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THE USE OF DOUBLED HAPLOID TECHNOLOGY FOR LINES DEVELOPMENT IN MAIZE BREEDING 双单倍体技术在玉米选育中的应用
Pub Date : 2022-06-20 DOI: 10.18512/rbms2022v21e1289
R. S. Trindade
In maize, doubled haploid (DH) technology is used in breeding programs to obtain lines, the primary input for developing new cultivars. This review aims to present the main points related to using this technology in maize, the steps for its application, and the advantages and implications of using DH lines in maize breeding programs.
在玉米中,双单倍体(DH)技术被用于育种计划以获得品系,这是开发新品种的主要投入。本文旨在介绍在玉米中使用该技术的要点、应用步骤,以及在玉米育种计划中使用DH系的优势和意义。
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引用次数: 3
EFFECT OF EARWORM INJURIES ON FUMONISINS PRODUCTION IN Bt AND NON-Bt MAIZE 虫害对Bt和非Bt玉米伏马菌素产量的影响
Pub Date : 2022-06-07 DOI: 10.18512/rbms2022v21e1241
S. M. Mendes, Marco Aurélio GUERRA PIMENTEL, Valéria Aparecida Vieira Queiroz, D. D. da Silva, R. V. da Costa
Tests were carried out to assess fumonisins production due to lepidopteran feeding on the ears of the hybrid DKB 390VTPRO (Cry1A105/ Cry2Ab2) and in its non-Bt isogenic version. Maize ears were harvested and classified according to injury size, and fumonisin levels for each injury class were quantified. There were no significant differences between Bt maize and non-Bt maize in fumonisin production. However, the injury size due to earworm feeding was significant for fumonisin occurrence. The greater the injury, the higher the fumonisins level.
试验评估了鳞翅目昆虫取食杂交种DKB 390VTPRO (Cry1A105/ Cry2Ab2)及其非bt等基因版本的耳朵所产生的伏马毒素。收获玉米穗,根据伤害大小进行分类,并对每个伤害类别的伏马菌素水平进行量化。Bt玉米与非Bt玉米在伏马菌素产量上无显著差异。然而,耳虫取食造成的损伤大小对伏马菌素的发生有显著影响。损伤越大,伏马菌素水平越高。
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引用次数: 0
ESTIMATES OF MEANS COMPONENTS FOR MAIZE GRAIN-FILLING TRAITS 玉米籽粒灌浆性状均值成分的估计
Pub Date : 2022-06-01 DOI: 10.18512/rbms2022v21e1229
Rita DE Kássia SIQUEIRA TEIXEIRA, Reberth Renato DA SILVA, M. Oliveira, B. L. Carvalho, Magno Antônio Patto Ramalho
One of the strategies to increase maize yield is to select lines that present a higher matter accumulation rate in the grains. This work was carried out to identify the population with the most significant potential to obtain this line type. For this, nine commercial hybrids’ F1 and F2 generations were evaluated in four environments, involving two years and two seasons each year. The traits evaluated were the number of days to female flowering (NDF) and physiological maturity (NDPM), dry matter accumulation rate (RATE), and grain yield (YLD). For each trait, the contribution of the loci in homozygosity (m+a) and heterozygosis (d) was estimated. NDF, NDPM, and RATE predominated an additive effect with a higher m+a estimate. Regarding YLD, the estimate of d was much higher than m+a, indicating the greater importance of dominance. Hybrid 5 is the most promising for obtaining the segregating population because it associates a high m+a, and d estimate that, although it was not among the highest, represented 56.03% of the average. For this hybrid, considering the YLD, the mean of the lines in F∞ will be the highest and associated with sufficient variability in the population to enable successful selection.
提高玉米产量的策略之一是选择在籽粒中物质积累率较高的品系。开展这项工作是为了确定最有可能获得这种线型的群体。为此,九个商业杂交种的F1和F2代在四个环境中进行了评估,每年涉及两年和两个季节。所评估的性状为雌性开花天数(NDF)和生理成熟度(NDPM)、干物质积累率(rate)和籽粒产量(YLD)。对于每个性状,估计基因座在纯合性(m+a)和杂合性(d)中的贡献。NDF、NDPM和RATE以m+a估计值较高的加性效应为主。关于YLD,d的估计值远高于m+a,表明优势更重要。杂交种5是获得分离种群最有希望的,因为它与高m+a和d相关,尽管它不是最高的,但估计它代表了平均值的56.03%。对于这种杂交种,考虑到YLD,F∞中的系的平均值将是最高的,并且与种群中的足够可变性相关,从而能够成功选择。
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引用次数: 0
PHENOTYPIC SELECTION OF GRAIN SORGHUM RESTORER LINES 高粱恢复系的表型选择
Pub Date : 2022-05-16 DOI: 10.18512/rbms2022v21e1230
Bruno Henrique MINGOTE JULIO, N. N. L. D. Parrella, C. V. Santos, K. J. Silva, Alexon Fernandes Campos, Iasmin Marrony Damasceno Reis, Paula Silva Mirante, Cícero Beserra de Menezes
– This study aimed to select grain sorghum R lines based on grain yield and disease traits. The experiment consisted of 360 experimental R lines and two checks cultivars CMSXS180R and 9503062R. The experimental design used was of augmented blocks. The evaluated characteristics were plant height, days to flowering, thousand-grain mass, grain yield, and evaluation of leaf blight ( Exserohilum turcicum ) and anthracnose ( Colletotrichum graminicola ). In the analysis of variance, the interaction between Lines and checks was significant for all characteristics evaluated. All traits showed heritability above 70%, except a thousand-grain mass below 60%. In this work, the lines that obtained the best means were L57 and L74, considered earlier, with plant height ideal for grain and a high grain yield and resistance to foliar diseases.
-本研究旨在根据籽粒产量和病害性状选育高粱R系。试验由360个试验R系和两个对照品种CMSXS180R和9503062R组成。实验设计采用增广块。评价指标为株高、开花天数、千粒重、产量、叶枯病(exserhilum turcicum)和炭疽病(Colletotrichum graminicola)评价。在方差分析中,线和检验之间的相互作用对所有被评估的特征都是显著的。除千粒重低于60%外,其余性状的遗传率均在70%以上。在本研究中,较早考虑的L57和L74系获得了最好的育种手段,它们株高理想,产量高,抗叶面病害能力强。
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引用次数: 0
EDITING GENOMES VIA CRISPR/CAS9 AND APPLICATIONS IN MAIZE IMPROVEMENT CRISPR/CAS9基因编辑及其在玉米改良中的应用
Pub Date : 2022-05-06 DOI: 10.18512/rbms2022v21e1284
Kamila Ellen SOUZA DE OLIVEIRA, C. Ribeiro, Andrea ALMEIDA CARNEIRO, Newton Portilho Carneiro
The CRISPR/Cas9 system is a gene editing method capable of accurately locating and altering a specific sequence of a genome. Currently, this technique stands out as a promising biotechnological tool for the improvement of different crops, overcoming the limitations of conventional breeding methods and accelerating the development of plants’ agronomic characteristics. The basic principle of genome editing via CRISPR is to cause a double-stranded DNA break at a predetermined location by a sgRNA (single guide RNA) and through internal repair mechanisms cause mutations and, consequently, genetic variability. In prokaryotic organisms, it is an immune system that naturally protects against viral infection and has been modified to create different applications in higher organisms in the field of medicine and agriculture. In agriculture, it has been used in a large number of mono and dicotyledonous plants, including those of great commercial value such as soybean, cotton and maize. In maize, this technique has been used as a biotechnological tool for increasing production, quality, nutritional value, tolerance to biotic and abiotic stresses. CRISPR-Cas9 technology has enormous potential to contribute to increased food production with great benefit to the environment. This review explores the bases for the use of CRISPR/Cas9 technology to generate genetic variability and its use in the development of maize cultivars with better agronomic characteristics.
CRISPR/Cas9系统是一种能够准确定位和改变基因组特定序列的基因编辑方法。目前,这项技术是一种很有前途的生物技术工具,可用于改良不同作物,克服传统育种方法的局限性,加速植物农艺特性的发展。通过CRISPR进行基因组编辑的基本原理是通过sgRNA(单引导RNA)在预定位置导致双链DNA断裂,并通过内部修复机制导致突变,从而导致遗传变异。在原核生物中,它是一种自然保护免受病毒感染的免疫系统,并经过改造,在医学和农业领域的高等生物中产生了不同的应用。在农业中,它已被用于大量的单子叶和双子叶植物,包括那些具有巨大商业价值的植物,如大豆、棉花和玉米。在玉米中,这项技术已被用作提高产量、质量、营养价值、对生物和非生物胁迫耐受性的生物技术工具。CRISPR-Cas9技术具有巨大的潜力,有助于提高粮食产量,对环境有很大的好处。本文探讨了利用CRISPR/Cas9技术产生遗传变异的基础及其在培育具有更好农艺性状的玉米品种中的应用。
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引用次数: 0
CONTROL EFFICACY AND PHYTOSOCIOLOGICAL CHARACTERIZATION OF WEEDS AS A FUNCTION OF POST-EMERGENCE HERBICIDES APPLIED TO MAIZE 玉米出苗后除草剂对杂草的防治效果及植物社会学特性
Pub Date : 2022-05-02 DOI: 10.18512/rbms2022v21e1240
A. F. da Silva, Vitor Abreu Padrão, G. Concenço, L. Galon, I. Aspiazú
S Studies evaluating the effectiveness of herbicides on weed control, coupled with the sustainability of the weed management system, are of great importance. The present study aims to evaluate the efficacy of post-emergence herbicides applied to maize while inferring the sustainability of the treatments employing an ecological approach. The experiment was installed in a randomized block design with four replications. The treatments consisted of the application of atrazine at the dose of 1500 g ha-1; atrazine + mesotrione at 1500 + 72 g ha-1; atrazine + tembotrione at 1500 + 100.8 g ha-1; atrazine + nicosulfuron at 1500 + 22.5 g ha-1; atrazine + glyphosate at 1500 g ha-1 + 792.5 g ha-1, in addition to weeded and infested control treatments. The herbicides were applied at the 4 leaf stage of maize. The phytotoxicity and control efficacy was assessed 7, 14, 21, and 28 days after applying treatments (DAT). In addition, a phytosociological survey of all plots was carried out in the last assessment. Herbicides did not cause phytotoxicity symptoms to the crop. However, associations of atrazine with glyphosate and atrazine with tembotrione promoted the greater effectiveness of weed control. Despite being classified as one of the treatments with better effectiveness, the association between glyphosate and atrazine, caused a more significant reduction in the diversity of the plant community, and alternative weed management practices should be applied to maize cropping fields to avoid weed selection.
S评估除草剂控制杂草的有效性以及杂草管理系统的可持续性的研究具有重要意义。本研究旨在评估玉米出苗后除草剂的效果,同时采用生态学方法推断处理的可持续性。实验采用随机分组设计,共进行四次重复。治疗包括施用剂量为1500g ha-1的阿特拉津;阿特拉津+中三酮1500+72gha-1;阿特拉津+替莫三酮,1500+100.8 g ha-1;阿特拉津+烟嘧磺隆1500+22.5 g ha-1;阿特拉津+草甘膦1500 g ha-1+792.5 g ha-1,除除草和虫害对照处理外。除草剂在玉米4叶期施用。在施用处理(DAT)后7、14、21和28天评估植物毒性和控制效果。此外,在上次评估中对所有地块进行了植物社会学调查。除草剂不会对作物造成植物毒性症状。然而,阿特拉津与草甘膦、阿特拉津和替博替翁的联合作用促进了杂草控制的更大效果。尽管草甘膦和阿特拉津被列为有效性较好的处理方法之一,但它们之间的关联导致了植物群落多样性的显著降低,因此应在玉米种植区采用替代杂草管理方法,以避免杂草选择。
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引用次数: 0
GENOMIC SELECTION AS A TOOL FOR MAIZE CULTIVARS DEVELOPMENT 基因组选择作为玉米品种发育的工具
Pub Date : 2022-04-29 DOI: 10.18512/rbms2022v21e1285
C. D. Marinho, I. Coelho, M. A. Peixoto, G. A. C. CARVALHO JUNIOR, Marcio Fernando Ribeiro Resende Júnior
The ability to predict genotypes that have not yet been tested is always a target of plant breeders. Over the last twenty years, many studies presented genomic selection (GS) as a tool contributing to this goal. Currently, many research papers have shown encouraging results in the application of GS. However, there are few examples of long-term, successful applications of GS in plant breeding programs. Furthermore, for breeders and researchers considering the application of GS, there are a series of important considerations on how to adapt a breeding program to maximize the benefit of GS, aiming to reduce the costs and maximize the genetic gains. Under this perspective, we present a review with a general view about applied GS in maize breeding, future perspectives of this technique, and an applied study case of a breeding program using GS. We attempt to provide a brief review of the literature with recent developments, as well as a discussion involving the number of markers required to deploy GS, the different statistical approaches to create GS models, the different ways to define training populations, and the incorporation of non-additive effects and genotype by environment interaction. We end with general recommendations and conclusions about some critical points about adopting GS in maize breeding.
预测尚未测试的基因型的能力一直是植物育种家的目标。在过去的二十年里,许多研究将基因组选择(GS)作为实现这一目标的工具。目前,许多研究论文在GS的应用方面取得了令人鼓舞的成果。然而,很少有GS在植物育种项目中长期成功应用的例子。此外,对于考虑GS应用的育种家和研究人员来说,如何调整育种计划以最大限度地提高GS的效益,以降低成本并最大限度地增加遗传收益,还有一系列重要的考虑因素。在这一视角下,我们对GS在玉米育种中的应用、该技术的未来前景以及使用GS的育种计划的应用研究案例进行了综述。我们试图简要回顾文献和最新进展,并讨论部署GS所需的标记数量,创建GS模型的不同统计方法,定义训练群体的不同方法,以及通过环境相互作用引入非加性效应和基因型。最后,我们对在玉米育种中采用GS的一些关键点提出了一般性的建议和结论。
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引用次数: 0
A COMMERCIAL BREEDING PERSPECTIVE OF MAIZE IMPROVEMENT FOR DROUGHT STRESS TOLERANCE 玉米抗旱性改良的商业育种展望
Pub Date : 2022-04-18 DOI: 10.18512/rbms2022v21e1277
P. Nurmberg, A. Brito, C. M. Zimmer, D. Schwantes, É. E. Mantovani, F. Bosetti, J. Rotundo, Sandra K. TRUONG, L. Borrás, Aberlardo DE LA VEGA, C. Messina, Ryan F. McCORMICK
As the most produced grain crop in the world, maize (Zea mays) isa cornerstone of the global agricultural economy. Technological innovationsin molecular genetics, environmental characterization, and predictive breedinghave continued to drive genetic gain in maize, even for target populations ofenvironments with high heterogeneity of water availability. Environments proneto drought stress remain key targets where genetic gain must continue to maintain a resilient food supply. Here we review advances towards improving maize drought tolerance; the review focused on molecular and physiological mechanisms underpinning drought tolerance, and methodologies that improve prediction of the genotype by environment interactions under drought conditions.
作为世界上产量最大的粮食作物,玉米(Zea mays)是全球农业经济的基石。分子遗传学、环境表征和预测育种方面的技术创新继续推动玉米的遗传增益,即使是对水可用性高度异质性的目标群体也是如此。易受干旱胁迫的环境仍然是关键目标,遗传增益必须继续保持有弹性的粮食供应。本文综述了提高玉米抗旱性的研究进展;本文综述了干旱耐受性的分子和生理机制,以及干旱条件下通过环境相互作用提高基因型预测的方法。
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引用次数: 3
GENETIC IMPROVEMENT IN POPCORN 爆米花的遗传改良
Pub Date : 2022-04-18 DOI: 10.18512/rbms2022v21e1272
André Luís HARTMANN CARANHATO, Rafael William ROMO TRINDADE, Renan SANTOS UHDRE, Ronald JOSÉ BARTH PINTO, Carlos Alberto Scapim, M. E. A. G. ZAGATTO PATERNIANI
Popcorn consumption in Brazil has grown significantly over the years, and genetic improvement is essential to obtain sustainable gains in multiple traits to supply this increasing demand. Thus, the objective of this review was to contribute information concerning the process of popcorn breeding in tropical regions, germplasm availability, popcorn breeding plans, the main characteristics related to popcorn quality and yield, and advances and perspectives in the process of popcorn improvement. The main focus of breeding programs is to obtain hybrids from inbred lines with high popping expansion (40 mL g-1) and yield (4.000 kg ha-1). The genetic improvement performed in Brazil has presented significant advances, mainly due to work developed in public institutions with the development of new hybrids that present more significant popping expansion and yield. However, the number of cultivars is still low, and most of them are controlled by private companies. Therefore, intrapopulation methods are recommended to develop open-pollinated varieties with high popping expansion, and this trait can be used as an early predictor of promising inbred lines to obtain superior hybrids for grain quality. Furthermore, popping expansion can be quickly recovered in backcrosses involving the cross of common maize with an inbred popcorn line.
多年来,巴西的爆米花消费量大幅增长,基因改良对于获得多种性状的可持续收益以满足日益增长的需求至关重要。因此,本综述的目的是提供有关热带地区爆米花育种过程、种质资源可用性、爆米花育种计划、与爆米花质量和产量相关的主要特征以及爆米花改良过程中的进展和前景的信息。育种计划的主要重点是从具有高爆裂扩展(40mL g-1)和产量(4.000 kg ha-1)的自交系中获得杂交种。在巴西进行的基因改良取得了重大进展,这主要归功于公共机构开发的新杂交种,这些杂交种具有更显著的爆裂扩展和产量。然而,品种数量仍然很少,而且大多数都由私营公司控制。因此,建议采用群体内方法培育具有高爆裂扩展性的开放授粉品种,该性状可作为有希望的自交系的早期预测指标,以获得优质的杂交种。此外,在普通玉米与近交系爆米花杂交的回交中,爆裂扩展可以很快恢复。
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引用次数: 1
DYNAMICS OF MILLET STRAW CULTIVATED AT DIFFERENT SOWING DATES IN THE OFF-SEASON 淡季不同播期谷子秸秆栽培动态
Pub Date : 2022-04-13 DOI: 10.18512/rbms2022vol21e1228
Marussa Cássia Favaro Boldrin, Renato Lara de Assis, K. Costa, G. Simon, Antonio Joaquim Braga PEREIRA BRAZ, Alessandro Guerra da Silva, J. Torres, Rose Luiza Moraes Tavares
The millet straw dynamics at different sowing dates in the off-season were evaluated in an experiment conducted in Rio Verde-GO. The experimental design used was randomized blocks arranged in a split-plot scheme, with four replications and four sowing dates. Five millet genotypes, two cultivars (ADR300 and ADR500) for biomass production, a dual-purpose hybrid (ADR8010) for grain and biomass production, and two hybrids (ADR9010 and ADR 9020) for grain production were used. Biomass production was performed at 0, 60, 94, 140, and 178 days after grain harvest. The millet cultivars and hybrids showed similar dry biomass production at different sowing dates, with an average production of 8,917 kg ha-1. The ADR300 and ADR500 cultivars and the ADR8010 hybrid showed similar dry biomass decomposition rates at all sowing dates. The hybrids ADR9010 and ADR9020 showed lower rates of straw decomposition in the fourth sowing date than the other genotypes. The hybrids ADR9010 and ADR9020, specific for grain production, have the potential for biomass production similar to the cultivars, regardless of the sowing dates.
在里约热内卢Verde-GO进行了谷子秸秆淡季不同播期动态变化的研究。试验设计采用分块法随机分组,4个重复,4个播期。选用5个谷子基因型,2个用于生物质生产的品种(ADR300和ADR500)、1个用于粮食和生物质生产的双用途杂交种(ADR8010)和2个用于粮食生产的杂交种(ADR9010和ADR 9020)。在收获后的第0、60、94、140和178天进行生物质生产。谷子品种和杂交种在不同播期的干生物量产量相似,平均产量为8,917 kg ha-1。ADR300、ADR500和杂种ADR8010各播期干生物量分解速率基本一致。杂种ADR9010和ADR9020在播期秸秆分解率低于其他基因型。杂种ADR9010和ADR9020具有与籽粒产量相近的产量潜力,与播期无关。
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引用次数: 0
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