Sareh Bagheri, F. Firoozeh, elahe sasani, Tayebe Shahbazi, rezavan moniri
{"title":"Quinolone-Resistant Isolates of Acinetobacter baumannii in a Teaching Hospital in Iran","authors":"Sareh Bagheri, F. Firoozeh, elahe sasani, Tayebe Shahbazi, rezavan moniri","doi":"10.61186/iem.9.2.107","DOIUrl":"https://doi.org/10.61186/iem.9.2.107","url":null,"abstract":"","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139352572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Adekola, David Ojo, Saka Balogun, M. Dipeolu, Musa Mohammed, Adeolu Amusan
non-specific
泛
{"title":"Seroprevalence of Zika Virus IgM Antibodies in Pregnant Woman in Nigeria","authors":"H. Adekola, David Ojo, Saka Balogun, M. Dipeolu, Musa Mohammed, Adeolu Amusan","doi":"10.61186/iem.9.2.179","DOIUrl":"https://doi.org/10.61186/iem.9.2.179","url":null,"abstract":"non-specific","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139352933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Araz Majnooni, K. Ghazvini, A. Azimian, Saeed Amel-Jamehdar
were not typable. Only two sequence types were detected among 34 typeable isolates: 25 (73.5%) isolates belonged to ST80, and nine (26.5%) samples belonged to ST4. Conclusion : The high prevalence of ST4 and ST80 in most symptomatic infertile patients may be attributed to the higher pathogenicity of these types in the urogenital tract. However, our sample size was insufficient to draw such a conclusion., Further research on the prevention and treatment of Chlamydial infections could potentially help to reduce infertility in Iran.
{"title":"Prevalence of Chlamydia trachomatis Sequence Types 4 and 80 in Infertile Couples in North Khorasan, Iran","authors":"Araz Majnooni, K. Ghazvini, A. Azimian, Saeed Amel-Jamehdar","doi":"10.61186/iem.9.2.127","DOIUrl":"https://doi.org/10.61186/iem.9.2.127","url":null,"abstract":"were not typable. Only two sequence types were detected among 34 typeable isolates: 25 (73.5%) isolates belonged to ST80, and nine (26.5%) samples belonged to ST4. Conclusion : The high prevalence of ST4 and ST80 in most symptomatic infertile patients may be attributed to the higher pathogenicity of these types in the urogenital tract. However, our sample size was insufficient to draw such a conclusion., Further research on the prevention and treatment of Chlamydial infections could potentially help to reduce infertility in Iran.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139352604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : This study aims to investigate the in-vitro antibacterial activity, mineral and vitamin compositions, proximate composition, and organoleptic properties of a syrup derived from Vitex doniana fruits. Materials & Methods : V . doniana fruits were mashed, mixed with water, strained, and boiled to thicken the filtrate . The syrup’s antibacterial activity was tested on 7 clinical and 6 American Type Culture Collection (ATCC) isolates using well-in-agar diffusion method on Mueller Hinton agar. The fruit juice underwent mineral analysis using atomic spectroscopy and mass spectrometry. Proximate composition, vitamin, and organoleptic properties of a syrup were evaluated. Findings : Clinical Escherichia coli 0157:
{"title":"Inhibition of Bacterial growth, mineral, proximate and vitamin contents of a syrup prepared from Vitex doniana fruits","authors":"Fred Akharaiyi, Muniratu Maliki","doi":"10.61186/iem.9.2.137","DOIUrl":"https://doi.org/10.61186/iem.9.2.137","url":null,"abstract":"Background : This study aims to investigate the in-vitro antibacterial activity, mineral and vitamin compositions, proximate composition, and organoleptic properties of a syrup derived from Vitex doniana fruits. Materials & Methods : V . doniana fruits were mashed, mixed with water, strained, and boiled to thicken the filtrate . The syrup’s antibacterial activity was tested on 7 clinical and 6 American Type Culture Collection (ATCC) isolates using well-in-agar diffusion method on Mueller Hinton agar. The fruit juice underwent mineral analysis using atomic spectroscopy and mass spectrometry. Proximate composition, vitamin, and organoleptic properties of a syrup were evaluated. Findings : Clinical Escherichia coli 0157:","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139352750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reyhaneh Amiri, Majid Alipour, Ali Khajir Engasi, Ali Reza Amiri, Ramin Mofarrah
Background: Bacterial urinary tract infections are observed in all age groups due to the development of antibiotic-resistant species. This study aimed to investigate resistance genes gyrase subunit A ( gyrA) , topoisomerase IV ( parC ) subunit gene, beta lactamase ( blaZ ), erythromycin ribosome methylase ( ermC ), ermB , and ermA in Staphylococcus saporophyticus isolated from patients with urinary tract infections (UTIs) in Mazandaran Province, Iran. Materials & Methods: In this cross-sectional descriptive study, 3280 clinical samples were collected from patients with UTIs in Mazandaran Province from April to December 2022. Isolates were identified by biochemical tests. Microbial sensitivity tests were performed by disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Polymerase chain reaction (PCR) was used to check the presence of resistance genes. Findings: Out of a total of 3280 clinical samples collected, 2088 samples were detected by biochemical tests at the genus level
{"title":"Monitoring and Investigation of Resistance Genes gyrA, parC, blaZ, ermA, ermB, and ermC in Staphylococcus saprophyticus Isolated from Urinary Tract Infections in Mazandaran Province","authors":"Reyhaneh Amiri, Majid Alipour, Ali Khajir Engasi, Ali Reza Amiri, Ramin Mofarrah","doi":"10.61186/iem.9.2.117","DOIUrl":"https://doi.org/10.61186/iem.9.2.117","url":null,"abstract":"Background: Bacterial urinary tract infections are observed in all age groups due to the development of antibiotic-resistant species. This study aimed to investigate resistance genes gyrase subunit A ( gyrA) , topoisomerase IV ( parC ) subunit gene, beta lactamase ( blaZ ), erythromycin ribosome methylase ( ermC ), ermB , and ermA in Staphylococcus saporophyticus isolated from patients with urinary tract infections (UTIs) in Mazandaran Province, Iran. Materials & Methods: In this cross-sectional descriptive study, 3280 clinical samples were collected from patients with UTIs in Mazandaran Province from April to December 2022. Isolates were identified by biochemical tests. Microbial sensitivity tests were performed by disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Polymerase chain reaction (PCR) was used to check the presence of resistance genes. Findings: Out of a total of 3280 clinical samples collected, 2088 samples were detected by biochemical tests at the genus level","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139352404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shaghayegh Hemat jouy, farzan Lotfi, Rahim Akrami, jafar shahraki, R. Sheervalilou, Omolbanin Shahraki, H. Ghaznavi
Backgrounds: The primary goal of this study was to identify the potential association between COVID-19 prognosis and demographic and clinical features, underlying diseases, and drug and supplement use in patients admitted to Amir al-Momenin hospital in Zabol. Materials & Methods: This retrospective study surveyed the electronic health records of 848 COVID-19 patients hospitalized in a tertiary referral hospital in southeastern Iran from the beginning of the COVID-19 outbreak until the end of February 2021. Univariate and multiple analytical tests including unconditional and penalized logistic regressions were used for statistical analysis. Findings: Out of a total of 848 patients, 371 (43.75%) patients were female, and 477 (56.25%) patients were male. Age, underlying pulmonary and cardiovascular diseases, and loss of consciousness predicted a higher mortality rate. On the contrary, a negative chest X-ray was associated with a lower risk of death. Conclusion: Identifying predisposing factors of mortality in COVID-19 patients will help physicians provide more intensive care to those at higher risk of death by classifying patients based on risk factors and underlying diseases.
{"title":"Investigating the Association between COVID-19 Prognosis and Demographic and Clinical Features, Underlying Diseases, and Drug and Supplement Use in Patients Hospitalized in Zabol, Iran: A Single-Center Retrospective Study","authors":"Shaghayegh Hemat jouy, farzan Lotfi, Rahim Akrami, jafar shahraki, R. Sheervalilou, Omolbanin Shahraki, H. Ghaznavi","doi":"10.61186/iem.9.2.167","DOIUrl":"https://doi.org/10.61186/iem.9.2.167","url":null,"abstract":"Backgrounds: The primary goal of this study was to identify the potential association between COVID-19 prognosis and demographic and clinical features, underlying diseases, and drug and supplement use in patients admitted to Amir al-Momenin hospital in Zabol. Materials & Methods: This retrospective study surveyed the electronic health records of 848 COVID-19 patients hospitalized in a tertiary referral hospital in southeastern Iran from the beginning of the COVID-19 outbreak until the end of February 2021. Univariate and multiple analytical tests including unconditional and penalized logistic regressions were used for statistical analysis. Findings: Out of a total of 848 patients, 371 (43.75%) patients were female, and 477 (56.25%) patients were male. Age, underlying pulmonary and cardiovascular diseases, and loss of consciousness predicted a higher mortality rate. On the contrary, a negative chest X-ray was associated with a lower risk of death. Conclusion: Identifying predisposing factors of mortality in COVID-19 patients will help physicians provide more intensive care to those at higher risk of death by classifying patients based on risk factors and underlying diseases.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139352733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mokhtari, A. Mojtahedi, N. Mahdieh, A. Jafari, Zahra Atrkar Roushan, M. Arya
Article Type Original Article 1 Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran 2 Cardiogenetic Research Center, Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran 3 Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran 4 School of Medicine, Guilan University of Medical Sciences, Rasht, Iran 5 Anatomical and clinical pathologist, Fellowship of dermatopathology, Head of Sina Pathobiology Lab, Yazd, Iran. * Correspondence Department of Microbiology, School of Medicine, Guilan University Campus, 7th Km of Rasht-Tehran Highway, Rasht, Guilan, Iran. E-mail: mojtahedi.a@iums.ac.ir Article History Received: January 06, 2023 Accepted: February 21, 2023 Published: March 10, 2023 Backgrounds: Carbapenem resistance among Pseudomonas aeruginosa strains is alarming. This study aimed to investigate the relative frequency of carbapenem-resistant P. aeruginosa strains by phenotypic and genotypic methods. Materials & Methods: The antibiotic susceptibility pattern of 60 P. aeruginosa isolates was determined by disk diffusion method (Kirby-Bauer). BD Phoenix automated microbiology system was used to identify carbapenem-resistant isolates, and the minimum inhibitory concentration (MIC) was determined using E-Test. In addition, mCIM (modified carbapenem inactivation method) phenotypic test was performed to evaluate carbapenem resistance genes in P. aeruginosa isolates. The prevalence of metallo-beta-lactamase (MβL) genes in carbapenem-resistant P. aeruginosa isolates was determined using conventional polymerase chain reaction (PCR). Findings: The frequency of carbapenem-resistant P. aeruginosa isolates was 36% (22 of 60). The highest resistance was observed to imipenem and meropenem (36.6%), and the highest sensitivity was observed to amikacin (75%). All carbapenem-resistant P. aeruginosa isolates were confirmed by the BD Phoenix automated system (MIC>8 μg/mL for imipenem and meropenem), E-test (MIC ˂ 32 μg/mL), and mCIM assay (the growth inhibition zone diameter was 6-8 mm). In carbapenem-resistant P. aeruginosa isolates, the frequency of blaVIM, blaIMP, and blaSPM genes was 9.1% (2 of 22), 4.5% (1 of 22), and 4.5% (1 of 22), respectively. BlaKPC and blaNDM genes were not found in any of the isolates. Conclusion: Based on the present study results, all phenotypic tests used to identify carbapenemase-producing isolates had the same sensitivity (100%) and specificity (100%). Copyright@ 2023, TMU Press. This open-access article is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits Share (copy and redistribute the material in any medium or format) and Adapt (remix, transform, and build upon the material) under the Attribution-NonCommercial terms. 10.52547/iem.9.1.55
{"title":"Evaluation of the Relative Frequency of Carbapenemase Genes by Phenotypic and Genotypic Methods in Pseudomonas aeruginosa Isolates from Patients with Open Heart Surgery in Iran","authors":"M. Mokhtari, A. Mojtahedi, N. Mahdieh, A. Jafari, Zahra Atrkar Roushan, M. Arya","doi":"10.52547/iem.9.1.55","DOIUrl":"https://doi.org/10.52547/iem.9.1.55","url":null,"abstract":"Article Type Original Article 1 Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran 2 Cardiogenetic Research Center, Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran 3 Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran 4 School of Medicine, Guilan University of Medical Sciences, Rasht, Iran 5 Anatomical and clinical pathologist, Fellowship of dermatopathology, Head of Sina Pathobiology Lab, Yazd, Iran. * Correspondence Department of Microbiology, School of Medicine, Guilan University Campus, 7th Km of Rasht-Tehran Highway, Rasht, Guilan, Iran. E-mail: mojtahedi.a@iums.ac.ir Article History Received: January 06, 2023 Accepted: February 21, 2023 Published: March 10, 2023 Backgrounds: Carbapenem resistance among Pseudomonas aeruginosa strains is alarming. This study aimed to investigate the relative frequency of carbapenem-resistant P. aeruginosa strains by phenotypic and genotypic methods. Materials & Methods: The antibiotic susceptibility pattern of 60 P. aeruginosa isolates was determined by disk diffusion method (Kirby-Bauer). BD Phoenix automated microbiology system was used to identify carbapenem-resistant isolates, and the minimum inhibitory concentration (MIC) was determined using E-Test. In addition, mCIM (modified carbapenem inactivation method) phenotypic test was performed to evaluate carbapenem resistance genes in P. aeruginosa isolates. The prevalence of metallo-beta-lactamase (MβL) genes in carbapenem-resistant P. aeruginosa isolates was determined using conventional polymerase chain reaction (PCR). Findings: The frequency of carbapenem-resistant P. aeruginosa isolates was 36% (22 of 60). The highest resistance was observed to imipenem and meropenem (36.6%), and the highest sensitivity was observed to amikacin (75%). All carbapenem-resistant P. aeruginosa isolates were confirmed by the BD Phoenix automated system (MIC>8 μg/mL for imipenem and meropenem), E-test (MIC ˂ 32 μg/mL), and mCIM assay (the growth inhibition zone diameter was 6-8 mm). In carbapenem-resistant P. aeruginosa isolates, the frequency of blaVIM, blaIMP, and blaSPM genes was 9.1% (2 of 22), 4.5% (1 of 22), and 4.5% (1 of 22), respectively. BlaKPC and blaNDM genes were not found in any of the isolates. Conclusion: Based on the present study results, all phenotypic tests used to identify carbapenemase-producing isolates had the same sensitivity (100%) and specificity (100%). Copyright@ 2023, TMU Press. This open-access article is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits Share (copy and redistribute the material in any medium or format) and Adapt (remix, transform, and build upon the material) under the Attribution-NonCommercial terms. 10.52547/iem.9.1.55","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84447796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fahimeh Maleki, M. Akbari, M. Arjomandzadegan, A. Ahmadi
{"title":"Investigation of Metallobetalactamase (blaIMP & blaVIM) and Carbapenemase (blaKPC & blaGES) Genes in Gram Negative Rods Isolated from Cancer Patients","authors":"Fahimeh Maleki, M. Akbari, M. Arjomandzadegan, A. Ahmadi","doi":"10.52547/iem.9.1.43","DOIUrl":"https://doi.org/10.52547/iem.9.1.43","url":null,"abstract":"","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86158216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Akinkunmi, S. Ajoseh, C. Fakorede, R. Abegunrin, W. Salami, Aminat K. Lawal, K. Akinyemi
(n=6, 10.91%), Proteus species (n=13, 23.64%), Serratia species (n=7, 12.73%), and Citrobacter species (n=2, 3.64%). In this study, the detection rate of Salmonella isolates in positive blood culture samples using qPCR and inv A gene primer set was 100%. Salmonella isolates were %100 resistant to ceftazidime, cefotaxime, and doripenem. Multidrug resistance (MDR) was observed in Salmonella and other bacterial isolates. Conclusion: In this study, qPCR using the inv A primer set was found to be highly specific for Salmonella detection. All the bloodstream bacterial pathogens in this study were MDR; thus, there is a need for continuous evaluation of antibiotics in medical settings. Further molecular studies on these bacterial isolates is essential.
{"title":"Prevalence of Salmonella Bloodstream Infection and Antimicrobial Resistance in Lagos, Nigeria.","authors":"F. Akinkunmi, S. Ajoseh, C. Fakorede, R. Abegunrin, W. Salami, Aminat K. Lawal, K. Akinyemi","doi":"10.52547/iem.9.1.1","DOIUrl":"https://doi.org/10.52547/iem.9.1.1","url":null,"abstract":"(n=6, 10.91%), Proteus species (n=13, 23.64%), Serratia species (n=7, 12.73%), and Citrobacter species (n=2, 3.64%). In this study, the detection rate of Salmonella isolates in positive blood culture samples using qPCR and inv A gene primer set was 100%. Salmonella isolates were %100 resistant to ceftazidime, cefotaxime, and doripenem. Multidrug resistance (MDR) was observed in Salmonella and other bacterial isolates. Conclusion: In this study, qPCR using the inv A primer set was found to be highly specific for Salmonella detection. All the bloodstream bacterial pathogens in this study were MDR; thus, there is a need for continuous evaluation of antibiotics in medical settings. Further molecular studies on these bacterial isolates is essential.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86896565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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