Pub Date : 2023-01-16DOI: 10.29267/mxjb.2023.8.1.96
{"title":"Proceedings of the IX International Congress on Biotechnology SOCIBI, III Meeting of Students and Alumni of the CICB-UATx","authors":"","doi":"10.29267/mxjb.2023.8.1.96","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.1.96","url":null,"abstract":"","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45381218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-03DOI: 10.29267/mxjb.2023.8.1.66
Y. García-Esquivel, Y. Mercado-Flores, Miguel Angel Anducho- Reyes, A. Téllez-Jurado
The objective of the work was to evaluate the production of lignocllulolytic enzymes produced by Trametes polyzona HHM001 during its growth on corn leaf residues. Two particle sizes (PS) (PS8 and PS12), two levels of aeration (1 vvm and 0 vvm), as well as the height of the substrate in the production of lignocellulolytic enzymes were tested. The enzymatic activities of Laccase (Lcc), Lignin peroxidase (LiP), and Manganese peroxidase (MnP) were favored under aerated conditions (1 vvm). The enzymatic activity of Lcc was the most favored, with 80 Activity Units (AU)/mL, compared to the culture without aeration, in which 40 AU/mL was obtained. The production of xylanases (Xyl) and cellulases (Cel) were not influenced by aeration under the tested conditions. The results indicated that the particle size has more effect on enzyme production than the presence or absence of air, with a particle size of 8 where the best levels of enzymatic activity were observed. It was observed that the height of the substrate in the fermentation column strongly affects the activity of ligninolytic enzymes and not of the hydrolytic; at 5 cm, the highest ligninolytic activity was detected, where aeration favors oxidative conditions.
{"title":"Effect of particle size, aeration, and substrate height on the production of lignocellulolytic enzymes produced by Trametes polyzona HHM001 grown on corn leaf residues.","authors":"Y. García-Esquivel, Y. Mercado-Flores, Miguel Angel Anducho- Reyes, A. Téllez-Jurado","doi":"10.29267/mxjb.2023.8.1.66","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.1.66","url":null,"abstract":"The objective of the work was to evaluate the production of lignocllulolytic enzymes produced by Trametes polyzona HHM001 during its growth on corn leaf residues. Two particle sizes (PS) (PS8 and PS12), two levels of aeration (1 vvm and 0 vvm), as well as the height of the substrate in the production of lignocellulolytic enzymes were tested. The enzymatic activities of Laccase (Lcc), Lignin peroxidase (LiP), and Manganese peroxidase (MnP) were favored under aerated conditions (1 vvm). The enzymatic activity of Lcc was the most favored, with 80 Activity Units (AU)/mL, compared to the culture without aeration, in which 40 AU/mL was obtained. The production of xylanases (Xyl) and cellulases (Cel) were not influenced by aeration under the tested conditions. The results indicated that the particle size has more effect on enzyme production than the presence or absence of air, with a particle size of 8 where the best levels of enzymatic activity were observed. It was observed that the height of the substrate in the fermentation column strongly affects the activity of ligninolytic enzymes and not of the hydrolytic; at 5 cm, the highest ligninolytic activity was detected, where aeration favors oxidative conditions.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46538119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-03DOI: 10.29267/mxjb.2023.8.1.86
J. A. Núñez-Gastélum, Gabriela Vera-García, Á. Díaz-Sánchez
In this study, the root extract of Cylindropuntia cholla was analyzed as a possible source of substances with potential urease inhibitory and antibacterial activity. C. cholla cactus are abundant in the arid regions of Mexico and are traditionally used to treat kidney diseases, urinary tract infections, and in some cases, to combat the formation of kidney stones. Anti-urease action was evaluated from methanolic root extracts by initial velocity methods. We observed an IC50 of 2.04 mg/mL. Lineweaver-Burk pattern showed an uncompetitive inhibition. The antibacterial activity was evaluated over Klebsiella pneumoniae (ATCCTM 13883) and Staphylococcus aureus (ATCCTM 25923) and we only observed effects over S. aureus growth at 200 mg/mL of extract concentration.
{"title":"Screening of anti-urease and antibacterial activities of root extract from Cylindropuntia cholla","authors":"J. A. Núñez-Gastélum, Gabriela Vera-García, Á. Díaz-Sánchez","doi":"10.29267/mxjb.2023.8.1.86","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.1.86","url":null,"abstract":"In this study, the root extract of Cylindropuntia cholla was analyzed as a possible source of substances with potential urease inhibitory and antibacterial activity. C. cholla cactus are abundant in the arid regions of Mexico and are traditionally used to treat kidney diseases, urinary tract infections, and in some cases, to combat the formation of kidney stones. Anti-urease action was evaluated from methanolic root extracts by initial velocity methods. We observed an IC50 of 2.04 mg/mL. Lineweaver-Burk pattern showed an uncompetitive inhibition. The antibacterial activity was evaluated over Klebsiella pneumoniae (ATCCTM 13883) and Staphylococcus aureus (ATCCTM 25923) and we only observed effects over S. aureus growth at 200 mg/mL of extract concentration.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47864411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-03DOI: 10.29267/mxjb.2023.8.1.1
Karen Guadalupe Quintero-Garrido, F. Ramírez-Montiel, M. Chávez-Castillo, Y. Reyes-Vidal, F. J. Bacame-Valenzuela, F. Padilla‐Vaca, L. Palma-Tirado, M. Estevez, B. L. España Sánchez
The present study describes the antibacterial behavior and the bacterial resistance analysis of extremophile Pseudomonas aeruginosa in contact with copper nanoparticles (CuNPs). For this purpose, green synthesis of CuNPs was performed by combined ultrasound-assisted and chemical reduction methods, obtaining semispherical CuNPs ranging from ca. 4-9 nm. Antibacterial activity (AA) of biosynthesized CuNPs demonstrates an antibacterial inhibition of 85 % (LD85) at 400 μg/mL and a minimum bactericidal concentration (MBC) of 800 μg/mL after 3 h of contact. Bacterial adaptation in contact with CuNPs was observed through the consecutive exposition of microorganisms, presenting a significant increase of LD85 values from 400 μg/mL to 6400 μg/mL after 11 expositions. This behavior demonstrates the bacterial growth adaptation with high-dose of CuNPs. The bacterial resistance mechanism was determined through the overproduction of pyocyanin, associated with oxidative stress events, the genomic polymorphism of resistant bacteria obtained by PCR-RAPDs, and the morphological interaction between P. aeruginosa and CuNPs evidenced by transmission electron microscopy (TEM) micrographs. Our results suggest that under controlled CuNPs exposition, extremophile P. aeruginosa can generate bacterial resistance mechanisms, an important issue for the effective design of antimicrobial nanomaterials.
{"title":"Antibacterial behavior and bacterial resistance analysis of P. aeruginosa in contact with copper nanoparticles","authors":"Karen Guadalupe Quintero-Garrido, F. Ramírez-Montiel, M. Chávez-Castillo, Y. Reyes-Vidal, F. J. Bacame-Valenzuela, F. Padilla‐Vaca, L. Palma-Tirado, M. Estevez, B. L. España Sánchez","doi":"10.29267/mxjb.2023.8.1.1","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.1.1","url":null,"abstract":"The present study describes the antibacterial behavior and the bacterial resistance analysis of extremophile Pseudomonas aeruginosa in contact with copper nanoparticles (CuNPs). For this purpose, green synthesis of CuNPs was performed by combined ultrasound-assisted and chemical reduction methods, obtaining semispherical CuNPs ranging from ca. 4-9 nm. Antibacterial activity (AA) of biosynthesized CuNPs demonstrates an antibacterial inhibition of 85 % (LD85) at 400 μg/mL and a minimum bactericidal concentration (MBC) of 800 μg/mL after 3 h of contact. Bacterial adaptation in contact with CuNPs was observed through the consecutive exposition of microorganisms, presenting a significant increase of LD85 values from 400 μg/mL to 6400 μg/mL after 11 expositions. This behavior demonstrates the bacterial growth adaptation with high-dose of CuNPs. The bacterial resistance mechanism was determined through the overproduction of pyocyanin, associated with oxidative stress events, the genomic polymorphism of resistant bacteria obtained by PCR-RAPDs, and the morphological interaction between P. aeruginosa and CuNPs evidenced by transmission electron microscopy (TEM) micrographs. Our results suggest that under controlled CuNPs exposition, extremophile P. aeruginosa can generate bacterial resistance mechanisms, an important issue for the effective design of antimicrobial nanomaterials.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45799517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-03DOI: 10.29267/mxjb.2023.8.1.21
A. P. Juárez-Rangel, A. Solís-Oba, R. M. Martínez-Cásares, R. Castro-Rivera, M. Solís-Oba
The presence of sargassum on the Caribbean coast causes ecological problems, as does the sludge from water treatment plants. Fertilizers like Nutrkam (sargassum extract) and Biofertex (leachate from the vermicomposting of sludge from a textile plant) have been prepared from these two materials. Both biostimulants were applied to a lettuce crop in a greenhouse, the doses were 2, 5, 7.5, 10 and 20% by volume, the results were compared with urea fertilization. At eight weeks, height, number of green leaves and senescent leaves, fresh and dry weight, antioxidant capacity, and flavonoid content were evaluated. With NutrKam at 5, 10 and 20% and Biofertex at 7.5, 10 and 20%, the height of the leaves was statistically equal to that obtained with urea; there was no significant difference in the weight of leaves when fertilizing with 20% NutrKam, 7.5% Biofertex and urea. With all the treatments, except with Biofertex at 10 %, the antioxidant content was higher than that of lettuces fertilized with urea. The use of both materials to prepare fertilizers is an attractive alternative, since on the one hand the pollution caused by their inadequate disposal is reduced, and on the other, their agricultural application would help reduce the use of chemical fertilizers, without impairing the yield and crop properties.
{"title":"Greenhouse lettuce production fertilized with sargassum extract or vermicompost leachate","authors":"A. P. Juárez-Rangel, A. Solís-Oba, R. M. Martínez-Cásares, R. Castro-Rivera, M. Solís-Oba","doi":"10.29267/mxjb.2023.8.1.21","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.1.21","url":null,"abstract":"The presence of sargassum on the Caribbean coast causes ecological problems, as does the sludge from water treatment plants. Fertilizers like Nutrkam (sargassum extract) and Biofertex (leachate from the vermicomposting of sludge from a textile plant) have been prepared from these two materials. Both biostimulants were applied to a lettuce crop in a greenhouse, the doses were 2, 5, 7.5, 10 and 20% by volume, the results were compared with urea fertilization. At eight weeks, height, number of green leaves and senescent leaves, fresh and dry weight, antioxidant capacity, and flavonoid content were evaluated. With NutrKam at 5, 10 and 20% and Biofertex at 7.5, 10 and 20%, the height of the leaves was statistically equal to that obtained with urea; there was no significant difference in the weight of leaves when fertilizing with 20% NutrKam, 7.5% Biofertex and urea. With all the treatments, except with Biofertex at 10 %, the antioxidant content was higher than that of lettuces fertilized with urea. The use of both materials to prepare fertilizers is an attractive alternative, since on the one hand the pollution caused by their inadequate disposal is reduced, and on the other, their agricultural application would help reduce the use of chemical fertilizers, without impairing the yield and crop properties.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48340614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-03DOI: 10.29267/mxjb.2023.8.1.37
Rubicel Torres-Custodio, M. A. Hernández-Rivera, J. S. López-Lázaro, Y. Córdova-Bautista, J. Alvarez-Ramirez, P. Sifuentes-Gallardo, M. E. Ojeda-Morales
Biosurfactants are metabolites produced by microorganisms during their growth and reproduction. They are amphiphilic molecules capable of modifying surface and interfacial tension. A petrophilic, nitrogen-fixing, phosphorus-solubilizing, and biosurfactant-producing bacterial strain was isolated and characterized. The isolated strain was cultivated in Kim medium with a C:N ratio of 2:1, from which its growth kinetics was determined. Samples of the culture broth were taken at different times and the collapsed drop, oil dispersion and emulsification index tests were performed. Maximum culture growth occurred at 72 h. The biosurfactant was extracted from the crude cell-free broth using a mixture of solvents and a yield of 2.21 g/L was obtained. Thin-layer chromatography and FT-IR analyzes revealed the presence of a lipopeptide biosurfactant. Tensiometry tests showed that the biosurfactant was able to lower the surface tension of water from 72 mN/m to 40 mN/m and the critical micelle concentration was 0.058 (w/w). Probit analysis for toxicity determination showed that there was no weight loss in Eisenia foetida specimens in a range between 10000 and 50000 ppm.
{"title":"Extraction and characterization of a biosurfactant synthesized by Acinotebacter baumannii","authors":"Rubicel Torres-Custodio, M. A. Hernández-Rivera, J. S. López-Lázaro, Y. Córdova-Bautista, J. Alvarez-Ramirez, P. Sifuentes-Gallardo, M. E. Ojeda-Morales","doi":"10.29267/mxjb.2023.8.1.37","DOIUrl":"https://doi.org/10.29267/mxjb.2023.8.1.37","url":null,"abstract":"Biosurfactants are metabolites produced by microorganisms during their growth and reproduction. They are amphiphilic molecules capable of modifying surface and interfacial tension. A petrophilic, nitrogen-fixing, phosphorus-solubilizing, and biosurfactant-producing bacterial strain was isolated and characterized. The isolated strain was cultivated in Kim medium with a C:N ratio of 2:1, from which its growth kinetics was determined. Samples of the culture broth were taken at different times and the collapsed drop, oil dispersion and emulsification index tests were performed. Maximum culture growth occurred at 72 h. The biosurfactant was extracted from the crude cell-free broth using a mixture of solvents and a yield of 2.21 g/L was obtained. Thin-layer chromatography and FT-IR analyzes revealed the presence of a lipopeptide biosurfactant. Tensiometry tests showed that the biosurfactant was able to lower the surface tension of water from 72 mN/m to 40 mN/m and the critical micelle concentration was 0.058 (w/w). Probit analysis for toxicity determination showed that there was no weight loss in Eisenia foetida specimens in a range between 10000 and 50000 ppm.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46348896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-11DOI: 10.29267/mxjb.2022.7.4.37
{"title":"Proceedings of the 3rd Biotechnology World Symposium. 4º Congreso Estudiantil de Ingeniería Biotecnológica SEIBT, XIV Encuentro de Investigadores de la Red de Biotecnología, XXIV Jornadas Académicas del Doctorado en Ciencias en Biotecnología","authors":"","doi":"10.29267/mxjb.2022.7.4.37","DOIUrl":"https://doi.org/10.29267/mxjb.2022.7.4.37","url":null,"abstract":"","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43125457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29267/mxjb.2022.7.4.14
R. Martínez-Pérez, L. Moreno-Vilet
Human beings consume probiotics and prebiotics because of the health benefits they provide. Lactobacillus gasseri is a probiotic microorganism native to humans which produces glycolytic enzymes directed at the hydrolysis of soluble fibers as prebiotic fructans. In this work, levansucrase (LevG) and inulosucrase (InuGB) from L. gasseri were used to predict the ability of L. gasseri to interact with linear and branched fructans prebiotics (inulin and agavins). AlphaFold and SWISS-MODEL were the servers used for tertiary structure prediction of LevG and InuGB, and fructans with different degrees of polymerization (DP2, DP4 DP6, DP8, DP12 and DP20) were used to generate ligand-enzyme molecular dockings by AutodockVina, GlycoTorchVina, and Autodock FR software. The best affinity energies obtained by molecular docking were obtained with agavin and inulin with a DP of 6 and 8 units. Aspartic acid, glutamic acid, asparagine and arginin are the main amino acids involved in the interaction with these substrates. At the same time, the binding pocket shows hydrophilic characteristics; GlycoTorchVina was the best software for protein-ligand docking. These results demonstrate the ability of L. gasseri enzymes to interact with different molecular structures of fructans; levansucrase with better affinity to agavin and inulosucrase with better affinity to inulin. The above helps to understand the structure-functionality relationship of the prebiotic effect, both in symbiotic formulations and in the human digestive tract.
{"title":"Molecular interaction of linear and branched fructans of different sizes with levansucrase and inulosucrase from Lactobacillus gasseri: modeling and computational analysis","authors":"R. Martínez-Pérez, L. Moreno-Vilet","doi":"10.29267/mxjb.2022.7.4.14","DOIUrl":"https://doi.org/10.29267/mxjb.2022.7.4.14","url":null,"abstract":"Human beings consume probiotics and prebiotics because of the health benefits they provide. Lactobacillus gasseri is a probiotic microorganism native to humans which produces glycolytic enzymes directed at the hydrolysis of soluble fibers as prebiotic fructans. In this work, levansucrase (LevG) and inulosucrase (InuGB) from L. gasseri were used to predict the ability of L. gasseri to interact with linear and branched fructans prebiotics (inulin and agavins). AlphaFold and SWISS-MODEL were the servers used for tertiary structure prediction of LevG and InuGB, and fructans with different degrees of polymerization (DP2, DP4 DP6, DP8, DP12 and DP20) were used to generate ligand-enzyme molecular dockings by AutodockVina, GlycoTorchVina, and Autodock FR software. The best affinity energies obtained by molecular docking were obtained with agavin and inulin with a DP of 6 and 8 units. Aspartic acid, glutamic acid, asparagine and arginin are the main amino acids involved in the interaction with these substrates. At the same time, the binding pocket shows hydrophilic characteristics; GlycoTorchVina was the best software for protein-ligand docking. These results demonstrate the ability of L. gasseri enzymes to interact with different molecular structures of fructans; levansucrase with better affinity to agavin and inulosucrase with better affinity to inulin. The above helps to understand the structure-functionality relationship of the prebiotic effect, both in symbiotic formulations and in the human digestive tract.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44559065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29267/mxjb.2022.7.4.1
F. Basurto-Alcántara, R. Lagunes-Quintanilla, Víctor Roldán-Rodríguez, U. Valdez-Espinoza, Ninnet Gómez-Romero
Infection by bovine viral diarrhea virus (BVDV) in cattle remains a source of significant economic losses for livestock producers. This virus is classified within the Pestivirus genus, including three main species: Pestivirus A (BVDV-1), Pestivirus B (BVDV-2), and Pestivirus H (HoBi-like pestivirus). Here, we performed a molecular epidemiological investigation aiming to evaluate the genetic diversity of BVDV in cattle from backyard farms in a municipality in Mexico named Tepalcingo, Morelos, with records of reproductive disorders. RT-PCR was conducted in 111 serum samples from affected cattle. Viral RNA was detected in 47.74% of the samples analyzed. Sequencing and phylogenetic analysis based on 5'UTR showed that the circulating subgenotype was BVDV-1a in all positive samples. These findings reveal the prevalence of BVDV in the surveyed population; thus suggesting a possible association with the previous records of reproductive manifestation in the herd. However, further studies are needed to confirm BVDV as the causative agent. Additionally, our results represent a helpful tool for designing control and prevention strategies accurate to the current regional epidemiological situation. Moreover, obtained information from this type of epidemiological study will assist the implementation of biosafety measures on backyard farms with limited economic resources.
{"title":"Genetic characterization of bovine viral diarrhea virus detected in backyard cattle farms in Mexico","authors":"F. Basurto-Alcántara, R. Lagunes-Quintanilla, Víctor Roldán-Rodríguez, U. Valdez-Espinoza, Ninnet Gómez-Romero","doi":"10.29267/mxjb.2022.7.4.1","DOIUrl":"https://doi.org/10.29267/mxjb.2022.7.4.1","url":null,"abstract":"Infection by bovine viral diarrhea virus (BVDV) in cattle remains a source of significant economic losses for livestock producers. This virus is classified within the Pestivirus genus, including three main species: Pestivirus A (BVDV-1), Pestivirus B (BVDV-2), and Pestivirus H (HoBi-like pestivirus). Here, we performed a molecular epidemiological investigation aiming to evaluate the genetic diversity of BVDV in cattle from backyard farms in a municipality in Mexico named Tepalcingo, Morelos, with records of reproductive disorders. RT-PCR was conducted in 111 serum samples from affected cattle. Viral RNA was detected in 47.74% of the samples analyzed. Sequencing and phylogenetic analysis based on 5'UTR showed that the circulating subgenotype was BVDV-1a in all positive samples. These findings reveal the prevalence of BVDV in the surveyed population; thus suggesting a possible association with the previous records of reproductive manifestation in the herd. However, further studies are needed to confirm BVDV as the causative agent. Additionally, our results represent a helpful tool for designing control and prevention strategies accurate to the current regional epidemiological situation. Moreover, obtained information from this type of epidemiological study will assist the implementation of biosafety measures on backyard farms with limited economic resources.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48618128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-18DOI: 10.29267/mxjb.2022.7.3.15
A. Romero-Rodríguez, H. S. Luna-Zendejas, A. Solís-Oba, R. Castro-Rivera, A. D. Armenta-Bojórquez, M. Solís-Oba
Tomato is one of the agricultural products with the highest economic value in the world, for its production agrochemicals are generally used, however, their excessive use has caused environmental problems. A commercial sargassum extract was evaluated as a fertilizer for tomato cultivation, it was applied at 2 (N2), 5 (N5) and 8% (N8), with and without inoculation (400 spores) of arbuscular mycorrhizal fungi; mineral fertilization (C+) and no fertilization (C-) were used as controls. With C+ the highest number and weight of tomatoes were obtained, but the fruits were mostly small in diameter; with organic fertilization, medium-sized fruits were obtained, and they surpassed those of C- in all the parameters evaluated. The highest content of carotenoids was found in the tomatoes from N2, N5, N8 and NM8, the highest amount of sugar in the fruits from N5, N8 and NM8, the highest maturity and flavor indices in all organically fertilized with and without arbuscular mycorrhizal fungi except NM5. The use of sargassum as a fertilizer is a good alternative to avoid the problems caused by its excessive arrival on the coasts, and it is given added value since a biofertilizer is obtained that helps production and improves the quality of tomatoes.
{"title":"Evaluation of the tomato quality fertilized with sargassum extract from the Mexican Caribbean and mycorrhizae","authors":"A. Romero-Rodríguez, H. S. Luna-Zendejas, A. Solís-Oba, R. Castro-Rivera, A. D. Armenta-Bojórquez, M. Solís-Oba","doi":"10.29267/mxjb.2022.7.3.15","DOIUrl":"https://doi.org/10.29267/mxjb.2022.7.3.15","url":null,"abstract":"Tomato is one of the agricultural products with the highest economic value in the world, for its production agrochemicals are generally used, however, their excessive use has caused environmental problems. A commercial sargassum extract was evaluated as a fertilizer for tomato cultivation, it was applied at 2 (N2), 5 (N5) and 8% (N8), with and without inoculation (400 spores) of arbuscular mycorrhizal fungi; mineral fertilization (C+) and no fertilization (C-) were used as controls. With C+ the highest number and weight of tomatoes were obtained, but the fruits were mostly small in diameter; with organic fertilization, medium-sized fruits were obtained, and they surpassed those of C- in all the parameters evaluated. The highest content of carotenoids was found in the tomatoes from N2, N5, N8 and NM8, the highest amount of sugar in the fruits from N5, N8 and NM8, the highest maturity and flavor indices in all organically fertilized with and without arbuscular mycorrhizal fungi except NM5. The use of sargassum as a fertilizer is a good alternative to avoid the problems caused by its excessive arrival on the coasts, and it is given added value since a biofertilizer is obtained that helps production and improves the quality of tomatoes.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44762550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：