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Sex estimation and sexual dimorphism analysis through hand anthropometry: Insights from a cross-sectional study 通过手部人体测量进行性别估计和性别二形性分析:一项横断面研究的启示。
Q3 Medicine Pub Date : 2024-06-14 DOI: 10.1016/j.fsir.2024.100374
M.O. Suleiman , B. Danborno , S.A. Musa , J.A. Timbuak , A.O. Yusuf , H.O. Suleiman

One crucial aspect of a forensic procedure is sex estimation during individual identification. Hand and its remains are usually among the common body parts found in scenes of mass disasters during forensic investigations. Hand outlines are also important in several biomedical fields such as prosthetics, plastic surgery, and ergonomics. This cross-sectional study was done to investigate sexual dimorphism and discriminate sex using hand and hand print dimensions of 283 male and 317 female subjects who were randomly selected. Bare hand measurement and bilateral hand prints were obtained using Vernier caliper, inked pad, and A4 plain paper. Data obtained were analyzed using SPSS version 23.0 IBM. Sexual dimorphism in all the hand dimensions and prints of both hands were observed. The binary logistic regression model showed that in the left hand, bare left-hand breadth (BLHB) was the single best variable to discriminate sex with a percentage accuracy of 69.5 %. Bare right-hand breadth (BRHB) was the best predictor in the right hand with a percentage accuracy of 71.8 %. The predicted probabilities of the binary logistic model (BLR) for the variables that contributed best to the prediction were analyzed using the receiver operating characteristic (ROC) curve. It was observed that the BLHB and BRHB had the highest area under the curve (above 0.5) in the hand and print variables respectively. Sexual dimorphism and sex determination were established in this study using hand dimensions and prints. The BLHB and BRHB were the best sex determinants in this study. Although the accuracies of sex estimation from both hands in this study are low, it does confirm and support previous reports of handbreadth being the best variable for sex estimation.

法证程序的一个重要方面是在进行个体鉴定时对性别进行估计。在法医调查中,手及其残骸通常是在大规模灾难现场发现的常见身体部位之一。手部轮廓在假肢、整形外科和人体工程学等多个生物医学领域也很重要。这项横断面研究旨在利用随机抽取的 283 名男性和 317 名女性受试者的手部和手印尺寸来研究性双态性并区分性别。研究人员使用游标卡尺、墨水垫和 A4 普通纸对受试者的裸手和双侧手印进行了测量。获得的数据使用 SPSS 23.0 IBM 版本进行分析。观察到所有手部尺寸和双手指纹都存在性别二态性。二元逻辑回归模型显示,左手的裸左手宽(BLHB)是区分性别的最佳变量,准确率为 69.5%。裸右手宽(BRHB)是右手的最佳预测变量,准确率为 71.8%。使用接收者操作特征曲线(ROC)分析了二元逻辑模型(BLR)中对预测贡献最大的变量的预测概率。结果表明,在手和指纹变量中,BLHB 和 BRHB 的曲线下面积最高(超过 0.5)。本研究利用手的尺寸和指纹确定了性二型和性别。在这项研究中,BLHB 和 BRHB 是最好的性别决定因素。虽然本研究中通过双手估计性别的准确率较低,但它确实证实并支持了之前关于手宽是估计性别的最佳变量的报道。
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引用次数: 0
SynTissue® as a surrogate material for the human scalp SynTissue® 作为人类头皮的替代材料
Q3 Medicine Pub Date : 2024-06-13 DOI: 10.1016/j.fsir.2024.100373
Geoffrey T. Desmoulin , Marc-André Nolette , Theodore E. Milner

Synthetic skin produced by SynDaver®, currently used primarily in medical testing and training applications, may be suitable as a surrogate for human skin in forensic investigations. To determine how accurately the company's synthetic skin, SynTissue®, could mimic the mechanical properties of human skin, tests were conducted to measure its elastic modulus and resistance to laceration. Test results were compared to published data acquired from tensile tests conducted on human scalp and impacts with blunt objects on porcine heads. The stress vs strain relation for SynTissue® 8 N corresponded closely to that of the human scalp. Deformations similar to skin lacerations were observed when SynTissue® was subjected to blunt object impacts, at forces in the range of those reported for lacerations of cadaver and porcine heads. However, the published data are insufficient to unequivocally assess the suitability of SynTissue® for forensic investigations of lacerations. Moreover, there are features of the SynTissue® impact deformations that can provide useful information even if the laceration threshold turns out to be lower than that of human skin.

SynDaver®公司生产的合成皮肤目前主要用于医疗测试和培训应用,在法医调查中可能适合作为人类皮肤的替代物。为了确定该公司的合成皮肤 SynTissue® 能在多大程度上准确模拟人类皮肤的机械特性,进行了测量其弹性模量和抗撕裂性的测试。测试结果与在人类头皮上进行的拉伸测试和钝器对猪头的撞击所获得的公开数据进行了比较。SynTissue® 8 N 的应力与应变关系与人类头皮的应力与应变关系非常接近。当 SynTissue® 受到钝物撞击时,可观察到与皮肤撕裂类似的变形,其作用力与尸体和猪头部撕裂的作用力相仿。然而,已公布的数据不足以明确评估 SynTissue® 是否适合用于法医调查撕裂伤。此外,SynTissue® 冲击变形的一些特征可以提供有用的信息,即使撕裂阈值低于人类皮肤的阈值。
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引用次数: 0
Diaphragm histology in SIDS and other sudden unexplained deaths 婴儿猝死综合症和其他不明原因猝死的膈肌组织学研究
Q3 Medicine Pub Date : 2024-05-30 DOI: 10.1016/j.fsir.2024.100372
Dov Jordan Gebien

This is a Letter to the Editor in response to Kariks J. Diaphragmatic muscle fibre necrosis in SIDS. Forensic Sci Int. 1989 Dec;43(3):281–91. doi: 10.1016/0379-0738(89)90156-4. PMID: 2613142.

这是一封致编辑的信,回应 Kariks J. 婴儿猝死综合症中的膈肌纤维坏死。Doi: 10.1016/0379-0738(89)90156-4.PMID: 2613142.
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引用次数: 0
Delayed decompositional changes in indoor settings among Tibetan monastic communities in India: A case report 印度西藏寺庙社区室内环境中的延迟分解变化:病例报告
Q3 Medicine Pub Date : 2024-05-16 DOI: 10.1016/j.fsir.2024.100370
Tawni L. Tidwell , Leslie E. Eisenberg , Alexander Fedotov , Elena Kokurina , Julia Boytsova , Tenzin Desel , Lodoe Sangpo , Ngawang Norbu , Jigme Chokdrup , Dorji Tsering , Thupten Sherap , Tanzin Chhonden , Yeshi Dorje , Chhoktan Lama , Kunga Lhundup , Jamyang , Lharkyab , Lobsang Phuntsok , Tenzin Wangchuk , Stanzin Lhakpa , Svyatoslav Medvedev

Within the fields of forensic anthropology and forensic medicine in general, the ability to provide accurate estimates of time since death that minimize error, maximize precision, and consider a multitude of variables is a critical component of all forensic and medicolegal investigations that focus on the identification of human remains and the circumstances surrounding death. In comparison with many forensic cases where date of death is typically unknown, we begin with a known postmortem interval and progressively assess, through external testing measures (temperature, skin elasticity and skin color changes) followed by photography and videography, decompositional changes over time. This work seeks to provide well-described cases with documented biological parameters to propose hypotheses for future research. Field team members observe and record any decompositional changes associated with the states of algor, rigor, and livor mortis, as well as progressive changes in skin color and tissue integrity in an environment where variability in climate and ecological factors may vary from case to case. The two cases were followed for 31 (postmortem interval [PMI] 38) and 19 (PMI 27) days in humid subtropical and tropical monsoon Köppen-Geiger classified climatic zones, respectively, where delayed decompositional and putrefactive changes observed are at odds with the anticipated rate and expected suite of biological changes occurring within an indoor permeable setting following clinical death. Within this taphonomic context, we also address the value of describing and documenting postmortem intervals in a closed subpopulation from which some individuals are recognized for exhibiting attenuated decompositional changes. This study emphasizes the importance of addressing understudied and underrepresented regions, climates, and cultural contexts for furthering research into the factors that may contribute to the understanding of the early stages of the PMI period and improve the precision of PMI estimates more broadly.

在法医人类学和一般法医学领域,准确估计死亡时间的能力是所有法医和法医调查的重要组成部分,这些调查的重点是鉴定遗骸和死亡情况。与许多通常未知死亡日期的法医案件相比,我们从已知的死后间隔开始,通过外部检测措施(温度、皮肤弹性和肤色变化),然后通过摄影和摄像,逐步评估随着时间推移发生的分解变化。这项工作旨在提供有据可查的生物参数,为今后的研究提出假设。在气候和生态因素可能因个案而异的环境中,实地小组成员观察并记录与算法、僵硬和死亡状态相关的任何分解变化,以及皮肤颜色和组织完整性的渐进变化。我们分别在亚热带潮湿气候区和热带季风气候区(Köppen-Geiger 分类气候区)对这两例尸体进行了长达 31 天(死后间隔 [PMI] 38 天)和 19 天(死后间隔 27 天)的跟踪观察,在这些气候区观察到的延迟分解和腐败变化与临床死亡后在室内可渗透环境中发生的预期生物变化速度和预期生物变化组合不一致。在这种古乐彩网学背景下,我们还探讨了在一个封闭的亚群中描述和记录死后时间间隔的价值,在这个亚群中,有些个体被认为表现出衰减的分解变化。这项研究强调了解决研究不足和代表性不足的地区、气候和文化背景的重要性,以进一步研究可能有助于了解 PMI 早期阶段的因素,并更广泛地提高 PMI 估计的精确性。
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引用次数: 0
Machetes: Sharpening materials and methods – Producing different striation patterns on bone 弯刀:磨刀材料和方法 - 在骨头上形成不同的条纹图案
Q3 Medicine Pub Date : 2024-05-14 DOI: 10.1016/j.fsir.2024.100369
Penny McCardle

As forensic anthropologists play a larger role in post-mortem examinations, the examination and interpretation of skeletal trauma resulting from sharp, blunt, projectile, and thermal injuries have become of critical importance and may assist in medicolegal death investigations. Although there is a plethora of research on sharp force trauma on bone, at present, there is little research regarding the striations produced by large bladed weapons on bone, whether those striations are weapon-specific, or whether they are the result of sharpening materials and methods. To test whether striations are weapon type specific, this study aimed to examine if different sharpening materials and methods produced different striations on the kerf walls of cut marks on bone produced by machetes. Or, if they do produce the previously identified ‘rolling hill’ striation pattern. Four factory machetes sharpened by the same manufacturer were re-sharpened using a wet stone, a bastard file, and a coarse and medium-grained machine grinder. The bones and casts were subjected to both macro and micro analyses, and the results identified clear differences in the shape of striation ridges and troughs, indicating that striation patterns are not weapon type-specific. The study's findings have opened up a new area of research into weapon types and the analysis of associated striation patterns and may aid in the identification of weapon types and the materials used for maintenance or sharpening, potentially offering additional evidentiary evidence.

随着法医人类学家在尸检中发挥越来越大的作用,对锐器伤、钝器伤、抛射物伤和热伤造成的骨骼创伤进行检查和解释变得至关重要,并可能有助于法医死亡调查。虽然有关锐器对骨骼造成创伤的研究很多,但目前有关大型带刃武器在骨骼上产生的条纹、这些条纹是武器特有的还是磨刃材料和方法的结果等方面的研究却很少。为了检验条纹是否具有武器类型的特异性,本研究旨在考察不同的磨刀材料和方法是否会在弯刀在骨骼上切割痕迹的刀口壁上产生不同的条纹。或者,它们是否会产生之前确定的 "滚动山丘 "条纹模式。我们使用湿石、锉刀、粗粒度和中粒度机磨对同一制造商生产的四把弯刀进行了重新磨削。对骨骼和铸件进行了宏观和微观分析,结果发现条纹脊和槽的形状存在明显差异,表明条纹图案并非武器类型所特有。研究结果为武器类型和相关条纹图案的分析开辟了一个新的研究领域,可能有助于确定武器类型和用于维护或磨砺的材料,从而提供更多的证据。
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引用次数: 0
Sex determination from foramen magnum parameters in Thai cadaveric donor 根据泰国尸体供体的枕骨大孔参数确定性别
Q3 Medicine Pub Date : 2024-05-11 DOI: 10.1016/j.fsir.2024.100371
Natipong Chatthai , Napakorn Sangchay , Parkpoom Piyaman , Parichart Pattarapanitchai , Sirinart Chomean , Chollanot Kaset

The foramen magnum (FM) occupies a well-protected position, making it particularly intriguing for forensic research. The necessity for methods to estimate sex from cranial fragments becomes evident when only a partial skull is available for identification. The objective of this study was to assess sex differences in anthropometric measurements, including the length of the foramen magnum (FML), width of the foramen magnum (FMW), foramen magnum area (FMA), and circumference, with the aim of developing a binary logistic regression model in Thai cadaveric donors. Sixty adult dry skulls underwent both intraclass and interclass correlation analyses. Subsequently, 240 adult dry skulls of known sex (120 males and 120 females) were meticulously measured and analyzed to determine the optimal threshold for sex determination. Morphometric analysis of the FM was carried out using vernier calipers, and the area of the FM was precisely calculated. The FML, FMW, FMA, and circumference were all found to be significantly larger in males than in females. Furthermore, FM circumference exhibited an AUC (Area Under the Curve) value of 0.908, signifying it as an excellent parameter for sex discrimination. Lastly, binary logistic regression was executed. Significant sex differences in FM circumference were consistently observed, demonstrating an accuracy of 81.62% when validated in 120 dry skulls.

枕骨大孔(FM)的位置受到很好的保护,因此对法医研究特别有吸引力。当只有部分头骨可供鉴定时,根据头骨碎片估计性别的方法的必要性就显而易见了。本研究的目的是评估人体测量数据(包括枕骨大孔长度(FML)、枕骨大孔宽度(FMW)、枕骨大孔面积(FMA)和周长)的性别差异,以便在泰国尸体捐献者中建立二元逻辑回归模型。对 60 个成人干头骨进行了类内和类间相关性分析。随后,对已知性别的 240 个成人干头骨(120 个男性和 120 个女性)进行了细致的测量和分析,以确定确定性别的最佳阈值。使用游标卡尺对FM进行形态分析,精确计算FM的面积。结果发现,男性的 FML、FMW、FMA 和周长均明显大于女性。此外,FM 周长的 AUC(曲线下面积)值为 0.908,表明它是一个很好的性别鉴别参数。最后,进行了二元逻辑回归。在 120 个干燥头骨中验证后,发现 FM 周长始终存在显著的性别差异,准确率为 81.62%。
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引用次数: 0
Assessing the reliability and accuracy of sex estimation models utilizing sternal morphometry derived from computed tomography in the Ghanaian population 利用计算机断层扫描得出的胸骨形态测量结果,评估性别估计模型在加纳人口中的可靠性和准确性
Q3 Medicine Pub Date : 2024-05-01 DOI: 10.1016/j.fsir.2024.100368
Moses Banyeh , Abdul-Rafik Abdulai , Ernest Kofi Annan , Emmanuel Kofi Mensah , Charles Nkansah , Jeffrey Adom Nathan , Margaret Birago Twum , Paul Aghana Achumboro

Sex estimation models are specific to populations and cannot be generalized due to genetic and environmental variabilities. This retrospective cross-sectional study, conducted between January and September 2023 at the Tamale Teaching Hospital, included 119 (50.9 %) females and 115 (49.1 %) males aged 23–82 years. Measurements, including manubrium length (M), manubrium width (MW), sternal body length (B), combined manubrium and sternal body lengths (CL), corpus sterni width at first sternebrae (CSWS1), and corpus sterni width at third sternebrae (CSWS3), were obtained from Computerized Tomographic (CT) images of the sternum using DICOM Viewer, accurate to 0.1 cm. Subsequently, sternal area (SA) and sternal index (SI) were calculated. Univariable and stepwise multivariable discriminant function analysis (DFA) and logistic regression (LR) models were developed using a training sample (70 %), and cross-validation was performed on a holdout sample (30 %). Results showed that the linear measurements, excluding M, and sternal area were higher in males than females (P<0.001), while the sternal index was <50 % in males but >50 % in females (P<0.001). Univariable sex estimation accuracies, in cross-validation, ranged from 43.7 % to 92.9 % in DFA and 50.0–92.9 % in LR. For multivariable models, the accuracy ranges were 92.9–94.3 % in DFA and 91.6–93.0 % in LR. The sternal body length was the most accurate at 90.1 % in DFA and 90.2 % in LR, with lower sex bias (male-female) in LR than in DFA (-0.1 vs. 8.7). The sternum proves valuable for sex estimation, with sternal body length as the most accurate linear measurement. However, multivariable models, particularly LR, demonstrate higher accuracy compared to DFA.

性别估计模型是针对特定人群的,由于遗传和环境的变异性而无法推广。这项回顾性横断面研究于 2023 年 1 月至 9 月在塔马利教学医院进行,包括 119 名(50.9%)女性和 115 名(49.1%)男性,年龄在 23-82 岁之间。测量数据包括胸骨长度(M)、胸骨宽度(MW)、胸骨体长度(B)、胸骨和胸骨联合长度(CL)、第一节胸骨处胸骨宽度(CSWS1)和第三节胸骨处胸骨宽度(CSWS3),测量数据来自使用 DICOM Viewer 的胸骨计算机断层扫描(CT)图像,精确到 0.1 厘米。随后计算胸骨面积(SA)和胸骨指数(SI)。利用训练样本(70%)建立了单变量和逐步多变量判别函数分析(DFA)和逻辑回归(LR)模型,并对保留样本(30%)进行了交叉验证。结果显示,男性的线性测量值(不包括 M)和胸骨面积均高于女性(P<0.001),而胸骨指数男性为 50%,女性为 50%(P<0.001)。在交叉验证中,DFA 的单变量性别估计准确率为 43.7% 至 92.9%,LR 为 50.0% 至 92.9%。对于多变量模型,DFA 的准确率范围为 92.9%-94.3%,LR 为 91.6%-93.0%。胸骨体长的准确率最高,DFA 为 90.1%,LR 为 90.2%,LR 的性别偏差(男性-女性)低于 DFA(-0.1 vs. 8.7)。胸骨对性别估计很有价值,胸骨体长是最准确的线性测量值。然而,与 DFA 相比,多变量模型,尤其是 LR,显示出更高的准确性。
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引用次数: 0
Height and sex estimation using percutaneous humeral length among Ghanaians 利用经皮肱骨长度估算加纳人的身高和性别
Q3 Medicine Pub Date : 2024-04-26 DOI: 10.1016/j.fsir.2024.100367
Joshua Tetteh , Thomas Diby , Chrissie Stansie Abaidoo , Joseph Yorke , Juliet Robertson , James Nketsiah

Introduction

Humerus is a long bone that contributes significantly to the total height of an individual. It is sexually dimorphic and is a well-preserved bone postmortem. Height and sex are essential in establishing one's identity and can be estimated from long bones when standing height cannot be easily obtained. Previous studies report that sex and height are population-specific. Hence, the present study sought to establish the relationship between percutaneous humeral lengths and height and formulate models for height and sex determination for the Ghanaian population.

Materials and methods

Measurement of standing height and percutaneous humeral lengths were taken from 286 Ghanaian consented participants with no noticeable lower and upper extremities traumas or pathologies. The data was analysed using Microsoft Excel 2013 and SPSS version 23.0 edition. Correlation analysis was done to determine the correlation of the percutaneous humeral length with height, linear regression and binary logistic analyses were used to derive equations for predicting height and sex from percutaneous humeral length. An independent samples t-test was used to determine the means between males and females, left and right percutaneous humeral lengths.

Results

There was a strong positive correlation between standing height and left (r = 0.774) and right (r=0.759) humeral lengths. The left humeral length regression equation was a good height estimator in males (75.4%) and females (71.%). The left and right humeral lengths best predicted the sex for the population, with an accuracy of 78.0% and 77.8% for males and 53.8% and 54.6% for females, respectively.

Conclusion

This study has added to the limited data on the use of percutaneous humeral length as a substitute for the determination of standing height and sex in the Ghanaian population, and it is also a significant identification tool which can be employed in the field of forensic anthropometry.

导言umerus 是一种长骨,对个体的总身高有重大贡献。它具有性别二态性,是一种保存完好的死后骨骼。身高和性别对于确定一个人的身份至关重要,在无法轻易获得站立身高的情况下,可以通过长骨来估算。以往的研究报告表明,性别和身高具有人群特异性。因此,本研究试图确定肱骨经皮长度与身高之间的关系,并为加纳人口的身高和性别确定制定模型。材料和方法测量站立身高和肱骨经皮长度的对象是286名经同意的加纳人,他们没有明显的上下肢创伤或病变。数据使用 Microsoft Excel 2013 和 SPSS 23.0 版进行分析。相关性分析用于确定经皮肱骨长度与身高的相关性,线性回归和二元逻辑分析用于根据经皮肱骨长度得出预测身高和性别的方程。结果站立身高与左侧(r=0.774)和右侧(r=0.759)肱骨长度之间存在很强的正相关。左肱骨长度回归方程对男性(75.4%)和女性(71.%)的身高估计效果良好。结论:这项研究补充了关于使用经皮肱骨长度来替代测定加纳人口站立身高和性别的有限数据,它也是一种重要的鉴定工具,可用于法医人体测量领域。
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引用次数: 0
Survivability of IED components, suitcases, their contents and luggage containers in suitcase bombs 手提箱炸弹中简易爆炸装置部件、手提箱、箱内物品和行李箱的生存能力
Q3 Medicine Pub Date : 2024-04-15 DOI: 10.1016/j.fsir.2024.100366
Erwin Vermeij

One of the most striking attacks on a commercial passenger airliner was the bombing of Pan Am Flight 103 (PA103) on 21 December 1988. Shortly after departure, the Boeing 747 “Clipper Maid of the Seas” broke apart over the Scottish town of Lockerbie. All 259 passengers and crew were killed, along with 11 residents of Lockerbie. The bombing of flight PA103 is still subject to controversy. The origin of a crucial piece of evidence, a fragment of circuit board, is highly questioned. One of the reasons for this ongoing controversy is probably that circumstantial evidence, that led to the conviction of a Libyan, is not supported by other evidence. Almost nothing is published in the forensic literature regarding the survivability of components that make up an improvised explosive device (IED). To gain insight into the survivability of IED components, suitcases, their contents, and luggage containers, a series of controlled experiments were carried out in which suitcase bombs, positioned in different configurations in aluminium LD3 luggage containers, were detonated. From the results, it could be seen that the major part of the suitcases, their contents and the LD3 containers survived the explosion. Also, almost all components of the IED itself survived the explosion to a greater or lesser extent.

1988 年 12 月 21 日泛美航空公司 103 号航班(PA103)爆炸事件是对商业客机最引人注目的袭击之一。起飞后不久,这架波音 747 "Clipper Maid of the Seas "客机在苏格兰小镇洛克比上空解体。259 名乘客和机组人员全部遇难,还有 11 名洛克比居民。PA103 号航班被炸至今仍存在争议。一个关键证据--一块电路板碎片--的来源备受质疑。争议持续不断的原因之一可能是,导致一名利比亚人被定罪的间接证据没有得到其他证据的支持。法医文献中几乎没有关于简易爆炸装置(IED)部件存活性的内容。为了深入了解简易爆炸装置组件、手提箱、箱内物品和行李箱的存活能力,我们进行了一系列受控实验,将手提箱炸弹以不同配置放置在 LD3 铝制行李箱中引爆。实验结果表明,手提箱、箱内物品和 LD3 行李箱的大部分在爆炸中幸存下来。此外,简易爆炸装置本身的几乎所有部件都或多或少地在爆炸中幸存下来。
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引用次数: 0
Utilizing differential extraction thresholds to deduce the existence of spermatozoa in forensic casework samples 利用差异提取阈值推断法医案件样本中精子的存在
Q3 Medicine Pub Date : 2024-03-30 DOI: 10.1016/j.fsir.2024.100365
Evelyn Ridgley, Casey Dejournett, Karen Olson

Testing of evidence in an alleged sexual assault case not only seeks to address the question of who was involved, but also looks to answer the question of what biological source provided the DNA. It can be difficult to obtain positive serological data on challenging samples such as laundered items, low-level DNA samples, or sexual assault kit swabs obtained after a prolonged interval from the time of assault. In the absence of confirmatory serological results, an expert witness often cannot speak to the biological source of the DNA. In order to determine quantitation thresholds which could be used to deduce the presence of spermatozoa (sperm) within a sample, we evaluated the fractionation of male DNA utilizing our laboratory’s differential extraction method. Study samples included serial dilutions of semen and semen/saliva mixtures, post-coital and laundered samples as well as casework data from 1,729 samples that were processed using a differential extraction. Based on this data, it was determined that a sample which had at least 200 picograms of male DNA and at least 10% of the total male DNA in fraction 2 (F2, also known as the sperm-enriched or sperm fraction) could be reported as positive for the presence of sperm. No false positive results were obtained from the study-generated samples when using these thresholds to infer the presence of sperm. Additionally, samples that contained sperm, but were negative using traditional serological methods, could be detected. However, not all sperm-containing samples fractionated above both thresholds; therefore, serological testing may still be necessary to minimize false negative results. The thresholds developed here, proved reliable to deduce the presence of sperm in real casework samples.

对指控性侵犯案件中的证据进行检测,不仅是为了解决谁参与了性侵犯的问题,也是为了回答是什么生物来源提供了 DNA 的问题。对于具有挑战性的样本,例如洗过的物品、低水平的 DNA 样本或在与性侵犯发生时间相隔较长时间后获得的性侵犯工具箱拭子,可能很难获得阳性血清学数据。在没有确证血清学结果的情况下,专家证人往往无法说明 DNA 的生物来源。为了确定可用于推断样本中精子(精液)存在的定量阈值,我们利用实验室的差分提取法对男性 DNA 的分馏进行了评估。研究样本包括精液和精液/唾液混合物的系列稀释液、性交后样本和洗涤样本,以及使用差分提取法处理的 1,729 份样本的个案工作数据。根据这些数据确定,如果样本中至少含有 200 皮克的男性 DNA,且第 2 部分(F2,又称精子富集部分或精子部分)中的男性 DNA 至少占总 DNA 的 10%,则可报告样本中精子的存在呈阳性。使用这些阈值来推断精子是否存在时,研究产生的样本没有出现假阳性结果。此外,含有精子但使用传统血清学方法检测为阴性的样本也能被检测出来。不过,并非所有含精子样本的分馏结果都高于这两个阈值;因此,仍有必要进行血清学检测,以尽量减少假阴性结果。事实证明,在实际案例样本中推断精子存在的阈值是可靠的。
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Forensic Science International: Reports
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