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Results of the 2023 rapid DNA multi-laboratory study – RapidINTEL Plus sample cartridge
Q3 Medicine Pub Date : 2024-12-01 DOI: 10.1016/j.fsir.2024.100396
Erica L. Romsos , Lilliana I. Moreno , Douglas R. Hares
Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages.
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引用次数: 0
Results of the 2023 rapid DNA multi-laboratory study – I-Chip
Q3 Medicine Pub Date : 2024-12-01 DOI: 10.1016/j.fsir.2024.100395
Erica L. Romsos , Lilliana I. Moreno , Douglas R. Hares
Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages.
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引用次数: 0
Effect of leather tanning process on stable isotopes and radiocarbon in tissues of Persian leopard: Preliminary results
Q3 Medicine Pub Date : 2024-12-01 DOI: 10.1016/j.fsir.2024.100398
Kateřina Pachnerová Brabcová , Nikola Pravdíková , Kateřina Čápová , Jaroslava Frouzová , Kristýna Hebenstreitová , Kateřina Jandová , Jaroslav Kukla , Eliška Rajmonová , Ondřej Salaba , Ivo Světlík , Dominik Šmok , Jakub Trubač , Jitka Kufnerová
The international trade in animals and their parts is a multi-billion-dollar industry. Legal trade in wild animals and their derivatives is based on sustainable practices, ensuring that specimens are taken in quantities that do not threaten population stability. Unregulated or illegal international trade can jeopardise the survival of certain species by depleting wild populations. Forensic analysis is crucial in distinguishing between legal and illegal trade, particularly by determining the geographical origin or age of a specimen through methods such as stable isotope and radiocarbon analysis. The tanning process, which converts mammalian raw hides into valuable fur skins, involves the use of aggressive chemical reagents. These reagents, if absorbed by the hair or skin, can alter the original isotopic signatures, complicating forensic efforts. The extent of these alterations is not yet fully understood. The novelty of our approach lies in the fact that, for the first time, we have measured the step-by-step changes in isotopic composition throughout each individual phase of the tanning process. Previous studies have typically focused only on raw hides and final products. Additionally, we compared different tanning methods applied to a single hide. Using a leopard skin, we documented potential isotopic shifts in 14C, 2H, 18O, 15N and 13C during the tanning process, employing accelerated mass spectrometry and stable isotope mass spectrometry. Our preliminary findings suggest that the tanning process induces slight shifts in both stable and radioactive isotope ratios.
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引用次数: 0
Forensic archaeology and illegal familial repatriation and burial of a fallen WW1 soldier, Nièvre, central-east France
Q3 Medicine Pub Date : 2024-12-01 DOI: 10.1016/j.fsir.2024.100397
Marine MEUCCI DULY , Caroline Costedoat , Julien Fargettas , Emeline Verna
The context of past conflicts, especially World War I or World War II, is treated differently in various countries. In France, the discovery of military human remains falls under the responsibility of the Ministry of the Armed Forces. Collaborations between the Ministry and national Universities allow for interdisciplinary studies that meet international scientific standards. This case report highlights the importance of interdisciplinary approaches to achieve the identification of skeletonized individuals. An abandoned grave vault in a small town of Nièvre, central-east France, uncovered three skeletons associated with militaria. Initially, the remains were believed to belong to three Senegalese Riflemen executed nearby in 1940, but some of the evidence appeared contradictory. The aim of this study is to determine whether these three skeletons were the three Senegalese Riflemen or other individuals. Through an interdisciplinary approach, combining the position of the bodies in the vault, anthropological analysis of the human remains, and archival research, it was determined that the three bodies belonged to the same family. The remains were those of an illegally repatriated body of a soldier from the battlefields of WW1, together with his parents. It is suggested that they illegally repatriated him from the battlefield/cemetery and then buried him in the family vault, and later, when the parents passed away, they were also buried alongside him. This case illustrates the profound violence of the First World War and the many family losses.

Simple summary

The identities of three skeletons discovered buried together in a graveyard were investigated due to the presence of militaria artefacts associated with one of them, revealing a rare case of illegal World War I fallen soldier familial repatriation.
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引用次数: 0
Survey of bloodstain pattern analysts regarding pattern classifications on assorted fabrics 关于各种织物上血迹图案分类的血迹图案分析师调查
Q3 Medicine Pub Date : 2024-11-20 DOI: 10.1016/j.fsir.2024.100394
Dylan J. Drazdik , David M. Hammond , Travis J. Worst , Crystal M. Oechsle
Because bloodstain pattern analysis (BPA) on absorbent surfaces, such as fabrics, is far more complex than its application on hard, nonporous surfaces, pattern type may be interpreted differently between fabric substrates and by analysts with more or less experience. Four bloodstain pattern types (impact spatter, transfer, drip stains, and cast-off) were created on seven fabrics (cotton, satin polyester, rayon, blended polyester/spandex, blended nylon/spandex, blended modal/polyester/spandex, and terry cloth), foamboard, or butcher paper and then photographed. A survey questionnaire that asked respondents to classify 16 patterns as to general pattern type, specific pattern type, and directionality was developed and disseminated among bloodstain pattern analysts. Respondents (n = 32, divided into five experience levels) classified the bloodstains differently based upon amounts of training and experience, the pattern type, and the substrate type. The ability to accurately classify the specific pattern type overall (p-value < 0.0002) as wells as on fabric (p-value < 0.001) was dependent upon respondent experience level. The more experienced respondents were less likely to specifically classify patterns than the less experienced respondents (p-value < 0.001). Furthermore, the ability to accurately classify the specific pattern type was dependent upon whether the pattern had been created on fabric or some other substrate (p-value < 0.0000000001). However, if the proper morphology was present in the stain, it was found that BPA on certain fabrics was possible. This finding supports the need for further research and the development of guidelines and standards for bloodstain pattern analysis on fabric materials.
由于织物等吸收性表面上的血迹模式分析(BPA)比在坚硬、无孔表面上的应用要复杂得多,因此不同织物基材和经验丰富或经验不足的分析人员对模式类型的解释也可能不同。我们在七种织物(棉、缎纹聚酯、人造丝、混纺聚酯/氨纶、混纺尼龙/氨纶、混纺莫代尔/涤纶/氨纶和毛圈布)、泡沫板或肉纸上绘制了四种血迹图案类型(撞击飞溅、转移、滴渍和脱落),然后进行拍照。我们编制了一份调查问卷,要求受访者对 16 种图案的一般图案类型、特定图案类型和方向性进行分类,并向血迹图案分析人员分发。受访者(n = 32,分为五个经验等级)根据培训和经验的多少、图案类型和基质类型对血迹进行了不同的分类。受访者准确分类特定图案类型(p 值为 0.0002)和织物(p 值为 0.001)的能力取决于受访者的经验水平。与经验较少的受访者相比,经验较多的受访者不太可能对图案进行具体分类(p 值为 0.001)。此外,准确划分图案类型的能力还取决于图案是在织物上还是在其他基质上绘制的(p 值为 0.0000000001)。不过,如果污渍中存在适当的形态,则可以发现某些织物上可能含有双酚 A。这一发现支持了进一步研究和制定织物材料血迹图案分析指南和标准的必要性。
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引用次数: 0
Missing persons of post-conflict countries: Assessing dental identification through a questionnaire-based study in Syria 冲突后国家的失踪人员:通过在叙利亚开展基于问卷的研究评估牙齿识别情况
Q3 Medicine Pub Date : 2024-11-12 DOI: 10.1016/j.fsir.2024.100393
Rawad Qaq , Scheila Mânica , Sean McAleer
Locating and identifying missing persons is crucial to protecting human rights and rebuilding war-torn countries like Syria. Biological identity primarily relies on DNA, fingerprints, or dentition. However, post-conflict countries with high deaths, limited databases, insufficient resources, and a reduced or a lack of forensic expertise make this effort more challenging. Teeth are a practical and cost-effective means to identify human remains, particularly when acceptable dental records are available. Still, the application of comparative dental analysis in conflict scenarios needs to be more explored.
A survey study in Syria examined whether post-conflict countries could employ comparative dental identification of missing individuals. The attitudes and perceptions of four groups (the public and dentists, forensic odontologists, dental schools, and the Syrian Dental Association) were determined using questionnaires and analysed for the first two groups. Most respondents (>90 %) indicated that addressing disappearance is crucial for post-conflict reconstruction and considered it unachievable without international support and only 20 % had confidence in this support. Issues hindering human identification reported by a high percentage of dentists and forensic odontologists and included education in Forensic Odontology (92 %), expertise in the field (70 %), awareness (90 %), the availability of antemortem records (92.3 %) and political and security influence (50 %). Responses from over 80 % of the public, 50 % of the dentists and 92 % of the forensic odontologists indicated that comparative dental identification in Syria may not be feasible.
The results of this study provide strong evidence of the challenges in identifying missing persons using teeth in developing nations, particularly those affected by conflicts. They include the lack of antemortem records and forensic expertise. The international community is urged to support post-conflict nations including Syria.
定位和识别失踪人员对于保护人权和重建叙利亚这样饱受战争蹂躏的国家至关重要。生物身份识别主要依靠 DNA、指纹或牙齿。然而,冲突后国家死亡人数众多、数据库有限、资源不足、法医专业知识减少或缺乏,使得这项工作更具挑战性。牙齿是鉴定遗骸的一种实用且具有成本效益的方法,尤其是在有可接受的牙科记录的情况下。在叙利亚进行的一项调查研究考察了冲突后国家是否可以对失踪人员进行比较牙科鉴定。通过问卷调查确定了四个群体(公众和牙科医生、法医牙科专家、牙科学校和叙利亚牙科协会)的态度和看法,并对前两个群体进行了分析。大多数受访者(90%)表示,解决失踪问题是冲突后重建的关键,并认为没有国际支持是无法实现的,只有 20% 的受访者对国际支持有信心。很大比例的牙医和法医牙科医师报告了阻碍人类身份鉴定的问题,包括法医牙科教育(92 %)、该领域的专业知识(70 %)、认识(90 %)、尸体记录的可用性(92.3 %)以及政治和安全影响(50 %)。超过 80% 的公众、50% 的牙医和 92% 的法医牙科专家的回答表明,在叙利亚进行牙齿对比鉴定可能不可行。这项研究的结果有力地证明了在发展中国家,特别是受冲突影响的国家,利用牙齿鉴定失踪人员身份所面临的挑战。这项研究的结果有力地证明了在发展中国家,尤其是受冲突影响的国家,利用牙齿识别失踪人员身份所面临的挑战,其中包括缺乏死前记录和法医专业知识。我们敦促国际社会为包括叙利亚在内的冲突后国家提供支持。
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引用次数: 0
Technical Report A light touch; Reviewing the relative effectiveness of light sources and physical/chemical techniques for fingermark retrieval in casework 技术报告:轻触;审查光源和物理/化学技术在个案工作中提取指痕的相对有效性
Q3 Medicine Pub Date : 2024-11-09 DOI: 10.1016/j.fsir.2024.100391
Nicholas Marsh , Karen Marsh , Lisa Hall , Ruth Buckley , Stephen Bleay
This study reviewed the efficacy of non-contact light source examinations of serious crime scenes using ultraviolet A (UVA) light, bespoke white light and lasers, compared with subsequent powdering or chemical enhancements. Twenty-six examinations of serious crime scenes or motor vehicles were analysed, from which 655 areas of retrieved fingermark or friction ridge detail1 where reported on. It was found that undertaking a light source examination revealed equivalent numbers of marks to aluminium powder and chemical enhancements, with only a 4 % duplication rate between optical and physical/chemical techniques suggesting that both approaches are supplementary and should be used sequentially to each other.
本研究审查了使用紫外线 A (UVA)、定制白光和激光对严重犯罪现场进行非接触式光源检查的效果,并与随后的粉末处理或化学强化进行了比较。对 26 个严重犯罪现场或机动车辆进行了分析,从中报告了 655 个区域的指痕或摩擦纹细节1。结果发现,光源检查所发现的痕迹数量与铝粉和化学增强技术相当,光学技术和物理/化学技术的重复率仅为 4%,这表明这两种方法都是辅助性的,应依次使用。
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引用次数: 0
From death to the Virgin birth: Multi-analytical investigation of a suspected 16th century painting 从死亡到圣母诞生对一幅疑似 16 世纪绘画的多重分析调查
Q3 Medicine Pub Date : 2024-11-07 DOI: 10.1016/j.fsir.2024.100392
Michelle G. Carlin , Nicola Grimaldi
A painting depicting St John the Baptist prior to his brutal execution, until recently, was held in storage at the Bowes Museum in the North of England, UK. Owing to weakened joins in the wood panel the painting was marked for art conservation treatments, examination and analysis. However, other than the fact that the records show it purchased in Italy in the late nineteenth century by museum founders John and Joséphine Bowes, little else is known of the work. Through a combination of chemical testing and technical art history, further knowledge of the painting has been established. Preparation of cross sections of paint was carried out, as well as chemical testing involving pyrolysis gas chromatography – mass spectrometry (py-GCMS) and attenuated total reflectance – Fourier transform infrared spectroscopy (ATR-FTIR), lead white, chalk and linseed oil as binder were identified. The presence of an aged natural resin on the surface of the painting, presence of a thick gesso ground layer, as well as examination of the wood panel construction provided insight into the techniques used in the artwork and that they are comparable with late Medieval to early Renaissance period methods. During examination of the cross-section images, it was noted that there was a second preparatory ground layer in the central part of the sequence; this feature prompted further multi-spectral photography and analysis. X-radiography a commonly used technique for painting analysis surprisingly revealed an entirely different image depicting a nativity scene which had been previously completely concealed by the painting of St John we see today. Although an unexpected discovery still presents some unanswered questions such as who the original artist was and why was the panel so radically reworked. This investigation began with the depiction of the imminent death of the St John the Baptist and ended with the virgin birth. This analytical methodology was applied in technical art history but may also have applications in forensic investigations of suspected fraudulent works of art.
直到最近,一幅描绘施洗者圣约翰被残忍处死前的画作一直保存在英国英格兰北部的鲍斯博物馆。由于木板的接合处受损,这幅画被标记为需要进行艺术保护处理、检查和分析。然而,除了记录显示博物馆创始人约翰和约塞芬-鲍斯于 19 世纪末在意大利购买了这幅画之外,人们对这幅作品知之甚少。通过化学测试和技术艺术史的结合,我们对这幅画有了进一步的了解。我们制备了油漆的横截面,并通过热解气相色谱-质谱法(py-GCMS)和衰减全反射-傅立叶变换红外光谱法(ATR-FTIR)进行了化学测试,确定了作为粘合剂的铅白、白垩和亚麻籽油。画作表面存在老化的天然树脂、厚厚的石膏粉底层,以及对木板结构的检查,让我们了解到艺术品中使用的技术,这些技术可与中世纪晚期至文艺复兴早期的方法相媲美。在检查横截面图像时,我们注意到在序列的中央部分有第二层准备地面层;这一特征促使我们进一步进行多光谱摄影和分析。X 射线摄影是一种常用的绘画分析技术,它出人意料地发现了一幅完全不同的图像,描绘的是耶稣诞生的场景,而这幅图像之前完全被我们今天看到的圣约翰画作所掩盖。尽管这一发现出乎意料,但仍然存在一些未解之谜,例如原作者是谁,为什么这幅画会被如此彻底地重新加工。这项调查从描绘施洗者圣约翰濒临死亡开始,以童贞女降生结束。这种分析方法适用于技术艺术史,但也可用于对涉嫌欺诈的艺术品进行法医调查。
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引用次数: 0
Medico-legal analysis of the correlation between head trauma and aneurysm rupture: A case report 头部外伤与动脉瘤破裂之间相关性的医学法律分析:病例报告
Q3 Medicine Pub Date : 2024-10-18 DOI: 10.1016/j.fsir.2024.100390
Emanuele Capasso, Gaetano Di Donna, Roberto Cortese, Verdiana De Caro, Fabio Policino
We present the case of a 54 years old man who was found in a state of confusion and a forehead wound. Transported to the closest E.R., a brain CT scan showed massive subarachnoid hemorrhage and an angiographic exam showed a ruptured aneurysm of the cavernous siphon. Death occurred 4 days after admission. The autopsy showed the presence of a linear wound on the forehead which was considered a sharp force injury. The aneurysm rupture was therefore considered as a consequence of the head trauma associated to the frontal wound. In the medico-legal analysis of the case primary importance was given to the distinction between potential homicide, suicide or accident. In the end, based on the morphological features of the forehead wound, this case was defined as of potentially homicidal nature, so that the responsible of stab wound should be considered accountable for the man’s death.
本病例是一名 54 岁的男性,被发现时神志不清,前额有伤口。送往最近的急诊室后,脑部 CT 扫描显示大量蛛网膜下腔出血,血管造影检查显示海绵虹吸管动脉瘤破裂。患者在入院 4 天后死亡。尸检显示前额有一条线状伤口,被认为是锐器所伤。因此,动脉瘤破裂被认为是与前额伤口相关的头部外伤造成的。在对该病例进行医学-法律分析时,最重要的是区分可能是他杀、自杀还是意外事故。最后,根据前额伤口的形态特征,该病例被界定为潜在的他杀性质,因此刺伤者应被视为该男子死亡的罪魁祸首。
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引用次数: 0
Management and disposal of human remains acquired for medical education – A forensic perspective 管理和处置为医学教育获取的遗骸--法医学视角
Q3 Medicine Pub Date : 2024-10-11 DOI: 10.1016/j.fsir.2024.100389
Neelkamal Battu , Rutuja Kanekar , Siddharth Sawardekar , Girish Tasgaonkar, Vikrant Waghmare
In medical schools, the foundational subject of human anatomy usually requires the medical students to procure a set of human bones, either real or artificial, for comprehensive understanding of the human body. The proper maintenance and disposal of these human remains is the responsibility of the students and the institutions possessing the remains. Improper disposal of human remains can have far-reaching implications, including ethical, legal, public health, environmental, social, and psychological consequences. We present a case of a human skull used for medical studies found dumped in a garbage, leading to unnecessary investigation by the law authorities. The case brings to light the need for including ethical disposal of academic human skeletons at the beginning of the medical curriculum. The authors also discuss in detail the various legal provisions and cultural practices of disposal of human remains in the Indian scenario.
在医学院,人体解剖学这门基础学科通常要求医科学生购买一套真实或人造的人体骨骼,以便全面了解人体。妥善保管和处置这些人体遗骸是学生和拥有遗骸的机构的责任。人类遗骸处置不当会产生深远的影响,包括伦理、法律、公共卫生、环境、社会和心理后果。我们介绍了一个案例,一个用于医学研究的人类头骨被发现丢弃在垃圾中,导致法律部门进行了不必要的调查。这个案例让人们认识到,在医学课程的开始阶段,就有必要将学术研究用的人类骸骨的道德处置纳入其中。作者还详细讨论了印度处理人类遗骸的各种法律规定和文化习俗。
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引用次数: 0
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Forensic Science International: Reports
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