C. Fraser, Atiqah Zulhisam, M. Akmal, M. Zamri-Saad, A. Salleh
Polycystic kidney disease (PKD) is an inherited disease commonly reported in cats, but rarely in big cats. It is associated with formation of cysts in the kidneys. This article describes the clinical, pathology and immunohistochemistry findings of PKD in a Siberian hybrid tiger. A 16-year-old female captive Siberian hybrid tiger showed inappetence, weight loss, polyuria, and polydipsia. Within six months, its body weight and hydration status were progressively declining. Although hematology parameters were normal, serum creatinine and urea levels were elevated. Ultrasonography of the kidneys showed the presence of cysts. The animal was euthanized and necropsied, revealing multiple cysts in both kidneys and liver. These samples were subjected to histopathology using routine stain, special histochemistry, and immunohistochemistry. The cysts were lined by various types of epithelial cells. Most renal tubular epithelium showed severe vacuolar degeneration, accompanied by bacterial colonies in the renal pelvis. Masson’s trichrome stain demonstrated presence of mesenchymal stroma at the periphery of the cysts in the kidneys and liver. Immunohistochemistry showed different staining intensity of β-catenin, E-cadherin, and vimentin in the various parts of renal tubules, suggesting the involvement of epithelial-mesenchymal transformation. The tiger was diagnosed with PKD based on clinical and pathological examination. This is the first report of PKD in a tiger, potentially useful for future study in wildlife medicine and conservation.
{"title":"Multiple renal and liver cysts in a Siberian hybrid tiger","authors":"C. Fraser, Atiqah Zulhisam, M. Akmal, M. Zamri-Saad, A. Salleh","doi":"10.12982/vis.2023.012","DOIUrl":"https://doi.org/10.12982/vis.2023.012","url":null,"abstract":"Polycystic kidney disease (PKD) is an inherited disease commonly reported in cats, but rarely in big cats. It is associated with formation of cysts in the kidneys. This article describes the clinical, pathology and immunohistochemistry findings of PKD in a Siberian hybrid tiger. A 16-year-old female captive Siberian hybrid tiger showed inappetence, weight loss, polyuria, and polydipsia. Within six months, its body weight and hydration status were progressively declining. Although hematology parameters were normal, serum creatinine and urea levels were elevated. Ultrasonography of the kidneys showed the presence of cysts. The animal was euthanized and necropsied, revealing multiple cysts in both kidneys and liver. These samples were subjected to histopathology using routine stain, special histochemistry, and immunohistochemistry. The cysts were lined by various types of epithelial cells. Most renal tubular epithelium showed severe vacuolar degeneration, accompanied by bacterial colonies in the renal pelvis. Masson’s trichrome stain demonstrated presence of mesenchymal stroma at the periphery of the cysts in the kidneys and liver. Immunohistochemistry showed different staining intensity of β-catenin, E-cadherin, and vimentin in the various parts of renal tubules, suggesting the involvement of epithelial-mesenchymal transformation. The tiger was diagnosed with PKD based on clinical and pathological examination. This is the first report of PKD in a tiger, potentially useful for future study in wildlife medicine and conservation.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41826794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sirilak Meesuwan, D. Sreta, Rachan Uppaicha, D. Ketpun
This retrospective study surveyed the histological prevalence of 180 canine neoplasms registered during 2016-2020 in the small animal hospital, the Rajamangala University of Technology Tawan-Ok, Chonburi, Thailand. Histopathologic diagnosis of each neoplasia was performed using its published diagnosis criteria. The neoplasms were categorized into epithelial, mesenchymal and round-cell neoplasms. Their parameters were related to sex, breed, and neoplastic location. The study consequence revealed that benign neoplasms occurred in the same frequency as malignant neoplasia. Some neoplasms were sex-dependent, particularly mammary adenocarcinoma in females and perianal (hepatoid) gland adenoma in males. The purebred dogs were prone to the malignant mammary gland and benign mesenchymal neoplasia; meanwhile, the crossbred dogs had more frequencies of benign epithelial, benign round cell and malignant mesenchymal neoplasms than the purebreds. The buttock and head were the primary locations of benign epithelial neoplasms. The mammary gland was common for mammary cancers, while the hindlimb was the predilection site of the malignant mesenchymal neoplasia. These results are similar to the thosedescribed in the previous studies from other regions of Thailand and the rest of the world with a few different points. Therefore, regional veterinarians, particularly in Chonburi province, can confidently use our study findings to set up their preliminary diagnoses for their neoplastic patients.
{"title":"Histopathologic prevalence of benign and malignant canine neoplasms in small animal hospital, Rajamangala University of Technology Tawan-Ok, Chonburi, Thailand: A retrospective study","authors":"Sirilak Meesuwan, D. Sreta, Rachan Uppaicha, D. Ketpun","doi":"10.12982/vis.2023.019","DOIUrl":"https://doi.org/10.12982/vis.2023.019","url":null,"abstract":"This retrospective study surveyed the histological prevalence of 180 canine neoplasms registered during 2016-2020 in the small animal hospital, the Rajamangala University of Technology Tawan-Ok, Chonburi, Thailand. Histopathologic diagnosis of each neoplasia was performed using its published diagnosis criteria. The neoplasms were categorized into epithelial, mesenchymal and round-cell neoplasms. Their parameters were related to sex, breed, and neoplastic location. The study consequence revealed that benign neoplasms occurred in the same frequency as malignant neoplasia. Some neoplasms were sex-dependent, particularly mammary adenocarcinoma in females and perianal (hepatoid) gland adenoma in males. The purebred dogs were prone to the malignant mammary gland and benign mesenchymal neoplasia; meanwhile, the crossbred dogs had more frequencies of benign epithelial, benign round cell and malignant mesenchymal neoplasms than the purebreds. The buttock and head were the primary locations of benign epithelial neoplasms. The mammary gland was common for mammary cancers, while the hindlimb was the predilection site of the malignant mesenchymal neoplasia. These results are similar to the thosedescribed in the previous studies from other regions of Thailand and the rest of the world with a few different points. Therefore, regional veterinarians, particularly in Chonburi province, can confidently use our study findings to set up their preliminary diagnoses for their neoplastic patients.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46652113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Hidayah, K. Kustantinah, C. Noviandi, A. Astuti, C. Hanim, B. Suwignyo
Studies on the identification and characterization of numerous seaweed species from tropical oceans have not been widely reported. The objective of this study was to evaluate the rumen in vitro gas production and fermentation characteristics of four tropical seaweed species. The design of treatments was a randomized complete block design with four different seaweed species (brown seaweed: Laminaria sp. and Padina australis; red seaweed: Gracilaria sp. and Eucheuma cottonii) and four replications for each treatment. For the gas production, easily degraded fraction (a), potentially degraded fraction (b), rate of gas production of b fraction (c), and total fraction degraded (a+b) parameters of brown seaweed were higher and faster than those of red seaweed. The lowest methane production at 24 hours incubation was obtained for Gracilaria sp., which reduced methane production from blank (44.38%) and standard (Pangola substrate) samples (60.63%), followed by Padina australis at 28.98 and 49.73% respectively. Padina australis resulted in the highest propionate proportion (16.03%), lowest butyrate (11.92%) and A:P ratio (4.52) (P<0.05). There were no differences in NH3 (34.17-37.31 mg/100 mL) or microbe protein concentration (9.03-10.60 mg/100 mL) among the seaweed species. It was concluded that brown seaweed (Laminaria sp. and Padina australis) were more degradable than red seaweed (Gracilaria sp. and Eucheuma cottonii) in the rumen. Padina australis is the most potential as ruminant feed because it resulted in the highest propionate proportion, the lowest butyrate and A:P ratio, and low methane production and did not disturb the NH3 and microbe protein concentrations.
{"title":"Evaluation of rumen in vitro gas production and fermentation characteristics of four tropical seaweed species","authors":"N. Hidayah, K. Kustantinah, C. Noviandi, A. Astuti, C. Hanim, B. Suwignyo","doi":"10.12982/vis.2023.018","DOIUrl":"https://doi.org/10.12982/vis.2023.018","url":null,"abstract":"Studies on the identification and characterization of numerous seaweed species from tropical oceans have not been widely reported. The objective of this study was to evaluate the rumen in vitro gas production and fermentation characteristics of four tropical seaweed species. The design of treatments was a randomized complete block design with four different seaweed species (brown seaweed: Laminaria sp. and Padina australis; red seaweed: Gracilaria sp. and Eucheuma cottonii) and four replications for each treatment. For the gas production, easily degraded fraction (a), potentially degraded fraction (b), rate of gas production of b fraction (c), and total fraction degraded (a+b) parameters of brown seaweed were higher and faster than those of red seaweed. The lowest methane production at 24 hours incubation was obtained for Gracilaria sp., which reduced methane production from blank (44.38%) and standard (Pangola substrate) samples (60.63%), followed by Padina australis at 28.98 and 49.73% respectively. Padina australis resulted in the highest propionate proportion (16.03%), lowest butyrate (11.92%) and A:P ratio (4.52) (P<0.05). There were no differences in NH3 (34.17-37.31 mg/100 mL) or microbe protein concentration (9.03-10.60 mg/100 mL) among the seaweed species. It was concluded that brown seaweed (Laminaria sp. and Padina australis) were more degradable than red seaweed (Gracilaria sp. and Eucheuma cottonii) in the rumen. Padina australis is the most potential as ruminant feed because it resulted in the highest propionate proportion, the lowest butyrate and A:P ratio, and low methane production and did not disturb the NH3 and microbe protein concentrations.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41473560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The stereotypic behaviors are common in captive bear. The objectives of study were to investigate 1) the effects of new enrichment tools on frequency of hand sucking/licking (HS) and pacing in captive sun bear; 2) the response of sun bear to each new enrichment tool. The data collection was divided into 5 parts: 1) before using enrichment 2) using puzzle feeder (PF) 3) using bamboo cradle) (BC) 4) using container (C) and 5) without using any new enrichment tool. The results showed that the frequency of HS in sun bear was not significantly affected by new enrichment tools (P>0.05). The lowest frequency of pacing was found after using new enrichment tools (P<0.001). However, it was not significantly different between before and after using new enrichment tools. The frequency of pacing using PF was lower than that of using BC and C. The highest duration of response to the new enrichment tool was in PF (P=0.002). In conclusion, using new enrichment tools could not significantly reduce the frequency of hand-sucking/licking in captive sun bear. However, it could stimuli the frequency of pacing in sun bear. The captive sun bear would be more interested in the edible enrichment tool, which could reduce the frequency of pacing compared with using other types of enrichment tools. Therefore, it is necessary to conduct a long-term experimental enrichment tool using program in the future study.
{"title":"A preliminary study of the effects of enrichment tools on stereotypic behavior in captive Sun Bear (Helarctos malayanus)","authors":"X. Huo, Khwanchanok Ongpimai, Supattra Kamwongpanao, Tanat Uttaraviset, Wirongrong Changphet, Pariyakorn Lekthong","doi":"10.12982/vis.2023.010","DOIUrl":"https://doi.org/10.12982/vis.2023.010","url":null,"abstract":"The stereotypic behaviors are common in captive bear. The objectives of study were to investigate 1) the effects of new enrichment tools on frequency of hand sucking/licking (HS) and pacing in captive sun bear; 2) the response of sun bear to each new enrichment tool. The data collection was divided into 5 parts: 1) before using enrichment 2) using puzzle feeder (PF) 3) using bamboo cradle) (BC) 4) using container (C) and 5) without using any new enrichment tool. The results showed that the frequency of HS in sun bear was not significantly affected by new enrichment tools (P>0.05). The lowest frequency of pacing was found after using new enrichment tools (P<0.001). However, it was not significantly different between before and after using new enrichment tools. The frequency of pacing using PF was lower than that of using BC and C. The highest duration of response to the new enrichment tool was in PF (P=0.002). In conclusion, using new enrichment tools could not significantly reduce the frequency of hand-sucking/licking in captive sun bear. However, it could stimuli the frequency of pacing in sun bear. The captive sun bear would be more interested in the edible enrichment tool, which could reduce the frequency of pacing compared with using other types of enrichment tools. Therefore, it is necessary to conduct a long-term experimental enrichment tool using program in the future study.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47895502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
in heat-stressed broiler chickens. One hundred and twenty broiler chicks were allocated into four groups of 30 each. Group A (feed restriction), Group B (feed restriction + L-serine), Group C (ad libitum) and Group D (ad libitum + L-serine). Feed restriction (20%) was implemented on days 7–14, and L-serine (200 mg/kg) was provided orally from days 1–14. Seven broiler chickens were slaughtered from each group at 35 days old to determine meat drip loss, cooking loss and pH; and tibia bone weight, length, diameter, weight/length index, robusticity index, strength and proximate analysis. Serum samples were harvested for the determination of calcium and phosphorus concentrations. Temperature-humidity index in the pen (28.72 - 32.90) was above the thermoneutral zone indicating heat stress. The drip loss and cooking loss were lower in FR + L-serine and AL + L-serine groups compared to the controls. The tibia bone weight, length, weight/length index and breaking force were higher (P < 0.05) in FR + L-serine and AL + L-serine groups compared to the controls. The percentage composition of ash, calcium and phosphorus was relatively high in L-serine-administered groups. In conclusion, L-serine improved meat quality and tibia bone characteristics in broiler chickens exposed to heat stress
{"title":"Effects of L-serine administration on meat quality, characteristics and mineral content of tibia bone in heat-stressed broiler","authors":"N. E. Ogbuagu, J. Ayo","doi":"10.12982/vis.2023.011","DOIUrl":"https://doi.org/10.12982/vis.2023.011","url":null,"abstract":"in heat-stressed broiler chickens. One hundred and twenty broiler chicks were allocated into four groups of 30 each. Group A (feed restriction), Group B (feed restriction + L-serine), Group C (ad libitum) and Group D (ad libitum + L-serine). Feed restriction (20%) was implemented on days 7–14, and L-serine (200 mg/kg) was provided orally from days 1–14. Seven broiler chickens were slaughtered from each group at 35 days old to determine meat drip loss, cooking loss and pH; and tibia bone weight, length, diameter, weight/length index, robusticity index, strength and proximate analysis. Serum samples were harvested for the determination of calcium and phosphorus concentrations. Temperature-humidity index in the pen (28.72 - 32.90) was above the thermoneutral zone indicating heat stress. The drip loss and cooking loss were lower in FR + L-serine and AL + L-serine groups compared to the controls. The tibia bone weight, length, weight/length index and breaking force were higher (P < 0.05) in FR + L-serine and AL + L-serine groups compared to the controls. The percentage composition of ash, calcium and phosphorus was relatively high in L-serine-administered groups. In conclusion, L-serine improved meat quality and tibia bone characteristics in broiler chickens exposed to heat stress","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48011315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dental problems in equines can cause emaciation, colic, and poor performance. Types of dental disease in equine populations have been reported worldwide in veterinary medicine; however, few detailed investigations of equine dental problems in Thailand have been published. Thus, this study aimed to determine the prevalence of equine dental problems in Thailand. Standard dental and oral cavity examinations were performed on 244 equines (232 horses and 12 donkeys) ranging in age from 2–21 years (median = 9.88) from Western, Northern, and Central parts of Thailand between January 2015 and December 2017. The dental disorders were recorded and the data was analyzed to determine the proportion as a percentage of dental disorders. Logistic regression was used to find relations between dental floatation routine, gender, age, body weight, and dental disorders. The survey showed that sharp cheek teeth (82.37%) was the most common problem, which was often associated with frontal hook (10.65%) and caudal hook (6.55%). Other disorders observed included incisor tartar (9.42%) and canine teeth tartar (9.42%). One to two years routine of dental examination and floatation has 2.54 times (p<0.01) higher chance to develop sharp teeth compared to 0.5-1 year routine. In addition, not having any routine increased this effect by 22 times(p<0.01). Sharp cheek teeth was by far the most found and significant dental problem in these equines. These findings show the importance of regular dental floating and tartar scaling. Early detection and treatment can improve equine oral health.
{"title":"A clinical survey of 244 equines with dental disorders in Thailand","authors":"P. Rungsri, Nuttapon Satumay, Phreuthi Pornprasitroj, Phuthita Rueangareerat, Supphavit Ninthisen, Supasinee Chaiya, Kannika Na-Lampang","doi":"10.12982/vis.2023.015","DOIUrl":"https://doi.org/10.12982/vis.2023.015","url":null,"abstract":"Dental problems in equines can cause emaciation, colic, and poor performance. Types of dental disease in equine populations have been reported worldwide in veterinary medicine; however, few detailed investigations of equine dental problems in Thailand have been published. Thus, this study aimed to determine the prevalence of equine dental problems in Thailand. Standard dental and oral cavity examinations were performed on 244 equines (232 horses and 12 donkeys) ranging in age from 2–21 years (median = 9.88) from Western, Northern, and Central parts of Thailand between January 2015 and December 2017. The dental disorders were recorded and the data was analyzed to determine the proportion as a percentage of dental disorders. Logistic regression was used to find relations between dental floatation routine, gender, age, body weight, and dental disorders. The survey showed that sharp cheek teeth (82.37%) was the most common problem, which was often associated with frontal hook (10.65%) and caudal hook (6.55%). Other disorders observed included incisor tartar (9.42%) and canine teeth tartar (9.42%). One to two years routine of dental examination and floatation has 2.54 times (p<0.01) higher chance to develop sharp teeth compared to 0.5-1 year routine. In addition, not having any routine increased this effect by 22 times(p<0.01). Sharp cheek teeth was by far the most found and significant dental problem in these equines. These findings show the importance of regular dental floating and tartar scaling. Early detection and treatment can improve equine oral health.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45189137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tawanhathai Apichaimongkonkun, A. Laikul, Sarocha Limratchapong, Jutamas Leklub, W. Cherdchutham
A mare aged 9 years underwent surgical correction of a small intestinal volvulus using a side-to-side, hand-sewn jejunocecal anastomosis. Nine months after the jejunocecostomy, the horse developed colic signs and ultrasonographic examination revealed gas in the large colon in the upper right flank region and several enlarged loops of the small intestine in the lower right flank area. The differential diagnosis was right dorsal displacement of the large colon or small intestinal strangulation. The exploratory celiotomy found epiploic foramen entrapment (EFE) of the jejunocecostomy anastomosis, including parts of the distal jejunum and cecum. Manual reduction of herniation of the bowel in the epiploic foramen (EF) was impossible and the horse was euthanized. The postmortem gross finding revealed the enlarged edematous at the distal end of the jejunum and the initial body portion of the cecum had passed into the EF in the right-to-left (antegrade) direction. To the authors’knowledge, this is the first report of the antegrade epiploic foramen entrapment of a jejunocecostomy bowel part and this may be one of the complications encountered during the long-term survival period of a surgical jejunocecostomy in horses.
{"title":"Entrapment of a jejunocecostomy anastomosis in the epiploic foramen in a horse","authors":"Tawanhathai Apichaimongkonkun, A. Laikul, Sarocha Limratchapong, Jutamas Leklub, W. Cherdchutham","doi":"10.12982/vis.2023.007","DOIUrl":"https://doi.org/10.12982/vis.2023.007","url":null,"abstract":"A mare aged 9 years underwent surgical correction of a small intestinal volvulus using a side-to-side, hand-sewn jejunocecal anastomosis. Nine months after the jejunocecostomy, the horse developed colic signs and ultrasonographic examination revealed gas in the large colon in the upper right flank region and several enlarged loops of the small intestine in the lower right flank area. The differential diagnosis was right dorsal displacement of the large colon or small intestinal strangulation. The exploratory celiotomy found epiploic foramen entrapment (EFE) of the jejunocecostomy anastomosis, including parts of the distal jejunum and cecum. Manual reduction of herniation of the bowel in the epiploic foramen (EF) was impossible and the horse was euthanized. The postmortem gross finding revealed the enlarged edematous at the distal end of the jejunum and the initial body portion of the cecum had passed into the EF in the right-to-left (antegrade) direction. To the authors’knowledge, this is the first report of the antegrade epiploic foramen entrapment of a jejunocecostomy bowel part and this may be one of the complications encountered during the long-term survival period of a surgical jejunocecostomy in horses.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43892442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kitiya Kongthong, Natthawut Charoenphon, N. Thaochan, P. Boonyoung, A. lida, Archig Jeamah, Supapong Imsonpang, K. Wongkamhaeng, Piyamat Kongtueng, Sinlapachai Senarat Senarat
This study aimed to increase knowledge about the structure and health assessment for evaluating histopathology as a biomarker of marine invertebrates, which must have high-quality histologic slides. Unfortunately, the accuracy of this method has been specifically modified for the marine invertebrates in Thailand; we demonstrated a modified method for permanent histological slides, including Xestospongia sp., Halobates hayanus, Amphibalanus amphitrite, and Alpheus sp. (n = 30) individual specimens for each species) from Libong island, Thailand, which was compared to the standard method. Optimization of tissue adapts and troubleshoots typical histological procedures, including sample collection, fixation with 10% Neutral Buffered Formalin at 2-4 °C for 24 hrs., decalcification, dehydration, clearing, embedding sectioning, and examining after staining under the modified procedures each time, which can be accomplished easily in six days. Compared to the standard method, this modified protocol showed the first report. It warranted high-quality slide scans, which are particularly ideal for the tissue, cell, and nuclei-specific features of marine invertebrates.
{"title":"A histological method for marine invertebrates","authors":"Kitiya Kongthong, Natthawut Charoenphon, N. Thaochan, P. Boonyoung, A. lida, Archig Jeamah, Supapong Imsonpang, K. Wongkamhaeng, Piyamat Kongtueng, Sinlapachai Senarat Senarat","doi":"10.12982/vis.2023.020","DOIUrl":"https://doi.org/10.12982/vis.2023.020","url":null,"abstract":"This study aimed to increase knowledge about the structure and health assessment for evaluating histopathology as a biomarker of marine invertebrates, which must have high-quality histologic slides. Unfortunately, the accuracy of this method has been specifically modified for the marine invertebrates in Thailand; we demonstrated a modified method for permanent histological slides, including Xestospongia sp., Halobates hayanus, Amphibalanus amphitrite, and Alpheus sp. (n = 30) individual specimens for each species) from Libong island, Thailand, which was compared to the standard method. Optimization of tissue adapts and troubleshoots typical histological procedures, including sample collection, fixation with 10% Neutral Buffered Formalin at 2-4 °C for 24 hrs., decalcification, dehydration, clearing, embedding sectioning, and examining after staining under the modified procedures each time, which can be accomplished easily in six days. Compared to the standard method, this modified protocol showed the first report. It warranted high-quality slide scans, which are particularly ideal for the tissue, cell, and nuclei-specific features of marine invertebrates.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43082532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Yamsakul, T. Yano, K. Na Lampang, Manad Khamkong, L. Srikitjakarn
Presently, the body temperature of farm animals must be monitored to prevent the occurrence or progression of any disease amongst the herds. We have employed infrared sensors (called “Inspect”) to detect the fever status of sows. Systemic architecture and data flow systems have also been designed for workers to use on small-scale pig farms. The body temperature of 100 gestating sows was determined with the use of a standard thermometer (inserted into the rectum), while our device was used on each part of the body of the sows. The valva or anus was found to be that location because of the high correlation that was observed between the two measurements (R=0.78). Moreover, regular, and systematic inspections were employed for a full year in 2019 on commercial pig farms that were home to at least 300 sows. The results indicated that the production indexes of the after period (2019) were better than those of the before period (2018), especially in terms of the health status of the animals with regard to mg/PCU. Consequently, it was determined that this system could detect abnormal signs in livestock before they could become a bigger problem.
{"title":"Infrared Temperature Sensor for Use Among Sow Herds","authors":"P. Yamsakul, T. Yano, K. Na Lampang, Manad Khamkong, L. Srikitjakarn","doi":"10.12982/vis.2023.005","DOIUrl":"https://doi.org/10.12982/vis.2023.005","url":null,"abstract":"Presently, the body temperature of farm animals must be monitored to prevent the occurrence or progression of any disease amongst the herds. We have employed infrared sensors (called “Inspect”) to detect the fever status of sows. Systemic architecture and data flow systems have also been designed for workers to use on small-scale pig farms. The body temperature of 100 gestating sows was determined with the use of a standard thermometer (inserted into the rectum), while our device was used on each part of the body of the sows. The valva or anus was found to be that location because of the high correlation that was observed between the two measurements (R=0.78). Moreover, regular, and systematic inspections were employed for a full year in 2019 on commercial pig farms that were home to at least 300 sows. The results indicated that the production indexes of the after period (2019) were better than those of the before period (2018), especially in terms of the health status of the animals with regard to mg/PCU. Consequently, it was determined that this system could detect abnormal signs in livestock before they could become a bigger problem.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47982209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen H. Tran, T. Chuc, Phi Nguyen Bang, Huu Nguyen Hung
Spirocercosis is caused by Spirocerca spp., which is a chronic disease and might cause life-threatening due to forming cancer in oesophagus in canid carnivores. There are limited studies involving spirocercosis in domestic dogs. Thus, this study aims to investigate the prevalence and analyse risk factors involved in the S. lupi infection in Mekong Delta in Vietnam. In total, 400 fecal samples from domestic dogs were collected from May 2020 to May 2021. The overall prevalence of spirocercosis in domestic dogs in the Mekong Delta was 10.50% by copromicroscope and PCR methods. PCR targeted to the housekeeping gene cytochrome c oxidase I (cox-1) was applied to identify species of Spirocerca spp. and analyse the phylogenetic tree. Outdoor dogs had 5.48 times (CI 95% = 2.45-11.690, p < 0.001) higher risks of S. lupi infection compared to indoor dogs. Besides, seasons and age showed a correlation to the increase the risk of S. lupi infection, while neither dog breeds nor gender influenced the prevalence of this species. The cytochrome c oxidase I (cox-1) gene sequence of S. lupi in the Mekong Delta showed the high homologues to the S. lupi isolates in India, Israel, and the North of Vietnam and belonged to the S. lupi genotype 2.
{"title":"The prevalence and risk factors of Spirocerca lupi in domestic dogs in the Mekong Delta of Vietnam","authors":"Nguyen H. Tran, T. Chuc, Phi Nguyen Bang, Huu Nguyen Hung","doi":"10.12982/vis.2023.002","DOIUrl":"https://doi.org/10.12982/vis.2023.002","url":null,"abstract":"Spirocercosis is caused by Spirocerca spp., which is a chronic disease and might cause life-threatening due to forming cancer in oesophagus in canid carnivores. There are limited studies involving spirocercosis in domestic dogs. Thus, this study aims to investigate the prevalence and analyse risk factors involved in the S. lupi infection in Mekong Delta in Vietnam. In total, 400 fecal samples from domestic dogs were collected from May 2020 to May 2021. The overall prevalence of spirocercosis in domestic dogs in the Mekong Delta was 10.50% by copromicroscope and PCR methods. PCR targeted to the housekeeping gene cytochrome c oxidase I (cox-1) was applied to identify species of Spirocerca spp. and analyse the phylogenetic tree. Outdoor dogs had 5.48 times (CI 95% = 2.45-11.690, p < 0.001) higher risks of S. lupi infection compared to indoor dogs. Besides, seasons and age showed a correlation to the increase the risk of S. lupi infection, while neither dog breeds nor gender influenced the prevalence of this species. The cytochrome c oxidase I (cox-1) gene sequence of S. lupi in the Mekong Delta showed the high homologues to the S. lupi isolates in India, Israel, and the North of Vietnam and belonged to the S. lupi genotype 2.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41273141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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