Elucidating organic matter sources in lakes during the frozen period (FP) is crucial for understanding carbon and nitrogen cycling in global cold-region lakes. However, limited information exists on the sources and differences between suspended particulate organic matter and sediment organic matter in lakes during the FP. This study examined the stable carbon (δ13C) and nitrogen (δ15N) isotopic compositions of organic matter in suspended particulates and sediments from Daihai Lake to investigate the sources and implications of organic matter during the FP (January). Organic carbon in suspended particulates and total nitrogen in suspended particulates ranged from 0.45 to 1.22 mg/L and 0.08 to 0.20 mg/L, respectively, while organic carbon in sediments and total nitrogen in sediments ranged from 3.57 to 14.90 g/kg and 0.44 to 1.68 g/kg, respectively. Based on organic index (OI) and organic nitrogen (ON), suspended particulates (OI: 0.10 mg/L; ON: 0.12 mg/L) were slightly contaminated, whereas sediments (OI: 13.13 g/kg; ON: 1.08 g/kg) were heavily contaminated. The suspended particulate organic matter exhibited mixed source signatures, with exogenous inputs (sewage: 27.9% and soil: 22.2%) and endogenous production (phytoplankton: 25.8%). In contrast, sediment organic matter was predominantly exogenous inputs (soil: 40.3% and sewage: 26.6%). This discrepancy highlights a key process in organic matter transport and transformation under ice-covered conditions: phytoplankton-derived organic matter underwent preferential degradation during sedimentation, whereas terrestrial organic matter was more readily deposited and preserved. Notably, significant nitrogen isotope fractionation during sedimentation indicates that preferential mineralization of organic nitrogen and denitrification played key regulatory roles in the nitrogen cycling. The findings highlight the need to prioritize controlling exogenous organic matter inputs to lakes to ensure sustainable ecosystem.
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