Environmental Science and Ecotechnology最新文献_第7页

Redox regulation for sustainable water purification and risk management 可持续水净化和风险管理的氧化还原调节

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-05-01 DOI: 10.1016/j.ese.2025.100563

Ai-Jie Wang , Rui-Feng Yan , Ke Shi , Hao-Yi Cheng , Jing-Long Han , Bin Liang

Sustainable detoxification and advanced treatment of toxic organic pollutants (TOPs) in wastewater are essential for water reclamation and ecosystem security. Although biological treatment is a low-carbon and eco-friendly approach for TOPs degradation, its effectiveness is often limited by the high toxicity and recalcitrance of TOPs. Oxidative and reductive reactions can degrade TOPs according to their intrinsic redox potentials. However, conventional biological or chemical oxidation treatment often fails to efficiently or purposefully cleave key functional groups, which leads to unsatisfactory performance of biological reactions or excessive chemical oxidation costs. This perspective proposes redox regulation as a strategy to moderately catalyse the oxidation or reduction of TOPs and thereby generate low toxicity and increased biodegradable intermediates, which will improve subsequent biological treatment. We summarize strong redox regulation techniques, including advanced oxidation and reduction processes, and weak redox regulation through low-energy electrical potential, along with the corresponding mechanisms and applications. Additionally, we explore the integration of redox regulation with biological treatment, either in a sequential mode or in situ. This study emphasizes the need for future research to focus on targeted and durable catalytic detoxification processes and to optimize balancing the carbon footprint, process control, operational efficiency, and economic feasibility. By integrating chemical reactions with microbial metabolism, redox regulation has the potential to transform wastewater treatment from isolated process optimization to a holistic approach. This perspective advocates for innovation of conventional wastewater detoxification technologies to achieve sustainable water purification and ecological risk control.

废水中有毒有机污染物（TOPs）的可持续解毒和深度处理对水回收和生态系统安全至关重要。虽然生物处理是一种低碳、环保的降解TOPs的方法，但其效果往往受到TOPs的高毒性和难降解性的限制。氧化和还原反应可以根据其固有的氧化还原电位来降解TOPs。然而，传统的生物或化学氧化处理往往不能有效或有目的地切割关键官能团，从而导致生物反应性能不理想或化学氧化成本过高。这一观点提出，氧化还原调节是一种适度催化TOPs氧化或还原的策略，从而产生低毒性和增加可生物降解的中间体，这将改善后续的生物处理。本文综述了强氧化还原调控技术，包括高级氧化还原过程和低能量电位弱氧化还原调控技术，以及相应的机制和应用。此外，我们探索了氧化还原调控与生物处理的整合，无论是在顺序模式还是在原位。本研究强调了未来的研究需要关注有针对性和持久的催化解毒过程，并优化平衡碳足迹，过程控制，操作效率和经济可行性。通过将化学反应与微生物代谢相结合，氧化还原调控有可能将废水处理从孤立的过程优化转变为整体方法。这一观点提倡创新传统的废水解毒技术，以实现可持续的水净化和生态风险控制。

{"title":"Redox regulation for sustainable water purification and risk management","authors":"Ai-Jie Wang , Rui-Feng Yan , Ke Shi , Hao-Yi Cheng , Jing-Long Han , Bin Liang","doi":"10.1016/j.ese.2025.100563","DOIUrl":"10.1016/j.ese.2025.100563","url":null,"abstract":"<div><div>Sustainable detoxification and advanced treatment of toxic organic pollutants (TOPs) in wastewater are essential for water reclamation and ecosystem security. Although biological treatment is a low-carbon and eco-friendly approach for TOPs degradation, its effectiveness is often limited by the high toxicity and recalcitrance of TOPs. Oxidative and reductive reactions can degrade TOPs according to their intrinsic redox potentials. However, conventional biological or chemical oxidation treatment often fails to efficiently or purposefully cleave key functional groups, which leads to unsatisfactory performance of biological reactions or excessive chemical oxidation costs. This perspective proposes redox regulation as a strategy to moderately catalyse the oxidation or reduction of TOPs and thereby generate low toxicity and increased biodegradable intermediates, which will improve subsequent biological treatment. We summarize strong redox regulation techniques, including advanced oxidation and reduction processes, and weak redox regulation through low-energy electrical potential, along with the corresponding mechanisms and applications. Additionally, we explore the integration of redox regulation with biological treatment, either in a sequential mode or <em>in situ</em>. This study emphasizes the need for future research to focus on targeted and durable catalytic detoxification processes and to optimize balancing the carbon footprint, process control, operational efficiency, and economic feasibility. By integrating chemical reactions with microbial metabolism, redox regulation has the potential to transform wastewater treatment from isolated process optimization to a holistic approach. This perspective advocates for innovation of conventional wastewater detoxification technologies to achieve sustainable water purification and ecological risk control.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100563"},"PeriodicalIF":14.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A life cycle risk assessment of nanopesticides in freshwater 淡水中纳米农药的生命周期风险评估

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-05-01 DOI: 10.1016/j.ese.2025.100565

Mingyan Ke , Keshuo Zhang , Andrea L. Hicks , Fan Wu , Jing You

Conventional ecological risk assessments prioritize downstream anthropogenic impacts, overlooking risks arising from upstream processes involving highly hazardous substances and indirect emissions. This narrow focus obscures high-risk hotspots and renders traditional methodologies ill-suited for evaluating novel chemical entities. Nanopesticides, designed for targeted delivery of pesticidal active ingredients, are increasingly deployed to enhance efficiency, yet their altered environmental fate and transport dynamics may reshape end-of-life risks while their full lifecycle impacts remain uncharacterized. Here, we address this gap using imidacloprid (IMI) and its nano-encapsulated variant (nano-IMI) as case studies. By applying life cycle assessment and integrating the USEtox ecotoxicity model with the nano-specific SimpleBox4Nano framework, we quantify "cradle-to-gate" environmental impacts and derive substance-specific ecotoxicity metrics, enabling systematic characterization of end-of-life risks associated with these formulations. Production-stage ecological risks of nano-IMI (4.63 × 10³ CTUe) are approximately four times greater than those for conventional IMI (1.18 × 10³ CTUe). However, end-of-life freshwater ecological risks from nano-IMI emissions (0.012–6.93 × 10⁴ CTUe) are 2–5 orders of magnitude lower compared with IMI (1.59 × 10³–6.13 × 10⁶ CTUe), accounting for rainfall variability, toxicity data selection, fate, and environmental transport scenarios. Under equivalent rainfall conditions, nano-IMI exhibited up to three orders of magnitude lower integrated life-cycle freshwater ecological risks, underscoring its potential as an environmentally preferable alternative to conventional IMI. This research introduces a comprehensive and novel methodology for evaluating engineered nanomaterial alternatives across realistic environmental scenarios, providing essential insights into nanopesticide risk assessment throughout their lifecycle.

传统的生态风险评估优先考虑下游的人为影响，忽略了涉及高度有害物质和间接排放的上游过程产生的风险。这种狭隘的焦点模糊了高风险的热点，使得传统的方法不适合评估新的化学实体。纳米农药是为靶向递送农药活性成分而设计的，越来越多地用于提高效率，然而，它们改变的环境命运和运输动态可能会重塑生命末期的风险，而其整个生命周期的影响仍不清楚。在这里，我们使用吡虫啉（IMI）及其纳米封装变体（nano-IMI）作为案例研究来解决这一差距。通过应用生命周期评估，并将USEtox生态毒性模型与纳米特异性SimpleBox4Nano框架相结合，我们量化了“从摇篮到闸门”的环境影响，并得出了特定物质的生态毒性指标，从而能够系统地表征与这些配方相关的生命末期风险。纳米IMI （4.63 × 103 CTUe）的生产阶段生态风险约为常规IMI （1.18 × 103 CTUe）的4倍。然而，考虑到降雨变率、毒性数据选择、命运和环境运输情景，纳米IMI排放（0.012-6.93 × 104 CTUe）的生命末期淡水生态风险比IMI （1.59 × 103-6.13 × 106 CTUe）低2-5个数量级。在相同的降雨条件下，纳米IMI的综合生命周期淡水生态风险降低了3个数量级，强调了其作为传统IMI的环保替代品的潜力。本研究介绍了一种全面而新颖的方法，用于评估工程纳米材料在现实环境中的替代品，为纳米农药在其整个生命周期中的风险评估提供了重要的见解。

{"title":"A life cycle risk assessment of nanopesticides in freshwater","authors":"Mingyan Ke , Keshuo Zhang , Andrea L. Hicks , Fan Wu , Jing You","doi":"10.1016/j.ese.2025.100565","DOIUrl":"10.1016/j.ese.2025.100565","url":null,"abstract":"<div><div>Conventional ecological risk assessments prioritize downstream anthropogenic impacts, overlooking risks arising from upstream processes involving highly hazardous substances and indirect emissions. This narrow focus obscures high-risk hotspots and renders traditional methodologies ill-suited for evaluating novel chemical entities. Nanopesticides, designed for targeted delivery of pesticidal active ingredients, are increasingly deployed to enhance efficiency, yet their altered environmental fate and transport dynamics may reshape end-of-life risks while their full lifecycle impacts remain uncharacterized. Here, we address this gap using imidacloprid (IMI) and its nano-encapsulated variant (nano-IMI) as case studies. By applying life cycle assessment and integrating the USEtox ecotoxicity model with the nano-specific SimpleBox4Nano framework, we quantify \"cradle-to-gate\" environmental impacts and derive substance-specific ecotoxicity metrics, enabling systematic characterization of end-of-life risks associated with these formulations. Production-stage ecological risks of nano-IMI (4.63 × 10<sup>3</sup> CTUe) are approximately four times greater than those for conventional IMI (1.18 × 10<sup>3</sup> CTUe). However, end-of-life freshwater ecological risks from nano-IMI emissions (0.012–6.93 × 10<sup>4</sup> CTUe) are 2–5 orders of magnitude lower compared with IMI (1.59 × 10<sup>3</sup>–6.13 × 10<sup>6</sup> CTUe), accounting for rainfall variability, toxicity data selection, fate, and environmental transport scenarios. Under equivalent rainfall conditions, nano-IMI exhibited up to three orders of magnitude lower integrated life-cycle freshwater ecological risks, underscoring its potential as an environmentally preferable alternative to conventional IMI. This research introduces a comprehensive and novel methodology for evaluating engineered nanomaterial alternatives across realistic environmental scenarios, providing essential insights into nanopesticide risk assessment throughout their lifecycle.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100565"},"PeriodicalIF":14.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging technologies for detecting antibiotics in aquaculture wastewater: A critical review 水产养殖废水中抗生素检测新技术综述

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-05-01 DOI: 10.1016/j.ese.2025.100572

Xinyu Chang , Junchi Cui , Guihua Wang , Shujuan Meng , Lingling Chen , Meng Zhang

Antibiotic release and transfer within environmental systems significantly impact ecological stability and human health, posing considerable safety risks. Consequently, accurate and efficient detection methods for antibiotics, particularly within complex environmental matrices, are essential. A growing body of research is focusing on antibiotic pollution in wastewater and other environmental settings. Nevertheless, pervasive matrix interferences in complex water matrices and the inherent limitations of standalone analytical approaches in sensitivity, detection range, and other performance metrics underscore the demand for more robust and versatile detection platforms. Here we show a comprehensive overview and critical assessment of antibiotic contaminants, examining their sources, environmental distribution, and current detection methods. We focus on emerging antibiotic detection technologies, comparing their performance and highlighting suitable application contexts. Furthermore, we discuss fundamental principles and historical advancements in antibiotic detection and analytical methodologies. Finally, we identify significant challenges for antibiotic detection in complex environments, suggest viable strategies for future improvements, and outline promising research directions. This review not only provides essential guidance for advancing environmental antibiotic monitoring but also sheds light on the development of a strategic framework for robust, integrated platforms enabling multiplexed antibiotic monitoring in challenging environmental water matrices.

抗生素在环境系统内的释放和转移严重影响生态稳定和人类健康，构成相当大的安全风险。因此，准确和有效的抗生素检测方法，特别是在复杂的环境基质中，是必不可少的。越来越多的研究集中在废水和其他环境环境中的抗生素污染。然而，在复杂的水矩阵中普遍存在的矩阵干扰，以及独立分析方法在灵敏度、检测范围和其他性能指标方面的固有局限性，强调了对更强大、更通用的检测平台的需求。在这里，我们展示了抗生素污染物的全面概述和关键评估，检查它们的来源，环境分布和当前的检测方法。我们关注新兴的抗生素检测技术，比较它们的性能并强调合适的应用环境。此外，我们讨论了抗生素检测和分析方法的基本原理和历史进展。最后，我们确定了在复杂环境中抗生素检测的重大挑战，提出了未来改进的可行策略，并概述了有前途的研究方向。这篇综述不仅为推进环境抗生素监测提供了必要的指导，而且还为在具有挑战性的环境水基质中实现多路抗生素监测的强大综合平台的战略框架的发展提供了线索。

{"title":"Emerging technologies for detecting antibiotics in aquaculture wastewater: A critical review","authors":"Xinyu Chang , Junchi Cui , Guihua Wang , Shujuan Meng , Lingling Chen , Meng Zhang","doi":"10.1016/j.ese.2025.100572","DOIUrl":"10.1016/j.ese.2025.100572","url":null,"abstract":"<div><div>Antibiotic release and transfer within environmental systems significantly impact ecological stability and human health, posing considerable safety risks. Consequently, accurate and efficient detection methods for antibiotics, particularly within complex environmental matrices, are essential. A growing body of research is focusing on antibiotic pollution in wastewater and other environmental settings. Nevertheless, pervasive matrix interferences in complex water matrices and the inherent limitations of standalone analytical approaches in sensitivity, detection range, and other performance metrics underscore the demand for more robust and versatile detection platforms. Here we show a comprehensive overview and critical assessment of antibiotic contaminants, examining their sources, environmental distribution, and current detection methods. We focus on emerging antibiotic detection technologies, comparing their performance and highlighting suitable application contexts. Furthermore, we discuss fundamental principles and historical advancements in antibiotic detection and analytical methodologies. Finally, we identify significant challenges for antibiotic detection in complex environments, suggest viable strategies for future improvements, and outline promising research directions. This review not only provides essential guidance for advancing environmental antibiotic monitoring but also sheds light on the development of a strategic framework for robust, integrated platforms enabling multiplexed antibiotic monitoring in challenging environmental water matrices.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100572"},"PeriodicalIF":14.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward accurate and scalable rainfall estimation using surveillance camera data and a hybrid deep-learning framework 使用监控摄像头数据和混合深度学习框架进行准确和可扩展的降雨估计

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-05-01 DOI: 10.1016/j.ese.2025.100562

Fiallos-Salguero Manuel , Soon-Thiam Khu , Jingyu Guan , Mingna Wang

Rainfall measurement at high quality and spatiotemporal resolution is essential for urban hydrological modeling and effective stormwater management. However, traditional rainfall measurement methods face limitations regarding spatial coverage, temporal resolution, and data accessibility, particularly in urban settings. Here, we show a novel rainfall estimation framework that leverages surveillance cameras to enhance estimation accuracy and spatiotemporal data coverage. Our hybrid approach consists of two complementary modules: the first employs an image-quality signature technique to detect rain streaks from video frames and selects optimal regions of interest (ROIs). The second module integrates depthwise separable convolution (DSC) layers with gated recurrent units (GRU) in a regression model to accurately estimate rainfall intensity using these ROIs. We evaluate the framework using video data from two locations with distinct rainfall patterns and environmental conditions. The DSC–GRU model achieves high predictive performance, with coefficient of determination (R²) values ranging from 0.89 to 0.93 when validated against rain gauge measurements. Remarkably, the model maintains strong performance during daytime and nighttime conditions, outperforming existing video-based rainfall estimation methods and demonstrating robust adaptability across variable environmental scenarios. The model's lightweight architecture facilitates efficient training and deployment, enabling practical real-time urban rainfall monitoring. This work represents a substantial advancement in rainfall estimation technology, significantly reducing estimation errors and expanding measurement coverage, and provides a practical, low-cost solution for urban hydrological monitoring.

高质量和高时空分辨率的降雨测量对于城市水文建模和有效的雨水管理至关重要。然而，传统的降雨测量方法在空间覆盖、时间分辨率和数据可及性方面存在局限性，特别是在城市环境中。在这里，我们展示了一种新的降雨估计框架，该框架利用监控摄像机来提高估计精度和时空数据覆盖率。我们的混合方法由两个互补的模块组成：第一个模块采用图像质量签名技术从视频帧中检测雨纹并选择最佳感兴趣区域（roi）。第二个模块将深度可分离卷积（DSC）层与门控循环单元（GRU）集成在回归模型中，以使用这些roi准确估计降雨强度。我们使用来自两个具有不同降雨模式和环境条件的地点的视频数据来评估该框架。DSC-GRU模型具有很高的预测性能，与雨量计测量值进行验证时，决定系数（R2）值在0.89至0.93之间。值得注意的是，该模型在白天和夜间条件下都保持了较强的性能，优于现有的基于视频的降雨估计方法，并展示了对各种环境情景的强大适应性。该模型的轻量级架构有助于有效的培训和部署，实现实际的实时城市降雨监测。这项工作代表了降雨估算技术的实质性进步，显著降低了估算误差，扩大了测量范围，为城市水文监测提供了一种实用、低成本的解决方案。

{"title":"Toward accurate and scalable rainfall estimation using surveillance camera data and a hybrid deep-learning framework","authors":"Fiallos-Salguero Manuel , Soon-Thiam Khu , Jingyu Guan , Mingna Wang","doi":"10.1016/j.ese.2025.100562","DOIUrl":"10.1016/j.ese.2025.100562","url":null,"abstract":"<div><div>Rainfall measurement at high quality and spatiotemporal resolution is essential for urban hydrological modeling and effective stormwater management. However, traditional rainfall measurement methods face limitations regarding spatial coverage, temporal resolution, and data accessibility, particularly in urban settings. Here, we show a novel rainfall estimation framework that leverages surveillance cameras to enhance estimation accuracy and spatiotemporal data coverage. Our hybrid approach consists of two complementary modules: the first employs an image-quality signature technique to detect rain streaks from video frames and selects optimal regions of interest (ROIs). The second module integrates depthwise separable convolution (DSC) layers with gated recurrent units (GRU) in a regression model to accurately estimate rainfall intensity using these ROIs. We evaluate the framework using video data from two locations with distinct rainfall patterns and environmental conditions. The DSC–GRU model achieves high predictive performance, with coefficient of determination (R<sup>2</sup>) values ranging from 0.89 to 0.93 when validated against rain gauge measurements. Remarkably, the model maintains strong performance during daytime and nighttime conditions, outperforming existing video-based rainfall estimation methods and demonstrating robust adaptability across variable environmental scenarios. The model's lightweight architecture facilitates efficient training and deployment, enabling practical real-time urban rainfall monitoring. This work represents a substantial advancement in rainfall estimation technology, significantly reducing estimation errors and expanding measurement coverage, and provides a practical, low-cost solution for urban hydrological monitoring.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100562"},"PeriodicalIF":14.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic toxicity mechanisms of 6PPD and 6PPD-Quinone in zebrafish 6PPD和6PPD醌对斑马鱼的慢性毒性机制

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-05-01 DOI: 10.1016/j.ese.2025.100567

Fang Jiao , Yang Zhao , Qiang Yue , Qi Wang , Zhongzhi Li , Wanjing Lin , Lingxi Han , Liangfu Wei

N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) and its oxidation derivative, 6PPD-quinone (6PPDQ), have been extensively detected in environmental and biological samples, raising significant concerns regarding their chronic aquatic toxicity at environmentally relevant concentrations. However, the underlying mechanisms driving this chronic toxicity remain largely unexplored. Here we show that zebrafish exposed to 6PPD and 6PPDQ exhibit distinct toxicokinetic profiles, with 6PPD preferentially accumulating in the liver and 6PPDQ predominantly targeting the brain. Exposure to both compounds impaired zebrafish growth, induced hepatic damage, and disrupted locomotor behavior. Transcriptomic analysis of liver tissue revealed disturbances in lipid and carbohydrate metabolic pathways in both treatment groups, with distinct differences in gene expression patterns and biochemical responses between 6PPD and 6PPDQ. Specifically, both compounds downregulated peroxisome proliferator-activated receptor gamma (PPARγ) and elevated the expression of pro-inflammatory cytokines (TNF-α and IL-6). Molecular dynamics simulations and surface plasmon resonance experiments further demonstrated that hepatotoxicity was associated with direct binding of these compounds to PPARγ, a critical regulator of lipid metabolism and inflammation. Our findings highlight the hepatotoxic risks of 6PPD and 6PPDQ to aquatic life. Importantly, 6PPDQ exhibited greater toxicity compared to 6PPD, emphasizing an urgent need for targeted environmental controls and regulatory actions to mitigate ecological harm and potential public health consequences.

N-（1,3-二甲基丁基）-N ' -苯基-对苯二胺（6PPD）及其氧化衍生物6PPD-醌（6PPDQ）已在环境和生物样品中广泛检测到，引起了对其在环境相关浓度下的慢性水生毒性的重大关注。然而，驱动这种慢性毒性的潜在机制在很大程度上仍未被探索。本研究表明，暴露于6PPD和6PPDQ的斑马鱼表现出不同的毒性动力学特征，6PPD优先在肝脏积累，而6PPDQ主要针对大脑。暴露于这两种化合物会损害斑马鱼的生长，引起肝损伤，并扰乱运动行为。肝脏组织转录组学分析显示，两个治疗组的脂质和碳水化合物代谢途径均出现紊乱，6PPD和6PPDQ在基因表达模式和生化反应方面存在明显差异。具体来说，这两种化合物都下调过氧化物酶体增殖物激活受体γ (PPARγ)并升高促炎细胞因子（TNF-α和IL-6）的表达。分子动力学模拟和表面等离子体共振实验进一步证明，肝毒性与这些化合物与PPARγ的直接结合有关，PPARγ是脂质代谢和炎症的关键调节因子。我们的研究结果强调了6PPD和6PPDQ对水生生物的肝毒性风险。重要的是，与6PPD相比，6PPDQ表现出更大的毒性，强调迫切需要有针对性的环境控制和监管行动，以减轻生态危害和潜在的公共卫生后果。

{"title":"Chronic toxicity mechanisms of 6PPD and 6PPD-Quinone in zebrafish","authors":"Fang Jiao , Yang Zhao , Qiang Yue , Qi Wang , Zhongzhi Li , Wanjing Lin , Lingxi Han , Liangfu Wei","doi":"10.1016/j.ese.2025.100567","DOIUrl":"10.1016/j.ese.2025.100567","url":null,"abstract":"<div><div>N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) and its oxidation derivative, 6PPD-quinone (6PPDQ), have been extensively detected in environmental and biological samples, raising significant concerns regarding their chronic aquatic toxicity at environmentally relevant concentrations. However, the underlying mechanisms driving this chronic toxicity remain largely unexplored. Here we show that zebrafish exposed to 6PPD and 6PPDQ exhibit distinct toxicokinetic profiles, with 6PPD preferentially accumulating in the liver and 6PPDQ predominantly targeting the brain. Exposure to both compounds impaired zebrafish growth, induced hepatic damage, and disrupted locomotor behavior. Transcriptomic analysis of liver tissue revealed disturbances in lipid and carbohydrate metabolic pathways in both treatment groups, with distinct differences in gene expression patterns and biochemical responses between 6PPD and 6PPDQ. Specifically, both compounds downregulated peroxisome proliferator-activated receptor gamma (PPARγ) and elevated the expression of pro-inflammatory cytokines (TNF-α and IL-6). Molecular dynamics simulations and surface plasmon resonance experiments further demonstrated that hepatotoxicity was associated with direct binding of these compounds to PPARγ, a critical regulator of lipid metabolism and inflammation. Our findings highlight the hepatotoxic risks of 6PPD and 6PPDQ to aquatic life. Importantly, 6PPDQ exhibited greater toxicity compared to 6PPD, emphasizing an urgent need for targeted environmental controls and regulatory actions to mitigate ecological harm and potential public health consequences.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100567"},"PeriodicalIF":14.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pH-responsive production of hydroxyl radical in Fenton process Fenton过程中羟基自由基对ph值的响应

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-05-01 DOI: 10.1016/j.ese.2025.100566

Pengyi Wang , Fan Kang , Xiangbin Huang , Zhipeng Luo , Jing Zou , Min Yang , Meng Sun , Xin Yu , Huabin Zeng

Efficient management of temporal latency and spatial heterogeneity remains a critical challenge in sensor-based pH regulation for smart water management, primarily due to inherent response delays and mass transfer constraints. In oxidation systems with dynamic pH environments, delayed responses can lead to issues such as cyanide release, unwanted side reactions, or pipe damage. To address these challenges, we propose a “pause-then-adjust” control strategy, exploiting the pH-responsive generation of hydroxyl radicals (^•OH) in a modified Fenton reaction system. This system utilizes hydroxylamine as an electron donor and ethylenediaminetetraacetic acid (EDTA) as a stabilizer for iron ions. Within the pH range of 7.0–10.0, the coexistence of [Fe²⁺-EDTA]²⁻ and [Fe³⁺-OH-EDTA]²⁻ complexes facilitates efficient electron transfer, resulting in the selective and sustained production of ^•OH radicals. The inherent pH-responsiveness of this strategy enables rapid and spatially coherent adjustments, offering a robust supplementary method for addressing complex and evolving requirements in advanced water treatment systems.

有效管理时间延迟和空间异质性仍然是基于传感器的智能水管理pH调节的关键挑战，主要是由于固有的响应延迟和传质限制。在具有动态pH环境的氧化系统中，延迟反应可能导致氰化物释放、不良副反应或管道损坏等问题。为了解决这些挑战，我们提出了一种“暂停然后调整”的控制策略，利用改进的芬顿反应体系中羟基自由基（•OH）的ph响应生成。该系统利用羟胺作为电子供体，乙二胺四乙酸（EDTA）作为铁离子的稳定剂。在7.0 ~ 10.0的pH范围内，[Fe2+-EDTA]2−和[Fe3+-OH-EDTA]2−配合物的共存有利于有效的电子转移，从而选择性地持续产生•OH自由基。该策略固有的ph响应性使快速和空间一致的调整成为可能，为解决高级水处理系统中复杂和不断变化的要求提供了一种强大的补充方法。

{"title":"A pH-responsive production of hydroxyl radical in Fenton process","authors":"Pengyi Wang , Fan Kang , Xiangbin Huang , Zhipeng Luo , Jing Zou , Min Yang , Meng Sun , Xin Yu , Huabin Zeng","doi":"10.1016/j.ese.2025.100566","DOIUrl":"10.1016/j.ese.2025.100566","url":null,"abstract":"<div><div>Efficient management of temporal latency and spatial heterogeneity remains a critical challenge in sensor-based pH regulation for smart water management, primarily due to inherent response delays and mass transfer constraints. In oxidation systems with dynamic pH environments, delayed responses can lead to issues such as cyanide release, unwanted side reactions, or pipe damage. To address these challenges, we propose a “pause-then-adjust” control strategy, exploiting the pH-responsive generation of hydroxyl radicals (<sup>•</sup>OH) in a modified Fenton reaction system. This system utilizes hydroxylamine as an electron donor and ethylenediaminetetraacetic acid (EDTA) as a stabilizer for iron ions. Within the pH range of 7.0–10.0, the coexistence of [Fe<sup>2+</sup>-EDTA]<sup>2−</sup> and [Fe<sup>3+</sup>-OH-EDTA]<sup>2−</sup> complexes facilitates efficient electron transfer, resulting in the selective and sustained production of <sup>•</sup>OH radicals. The inherent pH-responsiveness of this strategy enables rapid and spatially coherent adjustments, offering a robust supplementary method for addressing complex and evolving requirements in advanced water treatment systems.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100566"},"PeriodicalIF":14.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the potential of designed microbial consortia: A breakthrough for sustainable waste management and climate resilience 释放设计微生物群落的潜力：可持续废物管理和气候适应能力的突破

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-03-27 DOI: 10.1016/j.ese.2025.100558

Ginevra Giangeri , Stefano Campanaro , Nikos C. Kyrpides , Irini Angelidaki

引用次数: 0

Size matters: Anaerobic granules exhibit distinct ecological and physico-chemical gradients across biofilm size 大小问题：厌氧颗粒在生物膜大小上表现出不同的生态和物理化学梯度

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-03-27 DOI: 10.1016/j.ese.2025.100561

Anna Trego , Cristina Morabito , Isabelle Bourven , Giles Guibaud , Vincent O'Flaherty , Gavin Collins , Umer Zeeshan Ijaz

Anaerobic biological decomposition of organic matter is ubiquitous in Nature wherever anaerobic environments prevail, and is catalysed by hydrolytic, fermentative, acetogenic, methanogenic, and various other groups. It is also harnessed in innovative ways in engineered systems that may rely on small (0.1–4.0 mm), spherical, anaerobic granules. These biofilms are crucial to the operational success of a range of widely applied engineered-ecosystems designed for wastewater treatment. The structure and function of granule microbiomes underpin their utility. Here, granules were separated into ten size fractions (proxies for age), hypothesizing that small granules are ‘young’ and larger ones are ‘old’. Gradients were observed across size in terms of volatile solids, density, settleability, biofilm morphology, methanogenic activity, and profiles of extracellular polymeric substances, suggesting ongoing development of physico-chemical characteristics as granules develop. Short-read amplicon sequencing indicated a negative relationship between granule size and community diversity. Furthermore, as size increased, the methanogenic archaea dominated the microbiome. Small granules were found to harbour a sub-group of highly specific taxa, and the identification of generalists and specialists may point to substantial resilience of the microbiome. The findings of this study indicate opportunities for precision management of wastewater treatment systems. They suggest that size is an important indicator for aggregate utility – size may, indeed, determine many of the characteristics of both the individual-granule microbiomes and the overall function of a wastewater treatment system.

有机物的厌氧生物分解在自然界中无处不在，它由水解、发酵、产丙酮、产甲烷和其他各种基团催化。它还以创新的方式应用于工程系统中，这些系统可能依赖于小型（0.1-4.0毫米）球形厌氧颗粒。这些生物膜对于一系列广泛应用于污水处理的工程生态系统的操作成功至关重要。颗粒微生物组的结构和功能是其实用性的基础。在这里，颗粒被分成十个大小的分数（代表年龄），假设小颗粒是“年轻的”，大颗粒是“老的”。在挥发性固体、密度、可沉降性、生物膜形态、产甲烷活性和细胞外聚合物质的分布方面，观察到不同大小的梯度，表明随着颗粒的形成，物理化学特征正在不断发展。短读扩增子测序结果表明，颗粒大小与群落多样性呈负相关。此外，随着体型的增加，产甲烷的古菌在微生物群中占主导地位。小颗粒被发现含有高度特异性分类群的亚群，对通才和专才的识别可能表明微生物组具有实质性的弹性。这项研究的结果表明了废水处理系统精确管理的机会。他们认为，大小是总体效用的一个重要指标——实际上，大小可能决定了单个颗粒微生物群的许多特征和废水处理系统的整体功能。

{"title":"Size matters: Anaerobic granules exhibit distinct ecological and physico-chemical gradients across biofilm size","authors":"Anna Trego , Cristina Morabito , Isabelle Bourven , Giles Guibaud , Vincent O'Flaherty , Gavin Collins , Umer Zeeshan Ijaz","doi":"10.1016/j.ese.2025.100561","DOIUrl":"10.1016/j.ese.2025.100561","url":null,"abstract":"<div><div>Anaerobic biological decomposition of organic matter is ubiquitous in Nature wherever anaerobic environments prevail, and is catalysed by hydrolytic, fermentative, acetogenic, methanogenic, and various other groups. It is also harnessed in innovative ways in engineered systems that may rely on small (0.1–4.0 mm), spherical, anaerobic granules. These biofilms are crucial to the operational success of a range of widely applied engineered-ecosystems designed for wastewater treatment. The structure and function of granule microbiomes underpin their utility. Here, granules were separated into ten size fractions (proxies for age), hypothesizing that small granules are ‘young’ and larger ones are ‘old’. Gradients were observed across size in terms of volatile solids, density, settleability, biofilm morphology, methanogenic activity, and profiles of extracellular polymeric substances, suggesting ongoing development of physico-chemical characteristics as granules develop. Short-read amplicon sequencing indicated a negative relationship between granule size and community diversity. Furthermore, as size increased, the methanogenic archaea dominated the microbiome. Small granules were found to harbour a sub-group of highly specific taxa, and the identification of generalists and specialists may point to substantial resilience of the microbiome. The findings of this study indicate opportunities for precision management of wastewater treatment systems. They suggest that size is an important indicator for aggregate utility – size may, indeed, determine many of the characteristics of both the individual-granule microbiomes and the overall function of a wastewater treatment system.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100561"},"PeriodicalIF":14.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MnO2-Catalyzed electrocatalytic mineralization of triclosan in chlorinated wastewater 二氧化锰催化氯化废水中三氯生电催化矿化研究

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-03-26 DOI: 10.1016/j.ese.2025.100559

Asma Batool , Shan Shao , Kartick Chandra Majhi , Azeem Mushtaq , Yi Jiang , Wingkei Ho , Yiu Fai Tsang , Yuhe He , Kenneth Mei Yee Leung , Jason Chun-Ho Lam

The rising concentrations of xenobiotic aromatic compounds in the environment pose significant risks to human and ecosystem health. Developing a universal, environmentally benign, and scalable platform for mineralizing organic pollutants before their release into the environment is therefore crucial. Electrocatalysis can be highly advantageous for wastewater treatment because it is immediately responsive upon applying potential, requires no additional chemicals, and typically uses heterogeneous catalysts. However, achieving efficient electrochemical mineralization of wastewater pollutants at parts-per-million (ppm) levels remains a challenge. Here, we report the use of manganese dioxide (MnO₂), an Earth-abundant, chemically benign, and cost-effective electrocatalyst, to achieve over 99 % mineralization of triclosan (TCS) and other halogenated phenols at ppm levels. Two highly active MnO₂ phases—α-MnO₂-CC and δ-MnO₂-CC—were fabricated on inexpensive carbon cloth (CC) support and evaluated for their ability to oxidatively degrade TCS in pH-neutral conditions, including simulated chlorinated wastewater, real wastewater, and both synthetic and real landfill leachates. Total organic carbon analysis confirmed the effective degradation of TCS. Electron paramagnetic resonance and ultraviolet–visible spectroscopy identified reactive oxygen species, enabling the construction of a detailed TCS degradation pathway. Upon optimization, the TCS removal rate reached 38.38 nmol min⁻¹, surpassing previously reported rates achieved with precious and toxic metal co-catalysts. These findings highlight MnO₂-CC as a promising, eco-friendly electrocatalyst with strong potential for upscaled remediation of organic pollutants in wastewater treatment.

环境中外生芳香族化合物浓度的上升对人类和生态系统健康构成重大风险。因此，开发一种通用的、环保的、可扩展的平台，在有机污染物释放到环境之前将其矿化是至关重要的。电催化对废水处理非常有利，因为它对施加电位立即反应，不需要额外的化学物质，并且通常使用多相催化剂。然而，在百万分之一（ppm）的水平上实现废水污染物的有效电化学矿化仍然是一个挑战。在这里，我们报道了二氧化锰（MnO2）的使用，二氧化锰是一种地球上丰富的、化学良性的、具有成本效益的电催化剂，可以实现三氯生（TCS）和其他卤化酚在ppm水平下超过99%的矿化。在廉价碳布（CC）载体上制备了两种高活性MnO2相α-MnO2-CC和δ-MnO2-CC，并对其在ph中性条件下氧化降解TCS的能力进行了评价，包括模拟氯化废水、真实废水、合成和真实垃圾渗滤液。总有机碳分析证实了TCS的有效降解。电子顺磁共振和紫外可见光谱鉴定了活性氧，构建了详细的TCS降解途径。优化后，TCS去除率达到38.38 nmol min - 1，超过了之前报道的贵金属和有毒金属共催化剂的去除率。这些发现突出了MnO2-CC作为一种有前途的、环保的电催化剂，在污水处理中有机污染物的大规模修复方面具有强大的潜力。

{"title":"MnO2-Catalyzed electrocatalytic mineralization of triclosan in chlorinated wastewater","authors":"Asma Batool , Shan Shao , Kartick Chandra Majhi , Azeem Mushtaq , Yi Jiang , Wingkei Ho , Yiu Fai Tsang , Yuhe He , Kenneth Mei Yee Leung , Jason Chun-Ho Lam","doi":"10.1016/j.ese.2025.100559","DOIUrl":"10.1016/j.ese.2025.100559","url":null,"abstract":"<div><div>The rising concentrations of xenobiotic aromatic compounds in the environment pose significant risks to human and ecosystem health. Developing a universal, environmentally benign, and scalable platform for mineralizing organic pollutants before their release into the environment is therefore crucial. Electrocatalysis can be highly advantageous for wastewater treatment because it is immediately responsive upon applying potential, requires no additional chemicals, and typically uses heterogeneous catalysts. However, achieving efficient electrochemical mineralization of wastewater pollutants at parts-per-million (ppm) levels remains a challenge. Here, we report the use of manganese dioxide (MnO<sub>2</sub>), an Earth-abundant, chemically benign, and cost-effective electrocatalyst, to achieve over 99 % mineralization of triclosan (TCS) and other halogenated phenols at ppm levels. Two highly active MnO<sub>2</sub> phases—α-MnO<sub>2</sub>-CC and δ-MnO<sub>2</sub>-CC—were fabricated on inexpensive carbon cloth (CC) support and evaluated for their ability to oxidatively degrade TCS in pH-neutral conditions, including simulated chlorinated wastewater, real wastewater, and both synthetic and real landfill leachates. Total organic carbon analysis confirmed the effective degradation of TCS. Electron paramagnetic resonance and ultraviolet–visible spectroscopy identified reactive oxygen species, enabling the construction of a detailed TCS degradation pathway. Upon optimization, the TCS removal rate reached 38.38 nmol min<sup>−1</sup>, surpassing previously reported rates achieved with precious and toxic metal co-catalysts. These findings highlight MnO<sub>2</sub>-CC as a promising, eco-friendly electrocatalyst with strong potential for upscaled remediation of organic pollutants in wastewater treatment.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100559"},"PeriodicalIF":14.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-resolved metatranscriptomics unveils distinct microbial functionalities across aggregate sizes in aerobic granular sludge 基因组解析的超转录组学揭示了好氧颗粒污泥中不同粒径的微生物功能

IF 14 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Science and Ecotechnology

Pub Date : 2025-03-25 DOI: 10.1016/j.ese.2025.100560

A.Y.A. Mohamed , Laurence Gill , Alejandro Monleon , Mario Pronk , Mark van Loosdrecht , Pascal E. Saikaly , Muhammad Ali

Microbial aggregates of different sizes in aerobic granular sludge (AGS) systems have been shown to exhibit distinct microbial community compositions. However, studies comparing the microbial activities of different-sized aggregates in AGS systems remain limited. In this study, genome-resolved metatranscriptomics was used to investigate microbial activity patterns within differently sized aggregates in a full-scale AGS plant. Our analysis revealed a weak correlation between the relative abundance of metagenome-assembled genomes (MAGs) and their transcriptomic activity, indicating that microbial abundance does not directly correspond to metabolic activity within the system. Flocculent sludge (FL; <0.2 mm) predominantly featured active nitrifiers and fermentative polyphosphate-accumulating organisms (PAOs) from Candidatus Phosphoribacter, while small granules (SG; 0.2–1.0 mm) and large granules (LG; >1.0 mm) hosted more metabolically active PAOs affiliated with Ca. Accumulibacter. Differential gene expression analysis further supported these findings, demonstrating significantly higher expression levels of key phosphorus uptake genes associated with Ca. Accumulibacter in granular sludge (SG and LG) compared to flocculent sludge. Conversely, Ca. Phosphoribacter showed higher expression of these genes in the FL fraction. This study highlights distinct functional roles and metabolic activities of crucial microbial communities depending on aggregate size within AGS systems, offering new insights into optimizing wastewater treatment processes.

在好氧颗粒污泥（AGS）系统中，不同大小的微生物聚集体显示出不同的微生物群落组成。然而，比较AGS系统中不同粒径团聚体微生物活性的研究仍然有限。在这项研究中，基因组解析亚转录组学被用于研究全尺寸AGS植物中不同大小聚集体内的微生物活动模式。我们的分析显示，宏基因组组装基因组（MAGs）的相对丰度与其转录组活性之间存在弱相关性，这表明微生物丰度并不直接对应于系统内的代谢活性。絮凝污泥；0.2 mm)主要是活性硝化菌和来自Candidatus Phosphoribacter的发酵性聚磷微生物（PAOs），而小颗粒(SG；0.2-1.0 mm)和大颗粒(LG；>1.0 mm)含有更多与积累杆菌相关的代谢活性PAOs。差异基因表达分析进一步支持了这些发现，表明与絮凝污泥相比，颗粒污泥（SG和LG）中与Ca. Accumulibacter相关的关键磷吸收基因的表达水平显著更高。相反，Ca. Phosphoribacter在FL片段中表现出较高的这些基因表达。这项研究强调了AGS系统中关键微生物群落的不同功能作用和代谢活动，这取决于它们的聚集大小，为优化废水处理工艺提供了新的见解。

{"title":"Genome-resolved metatranscriptomics unveils distinct microbial functionalities across aggregate sizes in aerobic granular sludge","authors":"A.Y.A. Mohamed , Laurence Gill , Alejandro Monleon , Mario Pronk , Mark van Loosdrecht , Pascal E. Saikaly , Muhammad Ali","doi":"10.1016/j.ese.2025.100560","DOIUrl":"10.1016/j.ese.2025.100560","url":null,"abstract":"<div><div>Microbial aggregates of different sizes in aerobic granular sludge (AGS) systems have been shown to exhibit distinct microbial community compositions. However, studies comparing the microbial activities of different-sized aggregates in AGS systems remain limited. In this study, genome-resolved metatranscriptomics was used to investigate microbial activity patterns within differently sized aggregates in a full-scale AGS plant. Our analysis revealed a weak correlation between the relative abundance of metagenome-assembled genomes (MAGs) and their transcriptomic activity, indicating that microbial abundance does not directly correspond to metabolic activity within the system. Flocculent sludge (FL; <0.2 mm) predominantly featured active nitrifiers and fermentative polyphosphate-accumulating organisms (PAOs) from <em>Candidatus</em> Phosphoribacter, while small granules (SG; 0.2–1.0 mm) and large granules (LG; >1.0 mm) hosted more metabolically active PAOs affiliated with <em>Ca.</em> Accumulibacter. Differential gene expression analysis further supported these findings, demonstrating significantly higher expression levels of key phosphorus uptake genes associated with <em>Ca.</em> Accumulibacter in granular sludge (SG and LG) compared to flocculent sludge. Conversely, <em>Ca.</em> Phosphoribacter showed higher expression of these genes in the FL fraction. This study highlights distinct functional roles and metabolic activities of crucial microbial communities depending on aggregate size within AGS systems, offering new insights into optimizing wastewater treatment processes.</div></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"25 ","pages":"Article 100560"},"PeriodicalIF":14.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0