hylocereus megalanthus)","authors":"Ives Yoplac , River Chávez , Laydy M. Mena , Jorge L. Maicelo-Quintana , Alyssa Hidalgo","doi":"10.1016/j.jafr.2026.102699","DOIUrl":"10.1016/j.jafr.2026.102699","url":null,"abstract":"<div><div>The yellow pitahaya (<em>Hylocereus megalanthus</em>), native to Central and South America, has high nutritional and commercial value; however, its reproductive phenology and associated physicochemical changes remain poorly characterized under tropical growing conditions, limiting the establishment of precise management and harvest criteria. The aim of this study was to establish phenological growth codes and stages for its reproductive phase according to the extended BBCH scale (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) and to evaluate the physicochemical changes during fruit growth and maturation. Eighteen secondary growth stages were described, grouped into four main stages: bud development (5), flowering (6), fruit development (7), and maturation (8). Weight, volume, mesocarp and endocarp content and thickness, length, diameter, dry matter, total soluble solids, titratable acidity, and maturity index increased during growth; in contrast, epicarp content and thickness, as well as firmness, decreased. Fruit growth followed a double sigmoid pattern, and physiological maturity was reached approximately 17 weeks after anthesis, representing the most suitable stage for harvest under the studied conditions. The proposed BBCH-based scale and the integrated physicochemical indicators provide a practical framework to support decision-making in crop management, pest, nutrient, pollination, and optimal harvest timing, contributing to improved production efficiency and fruit quality in yellow pitahaya cultivation. The scale could also be applied to define the vegetative and reproductive growth stages of other crops.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102699"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.jafr.2026.102690
Shu He , Songmiao Zhang , Yudong Xing , Tongyu Zang , Xiangfu Xu , Marti Z. Hua , Zhenxing Wang , Liangjuan Zhao , Rui Shi , Shu Wang , Chen Ye , Min Yang , Chao Ji , Xiaonan Lu
Panax notoginseng (Burkill) F.H. Chen is a member of the Araliaceae family and is renowned for its health-promoting properties. In this study, a sensitive and reliable analytical method was established for the quantification of 15 saponins in P. notoginseng. This method exhibited excellent linearity (R2 = 0.9970–0.9998) and low limits of detection (0.12–6.31 μg/L) and quantification (0.54–21.03 μg/L). It was subsequently applied to compare samples from conventional farmland and ecological under-forest cultivation systems. The concentrations of Rb1, Rb3, Rc, and Rd in the leaves, as well as PPD-type saponins in the roots of under-forest plants, were significantly higher than those in farmland-grown counterparts. Rb1 was identified as a characteristic saponin in the leaves of under-forest P. notoginseng, while Rb1 and Rh2 were identified as characteristic saponins in the main roots. These results provide a scientific basis for promoting ecological under-forest cultivation practices and offer a robust analytical approach for quality evaluation of P. notoginseng.
{"title":"Quantitative analysis of fifteen saponins in the roots and leaves of Panax notoginseng under different cultivation models using UPLC–Q-Orbitrap HRMS","authors":"Shu He , Songmiao Zhang , Yudong Xing , Tongyu Zang , Xiangfu Xu , Marti Z. Hua , Zhenxing Wang , Liangjuan Zhao , Rui Shi , Shu Wang , Chen Ye , Min Yang , Chao Ji , Xiaonan Lu","doi":"10.1016/j.jafr.2026.102690","DOIUrl":"10.1016/j.jafr.2026.102690","url":null,"abstract":"<div><div><em>Panax notoginseng</em> (Burkill) F.H. Chen is a member of the Araliaceae family and is renowned for its health-promoting properties. In this study, a sensitive and reliable analytical method was established for the quantification of 15 saponins in <em>P. notoginseng</em>. This method exhibited excellent linearity (R<sup>2</sup> = 0.9970–0.9998) and low limits of detection (0.12–6.31 μg/L) and quantification (0.54–21.03 μg/L). It was subsequently applied to compare samples from conventional farmland and ecological under-forest cultivation systems. The concentrations of Rb1, Rb3, Rc, and Rd in the leaves, as well as PPD-type saponins in the roots of under-forest plants, were significantly higher than those in farmland-grown counterparts. Rb1 was identified as a characteristic saponin in the leaves of under-forest <em>P. notoginseng</em>, while Rb1 and Rh2 were identified as characteristic saponins in the main roots. These results provide a scientific basis for promoting ecological under-forest cultivation practices and offer a robust analytical approach for quality evaluation of <em>P. notoginseng</em>.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102690"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.jafr.2026.102697
Yixin Dan, Yalin Lai, Yinghuan Wu, Qinghua He, Yanying Zhao
Helicobacter pylori (H. pylori) is a common microaerobic gram-negative bacteria. It colonizes the stomach of human causing gastritis and then gastric cancer. Currently, H. pylori infection is clinically limited to antibiotic-based therapies. With the widespread use of antibiotics, resistance of H. pylori to antibiotics may be a major reason for treatment failure. Therefore, it is imperative to develop alternative strategies to eradicate H. pylori. In the present study, a peptide GYP was identified from wheat germ protein hydrolysate. The minimum bactericidal concentration of synthesized GYP on 1 × 108 CFU/mL H. pylori was 5 mg/mL, accompanied with cell membrane disruption. Meanwhile, GYP inhibited the urease activity of H. pylori and decreased toxin gene vacuolar cytotoxin A and cytotoxin-associated gene A expression. Furthermore, GYP prevented H. pylori colonization in mouse stomach, and subsequently reduced inflammatory cell infiltration, eliminated gastric vascular congestion and improved gastritis induced by H. pylori. In consistent with this, GYP interfered adhesion of H. pylori on the surface of human gastric mucosal epithelial cells. It further protected gastric mucosal epithelial cells against apoptosis triggered by H. pylori. Therefore, GYP might be a promising anti-H. pylori peptide for the alleviation of H. pylori-induced inflammation and subsequent impairment of gastric mucosa.
{"title":"Novel wheat peptide GYP ameliorates Helicobacter pylori infection with AMPK activation","authors":"Yixin Dan, Yalin Lai, Yinghuan Wu, Qinghua He, Yanying Zhao","doi":"10.1016/j.jafr.2026.102697","DOIUrl":"10.1016/j.jafr.2026.102697","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) is a common microaerobic gram-negative bacteria. It colonizes the stomach of human causing gastritis and then gastric cancer. Currently, <em>H. pylori</em> infection is clinically limited to antibiotic-based therapies. With the widespread use of antibiotics, resistance of <em>H. pylori</em> to antibiotics may be a major reason for treatment failure. Therefore, it is imperative to develop alternative strategies to eradicate <em>H. pylori</em>. In the present study, a peptide GYP was identified from wheat germ protein hydrolysate. The minimum bactericidal concentration of synthesized GYP on 1 × 10<sup>8</sup> CFU/mL <em>H. pylori</em> was 5 mg/mL, accompanied with cell membrane disruption. Meanwhile, GYP inhibited the urease activity of <em>H. pylori</em> and decreased toxin gene <em>vacuolar cytotoxin A</em> and <em>cytotoxin-associated gene A</em> expression. Furthermore, GYP prevented <em>H. pylori</em> colonization in mouse stomach, and subsequently reduced inflammatory cell infiltration, eliminated gastric vascular congestion and improved gastritis induced by <em>H. pylori</em>. In consistent with this, GYP interfered adhesion of <em>H. pylori</em> on the surface of human gastric mucosal epithelial cells. It further protected gastric mucosal epithelial cells against apoptosis triggered by <em>H. pylori</em>. Therefore, GYP might be a promising anti<em>-H. pylori</em> peptide for the alleviation of <em>H. pylori</em>-induced inflammation and subsequent impairment of gastric mucosa.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102697"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil acidity is a major constraint to maize production in western Ethiopia, where maize is used as a major staple crop. This problem severely limits yield, threatening food security and farmers’ livelihoods. To manage soil acidity and enhance maize productivity, this study was initiated to evaluate the effect of combined application of biochar and inorganic fertilizers. A two-year field experiment (2023/24 and 2024/25) was conducted at Bako, western Ethiopia, using a randomized complete block design (RCBD) arranged in a factorial with three replications. Treatments included three rates of biochar (0, 5, and 10 t ha−1), nitrogen (0, 46, and 92 kgha−1), and phosphorus (0, 34.5, and 69 kgha−1 P2O5). Major agronomic parameters were measured to assess treatment effects. The results showed that combined application of biochar and inorganic fertilizers significantly increased maize leaf area index (P > 0.01) and grain yield, and significantly (P > 0.05) increased biomass, grain number, and grain weight. The highest grain yield obtained with 5 or 10 t ha−1 biochar combined with 92 kgha−1 N and 69 kgha−1 P2O5. Economically, 5 t ha−1 biochar with the same N and P rates was optimal for acid-prone soils. This treatment increased grain yield by 24.1 % over recommended inorganic fertilizer alone and improved biomass, grain number per ear, and grain weight by 33.9 %, 23.2 %, and 12.2 %, respectively. The combined application reduced soil acidity, improved soil properties, and enhanced maize productivity. As the study covered only two seasons, long-term experiments are required to verify the persistent effects of biochar.
土壤酸度是埃塞俄比亚西部玉米生产的主要制约因素,在那里玉米被用作主要的主食作物。这一问题严重限制了产量,威胁到粮食安全和农民的生计。为了控制土壤酸度,提高玉米产量,本研究评价了生物炭与无机肥料配施的效果。在埃塞俄比亚西部的Bako进行了为期两年的现场试验(2023/24和2024/25),采用随机完全区组设计(RCBD),按三次重复安排。处理包括三种浓度的生物炭(0、5和10 kgha−1)、氮(0、46和92 kgha−1)和磷(0、34.5和69 kgha−1 P2O5)。测量主要农艺参数以评估处理效果。结果表明:生物炭与无机肥配施显著提高了玉米叶面积指数(P > 0.01)和籽粒产量,显著提高了生物量、粒数和粒重(P > 0.05)。5或10 t ha−1生物炭配以92 kgha−1 N和69 kgha−1 P2O5,籽粒产量最高。从经济角度看,施用相同氮磷水平的5 t ha - 1生物炭对酸性土壤最适宜。该处理比单施无机肥料增产24.1%,生物量、穗粒数和粒重分别提高33.9%、23.2%和12.2%。复合施用降低了土壤酸度,改善了土壤性质,提高了玉米产量。由于该研究只覆盖了两个季节,因此需要进行长期实验来验证生物炭的持续影响。
{"title":"Impact of biochar and inorganic fertilizers rates on yield and yield components of maize at Bako western Ethiopia","authors":"Begizew Golla , Nigussie Dechassa , Wassu Mohammed , Almaz Meseret Gezahegn","doi":"10.1016/j.jafr.2026.102698","DOIUrl":"10.1016/j.jafr.2026.102698","url":null,"abstract":"<div><div>Soil acidity is a major constraint to maize production in western Ethiopia, where maize is used as a major staple crop. This problem severely limits yield, threatening food security and farmers’ livelihoods. To manage soil acidity and enhance maize productivity, this study was initiated to evaluate the effect of combined application of biochar and inorganic fertilizers. A two-year field experiment (2023/24 and 2024/25) was conducted at Bako, western Ethiopia, using a randomized complete block design (RCBD) arranged in a factorial with three replications. Treatments included three rates of biochar (0, 5, and 10 t ha<sup>−1</sup>), nitrogen (0, 46, and 92 kgha<sup>−1</sup>), and phosphorus (0, 34.5, and 69 kgha<sup>−1</sup> P<sub>2</sub>O<sub>5</sub>). Major agronomic parameters were measured to assess treatment effects. The results showed that combined application of biochar and inorganic fertilizers significantly increased maize leaf area index (P > 0.01) and grain yield, and significantly (P > 0.05) increased biomass, grain number, and grain weight. The highest grain yield obtained with 5 or 10 t ha<sup>−1</sup> biochar combined with 92 kgha<sup>−1</sup> N and 69 kgha<sup>−1</sup> P<sub>2</sub>O<sub>5</sub>. Economically, 5 t ha<sup>−1</sup> biochar with the same N and P rates was optimal for acid-prone soils. This treatment increased grain yield by 24.1 % over recommended inorganic fertilizer alone and improved biomass, grain number per ear, and grain weight by 33.9 %, 23.2 %, and 12.2 %, respectively. The combined application reduced soil acidity, improved soil properties, and enhanced maize productivity. As the study covered only two seasons, long-term experiments are required to verify the persistent effects of biochar.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102698"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The increasing reliance on synthetic nitrogen (N) fertilizers and intensive soil management in vegetable production systems has led to substantial emissions of nitrogenous gases, including ammonia, nitric oxide, and nitrous oxide (N2O). Among these, N2O is a long-lived greenhouse gas with a high global warming potential (273 times that of CO2), making vegetable production a significant contributor to climate forcing due to high N demand and use, frequent irrigation, and low fertilizer recovery.
Objective
This review aims to synthesize current knowledge on best management practices (BMPs) for mitigating N2O emissions from vegetable production systems, with an emphasis on approaches that enhance N use efficiency while sustaining crop productivity and profitability.
Methods
A comprehensive review of peer-reviewed literature was conducted to evaluate N use patterns, N2O production pathways, emission drivers, and mitigation strategies in vegetable cropping systems. Studies examining practices such as fertilizer management, soil amendments, irrigation, microbial use, precision technologies, and integrated approaches were critically reviewed, and knowledge gaps were identified.
Results
The reviewed evidence indicates that excessive N application, poor synchronization between N supply and crop demand, and intensive irrigation are the primary drivers of elevated N2O emissions in vegetable cultivation. BMPs such as nitrification inhibitors; optimized fertilizer rates, timing, and placement; precision fertigation; negative pressure irrigation; and biochar amendments consistently reduced N2O emissions, often without yield penalties. The effectiveness of these strategies varies with soil type, climate, and crop type.
Conclusions
Mitigating N2O emissions in vegetable production requires shifting from input-intensive practices toward precise, holistic N management that integrates fertilizer, water, and soil management strategies. Single interventions can reduce emissions, but their effectiveness is strongly enhanced when implemented as part of coordinated management efforts.
Implications
Adopting BMPs and integrated N management can substantially reduce the climate footprint of vegetable production while maintaining economic viability. Future research should prioritize system-level assessments, long-term field studies including N2O emissions, and region-specific guidelines to support scalable, climate-smart vegetable production.
{"title":"Best management practices for reducing nitrous oxide (N2O) emissions in vegetable production systems","authors":"Lokeshwar Kesamreddy , Somasundaram Eagan , Samuel Mathu Ndungu , Parameswari Ettiyagounder , Winnie Ntinyari , Lukas Pawera","doi":"10.1016/j.jafr.2026.102703","DOIUrl":"10.1016/j.jafr.2026.102703","url":null,"abstract":"<div><h3>Context</h3><div>The increasing reliance on synthetic nitrogen (N) fertilizers and intensive soil management in vegetable production systems has led to substantial emissions of nitrogenous gases, including ammonia, nitric oxide, and nitrous oxide (N<sub>2</sub>O). Among these, N<sub>2</sub>O is a long-lived greenhouse gas with a high global warming potential (273 times that of CO<sub>2</sub>), making vegetable production a significant contributor to climate forcing due to high N demand and use, frequent irrigation, and low fertilizer recovery.</div></div><div><h3>Objective</h3><div>This review aims to synthesize current knowledge on best management practices (BMPs) for mitigating N<sub>2</sub>O emissions from vegetable production systems, with an emphasis on approaches that enhance N use efficiency while sustaining crop productivity and profitability.</div></div><div><h3>Methods</h3><div>A comprehensive review of peer-reviewed literature was conducted to evaluate N use patterns, N<sub>2</sub>O production pathways, emission drivers, and mitigation strategies in vegetable cropping systems. Studies examining practices such as fertilizer management, soil amendments, irrigation, microbial use, precision technologies, and integrated approaches were critically reviewed, and knowledge gaps were identified.</div></div><div><h3>Results</h3><div>The reviewed evidence indicates that excessive N application, poor synchronization between N supply and crop demand, and intensive irrigation are the primary drivers of elevated N<sub>2</sub>O emissions in vegetable cultivation. BMPs such as nitrification inhibitors; optimized fertilizer rates, timing, and placement; precision fertigation; negative pressure irrigation; and biochar amendments consistently reduced N<sub>2</sub>O emissions, often without yield penalties. The effectiveness of these strategies varies with soil type, climate, and crop type.</div></div><div><h3>Conclusions</h3><div>Mitigating N<sub>2</sub>O emissions in vegetable production requires shifting from input-intensive practices toward precise, holistic N management that integrates fertilizer, water, and soil management strategies. Single interventions can reduce emissions, but their effectiveness is strongly enhanced when implemented as part of coordinated management efforts.</div></div><div><h3>Implications</h3><div>Adopting BMPs and integrated N management can substantially reduce the climate footprint of vegetable production while maintaining economic viability. Future research should prioritize system-level assessments, long-term field studies including N<sub>2</sub>O emissions, and region-specific guidelines to support scalable, climate-smart vegetable production.</div></div>","PeriodicalId":34393,"journal":{"name":"Journal of Agriculture and Food Research","volume":"26 ","pages":"Article 102703"},"PeriodicalIF":6.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}