Pub Date : 2023-10-01DOI: 10.29267/mxjb.2023.8.4.68
Isui Guadalupe Velázquez Sánchez, Edgar Tovar Juárez, Yaneth Estrada Santos, Pedro Martín Negrete Moreno, Virginia Aurora Herrera Valencia, Santy Peraza Echeverria, Jalsen Iván Teco Bravo
Microalgae remove nitrogen and phosphorus from water for their growth and biomass production, wich has several biotechnological applications. This study evaluated the growth, NO3−, NO2−, NH4+, urea, PO43− and total nitrogen removal capacity, as well as the biomass production and the CO2 fixation of two new microalgae isolates, Chlorella sp. and Quadrigula sp., from the coast of Chiapas, México. Chlorella sp. had a higher specific growth rate (0.74/day) and a shorter doubling time (0.92 days) than Quadrigula sp. Removal per unit volume (mg/L) of NO3−, NH4+ and total nitrogen by Chlorella sp. was higher (26.88%, 10.58% and 16.63%, respectively) than removal by Quadrigula sp. On the other hand, daily removal (mg/L/d) of NO3−; NH4+; NO2−; urea and total nitrogen by Chlorella sp. was also higher (88%, 33%, 89%,15% and 50%, respectively) than daily removal by Quadrigula sp. The biomass productivity (mg/L/d) and the CO2 fixation rate (mg/L/d) were similar among both microalgae. The results of this study suggest that Chlorella sp. and Quadrigula sp. have potential for nutrient removal, biomass production and CO2 fixation.
{"title":"Nitrogen and phosphorus removal coupled to CO2 fixation by two green microalgae, Chlorella sp. and Quadrigula sp., native to the coast of Chiapas, Mexico","authors":"Isui Guadalupe Velázquez Sánchez, Edgar Tovar Juárez, Yaneth Estrada Santos, Pedro Martín Negrete Moreno, Virginia Aurora Herrera Valencia, Santy Peraza Echeverria, Jalsen Iván Teco Bravo","doi":"10.29267/mxjb.2023.8.4.68","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.4.68","url":null,"abstract":"Microalgae remove nitrogen and phosphorus from water for their growth and biomass production, wich has several biotechnological applications. This study evaluated the growth, NO3−, NO2−, NH4+, urea, PO43− and total nitrogen removal capacity, as well as the biomass production and the CO2 fixation of two new microalgae isolates, Chlorella sp. and Quadrigula sp., from the coast of Chiapas, México. Chlorella sp. had a higher specific growth rate (0.74/day) and a shorter doubling time (0.92 days) than Quadrigula sp. Removal per unit volume (mg/L) of NO3−, NH4+ and total nitrogen by Chlorella sp. was higher (26.88%, 10.58% and 16.63%, respectively) than removal by Quadrigula sp. On the other hand, daily removal (mg/L/d) of NO3−; NH4+; NO2−; urea and total nitrogen by Chlorella sp. was also higher (88%, 33%, 89%,15% and 50%, respectively) than daily removal by Quadrigula sp. The biomass productivity (mg/L/d) and the CO2 fixation rate (mg/L/d) were similar among both microalgae. The results of this study suggest that Chlorella sp. and Quadrigula sp. have potential for nutrient removal, biomass production and CO2 fixation.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135407320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.29267/mxjb.2023.8.4.46
Víctor Eric López y López, Shirlley Elizabeth Martínez Tolibia, Adrián Díaz Pacheco, Jabel Dinorín Téllez Girón, Pável Sierra Martínez
The biological control agent Bacillus thuringiensis (Bt) coordinates metabolic and gene regulation functions to enter to the differentiation process for the synthesis of spores and Cry toxin proteins. The master regulator for sporulation initiation is Spo0A, while AbrB regulator coordinate cellular functions during vegetative and transition growth. To understand how differentiation is modified during fed-batch cultures (FBC) of Bt under variable nutrient conditions, we conducted fermentations with two different nutrient concentration and feeding times using an industrial medium. Since sporulation efficiency has been lower in FBCs than in batch cultures, AbrB was monitored by Western blot during these cultures. Kinetic parameters were used to stablish timing of cell differentiation process, and fermentations were monitored on-line using impedance spectroscopy. AbrB was accumulated during the entire fed-batch culture, when a medium with the highest concentration of carbon and nitrogen sources (FBC1) were fed. Under these conditions, sporulation efficiency and cry1Ac expression were negatively affected, compared to the wild-type strain. Conversely, when the feeding concentration was only half of FBC1, AbrB accumulates in minor proportion, and sporulation efficiency and cry1Ac expression increased (FBC2). These results suggest that variable nutrient conditions can cause an imbalance in the accumulation of transition state regulators such as AbrB during FBCs, which can be detrimental to produce spores and Cry proteins, that are the main Bt products for biological control of agricultural pests.
{"title":"Influence of nutrient feeding variations on AbrB accumulation, sporulation and cry1Ac expression during fed-batch cultures of Bacillus thuringiensis","authors":"Víctor Eric López y López, Shirlley Elizabeth Martínez Tolibia, Adrián Díaz Pacheco, Jabel Dinorín Téllez Girón, Pável Sierra Martínez","doi":"10.29267/mxjb.2023.8.4.46","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.4.46","url":null,"abstract":"The biological control agent Bacillus thuringiensis (Bt) coordinates metabolic and gene regulation functions to enter to the differentiation process for the synthesis of spores and Cry toxin proteins. The master regulator for sporulation initiation is Spo0A, while AbrB regulator coordinate cellular functions during vegetative and transition growth. To understand how differentiation is modified during fed-batch cultures (FBC) of Bt under variable nutrient conditions, we conducted fermentations with two different nutrient concentration and feeding times using an industrial medium. Since sporulation efficiency has been lower in FBCs than in batch cultures, AbrB was monitored by Western blot during these cultures. Kinetic parameters were used to stablish timing of cell differentiation process, and fermentations were monitored on-line using impedance spectroscopy. AbrB was accumulated during the entire fed-batch culture, when a medium with the highest concentration of carbon and nitrogen sources (FBC1) were fed. Under these conditions, sporulation efficiency and cry1Ac expression were negatively affected, compared to the wild-type strain. Conversely, when the feeding concentration was only half of FBC1, AbrB accumulates in minor proportion, and sporulation efficiency and cry1Ac expression increased (FBC2). These results suggest that variable nutrient conditions can cause an imbalance in the accumulation of transition state regulators such as AbrB during FBCs, which can be detrimental to produce spores and Cry proteins, that are the main Bt products for biological control of agricultural pests.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135406713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.29267/mxjb.2023.8.4.1
Karen V. Enríquez-López, Jorge R. Robledo-Ortíz, Orfil González-Reynoso, Berenice Clifton-García, Yolanda González-García
The production of biomass and biopolyesters (polyhydroxyalkanoates) by the marine bacterium Saccharophagus degradans from: glucose, starch, glucosamine, and xylose were studied. The effect of yeast extract, trace elements, vitamins, and initial concentration of each carbon source on biomass production was evaluated. The addition of yeast extract, together with trace elements, increased the biomass production by 5-fold, obtaining the maximum values when using an initial substrate concentration of 20 g/L for: starch, 8.61; glucosamine, 7.06; and xylose, 4.47 (g dry cell weight/ L). From glucose at 40 g/L the maximum biomass production was 9.07 g/L. Next, the biopolymer production under nutrient-limiting conditions (by N, P, S and Mg) was investigated, as well as the ability of S. degradans to produce copolymers from precursors. The biopolyester produced in all cases was poly(3-hydroxybutyrate) (PHB). Different accumulation percentages (g PHB/100 g biomass) were achieved depending on the carbon source used: glucose, 27.72; starch, 18.11; glucosamine 3.33; there was not biopolymer accumulated from xylose. Finally, the PHB produced from each carbohydrate was characterized, presenting molecular weights between 53 and 58 KDa, and fusion temperatures between 164 and 174°C.
{"title":"Effect of culture medium composition on the growth and biopolyesters production by the marine bacterium Saccharophagus degradans","authors":"Karen V. Enríquez-López, Jorge R. Robledo-Ortíz, Orfil González-Reynoso, Berenice Clifton-García, Yolanda González-García","doi":"10.29267/mxjb.2023.8.4.1","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.4.1","url":null,"abstract":"The production of biomass and biopolyesters (polyhydroxyalkanoates) by the marine bacterium Saccharophagus degradans from: glucose, starch, glucosamine, and xylose were studied. The effect of yeast extract, trace elements, vitamins, and initial concentration of each carbon source on biomass production was evaluated. The addition of yeast extract, together with trace elements, increased the biomass production by 5-fold, obtaining the maximum values when using an initial substrate concentration of 20 g/L for: starch, 8.61; glucosamine, 7.06; and xylose, 4.47 (g dry cell weight/ L). From glucose at 40 g/L the maximum biomass production was 9.07 g/L. Next, the biopolymer production under nutrient-limiting conditions (by N, P, S and Mg) was investigated, as well as the ability of S. degradans to produce copolymers from precursors. The biopolyester produced in all cases was poly(3-hydroxybutyrate) (PHB). Different accumulation percentages (g PHB/100 g biomass) were achieved depending on the carbon source used: glucose, 27.72; starch, 18.11; glucosamine 3.33; there was not biopolymer accumulated from xylose. Finally, the PHB produced from each carbohydrate was characterized, presenting molecular weights between 53 and 58 KDa, and fusion temperatures between 164 and 174°C.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135407633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.29267/mxjb.2023.8.4.24
José Luis Espinoza-Acosta, Ramsés Ramón González-Estrada, Dora Edith Valencia-Rivera, Karla Guadalupe Martínez-Robinson, Ana Irene Ledesma-Osuna, Francisco J. Blancas-Benitez, Beatriz Montaño-Leyva
Biopolymeric films offer an ecological alternative to obtain containers or coatings for food. These offer a barrier against moisture and are biocompatible. Mixtures of different polymers make it possible to obtain materials with unique physicochemical properties. The objective of this study was to assess the effect of different soluble fraction of organosolv lignin on the antifungal and physicochemical properties of starch films. The lignin was extracted using a mixture of organic solvents, fractionated in alcohol, and the alcohol-soluble lignin fraction was incorporated into starch films at concentrations of 1, 3, 5, and 10 %. The resulting films underwent analysis through Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electronic Microscopy (SEM). Additionally, thickness, color, the mechanical properties, and the inhibition capacity against P. italicum fungus of the films were also evaluated. The FTIR and SEM results revealed significant chemical interactions between starch and alcohol-soluble lignin fraction confirming a correct integration of both polymers. Moreover, the incorporation of alcohol-soluble lignin fraction had a plasticizing effect on the films, substantially enhancing their flexibility. Regarding antifungal activity, the films with 5 % alcohol-soluble lignin fraction exhibited the highest percentage of inhibition of the P. italicum fungus (91.7 ± 0.56 %), while the lowest inhibition was obtained in the films containing 10 % (64.0 ± 0.32 %). The starch/ alcohol-soluble lignin fraction films demonstrated effective antifungal in vitro properties, providing a viable alternative to control the development microorganism that affect the shelf life of foods.
{"title":"Antifungal and physicochemical properties of starch-lignin based-films","authors":"José Luis Espinoza-Acosta, Ramsés Ramón González-Estrada, Dora Edith Valencia-Rivera, Karla Guadalupe Martínez-Robinson, Ana Irene Ledesma-Osuna, Francisco J. Blancas-Benitez, Beatriz Montaño-Leyva","doi":"10.29267/mxjb.2023.8.4.24","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.4.24","url":null,"abstract":"Biopolymeric films offer an ecological alternative to obtain containers or coatings for food. These offer a barrier against moisture and are biocompatible. Mixtures of different polymers make it possible to obtain materials with unique physicochemical properties. The objective of this study was to assess the effect of different soluble fraction of organosolv lignin on the antifungal and physicochemical properties of starch films. The lignin was extracted using a mixture of organic solvents, fractionated in alcohol, and the alcohol-soluble lignin fraction was incorporated into starch films at concentrations of 1, 3, 5, and 10 %. The resulting films underwent analysis through Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electronic Microscopy (SEM). Additionally, thickness, color, the mechanical properties, and the inhibition capacity against P. italicum fungus of the films were also evaluated. The FTIR and SEM results revealed significant chemical interactions between starch and alcohol-soluble lignin fraction confirming a correct integration of both polymers. Moreover, the incorporation of alcohol-soluble lignin fraction had a plasticizing effect on the films, substantially enhancing their flexibility. Regarding antifungal activity, the films with 5 % alcohol-soluble lignin fraction exhibited the highest percentage of inhibition of the P. italicum fungus (91.7 ± 0.56 %), while the lowest inhibition was obtained in the films containing 10 % (64.0 ± 0.32 %). The starch/ alcohol-soluble lignin fraction films demonstrated effective antifungal in vitro properties, providing a viable alternative to control the development microorganism that affect the shelf life of foods.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135407023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.29267/mxjb.2023.8.4.90
Christian Jesús Mora-Pérez, Roberto Olivares-Hernández, Ernesto Favela-Torres, Gustavo Viniegra Gonzalez
A statistical approach based on fractional and complete factorial designs to increase the citric acid yield was used. Polyurethane foam (PUF) was used as inert support for solid-state fermentation (SSF) due to its high-water retention capacity (up to 60-fold of its weight), resulting in the recovery of concentrated citric acid by squeezing it. The maximum yields for citric acid (0.62 Cmol/Cmol glucose) and polyols (0.11 Cmol/Cmol glucose) are obtained by growing Aspergillus brasiliensis in a well-defined culture medium (at 30°C for 120 h). Citric acid, polyols, and glucose consumption are negatively affected by a high glucose concentration (250 g/L). In contrast, enrichment of the culture medium with yeast extract only enhances citric acid production yield (0.54 Cmol/Cmol glucose). This study demonstrates that the citric acid production yield is negatively affected by phosphate (> 4.54 g/L) and magnesium (> 0.26 g/L). The PUF for SSF is an inexpensive and useful support for the commercial production of citric acid. The utilization of low-cost inert supports through SSF can contribute to achieving industrially viable and sustainable citric acid production. As far as we know, this work reports the highest citric acid production yield using SSF on an inert support such as PUF soaked with a defined medium.
采用基于分数和完全析因设计的统计方法来提高柠檬酸收率。聚氨酯泡沫(PUF)由于其高保水能力(高达其重量的60倍)而被用作固态发酵(SSF)的惰性载体，通过挤压可以回收浓缩的柠檬酸。柠檬酸(0.62 Cmol/Cmol葡萄糖)和多元醇(0.11 Cmol/Cmol葡萄糖)的最大产量是通过在定义明确的培养基中(在30℃下培养120小时)培养巴西曲霉获得的。高葡萄糖浓度(250 g/L)会对柠檬酸、多元醇和葡萄糖消耗产生负面影响。相比之下，用酵母提取物富集培养基只能提高柠檬酸产率(0.54 Cmol/Cmol葡萄糖)。研究表明，磷酸对柠檬酸产率有负面影响。4.54 g/L)和镁(>0.26 g / L)。对柠檬酸的商业化生产来说，PUF是一种廉价而有用的支持。通过SSF利用低成本惰性载体可以实现工业上可行和可持续的柠檬酸生产。据我们所知，这项工作报告了在惰性载体(如PUF)上使用SSF的最高柠檬酸产率。
{"title":"High citric acid production in solid-state fermentation by Aspergillus brasiliensis on polyurethane foam","authors":"Christian Jesús Mora-Pérez, Roberto Olivares-Hernández, Ernesto Favela-Torres, Gustavo Viniegra Gonzalez","doi":"10.29267/mxjb.2023.8.4.90","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.4.90","url":null,"abstract":"A statistical approach based on fractional and complete factorial designs to increase the citric acid yield was used. Polyurethane foam (PUF) was used as inert support for solid-state fermentation (SSF) due to its high-water retention capacity (up to 60-fold of its weight), resulting in the recovery of concentrated citric acid by squeezing it. The maximum yields for citric acid (0.62 Cmol/Cmol glucose) and polyols (0.11 Cmol/Cmol glucose) are obtained by growing Aspergillus brasiliensis in a well-defined culture medium (at 30°C for 120 h). Citric acid, polyols, and glucose consumption are negatively affected by a high glucose concentration (250 g/L). In contrast, enrichment of the culture medium with yeast extract only enhances citric acid production yield (0.54 Cmol/Cmol glucose). This study demonstrates that the citric acid production yield is negatively affected by phosphate (> 4.54 g/L) and magnesium (> 0.26 g/L). The PUF for SSF is an inexpensive and useful support for the commercial production of citric acid. The utilization of low-cost inert supports through SSF can contribute to achieving industrially viable and sustainable citric acid production. As far as we know, this work reports the highest citric acid production yield using SSF on an inert support such as PUF soaked with a defined medium.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135407492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.29267/mxjb.2023.8.3.1
Xunaxi Juquila Moreno-López, José Luis Villarruel-Ordaz, J. Valenzuela-Soto, L. D. Maldonado-Bonilla
Fungi of the Trichoderma genus have been widely studied for their mycoparasitic characteristics that allow the generation of biological control products. In addition, some of them promote plant growth. Currently, there are over 300 species divided into 8 clades or lineages. Despite such diversity, few species have been reported in Mexico, and there is not representativeness of every clade. Here, we present the identification of the 2IG2102 strain of T. brevicompactum based on analysis of 3 phylogenetic markers, as well as its characterization as antagonist towards 2 Fusarium fungi, and it also boosts tomato and cucumber seed germination. Trichothecene biosynthesis might be a mechanism required to inhibit phytopathogens growth and in parallel affect the post-germinative growth of tomato. This is the first report of a Trichoderma belonging to the clade 6 isolated in Mexico, that displays capacity to antagonize fungi and it might produce trichothecenes for biological control.
{"title":"A Trichoderma brevicompactum strain isolated in Mexico inhibits the growth of phytopathogenic fungi","authors":"Xunaxi Juquila Moreno-López, José Luis Villarruel-Ordaz, J. Valenzuela-Soto, L. D. Maldonado-Bonilla","doi":"10.29267/mxjb.2023.8.3.1","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.3.1","url":null,"abstract":"Fungi of the Trichoderma genus have been widely studied for their mycoparasitic characteristics that allow the generation of biological control products. In addition, some of them promote plant growth. Currently, there are over 300 species divided into 8 clades or lineages. Despite such diversity, few species have been reported in Mexico, and there is not representativeness of every clade. Here, we present the identification of the 2IG2102 strain of T. brevicompactum based on analysis of 3 phylogenetic markers, as well as its characterization as antagonist towards 2 Fusarium fungi, and it also boosts tomato and cucumber seed germination. Trichothecene biosynthesis might be a mechanism required to inhibit phytopathogens growth and in parallel affect the post-germinative growth of tomato. This is the first report of a Trichoderma belonging to the clade 6 isolated in Mexico, that displays capacity to antagonize fungi and it might produce trichothecenes for biological control.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44717181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.29267/mxjb.2023.8.3.25
G. V. Cerrillo-Rojas, R. Soria-Guerra, A. E. Ochoa-Alfaro, Hans Christian Correa-Aguado, Daniela A. Díaz-García, C. Garcidueñas-Piña, J. F. Morales-Domínguez
Soil salinity severely affects plants and specially to crop plants. Halophytes are resistant to salinity stress since they have developed several gene mechanisms of resistance, such as the enzymatic Na+ compartmentalization into vacuoles by vacuolar Pyrophosphatase (VPase) and the up-expression of transcription factors such as Dehydration-sensitive Element-Binding proteins (DREB). Halophytes are a potential source of salt-resistance genes and are considered as a study model for this type of stress. In this study, thehalophyte Suaeda edulis was used for the identification, characterization, in silico analysis and expression of the DREB and VPase genes. Deduced amino acid sequences of SeDREB and SeVP showed >80% similarity with their homologous and contain conserved domains and motifs characteristic of these proteins. Phylogeny showed that SeDREB is in the subgroup A2 that is expressed in salinity, while SeVP is in the group of the K-dependent vacuolar proteins. In silico coexpresion analysis showed the interaction with several proteins related with salinity and drought. The expression of genes was higher in roots and leaves in wild plants than in in vitro plants. The soil inhabited by S. edulis has a pH 9.2, an EC=4.2 dS m-1, as well as a translocation factor for sodium of 3.4, which indicates a higher adsorption of this metal by the plant.
{"title":"Characterization, in silico analysis and expression of DREB2 and VPase from Suaeda edulis","authors":"G. V. Cerrillo-Rojas, R. Soria-Guerra, A. E. Ochoa-Alfaro, Hans Christian Correa-Aguado, Daniela A. Díaz-García, C. Garcidueñas-Piña, J. F. Morales-Domínguez","doi":"10.29267/mxjb.2023.8.3.25","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.3.25","url":null,"abstract":"Soil salinity severely affects plants and specially to crop plants. Halophytes are resistant to salinity stress since they have developed several gene mechanisms of resistance, such as the enzymatic Na+ compartmentalization into vacuoles by vacuolar Pyrophosphatase (VPase) and the up-expression of transcription factors such as Dehydration-sensitive Element-Binding proteins (DREB). Halophytes are a potential source of salt-resistance genes and are considered as a study model for this type of stress. In this study, thehalophyte Suaeda edulis was used for the identification, characterization, in silico analysis and expression of the DREB and VPase genes. Deduced amino acid sequences of SeDREB and SeVP showed >80% similarity with their homologous and contain conserved domains and motifs characteristic of these proteins. Phylogeny showed that SeDREB is in the subgroup A2 that is expressed in salinity, while SeVP is in the group of the K-dependent vacuolar proteins. In silico coexpresion analysis showed the interaction with several proteins related with salinity and drought. The expression of genes was higher in roots and leaves in wild plants than in in vitro plants. The soil inhabited by S. edulis has a pH 9.2, an EC=4.2 dS m-1, as well as a translocation factor for sodium of 3.4, which indicates a higher adsorption of this metal by the plant.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43802245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.29267/mxjb.2023.8.3.49
Diego Salas-Valdivia, Laura Ahtziri Díaz-Godínez, J. J. Castañeda-Nava, J. Rodríguez-Domínguez
The pineapple (Ananas comosus L. Merr) is an economically important bromeliad plant due to the organoleptic characteristics of its fruit, being the MD2 variety one of the most cultivated. Pineapple culture is limited by its inefficient sucker production. Micropropagation represents a way of producing pineapple plantlets. The aim of this study was to obtain a micropropagation protocol for A. comosus MD2 variety using the axillary bud proliferation technique. Axillary buds from suckers were put into MS media where 17 treatments were tested: benzylaminopurine (BA) in combination with indolebutyric acid (IBA) (0, 0.5, 1 mg/L) or indoleacetic acid (IAA) (0, 0.5, 1 mg/L); as well as BA (2 mg/L) with naphthaleneacetic acid (NAA) (5 mg/L); and kinetin (KIN) (2 mg/L). The presence or absence of silver nanoparticles (34 mg/L) was tested as well. The percentage of sprouting, number of shoots, and length were measured. Percentage of sprouting was higher (73.3%) when kinetin and silver were present. Highest shoot number (2.5±0.49) was observed on media containing BA 3 mg/L and AIA 0.5 mg/L. Also, organogenesis was observed on media with BA and ANA. Finally, acclimatization had a 100% success rate.
{"title":"Micropropagation of pineapple (Ananas comosus L. Merr) MD2 variety through axillary bud proliferation","authors":"Diego Salas-Valdivia, Laura Ahtziri Díaz-Godínez, J. J. Castañeda-Nava, J. Rodríguez-Domínguez","doi":"10.29267/mxjb.2023.8.3.49","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.3.49","url":null,"abstract":"The pineapple (Ananas comosus L. Merr) is an economically important bromeliad plant due to the organoleptic characteristics of its fruit, being the MD2 variety one of the most cultivated. Pineapple culture is limited by its inefficient sucker production. Micropropagation represents a way of producing pineapple plantlets. The aim of this study was to obtain a micropropagation protocol for A. comosus MD2 variety using the axillary bud proliferation technique. Axillary buds from suckers were put into MS media where 17 treatments were tested: benzylaminopurine (BA) in combination with indolebutyric acid (IBA) (0, 0.5, 1 mg/L) or indoleacetic acid (IAA) (0, 0.5, 1 mg/L); as well as BA (2 mg/L) with naphthaleneacetic acid (NAA) (5 mg/L); and kinetin (KIN) (2 mg/L). The presence or absence of silver nanoparticles (34 mg/L) was tested as well. The percentage of sprouting, number of shoots, and length were measured. Percentage of sprouting was higher (73.3%) when kinetin and silver were present. Highest shoot number (2.5±0.49) was observed on media containing BA 3 mg/L and AIA 0.5 mg/L. Also, organogenesis was observed on media with BA and ANA. Finally, acclimatization had a 100% success rate.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43815332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.29267/mxjb.2023.8.2.17
Jorge de Jesús Ramos-Sánchez, Escarlett González-Morales, J. Ramírez-Vázquez, Froylán M. Espinoza-Escalante
Hyaluronic acid is a biopolymer present in a wide variety of biological structures and it is implied in multiple biochemical processes, this is why it has been suggested its artificial use to treat several illnesses and, also in cosmetology. Nowadays, recombinant bacteria seem to be the key for the biotechnological production of this molecule since non-pathogenic strains can be used and, in some cases, it could be expected to increase the production yields. Given that culture conditions have an impact on the generation of any microbial metabolite, in this work, we looked for the best culture conditions in the bioreactor. Then, we present the bioengineering analysis of hyaluronic acid production by two genetically modified strains of E. coli. We tested two plasmids and three physicochemical parameters in flasks. In this work, we demonstrated that the media composition, aeration, and the relation between agitation and volume, but the extra plasmid, impact the production of the biopolymer. Besides, it was determined the modified E. coli with genes PmHAS and kfiD is viable to produce up to 105 mg/L of hyaluronic acid in a culture media with a carbohydrate source, such as glycerol, and keeping an aeration ratio of 1.5 vvm. This last was corroborated using the carbazole method with modifications in the purification procedure of the produced acid.
{"title":"Growth culture conditions and recovery method impact the yield of hyaluronic acid produced by genetically modified Escherichia coli strains","authors":"Jorge de Jesús Ramos-Sánchez, Escarlett González-Morales, J. Ramírez-Vázquez, Froylán M. Espinoza-Escalante","doi":"10.29267/mxjb.2023.8.2.17","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.2.17","url":null,"abstract":"Hyaluronic acid is a biopolymer present in a wide variety of biological structures and it is implied in multiple biochemical processes, this is why it has been suggested its artificial use to treat several illnesses and, also in cosmetology. Nowadays, recombinant bacteria seem to be the key for the biotechnological production of this molecule since non-pathogenic strains can be used and, in some cases, it could be expected to increase the production yields. Given that culture conditions have an impact on the generation of any microbial metabolite, in this work, we looked for the best culture conditions in the bioreactor. Then, we present the bioengineering analysis of hyaluronic acid production by two genetically modified strains of E. coli. We tested two plasmids and three physicochemical parameters in flasks. In this work, we demonstrated that the media composition, aeration, and the relation between agitation and volume, but the extra plasmid, impact the production of the biopolymer. Besides, it was determined the modified E. coli with genes PmHAS and kfiD is viable to produce up to 105 mg/L of hyaluronic acid in a culture media with a carbohydrate source, such as glycerol, and keeping an aeration ratio of 1.5 vvm. This last was corroborated using the carbazole method with modifications in the purification procedure of the produced acid.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48470228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.29267/mxjb.2023.8.2.1
Verónica-Alhelí Ochoa-Jiménez, R. Balois-Morales, G. G. López-Guzmán, José Orlando Jiménez-Zurit, L. F. Guzmán, Guillermo Berumen-Varela
Rapid softening is one of the main problems of soursop (Annona muricata L.) fruits. The physicochemical parameters and gene expression response of cell-wall-related genes in soursop fruits were evaluated. The temperature of 15 ± 2 °C delayed the physicochemical composition of soursop fruits by three days and significant down-regulated the expression of cell-wall related genes. Pectin methylesterase (PME1), pectate lyase (PL22), polygalacturonase (PG), Endoglucanase (EG), and expansins (EXP13 and EXP7) genes showed a down-regulation of their expression during ripening at 28 ± 2 °C and 15 ± 2 °C. The bioinformatic analysis demonstrated two conserved domains associated with degradation of cell wall. Higher gene level of the EXP7 gene was detected on Day 6 of storage at 28 ± 2 °C, showing a 3.5-fold increase in the gene expression compared to Day 0. Furthermore, PME1, PL22, PG, EG, and EXP13 expression was down-regulated by the temperature of 15 ± 2 °C (p < 0.05). Gene expression was highly correlated in the fruits stored at 28 ± 2 °C. On the other hand, a high correlation between the EXP7, PME1, and PL22 genes was recorded on the fruits stored at 15 ± 2 °C. The multivariate analysis demonstrated that acidity is an important variable during ripening and the two EXP genes, and EG are highly correlated with acidity and negatively correlated with firmness. In conclusion, the findings of this research suggest that these genes are down-regulated by the combination of days of storage and temperature.
{"title":"Changes in quality and gene transcript levels of soursop (Annona muricata L.) fruits during ripening","authors":"Verónica-Alhelí Ochoa-Jiménez, R. Balois-Morales, G. G. López-Guzmán, José Orlando Jiménez-Zurit, L. F. Guzmán, Guillermo Berumen-Varela","doi":"10.29267/mxjb.2023.8.2.1","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.2.1","url":null,"abstract":"Rapid softening is one of the main problems of soursop (Annona muricata L.) fruits. The physicochemical parameters and gene expression response of cell-wall-related genes in soursop fruits were evaluated. The temperature of 15 ± 2 °C delayed the physicochemical composition of soursop fruits by three days and significant down-regulated the expression of cell-wall related genes. Pectin methylesterase (PME1), pectate lyase (PL22), polygalacturonase (PG), Endoglucanase (EG), and expansins (EXP13 and EXP7) genes showed a down-regulation of their expression during ripening at 28 ± 2 °C and 15 ± 2 °C. The bioinformatic analysis demonstrated two conserved domains associated with degradation of cell wall. Higher gene level of the EXP7 gene was detected on Day 6 of storage at 28 ± 2 °C, showing a 3.5-fold increase in the gene expression compared to Day 0. Furthermore, PME1, PL22, PG, EG, and EXP13 expression was down-regulated by the temperature of 15 ± 2 °C (p < 0.05). Gene expression was highly correlated in the fruits stored at 28 ± 2 °C. On the other hand, a high correlation between the EXP7, PME1, and PL22 genes was recorded on the fruits stored at 15 ± 2 °C. The multivariate analysis demonstrated that acidity is an important variable during ripening and the two EXP genes, and EG are highly correlated with acidity and negatively correlated with firmness. In conclusion, the findings of this research suggest that these genes are down-regulated by the combination of days of storage and temperature.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42706172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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