IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech

Pub Date : 2024-07-22 DOI: 10.3390/biotech13030027

Prabhaharan Renganathan, Edgar Omar Rueda Puente, Natalia V Sukhanova, Lira A Gaysina

The global population is expected to reach 9.5 billion, which means that crop productivity needs to double to meet the growing population's food demand. Soil degradation and environmental factors, such as climate events, significantly threaten crop production and global food security. Furthermore, rapid urbanization has led to 55% of the world's population migrating to cities, and this proportion is expected to increase to 75% by 2050, which presents significant challenges in producing staple foods through conventional hinterland farming. Numerous studies have proposed various sustainable farming techniques to combat the shortage of farmable land and increase food security in urban areas. Soilless farming techniques such as hydroponics have gained worldwide popularity due to their resource efficiency and production of superior-quality fresh products. However, using chemical nutrients in a conventional hydroponic system can have significant environmental impacts, including eutrophication and resource depletion. Incorporating microalgae into hydroponic systems as biostimulants offers a sustainable and ecofriendly approach toward circular bioeconomy strategies. The present review summarizes the plant growth-promoting activity of microalgae as biostimulants and their mechanisms of action. We discuss their effects on plant growth parameters under different applications, emphasizing the significance of integrating microalgae into a closed-loop circular economy model to sustainably meet global food demands.

全球人口预计将达到 95 亿，这意味着作物生产率需要翻一番才能满足人口不断增长的粮食需求。土壤退化和气候事件等环境因素严重威胁着作物生产和全球粮食安全。此外，快速城市化导致全球 55% 的人口迁移到城市，预计到 2050 年这一比例将增至 75%，这给通过传统腹地耕作生产主食带来了巨大挑战。许多研究提出了各种可持续耕作技术，以解决可耕地短缺问题，提高城市地区的粮食安全。水耕法等无土栽培技术因其资源效率高、可生产优质新鲜产品而在全球范围内广受欢迎。然而，在传统的水培系统中使用化学营养物质会对环境造成严重影响，包括富营养化和资源枯竭。在水培系统中加入微藻作为生物刺激剂，为实现循环生物经济战略提供了一种可持续和生态友好的方法。本综述总结了微藻作为生物刺激剂对植物生长的促进作用及其作用机制。我们讨论了它们在不同应用条件下对植物生长参数的影响，强调了将微藻纳入闭环循环经济模式以可持续地满足全球粮食需求的重要意义。

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引用次数: 0

Strategies for Increasing the Throughput of Genetic Screening: Lessons Learned from the COVID-19 Pandemic within a University Community 提高基因筛查通量的策略：从大学社区 COVID-19 大流行中汲取的经验教训

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech

Pub Date : 2024-07-11 DOI: 10.3390/biotech13030026

Fernanda Miguel, A. R. Baleizão, A. G. Gomes, Helena Caria, Fátima N. Serralha, Marta C. Justino

Amidst the COVID-19 pandemic, the Polytechnic University of Setúbal (IPS) used its expertise in molecular genetics to establish a COVID-19 laboratory, addressing the demand for community-wide testing. Following standard protocols, the IPS COVID Lab received national accreditation in October 2020 and was registered in February 2021. With the emergence of new SARS-CoV-2 variants and safety concerns for students and staff, the lab was further challenged to develop rapid and sensitive diagnostic technologies. Methodologies such as sample-pooling extraction and multiplex protocols were developed to enhance testing efficiency without compromising accuracy. Through Real-Time Reverse Transcription Polymerase Chain Reaction (RT-qPCR) analysis, the effectiveness of sample pooling was validated, proving to be a clear success in COVID-19 screening. Regarding multiplex analysis, the IPS COVID Lab developed an in-house protocol, achieving a sensitivity comparable to that of standard methods while reducing operational time and reagent consumption. This approach, requiring only two wells of a PCR plate (instead of three for samples), presents a more efficient alternative for future testing scenarios, increasing its throughput and testing capacity while upholding accuracy standards. The lessons learned during the SARS-CoV-2 pandemic provide added value for future pandemic situations.

在 COVID-19 大流行期间，塞图巴尔理工大学（IPS）利用其在分子遗传学方面的专业知识建立了 COVID-19 实验室，以满足全社区的检测需求。按照标准协议，IPS COVID 实验室于 2020 年 10 月获得国家认证，并于 2021 年 2 月注册。随着 SARS-CoV-2 新变种的出现以及对学生和教职员工安全的担忧，实验室面临着开发快速、灵敏诊断技术的进一步挑战。为了在不影响准确性的前提下提高检测效率，实验室开发了样本池提取和多重方案等方法。通过实时反转录聚合酶链反应（RT-qPCR）分析，验证了样本池提取的有效性，证明在 COVID-19 筛查中取得了明显的成功。在多重分析方面，IPS COVID 实验室开发了一种内部方案，实现了与标准方法相当的灵敏度，同时减少了操作时间和试剂消耗。这种方法只需要 PCR 板的两个孔（而不是样品的三个孔），为今后的检测方案提供了一个更有效的选择，在坚持准确性标准的同时提高了吞吐量和检测能力。在 SARS-CoV-2 大流行期间吸取的经验教训为未来的大流行情况提供了附加值。

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引用次数: 0

In Vitro: The Extraordinary Enhancement in Rutin Accumulation and Antioxidant Activity in Philodendron “Imperial Red” Plantlets Using Ti-Mo-Ni-O Nanotubes as a Novel Elicitor 体外：使用新型诱导剂 Ti-Mo-Ni-O 纳米管非凡地提高了菲洛德龙 "帝王红 "小植株的芦丁积累和抗氧化活性

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech

Pub Date : 2024-07-04 DOI: 10.3390/biotech13030024

Hanan S. Ebrahim, Nourhan M. Deyab, B. Shaheen, A. Gabr, Nageh K. Allam

Rutin, a flavonoid phytochemical compound, plays a vital role in human health. It is used in treating capillary fragility and has anti-Alzheimer, anti-inflammatory, and antioxidant effects. In this study, Ti-Mo-Ni-O nanotubes (NTs) were used, for the first time, in an unprecedented plant biotechnology application, wherein in vitro Philodendron shoots (Philodendron erubescens) known as “Imperial Red” were targeted for rutin accumulation. The antioxidant responses and the accumulation of rutin were evaluated in treated Philodendron erubescens (P. erubescens) shoots using 5.0 mg/L of Ti-Mo-Ni-O NTs. The total phenolic content and total flavonoid content were estimated, and an ABTS+ assay, FRAP assay, and iron metal chelation assay were performed. The application of Ti-Mo-Ni-O NTs enhanced the rutin content considerably from 0.02 mg/g to 2.96 mg/g for dry-weight shootlet extracts. Therefore, the use of Ti-Mo-Ni-O NTs is proposed to be a superior alternative to elevate the rutin content. The aim of the current study is to target P. erubescens shoots grown in vitro for the accumulation of rutin compounds using Ti-Mo-Ni-O NT powder, to determine the quantitative and qualitative accumulation of rutin via HPLC–DAD analysis, and to estimate the antioxidant activity of P. erubescens shoot extract. This study presents a novel methodology for utilizing nano-biotechnology in the synthesis of plant secondary metabolites.

芦丁是一种黄酮类植物化学化合物，对人体健康起着至关重要的作用。它可用于治疗毛细血管脆性，并具有抗老年痴呆、抗炎和抗氧化作用。在这项研究中，Ti-Mo-Ni-O 纳米管（NTs）首次被用于一种前所未有的植物生物技术应用中，体外培植的被称为 "帝王红 "的菲洛德龙（Philodendron erubescens）嫩枝成为芦丁积累的目标。使用 5.0 毫克/升的钛-钼-镍-氧化物（Ti-Mo-Ni-O NTs）对处理过的飞燕草（Philodendron erubescens）嫩枝的抗氧化反应和芦丁积累情况进行了评估。评估了总酚含量和总黄酮含量，并进行了 ABTS+ 试验、FRAP 试验和铁金属螯合试验。应用 Ti-Mo-Ni-O NTs 后，小芽干重提取物中的芦丁含量从 0.02 毫克/克大幅提高到 2.96 毫克/克。因此，使用钛-钼-镍-氧化物催化还原剂是提高芦丁含量的一种优选方法。本研究的目的是利用 Ti-Mo-Ni-O NT 粉末针对体外生长的欧鼠李嫩枝进行芦丁化合物的积累，通过 HPLC-DAD 分析确定芦丁的定量和定性积累，并评估欧鼠李嫩枝提取物的抗氧化活性。本研究提出了一种利用纳米生物技术合成植物次生代谢物的新方法。

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引用次数: 0

An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green 从蓖麻变种下胚轴幼年胼胝体进行体芽器官发生的高效快速方法桑给巴尔绿

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech

Pub Date : 2024-07-04 DOI: 10.3390/biotech13030025

Danaya V. Demidenko, N. V. Varlamova, Taisiya M. Soboleva, A. V. Shitikova, M. Khaliluev

Aseptic seedlings of different ages derived from surface-sterilized mature seeds were applied as an explant source. Various explants such as 7- and 21-day-old hypocotyl fragments, 42-day-old nodal stem segments, and transverse nodal segments of stem, as well as leaf petioles, were cultured on the agar-solidified Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/L IAA, 5 mg/L AgNO3 and different types and concentrations of cytokinin (1 mg/L zeatin, 0.25 mg/L thidiazuron (TDZ), and 5 mg/L 6-benzylaminopurine (6-BAP)). Consequently, it was found that 7- and 21-day-old hypocotyl fragments, as well as nodal stem segments obtained from adult aseptic seedlings, are characterized by a high explant viability and callus formation capacity with a frequency of 79.7–100%. However, the success of in vitro somatic shoot organogenesis was significantly determined not only by the culture medium composition and explant type but also depending on its age, as well as on the size and explant preparation in cases of hypocotyl and age-matched nodal stem fragments, respectively. Multiple somatic shoot organogenesis (5.7 regenerants per explant) with a frequency of 67.5% was achieved during 3 subcultures of juvenile hypocotyl-derived callus tissue on MS culture medium containing 0.25 mg/L TDZ as cytokinin source. Castor bean regenerants were excised from the callus and successfully rooted on ½ MS basal medium without exogenous auxin (81%). In vitro plantlets with well-developed roots were adapted to ex vitro conditions with a frequency of 90%.

从表面灭菌的成熟种子中提取不同年龄的无菌幼苗作为外植体来源。各种外植体，如 7 天和 21 天的下胚轴片段、42 天的茎节段、茎的横节段以及叶柄，在琼脂固化的 Murashige 和 Skoog（MS）基础培养基上培养，培养基中添加 0.1 mg/L IAA、5 mg/L AgNO3 以及不同种类和浓度的细胞分裂素（1 mg/L玉米素、0.25 mg/L 噻虫嗪（TDZ）和 5 mg/L 6-苄氨基嘌呤（6-BAP））。结果发现，7 天和 21 天的下胚轴片段以及从无菌成苗上获得的节茎片段具有较高的外植体活力和形成胼胝体的能力，频率为 79.7%-100%。然而，体外体细胞芽器官发生的成功与否不仅取决于培养基成分和外植体类型，还取决于外植体的年龄，以及下胚轴和与年龄匹配的节茎片段的大小和外植体制备。在含有 0.25 毫克/升 TDZ 作为细胞分裂素源的 MS 培养基上对幼年下胚轴衍生的胼胝体组织进行 3 次移栽，实现了 67.5%的多体芽器官发生（每个外植体 5.7 个再生体）。从胼胝体中提取的蓖麻再生植株在不含外源辅助素的 ½ MS 基础培养基上成功生根（81%）。根系发达的离体植株适应离体条件的频率为 90%。

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引用次数: 0

Systemic Effects of Photoactivated 5,10,15,20-tetrakis(N-methylpyridinium-3-yl) Porphyrin on Healthy Drosophila melanogaster 光活化 5,10,15,20-四(N-甲基吡啶-3-基)卟啉对健康黑腹果蝇的系统影响

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech

Pub Date : 2024-07-03 DOI: 10.3390/biotech13030023

Ana Filošević Vujnović, Sara Čabrijan, Martina Mušković, Nela Malatesti, Rozi Andretić Waldowski

Porphyrins are frequently employed in photodynamic therapy (PDT), a non-invasive technique primarily utilized to treat subcutaneous cancers, as photosensitizing agents (PAs). The development of a new PA with improved tissue selectivity and efficacy is crucial for expanding the application of PDT for the management of diverse cancers. We investigated the systemic effects of 5,10,15,20-tetrakis(N-methylpyridinium-3-yl)-porphyrin (TMPyP3) using Drosophila melanogaster adult males. We established the oral administration schedule and demonstrated that TMPyP3 was absorbed and stored higher in neuronal than in non-neuronal extracts. Twenty-four hours after oral TMPyP3 photoactivation, the quantity of hydrogen peroxide (H2O2) increased, but exclusively in the head extracts. Regardless of photoactivation, TMPyP3 resulted in a reduced concentration of H2O2 after 7 days, and this was linked with a decreased capacity to climb, as indicated by negative geotaxis. The findings imply that systemic TMPyP3 therapy may disrupt redox regulation, impairing cellular signaling and behavioral outcomes in the process. To determine the disruptive effect of porphyrins on redox homeostasis, its duration, and the mechanistic variations in retention across various tissues, more research is required.

光动力疗法（PDT）是一种主要用于治疗皮下癌症的非侵入性技术，卟啉作为光敏剂（PAs）经常被用于该疗法中。开发一种具有更好的组织选择性和疗效的新型光敏剂对于扩大光动力疗法在治疗各种癌症中的应用至关重要。我们利用黑腹果蝇成年雄性研究了 5,10,15,20-四（N-甲基吡啶-3-基）-卟啉（TMPyP3）的全身效应。我们确定了口服给药时间表，并证明神经元提取物对 TMPyP3 的吸收和储存量高于非神经元提取物。口服 TMPyP3 光活化 24 小时后，过氧化氢（H2O2）的数量增加，但仅限于头部提取物。无论光激活与否，TMPyP3 都会在 7 天后导致 H2O2 浓度降低，这与爬行能力下降有关，负向地心引力就表明了这一点。研究结果表明，全身性 TMPyP3 治疗可能会破坏氧化还原调节，在此过程中损害细胞信号传导和行为结果。要确定卟啉对氧化还原平衡的破坏作用、持续时间以及在不同组织中保留的机理变化，还需要进行更多的研究。

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引用次数: 0

A Machine Learning-Based Web Tool for the Severity Prediction of COVID-19 基于机器学习的 COVID-19 严重性预测网络工具

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech

Pub Date : 2024-07-01 DOI: 10.3390/biotech13030022

Avgi Christodoulou, Martha-Spyridoula Katsarou, Christina Emmanouil, Marios Gavrielatos, Dimitrios Georgiou, Annia Tsolakou, M. Papasavva, Vasiliki V Economou, Vasiliki Nanou, Ioannis Nikolopoulos, Maria Daganou, A. Argyraki, Evaggelos Stefanidis, Gerasimos Metaxas, E. Panagiotou, Ioannis Michalopoulos, N. Drakoulis

Predictive tools provide a unique opportunity to explain the observed differences in outcome between patients of the COVID-19 pandemic. The aim of this study was to associate individual demographic and clinical characteristics with disease severity in COVID-19 patients and to highlight the importance of machine learning (ML) in disease prognosis. The study enrolled 344 unvaccinated patients with confirmed SARS-CoV-2 infection. Data collected by integrating questionnaires and medical records were imported into various classification machine learning algorithms, and the algorithm and the hyperparameters with the greatest predictive ability were selected for use in a disease outcome prediction web tool. Of 111 independent features, age, sex, hypertension, obesity, and cancer comorbidity were found to be associated with severe COVID-19. Our prognostic tool can contribute to a successful therapeutic approach via personalized treatment. Although at the present time vaccination is not considered mandatory, this algorithm could encourage vulnerable groups to be vaccinated.

预测工具为解释所观察到的 COVID-19 大流行患者之间的预后差异提供了一个独特的机会。本研究旨在将 COVID-19 患者的个人人口统计学和临床特征与疾病严重程度联系起来，并强调机器学习 (ML) 在疾病预后中的重要性。该研究招募了 344 名确诊感染 SARS-CoV-2 且未接种疫苗的患者。通过整合问卷调查和医疗记录收集的数据被导入各种分类机器学习算法，然后选出预测能力最强的算法和超参数，用于疾病结果预测网络工具。在 111 个独立特征中，我们发现年龄、性别、高血压、肥胖和癌症合并症与严重 COVID-19 相关。我们的预后工具有助于通过个性化治疗成功地找到治疗方法。虽然目前疫苗接种还不是强制性的，但这种算法可以鼓励弱势群体接种疫苗。

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引用次数: 0

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria. 关键参数对从耐热蓝藻中提取浓缩 C-PE 的影响

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BioTech

Pub Date : 2024-06-24 DOI: 10.3390/biotech13030021

Ariadna H Vergel-Suarez, Janet B García-Martínez, German L López-Barrera, Néstor A Urbina-Suarez, Andrés F Barajas-Solano

This work aimed to identify the influence of pH, molarity, w/v fraction, extraction time, agitation, and either a sodium (Na₂HPO₄·7H₂O-NaH₂PO₄·H₂O) or potassium buffer (K₂HPO₄-KH₂PO₄) used in the extraction of C-phycoerythrin (C-PE) from a thermotolerant strain of Potamosiphon sp. An experimental design (Minimum Run Resolution V Factorial Design) and a Central Composite Design (CCD) were used. According to the statistical results of the first design, the K-PO₄ buffer, pH, molarity, and w/v fraction are vital factors that enhance the extractability of C-PE. The construction of a CCD design of the experiments suggests that the potassium phosphate buffer at pH 5.8, longer extraction times (50 min), and minimal extraction speed (1000 rpm) are ideal for maximizing C-PE concentration, while purity is unaffected by the design conditions. This optimization improves extraction yields and maintains the desired bright purple color of the phycobiliprotein.

本研究旨在确定pH值、摩尔浓度、w/v分数、萃取时间、搅拌以及钠缓冲液（Na2HPO4-7H2O-NaH2PO4-H2O）或钾缓冲液（K2HPO4-KH2PO4）对从耐热菌株Potamosiphon sp.中萃取C-PE的影响。本研究采用了实验设计（最小运行分辨率V因子设计）和中央综合设计（CCD）。根据第一种设计的统计结果，K-PO4 缓冲液、pH 值、摩尔浓度和 w/v 分数是提高 C-PE 萃取率的关键因素。实验的 CCD 设计表明，pH 值为 5.8 的磷酸二氢钾缓冲液、较长的萃取时间（50 分钟）和最低的萃取速度（1000 转/分钟）是最大化 C-PE 浓度的理想条件，而纯度则不受设计条件的影响。这种优化方法提高了提取率，并保持了所需的亮紫色藻胆蛋白。

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引用次数: 0

Enhancing Bovine Embryo Development In Vitro Using Oil-in-Water Nanoemulsions as Specific Carriers for Essential Lipids 利用水包油型纳米乳液作为必需脂质的特异性载体促进牛胚胎体外发育

Q2 Biochemistry, Genetics and Molecular Biology

BioTech

Pub Date : 2024-06-11 DOI: 10.3390/biotech13020019

Daniel Enrique López Angulo, R. Lourenço, A. Bridi, M. A. Chaves, J. D. da Silveira, P. J. Sobral

Worldwide meat consumption and production have nearly quintupled in the last 60 years. In this context, research and the application of new technologies related to animal reproduction have evolved in an accelerated way. The objective of the present study was to apply nanoemulsions (NEs) as carriers of lipids to feed bovine embryos in culture media and verify their impact on the development of embryos produced in vitro. The NEs were characterized by particle size, polydispersity, size distribution, physical stability, morphology using atomic force microscopy (AFM), surface tension, density, pH, and rheological behavior. The NEs were prepared by the emulsification/evaporation technique. A central composite rotatable design (CCRD) was used to optimize the NE fabrication parameters. The three optimized formulations used in the embryo application showed an emulsion stability index (ESI) between 0.046 and 0.086, which reflects high stability. The mean droplet diameter analyzed by laser diffraction was approximately 70–80 nm, suggesting a possible transit across the embryonic zona pellucida with pores of an average 90 nm in diameter. AFM images clearly confirm the morphology of spherical droplets with a mean droplet diameter of less than 100 nm. The optimized formulations added during the higher embryonic genome activation phase in bovine embryos enhanced early embryonic development.

在过去的 60 年中，全球肉类消费和生产几乎翻了五番。在这种情况下，与动物繁殖相关的新技术的研究和应用也在加速发展。本研究的目的是应用纳米乳液（NEs）作为脂质载体，在培养基中喂养牛胚胎，并验证其对体外生产的胚胎发育的影响。纳米乳液的特征包括粒度、多分散性、粒度分布、物理稳定性、使用原子力显微镜（AFM）观察的形态、表面张力、密度、pH 值和流变行为。NEs 采用乳化/蒸发技术制备。采用中心复合可旋转设计（CCRD）来优化 NE 的制造参数。胚胎应用中使用的三种优化配方的乳液稳定性指数（ESI）介于 0.046 和 0.086 之间，体现了较高的稳定性。通过激光衍射分析，液滴的平均直径约为 70-80 nm，这表明液滴有可能穿过胚胎透明带，其孔隙的平均直径为 90 nm。原子力显微镜图像清楚地证实了平均液滴直径小于 100 纳米的球形液滴形态。在牛胚胎较高的胚胎基因组激活阶段添加的优化配方可促进早期胚胎发育。

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引用次数: 0

Spectrofluorimetric Analysis of Riboflavin Content during Kombucha Fermentation 昆布茶发酵过程中核黄素含量的荧光光谱分析

Q2 Biochemistry, Genetics and Molecular Biology

BioTech

Pub Date : 2024-06-11 DOI: 10.3390/biotech13020020

M. Čakić Semenčić, A. Biedrzycka, Anna Kiczor, S. Beluhan, F. Šupljika

Kombucha is a traditional beverage obtained by the microbial fermentation of tea using a symbiotic culture of bacteria and yeasts. In addition to several documented functional properties, such as anti-inflammatory activity and antioxidant activity, kombucha is often credited with high levels of vitamins, including riboflavin. To our knowledge, the vitamin B2 content in traditionally prepared kombucha has been determined in only two studies, in which the concentration measured by the HPLC technique ranged from 2.2 × 10−7 to 2.1 × 10−4 mol dm−3. These unexplained differences of three orders of magnitude in the vitamin B2 content prompted us to determine its concentration during the cultivation of kombucha under very similar conditions by spectrofluorimetry. The B2 concentrations during the 10-day fermentation of black tea ranged from 7.6 × 10−8 to 3.3 × 10−7 mol dm−3.

昆布茶是一种传统饮料，通过细菌和酵母菌的共生培养，对茶叶进行微生物发酵而制成。除了抗炎和抗氧化等几种有记载的功能特性外，昆布茶还经常被认为含有大量维生素，包括核黄素。据我们所知，只有两项研究测定了传统制作的昆布茶中的维生素 B2 含量，其中采用 HPLC 技术测定的浓度范围为 2.2 × 10-7 至 2.1 × 10-4 mol dm-3。这些无法解释的维生素 B2 含量三个数量级的差异，促使我们在非常相似的条件下，通过分光荧光测定法测定昆布茶培养过程中的维生素 B2 浓度。在红茶 10 天的发酵过程中，维生素 B2 的浓度范围为 7.6 × 10-8 至 3.3 × 10-7 mol dm-3。

引用次数: 0

Phaseolus coccineus L. Landraces in Greece: Microsatellite Genotyping and Molecular Characterization for Landrace Authenticity and Discrimination 希腊的 Phaseolus coccineus L. 陆种：微卫星基因分型和分子特征描述促进陆地品系的真实性和鉴别性

Q2 Biochemistry, Genetics and Molecular Biology

BioTech

Pub Date : 2024-06-07 DOI: 10.3390/biotech13020018

Irene Bosmali, G. Lagiotis, Ioannis Ganopoulos, Eleni Stefanidou, P. Madesis, C. Biliaderis

Phaseolus coccineus L. is a highly valuable crop for human consumption with a high protein content and other associated health benefits. Herein, 14 P. coccineus L. landraces were selected for genetic characterization: two Protected Geographical Indication (PGI) landraces from the Prespon area, namely “Gigantes” (“G”) and “Elephantes” (“E”), and 12 additional landraces from the Greek Gene Bank collection of beans (PC1–PC12). The genetic diversity among these landraces was assessed using capillary electrophoresis utilizing fluorescence-labeled Simple Sequence Repeat (SSR) and Expressed Sequence Tag (EST); Simple Sequence Repeat (SSR) is a molecular marker technology. The “G” and “E” Prespon landraces were clearly distinguished among them, as well as from the PC1 to PC12 landraces, indicating the unique genetic identity of the Prespon beans. Overall, the genetic characterization of the abundant Greek bean germplasm using molecular markers can aid in the genetic identification of “G” and “E” Prespon beans, thus preventing any form of fraudulent practices as well as supporting traceability management strategies for the identification of authenticity, and protection of the origin of local certified products.

可可豆（Phaseolus coccineus L.）是一种非常有价值的供人类食用的作物，具有高蛋白含量和其他相关的健康益处。在此，我们选择了 14 个 P. coccineus L. 陆生品系进行遗传特性分析：两个来自 Prespon 地区的受保护地理标志（PGI）陆生品系，即 "Gigantes"（"G"）和 "Elephantes"（"E"），以及另外 12 个来自希腊基因库收集的豆类陆生品系（PC1-PC12）。利用荧光标记的简单序列重复（SSR）和表达式序列标签（EST）进行毛细管电泳，评估了这些品种的遗传多样性；简单序列重复（SSR）是一种分子标记技术。结果表明，"G "和 "E "Prespon 陆生品系之间以及与 PC1 至 PC12 陆生品系之间有明显的区别，这表明 Prespon 大豆具有独特的遗传特性。总之，利用分子标记对丰富的希腊豆类种质进行遗传特征描述有助于对 "G "和 "E "Prespon 豆类进行遗传鉴定，从而防止任何形式的欺诈行为，并支持用于鉴定真伪和保护本地认证产品原产地的可追溯性管理策略。

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引用次数: 0

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