Pub Date : 2018-07-01DOI: 10.29267/MXJB.2018.3.3.77
P. Vera-Hernández, Marco Antonio Ortega-Ramírez, M. M. Núñez, M. Ruíz-Rivas, F. F. Rosas-Cárdenas
Plants have developed physiological and molecular mechanisms to support and adapt to adverse environments. One response to abiotic stress is the accumulation of free proline (PRO). PRO can induce the expression of many genes, which have the proline-responsive element (PRE) in their promoters, nevertheless due to the complexity of interactions between stress factors and various molecular, biochemical and physiological phenomena it is still unclear whether a more efficient PRO accumulation can be considered a biomarker of tolerance in plants. In the present work, we evaluated the accumulation of PRO in two genotypes of sorghum with contrasting tolerance to cold stress. To explore the cause behind the accumulation of proline under cold stress conditions, we identified the Transcription Factors Binding Sites (TFBS) present in the promoter regions in the genes involved in the biosynthesis and degradation of proline in sorghum and other important crops, finding that the untranslated 3 'region P5CS gene contains different TFBS. We found TFBS that could allow the activation of genes involved in proline biosynthesis through the ornithine pathway under cold stress conditions, suggesting that ornithine route can be activated under cold stress conditions
{"title":"Proline as a probable biomarker of cold stress tolerance in Sorghum (Sorghum bicolor)","authors":"P. Vera-Hernández, Marco Antonio Ortega-Ramírez, M. M. Núñez, M. Ruíz-Rivas, F. F. Rosas-Cárdenas","doi":"10.29267/MXJB.2018.3.3.77","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.3.77","url":null,"abstract":"Plants have developed physiological and molecular mechanisms to support and adapt to adverse environments. One response to abiotic stress is the accumulation of free proline (PRO). PRO can induce the expression of many genes, which have the proline-responsive element (PRE) in their promoters, nevertheless due to the complexity of interactions between stress factors and various molecular, biochemical and physiological phenomena it is still unclear whether a more efficient PRO accumulation can be considered a biomarker of tolerance in plants. In the present work, we evaluated the accumulation of PRO in two genotypes of sorghum with contrasting tolerance to cold stress. To explore the cause behind the accumulation of proline under cold stress conditions, we identified the Transcription Factors Binding Sites (TFBS) present in the promoter regions in the genes involved in the biosynthesis and degradation of proline in sorghum and other important crops, finding that the untranslated 3 'region P5CS gene contains different TFBS. We found TFBS that could allow the activation of genes involved in proline biosynthesis through the ornithine pathway under cold stress conditions, suggesting that ornithine route can be activated under cold stress conditions","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49207498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.29267/MXJB.2018.3.3.1
Diana G. Alamilla-Martínez, N. Rojas-Avelizapa, I. Dominguez-Lopez, J. Barceinas-Sánchez, M. Gómez-Ramírez
Recently the demand in the development of eco-friendly nanoparticles as alternative to chemical and physical methods has been increasing so the aim of this study was to evaluate the effect of silver nitrate concentration and extracellular filtrate (EF) produced by a filamentous fungus isolated from a spent catalyst and coded e identified as Penicillium purpurogenum CATMC-AH-1 on Silver nanoparticles (AgNPs) production. The filamentous fungus was growth in two culture media named Sucrose and Czapeck media to produce biomass and its was put in contact with water to get two different extracellular filtrates called EFS (extracellular filtrate sucrose) and EFC (extracellular filtrate Czapeck), the EF has the molecules involved to synthesis and stabilization of AgNPs. Three concentrations of AgNO3 1, 1.5 and 2 mM and both extracellular filtrates were used to produce AgNPs. The AgNPs produced were monitored by UV-visible absorption spectra from 200 to 800 nm while their morphology and size were identified by Transmission Electron Microscopy (TEM) and software analysis SPIP 2.6.0. Results showed that both extracellular filtrates had the ability to produce AgNPs with the three concentrations of AgNO3 used. TEM analysis showed AgNPs with spherical morphology in all systems. The AgNPs synthesized in EFS with the three AgNO3 concentrations showed average sizes of 8.9, 8.4 and 6.7 nm respectively. From EFC, the average sizes of AgNPs were of 14.9, 11.5 and 10.1 nm respectively. In summary, in EFS smallest sizes and diameter dispersion of AgNPs were obtained, comparing to EFC and the spherical shape was similar in both filtrates. The AgNO3 concentration had a positive effect when the EFC filtrate was used since a direct correlation was observed between the concentrations of silver nitrate and increase the absorption band around 420 nm as result of surface plasmon resonance of AgNPs produced. The AgNPs biosynthesized from both EF (EFS and EFC) could be used as antimicrobial agent by their small size. Parameter as silver nitrate concentrations and culture media are important because could be affect the size and concentrations of AgNPs biosynthesized.
{"title":"Effect of culture media and silver nitrate concentration on nanoparticle biosynthesis by a filamentous fungus","authors":"Diana G. Alamilla-Martínez, N. Rojas-Avelizapa, I. Dominguez-Lopez, J. Barceinas-Sánchez, M. Gómez-Ramírez","doi":"10.29267/MXJB.2018.3.3.1","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.3.1","url":null,"abstract":"Recently the demand in the development of eco-friendly nanoparticles as alternative to chemical and physical methods has been increasing so the aim of this study was to evaluate the effect of silver nitrate concentration and extracellular filtrate (EF) produced by a filamentous fungus isolated from a spent catalyst and coded e identified as Penicillium purpurogenum CATMC-AH-1 on Silver nanoparticles (AgNPs) production. The filamentous fungus was growth in two culture media named Sucrose and Czapeck media to produce biomass and its was put in contact with water to get two different extracellular filtrates called EFS (extracellular filtrate sucrose) and EFC (extracellular filtrate Czapeck), the EF has the molecules involved to synthesis and stabilization of AgNPs. Three concentrations of AgNO3 1, 1.5 and 2 mM and both extracellular filtrates were used to produce AgNPs. The AgNPs produced were monitored by UV-visible absorption spectra from 200 to 800 nm while their morphology and size were identified by Transmission Electron Microscopy (TEM) and software analysis SPIP 2.6.0. Results showed that both extracellular filtrates had the ability to produce AgNPs with the three concentrations of AgNO3 used. TEM analysis showed AgNPs with spherical morphology in all systems. The AgNPs synthesized in EFS with the three AgNO3 concentrations showed average sizes of\u00008.9, 8.4 and 6.7 nm respectively. From EFC, the average sizes of AgNPs were of 14.9, 11.5 and 10.1 nm respectively. In summary, in EFS smallest sizes and diameter dispersion of AgNPs were obtained, comparing to EFC and the spherical shape was similar in both filtrates. The AgNO3 concentration had a positive effect when the EFC filtrate was used since a direct correlation was observed between the concentrations of silver nitrate and increase the absorption band around 420 nm as result of surface plasmon resonance of AgNPs produced. The AgNPs biosynthesized from both EF (EFS and EFC) could be used as antimicrobial agent by their small size. Parameter as silver nitrate concentrations and culture media are important because could be affect the size and concentrations of AgNPs biosynthesized.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45531125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.29267/MXJB.2018.3.3.26
Daniel López-Sosa, María de Jesús García-Gómez, Oscar Núñez-Gaona
La producción de hongos entomopatógenos es un área de estudio de gran importancia representando una alternativa ante el uso de insecticidas de origen químico. Durante el mecanismo de infección los hongos entomopatógenos liberan lipasas, proteasas y quitinasas responsables de la penetración de la cutícula de los insectos. Se ha demostrado que en fermentación líquida estas enzimas son inducibles, pero los productos obtenidos por este bioproceso no tienen alta efectividad para su uso a nivel agrícola; en cambio, los obtenidos por fermentación sólida presentan una ventaja con respecto a su viabilidad, debido a la formación de conidios aéreos. El objetivo de este trabajo fue analizar cualitativamente la producción de enzimas de Beauveria bassiana en fermentación sólida utilizando mezclas de arroz y cutículas de T. molitor (100:0, 95:5, 90:10, 85:15 %). En las pruebas semicualitativas realizadas se observó que el uso de inductores en fermentación sólida no tuvo efecto con respecto a la represión total de las enzimas producidas. En cuanto a la virulencia de los conidios, el mejor tratamiento se obtuvo con una relación sustrato: inductor 90:10para producir conidios con mayor virulencia en los bioensayos.
{"title":"Análisis cualitativo de la producción de enzimas de Beauveria bassiana en fermentación sólida utilizando un inductor","authors":"Daniel López-Sosa, María de Jesús García-Gómez, Oscar Núñez-Gaona","doi":"10.29267/MXJB.2018.3.3.26","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.3.26","url":null,"abstract":"La producción de hongos entomopatógenos es un área de estudio de gran importancia representando una alternativa ante el uso de insecticidas de origen químico. Durante el mecanismo de infección los hongos entomopatógenos liberan lipasas, proteasas y quitinasas responsables de la penetración de la cutícula de los insectos. Se ha demostrado que en fermentación líquida estas enzimas son inducibles, pero los productos obtenidos por este bioproceso no tienen alta efectividad para su uso a nivel agrícola; en cambio, los obtenidos por fermentación sólida presentan una ventaja con respecto a su viabilidad, debido a la formación de conidios aéreos. El objetivo de este trabajo fue analizar cualitativamente la producción de enzimas de Beauveria bassiana en fermentación sólida utilizando mezclas de arroz y cutículas de T. molitor (100:0, 95:5, 90:10, 85:15 %). En las pruebas semicualitativas realizadas se observó que el uso de inductores en fermentación sólida no tuvo efecto con respecto a la represión total de las enzimas producidas. En cuanto a la virulencia de los conidios, el mejor tratamiento se obtuvo con una relación sustrato: inductor 90:10para producir conidios con mayor virulencia en los bioensayos.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44885922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.29267/mxjb.2018.3.3.36
Francisco David Moreno-Valencia, M. Plascencia-Espinosa, J. Rojas
Se realizo el aislamiento y seleccion segun su mecanismo de accion de bacterias endofiticas y rizosfericas promotoras de crecimiento vegetal de poblaciones silvestres de Pinus patula y Pinus montezumae, grupos arboreos de coniferas mexicanas utilizados con fines de reforestacion por su crecimiento acelerado y alto rendimiento. Ochenta y siete cepas bacterianas fueron aisladas de plantulas de pino, purificadas y agrupadas por criterios morfologicos. Se evaluaron y seleccionaron cepas que producen fitoreguladores de crecimiento, solubilizacion de fosfato, determinacion de la cantidad de fosforo solubilizado y produccion de quitinasa. Treinta y siete cepas mostraron al menos una de las actividades evaluadas, con niveles de produccion de fitorreguadores en parametros que van de 54.4 a 139 µg/ml, halos alrededor de las colonias de 3.2 a 3.8 cm solubilizando fosfato en cantidades que varian de 0.1 a 2.4 mg/L y crecimiento completo con zonas claras alrededor de las colonias con halos de 1.2 a 2 cm para la produccion de quitinasa. Los resultados de este trabajo representan el primer paso para desarrollar un sistema de aceleracion de crecimiento para plantas de uso forestal
本研究以墨西哥针叶树(Pinus patula)和montezumae松(Pinus montezumae)野生种群为研究对象，根据其促进植物生长的内生细菌和根际细菌的作用机制进行分离和选择。从松树幼苗中分离出87株细菌，按形态学标准纯化并进行分组。本研究的目的是评估和选择能产生植物生长调节剂、磷酸盐增溶剂、可溶性磷量测定和几丁质酶产生的菌株。37株表现出了至少一个评估活动,与负债的水平fitorreguadores parametros van de 54.4到139µg / ml,周围halos殖民地3.2至3.8公分solubilizando磷酸盐含量varian 0.1至2.4毫克/升和增长完全与周围地区鲜明的殖民地halos 1.2至2厘米为quitinasa负债的。这项工作的结果代表了开发森林植物生长促进系统的第一步
{"title":"Isolation and screening of plant growth promoting bacteria for their application in forest species","authors":"Francisco David Moreno-Valencia, M. Plascencia-Espinosa, J. Rojas","doi":"10.29267/mxjb.2018.3.3.36","DOIUrl":"https://doi.org/10.29267/mxjb.2018.3.3.36","url":null,"abstract":"Se realizo el aislamiento y seleccion segun su mecanismo de accion de bacterias endofiticas y rizosfericas promotoras de crecimiento vegetal de poblaciones silvestres de Pinus patula y Pinus montezumae, grupos arboreos de coniferas mexicanas utilizados con fines de reforestacion por su crecimiento acelerado y alto rendimiento. Ochenta y siete cepas bacterianas fueron aisladas de plantulas de pino, purificadas y agrupadas por criterios morfologicos. Se evaluaron y seleccionaron cepas que producen fitoreguladores de crecimiento, solubilizacion de fosfato, determinacion de la cantidad de fosforo solubilizado y produccion de quitinasa. Treinta y siete cepas mostraron al menos una de las actividades evaluadas, con niveles de produccion de fitorreguadores en parametros que van de 54.4 a 139 µg/ml, halos alrededor de las colonias de 3.2 a 3.8 cm solubilizando fosfato en cantidades que varian de 0.1 a 2.4 mg/L y crecimiento completo con zonas claras alrededor de las colonias con halos de 1.2 a 2 cm para la produccion de quitinasa. Los resultados de este trabajo representan el primer paso para desarrollar un sistema de aceleracion de crecimiento para plantas de uso forestal","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"3 1","pages":"36-53"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.29267/mxjb.2018.3.4.70
Sicar Domínguez-Sánchez, H. Martínez-Montoya, María Cristina Hernández Jiménez, H. F. Torres-Rodríguez, G. Rodríguez-Castillejos
Environmental pollution is one of the biggest concerns facing humanity today, some compounds can be present in soil and aquifers for long periods of time. Additionally, its harmful effects on human health are well known; the environment is capable to self-restore contaminates areas in small concentrations, however those naturals limits of degradation have been exceeded. Thus, its important to implement remediation technologies to treat soils with toxic waste, such as oil derivatives. A wide number of remediation using microorganisms have been evaluated and it is known that microorganisms perform degradation of toxic compounds either alone or in consortiums of multiple organisms.
{"title":"The microorganisms as an alternative approach in remediation of hydrocarbon-contaminated","authors":"Sicar Domínguez-Sánchez, H. Martínez-Montoya, María Cristina Hernández Jiménez, H. F. Torres-Rodríguez, G. Rodríguez-Castillejos","doi":"10.29267/mxjb.2018.3.4.70","DOIUrl":"https://doi.org/10.29267/mxjb.2018.3.4.70","url":null,"abstract":"Environmental pollution is one of the biggest concerns facing humanity today, some compounds can be present in soil and aquifers for long periods of time. Additionally, its harmful effects on human health are well known; the environment is capable to self-restore contaminates areas in small concentrations, however those naturals limits of degradation have been exceeded. Thus, its important to implement remediation technologies to treat soils with toxic waste, such as oil derivatives. A wide number of remediation using microorganisms have been evaluated and it is known that microorganisms perform degradation of toxic compounds either alone or in consortiums of multiple organisms.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"3 1","pages":"70-83"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.29267/mxjb.2018.3.3.68
Estefania del Carmen Urdiana-Arteaga, E. López
Gomphrena globosa is a plant that produces different secondary metabolites that have an interest due to the biological activity they present, for example: anti-inflammatory, cytotoxic, antioxidant and antimicrobial activity. The main metabolites that have been found in in vitro cultures are simple phenols and flavonoids. The biosynthetic pathway of these compounds is well known, which is as a tool for genetic manipulation when there is enough information about their structural genes. However, there is not enough information about its regulation, which opens the field to investigate the correlation between the corresponding gene expression levels and the regulation of the pathway in G. globosa, and the applications in metabolic engineering. Therefore, a bioinformatic analysis was carried out to identify possible candidate enzymes that exert regulation or that could be branch points in the pathway. Nucleotide sequences were obtained from highly related enzymes, as there are no reported sequences for G. globosa enzymes at the moment; however the available information for these related molecules allowed selecting a group of six enzymes as regulation nodes in the biosynthetic pathway of phenylpropanoids in G. globosa. The oligonucleotides designed by sequence homology gathered the design considerations to be used in further gene expression analysis and they are an important contribution to the genetic and metabolic regulation research in G. globosa. Mexican Journal of Biotechnology 2018, 3(3):68-76 Journal homepage:www.mexjbiotechnol.com ISSN:2448-6590 Journal homepage:www.mexjbiotechnol.com ISSN:2448-6590 SHORT COMMUNICATION
{"title":"Bioinformatic analysis of the phenylpropanoid biosynthesis pathway in Gomphrena globosa for the study of gene expression levels","authors":"Estefania del Carmen Urdiana-Arteaga, E. López","doi":"10.29267/mxjb.2018.3.3.68","DOIUrl":"https://doi.org/10.29267/mxjb.2018.3.3.68","url":null,"abstract":"Gomphrena globosa is a plant that produces different secondary metabolites that have an interest due to the biological activity they present, for example: anti-inflammatory, cytotoxic, antioxidant and antimicrobial activity. The main metabolites that have been found in in vitro cultures are simple phenols and flavonoids. The biosynthetic pathway of these compounds is well known, which is as a tool for genetic manipulation when there is enough information about their structural genes. However, there is not enough information about its regulation, which opens the field to investigate the correlation between the corresponding gene expression levels and the regulation of the pathway in G. globosa, and the applications in metabolic engineering. Therefore, a bioinformatic analysis was carried out to identify possible candidate enzymes that exert regulation or that could be branch points in the pathway. Nucleotide sequences were obtained from highly related enzymes, as there are no reported sequences for G. globosa enzymes at the moment; however the available information for these related molecules allowed selecting a group of six enzymes as regulation nodes in the biosynthetic pathway of phenylpropanoids in G. globosa. The oligonucleotides designed by sequence homology gathered the design considerations to be used in further gene expression analysis and they are an important contribution to the genetic and metabolic regulation research in G. globosa. Mexican Journal of Biotechnology 2018, 3(3):68-76 Journal homepage:www.mexjbiotechnol.com ISSN:2448-6590 Journal homepage:www.mexjbiotechnol.com ISSN:2448-6590 SHORT COMMUNICATION","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"3 1","pages":"68-76"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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