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Sex determination from foramen magnum parameters in Thai cadaveric donor 根据泰国尸体供体的枕骨大孔参数确定性别
Q3 Medicine Pub Date : 2024-05-11 DOI: 10.1016/j.fsir.2024.100371
Natipong Chatthai , Napakorn Sangchay , Parkpoom Piyaman , Parichart Pattarapanitchai , Sirinart Chomean , Chollanot Kaset

The foramen magnum (FM) occupies a well-protected position, making it particularly intriguing for forensic research. The necessity for methods to estimate sex from cranial fragments becomes evident when only a partial skull is available for identification. The objective of this study was to assess sex differences in anthropometric measurements, including the length of the foramen magnum (FML), width of the foramen magnum (FMW), foramen magnum area (FMA), and circumference, with the aim of developing a binary logistic regression model in Thai cadaveric donors. Sixty adult dry skulls underwent both intraclass and interclass correlation analyses. Subsequently, 240 adult dry skulls of known sex (120 males and 120 females) were meticulously measured and analyzed to determine the optimal threshold for sex determination. Morphometric analysis of the FM was carried out using vernier calipers, and the area of the FM was precisely calculated. The FML, FMW, FMA, and circumference were all found to be significantly larger in males than in females. Furthermore, FM circumference exhibited an AUC (Area Under the Curve) value of 0.908, signifying it as an excellent parameter for sex discrimination. Lastly, binary logistic regression was executed. Significant sex differences in FM circumference were consistently observed, demonstrating an accuracy of 81.62% when validated in 120 dry skulls.

枕骨大孔(FM)的位置受到很好的保护,因此对法医研究特别有吸引力。当只有部分头骨可供鉴定时,根据头骨碎片估计性别的方法的必要性就显而易见了。本研究的目的是评估人体测量数据(包括枕骨大孔长度(FML)、枕骨大孔宽度(FMW)、枕骨大孔面积(FMA)和周长)的性别差异,以便在泰国尸体捐献者中建立二元逻辑回归模型。对 60 个成人干头骨进行了类内和类间相关性分析。随后,对已知性别的 240 个成人干头骨(120 个男性和 120 个女性)进行了细致的测量和分析,以确定确定性别的最佳阈值。使用游标卡尺对FM进行形态分析,精确计算FM的面积。结果发现,男性的 FML、FMW、FMA 和周长均明显大于女性。此外,FM 周长的 AUC(曲线下面积)值为 0.908,表明它是一个很好的性别鉴别参数。最后,进行了二元逻辑回归。在 120 个干燥头骨中验证后,发现 FM 周长始终存在显著的性别差异,准确率为 81.62%。
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引用次数: 0
Assessing the reliability and accuracy of sex estimation models utilizing sternal morphometry derived from computed tomography in the Ghanaian population 利用计算机断层扫描得出的胸骨形态测量结果,评估性别估计模型在加纳人口中的可靠性和准确性
Q3 Medicine Pub Date : 2024-05-01 DOI: 10.1016/j.fsir.2024.100368
Moses Banyeh , Abdul-Rafik Abdulai , Ernest Kofi Annan , Emmanuel Kofi Mensah , Charles Nkansah , Jeffrey Adom Nathan , Margaret Birago Twum , Paul Aghana Achumboro

Sex estimation models are specific to populations and cannot be generalized due to genetic and environmental variabilities. This retrospective cross-sectional study, conducted between January and September 2023 at the Tamale Teaching Hospital, included 119 (50.9 %) females and 115 (49.1 %) males aged 23–82 years. Measurements, including manubrium length (M), manubrium width (MW), sternal body length (B), combined manubrium and sternal body lengths (CL), corpus sterni width at first sternebrae (CSWS1), and corpus sterni width at third sternebrae (CSWS3), were obtained from Computerized Tomographic (CT) images of the sternum using DICOM Viewer, accurate to 0.1 cm. Subsequently, sternal area (SA) and sternal index (SI) were calculated. Univariable and stepwise multivariable discriminant function analysis (DFA) and logistic regression (LR) models were developed using a training sample (70 %), and cross-validation was performed on a holdout sample (30 %). Results showed that the linear measurements, excluding M, and sternal area were higher in males than females (P<0.001), while the sternal index was <50 % in males but >50 % in females (P<0.001). Univariable sex estimation accuracies, in cross-validation, ranged from 43.7 % to 92.9 % in DFA and 50.0–92.9 % in LR. For multivariable models, the accuracy ranges were 92.9–94.3 % in DFA and 91.6–93.0 % in LR. The sternal body length was the most accurate at 90.1 % in DFA and 90.2 % in LR, with lower sex bias (male-female) in LR than in DFA (-0.1 vs. 8.7). The sternum proves valuable for sex estimation, with sternal body length as the most accurate linear measurement. However, multivariable models, particularly LR, demonstrate higher accuracy compared to DFA.

性别估计模型是针对特定人群的,由于遗传和环境的变异性而无法推广。这项回顾性横断面研究于 2023 年 1 月至 9 月在塔马利教学医院进行,包括 119 名(50.9%)女性和 115 名(49.1%)男性,年龄在 23-82 岁之间。测量数据包括胸骨长度(M)、胸骨宽度(MW)、胸骨体长度(B)、胸骨和胸骨联合长度(CL)、第一节胸骨处胸骨宽度(CSWS1)和第三节胸骨处胸骨宽度(CSWS3),测量数据来自使用 DICOM Viewer 的胸骨计算机断层扫描(CT)图像,精确到 0.1 厘米。随后计算胸骨面积(SA)和胸骨指数(SI)。利用训练样本(70%)建立了单变量和逐步多变量判别函数分析(DFA)和逻辑回归(LR)模型,并对保留样本(30%)进行了交叉验证。结果显示,男性的线性测量值(不包括 M)和胸骨面积均高于女性(P<0.001),而胸骨指数男性为 50%,女性为 50%(P<0.001)。在交叉验证中,DFA 的单变量性别估计准确率为 43.7% 至 92.9%,LR 为 50.0% 至 92.9%。对于多变量模型,DFA 的准确率范围为 92.9%-94.3%,LR 为 91.6%-93.0%。胸骨体长的准确率最高,DFA 为 90.1%,LR 为 90.2%,LR 的性别偏差(男性-女性)低于 DFA(-0.1 vs. 8.7)。胸骨对性别估计很有价值,胸骨体长是最准确的线性测量值。然而,与 DFA 相比,多变量模型,尤其是 LR,显示出更高的准确性。
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引用次数: 0
Height and sex estimation using percutaneous humeral length among Ghanaians 利用经皮肱骨长度估算加纳人的身高和性别
Q3 Medicine Pub Date : 2024-04-26 DOI: 10.1016/j.fsir.2024.100367
Joshua Tetteh , Thomas Diby , Chrissie Stansie Abaidoo , Joseph Yorke , Juliet Robertson , James Nketsiah

Introduction

Humerus is a long bone that contributes significantly to the total height of an individual. It is sexually dimorphic and is a well-preserved bone postmortem. Height and sex are essential in establishing one's identity and can be estimated from long bones when standing height cannot be easily obtained. Previous studies report that sex and height are population-specific. Hence, the present study sought to establish the relationship between percutaneous humeral lengths and height and formulate models for height and sex determination for the Ghanaian population.

Materials and methods

Measurement of standing height and percutaneous humeral lengths were taken from 286 Ghanaian consented participants with no noticeable lower and upper extremities traumas or pathologies. The data was analysed using Microsoft Excel 2013 and SPSS version 23.0 edition. Correlation analysis was done to determine the correlation of the percutaneous humeral length with height, linear regression and binary logistic analyses were used to derive equations for predicting height and sex from percutaneous humeral length. An independent samples t-test was used to determine the means between males and females, left and right percutaneous humeral lengths.

Results

There was a strong positive correlation between standing height and left (r = 0.774) and right (r=0.759) humeral lengths. The left humeral length regression equation was a good height estimator in males (75.4%) and females (71.%). The left and right humeral lengths best predicted the sex for the population, with an accuracy of 78.0% and 77.8% for males and 53.8% and 54.6% for females, respectively.

Conclusion

This study has added to the limited data on the use of percutaneous humeral length as a substitute for the determination of standing height and sex in the Ghanaian population, and it is also a significant identification tool which can be employed in the field of forensic anthropometry.

导言umerus 是一种长骨,对个体的总身高有重大贡献。它具有性别二态性,是一种保存完好的死后骨骼。身高和性别对于确定一个人的身份至关重要,在无法轻易获得站立身高的情况下,可以通过长骨来估算。以往的研究报告表明,性别和身高具有人群特异性。因此,本研究试图确定肱骨经皮长度与身高之间的关系,并为加纳人口的身高和性别确定制定模型。材料和方法测量站立身高和肱骨经皮长度的对象是286名经同意的加纳人,他们没有明显的上下肢创伤或病变。数据使用 Microsoft Excel 2013 和 SPSS 23.0 版进行分析。相关性分析用于确定经皮肱骨长度与身高的相关性,线性回归和二元逻辑分析用于根据经皮肱骨长度得出预测身高和性别的方程。结果站立身高与左侧(r=0.774)和右侧(r=0.759)肱骨长度之间存在很强的正相关。左肱骨长度回归方程对男性(75.4%)和女性(71.%)的身高估计效果良好。结论:这项研究补充了关于使用经皮肱骨长度来替代测定加纳人口站立身高和性别的有限数据,它也是一种重要的鉴定工具,可用于法医人体测量领域。
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引用次数: 0
Survivability of IED components, suitcases, their contents and luggage containers in suitcase bombs 手提箱炸弹中简易爆炸装置部件、手提箱、箱内物品和行李箱的生存能力
Q3 Medicine Pub Date : 2024-04-15 DOI: 10.1016/j.fsir.2024.100366
Erwin Vermeij

One of the most striking attacks on a commercial passenger airliner was the bombing of Pan Am Flight 103 (PA103) on 21 December 1988. Shortly after departure, the Boeing 747 “Clipper Maid of the Seas” broke apart over the Scottish town of Lockerbie. All 259 passengers and crew were killed, along with 11 residents of Lockerbie. The bombing of flight PA103 is still subject to controversy. The origin of a crucial piece of evidence, a fragment of circuit board, is highly questioned. One of the reasons for this ongoing controversy is probably that circumstantial evidence, that led to the conviction of a Libyan, is not supported by other evidence. Almost nothing is published in the forensic literature regarding the survivability of components that make up an improvised explosive device (IED). To gain insight into the survivability of IED components, suitcases, their contents, and luggage containers, a series of controlled experiments were carried out in which suitcase bombs, positioned in different configurations in aluminium LD3 luggage containers, were detonated. From the results, it could be seen that the major part of the suitcases, their contents and the LD3 containers survived the explosion. Also, almost all components of the IED itself survived the explosion to a greater or lesser extent.

1988 年 12 月 21 日泛美航空公司 103 号航班(PA103)爆炸事件是对商业客机最引人注目的袭击之一。起飞后不久,这架波音 747 "Clipper Maid of the Seas "客机在苏格兰小镇洛克比上空解体。259 名乘客和机组人员全部遇难,还有 11 名洛克比居民。PA103 号航班被炸至今仍存在争议。一个关键证据--一块电路板碎片--的来源备受质疑。争议持续不断的原因之一可能是,导致一名利比亚人被定罪的间接证据没有得到其他证据的支持。法医文献中几乎没有关于简易爆炸装置(IED)部件存活性的内容。为了深入了解简易爆炸装置组件、手提箱、箱内物品和行李箱的存活能力,我们进行了一系列受控实验,将手提箱炸弹以不同配置放置在 LD3 铝制行李箱中引爆。实验结果表明,手提箱、箱内物品和 LD3 行李箱的大部分在爆炸中幸存下来。此外,简易爆炸装置本身的几乎所有部件都或多或少地在爆炸中幸存下来。
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引用次数: 0
Utilizing differential extraction thresholds to deduce the existence of spermatozoa in forensic casework samples 利用差异提取阈值推断法医案件样本中精子的存在
Q3 Medicine Pub Date : 2024-03-30 DOI: 10.1016/j.fsir.2024.100365
Evelyn Ridgley, Casey Dejournett, Karen Olson

Testing of evidence in an alleged sexual assault case not only seeks to address the question of who was involved, but also looks to answer the question of what biological source provided the DNA. It can be difficult to obtain positive serological data on challenging samples such as laundered items, low-level DNA samples, or sexual assault kit swabs obtained after a prolonged interval from the time of assault. In the absence of confirmatory serological results, an expert witness often cannot speak to the biological source of the DNA. In order to determine quantitation thresholds which could be used to deduce the presence of spermatozoa (sperm) within a sample, we evaluated the fractionation of male DNA utilizing our laboratory’s differential extraction method. Study samples included serial dilutions of semen and semen/saliva mixtures, post-coital and laundered samples as well as casework data from 1,729 samples that were processed using a differential extraction. Based on this data, it was determined that a sample which had at least 200 picograms of male DNA and at least 10% of the total male DNA in fraction 2 (F2, also known as the sperm-enriched or sperm fraction) could be reported as positive for the presence of sperm. No false positive results were obtained from the study-generated samples when using these thresholds to infer the presence of sperm. Additionally, samples that contained sperm, but were negative using traditional serological methods, could be detected. However, not all sperm-containing samples fractionated above both thresholds; therefore, serological testing may still be necessary to minimize false negative results. The thresholds developed here, proved reliable to deduce the presence of sperm in real casework samples.

对指控性侵犯案件中的证据进行检测,不仅是为了解决谁参与了性侵犯的问题,也是为了回答是什么生物来源提供了 DNA 的问题。对于具有挑战性的样本,例如洗过的物品、低水平的 DNA 样本或在与性侵犯发生时间相隔较长时间后获得的性侵犯工具箱拭子,可能很难获得阳性血清学数据。在没有确证血清学结果的情况下,专家证人往往无法说明 DNA 的生物来源。为了确定可用于推断样本中精子(精液)存在的定量阈值,我们利用实验室的差分提取法对男性 DNA 的分馏进行了评估。研究样本包括精液和精液/唾液混合物的系列稀释液、性交后样本和洗涤样本,以及使用差分提取法处理的 1,729 份样本的个案工作数据。根据这些数据确定,如果样本中至少含有 200 皮克的男性 DNA,且第 2 部分(F2,又称精子富集部分或精子部分)中的男性 DNA 至少占总 DNA 的 10%,则可报告样本中精子的存在呈阳性。使用这些阈值来推断精子是否存在时,研究产生的样本没有出现假阳性结果。此外,含有精子但使用传统血清学方法检测为阴性的样本也能被检测出来。不过,并非所有含精子样本的分馏结果都高于这两个阈值;因此,仍有必要进行血清学检测,以尽量减少假阴性结果。事实证明,在实际案例样本中推断精子存在的阈值是可靠的。
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引用次数: 0
Fatal myocardial infarction following pulmonary vein isolation due to atrioesophageal fistula: Case report 因无动脉食管瘘导致肺静脉隔离术后发生致命心肌梗死:病例报告
Q3 Medicine Pub Date : 2024-03-27 DOI: 10.1016/j.fsir.2024.100364
Conny Hartmann , Joelle Tschui , Kristina Bauer , Jeremias Klaus , Wolf-Dieter Zech , Christian Jackowski , Nicolas Lange-Herr

This case report describes the death of a 73-year-old man who suffered a fatal myocardial infarction following pulmonary vein isolation (PVI) for the treatment of atrial fibrillation. Post-mortem examination and imaging revealed the presence of an atrioesophageal fistula (AEF) leading to gas embolism at various sites of the heart including the right coronary artery. Atrioesophageal fistulas are rare complications of pulmonary vein isolation with high mortality rates.

本病例报告描述了一名 73 岁男性在接受肺静脉隔离术(PVI)治疗心房颤动后发生致命心肌梗死的死亡病例。死后检查和影像学检查发现存在一个寰食管瘘(AEF),导致包括右冠状动脉在内的心脏多个部位发生气体栓塞。心房食管瘘是肺静脉隔离术的罕见并发症,死亡率很高。
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引用次数: 0
Solving cold cases: The importance of new identification techniques in old cases of forensic anthropology 解决悬案:新鉴定技术在法医人类学旧案中的重要性
Q3 Medicine Pub Date : 2024-03-18 DOI: 10.1016/j.fsir.2024.100363
Gabriela Araujo dos Santos , Talita Lima de Castro Espicalsky , Leticia Regina Alfaia Custódio , Melina Calmon Silva , Eugenia Cunha

Scientific and technological developments experienced by forensic sciences have increased the possibility of human identification. Forensic anthropology has developed worldwide and has made significant progress in Brazil in the last 10 years. This context has allowed forensic medicine institutes to use new techniques to resolve cold cases of human identification. This work aimed to report the methodology, development, and partial results of the LAFOL Identifies Operation, carried out at the Forensic Anthropology and Forensic Dentistry Laboratory (LAFOL) of the Medico-Legal Institute of Porto Velho. We carried out a multidisciplinary expert approach on human skeletal remains archived over the last 20 years to identify these individuals using current scientific methods. Eighty-one cold cases of skeletonized and/or mummified human skeletal remains were reanalyzed; they were archived in the LAFOL Laboratory collection as unidentified. Anthropological analyses showed that the collection is predominantly composed of adult individuals (86.4%), between 21 and 39 years old (55.6%), and males (62.9%). In the human skeletal remains identified, forensic anthropology played a key role in the case screening process. We expected that the results presented in this work will inspire other Medico-Legal Institutes in Brazil and worldwide to establish task forces for the reanalysis of cold cases in their institutes, promoting new forensic dentistry and anthropological comparisons and collecting biological samples to feed the database of genetic profiles of missing persons.

法医学所经历的科技发展提高了人类身份鉴定的可能性。法医人类学在世界范围内得到了发展,并在过去 10 年中在巴西取得了重大进展。在此背景下,法医机构得以使用新技术来解决冷门的人类身份鉴定案件。这项工作旨在报告波尔图韦略医学法律研究所法医人类学和法医牙科实验室(LAFOL)开展的 "LAFOL 鉴定行动 "的方法、发展和部分成果。我们采用多学科专家方法对过去 20 年中归档的人类骸骨进行了鉴定,以使用当前的科学方法识别这些人的身份。我们对 81 具冷藏的骸骨和/或木乃伊化的人类骸骨进行了重新分析;这些骸骨在 LAFOL 实验室的藏品中被归档为未经鉴定的骸骨。人类学分析表明,这些遗骸主要是成年人(86.4%),年龄在 21 至 39 岁之间(55.6%),男性(62.9%)。在已确认的人类骸骨中,法医人类学在案件筛选过程中发挥了关键作用。我们希望这项工作所取得的成果能够激励巴西和世界各地的其他医学法律研究所成立工作组,重新分析其研究所中的悬案,促进新的法医牙科学和人类学比较,并收集生物样本,为失踪人员基因图谱数据库提供资料。
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引用次数: 0
Death by scythe: Where forensic pathology meets arts and symbolism 镰刀下的死亡:法医病理学与艺术和象征主义的结合
Q3 Medicine Pub Date : 2024-03-06 DOI: 10.1016/j.fsir.2024.100362
Martin Janík , Ľubomír Straka , Lenka Zátopková , Petr Hejna

We report a rare case of fatal exsanguination by a scythe. A 71-year-old man slipped while jumping over a stream and impaled himself on a 70 cm long curved steel blade of the scythe. The external examination of the body disclosed a stab wound in the left upper part of the trunk. Internally, impaling injuries involving the left thoracic walls, left lung parenchyma, and pulmonary vasculature were disclosed. Toxicology results indicated preceding alcohol use. This paper also traces and consolidates symbolic, artistic, and historical connotations of the scythe and highlights the intersection of art and science that defines the field of forensic pathology. Although exploring the relationship between the arts and pathology is not novel, intriguing links between the arts and forensic practice can broadly enhance observation and analytical skills, and help forensic professionals become more thoughtful and contextual.

我们报告了一例罕见的镰刀致命性失血病例。一名 71 岁的老人在跳过一条小溪时不慎滑倒,被镰刀上 70 厘米长的弧形钢刃刺穿。尸体外部检查发现,躯干左上部有一处刺伤。内部检查显示,左胸壁、左肺实质和肺血管有刺伤。毒理学结果表明死者曾饮酒。本文还追溯并整合了镰刀的象征意义、艺术和历史内涵,并强调了法医病理学领域所定义的艺术与科学的交叉。虽然探索艺术与病理学之间的关系并不新颖,但艺术与法医实践之间耐人寻味的联系可以广泛地提高观察和分析能力,并帮助法医专业人员变得更加深思熟虑、更有背景。
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引用次数: 0
Falls from high-altitude with no fatal injuries: Report of two autopsy cases 高空坠落未造成致命伤:两例尸检报告
Q3 Medicine Pub Date : 2024-03-05 DOI: 10.1016/j.fsir.2024.100361
Tomoka Yamaguchi, Shiori Hattori, Keisuke Mizuo, Satoshi Watanabe

We report two cases of falls from a very high altitude with no fatal injuries. Two men were found dead directly under a high bridge. Surveillance video footage indicated that they had fell approximately 120 m from the bridge to the ground. On the fall site, there was snow on the ground, and the men were completely buried in the snow. An autopsy performed at 2 days after their bodies were found, showed a small subdural hematoma in one patient and a minor pulmonary laceration in the other. However, no fatal injuries were observed. Their deaths were caused by hypothermia based directly on the difference in the coloration of the left and right cardiac blood, the bright red to reddish brown lungs, and the Wischnewski spots. The reason why no fatal injuries occurred despite falling from height was considered to be the fall onto the snow.

我们报告了两起高空坠落事故,均未造成人员死亡。两名男子被发现死于一座高桥下。监控录像显示,他们从桥上坠落到地面约 120 米。坠落地点的地面上有积雪,两人完全被埋在雪中。尸体被发现两天后进行的尸检显示,一名患者有轻微硬膜下血肿,另一名患者有轻微肺裂伤。但是,没有发现致命伤。根据左右心血颜色的差异、肺部的鲜红至红褐色以及维斯切夫斯基斑点,可以直接判定他们的死因是体温过低。高空坠落却未造成致命伤的原因被认为是坠落在雪地上。
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引用次数: 0
Concrete evidence: Analysis of aggregate and cement in a homicide investigation 具体证据:凶杀案调查中的骨料和水泥分析
Q3 Medicine Pub Date : 2024-03-02 DOI: 10.1016/j.fsir.2024.100360
Alastair Ruffell, Jennifer M. McKinley

The unusual body deposition site described comprised three elements of concealment: i) a covert stream-based ravine some 60 m from the suspect’s home; ii) partial grave dug into the ravine bank and iii) final concealment using concrete slabs. Disaggregation and sieving of concrete samples from the site, suspect residence(s) and control samples was carried out. These allowed informative exclusion of all but one control sample and provided a range of possible comparisons that may reflect the sequence of concrete slab selection, transport and use in covering the victim. The textures/colours of disaggregated, dried sediment size fractions also proved useful in conveying principles of exclusion to the court and jury at a subsequent murder trial. This work flows from basic (visual) observation of dry, cut blocks, through regular laboratory procedures of thin section work to disaggregation and size separation of aggregate-cement fractions. Graphical presentation of each analysis provided effective communication of geological science during the trial at court, concluding with a verdict of guilty by aggravated murder.

所述不寻常的尸体存放地点包括三个隐蔽要素:i) 距嫌疑人住所约 60 米的隐蔽溪谷;ii) 在溪谷岸边挖掘的部分坟墓;iii) 使用混凝土板进行的最终隐蔽。对来自现场、疑犯住所和对照样本的混凝土样本进行了分类和筛分。除一个对照样本外,这些样本都被排除在外,并提供了一系列可能的比较,这些比较可能反映了混凝土板的选择、运输和用于掩盖受害者的顺序。事实证明,在随后的谋杀案审判中,干燥沉积物粒度碎片的质地/颜色也有助于向法庭和陪审团传达排除原则。这项工作从基本的(目测)干切块观察,到薄片工作的常规实验室程序,再到骨料-水泥组分的分解和粒度分离。在法庭审判期间,每项分析的图表演示都提供了有效的地质科学交流,最后以严重谋杀罪的有罪判决结束。
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引用次数: 0
期刊
Forensic Science International: Reports
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