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Controllable blood–brain barrier (BBB) regulation based on gigahertz acoustic streaming 基于千兆赫声流的可控血脑屏障(BBB)调节
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-12-01 DOI: 10.1063/10.0014861
Hang Qi, ShuaiHua Zhang, Jiaxue Liang, Shan He, Yanyan Wang
The blood–brain barrier (BBB) is a structural and functional barrier necessary for brain homeostasis, and it plays an important role in the realization of neural function and in protecting the brain from damage by circulating toxins and pathogens. However, the extremely dense BBB also severely limits the transport of molecules across it, which is a great hindrance to the diagnosis and treatment of central nervous system (CNS) diseases. This paper reports a new method for controllable opening of the BBB, based on the gigahertz acoustic streaming (AS) generated by a bulk acoustic wave resonant device. By adjusting the input power and working distance of the device, AS with tunable flow rate can be generated to disrupt tight junction proteins (TJs) between endothelial cells. The results obtained with this method show that the gigahertz AS promotes the penetration of dextran molecules with different molecular weights across the BBB. This work provides a new platform for studying the mechanical regulation of BBB by fluid shear forces and a new method for improving the efficiency of drug delivery.
血脑屏障(BBB)是大脑稳态所必需的结构和功能屏障,在实现神经功能和保护大脑免受循环毒素和病原体损伤方面发挥着重要作用。然而,极其密集的血脑屏障也严重限制了分子在血脑屏障中的运输,这对中枢神经系统(CNS)疾病的诊断和治疗是一个巨大的障碍。本文报道了一种基于体声波谐振装置产生的千兆赫声流(AS)的可控开启BBB的新方法。通过调节设备的输入功率和工作距离,可以产生具有可调流速的AS,以破坏内皮细胞之间的紧密连接蛋白(TJs)。用这种方法获得的结果表明,千兆赫AS促进了具有不同分子量的右旋糖酐分子穿过血脑屏障。这项工作为研究流体剪切力对血脑屏障的机械调节提供了一个新的平台,也为提高药物递送效率提供了一种新的方法。
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引用次数: 2
Multi-temperature modeling of femtosecond laser pulse on metallic nanoparticles accounting for the temperature dependences of the parameters 考虑参数温度依赖性的飞秒激光脉冲在金属纳米粒子上的多温度建模
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-12-01 DOI: 10.1063/10.0013776
V. Pustovalov
This review considers the fundamental dynamical processes of metal nanoparticles during and after the impact of a femtosecond laser pulse on a nanoparticle, including the absorption of photons. Understanding the sequence of events after photon absorption and their timescales is important for many applications of nanoparticles. Various processes are discussed, starting with optical absorption by electrons, proceeding through the relaxation of the electrons due to electron–electron scattering and electron–phonon coupling, and ending with the dissipation of the nanoparticle energy into the environment. The goal is to consider the timescales, values, and temperature dependences of the electron heat capacity and the electron–phonon coupling parameter that describe these processes and how these dependences affect the electron energy relaxation. Two- and four-temperature models for describing electron–phonon relaxation are discussed. Significant emphasis is paid to the proposed analytical approach to modeling processes during the action of a femtosecond laser pulse on a metal nanoparticle. These consider the temperature dependences of the electron heat capacity and the electron–phonon coupling factor of the metal. The entire process is divided into four stages: (1) the heating of the electron system by a pulse, (2) electron thermalization, (3) electron–phonon energy exchange and the equalization of the temperature of the electrons with the lattice, and (4) cooling of the nanoparticle. There is an appropriate analytical description of each stage. The four-temperature model can estimate the parameters of the laser and nanoparticles needed for applications of femtosecond laser pulses and nanoparticles.
这篇综述考虑了飞秒激光脉冲撞击纳米颗粒期间和之后金属纳米颗粒的基本动力学过程,包括光子的吸收。了解光子吸收后的事件序列及其时间尺度对于纳米颗粒的许多应用非常重要。讨论了各种过程,从电子的光学吸收开始,经过电子-电子散射和电子-声子耦合引起的电子弛豫,最后纳米颗粒能量耗散到环境中。目标是考虑描述这些过程的电子热容和电子-声子耦合参数的时间尺度、值和温度依赖性,以及这些依赖性如何影响电子能量弛豫。讨论了描述电子-声子弛豫的两个和四个温度模型。在飞秒激光脉冲作用于金属纳米颗粒期间,所提出的建模过程的分析方法受到了极大的重视。这些考虑了金属的电子热容和电子-声子耦合因子的温度依赖性。整个过程分为四个阶段:(1)通过脉冲加热电子系统,(2)电子热化,(3)电子-声子能量交换和电子与晶格的温度均衡,以及(4)纳米颗粒的冷却。每个阶段都有适当的分析描述。四温度模型可以估计飞秒激光脉冲和纳米颗粒应用所需的激光和纳米颗粒的参数。
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引用次数: 2
Demands and technical developments of clinical flow cytometry with emphasis in quantitative, spectral, and imaging capabilities 临床流式细胞术的需求和技术发展,重点是定量、光谱和成像能力
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-12-01 DOI: 10.1063/10.0015301
Ting Zhang, M. Gao, Xiao Chen, Chiyuan Gao, Shilun Feng, Deyong Chen, Junbo Wang, Xiaosu Zhao, Jian Chen
As the gold-standard method for single-cell analysis, flow cytometry enables high-throughput and multiple-parameter characterization of individual biological cells. This review highlights the demands for clinical flow cytometry in laboratory hematology (e.g., diagnoses of minimal residual disease and various types of leukemia), summarizes state-of-the-art clinical flow cytometers (e.g., FACSLyricTM by Becton Dickinson, DxFLEX by Beckman Coulter), then considers innovative technical improvements in flow cytometry (including quantitative, spectral, and imaging approaches) to address the limitations of clinical flow cytometry in hematology diagnosis. Finally, driven by these clinical demands, future developments in clinical flow cytometry are suggested.
作为单细胞分析的金标准方法,流式细胞术能够对单个生物细胞进行高通量和多参数表征。本综述强调了实验室血液学对临床流式细胞术的需求(例如,微小残留疾病和各种类型白血病的诊断),总结了最先进的临床流式细胞仪(例如,Becton Dickinson的faclyrictm, Beckman Coulter的DxFLEX),然后考虑了流式细胞术的创新技术改进(包括定量,光谱和成像方法),以解决临床流式细胞术在血液学诊断中的局限性。最后,在这些临床需求的驱动下,对临床流式细胞术的未来发展提出了建议。
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引用次数: 2
Conductive polymer hydrogel-coated nanopipette sensor with tunable size 尺寸可调的导电聚合物水凝胶包覆纳米吸管传感器
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-12-01 DOI: 10.1063/10.0016501
Lin Li, F. Zhou, Q. Xue
Nanopipette-based sensors are one of the most effective tools for detecting nanoparticles, bioparticles, and biomolecules. Quantitative analysis of nanoparticles with different shapes and electrical charges is achieved through measurement of the blockage currents that occur when particles pass through the nanopore. However, typical nanopipette sensors fabricated using a conventional needle-pulling method have a typical pore-diameter limitation of around 100 nm. Herein, we report a novel conductive hydrogel-composited nanopipette sensor with a tunable inner-pore diameter. This is made by electrodepositing poly(3,4-ethylenedioxythiophene) polystyrene sulfonate onto the surface of a nanopipette with a prefabricated sacrificial copper layer. Because of the presence of copper ions, the conductive polymer can stably adhere to the tip of the nanopipette to form a nanopore; when nanoparticles pass through the conductive nanopore, more distinct blocking events are observed. The size of the nanopore can be changed simply by adjusting the electrodeposition time. In this way, suitable nanopores can be obtained for highly sensitive screening of a series of particles with diameters of the order of tens of nanometers.
基于纳米管的传感器是检测纳米颗粒、生物粒子和生物分子最有效的工具之一。通过测量颗粒通过纳米孔时产生的阻塞电流,可以对具有不同形状和电荷的纳米颗粒进行定量分析。然而,使用传统的针刺方法制造的典型纳米吸管传感器具有典型的孔径限制在100nm左右。在此,我们报告了一种具有可调内孔径的新型导电水凝胶复合纳米吸管传感器。这是通过电沉积聚(3,4-乙烯二氧噻吩)聚苯乙烯磺酸盐到带有预制牺牲铜层的纳米管表面而制成的。由于铜离子的存在,导电聚合物可以稳定地附着在纳米吸管的尖端,形成纳米孔;当纳米颗粒通过导电纳米孔时,观察到更明显的阻塞事件。通过调整电沉积时间,可以简单地改变纳米孔的大小。通过这种方法,可以获得合适的纳米孔,对直径为几十纳米的一系列颗粒进行高灵敏度筛选。
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引用次数: 1
Superhydrophilic–superhydrophobic patterned surfaces: From simplified fabrication to emerging applications 超亲水-超疏水图案化表面:从简化制造到新兴应用
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-09-01 DOI: 10.1063/10.0013222
Hao Chen, Xiaoping Li, Dachao Li
Superhydrophilic–superhydrophobic patterned surfaces constitute a branch of surface chemistry involving the two extreme states of superhydrophilicity and superhydrophobicity combined on the same surface in precise patterns. Such surfaces have many advantages, including controllable wettability, enrichment ability, accessibility, and the ability to manipulate and pattern water droplets, and they offer new functionalities and possibilities for a wide variety of emerging applications, such as microarrays, biomedical assays, microfluidics, and environmental protection. This review presents the basic theory, simplified fabrication, and emerging applications of superhydrophilic–superhydrophobic patterned surfaces. First, the fundamental theories of wettability that explain the spreading of a droplet on a solid surface are described. Then, the fabrication methods for preparing superhydrophilic–superhydrophobic patterned surfaces are introduced, and the emerging applications of such surfaces that are currently being explored are highlighted. Finally, the remaining challenges of constructing such surfaces and future applications that would benefit from their use are discussed.
超亲水-超疏水图案化表面构成了表面化学的一个分支,涉及在同一表面上以精确图案组合的超亲水性和超疏水性两种极端状态。这种表面具有许多优点,包括可控的润湿性、富集能力、可及性以及操纵和图案水滴的能力,并且它们为各种新兴应用提供了新的功能和可能性,如微阵列、生物医学分析、微流体和环境保护。这篇综述介绍了超亲水-超疏水图案化表面的基本理论、简化制造和新兴应用。首先,描述了润湿性的基本理论,解释了液滴在固体表面上的扩散。然后,介绍了制备超亲水-超疏水图案化表面的制造方法,并重点介绍了目前正在探索的此类表面的新兴应用。最后,讨论了构建此类表面的剩余挑战以及从其使用中受益的未来应用。
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引用次数: 6
Design of interdigitated transducers for acoustofluidic applications 声流体应用的交叉换能器设计
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-09-01 DOI: 10.1063/10.0013405
Shuren Song, Qi Wang, Jia Zhou, A. Riaud
Interdigitated transducers (IDTs) were originally designed as delay lines for radars. Half a century later, they have found new life as actuators for microfluidic systems. By generating strong acoustic fields, they trigger nonlinear effects that enable pumping and mixing of fluids, and moving particles without contact. However, the transition from signal processing to actuators comes with a range of challenges concerning power density and spatial resolution that have spurred exciting developments in solid-state acoustics and especially in IDT design. Assuming some familiarity with acoustofluidics, this paper aims to provide a tutorial for IDT design and characterization for the purpose of acoustofluidic actuation. It is targeted at a diverse audience of researchers in various fields, including fluid mechanics, acoustics, and microelectronics.
叉指换能器(IDT)最初被设计为雷达的延迟线。半个世纪后,他们发现了作为微流体系统致动器的新生命。通过产生强声场,它们会触发非线性效应,从而能够泵送和混合流体,并在无接触的情况下移动粒子。然而,从信号处理到致动器的转变带来了一系列关于功率密度和空间分辨率的挑战,这些挑战刺激了固态声学,特别是IDT设计的令人兴奋的发展。假设对声流体学有一定的了解,本文旨在为声流体驱动的IDT设计和表征提供一个教程。它面向不同领域的研究人员,包括流体力学、声学和微电子。
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引用次数: 4
Breath monitoring, sleep disorder detection, and tracking using thin-film acoustic waves and open-source electronics 使用薄膜声波和开源电子设备进行呼吸监测、睡眠障碍检测和跟踪
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-09-01 DOI: 10.1063/10.0013471
Jethro Vernon, P. Canyelles-Pericas, H. Torun, R. Binns, Wai Pang Ng, Qiang Wu, Y. Fu
Apnoea, a major sleep disorder, affects many adults and causes several issues, such as fatigue, high blood pressure, liver conditions, increased risk of type II diabetes, and heart problems. Therefore, advanced monitoring and diagnosing tools of apnoea disorders are needed to facilitate better treatment, with advantages such as accuracy, comfort of use, cost effectiveness, and embedded computation capabilities to recognise, store, process, and transmit time series data. In this work we present an adaptation of our apnoea-Pi open-source surface acoustic wave (SAW) platform (Apnoea-Pi) to monitor and recognise apnoea in patients. The platform is based on a thin-film SAW device using bimorph ZnO and Al structures, including those fabricated as Al foils or plates, to achieve breath tracking based on humidity and temperature changes. We applied open-source electronics and provided embedded computing characteristics for signal processing, data recognition, storage, and transmission of breath signals. We show that the thin-film SAW device out-performed standard and off-the-shelf capacitive electronic sensors in terms of their response and accuracy for human breath-tracking purposes. This in combination with embedded electronics makes a suitable platform for human breath monitoring and sleep disorder recognition.
呼吸暂停是一种主要的睡眠障碍,影响到许多成年人,并导致许多问题,如疲劳、高血压、肝脏疾病、II型糖尿病风险增加和心脏病。因此,需要先进的呼吸暂停障碍监测和诊断工具,以促进更好的治疗,这些工具具有准确性、使用舒适、成本效益和嵌入式计算能力,可以识别、存储、处理和传输时间序列数据。在这项工作中,我们提出了我们的apnoea- pi开源表面声波(SAW)平台(apnoea- pi)来监测和识别患者的呼吸暂停。该平台基于薄膜SAW器件,采用双晶型ZnO和Al结构,包括那些制成Al箔或板的结构,以实现基于湿度和温度变化的呼吸跟踪。我们应用了开源电子技术,并为信号处理、数据识别、存储和传输呼吸信号提供了嵌入式计算特性。我们表明,薄膜SAW器件在人体呼吸跟踪目的的响应和准确性方面优于标准和现成的电容式电子传感器。这与嵌入式电子设备相结合,为人类呼吸监测和睡眠障碍识别提供了合适的平台。
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引用次数: 2
Piezoelectric bimorph MEMS speakers 压电双晶MEMS扬声器
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-09-01 DOI: 10.1063/10.0013406
Yiming Lang, Chengze Liu, Ahmed Fawzy, Chen Sun, Shaobo Gong, Menglun Zhang
One of the key requirements for MEMS speakers is to increase the sound pressure level (SPL) while keeping the size as small as possible. In this paper we present a MEMS speaker based on piezoelectric bimorph cantilevers that produces a higher SPL than conventional unimorph cantilever speakers. The active diaphragm size is 1.4 × 1.4 mm2. The bimorph cantilevers are connected in parallel to make full use of the actuation voltage. At 1 kHz, the measured SPL reached 73 dB and the peak SPL reached 102 dB at the resonance frequency of 10 kHz in a 711 ear simulator under a driving voltage of 10 Vrms. The total harmonic distortion of the MEMS speaker was less than 3% in the range from 100 Hz to 20 kHz. Although the absolute SPL was not the highest, this work provides a better SPL for all piezoelectric MEMS speakers.
MEMS扬声器的关键要求之一是增加声压级(SPL),同时保持尽可能小的尺寸。在本文中,我们提出了一种基于压电双晶悬臂的MEMS扬声器,该扬声器比传统的单晶悬臂扬声器产生更高的SPL。有源隔膜尺寸为1.4×1.4 mm2。双压电晶片悬臂并联连接,以充分利用驱动电压。在驱动电压为10Vrms的711耳朵模拟器中,在1kHz时,在10kHz的谐振频率下,测量的SPL达到73dB,并且峰值SPL达到102dB。MEMS扬声器的总谐波失真在100Hz到20kHz的范围内小于3%。尽管绝对SPL不是最高的,但这项工作为所有压电MEMS扬声器提供了更好的SPL。
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引用次数: 5
Acoustic radiation force on a rigid cylinder between two impedance boundaries in a viscous fluid 粘性流体中两个阻抗边界之间的刚性圆柱体上的声辐射力
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-09-01 DOI: 10.1063/10.0013562
Xinlei Liu, Zhaoyu Deng, Li Ma, Xiaozhou Liu
Acoustofluidic technology combines acoustic and microfluidic technologies to realize particle manipulation in microchannels driven by acoustic waves, and the acoustic radiation force (ARF) with boundaries is important for particle manipulation in an acoustofluidic device. In the work reported here, the ARF on a free cylinder immersed in a viscous fluid with an incident plane wave between two impedance boundaries is derived analytically and calculated numerically. The influence of multiple scattering between the particle and the impedance boundaries is described by means of image theory, the finite-series method, and the translational addition theorem, and multiple scattering is included partly in image theory. The ARF on a free rigid cylinder in a viscous fluid is analyzed by numerical calculation, with consideration given to the effects of the distances from cylinder edge to boundaries, fluid viscosity, cylinder size, and boundary reflectivity. The results show that the interaction between the two boundaries and the cylinder makes the ARF change more violently with different frequencies, while increasing the viscosity can reduce the amplitude of the ARF in boundary space. This study provides a theoretical basis for particle manipulation by the ARF in acoustofluidics.
声流体技术结合了声学和微流体技术,在声波驱动的微通道中实现粒子操纵,而具有边界的声辐射力(ARF)对于声流体设备中的粒子操纵很重要。在本文的工作中,对浸入粘性流体中的自由圆柱体的ARF进行了解析推导和数值计算,其中平面波入射在两个阻抗边界之间。利用镜像理论、有限级数法和平移加法定理描述了粒子与阻抗边界之间的多重散射的影响,多重散射部分包含在镜像理论中。通过数值计算分析了粘性流体中自由刚性圆柱体的ARF,考虑了圆柱体边缘到边界的距离、流体粘度、圆柱体尺寸和边界反射率的影响。结果表明,两个边界和圆柱体之间的相互作用使ARF在不同频率下发生更剧烈的变化,而增加粘度可以降低ARF在边界空间中的振幅。该研究为声流体学中ARF对粒子的操纵提供了理论依据。
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引用次数: 0
Ultra-precision machining of cerium-lanthanum alloy with atmosphere control in an auxiliary device 气氛控制辅助装置中铈镧合金超精密加工
IF 3.7 3区 工程技术 Q2 Engineering Pub Date : 2022-09-01 DOI: 10.1063/10.0013777
Chenyu Zhao, Sheng-Chang Wu, M. Lai
Cerium–lanthanum alloys are the main component of nickel–metal hydride batteries, and they are thus an important material in the green-energy industry. However, these alloys have very strong chemical activity, and their surfaces are easily oxidized, leading to great difficulties in their application. To improve the corrosion resistance of cerium–lanthanum alloys, it is necessary to obtain a nanoscale surface with low roughness. However, these alloys can easily succumb to spontaneous combustion during machining. Currently, to inhibit the occurrence of fire, machining of this alloy in ambient air needs to be conducted at very low cutting speeds while spraying the workpiece with a large amount of cutting fluid. However, this is inefficient, and only a very limited range of parameters can be optimized at low cutting speeds; this restricts the optimization of other cutting parameters. To achieve ultraprecision machining of cerium–lanthanum alloys, in this work, an auxiliary machining device was developed, and its effectiveness was verified. The results show that the developed device can improve the cutting speed and obtain a machined surface with low roughness. The device can also improve the machining efficiency and completely prevent the occurrence of spontaneous combustion. It was found that the formation of a build-up of swarf on the cutting tool is eliminated with high-speed cutting, and the surface roughness (Sa) can reach 5.64 nm within the selected parameters. Finally, the oxidation processes of the cerium–lanthanum alloy and its swarf were studied, and the process of the generation of oxidative products in the swarf was elucidated. The results revealed that most of the intermediate oxidative products in the swarf were Ce3+, there were major oxygen vacancies in the swarf, and the final oxidative product was Ce4+.
铈镧合金是镍氢电池的主要成分,是绿色能源产业的重要材料。然而,这些合金具有很强的化学活性,表面容易氧化,给其应用带来了很大的困难。为了提高铈镧合金的耐蚀性,必须获得低粗糙度的纳米级表面。然而,这些合金在加工过程中很容易自燃。目前,为了抑制火灾的发生,在环境空气中加工该合金需要以极低的切削速度进行,同时向工件喷射大量切削液。然而,这是低效的,只有非常有限的参数范围可以在低切削速度下进行优化;这限制了其他切削参数的优化。为了实现铈镧合金的超精密加工,研制了一种辅助加工装置,并对其有效性进行了验证。结果表明,该装置可提高切削速度,获得低粗糙度的加工表面。该装置还可以提高加工效率,完全防止自燃的发生。结果表明,高速切削消除了刀具上结块的形成,在所选参数范围内,表面粗糙度(Sa)可达5.64 nm。最后,对铈镧合金及其结块的氧化过程进行了研究,阐明了结块中氧化产物的生成过程。结果表明:金属屑中间氧化产物以Ce3+为主,金属屑中存在较大的氧空位,最终氧化产物为Ce4+。
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引用次数: 2
期刊
Nami Jishu yu Jingmi Gongcheng/Nanotechnology and Precision Engineering
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