Pub Date : 2024-07-01DOI: 10.29267/mxjb.2024.9.3.36
Angel González-Márquez, Carmen Sánchez
Mycelial growth of Pleurotus ostreatus and Agrocybe aegerita in malt extract broth was evaluated. Laccase, manganese peroxidase (MnP), lignin peroxidase (LiP) and unspecific peroxygenase (UnP) activities were determined in both strains, using biochemical and polyacrylamide gel electrophoresis techniques. No strain showed MnP, LiP, and UnP activities. P. ostreatus had higher biomass production and laccase yield values than those shown by A. aegerita. P. ostreatus produced approximately twice as much biomass (Xmax) compared to A. aegerita (9.12 and 5.27 g/L, respectively). Maximum laccase activity (Emax) was nine-fold higher in P. ostreatus than in A. aegerita (106.5 and 12.23 U/L, respectively). P. ostreatus and A. aegerita showed 3 bands (estimated to be approximately 42, 55, 85 and 212 kDa) and one band (estimated to be approximately 35.2 kDa) with laccase activity, respectively. These results show that laccases are constitutively produced in both fungal species, whereas MnP, LiP and UnP are inducible enzymes. P. ostreatus has a great potential in the biotechnological production of laccases.
{"title":"Mycelial growth and production of laccase and peroxidases by Pleurotus ostreatus and Agrocybe aegerita in liquid fermentation","authors":"Angel González-Márquez, Carmen Sánchez","doi":"10.29267/mxjb.2024.9.3.36","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.3.36","url":null,"abstract":"Mycelial growth of Pleurotus ostreatus and Agrocybe aegerita in malt extract broth was evaluated. Laccase, manganese peroxidase (MnP), lignin peroxidase (LiP) and unspecific peroxygenase (UnP) activities were determined in both strains, using biochemical and polyacrylamide gel electrophoresis techniques. No strain showed MnP, LiP, and UnP activities. P. ostreatus had higher biomass production and laccase yield values than those shown by A. aegerita. P. ostreatus produced approximately twice as much biomass (Xmax) compared to A. aegerita (9.12 and 5.27 g/L, respectively). Maximum laccase activity (Emax) was nine-fold higher in P. ostreatus than in A. aegerita (106.5 and 12.23 U/L, respectively). P. ostreatus and A. aegerita showed 3 bands (estimated to be approximately 42, 55, 85 and 212 kDa) and one band (estimated to be approximately 35.2 kDa) with laccase activity, respectively. These results show that laccases are constitutively produced in both fungal species, whereas MnP, LiP and UnP are inducible enzymes. P. ostreatus has a great potential in the biotechnological production of laccases.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"118 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141695033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.29267/mxjb.2024.9.3.1
J. D. Álvarez-Solís, María del Rosario Cardoso-Villanueva, B. Xoconostle‐Cazares, M. E. Sánchez-García, José Luis Barragán-Soriano, J. Pérez‐Moreno
Tree roots are associated with a high diversity of microorganisms that play key roles in soil fertility and plant nutrition and health. Here we examine the composition and diversity of bacterial community in ectomycorrhizal root (ECR) and their ectomycorrhizosphere (EMR) of the Neotropical tree Pinus montezumae inoculated with Laccaria laccata and Hebeloma mesophaeum, applied alone or co-inoculated with Azospirillum brasilense. For this, we used a 16S rRNA gene metabarcoding-sequencing approach in samples of ECR and EMR that were collected nine-months after inoculation in greenhouse. A total of 943 Operational Taxonomic Units (OTU) were differentiated and grouped in 32 bacterial phyla. The most abundant phyla were Proteobacteria (67%) and Actinobacteria (20.7%). Bacterial communities were structured in ECR and EMR (Anosim, R=0.77, P≤0.005), and were more diverse in EMR than ECR (Shannon-Weaver of 4.2 and 3.6, and Simpson inverse of 33.7 and 16.7, respectively). The abundance of Pseudomonas, Actinoplanes and Salinibacterium were promoted with L. laccata, while with H. mesophaeum an OTU from Burkholderiaceae was prominent. Our results showed that the ectomycorrhizal fungi strongly shaped the composition of the bacterial communities associated with roots of P. montezumae.
树根与微生物的高度多样性有关，这些微生物在土壤肥力、植物营养和健康方面发挥着关键作用。在这里，我们研究了单独或与巴西天青霉（Azospirillum brasilense）共同接种了Laccaria laccata和Hebeloma mesophaeum的新热带树种山榄（Pinus montezumae）的外菌根（ECR）及其外菌根层（EMR）中细菌群落的组成和多样性。为此，我们在温室中接种九个月后采集的 ECR 和 EMR 样品中使用了 16S rRNA 基因代谢编码序列方法。共区分出 943 个操作分类单元（OTU），并将其归入 32 个细菌门。数量最多的菌门是蛋白细菌（67%）和放线菌（20.7%）。ECR和EMR中的细菌群落结构合理（Anosim，R=0.77，P≤0.005），EMR中的细菌群落多样性高于ECR（香农-韦弗指数分别为4.2和3.6，辛普森倒数分别为33.7和16.7）。在 L. laccata 中，假单胞菌、放线菌和盐杆菌的数量增加，而在 H. mesophaeum 中，伯克霍尔德菌科的一个 OTU 则很突出。我们的研究结果表明，外生菌根真菌在很大程度上影响了与山苍子根相关的细菌群落的组成。
{"title":"Bacterial community structure associated with roots of Pinus montezumae Lamb. inoculated with edible ectomycorrhizal fungi and mycorrhizal helper bacteria","authors":"J. D. Álvarez-Solís, María del Rosario Cardoso-Villanueva, B. Xoconostle‐Cazares, M. E. Sánchez-García, José Luis Barragán-Soriano, J. Pérez‐Moreno","doi":"10.29267/mxjb.2024.9.3.1","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.3.1","url":null,"abstract":"Tree roots are associated with a high diversity of microorganisms that play key roles in soil fertility and plant nutrition and health. Here we examine the composition and diversity of bacterial community in ectomycorrhizal root (ECR) and their ectomycorrhizosphere (EMR) of the Neotropical tree Pinus montezumae inoculated with Laccaria laccata and Hebeloma mesophaeum, applied alone or co-inoculated with Azospirillum brasilense. For this, we used a 16S rRNA gene metabarcoding-sequencing approach in samples of ECR and EMR that were collected nine-months after inoculation in greenhouse. A total of 943 Operational Taxonomic Units (OTU) were differentiated and grouped in 32 bacterial phyla. The most abundant phyla were Proteobacteria (67%) and Actinobacteria (20.7%). Bacterial communities were structured in ECR and EMR (Anosim, R=0.77, P≤0.005), and were more diverse in EMR than ECR (Shannon-Weaver of 4.2 and 3.6, and Simpson inverse of 33.7 and 16.7, respectively). The abundance of Pseudomonas, Actinoplanes and Salinibacterium were promoted with L. laccata, while with H. mesophaeum an OTU from Burkholderiaceae was prominent. Our results showed that the ectomycorrhizal fungi strongly shaped the composition of the bacterial communities associated with roots of P. montezumae.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"52 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141696220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.29267/mxjb.2024.9.3.20
Christian Jesús Mora-Pérez, Montserrat Citlali Sánchez-Ruiz, Daniel Salgado-Bautista, U. Carrasco-Navarro, E. Favela‐Torres
Filamentous fungi are widely used in industry to produce enzymes, organic acids, and secondary metabolites. Neurospora crassa has gained attention due to its flexibility for easy genetic manipulation, fast growth, and non-pathogenic characteristics. This study evaluated the growth of N. crassa and invertase production in submerged culture (SC) and solid-state culture (SSC) using pine sawdust (SSC-PS) and polyurethane foam (SSC-PUF) as supports. Modified Vogel's medium with initial sucrose concentrations of 1.5%, 3%, and 5% was used. The specific and maximum CO2 production rates were higher in SSC than SC, particularly with SSC-PS. Protein and invertase production were higher in SSC, with SSC-PS demonstrating the highest sucrose concentration as the inducer and carbon source. The water-holding capacity (WHC) of SSC-PUF was approximately 25 times higher than that of SSC-PS, facilitating a more productive process. SSC-PUF enables higher biomass growth, protein, and invertase production than SC. Moreover, using inert supports, such as PUF, allows the correct substrates and product assessment without interferences due to the chemical composition and heterogeneity of conventional agro-industrial by-products, such as PS.
{"title":"Growth and invertase production by Neurospora crassa in submerged and solid-state cultures","authors":"Christian Jesús Mora-Pérez, Montserrat Citlali Sánchez-Ruiz, Daniel Salgado-Bautista, U. Carrasco-Navarro, E. Favela‐Torres","doi":"10.29267/mxjb.2024.9.3.20","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.3.20","url":null,"abstract":"Filamentous fungi are widely used in industry to produce enzymes, organic acids, and secondary metabolites. Neurospora crassa has gained attention due to its flexibility for easy genetic manipulation, fast growth, and non-pathogenic characteristics. This study evaluated the growth of N. crassa and invertase production in submerged culture (SC) and solid-state culture (SSC) using pine sawdust (SSC-PS) and polyurethane foam (SSC-PUF) as supports. Modified Vogel's medium with initial sucrose concentrations of 1.5%, 3%, and 5% was used. The specific and maximum CO2 production rates were higher in SSC than SC, particularly with SSC-PS. Protein and invertase production were higher in SSC, with SSC-PS demonstrating the highest sucrose concentration as the inducer and carbon source. The water-holding capacity (WHC) of SSC-PUF was approximately 25 times higher than that of SSC-PS, facilitating a more productive process. SSC-PUF enables higher biomass growth, protein, and invertase production than SC. Moreover, using inert supports, such as PUF, allows the correct substrates and product assessment without interferences due to the chemical composition and heterogeneity of conventional agro-industrial by-products, such as PS.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"37 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141701449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-20DOI: 10.29267/mxjb.2024.9.2.51
Edgardo Ocaña-Romo, C. O. Rodríguez-Nava, Carmen Sánchez
Green waste (GW) is generated by the maintenance of public or private green spaces. It is necessary to find ecological alternatives for GW utilization, aiming to avoid accumulation of this material at the environment. In this research, the production of laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and unspecific peroxygenase (UnP) produced by Trametes versicolor grown on GW as a substrate and on polyurethane foam (PUF) as an inert support in solid state fermentation was evaluated. T. versicolor showed higher values of Lac, MnP, UnP and LiP activities (34, 943, 1023 and 766 U/gS, respectively) when grown on GW than when grown on PUF (10.9, 588, 559 and 229 U/gS, respectively). These results suggest that T. versicolor produced Lac inducible and constitutively, while LiP, MnP and UnP were induced by GW at the beginning of fungal growth, however, these enzymes were constitutive and inducible during the rest of the fermentation. The production of oxidases and peroxidases was induced and increased by GW. It is suggested that LiP is involved (as a constitutive enzyme) at the beginning of the exponential phase, while MnP and UnP participate in fungal growth at the end of fermentation. To our knowledge, this is the first detailed study on the main lignincellulose-degrading fungal enzymes involved in GW degradation by fungi. In particular, the relevance of UnP was showed as peroxidase involved in lignocellulosic substrates biodegradation.
{"title":"Oxidases production by Trametes versicolor grown on green waste and on polyurethane foam in solid-state fermentation: A comparative study","authors":"Edgardo Ocaña-Romo, C. O. Rodríguez-Nava, Carmen Sánchez","doi":"10.29267/mxjb.2024.9.2.51","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.2.51","url":null,"abstract":"Green waste (GW) is generated by the maintenance of public or private green spaces. It is necessary to find ecological alternatives for GW utilization, aiming to avoid accumulation of this material at the environment. In this research, the production of laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and unspecific peroxygenase (UnP) produced by Trametes versicolor grown on GW as a substrate and on polyurethane foam (PUF) as an inert support in solid state fermentation was evaluated. T. versicolor showed higher values of Lac, MnP, UnP and LiP activities (34, 943, 1023 and 766 U/gS, respectively) when grown on GW than when grown on PUF (10.9, 588, 559 and 229 U/gS, respectively). These results suggest that T. versicolor produced Lac inducible and constitutively, while LiP, MnP and UnP were induced by GW at the beginning of fungal growth, however, these enzymes were constitutive and inducible during the rest of the fermentation. The production of oxidases and peroxidases was induced and increased by GW. It is suggested that LiP is involved (as a constitutive enzyme) at the beginning of the exponential phase, while MnP and UnP participate in fungal growth at the end of fermentation. To our knowledge, this is the first detailed study on the main lignincellulose-degrading fungal enzymes involved in GW degradation by fungi. In particular, the relevance of UnP was showed as peroxidase involved in lignocellulosic substrates biodegradation.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140681660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-19DOI: 10.29267/mxjb.2024.9.2.19
Franco Ignacio Bugueño-Guerrero, Orlando Catalán-Barrera, M. P. Carballo-Sánchez
Plant growth-promoting rhizobacteria (PGPR) are biostimulants that favor plant development. We evaluated the effect of PGPR in chilli pepper (Capsicum annuum L.) and lettuce (Lactuca sativa L.). An experimental design of 5 treatments and 4 replicates was used for each crop. The bacterial species were Achromobacter xylosoxidans (C56), Arthobacter pokkalii (JLB4), Bacillus pumilus (AV5) and their combination as a consortium. The variables evaluated were: plant height, shoot diameter, relative chlorophyll index (SPAD) and freshweight of aerial part. In these experiments were observed significant differences with C56 to stimulate 11% plant height compared with the other treatments. Bacterial consortium stimulates 7.8% the relative chlorophyll index in lettuce and showing a trend to stimulate freshweight in both plants. The use of PGPR in consortium is an alternative that should be further studied to minimize the use of chemicals, enhance agricultural practicese and mitigate climate change.
{"title":"Effect of a plant growth-promoting rhizobacteria consortium to promote growth in lettuce (Lactuca sativa L.) and chili pepper (Capsicum annuum L.)","authors":"Franco Ignacio Bugueño-Guerrero, Orlando Catalán-Barrera, M. P. Carballo-Sánchez","doi":"10.29267/mxjb.2024.9.2.19","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.2.19","url":null,"abstract":"Plant growth-promoting rhizobacteria (PGPR) are biostimulants that favor plant development. We evaluated the effect of PGPR in chilli pepper (Capsicum annuum L.) and lettuce (Lactuca sativa L.). An experimental design of 5 treatments and 4 replicates was used for each crop. The bacterial species were Achromobacter xylosoxidans (C56), Arthobacter pokkalii (JLB4), Bacillus pumilus (AV5) and their combination as a consortium. The variables evaluated were: plant height, shoot diameter, relative chlorophyll index (SPAD) and freshweight of aerial part. In these experiments were observed significant differences with C56 to stimulate 11% plant height compared with the other treatments. Bacterial consortium stimulates 7.8% the relative chlorophyll index in lettuce and showing a trend to stimulate freshweight in both plants. The use of PGPR in consortium is an alternative that should be further studied to minimize the use of chemicals, enhance agricultural practicese and mitigate climate change.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140683226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-19DOI: 10.29267/mxjb.2024.9.2.34
Laura Lucia Parismoreno-Rivas, Bryan Stalin Barcia-Jalca, R. Medina-Litardo, I. Pérez-Almeida
The establishment of an efficient in vitro propagation protocol is still ongoing for Vanilla tahitensis (J. W. Moore), an underdeveloped but economically attractive crop in Ecuador. The objective of the research was to evaluate the application of different concentrations of cytokine benzylaminopurine (BAP) in its in vitro culture. The effect of six concentrations of BAP was studied (0.50, 1.00, 1.50, 1.75, 2.00 and 2.50 mg/L), and a control treatment, with five repetitions, using a completely randomized design. The 2.0 mg/L concentration of BAP allowed the best micropropagation of the vanilla producing 5 and 6 shoots at 60 and 90 d after sowing. The greatest length of roots was achieved with 1.75 mg/L (7 cm). Regarding plant height and leaf number, the 2.0 mg/L concentration showed the highest plant height with 18 and 6 cm at 90 and 60 d after sowing, and the highest number of leaves with 7 and 5 leaves, respectively. This research reports a successful in vitro culture protocol for this species, to promote the development of this crop in Ecuador, due to its outstanding possibilities for commerce at the international market.
{"title":"Effect of different concentrations of benzylaminopurine for the in vitro propagation of Vanilla tahitensis (J. W. Moore)","authors":"Laura Lucia Parismoreno-Rivas, Bryan Stalin Barcia-Jalca, R. Medina-Litardo, I. Pérez-Almeida","doi":"10.29267/mxjb.2024.9.2.34","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.2.34","url":null,"abstract":"The establishment of an efficient in vitro propagation protocol is still ongoing for Vanilla tahitensis (J. W. Moore), an underdeveloped but economically attractive crop in Ecuador. The objective of the research was to evaluate the application of different concentrations of cytokine benzylaminopurine (BAP) in its in vitro culture. The effect of six concentrations of BAP was studied (0.50, 1.00, 1.50, 1.75, 2.00 and 2.50 mg/L), and a control treatment, with five repetitions, using a completely randomized design. The 2.0 mg/L concentration of BAP allowed the best micropropagation of the vanilla producing 5 and 6 shoots at 60 and 90 d after sowing. The greatest length of roots was achieved with 1.75 mg/L (7 cm). Regarding plant height and leaf number, the 2.0 mg/L concentration showed the highest plant height with 18 and 6 cm at 90 and 60 d after sowing, and the highest number of leaves with 7 and 5 leaves, respectively. This research reports a successful in vitro culture protocol for this species, to promote the development of this crop in Ecuador, due to its outstanding possibilities for commerce at the international market.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" 346","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140682386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.29267/mxjb.2024.9.2.1
Yemitzel Camarero-Arellano, J. Badillo-Corona, Andrea Tovar-Aguilar, N. Durán-Figueroa
MIR genes are genes that give rise to microRNAs (miRNAs), which are small RNAs that regulate key developmental processes such as flowering and embryogenesis. Little is known about its role in the formation of gametes, seeds and fruits. This work demonstrates that the activity of the promoter of the MIR867 gene has a specific expression pattern in male tissue during flower development. Using two T-DNA insertional mutants, a reduction in fruit size (siliques) in length and thickness, and semi-sterility phenotype was demonstrated. To find the cellular reason for the semi-sterility, the Alexander staining technique of pollen grains was used observing that there is defective pollen in the mutant lines. This study is the first report that relates the MIR gene with fruit formation and semi-sterility if seeds in the model plant Arabidopsis thaliana.
MIR基因是产生微RNA（miRNA）的基因，miRNA是一种小RNA，可调控开花和胚胎发生等关键发育过程。人们对其在配子、种子和果实形成过程中的作用知之甚少。这项研究表明，在花的发育过程中，MIR867 基因启动子的活性在雄性组织中有特定的表达模式。利用两个 T-DNA 插入突变体，证明了果实（子房）长度和厚度的减小以及半不育表型。为了找到半不结实的细胞原因，研究人员使用亚历山大染色技术对花粉粒进行了染色，发现突变株的花粉存在缺陷。该研究首次报道了 MIR 基因与模式植物拟南芥果实形成和种子半滞育的关系。
{"title":"The mutation of the MIR867 gene causes seed abortion and affects fruit size in the model plant Arabidopsis thaliana","authors":"Yemitzel Camarero-Arellano, J. Badillo-Corona, Andrea Tovar-Aguilar, N. Durán-Figueroa","doi":"10.29267/mxjb.2024.9.2.1","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.2.1","url":null,"abstract":"MIR genes are genes that give rise to microRNAs (miRNAs), which are small RNAs that regulate key developmental processes such as flowering and embryogenesis. Little is known about its role in the formation of gametes, seeds and fruits. This work demonstrates that the activity of the promoter of the MIR867 gene has a specific expression pattern in male tissue during flower development. Using two T-DNA insertional mutants, a reduction in fruit size (siliques) in length and thickness, and semi-sterility phenotype was demonstrated. To find the cellular reason for the semi-sterility, the Alexander staining technique of pollen grains was used observing that there is defective pollen in the mutant lines. This study is the first report that relates the MIR gene with fruit formation and semi-sterility if seeds in the model plant Arabidopsis thaliana.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140759035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.29267/mxjb.2024.9.1.33
{"title":"Proceedings of the X International Congress on Biotechnology SOCIBI, IV Meeting of Students and Alumni of the CICB-UATx","authors":"","doi":"10.29267/mxjb.2024.9.1.33","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.1.33","url":null,"abstract":"","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139622740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-05DOI: 10.29267/mxjb.2024.9.1.19
Ana Laura López-Escamilla, Viridiana Badillo-Huerta, Patricia Rodríguez-Cuamatzi, J. García-Dávila, Lilia Sánchez-Minutti
Laelia speciosa is an epiphytic orchid endemic to Mexico, enjoying special protected status due to illegal sales and clandestine field extraction. Until now, in vitro cultivation of this plant has not been explored using temporary immersion bioreactor (TIB). L. speciosa seedlings were grown in a TIB, and the presence of bioactive compounds released into the liquid culture medium was determined. Bioactive compounds such as antioxidants, total polyphenols, and flavonoids were identified. The maximum concentration of total polyphenols was 2.29 ± 0.10 mg GAE/g, and the antioxidant capacity reached 33.7 %. Colorimetric tests revealed the presence of flavanones and flavonols. Gas chromatography-mass spectrometry identified components of the culture medium as 1,3-dihydroxyacetone dimer and 5-(hydroxymethyl)-2-furancarbaldehyde. Fourier transform infrared spectroscopy confirmed the presence of polyphenols in the culture medium. This work provides an alternative method for obtaining bioactive compounds while conserving L. speciosa seedlings.
{"title":"Bioactive compounds in Laelia speciosa (Orchidaceae) seedlings grown in temporary immersion bioreactor","authors":"Ana Laura López-Escamilla, Viridiana Badillo-Huerta, Patricia Rodríguez-Cuamatzi, J. García-Dávila, Lilia Sánchez-Minutti","doi":"10.29267/mxjb.2024.9.1.19","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.1.19","url":null,"abstract":"Laelia speciosa is an epiphytic orchid endemic to Mexico, enjoying special protected status due to illegal sales and clandestine field extraction. Until now, in vitro cultivation of this plant has not been explored using temporary immersion bioreactor (TIB). L. speciosa seedlings were grown in a TIB, and the presence of bioactive compounds released into the liquid culture medium was determined. Bioactive compounds such as antioxidants, total polyphenols, and flavonoids were identified. The maximum concentration of total polyphenols was 2.29 ± 0.10 mg GAE/g, and the antioxidant capacity reached 33.7 %. Colorimetric tests revealed the presence of flavanones and flavonols. Gas chromatography-mass spectrometry identified components of the culture medium as 1,3-dihydroxyacetone dimer and 5-(hydroxymethyl)-2-furancarbaldehyde. Fourier transform infrared spectroscopy confirmed the presence of polyphenols in the culture medium. This work provides an alternative method for obtaining bioactive compounds while conserving L. speciosa seedlings.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"6 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139384103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-05DOI: 10.29267/mxjb.2024.9.1.1
Castillo-Minjarez J.M., Vargas-León E.A., Quezada M., Martínez-Valdez F. J.
The impact of inoculum (mature compost) was assessed in an aerobic system for the rapid degradation of organic solid waste, with a focus on the release of reducing sugars. Isothermal (35 and 55 °C) and non-isothermal (35-55 °C) treatments were conducted. Key indicators, including the rate of oxygen consumption (O2) and carbon dioxide production (CO2), were measured to assess microbial activity. Additionally, the concentration of reducing sugars, pH, enzymatic activity, and the presence of pathogens were evaluated. Treatments with inoculum at 35 °C and without inoculum at 55 °C exhibited an increase in oxygen consumption and CO2 production, indicating heightened microbial activity and, consequently, greater mineralization of organic matter. The treatment without inoculum at 35-55 °C presented the highest concentration of reducing sugars (160 mg/g dry weight), suggesting its potential as a pretreatment for bioconversion processes aimed at generating value-added products. At a temperature of 55 °C, there was a reduction of 84.4, 87.28, and 67.33 % in the concentrations of fecal coliforms, Escherichia coli, and parasites, respectively. This result suggests that elevated temperature contributes to the effectiveness of pathogen elimination.
{"title":"Alternative for increasing enzymatic activity and sugar release in organic solid waste during aerobic degradation process","authors":"Castillo-Minjarez J.M., Vargas-León E.A., Quezada M., Martínez-Valdez F. J.","doi":"10.29267/mxjb.2024.9.1.1","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.1.1","url":null,"abstract":"The impact of inoculum (mature compost) was assessed in an aerobic system for the rapid degradation of organic solid waste, with a focus on the release of reducing sugars. Isothermal (35 and 55 °C) and non-isothermal (35-55 °C) treatments were conducted. Key indicators, including the rate of oxygen consumption (O2) and carbon dioxide production (CO2), were measured to assess microbial activity. Additionally, the concentration of reducing sugars, pH, enzymatic activity, and the presence of pathogens were evaluated. Treatments with inoculum at 35 °C and without inoculum at 55 °C exhibited an increase in oxygen consumption and CO2 production, indicating heightened microbial activity and, consequently, greater mineralization of organic matter. The treatment without inoculum at 35-55 °C presented the highest concentration of reducing sugars (160 mg/g dry weight), suggesting its potential as a pretreatment for bioconversion processes aimed at generating value-added products. At a temperature of 55 °C, there was a reduction of 84.4, 87.28, and 67.33 % in the concentrations of fecal coliforms, Escherichia coli, and parasites, respectively. This result suggests that elevated temperature contributes to the effectiveness of pathogen elimination.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"40 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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