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Encapsulation of protein-based bioactive from black soldier fly for ruminant feeding 从黑兵蝇中封装蛋白质生物活性物质用于反刍动物饲养
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-08 DOI: 10.1016/j.jafr.2024.101325

The increasing global population and subsequent animal protein source demand necessitate the exploration of alternative feed sources for animal. Insects have emerged as a viable option due to their potential to provide high-quality proteins with minimal environmental impact. Black soldier fly (BSF), containing high nutrients such as protein and bioactive compound, influenced by growth stage and dietary factors, thus providing a substantial reservoir of essential amino acids and other bioactive compounds crucial for ruminant. However, these components are sensitive and require protection from the degradation in the rumen. Under the processing and delivery of those compounds, in addition to keeping and enhancing the functionality of the bioactive compounds, coating or protection should be implemented. Encapsulation, which has recently garnered attention for its potential technology in animal feed nutrition, offers promise in protecting sensitive components through their stability, protection, and controlled release within specific organs. This review article aims to investigate the application of encapsulation techniques for protein extracts derived from black soldier fly larvae to enhance their effectiveness and utilization in ruminant feeding. Additionally, the review consolidates studies on the transformation processes of the nutrition values of various edible insects, the method of processing black soldier fly, BSF protein extraction process, and the encapsulation technique. In conclusion, the encapsulation of protein extracts from black soldier fly larvae represents a promising BSF-derived protein to provide sustainable and effective alternatives for improving ruminant nutrition in the future.

全球人口的不断增长以及随之而来的动物蛋白质来源需求,使得人们有必要探索动物饲料的替代来源。昆虫因其在提供优质蛋白质的同时对环境影响最小的潜力而成为一种可行的选择。黑背天蝇(BSF)含有蛋白质和生物活性化合物等高营养成分,受生长阶段和饮食因素的影响,因此可提供大量对反刍动物至关重要的必需氨基酸和其他生物活性化合物。然而,这些成分都很敏感,需要得到保护,以免在瘤胃中降解。在加工和输送这些化合物的过程中,除了保持和增强生物活性化合物的功能外,还应该进行包衣或保护。最近,封装技术因其在动物饲料营养方面的潜在技术而备受关注,它有望通过稳定、保护和在特定器官内控制释放来保护敏感成分。这篇综述文章旨在研究从黑实蝇幼虫中提取的蛋白质提取物的封装技术应用,以提高其在反刍动物饲养中的有效性和利用率。此外,综述还整合了有关各种食用昆虫营养价值转化过程、黑兵蝇加工方法、黑兵蝇蛋白提取过程和封装技术的研究。总之,黑翅大实蝇幼虫蛋白提取物的封装技术是一种很有前景的黑翅大实蝇衍生蛋白,可在未来为改善反刍动物营养提供可持续的有效替代品。
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引用次数: 0
Phytochemicals screening, nutritional assessment and antioxidant activities of A. viridis L. and A. spinosus L. leaves: A comparative study A. viridis L. 和 A. spinosus L. 叶片的植物化学成分筛选、营养评估和抗氧化活性:比较研究
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-08 DOI: 10.1016/j.jafr.2024.101341

This study aims to compare the analysis of phytochemicals, nutrition, cytotoxicity, and antioxidant activity of A. viridis and A. spinosus leaves because they are commonly used as wild leafy vegetables worldwide and valuable for traditional medicinal purposes. To achieve these goals, the standard protocol was followed in each experiment. Comparative analysis revealed that the A. spinosus leaf had more phytochemicals, such as alkaloids, flavonoids, terpenoids, carbohydrates, glycosides, cardiac glycosides, amino acids, xanthoproteins, phenols, saponins, steroids, and coumarins, than A. viridis. Aqueous solvents were more effective in identifying the presence of phytochemicals than methanol and ethanol. A. viridis leaves contain higher amounts of carbohydrates (49 ± 2 mg gluc./g), protein (53 ± 3 mg BSA/g), calcium (2.67 ± 0.03 mg/kg), amino acids (7 essential and 5 non-essential), total phenol (21 ± 1 mg GAE/g), and high antioxidant activity (IC50 = 109.0 ± 0.2 μg/cm3) than A. spinosus. On the other hand, A. spinosus leaves contain higher ash (17.1 ± 0.2 %), total flavonoid (16 ± 1 mg QE/g), and total antioxidant capacity (50 ± 1 mg AA/g) than A. viridis. The findings of the heavy metals analysis revealed that the concentrations of zinc, manganese, iron, and chromium were below the recommended limits of FAO/WHO, whereas copper (in both species) and cadmium (in A. viridis) were over the acceptable levels. The leaves of both species showed significant cytotoxic activity (LC50 < 1000). The study concluded that A. viridis and A. spinosus are great sources of phytochemicals, nutrients, and natural antioxidants. The results also support the traditional medical uses of these plant species and suggest more investigation to find novel compounds for pharmaceutical applications.

本研究的目的是对比分析A. viridis和A. spinosus叶片的植物化学成分、营养、细胞毒性和抗氧化活性,因为这两种叶片是世界上常用的野生叶菜,具有传统的药用价值。为了实现这些目标,每个实验都遵循了标准方案。比较分析表明,A. spinosus 叶子比 A. viridis 含有更多的植物化学物质,如生物碱、黄酮类、萜类、碳水化合物、苷类、强心苷、氨基酸、黄原蛋白、酚类、皂苷、类固醇和香豆素。水溶剂比甲醇和乙醇更能有效地鉴定植物化学物质的存在。与 A. spinosus 相比,A. viridis 叶片含有更多的碳水化合物(49 ± 2 mg gluc./g)、蛋白质(53 ± 3 mg BSA/g)、钙(2.67 ± 0.03 mg/kg)、氨基酸(7 种必需氨基酸和 5 种非必需氨基酸)、总酚(21 ± 1 mg GAE/g)和较高的抗氧化活性(IC50 = 109.0 ± 0.2 μg/cm3)。另一方面,A. spinosus 叶子的灰分(17.1 ± 0.2 %)、总黄酮(16 ± 1 毫克 QE/克)和总抗氧化能力(50 ± 1 毫克 AA/克)均高于 A. viridis。重金属分析结果表明,锌、锰、铁和铬的浓度低于粮农组织/世卫组织的建议限值,而铜(在两种植物中)和镉(在金鸡菊中)则超过了可接受的水平。两个物种的叶片都显示出明显的细胞毒性活性(LC50 < 1000)。研究认为,A. viridis 和 A. spinosus 是植物化学物质、营养物质和天然抗氧化剂的重要来源。研究结果还支持了这些植物物种的传统医学用途,并建议开展更多研究,以找到可用于制药的新型化合物。
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引用次数: 0
An assessment of the vulnerability and adaptation potential of sugarcane production to water stress, southern Africa 评估南部非洲甘蔗生产对水资源压力的脆弱性和适应潜力
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-08 DOI: 10.1016/j.jafr.2024.101348

The high spatial variability of precipitation, heightened frequency of droughts and concomitant increases in exposure to water stress across southern Africa due to climate change, presents significant challenges for sugarcane production and the regional sugarcane production value chain. While production has intensified in the past few decades, yields have declined due to increased climatic variability and agronomic management approaches. Increased precipitation variability has enhanced sugarcane vulnerability to water stress and is likely to negatively affect yields. Combining crop simulations and relationships between sugarcane water use and observed rainfall, we introduce a crop productivity ratio (CPR) which assesses sugarcane water stress for six sugarcane mills across southern Africa. The CPR and simulation results were used to assess the adaptation potential or ‘space’ for mill areas that have varying rates of exposure and abilities to adapt to water stress. Simulation results were used to determine the long-term adaption potential of mill areas and to surmise the causes of yield declines. The results were used to offer recommendations to reduce vulnerabilities and enhance adaptation to water stress. We conclude that the amplification of inter-annual precipitation variability will enhance the exposure of sugarcane to water stress and require adaptation interventions. Adapting to external shocks is a multifaceted exercise that requires a holistic approach that includes every aspect of the sugarcane value chain.

由于气候变化,整个南部非洲降水量的空间变异性很大,干旱发生的频率增加,随之而来的是水压力的增加,这给甘蔗生产和区域甘蔗生产价值链带来了重大挑战。在过去几十年里,虽然甘蔗生产得到了加强,但由于气候变异性和农艺管理方法的增加,产量却有所下降。降水变异性的增加使甘蔗更容易受到水分胁迫的影响,并可能对产量产生负面影响。结合作物模拟和甘蔗用水量与观测降雨量之间的关系,我们引入了作物生产力比(CPR),用于评估南部非洲六个甘蔗种植园的甘蔗用水压力。作物生产力比和模拟结果被用于评估甘蔗种植区的适应潜力或 "空间",这些种植区对水分胁迫的暴露率和适应能力各不相同。模拟结果用于确定碾磨区的长期适应潜力,并推测产量下降的原因。根据模拟结果提出建议,以降低脆弱性并增强对水胁迫的适应能力。我们的结论是,年际降水变化的扩大将使甘蔗更容易受到水压力的影响,因此需要采取适应性干预措施。适应外部冲击是一项多方面的工作,需要采取包括甘蔗价值链各个方面的整体方法。
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引用次数: 0
Stability differences of quality traits in high-quality hybrid rice 优质杂交水稻品质性状的稳定性差异
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-07 DOI: 10.1016/j.jafr.2024.101346

Rice breeders have developed numerous hybrid rice varieties with high yield and improved quality over the years. However, limited information is available on the stability of quality traits in high-quality rice. This study determined the stability of quality traits in two high-quality hybrid rice varieties, Zhuliangyou 570 (ZLY570) and Huazheyou 261 (HZY261), across different ecological regions and agronomic practices through on-farm research and field experiments. The on-farm research was conducted across nine provinces in southern China in 2021–2023, and field experiments with two sowing dates and six nitrogen treatments were performed in Hunan Province in 2022 and 2023. Results indicated that in both on-farm research and field experiments, both varieties exhibited a high coefficient of variation (CV) in chalky grain rate and chalkiness degree, followed by head rice rate, amylose and protein contents, traits including brown and milled rice rates, and length-to-width ratio revealed a low CV. The chalky grain rate and chalkiness degree of both varieties displayed poor stability, followed by head rice rate and amylose content. In contrast, brown and milled rice rates, length-to-width ratio, and protein content demonstrated high stability in the genotype plus genotype × environment interaction biplot analysis. However, the maximum value of chalky grain rate and chalkiness degree were only 8.43 % and 1.83 % for ZLY570, and 9.12 % and 2.05 % for HZY261, respectively. Both varieties revealed a high range (R) in head rice rate and amylose content, but a low R in protein content. Furthermore, the head rice rate and amylose content varied widely, while protein content showed narrower variability. These results suggest that high-quality hybrid rice exhibits poor stability in terms of head rice rate and amylose content. In contrast, it demonstrates relatively high stability in brown and milled rice rates, length-to-width ratio, chalky grain rate, chalkiness degree, and protein content.

多年来,水稻育种人员培育出了许多高产、优质的杂交水稻品种。然而,有关优质水稻品质性状稳定性的信息却很有限。本研究通过田间研究和田间试验,测定了两个优质杂交水稻品种--珠连优 570(ZLY570)和华泽优 261(HZY261)在不同生态区域和农艺措施下的品质性状稳定性。2021-2023 年在中国南方九省开展了田间研究,2022 年和 2023 年在湖南省进行了两种播种期和六种氮肥处理的田间试验。结果表明,在田间研究和田间试验中,两个品种的垩白粒率和垩白度变异系数(CV)均较高,头米率、直链淀粉和蛋白质含量次之,糙米率、碾米率和长宽比等性状的变异系数较低。两个品种的垩白粒率和垩白度稳定性较差,头米率和直链淀粉含量次之。相比之下,糙米率和碾米率、长宽比和蛋白质含量在基因型加基因型×环境互作双图谱分析中表现出较高的稳定性。然而,ZLY570 的垩白粒率和垩白度的最大值分别只有 8.43 % 和 1.83 %,HZY261 为 9.12 % 和 2.05 %。这两个品种的出米率和直链淀粉含量的范围(R)都很高,但蛋白质含量的范围(R)却很低。此外,头米率和直链淀粉含量变化较大,而蛋白质含量变化较小。这些结果表明,优质杂交稻的头米率和直链淀粉含量稳定性较差。相比之下,优质杂交稻在糙米率和碾米率、长宽比、垩白粒率、垩白度和蛋白质含量方面的稳定性相对较高。
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引用次数: 0
Evaporative cooling with a wet fabric blanket for non-refrigerated horticultural produce transport: An experimental study 在非冷藏园艺产品运输中使用湿布毯进行蒸发冷却:实验研究
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-06 DOI: 10.1016/j.jafr.2024.101339

Due to the high cost of mechanical refrigeration, Evaporative Cooling (EC) can be an alternative technology for small farmers in developing countries, such as Thailand. This study aimed to experimentally investigate the performance of EC using a wet fabric blanket. A real-scale cargo chamber, commonly used in Thailand, was constructed, and equipped with axial fans to simulate airflow during transportation. Two pallets of test products (hollow plastic balls) were loaded into the cargo and covered with the wet blanket. During the experiment, the inlet air velocities varied from 0.8 m s−1 to 3.6 m s−1 while the constant climate conditions were maintained (29–30 °C and 70–73 %RH). The air and product temperatures and air relative humidity were measured every minute for 3 h using thermocouples and hygrometers, respectively. The proposed EC method allowed the air temperature to decrease by approximately 3–4 °C. When the inlet air velocity decreased, a lower temperature reduction was observed. Simplified heat and mass transfer models were developed to interpret the load temperature evolution from the inlet to the outlet positions at different air velocities. The comparison between the measured and calculated data revealed a maximum mean relative error of 1.2 %. The quality preservation performance was also evaluated based on lettuce mass loss. Lower mass loss was observed for the product stored inside the cargo chamber (<6 %) compared to those outside (8–10 %). This study suggests the potential use of a wet blanket as an EC cooling medium for a short-distance transport to enhance the cold chain performance in Thailand.

由于机械制冷成本高昂,蒸发冷却(EC)可以成为泰国等发展中国家小农户的替代技术。本研究旨在利用湿布毯对蒸发冷却的性能进行实验研究。研究人员建造了一个泰国常用的真实规模的货舱,并配备了轴流风扇,以模拟运输过程中的气流。两个托盘的测试产品(空心塑料球)被装入货舱,并覆盖湿布毯。实验期间,进气速度从 0.8 m s-1 到 3.6 m s-1 不等,同时保持恒定的气候条件(29-30 °C 和 70-73%RH)。使用热电偶和湿度计分别在 3 小时内每分钟测量一次空气和产品温度以及空气相对湿度。拟议的 EC 方法可使空气温度降低约 3-4 °C。当入口气流速度降低时,观察到的降温幅度也更小。我们建立了简化的传热和传质模型,以解释不同风速下从入口到出口位置的负载温度变化。测量数据和计算数据的比较显示,最大平均相对误差为 1.2%。还根据莴苣的质量损失评估了保质性能。观察发现,储存在货仓内的产品的质量损失(6%)低于储存在货仓外的产品(8-10%)。这项研究表明,湿毯作为 EC 冷却介质可用于短途运输,从而提高泰国的冷链性能。
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引用次数: 0
Food cold chain logistics and management: A review of current development and emerging trends 食品冷链物流和管理:当前发展和新趋势综述
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-05 DOI: 10.1016/j.jafr.2024.101343

Purpose

This study conducts a bibliometric analysis of Food Cold Chain Logistics and Management (FCCLM) literature to identify key discussions influencing its development and highlight current and emerging trends. The study aims to uncover major research themes, trends, and gaps, providing a comprehensive overview of FCCLM research.

Design/methodology/approach

Using bibliometric analysis via VOSviewer and Biblioshiny, data from over 114 academic publications in Scopus is examined to identify influential contributors and patterns in FCCLM literature. The study involves several stages, including data acquisition, preprocessing, and bibliometric assessments like co-occurrence, citation, and co-citation analyses.

Findings

The research reveals significant growth in FCCLM literature, particularly contributions from developed nations. The study identifies six primary research clusters: (1) Integrated Cold Chain and Logistics Management, (2) Sustainable Cold Chain Logistics, (3) Cold Chain Logistics for Food Safety, (4) Optimizing Food Storage and Shelf Life, (5) Blockchain and Digital Solutions in Food Supply Chain, and (6) Leveraging AI and RFID for Cost-Effective Quality Control. Key findings include the increasing emphasis on sustainability and technological advancements, such as RFID and IoT, that enhance traceability and operational efficiency. Despite these advancements, significant gaps remain in understanding the practical application of these technologies, the impact of climate change, and the need for better human factors and training in cold chain logistics.

Practical implications

The insights are crucial for researchers, policymakers, and practitioners involved in FCCLM. Understanding the current research landscape will help identify research needs, promote sustainable, tech-advanced practices, and foster collaboration to improve the global food supply chain's efficiency and resilience.

Originality

This study comprehensively reviews FCCLM literature, identifying trends and highlighting future research areas. It offers a unique perspective on the evolution of FCCLM research, emphasizing the importance of sustainability and technological advancements in overcoming challenges.

目的本研究对食品冷链物流与管理(FCCLM)文献进行了文献计量分析,以确定影响其发展的关键讨论,并突出当前和新出现的趋势。本研究旨在揭示主要的研究主题、趋势和差距,为 FCCLM 研究提供一个全面的概览。设计/方法/途径通过 VOSviewer 和 Biblioshiny 进行文献计量分析,对 Scopus 中超过 114 篇学术出版物的数据进行研究,以确定 FCCLM 文献中具有影响力的贡献者和模式。研究涉及多个阶段,包括数据采集、预处理和文献计量学评估,如共现分析、引文分析和共引分析等。研究确定了六个主要研究集群:(1) 集成冷链和物流管理,(2) 可持续冷链物流,(3) 食品安全冷链物流,(4) 优化食品储存和保质期,(5) 食品供应链中的区块链和数字解决方案,以及 (6) 利用人工智能和 RFID 实现经济高效的质量控制。主要发现包括:人们越来越重视可持续发展和技术进步,如 RFID 和物联网,这些技术可提高可追溯性和运营效率。尽管取得了这些进步,但在了解这些技术的实际应用、气候变化的影响以及改善冷链物流中的人为因素和培训需求方面仍存在巨大差距。了解当前的研究状况将有助于确定研究需求,推广可持续的先进技术实践,促进合作,从而提高全球食品供应链的效率和复原力。 独创性本研究全面回顾了有关冷链物流管理的文献,确定了趋势并强调了未来的研究领域。它以独特的视角审视了食品供应链管理研究的发展,强调了可持续性和技术进步在克服挑战方面的重要性。
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引用次数: 0
Understanding farmed salmon imports and e-commerce consumer satisfaction in China: A text mining approach 了解中国养殖三文鱼进口和电子商务消费者满意度:文本挖掘方法
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-05 DOI: 10.1016/j.jafr.2024.101342

Salmon, a luxury aquatic species, has seen a steady increase in imports in China. The rapid development of the Internet, the transformation of consumption behaviour, and the impact of the COVID-19 pandemic have prompted consumers to purchase salmon products from e-commerce platforms in China. In this context, understanding salmon import trends and consumer satisfaction with salmon e-commerce is critical to promoting and sustaining sales of salmon products through e-commerce platforms. Therefore, this study aims to investigate the import trend analysis of salmon in China as well as understand factors influencing consumer satisfaction with salmon e-commerce. We analysed 10,064 consumer comments out of 25,763 for this study after resampling the proportion of positive/negative comments. Sentiment analyser, Textblob, classified 65.2 % of consumer comments as positive, 21.7 % as negative, and 13.1 % as neutral. Additionally, Latent Dirichlet Allocation (LDA) topic modelling identified five areas critical for satisfaction with salmon e-commerce: purchase decision (19.5 % of the comments), eating experience (22.3 %), service quality and delivery (20.6 %), purchase history (17.5 %), and general product quality (20 %). Our findings indicate that service quality and delivery are the most critical factors affecting consumer satisfaction, whereas purchase history has the least impact. Consumers’ PLUS membership profile, salmon product style, and preservation form had a significant influence on consumer satisfaction, while the country of origin, weight and price of the product had no significant influence. Salmon e-commerce platforms need to improve logistic service and delivery quality to influence consumer satisfaction.

三文鱼作为一种奢侈的水产物种,在中国的进口量持续增长。互联网的快速发展、消费行为的转变以及 COVID-19 大流行的影响,促使消费者从中国的电子商务平台购买三文鱼产品。在此背景下,了解三文鱼进口趋势和消费者对三文鱼电子商务的满意度对于通过电子商务平台促进和维持三文鱼产品的销售至关重要。因此,本研究旨在调查中国三文鱼进口趋势分析,并了解影响消费者对三文鱼电子商务满意度的因素。我们对 25,763 条消费者评论中的 10,064 条进行了分析,并对正面/负面评论的比例进行了重新取样。情感分析仪 Textblob 将 65.2% 的消费者评论归类为正面评论,21.7% 归类为负面评论,13.1% 归类为中性评论。此外,Latent Dirichlet Allocation (LDA) 主题建模确定了对三文鱼电子商务满意度至关重要的五个方面:购买决策(占评论的 19.5%)、用餐体验(22.3%)、服务质量和配送(20.6%)、购买历史(17.5%)和一般产品质量(20%)。我们的研究结果表明,服务质量和配送是影响消费者满意度的最关键因素,而购买历史对其影响最小。消费者的 PLUS 会员资格、三文鱼产品风格和保存形式对消费者满意度有显著影响,而产品的原产国、重量和价格则没有显著影响。三文鱼电子商务平台需要改善物流服务和配送质量,以提高消费者满意度。
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引用次数: 0
Forecasting fish prices with an artificial neural network model during the tuna fraud 在金枪鱼欺诈案中利用人工神经网络模型预测鱼价
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-05 DOI: 10.1016/j.jafr.2024.101340

Agricultural price forecasting plays an important role in stabilising markets and ensuring food security. It provides insights for various stakeholders to optimise planting choices, allocate resources efficiently and mitigate potential risks. However, price forecasting during food safety incidents poses unique challenges. This study focused on a case of tuna fraud in Spain in 2017, which caused 105 people to fall ill and influenced consumer behaviour in fish purchases. To forecast fish prices during an incident of fraud, we used an artificial neural network model (ANN) based on the price of tuna and its substitutes, salmon and hake, as well as a communication index based on the number of posts regarding the tuna fraud from the social media platform X (formerly Twitter). ANN was compared with a threshold vector autoregressive model (TVAR), a classical time series econometric model that offers valuable insights into price dynamics. The results showed that, in the short term, TVAR offers a better price forecast for tuna and salmon, considering the impacts of the X platform. In the medium term, ANN outperformed TVAR. This study contributes to the ANN literature regarding agrifood price forecasting during food safety incidents.

农产品价格预测在稳定市场和确保粮食安全方面发挥着重要作用。它为各利益相关方优化种植选择、有效分配资源和降低潜在风险提供了真知灼见。然而,在食品安全事件中进行价格预测却面临着独特的挑战。本研究侧重于 2017 年西班牙的一起金枪鱼欺诈案,该案导致 105 人患病,并影响了消费者的水产品购买行为。为了预测欺诈事件期间的水产品价格,我们使用了一个人工神经网络模型(ANN),该模型基于金枪鱼及其替代品三文鱼和无须鳕的价格,以及基于社交媒体平台 X(原 Twitter)上有关金枪鱼欺诈事件的帖子数量的传播指数。ANN 与阈值向量自回归模型(TVAR)进行了比较,后者是一种经典的时间序列计量经济模型,可为价格动态提供有价值的见解。结果表明,考虑到 X 平台的影响,TVAR 在短期内能更好地预测金枪鱼和鲑鱼的价格。从中期来看,ANN 的表现优于 TVAR。本研究为有关食品安全事件期间农产品价格预测的 ANN 文献做出了贡献。
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引用次数: 0
Magnesium deficiency induced leaf chlorosis affects plant growth, mineral concentration and fruit quality in field pomelo trees 缺镁引起的叶片萎黄对大田柚子树的植株生长、矿物质浓度和果实品质的影响
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-08-03 DOI: 10.1016/j.jafr.2024.101338

Healthy trees are considered the foundation for high-quality pomelo production; however, leaf chlorosis frequently happens in pomelo orchards, and the reasons and adverse effects on plant growth and fruit quality are poorly understood. We conducted a field investigation with six paired groups between the yellow and green leaves of pomelo trees to analyze the growth, mineral and quality characteristics in different leaf positions and fruit parts. Compared with green pomelo trees, the concentrations of nitrogen (N), phosphorus (P), calcium (Ca), and magnesium (Mg) were decreased and increased potassium (K) in various leaf organs of yellow pomelo trees, resulting in accumulated non-structural carbohydrates and reduced growth variables (including SPAD value as relative chlorophyll content, dry weight, leaf area, water content, and specific leaf weight), which was amplified with fruit than without fruit. Also, there was the highest positive correlation between leaf Mg concentration and SPAD value, further confirmed by the path analysis and random forest analysis, implying that Mg is the main factor affecting leaf chlorosis. Moreover, leaf chlorosis also affected fruit quality, in which the concentrations of N, P and Ca were decreased and increased K and Mg, with lower sugar content (including total soluble sugar, sucrose, and total soluble solids) and higher acidity content (including pH, vitamin C, and titratable acidity), resulting in decreased total soluble solids/titratable acidity and antioxidant capacity (less phenol content). The positive correlations between leaf Mg concentration and fruit quality parameters were also observed. However, these measured parameters were highly separated and accounted for 53.1 % of leaf and fruit organs between the yellow and green leaves of pomelo trees. Overall, our results demonstrate that Mg deficiency induced leaf chlorosis affects plant growth and fruit quality in pomelo trees, that SPAD can be used to monitor leaf Mg status, and these findings provide the basis for optimized nutrient management in sustainable pomelo production.

健康的柚树是优质柚子生产的基础;然而,柚园中经常出现叶片萎黄病,其原因及对植物生长和果实品质的不利影响却鲜为人知。我们对柚树的黄叶和绿叶进行了六组配对田间调查,分析了不同叶片位置和果实部位的生长、矿物质和品质特征。与绿叶柚树相比,黄叶柚树各叶片器官中氮(N)、磷(P)、钙(Ca)和镁(Mg)的浓度降低,钾(K)的浓度升高,导致非结构性碳水化合物积累和生长变量(包括作为相对叶绿素含量的SPAD值、干重、叶面积、含水量和比叶重)降低,有果比无果的生长变量放大。此外,叶片镁浓度与 SPAD 值之间的正相关性最高,路径分析和随机森林分析进一步证实了这一点,即镁是影响叶片萎黄病的主要因素。此外,叶片萎黄病还影响果实品质,其中氮、磷和钙的浓度降低,钾和镁的浓度升高,含糖量(包括总可溶性糖、蔗糖和总可溶性固形物)降低,酸度(包括 pH 值、维生素 C 和可滴定酸度)升高,导致总可溶性固形物/可滴定酸度和抗氧化能力(酚含量降低)降低。叶片镁浓度与果实质量参数之间也呈正相关。不过,这些测量参数高度分离,占柚子树黄叶和绿叶之间叶片和果实器官的 53.1%。总之,我们的研究结果表明,镁缺乏引起的叶片萎黄会影响柚子树的植株生长和果实品质,SPAD 可用于监测叶片的镁状态,这些发现为柚子可持续生产中的优化养分管理提供了依据。
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引用次数: 0
Subcritical water extraction of mangrove fruit extract (Sonneratia alba) and its antioxidant activity, network pharmacology, and molecular connectivity studies 亚临界水提取红树林果实提取物(Sonneratia alba)及其抗氧化活性、网络药理学和分子连接性研究
IF 4.8 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-07-30 DOI: 10.1016/j.jafr.2024.101334

Functional foods containing antioxidant molecules can limit the prevalence of various diseases, increase immunity, slow aging, and improve physical appearance. The aim of this study was to use non-toxic to prepare extracts from the Sonneratia alba fruit, determine the phytochemical composition, and assess the biological activity via in silico and in vitro antioxidant capability. Variations in temperatures and times showed that the conditions for best phytochemicals and antioxidant activity were 100 °C and 10 min. The highest values were 23.33 mg quercetin equivalent/g (flavonoids), 1.24 mg tannic acid equivalent/g (tannins), and IC50 = 62.38 ppm (antioxidant activity). Gas chromatography displayed 7 peaks that were attributed to 21 possible compounds. Molecular docking analysis results showed that the mechanism of three of the compounds (1) Ethyl iso-allocholate, (2) Gibb-3-ene-1,10-dicarboxylic acid,2,4a,7-trihydroxy-1-methyl-8-methylene-,1,4a-lactone,10-methyl ester,(1a,2β,4aa,4bβ,10β)-, and (3) Pregn-4-ene-3,20-dione,17,21-dihydroxy-,bis(O-methyloxime) could be due to their binding via hydrogen bonding to 3 reactive oxygen species (ROS) proteins (nitric oxide synthases). Subcritical water extract of Mangrove fruit (S. alba) has potential as a functional food that has antioxidant activity in silico and in vitro.

含有抗氧化分子的功能性食品可以限制各种疾病的发病率,提高免疫力,延缓衰老,改善体形。本研究旨在使用无毒的方法制备白桑果提取物,确定其植物化学成分,并通过硅学和体外抗氧化能力评估其生物活性。温度和时间的变化表明,植物化学成分和抗氧化活性最佳的条件是 100 °C 和 10 分钟。最高值为 23.33 毫克槲皮素当量/克(黄酮类化合物)、1.24 毫克单宁酸当量/克(单宁酸)和 IC50 = 62.38 ppm(抗氧化活性)。气相色谱法显示出 7 个峰值,可归结为 21 种可能的化合物。分子对接分析结果表明,其中三种化合物(1)异异胆酸乙酯;(2)吉卜-3-烯-1,10-二羧酸,2,4a,7-三羟基-1-甲基-8-亚甲基-,1,4a-内酯,10-甲基酯、(1a,2β,4aa,4bβ,10β)-,以及 (3) 孕甾-4-烯-3,20-二酮,17,21-二羟基,双(O-甲基肟)可能是由于它们通过氢键与 3 种活性氧(ROS)蛋白(一氧化氮合酶)结合所致。红树果实(S. alba)的亚临界水提取物具有作为一种功能性食品的潜力,它在硅学和体外试验中都具有抗氧化活性。
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引用次数: 0
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Journal of Agriculture and Food Research
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