Pub Date : 2019-01-01DOI: 10.29267/mxjb.2019.4.2.10
M. A. Cruz-Hernández, J. M. Jiménez-Andrade, A. M. Herrera
La contaminacion ocasionada por hidrocarburos representa un riesgo para el medioambiente. Se han enfocado estudios en la busqueda de alternativas para contribuir a revertir su efecto. Una de ellas involucra el uso de microorganismos nativos capaces de persistir en ambientes contaminados. Por lo cual el objetivo de este trabajo fue analizar el potencial de biotipos de Azospirillum brasilense para degradar compuestos xenobioticos como fenantreno, xileno, tolueno y naftaleno. Se caracterizo la produccion de biosurfactantes, se evaluo in vitro la capacidad de tolerancia a hidrocarburos derivados del petroleo y busqueda de genes relacionados con la degradacion de compuestos aromaticos mediante el programa RAST. Los resultados mostraron que los biotipos produjeron biosurfactantes y se seleccionaron cinco de ellos para realizar ensayos de tolerancia a xileno, tolueno, fenantreno y naftaleno; en los cuales no se observo crecimiento bacteriano despues de 216 horas de incubacion a 30 °C. Se registraron 19 secuencias codificantes relacionadas con la degradacion de compuestos aromaticos; de las cuales 11 estan asociadas con el metabolismo de los compuestos intermedios aromaticos centrales y 5 con las vias catabolicas perifericas cuya funcion se asocia con las rutas de degradacion de quinato, benzoato, salicilato, gentisato y tolueno
{"title":"Characterization of the degradation potential of xenobiotic compounds by the rhizobacteria Azospirillum brasilense","authors":"M. A. Cruz-Hernández, J. M. Jiménez-Andrade, A. M. Herrera","doi":"10.29267/mxjb.2019.4.2.10","DOIUrl":"https://doi.org/10.29267/mxjb.2019.4.2.10","url":null,"abstract":"La contaminacion ocasionada por hidrocarburos representa un riesgo para el medioambiente. Se han enfocado estudios en la busqueda de alternativas para contribuir a revertir su efecto. Una de ellas involucra el uso de microorganismos nativos capaces de persistir en ambientes contaminados. Por lo cual el objetivo de este trabajo fue analizar el potencial de biotipos de Azospirillum brasilense para degradar compuestos xenobioticos como fenantreno, xileno, tolueno y naftaleno. Se caracterizo la produccion de biosurfactantes, se evaluo in vitro la capacidad de tolerancia a hidrocarburos derivados del petroleo y busqueda de genes relacionados con la degradacion de compuestos aromaticos mediante el programa RAST. Los resultados mostraron que los biotipos produjeron biosurfactantes y se seleccionaron cinco de ellos para realizar ensayos de tolerancia a xileno, tolueno, fenantreno y naftaleno; en los cuales no se observo crecimiento bacteriano despues de 216 horas de incubacion a 30 °C. Se registraron 19 secuencias codificantes relacionadas con la degradacion de compuestos aromaticos; de las cuales 11 estan asociadas con el metabolismo de los compuestos intermedios aromaticos centrales y 5 con las vias catabolicas perifericas cuya funcion se asocia con las rutas de degradacion de quinato, benzoato, salicilato, gentisato y tolueno","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"4 1","pages":"10-22"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.29267/mxjb.2019.4.1.61
Dania Fernanda Sánchez-Sánchez, C. Sánchez
Los ésteres de ftalato son compuestos usados principalmente como plastificantes y aditivos para proveer de flexibilidad y maleabilidad a los productos plásticos. Dentro de los ftalatos más empleados se encuentra el dibutil ftalato (DBF), el cual se utiliza como un aditivo en adhesivos, tintas para impresoras y en productos cosméticos. En este trabajo se evaluaron la velocidad específica de crecimiento (μ), biomasa máxima (Xmax), pH, consumo de glucosa y actividad específica de esterasas de Neurospora sitophila en medios adicionados con diferentes concentraciones de DBF (1500 y 2000 mg/L) en fermentación sumergida. N. sitophila presentó una mayor velocidad específica de crecimiento en el medio adicionado con 1500 mg de DBF/L que en el medio adicionado con 2000 mg de DBF/L. El medio adicionado con 2000 mg de DBF/L presentó la mayor producción de biomasa. La mayor actividad específica de esterasas fue observada en el medio conteniendo 1500 mg de DBF/L. Esta investigación sugiere que N. sitophila es capaz de emplear altas concentraciones de DBF como fuente de carbono y energía.
{"title":"Neurospora sitophila crecido en medios adicionados con dibutil ftalato en fermentación sumergida: Cinética de crecimiento y actividad de esterasa","authors":"Dania Fernanda Sánchez-Sánchez, C. Sánchez","doi":"10.29267/mxjb.2019.4.1.61","DOIUrl":"https://doi.org/10.29267/mxjb.2019.4.1.61","url":null,"abstract":"Los ésteres de ftalato son compuestos usados principalmente como plastificantes y aditivos para proveer de flexibilidad y maleabilidad a los productos plásticos. Dentro de los ftalatos más empleados se encuentra el dibutil ftalato (DBF), el cual se utiliza como un aditivo en adhesivos, tintas para impresoras y en productos cosméticos. En este trabajo se evaluaron la velocidad específica de crecimiento (μ), biomasa máxima (Xmax), pH, consumo de glucosa y actividad específica de esterasas de Neurospora sitophila en medios adicionados con diferentes concentraciones de DBF (1500 y 2000 mg/L) en fermentación sumergida. N. sitophila presentó una mayor velocidad específica de crecimiento en el medio adicionado con 1500 mg de DBF/L que en el medio adicionado con 2000 mg de DBF/L. El medio adicionado con 2000 mg de DBF/L presentó la mayor producción de biomasa. La mayor actividad específica de esterasas fue observada en el medio conteniendo 1500 mg de DBF/L. Esta investigación sugiere que N. sitophila es capaz de emplear altas concentraciones de DBF como fuente de carbono y energía.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.29267/MXJB.2019.4.1.51
Naomi Surellem Ríos-González, Ángel González-Márquez, C. Sánchez
Di (2-ethyl hexyl) phthalate (DEHP) is a plasticizer present in various products, mainly those made with PVC. This phthalate has attracted attention due to its important participation in the contamination of the environment. It has been reported as an endocrine-disrupting compound in mammals. Fusarium culmorum is a phytopathogenic fungus able to degrade DEHP, because it produces esterases, which are enzymes capable to break down ester bonds present in the structure of phthalates. In this research, growth, protein content and esterases activity by biochemical tests and polyacrylamide gel electrophoresis were characterized for F. culmorum grown in DEHP-supplemented (100 and 1500 mg/L) media as the only carbon source in liquid fermentation. F. culmorum showed higher biomass production and esterase activity in medium supplemented with 1500 mg of DEHP/L. Zymography revealed that bands with esterase activity were observed after 24 h and 48 h in media supplemented with 1500 and 100 mg of DEHP/L, respectively. It was shown that DEHP is an inducer of esterases and that this compound was used as carbon and energy sources by F. culmorum. This fungus can secrete specific esterase to breakdown high concentrations of DEHP, being a promising organism for bioremediation of DEHP-polluted environments in both aquatic and terrestrial ecosystems.
{"title":"Growth and esterase activity of Fusarium culmorum grown in di(2- ethyl hexyl) phthalate in liquid fermentation","authors":"Naomi Surellem Ríos-González, Ángel González-Márquez, C. Sánchez","doi":"10.29267/MXJB.2019.4.1.51","DOIUrl":"https://doi.org/10.29267/MXJB.2019.4.1.51","url":null,"abstract":"Di (2-ethyl hexyl) phthalate (DEHP) is a plasticizer present in various products, mainly those made with PVC. This phthalate has attracted attention due to its important participation in the contamination of the environment. It has been reported as an endocrine-disrupting compound in mammals. Fusarium culmorum is a phytopathogenic fungus able to degrade DEHP, because it produces esterases, which are enzymes capable to break down ester bonds present in the structure of phthalates. In this research, growth, protein content and esterases activity by biochemical tests and polyacrylamide gel electrophoresis were characterized for F. culmorum grown in DEHP-supplemented (100 and 1500 mg/L) media as the only carbon source in liquid fermentation. F. culmorum showed higher biomass production and esterase activity in medium supplemented with 1500 mg of DEHP/L. Zymography revealed that bands with esterase activity were observed after 24 h and 48 h in media supplemented with 1500 and 100 mg of DEHP/L, respectively. It was shown that DEHP is an inducer of esterases and that this compound was used as carbon and energy sources by F. culmorum. This fungus can secrete specific esterase to breakdown high concentrations of DEHP, being a promising organism for bioremediation of DEHP-polluted environments in both aquatic and terrestrial ecosystems.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.29267/mxjb.2018.3.4.1
J. M. González-Alvarado, Tania Gómez-Hernández, Nohemi Hernández-Camacho, Edmundo W. Sánchez-Márquez, Antonio J. Soto-Sánchez, Marco Antonio Cruz-Portillo, J. Aragón-Hernández, Adelfo Vite-Aranda, J. Yáñez-Hernández
Se evaluó el efecto de la suplementación con metionina de cromo (Met-Cr) sobre el desempeño productivo y características de la canal en cerdos en finalización. Se evaluaron 3 tratamientos; 1) testigo en donde los cerdos fueron alimentados con dietas de finalización de 60 a 100 kg; 2) testigo + 0.8 mg de Cr/kg de alimento desde 60 hasta 100 kg; y 3) testigo + 0.8 mg de Cr/kg de alimento desde 80 hasta 100 kg. Los cerdos suplementados con MetCr consumieron menos alimento (P≤0.05), crecieron más rápido (P≤0.05) y fueron más pesados (P≤0.05) y más eficientes (P≤0.05) que los cerdos testigo, pero el efecto benéfico fue mayor cuando la suplementación inició a menor peso. La adición de Met-Cr incrementó el área del músculo Longissimus dorsi (P≤0.01), el rendimiento de canal (P≤0.01) y redujo el espesor de grasa dorsal (P≤0.01). Además, la carne de cerdos suplementados presentó valores de pH y luminosidad (L*) más bajos (P≤0.01) que de cerdos testigo. Incluso, el consumo de Met-Cr redujo las pérdidas a la cocción (P≤0.01) y la fuerza de corte (P≤0.01), pero el efecto sobre la suavidad fue mayor al suplementar a menor peso. En conclusión, la suplementación de Met-Cr en cerdos mejora el desempeño productivo, las características de la canal y algunos indicadores de calidad de la carne, pero la etapa en que inicia la suplementación con Cr puede afectar la respuesta animal.
{"title":"Desempeño productivo, características de la canal y calidad de la carne de cerdos alimentados con dietas base sorgo-pasta de soya adicionadas con metionina de cromo en diferentes etapas de desarrollo","authors":"J. M. González-Alvarado, Tania Gómez-Hernández, Nohemi Hernández-Camacho, Edmundo W. Sánchez-Márquez, Antonio J. Soto-Sánchez, Marco Antonio Cruz-Portillo, J. Aragón-Hernández, Adelfo Vite-Aranda, J. Yáñez-Hernández","doi":"10.29267/mxjb.2018.3.4.1","DOIUrl":"https://doi.org/10.29267/mxjb.2018.3.4.1","url":null,"abstract":"Se evaluó el efecto de la suplementación con metionina de cromo (Met-Cr) sobre el desempeño productivo y características de la canal en cerdos en finalización. Se evaluaron 3 tratamientos; 1) testigo en donde los cerdos fueron alimentados con dietas de finalización de 60 a 100 kg; 2) testigo + 0.8 mg de Cr/kg de alimento desde 60 hasta 100 kg; y 3) testigo + 0.8 mg de Cr/kg de alimento desde 80 hasta 100 kg. Los cerdos suplementados con MetCr consumieron menos alimento (P≤0.05), crecieron más rápido (P≤0.05) y fueron más pesados (P≤0.05) y más eficientes (P≤0.05) que los cerdos testigo, pero el efecto benéfico fue mayor cuando la suplementación inició a menor peso. La adición de Met-Cr incrementó el área del músculo Longissimus dorsi (P≤0.01), el rendimiento de canal (P≤0.01) y redujo el espesor de grasa dorsal (P≤0.01). Además, la carne de cerdos suplementados presentó valores de pH y luminosidad (L*) más bajos (P≤0.01) que de cerdos testigo. Incluso, el consumo de Met-Cr redujo las pérdidas a la cocción (P≤0.01) y la fuerza de corte (P≤0.01), pero el efecto sobre la suavidad fue mayor al suplementar a menor peso. En conclusión, la suplementación de Met-Cr en cerdos mejora el desempeño productivo, las características de la canal y algunos indicadores de calidad de la carne, pero la etapa en que inicia la suplementación con Cr puede afectar la respuesta animal.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44289209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.29267/MXJB.2018.3.4.33
María de los Ángeles Romero-Bañuelos, Adán Topiltzin Morales-Vargas, Varinia López Ramírez
La industria petroquímica ha afectado de forma negativa al medio ambiente por los compuestos contaminantes que emite, entre ellos se encuentran los hidrocarburos, los cuales se consideran tóxicos. Sin embargo, la biorremediación es una alternativa viable para la descontaminación de sitios afectados por estos compuestos, la bioaumentación, por ejemplo, emplea microorganismos con capacidad degradadora y logra disminuir la concentración de los compuestos contaminantes. En este estudio se diseñaron seis consorcios microbianos a través de ensayos de confrontación entre cepas aisladas del Río Lerma de Salamanca (Guanajuato, México) y de una toma clandestina de gasolina en Pueblo Nuevo, Guanajuato. Los consorcios evaluados presentaron capacidad de degradar hidrocarburos, dos consorcios particularmente fueron seleccionados por su capacidad para degradar naftaleno y diésel. Los consorcios microbianos diseñados en este trabajo, se proponen para que en un futuro puedan ser aplicados en biorremediación de suelos in situ.
{"title":"Diseño y aplicación in vitro de consorcios microbianos degradadores de hidrocarburos","authors":"María de los Ángeles Romero-Bañuelos, Adán Topiltzin Morales-Vargas, Varinia López Ramírez","doi":"10.29267/MXJB.2018.3.4.33","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.4.33","url":null,"abstract":"La industria petroquímica ha afectado de forma negativa al medio ambiente por los compuestos contaminantes que emite, entre ellos se encuentran los hidrocarburos, los cuales se consideran tóxicos. Sin embargo, la biorremediación es una alternativa viable para la descontaminación de sitios afectados por estos compuestos, la bioaumentación, por ejemplo, emplea microorganismos con capacidad degradadora y logra disminuir la concentración de los compuestos contaminantes. En este estudio se diseñaron seis consorcios microbianos a través de ensayos de confrontación entre cepas aisladas del Río Lerma de Salamanca (Guanajuato, México) y de una toma clandestina de gasolina en Pueblo Nuevo, Guanajuato. Los consorcios evaluados presentaron capacidad de degradar hidrocarburos, dos consorcios particularmente fueron seleccionados por su capacidad para degradar naftaleno y diésel. Los consorcios microbianos diseñados en este trabajo, se proponen para que en un futuro puedan ser aplicados en biorremediación de suelos in situ.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42573901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.29267/MXJB.2018.3.4.44
Angela Chen, B. Keitz, Lydia M. Contreras
There is rising interest in nanoparticle biosynthesis using bacteria due to the potential for applications in bioremediation, catalysis, or as antimicrobials. However, biosynthesis remains limited by the inability to control nanoparticle morphology and size due to the lack of knowledge regarding explicit molecular mechanisms. Due to their importance in nanoparticle biosynthesis and as antimicrobials, we focus our discussion on silver, gold, and copper nanoparticles. We discuss recent efforts to elucidate reduction mechanisms that have identified generic enzymes and metal resistance genes as strong candidates to facilitate nanoparticle biosynthesis. Although it is known that these enzymes and genes play significant roles in maintaining bacterial homeostasis, there are few reports discussing this topic. Thus, we discuss examples of how metal resistance genes are conserved across bacteria and have been shown to be important for both nanoparticle biosynthesis and processes such as virulence or oxidative stress responses. Overall, this review highlights biological connections between nanoparticle biosynthesis and stress responses by examining the role of reductases and metal resistance genes in both processes. This understanding provides a greater role for nanoparticle biosynthesis in bacteria and could enable a systems biology level of control over nanoparticle biosynthesis.
{"title":"Biological links between nanoparticle biosynthesis and stress responses in bacteria","authors":"Angela Chen, B. Keitz, Lydia M. Contreras","doi":"10.29267/MXJB.2018.3.4.44","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.4.44","url":null,"abstract":"There is rising interest in nanoparticle biosynthesis using bacteria due to the potential for applications in bioremediation, catalysis, or as antimicrobials. However, biosynthesis remains limited by the inability to control nanoparticle morphology and size due to the lack of knowledge regarding explicit molecular mechanisms. Due to their importance in nanoparticle biosynthesis and as antimicrobials, we focus our discussion on silver, gold, and copper nanoparticles. We discuss recent efforts to elucidate reduction mechanisms that have identified generic enzymes and metal resistance genes as strong candidates to facilitate nanoparticle biosynthesis. Although it is known that these enzymes and genes play significant roles in maintaining bacterial homeostasis, there are few reports discussing this topic. Thus, we discuss examples of how metal resistance genes are conserved across bacteria and have been shown to be important for both nanoparticle biosynthesis and processes such as virulence or oxidative stress responses. Overall, this review highlights biological connections between nanoparticle biosynthesis and stress responses by examining\u0000the role of reductases and metal resistance genes in both processes. This understanding provides a greater role for nanoparticle biosynthesis in bacteria and could enable a systems biology level of control over nanoparticle biosynthesis.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46208474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.29267/MXJB.2018.3.4.19
Hebert Jair Barrales-Cureño, C. Reyes-Reyes, Maximino Díaz-Bautista, A. D. Jesús, Salvador Chávez-Salinas, L. G. López-Valdez
The interferon (IFN) is a cytokine produced by immunocompetent cells in response to various stimuli. Five types of IFNs are identified: alpha, beta, gamma, tau and omega 1. In particular, interferons inhibit viral replication directly by antiviral mechanisms, as they do so indirectly by amplifying immune responses to viral proteins. Likewise, they are also essential elements in clinical oncology. They are used in the treatment of chronic myelocytic and hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphoma, melanoma, renal carcinoma, and Kaposi's sarcoma, as well as in the papilloma. They are also used in diseases of viral origin such as hepatitis B and C, acute respiratory syndrome, and HIV (experimentally in the latter), as well as in neurodegenerative diseases, including multiple sclerosis or amyotrophic lateral sclerosis. The combination of interferons with other therapies is contributing to enhance its antiviral and antitumor activity. This work aims to present an updated reference on the use of interferons in the medical field.
{"title":"Therapeutic effects of interferons in human viral infections","authors":"Hebert Jair Barrales-Cureño, C. Reyes-Reyes, Maximino Díaz-Bautista, A. D. Jesús, Salvador Chávez-Salinas, L. G. López-Valdez","doi":"10.29267/MXJB.2018.3.4.19","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.4.19","url":null,"abstract":"The interferon (IFN) is a cytokine produced by immunocompetent cells in response to various stimuli. Five types of IFNs are identified: alpha, beta, gamma, tau and omega 1. In particular, interferons inhibit viral replication directly by antiviral mechanisms, as they do so indirectly by amplifying immune responses to viral proteins. Likewise, they are also essential elements in clinical oncology. They are used in the treatment of chronic myelocytic and hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphoma, melanoma, renal carcinoma, and Kaposi's sarcoma, as well as in the papilloma. They are also used in diseases of viral origin such as hepatitis B and C, acute respiratory syndrome, and HIV (experimentally in the latter), as well as in neurodegenerative diseases, including multiple sclerosis or amyotrophic lateral sclerosis. The combination of interferons with other therapies is contributing to enhance its antiviral and antitumor activity. This work aims to present an updated reference on the use of interferons in the medical field.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47890573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.29267/MXJB.2018.3.3.87
Rocío Requena-Castro, María Guadalupe Aguilera-Arreola, A. V. Martínez-Vázquez, Virgilio Bocanegra-García
El objetivo de este trabajo fue determinar la presencia de genes de virulencia en cepas de E. coli aisladas del Río Bravo en la ciudad de Reynosa Tamaulipas. Mediante la técnica de PCR se realizó la detección de los genes que codifican para factores de virulencia como son eae, bfp, stx1 y stx2 en muestras de aguas superficiales del Río Bravo en el municipio de Reynosa Tamaulipas y se identificó que el gen stx2 (27%) fue el más prevalente, seguido de stx1 (10%). Aunque el resto de las muestras fueron consideradas comensales al no tener ninguno de los genes propuestos, la presencia de genes stx1 y stx2 son considerados como un riesgo a la población que tiene contacto con el agua superficial del Río Bravo, por lo cual es necesario un monitoreo microbiológico en esta zona.
{"title":"Prevalencia de genes de virulencia de Escherichia coli en aguas superficiales del Río Bravo en la ciudad de Reynosa, Tamaulipas","authors":"Rocío Requena-Castro, María Guadalupe Aguilera-Arreola, A. V. Martínez-Vázquez, Virgilio Bocanegra-García","doi":"10.29267/MXJB.2018.3.3.87","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.3.87","url":null,"abstract":"El objetivo de este trabajo fue determinar la presencia de genes de virulencia en cepas de E. coli aisladas del Río Bravo en la ciudad de Reynosa Tamaulipas. Mediante la técnica de PCR se realizó la detección de los genes que codifican para factores de virulencia como son eae, bfp, stx1 y stx2 en muestras de aguas superficiales del Río Bravo en el municipio de Reynosa Tamaulipas y se identificó que el gen stx2 (27%) fue el más prevalente, seguido de stx1 (10%). Aunque el resto de las muestras fueron consideradas comensales al no tener ninguno de los genes propuestos, la presencia de genes stx1 y stx2 son considerados como un riesgo a la población que tiene contacto con el agua superficial del Río Bravo, por lo cual es necesario un monitoreo microbiológico en esta zona.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43110172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.29267/MXJB.2018.3.3.54
N. Rojas-Avelizapa, Itzayana V. Hipólito-Júarez, M. Gómez-Ramírez
The high levels of toxicity generated by the heavy metal content in industrial wastes has generated environmental and health concerns. One of the strategies to reduce the metallic load is the use of sulfur-oxidizing bacteria, due to its ability to produce sulfuric acid involved in the metal leaching. The aim of this research was to evaluate the growth of Acidithioobacillus thiooxidans DSM 26636 and its ability to leach metals from slags and ashes from coal combustion wastes. Microbial growth was monitoring by sulfate and sulfuric acid production. The metal content in slags and fly ashes was determined by ICPOES. The experiments were carried out during 21 days at 30°C, 150 rpm in 125 mL Erlenmeyer flasks containing 30 mL of Starkey media added with 1% (w/v) of elemental sulfur and 1% (w/v) of slags or ashes. Results showed that Acidithioobacillus thiooxidans was able to leach V, Fe, Mg, Al, Si and Ni from slags. For fly ashes, metal leaching was Al, Ni, Sn, Mg, Zn and Si. Summarizing, Acidithioobacillus thiooxidans could be used for the leaching of different metals contained in wastes from carbon combustion plant.
{"title":"Biological treatment of coal combustion wastes by Acidithiobacillus thiooxidans DSM 26636","authors":"N. Rojas-Avelizapa, Itzayana V. Hipólito-Júarez, M. Gómez-Ramírez","doi":"10.29267/MXJB.2018.3.3.54","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.3.54","url":null,"abstract":"The high levels of toxicity generated by the heavy metal content in industrial wastes has generated environmental and health concerns. One of the strategies to reduce the metallic load is the use of sulfur-oxidizing bacteria, due to its ability to produce sulfuric acid involved in the metal leaching. The aim of this research was to evaluate the growth of Acidithioobacillus thiooxidans DSM 26636 and its ability to leach metals from slags and ashes from coal combustion wastes. Microbial growth was monitoring by sulfate and\u0000sulfuric acid production. The metal content in slags and fly ashes was determined by ICPOES. The experiments were carried out during 21 days at 30°C, 150 rpm in 125 mL Erlenmeyer flasks containing 30 mL of Starkey media added with 1% (w/v) of elemental sulfur and 1% (w/v) of slags or ashes. Results showed that Acidithioobacillus thiooxidans was able to leach V, Fe, Mg, Al, Si and Ni from slags. For fly ashes, metal leaching was Al, Ni, Sn, Mg, Zn and Si. Summarizing, Acidithioobacillus thiooxidans could be used for the leaching of different metals contained in wastes from carbon combustion plant.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43979548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.29267/MXJB.2018.3.3.15
I. A. Rodríguez, Nancy Cecilia Pacheco-Castillo, J. Cárdenas-González, M. Zárate, V. Martínez-Juárez, A. Rodríguez-Pérez
Mercury (II) removal capacity in aqueous solution by Aspergillus niger biomass was analyzed by the atomic absorption spectrometry method. The fungus grew in 2000 ppm of the metal (20.3%). Biosorption was evaluated at different pH (3.5, 4.5, and 5.5) at different times. In addition, the effect of temperature in the range of 28°C to 45oC and removal at different initial concentrations of Hg (II) from 100 to 500 mg/L were also studied. The highest biosorption (83.2% with 100 mg/L of the metal, and 1 g of biomass) was 24 h at pH of 5.5 and 28oC. With regard to temperature, the highest removal was to 28oC, with an 83.2% removal at 24 h, and at higher biomass concentrations, the removal was most efficient (100% in 12 h with 5 g of biomass). Fungal biomass showed good removal capacity of the metal in situ, 69% removal in contaminated water, after 7 days of incubation and 5 g of biomass (100 mL water), so it can be used to remove industrial wastewater.
{"title":"Biosorption of mercury (II) from aqueous solution onto biomass of Aspergillus niger","authors":"I. A. Rodríguez, Nancy Cecilia Pacheco-Castillo, J. Cárdenas-González, M. Zárate, V. Martínez-Juárez, A. Rodríguez-Pérez","doi":"10.29267/MXJB.2018.3.3.15","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.3.15","url":null,"abstract":"Mercury (II) removal capacity in aqueous solution by Aspergillus niger biomass was analyzed by the atomic absorption spectrometry method. The fungus grew in 2000 ppm of the metal (20.3%). Biosorption was evaluated at different pH (3.5, 4.5, and 5.5) at different times. In addition, the effect of temperature in the range of 28°C to 45oC and removal at different initial concentrations of Hg (II) from 100 to 500 mg/L were also studied. The highest biosorption (83.2% with 100 mg/L of the metal, and 1 g of biomass) was 24 h at pH of 5.5 and 28oC. With regard to temperature, the highest removal was to 28oC, with an 83.2% removal at 24 h, and at higher biomass concentrations, the removal was most efficient (100% in 12 h with 5 g of biomass). Fungal biomass showed good removal capacity of the metal in situ, 69% removal in contaminated water, after 7 days of incubation and 5 g of biomass (100 mL water), so it can be used to remove industrial wastewater.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47871748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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