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Legal and ethical breaches in personal health data handling among cardiologists: A multicenter survey on compliance gaps and forensic risk 心脏病专家个人健康数据处理中的法律和道德违规:一项关于合规差距和法医风险的多中心调查
Q3 Medicine Pub Date : 2025-12-23 DOI: 10.1016/j.fsir.2025.100444
Ufuk İyi̇gün , Deniz Ergene

Background

The digitization of healthcare services in Türkiye has introduced significant legal and ethical challenges, particularly concerning the protection of personal health data. Despite established legal frameworks, limited empirical data exist regarding the behavioral and legal awareness of physicians, especially cardiologists, in complying with data protection regulations.

Objective

To evaluate the legal and practical awareness of cardiologists in Türkiye regarding the protection of personal health data, identify common non-compliant practices, and assess associated forensic risks.

Methods

A cross-sectional, multicenter survey was conducted with 52 cardiologists from various institutions in Türkiye. The questionnaire, developed through literature review and expert input, included 12 items addressing clinical data practices, password sharing, anonymization awareness, and communication methods. Descriptive statistical analysis was performed using SPSS v26.

Results

The majority of respondents demonstrated poor compliance with core data protection principles. While 71 % were aware of legal sanctions, nearly half admitted to sharing patient data without consent, and 78.85 % used unsecured platforms like WhatsApp for data transmission. Moreover, 67.31 % were unfamiliar with anonymization, and only one-third maintained exclusive control over their digital signatures.

Conclusions

This study highlights a critical gap between legal knowledge and clinical practice among cardiologists in Türkiye. Institutional interventions—including targeted training, legal education, and secure data systems—are urgently needed to improve compliance, mitigate forensic risk, and uphold patient privacy.
背景:土耳其医疗保健服务的数字化带来了重大的法律和道德挑战,特别是在保护个人健康数据方面。尽管建立了法律框架,但关于医生,特别是心脏病专家在遵守数据保护法规方面的行为和法律意识方面的经验数据有限。目的评估土耳其心脏病专家在个人健康数据保护方面的法律和实践意识,识别常见的不合规做法,并评估相关的法医风险。方法采用横断面、多中心调查方法,对来自日本各机构的52名心脏病专家进行调查。该问卷通过文献综述和专家意见制定,包括12个项目,涉及临床数据实践、密码共享、匿名意识和沟通方法。采用SPSS v26进行描述性统计分析。结果大多数受访者对核心数据保护原则的遵守程度较差。虽然71% %的人知道法律制裁,但近一半的人承认未经同意分享患者数据,78.85% %的人使用WhatsApp等不安全的平台进行数据传输。此外,67.31 %的人不熟悉匿名化,只有三分之一的人对他们的数字签名保持独家控制。结论:这项研究突出了法律知识与临床实践之间的关键差距在泰国心脏病专家。迫切需要机构干预,包括有针对性的培训、法律教育和安全的数据系统,以提高合规性,降低法医风险,并维护患者隐私。
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引用次数: 0
Descriptive quality of traumatic lesion documentation in emergency medical certificates 急诊诊断书中创伤损伤记录的描述质量
Q3 Medicine Pub Date : 2025-12-23 DOI: 10.1016/j.fsir.2025.100446
Khedija Zaouche , Hana Harzallah , Emna Rezgui , Emna Kallel , Rania Hmaissi , Wicem Hannachi , Fedya El Ayech , Manel Kallel

Purpose

The Initial Medical Certificate (IMC) is a crucial medico-legal document, particularly in cases of physical violence, where its descriptive accuracy directly influences its evidentiary value in legal and compensation processes. This study aimed to assess the quality of traumatic lesion documentation in IMCs issued in emergency departments and to identify common deficiencies.

Methods

We conducted a retrospective descriptive study between July and September 2022 in the emergency department of a regional hospital in Tunisia. IMCs issued following physical assault or road traffic accidents were included. Evaluation focused on five common lesion types (erythema, ecchymosis, hematoma, excoriation, wound) using standardized criteria: size, shape, color, depth, location, and estimated age. A binary scoring system classified documentation quality as good, average, or poor.

Results

Out of 218 IMCs analyzed, 54.6 % involved female patients with a mean age of 37 years. Judicial requisitions prompted 94.4 % of the certificates. Descriptions were rated as poor in 47.2 % of cases and average in 52.8 %. No certificate included anatomical landmarks. Wounds were the best described (p < 0.001), while ecchymoses were more often poorly documented (p = 0.016). Lesion size (p < 0.001) and depth (p = 0.02) were the most frequently reported elements.

Conclusion

Significant deficiencies were found in the documentation of elementary lesions, compromising the medico-legal value of IMCs. Implementing structured reporting tools and ongoing training is essential to improve descriptive quality and the probative strength of these certificates.
目的初步医疗证明是一份至关重要的医学法律文件,特别是在身体暴力案件中,其描述的准确性直接影响其在法律和赔偿程序中的证据价值。本研究旨在评估急诊科发布的IMCs创伤性病变记录的质量,并找出常见的缺陷。方法:我们于2022年7月至9月在突尼斯一家地区医院的急诊科进行回顾性描述性研究。人身攻击或道路交通事故后发出的综合影响评估报告也包括在内。评估集中在五种常见的病变类型(红斑、瘀斑、血肿、擦伤、伤口),使用标准化标准:大小、形状、颜色、深度、位置和估计年龄。二进制评分系统将文档质量分为好、一般或差。结果在分析的218例IMCs中,54.6% %涉及平均年龄为37岁的女性患者。司法征用促使94.4 %的证书。描述差的病例占47.2% %,平均的病例占52.8% %。没有证明包括解剖标志。伤口的描述最好(p <; 0.001),而瘀斑的记录往往较差(p = 0.016)。病变大小(p <; 0.001)和深度(p = 0.02)是最常报道的因素。结论原发性病变文献存在明显缺陷,影响了IMCs的法医学价值。实施结构化报告工具和持续培训对于提高这些证书的描述质量和证明强度至关重要。
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引用次数: 0
Fatal injury from tire explosion during disassembly: A forensic case report 拆轮胎爆炸致人死亡:一份法医案例报告
Q3 Medicine Pub Date : 2025-12-19 DOI: 10.1016/j.fsir.2025.100445
Shota Furukawa , Ako Sasao , Yuki Ohtsu , Hiroshi Tsutsumi , Chihiro Kawai , Masashi Miyao , Rie Sano , Yoko Nishitani
Cases of fatality caused by a tire explosion are extremely rare, especially during the disassembly process. Tire explosions can occur due to improper techniques during assembly or maintenance; however, serious accidents can also occur during disassembly due to the high-pressure air remaining inside the tire tube, as in this case. We present the case of a man in his 60 s who died after being struck by a tire rim due to a tire explosion during the dismantling of a large wheel and tire set of an airplane passenger boarding bridge. Autopsy revealed complex fractures of the head and chest, fractures of the thoracic vertebrae and upper extremities, and damage to multiple organs. A characteristic feature of this case is thoracic vertebral fracture, which has not been found in previous fatal tire explosion cases, suggesting that the injury resulted from a very large external force. This indicates that discarded tires still contain enough pressurized air to launch the rim, posing a danger equal to or greater than that of tire explosions during assembly or maintenance. This case highlights the importance of safety measures during tire disassembly. Although it is mandatory to use a safety cage to fill the tire with air during maintenance, no such safety measures were undertaken during disassembly. It is necessary to implement safety measures and provide equipment in the workplace to protect the workers.
轮胎爆炸造成死亡的案例极为罕见,特别是在拆卸过程中。在装配或维修过程中,由于技术不当,可能会发生轮胎爆炸;然而,在拆卸过程中,由于高压空气留在轮胎内胎内,也可能发生严重的事故,就像这次一样。我们提出了一个60多岁的男子,他在拆除飞机乘客登机桥的一个大轮子和轮胎组时,因轮胎爆炸而被轮胎圈击中而死亡。尸检显示头部和胸部复杂骨折,胸椎和上肢骨折,多个器官受损。本病例的一个特点是胸椎骨折,这在以往致命的轮胎爆炸病例中没有发现,提示损伤是由非常大的外力造成的。这表明丢弃的轮胎仍然含有足够的加压空气来启动轮辋,造成的危险等于或大于轮胎在组装或维修期间爆炸的危险。这个案例突出了轮胎拆卸过程中安全措施的重要性。虽然在维护过程中必须使用安全笼填充轮胎,但在拆卸过程中没有采取此类安全措施。有必要在工作场所实施安全措施并提供设备来保护工人。
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引用次数: 0
Forensic reconstruction of an incident scene using rigid body photogrammetry techniques 使用刚体摄影测量技术对事故现场进行法医重建
Q3 Medicine Pub Date : 2025-12-15 DOI: 10.1016/j.fsir.2025.100443
Kevin Gilmore , Geoffrey T. Desmoulin , Szymon Claridad , Marc-André Nolette , Theodore E. Milner
Forensic biomechanics is used to draw conclusions about incident and injury reports, relying on images of the incident for relevant photogrammetric measurement techniques, such as spatial resection and intersection. However, these techniques rely on the quality and type of media available, which can vary substantially. As such, this study aims to quantify the error associated with utilizing various supporting media. A simulated incident scene containing 2 objects of interest, a model rifle and a motorcycle, was 3D scanned and recorded from 3 camera angles. PhotoModeler was used to measure the 3D location of these objects with supporting media being limited to the use of a 3D scan, calibrated or uncalibrated cameras, single or multiple viewing angles, and stationary or moving cameras. The results of statistical analysis demonstrated that, when supported by a scan, single and multiple camera angles resulted in similar positional measurement errors. Mean errors of 6.52 cm and 5.98 cm for the single view, compared to ranges of 3.73–5.71 cm and 2.56 – 13.74 cm with multiple views, were found for the motorcycle and rifle, respectively. Also, using 3 stationary cameras resulted in lower distance and orientation errors than 3 frames from a moving camera. Thus, it was concluded that supporting 3D scans provide the highest level of accuracy and the use of single or multiple stationary cameras demonstrated higher accuracy compared to mobile cameras. Using 3D scans in conjunction with stationary cameras provides reliability and admissibility of photogrammetry-based evidence in forensic investigations.
法医生物力学是利用事件和伤害报告得出结论,依靠事件的图像进行相关的摄影测量技术,如空间切除和相交。然而,这些技术依赖于可用媒体的质量和类型,这可能有很大差异。因此,本研究旨在量化与使用各种支持媒体相关的误差。模拟的事件现场包含两个感兴趣的物体,一个模型步枪和一辆摩托车,从3个摄像机角度进行3D扫描和记录。使用PhotoModeler来测量这些物体的3D位置,支持媒体仅限于使用3D扫描,校准或未校准的相机,单个或多个视角,以及静止或移动的相机。统计分析结果表明,在扫描支持下,单个和多个相机角度导致相似的位置测量误差。摩托车和步枪的平均误差分别为3.73-5.71 cm和2.56 - 13.74 cm,而单视图的平均误差分别为6.52 cm和5.98 cm。此外,使用3个固定摄像机比使用3个移动摄像机产生更低的距离和方向误差。因此,得出的结论是,支持3D扫描提供了最高水平的精度,使用单个或多个固定相机与移动相机相比,显示出更高的精度。在法医调查中,将3D扫描与固定摄像机结合使用可提供基于摄影测量的证据的可靠性和可采性。
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引用次数: 0
Criminal use of carbamazepine: A case of drug-facilitated captivity in Lubumbashi, DR Congo 卡马西平的犯罪使用:刚果民主共和国卢本巴希的一起毒品促成的囚禁案例
Q3 Medicine Pub Date : 2025-12-09 DOI: 10.1016/j.fsir.2025.100441
Paul Musa Obadia , Elodie Lwendela Kalasa , Georges Kalenga Ilunga , Tony Kayembe-Kitenge , O. Inès , Margot De Vis , Jan Tytgat , Daniel Okitundu Luwa E-Andjafono , Célestin Banza Lubaba Nkulu , Benoit Nemery
A man was kidnapped and then drugged during a 5-day captivity. After his release, he exhibited various neurological symptoms (inability to walk independently, drowsiness, difficulties to speak, dizziness, headache and a dry mouth). Toxicological analyses of blood and urine samples obtained 3 days after his release revealed therapeutic levels of carbamazepine and its active metabolite in both blood and urine, along with metronidazole and nicotinamide. Carbamazepine has hitherto not been implicated in drug-related crimes. This case highlights the potential misuse of easily available therapeutic drugs for criminal purposes in low-income countries and illustrates the value of prompt international collaboration to elucidate etiologies in forensic toxicology.
一名男子在5天的囚禁中被绑架并下药。释放后,他表现出各种神经系统症状(不能独立行走、嗜睡、说话困难、头晕、头痛和口干)。释放3天后对血液和尿液样本进行毒理学分析,结果显示血液和尿液中卡马西平及其活性代谢物、甲硝唑和烟酰胺均有治疗水平。迄今为止,卡马西平尚未与毒品有关的犯罪有牵连。该案例突出了低收入国家容易获得的治疗药物可能被滥用于犯罪目的,并说明了迅速开展国际合作以阐明法医毒理学病因的价值。
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引用次数: 0
DNA barcoding for the identification of swim bladders: An approach to international trade monitoring 鱼鳔DNA条形码鉴定:国际贸易监测的一种方法
Q3 Medicine Pub Date : 2025-12-05 DOI: 10.1016/j.fsir.2025.100440
Gabriela Idaline de Freitas , Carlos E. Rodrigues Jr , Daniel Eduardo Visciano de Carvalho , Ricardo Utsunomia , Fabio Porto-Foresti
The rapidly expanding global trade in fish maw (dried swim bladders) necessitates urgent forensic monitoring, as this highly processed commodity is morphologically unidentifiable, directly facilitating the illicit inclusion of vulnerable species in the legal supply chain. This research applied DNA Barcoding (cytochrome oxidase I; COI gene) to 120 fish maw samples seized by Brazilian environmental authorities at a major international airport to provide species-level identification and crucial data on trade practices. We identified eight species, with the overwhelming majority belonging to Amazonian Sciaenids (Cynoscion acoupa and Plagioscion auratus) and the catfish Sciades parkeri. Crucially, the definitive identification of C. acoupa and S. parkeri confirms the direct exploitation of species listed as Vulnerable by the IUCN, tracing their flow from multiple Brazilian states to major global consumer hubs, including Hong Kong, the United States, United Kingdom and China. Furthermore, the study exposed a critical methodological constraint: an initial ambiguous species assignment by the BOLD system (94 % match) was only resolved by confirming the correct species, S. parkeri, through the NCBI database (100 % match). This finding demonstrates the essential need for critical evaluation and the utilization of complementary reference libraries to overcome 'code gaps' in forensic analyses. These data underscore the essential role of molecular techniques as an enforcement tool for traceability and provide unequivocal evidence supporting the immediate need for targeted legislation and rigorous regulatory oversight to protect vulnerable Amazonian stocks.
鱼鳔(干鱼鳔)的全球贸易迅速扩大,需要紧急的法医监测,因为这种高度加工的商品在形态上无法识别,直接促进了将脆弱物种非法纳入合法供应链。本研究将DNA条形码(细胞色素氧化酶I; COI基因)应用于巴西环境当局在一个主要国际机场查获的120份鱼鳔样本,以提供物种水平的鉴定和贸易实践的关键数据。我们发现了8种,绝大多数属于亚马逊的Sciaenids (Cynoscion coupa和Plagioscion auratus)和Sciades parkeri鲶鱼。至关重要的是,对coupa和S. parkeri的明确鉴定证实了对IUCN列为易危物种的直接开发,追踪了它们从巴西多个州流向包括香港、美国、英国和中国在内的主要全球消费中心。此外,该研究暴露了一个关键的方法约束:最初由BOLD系统(94 %匹配)进行的模糊物种分配只有通过NCBI数据库(100 %匹配)确认正确的物种parkeri才能解决。这一发现表明了对批判性评估和利用补充参考库来克服法医分析中的“代码缺口”的基本需求。这些数据强调了分子技术作为可追溯性执法工具的重要作用,并提供了明确的证据,支持迫切需要有针对性的立法和严格的监管监督,以保护脆弱的亚马逊种群。
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引用次数: 0
Delta-8-THC acetate-a prodrug of delta-8-THC?: A case report δ -8- thc乙酸- δ -8- thc的前药?:病例报告
Q3 Medicine Pub Date : 2025-12-01 DOI: 10.1016/j.fsir.2025.100435
Jonathan Wong , Evelyn Mei Ling Goh , Jia Ying Goh , Mei Ching Ong , Hooi Yan Moy , Angeline Tiong Whei Yap
This case report describes the concurrent analysis of a seized plant material and a urine sample. The seized plant material was found to contain delta-8-THC acetate, a prodrug of delta-8-THC, as a major component. While the urine sample was found to contain metabolites of delta-8-THC, this was disparate with the relative abundance of delta-8-THC detected in the plant material recovered from the suspect. Delta-8-THC acetate in the plant material was fully characterised by GC-MS, LC-Orbitrap-MS and NMR. Metabolism studies using human liver microsomes demonstrated metabolism of delta-8-THC acetate with the concurrent formation of delta-8-THC-COOH as the primary metabolite. The in-vitro findings were consistent with the authentic urine sample, suggesting that detection of delta-8-THC-COOH likely resulted from delta-8-THC-acetate consumption. This work demonstrates the critical role of orthogonal analytical techniques in the analysis of controlled substances and emphasises the necessity of accounting for prodrug metabolism when interpreting results from the analysis of urine samples.
本病例报告描述了对查获的植物材料和尿液样本的同时分析。查获的植物材料含有δ -8-四氢大麻酚乙酸酯,这是δ -8-四氢大麻酚的前药,是主要成分。虽然发现尿液样本中含有δ -8-四氢大麻酚的代谢物,但这与从嫌疑人身上回收的植物材料中检测到的δ -8-四氢大麻酚的相对丰度不同。采用GC-MS、LC-Orbitrap-MS和NMR对植物材料中的δ -8- thc乙酸酯进行了表征。利用人肝微粒体进行的代谢研究表明,δ -8- thc醋酸酯的代谢与δ -8- thc - cooh的同步形成是主要代谢产物。体外实验结果与真实尿样一致,表明δ -8- thc - cooh的检测可能是由于δ -8- thc -乙酸盐消耗所致。这项工作证明了正交分析技术在受控物质分析中的关键作用,并强调了在解释尿样分析结果时考虑药物前代谢的必要性。
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引用次数: 0
Differentiation between bullet types by means of a 3D analysis of gunshot cavitation and bullet fragmentation - A computed tomography study in ballistic gelatine 通过射击空化和子弹破片的三维分析来区分子弹类型——弹道明胶的计算机断层扫描研究
Q3 Medicine Pub Date : 2025-12-01 DOI: 10.1016/j.fsir.2025.100436
Juho-Antti Junno , Mikael Brix , Eveliina Lammentausta , Alina Junno , Timo Liimatainen , Jaakko Niinimäki , Juha Kiljunen , Petteri Oura
Identification of the projectile type is among the main objectives in the forensic investigation of a gunshot incident, even if the projectile itself cannot be recovered. Medical imaging, primarily computed tomography (CT), has become a valuable tool in the examination and documentation of gunshot injuries. Bullet types often exhibit distinct patterns of fragment deposition and wound cavitation. In this study, we examined the potential of a CT-based digital three-dimensional (3D) analysis of cavitation and fragmentation patterns to distinguish between four bullets of.223 Remington ammunition (one soft point, one full metal jacket, and two expanding monolithic bullets). We utilized 20 x 20 x 25 cm blocks of ballistic gelatine as tissue simulant, with artificial skin on entry and exit walls of the blocks. Test firing was performed from a distance of 50 m. The gelatine blocks were CT-scanned and 3D reconstructions of the blocks, demonstrating both air cavitation and metal fragments, were generated. In addition, other parameters of cavitation and fragmentation were obtained from the CT scans, including the volume and internal surface area of the air cavity within the gelatine block. Entry and exit defects of the skin simulant were also documented. Our results indicated that different bullet types exhibited different cavitation and fragmentation patterns. We also detected differences in exit wounds in the skin simulant. Further studies are needed to confirm our preliminary findings that a digital 3D analysis may aid to distinguish the bullet type in certain forensic scenarios.
在枪击事件的法医调查中,识别弹丸类型是主要目标之一,即使弹丸本身无法恢复。医学成像,主要是计算机断层扫描(CT),已成为检查和记录枪伤的宝贵工具。子弹类型通常表现出明显的碎片沉积和伤口空化模式。在这项研究中,我们研究了基于ct的空化和破片模式的数字三维(3D)分析的潜力,以区分四颗。223子弹雷明顿弹药(一枚软弹,一枚全金属弹套,两枚膨胀整体式子弹)。我们使用20 x 20 x 25 cm的弹道明胶块作为组织模拟物,在块的入口和出口壁上放置人造皮肤。试验射击距离为50 m。对明胶块进行了ct扫描,并生成了三维重建块,显示了空气空化和金属碎片。此外,从CT扫描中获得了其他空化和破碎参数,包括明胶块内空气腔的体积和内表面积。皮肤模拟物的入口和出口缺陷也被记录下来。结果表明,不同类型的子弹具有不同的空化破片模式。我们还在皮肤模拟物中发现了出口伤口的差异。需要进一步的研究来证实我们的初步发现,即数字3D分析可能有助于在某些法医场景中区分子弹类型。
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引用次数: 0
From battlefield to burial: Complex casualty identification using rapid DNA analysis in 21st century warfare 从战场到埋葬:21世纪战争中使用快速DNA分析的复杂伤亡鉴定
Q3 Medicine Pub Date : 2025-12-01 DOI: 10.1016/j.fsir.2025.100439
Dan M. Weinthal , Amit Blumberg , Elisha Ziv , Hillel Shuman , Danielle Waingrod , Reouven Berdugo , David Shore , Noam Shomron , Alon Oz
On October 7, 2023, terror organizations launched a large-scale surprise attack on Israel, resulting in the deadliest mass fatality incident in the nation’s history. The assault left 1438 Israelis dead, 255 taken hostage, and over 1600 assailants killed in defensive operations. In the immediate aftermath, the Israel Defense Forces (IDF) Genomic Center for Casualties Identification rapidly expanded its capacity by integrating rapid DNA technology and implementing refined sample prioritization protocols to accelerate the identification process. This mass fatality incident unfolded under unprecedented circumstances. Thousands of human remains, often fragmented, burned, or intermingled with those of perpetrators, were recovered across multiple sites. Conventional identification methods such as dental or fingerprint analysis were frequently unavailable, leaving DNA profiling the primary method of identification. The extreme scale and severity of the event meant that each case presented unique combination of sample types, often requiring repeated extractions and multiple testing approaches to obtain viable genetic profiles. These challenges, though described here in scientific terms, reflected the reality of a brutal attack that left families anxiously awaiting for closure, and forensic teams working around the clock in conditions rarely seen in civilian laboratories. Our findings indicate that while rapid DNA profiling enabled identifications in under three hours, the key determinant of timely results was precise sample triage and prioritization. We therefore propose a combined approach integrating strategic triage with fast-track DNA profiling to manage complex casualty events effectively. Using this approach, all casualties within our ground operations were identified within 2–3 h. Beyond the technical achievements, this experience underscores the dual mission of forensic science during national crisis: to uphold the highest standard of accuracy and integrity, while performing this work with humanity and respect amidst profound loss. The lessons learned from October 7th, much like those drawn from 9/11 and the long-term work of the International Commission on Missing Persons (ICMP), which investigates mass graves and missing persons from conflicts and disasters, provide valuable guidance for forensic agencies worldwide, offering strategies to strengthen preparedness and resilience for future mass fatality incidents.
2023年10月7日,恐怖组织对以色列发动大规模突然袭击,造成以色列历史上最严重的集体死亡事件。这次袭击造成1438名以色列人死亡,255人被劫持为人质,1600多名袭击者在防御行动中丧生。在事故发生后不久,以色列国防军(IDF)伤亡鉴定基因组中心通过整合快速DNA技术和实施精细的样本优先排序协议,迅速扩大了其能力,以加速鉴定过程。这起大规模死亡事件是在前所未有的情况下发生的。在多个地点发现了数千具人类遗骸,这些遗骸通常是破碎的、烧毁的,或者与肇事者的遗骸混在一起。传统的鉴定方法,如牙齿或指纹分析,往往是不可用的,留下DNA分析的主要鉴定方法。该事件的极端规模和严重性意味着每个病例都呈现出独特的样本类型组合,通常需要反复提取和多种测试方法才能获得可行的基因图谱。尽管本文用科学术语描述了这些挑战,但它们反映了残酷袭击的现实,让家属焦急地等待结束,法医小组在民用实验室罕见的条件下夜以继日地工作。我们的研究结果表明,虽然快速DNA分析能够在三小时内进行鉴定,但及时结果的关键决定因素是精确的样本分类和优先排序。因此,我们提出了一种结合战略分诊和快速DNA分析的综合方法,以有效地管理复杂的伤亡事件。使用这种方法,我们地面行动中的所有伤亡都在2-3 小时内确定。除了技术成就之外,这一经历还强调了法医学在国家危机期间的双重使命:在遭受重大损失的情况下,保持最高标准的准确性和完整性,同时以人道和尊重的态度开展这项工作。从10月7日事件中吸取的教训,就像从9/11事件中吸取的教训,以及调查冲突和灾害中万人坑和失踪人员的国际失踪人员委员会(ICMP)的长期工作一样,为世界各地的法医机构提供了宝贵的指导,提供了加强对未来大规模死亡事件的准备和复原力的战略。
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引用次数: 0
Internal validation of probabilistic genotyping software STRmix™ V2.8 for GlobalFiler™ profiles generated from Japanese individuals 概率基因分型软件STRmix™V2.8对日本个体生成的GlobalFiler™档案进行内部验证
Q3 Medicine Pub Date : 2025-11-19 DOI: 10.1016/j.fsir.2025.100437
Takashi Fukagawa, Haruhiko Watahiki, Yusuke Mita, Tetsushi Kitayama, Koji Fujii, Natsuko Mizuno
The interpretation of mixed DNA profiles originating from multiple individuals can be very challenging. Probabilistic genotyping software is used to interpret such mixed DNA profiles, and it assigns a likelihood ratio based on the evidence given two hypotheses: a prosecution hypothesis and a defense hypothesis. For proper utilization of probabilistic genotyping software, internal validation is required. In this study, we performed internal validation of the probabilistic genotyping software STRmix™. Our internal validation studies complied with the Scientific Working Group on DNA Analysis Methods guidelines for the validation of probabilistic genotyping systems, and was performed using GlobalFiler™ profiles generated from Japanese individuals to study the sensitivity, specificity, and precision of STRmix™, and the effects of the addition of a known contributor and incorrect assumption of the number of contributors were also assessed. The results of our internal validation studies suggest that STRmix™ using our laboratory-specific parameters is suitable for the purpose of interpreting mixed DNA profiles and statistical evaluation in our experimental and analytical environment. In addition, there were a few rare cases in which STRmix™ interpreted the results as exclusion (likelihood ratio = 0), despite the person of interest being a true contributor, due to extreme heterozygote imbalance and/or significant differences in the mixture ratio between loci due to the stochastic effects of PCR amplification. Therefore, we report these rare cases along with the results of our internal validation studies.
对来自多个个体的混合DNA图谱的解释可能非常具有挑战性。概率基因分型软件被用来解释这种混合的DNA图谱,它根据两个假设的证据分配一个可能性比:起诉假设和辩护假设。为了正确利用概率基因分型软件,需要进行内部验证。在本研究中,我们对概率基因分型软件STRmix™进行了内部验证。我们的内部验证研究遵循了DNA分析方法科学工作组关于概率基因分型系统验证的指南,并使用从日本个体生成的GlobalFiler™档案来研究STRmix™的敏感性、特异性和精度,并评估了添加已知贡献者和错误假设贡献者数量的影响。我们的内部验证研究结果表明,使用我们实验室特定参数的STRmix™适用于在我们的实验和分析环境中解释混合DNA图谱和统计评估。此外,在少数罕见的情况下,STRmix™将结果解释为排除(可能性比= 0),尽管感兴趣的人是一个真正的贡献者,由于极端杂合子不平衡和/或由于PCR扩增的随机效应,位点之间的混合比例存在显着差异。因此,我们报告了这些罕见的病例以及我们内部验证研究的结果。
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Forensic Science International: Reports
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