Anantaporn Saengthong, Areerath Akatvipat, S. Boonyayatra
This study investigates the vertebral fracture and dislocation patterns, spinal cord segment injury location, paretic grading, and the 6-month outcome in cats at a university veterinary hospital. The medical records and radiographs of cats with traumatic injuries were reviewed from October 2016 to December 2019. Eighty-nine cats were diagnosed with vertebral fracture and dislocation. The most damaged location was T3–L3 (36/89, 40.45%), followed by L4–L7 (33/89, 37.07%), S1–S3 (18/89, 20.22), C1–C5 (1/89, 1.12%), and C6–T2 (1/89, 1.12%). The patterns of vertebral damage could be classified as burst/compression (24/89, 26.96%), subluxation (19/89, 21.35%), complete luxation (16/89, 17.97%), combined fracture, and luxation (19/89, 21.35%), transverse fracture (10/69, 11.23%), and hyperextension (1/89, 1.12%). No hyperflexion-damaged pattern was detected in 89 cats. The T3–L7 lesion data was thoroughly examined (69 cats). Most of them preferred non-surgical treatment (33/69, 47.83%). Only 30 cats in the non-surgical group and 4 cats in the surgical group had tracking information. Six months after treatment, 60% of cats in the non-surgical group had better outcomes (18/30), while 66.67% of cats in the surgical group had better outcomes (4/6). Two cats in the surgical group had died of parvovirus infection. The mortality rate increased from 16.67% (6/36) at 3 months to 25% (9/36) at 6 months after treatment. All cats with paretic grade 5 had a poor prognosis. Most cats with paretic grades 1–4 receiving treatment had better clinical outcomes within 6 months and gradually improved. Follow-up should be performed for >6 months.
{"title":"Vertebral fracture and dislocation patterns, location of injuries, and 6-month clinical outcomes in cats: A retrospective study","authors":"Anantaporn Saengthong, Areerath Akatvipat, S. Boonyayatra","doi":"10.12982/vis.2022.030","DOIUrl":"https://doi.org/10.12982/vis.2022.030","url":null,"abstract":"This study investigates the vertebral fracture and dislocation patterns, spinal cord segment injury location, paretic grading, and the 6-month outcome in cats at a university veterinary hospital. The medical records and radiographs of cats with traumatic injuries were reviewed from October 2016 to December 2019. Eighty-nine cats were diagnosed with vertebral fracture and dislocation. The most damaged location was T3–L3 (36/89, 40.45%), followed by L4–L7 (33/89, 37.07%), S1–S3 (18/89, 20.22), C1–C5 (1/89, 1.12%), and C6–T2 (1/89, 1.12%). The patterns of vertebral damage could be classified as burst/compression (24/89, 26.96%), subluxation (19/89, 21.35%), complete luxation (16/89, 17.97%), combined fracture, and luxation (19/89, 21.35%), transverse fracture (10/69, 11.23%), and hyperextension (1/89, 1.12%). No hyperflexion-damaged pattern was detected in 89 cats. The T3–L7 lesion data was thoroughly examined (69 cats). Most of them preferred non-surgical treatment (33/69, 47.83%). Only 30 cats in the non-surgical group and 4 cats in the surgical group had tracking information. Six months after treatment, 60% of cats in the non-surgical group had better outcomes (18/30), while 66.67% of cats in the surgical group had better outcomes (4/6). Two cats in the surgical group had died of parvovirus infection. The mortality rate increased from 16.67% (6/36) at 3 months to 25% (9/36) at 6 months after treatment. All cats with paretic grade 5 had a poor prognosis. Most cats with paretic grades 1–4 receiving treatment had better clinical outcomes within 6 months and gradually improved. Follow-up should be performed for >6 months.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45298932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. P. Le, Huynh Anh Luu, Khanh Nguyen Huan Pham, Tan Loc Huynh, T. Chau, Thi Hong Mo Trinh, H. X. Nguyen, Trong Ngu Nguyen
This research aimed to determine the optimal media and temperature conditions for the long-term storage of bacteriophages. In this study, the viability of Salmonella phages in 50% glycerol, 10% sodium chloride-magnesium sulfate (SM) buffer, and 5% dimethyl sulfoxide (DMSO) media at room temperature, 4oC, -20oC, and -80oC for 12 months was determined. In 50% glycerol, at the end of the experiment, no significant difference was found between four temperature conditions on phage density, ranging from 6.20-6.23 log10 PFU/mL (P>0.05). Under 10% SM medium, phage preservation at room temperature provided the optimum density at 6.31 log10 PFU/mL. In addition, phages preserved in a 5% DMSO medium were of similar density values across all temperature treatments. Still, their availability after 12 month-storage (88.0-88.5%) was significantly lower (P<0.05) than that of 50% glycerol and 10% SM. Moreover, for phage lysis capacity, low temperatures (4oC, -20oC, and -80oC) were superior to room temperature used for preservation. Considering the density, lysis capacity, and practical convenience, storing phages at 4°C in a 50% Glycerol medium is recommended.
{"title":"The effect of different media and temperature conditions for Salmonella bacteriophage preservation","authors":"N. P. Le, Huynh Anh Luu, Khanh Nguyen Huan Pham, Tan Loc Huynh, T. Chau, Thi Hong Mo Trinh, H. X. Nguyen, Trong Ngu Nguyen","doi":"10.12982/vis.2022.036","DOIUrl":"https://doi.org/10.12982/vis.2022.036","url":null,"abstract":"This research aimed to determine the optimal media and temperature conditions for the long-term storage of bacteriophages. In this study, the viability of Salmonella phages in 50% glycerol, 10% sodium chloride-magnesium sulfate (SM) buffer, and 5% dimethyl sulfoxide (DMSO) media at room temperature, 4oC, -20oC, and -80oC for 12 months was determined. In 50% glycerol, at the end of the experiment, no significant difference was found between four temperature conditions on phage density, ranging from 6.20-6.23 log10 PFU/mL (P>0.05). Under 10% SM medium, phage preservation at room temperature provided the optimum density at 6.31 log10 PFU/mL. In addition, phages preserved in a 5% DMSO medium were of similar density values across all temperature treatments. Still, their availability after 12 month-storage (88.0-88.5%) was significantly lower (P<0.05) than that of 50% glycerol and 10% SM. Moreover, for phage lysis capacity, low temperatures (4oC, -20oC, and -80oC) were superior to room temperature used for preservation. Considering the density, lysis capacity, and practical convenience, storing phages at 4°C in a 50% Glycerol medium is recommended.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43069006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Setiawan, P. Chuammitri, K. Sringarm, M. Intanon, A. Sathanawongs
The development of assisted reproductive technologies (ART) in mammalian species such as in vitro embryo production (IVEP) has the potential to provide great benefits for significant population increase, improve genetic performance and advancement, and reduce transmission of venereal diseases. Correspondingly, in vitro capacitation of sperm is also paramount, related to the ability of sperm to fertilize oocytes, and was created to imitate in vivo conditions in the female reproductive tract. Amid in vitro capacitation developments, studies on how far in vitro capacitation has progressed in mimicking in vivo scenes have not been thoroughly reviewed as a comparative form. Therefore, the present study outlined the series of alterations in mammalian sperm capacitation during their journey in the female reproductive tract by exploring and juxtaposing processes under in vivo and in vitro conditions. Several essential aspects that become gaps between in vivo and in vitro were also identified and elaborated comprehensively in this systematic literature review. We noted that although in vitro capacitation procedures in certain mammalian species have made promising progress and improvements, it is still poorly successful in other species like horses. Our findings further postulated that the occurrence of cryocapacitation, the high ratio of capacitated sperm/oocyte required for successful fertilization, and the incidence of polyspermy cause capacitation under in vitro settings is less efficient and not yet fully comparable to in vivo. This work is therefore proposed several aspects that need to be bettered from in vitro milieu to make it analogous to in vivo environments in modulating sperm capacitation.
{"title":"Mammalian sperm capacitation: In vivo and in vitro juxtaposition","authors":"H. Setiawan, P. Chuammitri, K. Sringarm, M. Intanon, A. Sathanawongs","doi":"10.12982/vis.2022.026","DOIUrl":"https://doi.org/10.12982/vis.2022.026","url":null,"abstract":"The development of assisted reproductive technologies (ART) in mammalian species such as in vitro embryo production (IVEP) has the potential to provide great benefits for significant population increase, improve genetic performance and advancement, and reduce transmission of venereal diseases. Correspondingly, in vitro capacitation of sperm is also paramount, related to the ability of sperm to fertilize oocytes, and was created to imitate in vivo conditions in the female reproductive tract. Amid in vitro capacitation developments, studies on how far in vitro capacitation has progressed in mimicking in vivo scenes have not been thoroughly reviewed as a comparative form. Therefore, the present study outlined the series of alterations in mammalian sperm capacitation during their journey in the female reproductive tract by exploring and juxtaposing processes under in vivo and in vitro conditions. Several essential aspects that become gaps between in vivo and in vitro were also identified and elaborated comprehensively in this systematic literature review. We noted that although in vitro capacitation procedures in certain mammalian species have made promising progress and improvements, it is still poorly successful in other species like horses. Our findings further postulated that the occurrence of cryocapacitation, the high ratio of capacitated sperm/oocyte required for successful fertilization, and the incidence of polyspermy cause capacitation under in vitro settings is less efficient and not yet fully comparable to in vivo. This work is therefore proposed several aspects that need to be bettered from in vitro milieu to make it analogous to in vivo environments in modulating sperm capacitation.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41299594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Dejkong, Suchat Wattanachai, P. Phuektes, Suphannika Putthachalee, S. Angkititrakul
Salmonella is one of the major causes of foodborne diseases in humans. These bacteria can colonize within the gastrointestinal tracts of both humans and animals, and there have been reports of incidences of Salmonella in horses. This study aimed to investigate the prevalence, serotypes, and antimicrobial resistance of Salmonella isolates from racehorses and horsemen, as well as to explore the possible transmission between horses and humans. Fecal samples from racehorses (247 samples) and horsemen (33 samples) were collected from horse farms located in 3 provinces of upper Northeastern Thailand between March and August 2019. Salmonella was isolated and identified. Broth microdilution was used to determine the minimal inhibitory concentrations (MICs) of the antimicrobial agents for antimicrobial. Salmonella isolates were detected in 4.86% (12/247) of racehorses and 3.03% (1/33) of horsemen. The most commonly found serotypes in the isolates obtained from the racehorses were Abony (25%) and Iganda (16.67%). Only the Tumodi II serotype was found in one horseman. Salmonella isolates collected from the racehorses had been the most resistant to streptomycin (66.67%), while the isolate from a horsemen had been resistant to ampicillin, streptomycin, oxytetracycline, and to tetracycline. Although Salmonella transmission between racehorses and horsemen was not found to be obviously present in this study, the appropriate use of antimicrobials and hygienic procedures are still necessary in order to prevent antimicrobial resistance and the transmission of drug-resistant Salmonella between horses and humans.
{"title":"Epidemiology and Antimicrobial Resistance of Salmonella Isolated from Racehorses and Horsemen in Northeastern Thailand","authors":"R. Dejkong, Suchat Wattanachai, P. Phuektes, Suphannika Putthachalee, S. Angkititrakul","doi":"10.12982/vis.2022.037","DOIUrl":"https://doi.org/10.12982/vis.2022.037","url":null,"abstract":"Salmonella is one of the major causes of foodborne diseases in humans. These bacteria can colonize within the gastrointestinal tracts of both humans and animals, and there have been reports of incidences of Salmonella in horses. This study aimed to investigate the prevalence, serotypes, and antimicrobial resistance of Salmonella isolates from racehorses and horsemen, as well as to explore the possible transmission between horses and humans. Fecal samples from racehorses (247 samples) and horsemen (33 samples) were collected from horse farms located in 3 provinces of upper Northeastern Thailand between March and August 2019. Salmonella was isolated and identified. Broth microdilution was used to determine the minimal inhibitory concentrations (MICs) of the antimicrobial agents for antimicrobial. Salmonella isolates were detected in 4.86% (12/247) of racehorses and 3.03% (1/33) of horsemen. The most commonly found serotypes in the isolates obtained from the racehorses were Abony (25%) and Iganda (16.67%). Only the Tumodi II serotype was found in one horseman. Salmonella isolates collected from the racehorses had been the most resistant to streptomycin (66.67%), while the isolate from a horsemen had been resistant to ampicillin, streptomycin, oxytetracycline, and to tetracycline. Although Salmonella transmission between racehorses and horsemen was not found to be obviously present in this study, the appropriate use of antimicrobials and hygienic procedures are still necessary in order to prevent antimicrobial resistance and the transmission of drug-resistant Salmonella between horses and humans.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49438947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nattinee Kittiwan, P. Tadee, Phacharaporn Tadee, Tunyamai Buawiratlert, Thanaporn Eiamsam-ang, Orawan Boonma, S. Rojanasthien, B. Pascoe, P. Patchanee
Streptococcus suis (S. suis) belongs to the critical streptococcal swine pathogens affecting financial losses in pig production globally and being concerned as a zoonotic bacterial that causes a severe invasive disease emerging in pigs and humans. Serotype 2 is considered the major serotype and is the most pathogenic S. suis obtained from human cases, and the healthy pigs are known as the major reservoir of S. suis. In this cross-sectional study, S. suis prevalence was conducted in live pigs from 111 farms across Chiang Mai, Thailand. S. suis carriage rate and serotypes were determined from the bacteriological and multiplex PCR method from tonsil swab samples. We found that 18.2% (138/760) of tonsil swab samples and 54.1% (60/111) of pig farms were positive to S. suis, and only one (0.72%) from 138 isolates was identified as serotype 9. Meanwhile, all the remains were identified as non-serotype ½/1/2/7/9/14 strains. In addition, there was an independent relation between age-ranged, farm types, and production systems with S. suis-positive rates at the farm level. The results indicate that both intensive and smallholder production systems can generally be the source of S. suis carriage. Therefore, implementing good husbandry practices and S. suis-infection predisposing factors limiting that appropriate for each farm type is essential to minimize the opportunities of S. suis outbreak in humans.
{"title":"Identification of Streptococcus suis carriage in healthy pigs in Chiang Mai, Thailand","authors":"Nattinee Kittiwan, P. Tadee, Phacharaporn Tadee, Tunyamai Buawiratlert, Thanaporn Eiamsam-ang, Orawan Boonma, S. Rojanasthien, B. Pascoe, P. Patchanee","doi":"10.12982/vis.2022.027","DOIUrl":"https://doi.org/10.12982/vis.2022.027","url":null,"abstract":"Streptococcus suis (S. suis) belongs to the critical streptococcal swine pathogens affecting financial losses in pig production globally and being concerned as a zoonotic bacterial that causes a severe invasive disease emerging in pigs and humans. Serotype 2 is considered the major serotype and is the most pathogenic S. suis obtained from human cases, and the healthy pigs are known as the major reservoir of S. suis. In this cross-sectional study, S. suis prevalence was conducted in live pigs from 111 farms across Chiang Mai, Thailand. S. suis carriage rate and serotypes were determined from the bacteriological and multiplex PCR method from tonsil swab samples. We found that 18.2% (138/760) of tonsil swab samples and 54.1% (60/111) of pig farms were positive to S. suis, and only one (0.72%) from 138 isolates was identified as serotype 9. Meanwhile, all the remains were identified as non-serotype ½/1/2/7/9/14 strains. In addition, there was an independent relation between age-ranged, farm types, and production systems with S. suis-positive rates at the farm level. The results indicate that both intensive and smallholder production systems can generally be the source of S. suis carriage. Therefore, implementing good husbandry practices and S. suis-infection predisposing factors limiting that appropriate for each farm type is essential to minimize the opportunities of S. suis outbreak in humans.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45597878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nutthakarn Suwankitwat, Wachareeporn Starrat, Preeyanuch Sagumpung, J. Ratthanophart
Porcine respiratory and reproductive syndrome virus (PRRSV) causes significant economic losses in the swine industry worldwide. Nucleocapsid protein (NP) encoded by ORF7 is the most abundant and high immunogenicity. However, there were only few studies on recombinant NP expression with antigenicity and immunogenicity from PRRSV in Thailand. The aim of this study was to express rNP from PRRSV found in Thailand and evaluate its antigenicity and immunogenicity. ORF7 gene was amplified by RT-PCR. The PCR products were cloned into pGEM®-T Easy vector, and subcloned into pET-24a (+) expression vector and transformed into E. coli. The rNP properties were proved by SDS-PAGE and Western blot. The antigenicity of rNP was evaluated by indirect ELISA comparing to a commercial test kit. The immunogenicity of rNP was proved by rabbit immunization. Then, the antiserum was tested with tissues from PRRSV-infected swine by immunohistochemistry (IHC). In addition, amino acid sequence of the rNP was analyzed and compared to PRRSV reference strains. The PCR products of a whole ORF7 gene were 388 bp. The rNP size was 18 kDa. The indirect ELISA assay showed high antigenicity of the rNP with 100% sensitivity and 93.33% specificity at 0.25 O.D. cutoff value. The rNP could induce specific antibody production in the rabbit, which reacted specifically to PRRSV in tissues by IHC. The amino acid sequence of the rNP had similarities to US, HP-PRRSV and EU PRRSV at 95.93-99.19%, 96.75%, and 57.03-61.47%, respectively. Our rNP can be used for PRRSV diagnosis and test kit development.
{"title":"Expression of recombinant porcine reproductive and respiratory syndrome virus nucleocapsid protein with antigenicity and immunogenicity study","authors":"Nutthakarn Suwankitwat, Wachareeporn Starrat, Preeyanuch Sagumpung, J. Ratthanophart","doi":"10.12982/vis.2022.029","DOIUrl":"https://doi.org/10.12982/vis.2022.029","url":null,"abstract":"Porcine respiratory and reproductive syndrome virus (PRRSV) causes significant economic losses in the swine industry worldwide. Nucleocapsid protein (NP) encoded by ORF7 is the most abundant and high immunogenicity. However, there were only few studies on recombinant NP expression with antigenicity and immunogenicity from PRRSV in Thailand. The aim of this study was to express rNP from PRRSV found in Thailand and evaluate its antigenicity and immunogenicity. ORF7 gene was amplified by RT-PCR. The PCR products were cloned into pGEM®-T Easy vector, and subcloned into pET-24a (+) expression vector and transformed into E. coli. The rNP properties were proved by SDS-PAGE and Western blot. The antigenicity of rNP was evaluated by indirect ELISA comparing to a commercial test kit. The immunogenicity of rNP was proved by rabbit immunization. Then, the antiserum was tested with tissues from PRRSV-infected swine by immunohistochemistry (IHC). In addition, amino acid sequence of the rNP was analyzed and compared to PRRSV reference strains. The PCR products of a whole ORF7 gene were 388 bp. The rNP size was 18 kDa. The indirect ELISA assay showed high antigenicity of the rNP with 100% sensitivity and 93.33% specificity at 0.25 O.D. cutoff value. The rNP could induce specific antibody production in the rabbit, which reacted specifically to PRRSV in tissues by IHC. The amino acid sequence of the rNP had similarities to US, HP-PRRSV and EU PRRSV at 95.93-99.19%, 96.75%, and 57.03-61.47%, respectively. Our rNP can be used for PRRSV diagnosis and test kit development.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42532703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During COVID-19 outbreak, alcohol-based hand sanitizer (ABHS) has been widely used for hand cleaning and removing pathogens including human pathogens and zoonosis pathogens. High concentration of alcohol induces dehydrated skin in the users. Therefore, the objective of this study was to investigate moisturizing property and antimicrobial activity of alcohol-based hand sanitizer formulations using coconut oil as a moisturizing agent against pathogens including Escherichia coli and Staphylococcus aureus. The properties including antimicrobial activity, stability of the ABHS, and satisfaction levels of the coconut oil with two existing formulations by WHO and the Ministry of Public Health of Thailand (MOPH) were determined. The formulation containing the coconut oil demonstrated antibacterial activity against both E. coli and S. aureus with the minimal inhibitory concentration (MIC) of 8.75% V/V as same as MOPH. However, the WHO-modified formulation has the highest antimicrobial activity with the MIC value of 2.19% V/V. The stability result of 3 ABHSs showed that the preserved had the same efficacy compared to that of the fresh formulations. Hand washing with coconut formulation produced an average score of 7.19±1.71 on color, 7.06±1.56 on appearance, 5.82±2.10 on odor, 6.77±1.68 on moisture, 6.88±1.42 on overall acceptance out of 9. However, the sensory analysis of these three formulations results showed no significant difference in all parameters. The data suggest that moisturizing agents tested in our study do not affect the efficacy of ethanol. The coconut oil formulation is another good option for people searching for an effective hand sanitizer for germs protection and moisturizing.
{"title":"Evaluation of moisturizing property and antimicrobial activity of alcohol-based hand sanitizer formulations using coconut oil as a moisturizing agent against Staphylococcus aureus and Escherichia coli","authors":"Saruda Kuraeiad, Pathomporn Prueksatrakun, Yada Chuajeen, Nateelak Kooltheat, Orawan Sookbampen, W. Mitsuwan, Pattamaporn Kwankaew","doi":"10.12982/vis.2022.031","DOIUrl":"https://doi.org/10.12982/vis.2022.031","url":null,"abstract":"During COVID-19 outbreak, alcohol-based hand sanitizer (ABHS) has been widely used for hand cleaning and removing pathogens including human pathogens and zoonosis pathogens. High concentration of alcohol induces dehydrated skin in the users. Therefore, the objective of this study was to investigate moisturizing property and antimicrobial activity of alcohol-based hand sanitizer formulations using coconut oil as a moisturizing agent against pathogens including Escherichia coli and Staphylococcus aureus. The properties including antimicrobial activity, stability of the ABHS, and satisfaction levels of the coconut oil with two existing formulations by WHO and the Ministry of Public Health of Thailand (MOPH) were determined. The formulation containing the coconut oil demonstrated antibacterial activity against both E. coli and S. aureus with the minimal inhibitory concentration (MIC) of 8.75% V/V as same as MOPH. However, the WHO-modified formulation has the highest antimicrobial activity with the MIC value of 2.19% V/V. The stability result of 3 ABHSs showed that the preserved had the same efficacy compared to that of the fresh formulations. Hand washing with coconut formulation produced an average score of 7.19±1.71 on color, 7.06±1.56 on appearance, 5.82±2.10 on odor, 6.77±1.68 on moisture, 6.88±1.42 on overall acceptance out of 9. However, the sensory analysis of these three formulations results showed no significant difference in all parameters. The data suggest that moisturizing agents tested in our study do not affect the efficacy of ethanol. The coconut oil formulation is another good option for people searching for an effective hand sanitizer for germs protection and moisturizing.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47586276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kiratitana-olan, W. Chaisowwong, K. Thongkorn, K. Kreausukon
Rabies is a challenging human and animal health concern that requires a multisectoral and interdisciplinary strategy for prevention and control. The aim of this study was to investigate One Health perspectives on long-term rabies prevention in disease-free subdistricts of Chiang Mai, Thailand. A qualitative study using semi-structured interviews was carried out in three rabies-free communities with diverse demographics, and the results were analyzed using thematic analysis. The findings were gathered from 36 interviews with equal proportions of urban, suburban, and rural populations. Although none of the participants mentioned the term “One Health”, the majority of them comprehended that rather than a one-dimensional approach, a multi-dimensional approach to rabies prevention would be effective. The main theme from the interview analysis was multisectoral collaboration. The role of the local government in collaboration, the involvement of the medical and veterinary branches, and intersectoral action with community engagement were all sub-themes. Community leaders and village health volunteers collaborated with local authorities to connect community members. The use of wired broadcasting as a method of public communication was critical to increasing rabies knowledge and awareness. Human and animal health agencies provided support for rabies vaccine and medical equipment, as well as staff skills and practice training. Community participation in rabies activities contributed to preventing, detecting, and responding to the disease. The One Health concept must be developed and implemented in practice at all levels of all key sectors, particularly among the general population in rabies-endemic areas throughout the country.
{"title":"One Health perspectives on sustainable rabies prevention in Thailand: a qualitative interview study","authors":"K. Kiratitana-olan, W. Chaisowwong, K. Thongkorn, K. Kreausukon","doi":"10.12982/vis.2022.033","DOIUrl":"https://doi.org/10.12982/vis.2022.033","url":null,"abstract":"Rabies is a challenging human and animal health concern that requires a multisectoral and interdisciplinary strategy for prevention and control. The aim of this study was to investigate One Health perspectives on long-term rabies prevention in disease-free subdistricts of Chiang Mai, Thailand. A qualitative study using semi-structured interviews was carried out in three rabies-free communities with diverse demographics, and the results were analyzed using thematic analysis. The findings were gathered from 36 interviews with equal proportions of urban, suburban, and rural populations. Although none of the participants mentioned the term “One Health”, the majority of them comprehended that rather than a one-dimensional approach, a multi-dimensional approach to rabies prevention would be effective. The main theme from the interview analysis was multisectoral collaboration. The role of the local government in collaboration, the involvement of the medical and veterinary branches, and intersectoral action with community engagement were all sub-themes. Community leaders and village health volunteers collaborated with local authorities to connect community members. The use of wired broadcasting as a method of public communication was critical to increasing rabies knowledge and awareness. Human and animal health agencies provided support for rabies vaccine and medical equipment, as well as staff skills and practice training. Community participation in rabies activities contributed to preventing, detecting, and responding to the disease. The One Health concept must be developed and implemented in practice at all levels of all key sectors, particularly among the general population in rabies-endemic areas throughout the country.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47654040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pirun Chutipongvivate, K. Na Lampang, V. Punyapornwithaya, K. Thongkorn
A sterilization program is implied to control the cat population and set up as national rabies control activities. In Thailand, a few studies have been conducted on postoperative complications and undesired incidents after massive sterilization. This study determined the incidence of short-term sterilization complications in cats with or without postoperative antibiotic administration and the risk of short-term surgical complications. The investigators conducted a clinical trial study. Female cats (n=492) who participated in Chiang Mai municipal birth control activity were assessed and recorded those complications between with and without postoperative antibiotics. The risk factors were analyzed using risk ratios (RR) with a 95% confidence level. Twenty-five cats (5.08%) showed short-term complications in the study. The incidence of short-term complications was not significantly different between cats with postoperative antibiotics (4.92%) and without postoperative antibiotics (5.24%). Cats with external parasites had 2.35 times a short-term complication (RR=1.05-5.27;95%Cl). Duration in captive area, this factor showed that prolong of a cat kept in cage or indoor area play role as a protective factor which less than 2-day in a cage or indoor as the baseline, the risk ratios were 0.38 in 5-day and 0.18 in 7-day, respectively. In conclusion, cat sterilization without post-operative antibiotic administration could be performed under ordinary operation with an aseptic concept.
{"title":"Incidence of short-term complications after sterilization between pre-operative antibiotic versus pre- and post-operative antibiotic administration in female cats","authors":"Pirun Chutipongvivate, K. Na Lampang, V. Punyapornwithaya, K. Thongkorn","doi":"10.12982/vis.2022.028","DOIUrl":"https://doi.org/10.12982/vis.2022.028","url":null,"abstract":"A sterilization program is implied to control the cat population and set up as national rabies control activities. In Thailand, a few studies have been conducted on postoperative complications and undesired incidents after massive sterilization. This study determined the incidence of short-term sterilization complications in cats with or without postoperative antibiotic administration and the risk of short-term surgical complications. The investigators conducted a clinical trial study. Female cats (n=492) who participated in Chiang Mai municipal birth control activity were assessed and recorded those complications between with and without postoperative antibiotics. The risk factors were analyzed using risk ratios (RR) with a 95% confidence level. Twenty-five cats (5.08%) showed short-term complications in the study. The incidence of short-term complications was not significantly different between cats with postoperative antibiotics (4.92%) and without postoperative antibiotics (5.24%). Cats with external parasites had 2.35 times a short-term complication (RR=1.05-5.27;95%Cl). Duration in captive area, this factor showed that prolong of a cat kept in cage or indoor area play role as a protective factor which less than 2-day in a cage or indoor as the baseline, the risk ratios were 0.38 in 5-day and 0.18 in 7-day, respectively. In conclusion, cat sterilization without post-operative antibiotic administration could be performed under ordinary operation with an aseptic concept.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48307414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-26DOI: 10.21203/rs.3.rs-1250626/v1
L. Tunsisa, Kefyalew Berihun
Poultry is one of the most important subsectors of livestock, supplying a cheap source of good quality animal protein in the form of meat and eggs. This study was conducted to assess egg fertility, hatchability and egg quality parameters of indigenous chickens in the Hulla, Aleta Wondo and Dale districts, representing highland, midland and lowland agroecologies, respectively. Six kebeles (two kebeles from each district) were purposively selected based on a lower distribution of exotic chickens. A total of 750 eggs of indigenous chicken were collected from three different agro-ecologies, 450 eggs were used for fertility and hatchability evaluation, and 300 eggs were used for internal and external quality analysis. The collected data were analyzed using SPSS version 20 and SAS version 9.0 packages. The results indicated that agroecology has no significant effect on the fertility and hatchability of indigenous chicken eggs. The values of egg weight, egg length, shell weight, yolk weight, and yolk diameter and albumen weight of eggs collected from midland were higher than those of eggs from highland and lowland areas. However, the highest values for albumen height, yolk height and Haugh unit were observed on eggs collected from highland agroecology. In conclusion, agroecology has no effect on the fertility and hatchability of indigenous chicken eggs. However, agroecology significantly affects the internal and external egg quality of indigenous chicken eggs. Therefore, the storage condition and duration of storage need consideration to maintain the external quality of eggs.
家禽是畜牧业中最重要的分部门之一，以肉和蛋的形式提供廉价的优质动物蛋白来源。本研究对分别代表高原、中部和低地农业生态的Hulla、Aleta Wondo和Dale地区土鸡的产蛋率、孵化率和鸡蛋品质参数进行了评估。根据外来鸡的较低分布，有目的地选择了6只kebeles(每个区2只kebeles)。从3个不同的农业生态区共采集了750个土鸡蛋，其中450个用于育性和孵化性评价，300个用于内部和外部质量分析。采用SPSS version 20和SAS version 9.0软件包对收集的数据进行分析。结果表明，农业生态对土鸡蛋的育性和孵化率无显著影响。中部地区采蛋的蛋重、蛋长、壳重、蛋黄重、蛋黄直径和蛋白重均高于高原和低地地区采蛋的蛋重。而蛋白高度、蛋黄高度和哈氏单位均以高原农业生态采集的鸡蛋最高。综上所述，农业生态学对土鸡蛋的育性和孵化率没有影响。然而，农业生态学对土鸡鸡蛋的内在品质和外在品质影响显著。因此，为了保持鸡蛋的外在品质，需要考虑贮藏条件和贮藏时间。
{"title":"Evaluation of fertility, hatchability and egg quality of indigenous chicken at different agro-ecologies of Sidama Region, Ethiopia","authors":"L. Tunsisa, Kefyalew Berihun","doi":"10.21203/rs.3.rs-1250626/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-1250626/v1","url":null,"abstract":"\u0000 Poultry is one of the most important subsectors of livestock, supplying a cheap source of good quality animal protein in the form of meat and eggs. This study was conducted to assess egg fertility, hatchability and egg quality parameters of indigenous chickens in the Hulla, Aleta Wondo and Dale districts, representing highland, midland and lowland agroecologies, respectively. Six kebeles (two kebeles from each district) were purposively selected based on a lower distribution of exotic chickens. A total of 750 eggs of indigenous chicken were collected from three different agro-ecologies, 450 eggs were used for fertility and hatchability evaluation, and 300 eggs were used for internal and external quality analysis. The collected data were analyzed using SPSS version 20 and SAS version 9.0 packages. The results indicated that agroecology has no significant effect on the fertility and hatchability of indigenous chicken eggs. The values of egg weight, egg length, shell weight, yolk weight, and yolk diameter and albumen weight of eggs collected from midland were higher than those of eggs from highland and lowland areas. However, the highest values for albumen height, yolk height and Haugh unit were observed on eggs collected from highland agroecology. In conclusion, agroecology has no effect on the fertility and hatchability of indigenous chicken eggs. However, agroecology significantly affects the internal and external egg quality of indigenous chicken eggs. Therefore, the storage condition and duration of storage need consideration to maintain the external quality of eggs.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42301007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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