Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-174-189
E. Ulyanovskaya, I. Suprun, T. Bogdanovich, E. Chernutskaya, S. V. Tokmakov, G. Talovina
Background. The development of genetic certificates and nomenclatural standards for newly released cultivars of Malus domestica Borkh. (apple tree) ensures an opportunity to preserve the original and authentic genetic and morphological information on domestic cultivars promising for the south of Russia. The purpose of this study was to develop genetic certificates and nomenclatural standards for 7 apple-tree cultivars developed at the North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making (NCFSCHVW), five of which were released jointly with the All-Russian Research Institute of Fruit Crop Breeding (VNIISPK).Materials and methods. The materials included apple-tree cultivars resistant or immune to scab (Venturia inaequalis (Cooke) G. Winter) from the NCFSCHVW collection stock. Conventional herbarization and DNA fingerprinting methods were used. For cultivar certification, an improved technique of DNA sampling and 12 microsatellite markers were applied.Results. Unique DNA profile certificates and nomenclatural standards were produced for 7 apple-tree cultivars: ‘Vasilisa’, ‘Karmen’, ‘Krasny Yantar’, ‘Soyuz’ and ‘Rassvet’ (with the Rvi6 gene) released jointly by the NCFSCHVW and VNIISPK, plus ‘Zolotoye Letneye’ and ‘Feya’ developed at the NCFSCHVW. The apple-tree herbarium specimens were placed for perpetual storage in the WIR Herbarium of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR).Conclusion. The results obtained are promising for identification and authenticity verification of new domestic apple-tree cultivars during their reproduction and cultivation.
背景。为新发布的 Malus domestica Borkh.（苹果树）栽培品种制定遗传证书和命名标准可确保为俄罗斯南部地区保存国内栽培品种原始、真实的遗传和形态信息。本研究的目的是为北高加索联邦园艺、葡萄栽培和酿酒科学中心（NCFSCHVW）培育的 7 个苹果树栽培品种制定遗传证书和命名标准，其中 5 个品种是与全俄果树育种研究所（VNIISPK）联合推出的。材料包括来自全俄果树作物育种研究所（NCFSCHVW）的抗疮痂病（Venturia inaequalis (Cooke) G. Winter）或免疫疮痂病的苹果树栽培品种。采用了传统的除草和 DNA 指纹方法。为了进行栽培品种认证，采用了一种改进的 DNA 采样技术和 12 个微卫星标记。为 7 个苹果树栽培品种制作了独特的 DNA 图谱证书和命名标准：这些品种包括：由国家林业科学和技术中心（NCFSCHVW）和国家果树研究所（VNIISPK）联合推出的 "Vasilisa"、"Karmen"、"Krasny Yantar"、"Soyuz "和 "Rassvet"（带有 Rvi6 基因），以及由国家林业科学和技术中心（NCFSCHVW）培育的 "Zolotoye Letneye "和 "Feya"。苹果树标本被永久保存在 N.I. Vavilov 全俄植物遗传资源研究所（VIR）的 WIR 标本馆中。所获结果有望在国内苹果树新品种的繁殖和栽培过程中对其进行鉴定和真实性验证。
{"title":"Nomenclatural standards and genetic certificates for apple-tree cultivars developed at the North Caucasian Federal Scientific Center of Horticulture, Viticulture, wine-making","authors":"E. Ulyanovskaya, I. Suprun, T. Bogdanovich, E. Chernutskaya, S. V. Tokmakov, G. Talovina","doi":"10.30901/2227-8834-2023-4-174-189","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-174-189","url":null,"abstract":"Background. The development of genetic certificates and nomenclatural standards for newly released cultivars of Malus domestica Borkh. (apple tree) ensures an opportunity to preserve the original and authentic genetic and morphological information on domestic cultivars promising for the south of Russia. The purpose of this study was to develop genetic certificates and nomenclatural standards for 7 apple-tree cultivars developed at the North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making (NCFSCHVW), five of which were released jointly with the All-Russian Research Institute of Fruit Crop Breeding (VNIISPK).Materials and methods. The materials included apple-tree cultivars resistant or immune to scab (Venturia inaequalis (Cooke) G. Winter) from the NCFSCHVW collection stock. Conventional herbarization and DNA fingerprinting methods were used. For cultivar certification, an improved technique of DNA sampling and 12 microsatellite markers were applied.Results. Unique DNA profile certificates and nomenclatural standards were produced for 7 apple-tree cultivars: ‘Vasilisa’, ‘Karmen’, ‘Krasny Yantar’, ‘Soyuz’ and ‘Rassvet’ (with the Rvi6 gene) released jointly by the NCFSCHVW and VNIISPK, plus ‘Zolotoye Letneye’ and ‘Feya’ developed at the NCFSCHVW. The apple-tree herbarium specimens were placed for perpetual storage in the WIR Herbarium of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR).Conclusion. The results obtained are promising for identification and authenticity verification of new domestic apple-tree cultivars during their reproduction and cultivation.","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":" 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-153-162
N. N. Nuzhdina, D. A. Zhiganov, T. Ermolaeva, T. B. Kulevatova, L. N. Zlobina, L. V. Andreeva, V. A. Kulikova, N. A. Salmanova, V. N. Nechaev
Background. Grain quality indicators of winter rye cultivars were analyzed in connection with the size-dependent fractional composition of their grain in the Lower Volga environments.Materials and methods. The research material included 22 cultivars developed at various Russian scientific institutions, 3 Belarusian ones, and 3 cultivars bred at the Federal Center of Agriculture Research of the South-East Region. Comparative trials were conducted in 2021–2022 on 13.2 m2 plots in two field replications. The following grain quality indicators were studied: grain test weight, 1000 grain weight, size-dependent fractional composition, Hagberg falling number, amylogram peak height, starch gelatinization temperature, protein content, and rheological properties of the meal/water slurry.Results. Protein content was recorded at a level of 9.98–10.85%, while the starch gelatinization temperature was high due to the June air temperatures higher than the norm. Significant differences among the cultivars were found in the fractional composition of grain: 12 forms were identified with medium viscosity of the meal/water slurry and the fine-grain fraction content up to 30% of the total weight.Conclusions. There is a positive significant correlation between the five studied indicators characterizing the rheological properties of the meal/water slurry made from the grain of winter rye cultivars and the grain fraction composition (0.82*–0.91*).
{"title":"Grain quality indicators and size-dependent fractional composition in winter rye cultivars under the conditions of the Lower Volga region","authors":"N. N. Nuzhdina, D. A. Zhiganov, T. Ermolaeva, T. B. Kulevatova, L. N. Zlobina, L. V. Andreeva, V. A. Kulikova, N. A. Salmanova, V. N. Nechaev","doi":"10.30901/2227-8834-2023-4-153-162","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-153-162","url":null,"abstract":"Background. Grain quality indicators of winter rye cultivars were analyzed in connection with the size-dependent fractional composition of their grain in the Lower Volga environments.Materials and methods. The research material included 22 cultivars developed at various Russian scientific institutions, 3 Belarusian ones, and 3 cultivars bred at the Federal Center of Agriculture Research of the South-East Region. Comparative trials were conducted in 2021–2022 on 13.2 m2 plots in two field replications. The following grain quality indicators were studied: grain test weight, 1000 grain weight, size-dependent fractional composition, Hagberg falling number, amylogram peak height, starch gelatinization temperature, protein content, and rheological properties of the meal/water slurry.Results. Protein content was recorded at a level of 9.98–10.85%, while the starch gelatinization temperature was high due to the June air temperatures higher than the norm. Significant differences among the cultivars were found in the fractional composition of grain: 12 forms were identified with medium viscosity of the meal/water slurry and the fine-grain fraction content up to 30% of the total weight.Conclusions. There is a positive significant correlation between the five studied indicators characterizing the rheological properties of the meal/water slurry made from the grain of winter rye cultivars and the grain fraction composition (0.82*–0.91*).","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-205-214
N. Mironenko, N. Kovalenko, O. A. Baranova, O. Mitrofanova
Background. The most effective and environmentally safe way to combat wheat diseases is to produce cultivars resistant to their pathogens. For this purpose, old landraces are often used as genetically diverse sources of traits important for breeding. In the process of wheat breeding for resistance to tan spot caused by the fungus Pyrenophora tritici-repentis (Died.) Drechs. (abbr. Ptr), selection is carried out against the dominant allele of Tsn1, the gene of sensitivity to the toxin Ptr ToxA, which induces necrosis and represents the main pathogenicity factor of Ptr controlled by the ToxA gene. The aim of the study was to characterize a set of bread wheat (Triticum aestivum L.) accessions from the VIR collection for resistance to various Ptr populations, genotype these accessions using Xfcp623 – a DNA marker of the Tsn1 gene, and identify sources of tan spot resistance.Materials and methods. Sixty-seven accessions of winter bread wheat landraces were studied. Seedling resistance to two Ptr populations was assessed using a 5-point scale adopted at VIZR. The allelic state of Tsn1 was identified by PCR.Results. Dominant alleles of Tsn1 were found for 55% of the studied accessions. Seventeen accessions were resistant or moderately resistant to two Ptr populations and an isolate from Krasnodar Territory previously used for their characterization. Nine of them had the tsn1tsn1 genotype, and 8 had Tsn1Tsn1. The accessions mainly belonged to three agroecological groups proposed by N. I. Vavilov: “steppe winter bread wheat (Banatka wheats)”, “North European forest awnless bread wheats (Sandomirka wheats)”, and “Caucasian mountain winter bread wheat”.Conclusion. The identified 17 accessions resistant to Ptr are potential breeding sources of resistance. In the studied set of accessions, no significant relationship was found between the allelic state of the Tsn1 gene in the accession and its response to the infection with pathogen populations, including isolates with the ToxA gene.
{"title":"Resistance of old winter bread wheat landraces to tan spot","authors":"N. Mironenko, N. Kovalenko, O. A. Baranova, O. Mitrofanova","doi":"10.30901/2227-8834-2023-4-205-214","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-205-214","url":null,"abstract":"Background. The most effective and environmentally safe way to combat wheat diseases is to produce cultivars resistant to their pathogens. For this purpose, old landraces are often used as genetically diverse sources of traits important for breeding. In the process of wheat breeding for resistance to tan spot caused by the fungus Pyrenophora tritici-repentis (Died.) Drechs. (abbr. Ptr), selection is carried out against the dominant allele of Tsn1, the gene of sensitivity to the toxin Ptr ToxA, which induces necrosis and represents the main pathogenicity factor of Ptr controlled by the ToxA gene. The aim of the study was to characterize a set of bread wheat (Triticum aestivum L.) accessions from the VIR collection for resistance to various Ptr populations, genotype these accessions using Xfcp623 – a DNA marker of the Tsn1 gene, and identify sources of tan spot resistance.Materials and methods. Sixty-seven accessions of winter bread wheat landraces were studied. Seedling resistance to two Ptr populations was assessed using a 5-point scale adopted at VIZR. The allelic state of Tsn1 was identified by PCR.Results. Dominant alleles of Tsn1 were found for 55% of the studied accessions. Seventeen accessions were resistant or moderately resistant to two Ptr populations and an isolate from Krasnodar Territory previously used for their characterization. Nine of them had the tsn1tsn1 genotype, and 8 had Tsn1Tsn1. The accessions mainly belonged to three agroecological groups proposed by N. I. Vavilov: “steppe winter bread wheat (Banatka wheats)”, “North European forest awnless bread wheats (Sandomirka wheats)”, and “Caucasian mountain winter bread wheat”.Conclusion. The identified 17 accessions resistant to Ptr are potential breeding sources of resistance. In the studied set of accessions, no significant relationship was found between the allelic state of the Tsn1 gene in the accession and its response to the infection with pathogen populations, including isolates with the ToxA gene.","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":"26 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139534364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-124-132
E. Ilnitskaya, M. Makarkina, E. A. Kozhevnikov, I. Gorbunov
Background. Studying wild-growing grapevines is of interest because it expands the knowledge on the genetic diversity within the gene pool and helps to search for new resistance sources. One of the main differences between wild vines and cultivars is the type of their reproductive system: wild grapes are represented by cross-pollinated dioecious vines, while cultivated ones are, with rare exceptions, hermaphrodites capable of self-pollination. A DNA marker applicable to determine the grape flower sex has been identified.Materials and methods. The VVIB23 DNA marker to the Sex gene determining the flower sex was used to study 36 genotypes of wild-growing grapes selected in 2019–2021 within the Utrish Nature Reserve near Gelendzhik and the Krasny Les State Nature Reserve on the right bank of the Kuban River. PCR technique was applied, and the results were evaluated on a Nanofor 05 genetic analyzer.Results. The studied grapevine accessions were found to be variable for the VVIB23 SSR locus: 7 types of alleles were identified. Alleles corresponding to the male flower type were present in 14 genotypes, and those corresponding to the female type in 21. A PCR fragment whose size, according to the published data, correlated with hermaphroditism was not found, which is typical for wild genotypes. The DNA marker analysis failed to determine the flower sex of one accession.Conclusion. The results of the DNA marker analysis showed that the studied wild-growing grapevine genotypes were dioecious forms
背景。对野生葡萄藤的研究很有意义，因为它能扩大对基因库遗传多样性的了解，有助于寻找新的抗性来源。野生葡萄藤与栽培品种的主要区别之一是其生殖系统的类型：野生葡萄是异花授粉的雌雄异株葡萄藤，而栽培品种除极少数例外，是能够自花授粉的雌雄同体葡萄藤。目前已经确定了一种适用于确定葡萄花性别的 DNA 标记。使用决定花朵性别的 Sex 基因 VVIB23 DNA 标记，研究了 2019-2021 年在格连吉克附近的乌特里什自然保护区和库班河右岸的克拉斯尼列斯国家自然保护区选育的 36 种野生葡萄基因型。采用了 PCR 技术，并在 Nanofor 05 基因分析仪上对结果进行了评估。所研究的葡萄品种在 VVIB23 SSR 位点上存在变异：发现了 7 种等位基因。14 个基因型存在雄花型等位基因，21 个基因型存在雌花型等位基因。根据已公布的数据，没有发现与雌雄同体相关的 PCR 片段，这是野生基因型的典型特征。DNA 标记分析未能确定一个品种的花朵性别。DNA 标记分析结果表明，所研究的野生葡萄基因型为雌雄异株型。
{"title":"Allelic diversity of the SSR locus VVIB23 linked to the flower sex in wild-growing grapevine genotypes of Krasnodar Territory","authors":"E. Ilnitskaya, M. Makarkina, E. A. Kozhevnikov, I. Gorbunov","doi":"10.30901/2227-8834-2023-4-124-132","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-124-132","url":null,"abstract":"Background. Studying wild-growing grapevines is of interest because it expands the knowledge on the genetic diversity within the gene pool and helps to search for new resistance sources. One of the main differences between wild vines and cultivars is the type of their reproductive system: wild grapes are represented by cross-pollinated dioecious vines, while cultivated ones are, with rare exceptions, hermaphrodites capable of self-pollination. A DNA marker applicable to determine the grape flower sex has been identified.Materials and methods. The VVIB23 DNA marker to the Sex gene determining the flower sex was used to study 36 genotypes of wild-growing grapes selected in 2019–2021 within the Utrish Nature Reserve near Gelendzhik and the Krasny Les State Nature Reserve on the right bank of the Kuban River. PCR technique was applied, and the results were evaluated on a Nanofor 05 genetic analyzer.Results. The studied grapevine accessions were found to be variable for the VVIB23 SSR locus: 7 types of alleles were identified. Alleles corresponding to the male flower type were present in 14 genotypes, and those corresponding to the female type in 21. A PCR fragment whose size, according to the published data, correlated with hermaphroditism was not found, which is typical for wild genotypes. The DNA marker analysis failed to determine the flower sex of one accession.Conclusion. The results of the DNA marker analysis showed that the studied wild-growing grapevine genotypes were dioecious forms","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":"17 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139534187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-215-221
E. Radchenko, R. A. Abdullaev, K. D. Dyatlova, D. E. Akimova, I. A. Zveinek
Background. The use of barley cultivars resistant to greenbug (Schizaphis graminum Rondani) is an effective, economical and ecology-friendly way to control the dangerous phytophage. The insect is able to overcome the resistance of the host plant, which necessitates a relentless search for new genes to ensure reliable protection of barley fields from the pest.Materials and methods. Resistance to the Krasnodar greenbug population was assessed in 345 barley accessions from the Asian part of Russia. Besides, resistance to S. graminum was studied in cv. ‘Post’ (carrier of the Rsg1 gene), a pure line derived from cv. ‘Onokhoisky’ (k-16626, Buryatia) heterogeneous for this trait, and landraces from Mongolia (k-3885, k-3904, and k-4080). Juvenile plants were infested with aphids in the laboratory, and when a susceptible control died, the damage to experimental barley accessions was assessed using a scoring scale. Genetic control of greenbug resistance in the line derived from cv. ‘Onokhoisky’ was studied using the insect’s test clones and an analysis of the segregation in F2 hybrids from crossing the resistant line with cv. ‘Belogorsky’ susceptible to the aphid, when the plants were infested with the Krasnodar population of the phytophage and with clones of S. graminum.Results and conclusions. We identified 7 accessions heterogeneous for aphid resistance. Among them, plants with a high level of greenbug resistance were found in three landraces from Tuva (k-14714, k-14718, and k-14733). Resistance was also clearly expressed in the line derived from cv. ‘Onokhoisky’. This cultivar is protected by a dominant allele that differs from the previously identified Rsg1, as well as from the alleles found in Mongolian landraces k-3904, k-4080, and k-3885. ‘Onokhoisky’ also has genes with low expressivity, which manifest themselves when the main gene for resistance to S. graminum appears ineffective.
{"title":"Diversity of barley accessions from the Asian part of Russia in greenbug resistance","authors":"E. Radchenko, R. A. Abdullaev, K. D. Dyatlova, D. E. Akimova, I. A. Zveinek","doi":"10.30901/2227-8834-2023-4-215-221","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-215-221","url":null,"abstract":"Background. The use of barley cultivars resistant to greenbug (Schizaphis graminum Rondani) is an effective, economical and ecology-friendly way to control the dangerous phytophage. The insect is able to overcome the resistance of the host plant, which necessitates a relentless search for new genes to ensure reliable protection of barley fields from the pest.Materials and methods. Resistance to the Krasnodar greenbug population was assessed in 345 barley accessions from the Asian part of Russia. Besides, resistance to S. graminum was studied in cv. ‘Post’ (carrier of the Rsg1 gene), a pure line derived from cv. ‘Onokhoisky’ (k-16626, Buryatia) heterogeneous for this trait, and landraces from Mongolia (k-3885, k-3904, and k-4080). Juvenile plants were infested with aphids in the laboratory, and when a susceptible control died, the damage to experimental barley accessions was assessed using a scoring scale. Genetic control of greenbug resistance in the line derived from cv. ‘Onokhoisky’ was studied using the insect’s test clones and an analysis of the segregation in F2 hybrids from crossing the resistant line with cv. ‘Belogorsky’ susceptible to the aphid, when the plants were infested with the Krasnodar population of the phytophage and with clones of S. graminum.Results and conclusions. We identified 7 accessions heterogeneous for aphid resistance. Among them, plants with a high level of greenbug resistance were found in three landraces from Tuva (k-14714, k-14718, and k-14733). Resistance was also clearly expressed in the line derived from cv. ‘Onokhoisky’. This cultivar is protected by a dominant allele that differs from the previously identified Rsg1, as well as from the alleles found in Mongolian landraces k-3904, k-4080, and k-3885. ‘Onokhoisky’ also has genes with low expressivity, which manifest themselves when the main gene for resistance to S. graminum appears ineffective.","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-133-142
A. Lyzhin, N. Saveleva
Background. Fire blight (Erwinia amylovora (Burill) Winslow et al.) is a dangerous disease of fruit crops. In the Russian Federation, fire blight is one of the quarantine diseases. Apple resistance to fire blight is controlled polygenically. However, the data on the genotypes of Malus Mill. wild species and varieties in the context of individual resistance loci are scarce. The purpose of the research was studying the genetic collection of apple wild species according to the FBF7 fire blight resistance locus to identify promising sources of resistance to E. amylovora.Materials and methods. The materials of this study included 23 wild and two cultivated apple-tree species (50 accessions) from the genetic collection of the I.V. Michurin FSC. The FBF7 QTL for fire blight resistance was identified with the flanking SCAR-markers AE10-375 and GE-8019, and SSR-marker CH-F7-Fb1.Results and conclusion. The GE-8019 marker was identified in 50.0% of the apple accessions, AE10-375 in 76.0% of the accessions, and CH-F7-Fb1 in 30.0%. Inter- and intraspecific polymorphism of the Malus genus was revealed for the analyzed resistance locus, as well as for individual DNA markers linked to the FBF7 QTL. The GE-8019 and AE10-375 markers flanking the FBF7 QTL were found in 42.0% of the studied genotypes. Meanwhile, three diagnostic markers (GE-8019, AE10-375, and CH-F7-Fb1) were identified only in 7 accessions: M. × robusta var. persicifolia (Carr.) Rehd. (к-41279), M. × cerasifera var. hiemalis Spach. (к-2342) (Ser. Baccatae), M. sylvestris (L.) Mill. (k-73 and k-123), M. × spectabilis var. albi plena (Ait.) Borkh. (к-2416) (Ser. Malus), M. × sargentii (Rehd.) Langenf. (k-2428) (Ser. Toringonae), and M. coronaria (L.) Mill. (к-2336) (Ser. Coronariae). These forms are promising genetic sources of resistance to E. amylovora.
背景。火疫病（Erwinia amylovora (Burill) Winslow et al.）是水果作物的一种危险病害。在俄罗斯联邦，火疫病是检疫病害之一。苹果对火萎病的抗性是由多基因控制的。然而，有关 Malus Mill. 野生种和品种在单个抗性基因座方面的基因型数据却很少。本研究的目的是根据 FBF7 火疫病抗性基因座研究苹果野生种的基因收集，以确定对 E. amylovora 有希望的抗性来源。本研究的材料包括来自 I.V. Michurin FSC 基因库的 23 个野生苹果树品种和 2 个栽培苹果树品种（50 个登录品系）。通过侧翼 SCAR 标记 AE10-375 和 GE-8019，以及 SSR 标记 CH-F7-Fb1，鉴定了 FBF7 抗枯萎病 QTL。50.0%的苹果品种鉴定出了 GE-8019 标记，76.0%的品种鉴定出了 AE10-375，30.0%的品种鉴定出了 CH-F7-Fb1。所分析的抗性基因座以及与 FBF7 QTL 相连的单个 DNA 标记均显示出马铃薯属的种间和种内多态性。在 42.0% 的研究基因型中发现了位于 FBF7 QTL 外侧的 GE-8019 和 AE10-375 标记。同时，三个诊断标记（GE-8019、AE10-375 和 CH-F7-Fb1）仅在 7 个基因型中发现：(к-41279), M. × cerasifera var.(к-2342) (Ser. Baccatae), M. sylvestris (L.) Mill.(k-73 and k-123), M. × spectabilis var.(к-2336) (Ser. Coronariae)。这些形态是抗 E. amylovora 的有希望的基因来源。
{"title":"Occurrence of the FBF7 fire blight resistance locus in accessions of apple wild species (Malus Mill.)","authors":"A. Lyzhin, N. Saveleva","doi":"10.30901/2227-8834-2023-4-133-142","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-133-142","url":null,"abstract":"Background. Fire blight (Erwinia amylovora (Burill) Winslow et al.) is a dangerous disease of fruit crops. In the Russian Federation, fire blight is one of the quarantine diseases. Apple resistance to fire blight is controlled polygenically. However, the data on the genotypes of Malus Mill. wild species and varieties in the context of individual resistance loci are scarce. The purpose of the research was studying the genetic collection of apple wild species according to the FBF7 fire blight resistance locus to identify promising sources of resistance to E. amylovora.Materials and methods. The materials of this study included 23 wild and two cultivated apple-tree species (50 accessions) from the genetic collection of the I.V. Michurin FSC. The FBF7 QTL for fire blight resistance was identified with the flanking SCAR-markers AE10-375 and GE-8019, and SSR-marker CH-F7-Fb1.Results and conclusion. The GE-8019 marker was identified in 50.0% of the apple accessions, AE10-375 in 76.0% of the accessions, and CH-F7-Fb1 in 30.0%. Inter- and intraspecific polymorphism of the Malus genus was revealed for the analyzed resistance locus, as well as for individual DNA markers linked to the FBF7 QTL. The GE-8019 and AE10-375 markers flanking the FBF7 QTL were found in 42.0% of the studied genotypes. Meanwhile, three diagnostic markers (GE-8019, AE10-375, and CH-F7-Fb1) were identified only in 7 accessions: M. × robusta var. persicifolia (Carr.) Rehd. (к-41279), M. × cerasifera var. hiemalis Spach. (к-2342) (Ser. Baccatae), M. sylvestris (L.) Mill. (k-73 and k-123), M. × spectabilis var. albi plena (Ait.) Borkh. (к-2416) (Ser. Malus), M. × sargentii (Rehd.) Langenf. (k-2428) (Ser. Toringonae), and M. coronaria (L.) Mill. (к-2336) (Ser. Coronariae). These forms are promising genetic sources of resistance to E. amylovora.","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":"39 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-163-173
N. A. Oskina, T. A. Gavrilenko, I. Chukhina
Taxonomic studies were carried out in the collection of cultivated potato species kept in the Herbarium of Cultivated Plants of the World, Their Wild Relatives, and Weeds (WIR). It allowed us to proceed with typifying the names of intraspecific taxa within Solanum andigenum Juz. et Buk., belonging to three geographical groups: Colombian, South Peruvian, and Bolivian. The WIR herbarium collections comprise the taxonomic diversity of cultivated potatoes described by Russian taxonomists and based on the study of plant materials collected in South America during the expeditions organized by N. I. Vavilov in the late 1920s – early 1930s. Verification of protologs and herbarium specimens resulted in locating herbarium sheets that appeared to be syntypes of six varieties and eleven forms. Lectotypes were also designated for the following three forms: S. andigenum f. huaman-uma Buk. et Lech., S. andigenum f. lecke-umo Buk. et Lech., S. andigenum f. pacus Lech.
对世界栽培植物、野生近缘植物和杂草标本馆（WIR）收藏的栽培马铃薯物种进行了分类研究。通过这项研究，我们对 Solanum andigenum Juz：哥伦比亚、南秘鲁和玻利维亚。世界马铃薯研究所标本馆藏品包括俄罗斯分类学家描述的栽培马铃薯的分类多样性，这些分类多样性是基于对 19 世纪末 N. I. Vavilov 组织的考察期间在南美洲收集的植物材料的研究。Vavilov 在 20 世纪 20 年代末至 30 年代初组织的考察中在南美洲收集的植物材料。通过对原生植物和标本馆标本的核实，找到了标本馆标本，这些标本似乎是 6 个品种和 11 个形态的同种异型。还为以下三种形式指定了分型：S. andigenum f. huaman-uma Buk. et Lech.、S. andigenum f. lecke-umo Buk. et Lech.、S. andigenum f. pacus Lech.
{"title":"Typification of intraspecific taxa in Solanum andigenum Juz. et Buk.","authors":"N. A. Oskina, T. A. Gavrilenko, I. Chukhina","doi":"10.30901/2227-8834-2023-4-163-173","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-163-173","url":null,"abstract":"Taxonomic studies were carried out in the collection of cultivated potato species kept in the Herbarium of Cultivated Plants of the World, Their Wild Relatives, and Weeds (WIR). It allowed us to proceed with typifying the names of intraspecific taxa within Solanum andigenum Juz. et Buk., belonging to three geographical groups: Colombian, South Peruvian, and Bolivian. The WIR herbarium collections comprise the taxonomic diversity of cultivated potatoes described by Russian taxonomists and based on the study of plant materials collected in South America during the expeditions organized by N. I. Vavilov in the late 1920s – early 1930s. Verification of protologs and herbarium specimens resulted in locating herbarium sheets that appeared to be syntypes of six varieties and eleven forms. Lectotypes were also designated for the following three forms: S. andigenum f. huaman-uma Buk. et Lech., S. andigenum f. lecke-umo Buk. et Lech., S. andigenum f. pacus Lech.","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":"58 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-251-261
A. Editorial
.
.
{"title":"Supplements to the Proceedings on Applied Botany, Genetics and Breeding: dedicated to the 115th anniversary of the journal","authors":"A. Editorial","doi":"10.30901/2227-8834-2023-4-251-261","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-251-261","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":"50 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-143-152
M. V. Gurkina, M. Burlyaeva
Background. Cowpea is an important legume crop of great nutritional and economic value, ranking fourth in the world among leguminous crops in the area of cultivation. This crop is known to tolerate high temperatures and droughts, so new cowpea cultivars adapted to the local arid conditions of the Lower Volga are of considerable interest.Materials and methods. Mass and individual selection techniques were applied to develop new cultivars from cowpea landraces held in the VIR collection. Selection was carried out at Astrakhan Experiment Station of VIR in 2012–2018, and variety trials took place in 2019–2021.Results. Cvs. ‘Samma Nova’ and ‘Pasta Grin’ were released for vegetable use. The pod yield of ‘Samma Nova’ is 12.18–14.85 t/ha, seed yield is 0.92–1.14 t/ha, protein content is 28.8% in seeds and 27.1% in pods, and its growing season is 57–66 days. The cultivar is bushy, with synchronous pod and seed maturation, suitable for mechanized cultivation. The pod yield of ‘Pasta Grin’ is 24.68–25.84 t/ha, seed yield is 0.82–1.04 t/ha, protein content is 29.1% in pods and 23.6% in seeds. It is a mid-season cultivar, with a rambling stem, intended for growing on a trellis. Both cultivars significantly exceeded the reference (cv. ‘Sibirsky Razmer”) in most of the studied indicators. The breeding work with cowpea accessions showed that pod harvesting at the commercial ripeness stage stimulated plants to develop new pods, which extended the duration of fruiting and increased productivity. The maximum number of pods in all studied cultivars was formed in the first 20 days of the fruiting period.Conclusion. In 2022, according to the results of competitive and local trials, cvs. ‘Samma Nova’ and ‘Pasta Grin’ were included in the State Register for Selection Achievements (National List). The zones of cultivation and seed production for these cultivars are Astrakhan and Volgograd Provinces, Krasnodar and Stavropol Territories, and the Republic of Crimea.
{"title":"New cultivars of vegetable cowpea (Vigna unguiculata (l.) walp.) developed at VIR","authors":"M. V. Gurkina, M. Burlyaeva","doi":"10.30901/2227-8834-2023-4-143-152","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-143-152","url":null,"abstract":"Background. Cowpea is an important legume crop of great nutritional and economic value, ranking fourth in the world among leguminous crops in the area of cultivation. This crop is known to tolerate high temperatures and droughts, so new cowpea cultivars adapted to the local arid conditions of the Lower Volga are of considerable interest.Materials and methods. Mass and individual selection techniques were applied to develop new cultivars from cowpea landraces held in the VIR collection. Selection was carried out at Astrakhan Experiment Station of VIR in 2012–2018, and variety trials took place in 2019–2021.Results. Cvs. ‘Samma Nova’ and ‘Pasta Grin’ were released for vegetable use. The pod yield of ‘Samma Nova’ is 12.18–14.85 t/ha, seed yield is 0.92–1.14 t/ha, protein content is 28.8% in seeds and 27.1% in pods, and its growing season is 57–66 days. The cultivar is bushy, with synchronous pod and seed maturation, suitable for mechanized cultivation. The pod yield of ‘Pasta Grin’ is 24.68–25.84 t/ha, seed yield is 0.82–1.04 t/ha, protein content is 29.1% in pods and 23.6% in seeds. It is a mid-season cultivar, with a rambling stem, intended for growing on a trellis. Both cultivars significantly exceeded the reference (cv. ‘Sibirsky Razmer”) in most of the studied indicators. The breeding work with cowpea accessions showed that pod harvesting at the commercial ripeness stage stimulated plants to develop new pods, which extended the duration of fruiting and increased productivity. The maximum number of pods in all studied cultivars was formed in the first 20 days of the fruiting period.Conclusion. In 2022, according to the results of competitive and local trials, cvs. ‘Samma Nova’ and ‘Pasta Grin’ were included in the State Register for Selection Achievements (National List). The zones of cultivation and seed production for these cultivars are Astrakhan and Volgograd Provinces, Krasnodar and Stavropol Territories, and the Republic of Crimea.","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":" 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139625404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.30901/2227-8834-2023-4-190-204
E. Gultyaeva, E. Shaydayuk, R. E. Smirnova, K. M. Abdullaev, K. U. Kurkiev
Background. Yellow rust incidence on Triticum L. has been observed to surge up in Dagestan in recent years, facilitating selection of highly resistant genotypes. Information on the effectiveness of Yr genes and the pathogen’s virulence and racial composition is needed to interpret field assessment data correctly. The objective of this study was to describe the virulence of the Dagestan population of Puccinia striiformis West. at the seedling stage, evaluate the resistance of differentiators to yellow rust in the field, and identify invasive PstS1 and PstS2 races.Materials and methods. Leaves with urediniopustules were collected from bread wheat accessions at Dagestan Experiment Station of VIR (DЕS VIR) in 2020–2022. Virulence was analyzed in the laboratory using 14 AvYr lines (Avocet NIL) and 15 differential wheat cultivars. Age-specific resistance of virulence testers under high natural infection pressure was studied in the field of DES VIR. A set of SCAR markers (SCP19M24a1, SCP19M24a2, SCP19M26a1, and SCP19M26a2) were used to identify invasive races.Results and discussion. Avocet lines with Yr5, Yr10, Yr15, Yr24, and Yr26, and cv. ‘Moro’ (Yr10, YrMor) manifested resistance to all isolates. Virulence to Yr17 was detected only in the isolates from cvs. ‘Graf’ and ‘Svarog’ carrying this gene, and ‘Siete Ceros’. The Dagestan collection demonstrated high genetic diversity. Common phenotypes were identified on cv. ‘Graf’ in 2021 and 2020. There was no temporal differentiation between pathogen collections in the years of testing. High field resistance to yellow rust was recorded in lines with Yr5, Yr8, Yr10, Yr15 and Yr24, and in cvs. ‘Moro’, ‘Compair’, ‘Carstens’ and ‘Spaldings Prolific’. Cvs. ‘Reichersberg 42’ and ‘Heines Peko Vilmorin 23’ were resistant in 2020 and 2022, and moderately affected (up to 10%) in 2021. Molecular analysis identified all three isolates belonging to the invasive PstS2 group on cv. ‘Siete Cerros’ and L 650 in 2021, and cv. ‘Vassa’ in 2022.
{"title":"Virulence diversity of the yellow rust pathogen population in Dagestan","authors":"E. Gultyaeva, E. Shaydayuk, R. E. Smirnova, K. M. Abdullaev, K. U. Kurkiev","doi":"10.30901/2227-8834-2023-4-190-204","DOIUrl":"https://doi.org/10.30901/2227-8834-2023-4-190-204","url":null,"abstract":"Background. Yellow rust incidence on Triticum L. has been observed to surge up in Dagestan in recent years, facilitating selection of highly resistant genotypes. Information on the effectiveness of Yr genes and the pathogen’s virulence and racial composition is needed to interpret field assessment data correctly. The objective of this study was to describe the virulence of the Dagestan population of Puccinia striiformis West. at the seedling stage, evaluate the resistance of differentiators to yellow rust in the field, and identify invasive PstS1 and PstS2 races.Materials and methods. Leaves with urediniopustules were collected from bread wheat accessions at Dagestan Experiment Station of VIR (DЕS VIR) in 2020–2022. Virulence was analyzed in the laboratory using 14 AvYr lines (Avocet NIL) and 15 differential wheat cultivars. Age-specific resistance of virulence testers under high natural infection pressure was studied in the field of DES VIR. A set of SCAR markers (SCP19M24a1, SCP19M24a2, SCP19M26a1, and SCP19M26a2) were used to identify invasive races.Results and discussion. Avocet lines with Yr5, Yr10, Yr15, Yr24, and Yr26, and cv. ‘Moro’ (Yr10, YrMor) manifested resistance to all isolates. Virulence to Yr17 was detected only in the isolates from cvs. ‘Graf’ and ‘Svarog’ carrying this gene, and ‘Siete Ceros’. The Dagestan collection demonstrated high genetic diversity. Common phenotypes were identified on cv. ‘Graf’ in 2021 and 2020. There was no temporal differentiation between pathogen collections in the years of testing. High field resistance to yellow rust was recorded in lines with Yr5, Yr8, Yr10, Yr15 and Yr24, and in cvs. ‘Moro’, ‘Compair’, ‘Carstens’ and ‘Spaldings Prolific’. Cvs. ‘Reichersberg 42’ and ‘Heines Peko Vilmorin 23’ were resistant in 2020 and 2022, and moderately affected (up to 10%) in 2021. Molecular analysis identified all three isolates belonging to the invasive PstS2 group on cv. ‘Siete Cerros’ and L 650 in 2021, and cv. ‘Vassa’ in 2022.","PeriodicalId":36512,"journal":{"name":"Proceedings on Applied Botany, Genetics and Breeding","volume":" 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139625774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：