Aquaculture and Fisheries最新文献_第8页

Polymorphisms in cathepsin b is associated with growth in Asian seabass 组织蛋白酶b的多态性与亚洲鲈鱼的生长有关

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-06-18 DOI: 10.1016/j.aaf.2025.05.003

Shadame Yeo , Joey Wong , Y.X. Tay , Zituo Yang , Le Wang , Fei Sun , May Lee , Yanfei Wen , Gen Hua Yue

This study investigates the relationship between polymorphisms in the cathepsin B (ctsb) gene and body weight in Asian seabass (Lates calcarifer). Despite the economic significance of this species, the genetic factors that influence growth performance in fish remain poorly understood. CTSB, a lysosomal cysteine protease involved in protein degradation and tissue remodelling, is a crucial regulator of growth. We analysed the full-length cDNA of ctsb and mapped it to the major quantitative trait locus (QTL) for growth on linkage group 2 in L. calcarifer. Single nucleotide polymorphisms (SNPs) within ctsb were identified and their associations with body weight were analysed in 298 individuals. Results revealed that SNP1 (C > T) in intron 2 of ctsb was significantly correlated with body weight. Quantitative real-time PCR (qRT-PCR) showed that ctsb was expressed in all tissues, with the highest expression in the gills and kidney of developing seabass. Knockdown/overexpression of ctsb in cell lines respectively promoted/inhibited cell proliferation without altering cell size. These findings suggest that ctsb plays a significant role in growth regulation in Asian seabass. This study provides a potential molecular marker for selecting fast-growing Asian seabass. Future research should focus on identifying causal variants in ctsb associated with accelerated growth, conducting in vivo studies, and exploring related signalling pathways.

本研究探讨了亚洲海鲈组织蛋白酶B （ctsb）基因多态性与体重的关系。尽管这一物种具有重要的经济意义，但影响鱼类生长性能的遗传因素仍然知之甚少。CTSB是一种参与蛋白质降解和组织重塑的溶酶体半胱氨酸蛋白酶，是一种重要的生长调节剂。我们分析了ctsb的全长cDNA，并将其定位到钙化L.连锁群2上生长的主要数量性状位点（QTL）。在298例个体中鉴定了ctsb内的单核苷酸多态性（snp），并分析了其与体重的关系。结果显示，ctsb内含子2中的SNP1 （C >； T）与体重显著相关。实时荧光定量PCR （Quantitative real-time PCR, qRT-PCR）结果显示，ctsb在发育中的鲈鱼各组织中均有表达，其中鳃和肾表达量最高。ctsb在细胞系中敲低/过表达分别促进/抑制细胞增殖，但不改变细胞大小。这些结果表明，ctsb在亚洲鲈鱼的生长调节中起着重要作用。该研究为快速生长的亚洲海鱼的筛选提供了潜在的分子标记。未来的研究应侧重于识别与ctsb加速生长相关的因果变异，进行体内研究，并探索相关的信号通路。

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引用次数: 0

Effects of salinities on the growth performance, muscle's flavor and transcriptomic responses in yellowfin seabream (Acanthopagrus latus) 盐度对黄鳍鱼生长性能、肌肉风味及转录组反应的影响

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-06-03 DOI: 10.1016/j.aaf.2025.05.005

Guobin Zheng , Liangmin Huang , Dongling Zhang , Jing Zhang , Shuiqing Wu , Xiande Liu

Yellowfin seabream (Acanthopagrus latus) is a euryhaline fish species of significant economic importance, and salinity, as one of important ecological factors, widely affects its growth, metabolism, and flesh quality of the fish. Current study was conducted to investigate the growth performance, muscle's flavor and transcriptomic responses of A. latus (8.25 ± 1.85 g) cultured in different salinities (6, 13, 20, 27 and 34 psμ). At the end of the 12-week culture trials, the final size, weight gain and specific growth rate (SGR) of A. latus were significantly impacted by salinity, with the highest SGR at 20 psμ (P < 0.05) and lowest at 34 psμ. The contents of polyunsaturated fatty acid (PUFA) (including eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA) and linoleic acid in at 6 psμ were significantly higher than those at any other salinity (P < 0.05). The total content of volatile compounds in flesh was the highest in 20 psμ, of which there were more abundant aldehydes and ketones. Transcriptomic analysis identified differentially expressed genes (DEGs) between the 20 psμ and 34 psμ, with 161 genes up-regulated and 86 genes down-regulated in the 20 psμ. These DEGs were associated with muscle growth and development, amino acid metabolism, carbohydrate metabolism, and others. The reliability of transcriptome data was validated using qRT-PCR based on ten selected growth-related genes, which showed the same trends as the RNA-seq results. From the overall results of this study, we infer the growth performance and flavor can be significantly improved at the salinity of 20 psμ, and the fatty acid composition can be significantly improved at the salinity of 6 psμ for yellowfin seabream.

黄鳍海鲷（Acanthopagrus latus）是一种具有重要经济价值的泛盐鱼类，盐度作为重要的生态因子之一，广泛影响着黄鳍海鲷的生长、代谢和肉质。本研究研究了在不同盐度（6、13、20、27和34 psμ）下培养的鱼（8.25±1.85 g）的生长性能、肌肉风味和转录组反应。在12周的培养试验结束时，盐度对鱼的最终大小、增重和特定生长率（SGR）有显著影响，在20 psμ时SGR最高（P < 0.05），在34 psμ时SGR最低。多不饱和脂肪酸（PUFA）（包括二十碳五烯酸、EPA和二十二碳六烯酸、DHA）和亚油酸含量显著高于其他盐度（P < 0.05）。果肉中挥发性物质的总含量在20 pμ左右最高，其中醛类和酮类含量较高。转录组学分析发现20和34个psμ之间存在差异表达基因（DEGs），其中20个psμ中有161个基因上调，86个基因下调。这些deg与肌肉生长发育、氨基酸代谢、碳水化合物代谢等有关。基于10个选择的生长相关基因，使用qRT-PCR验证了转录组数据的可靠性，结果显示与RNA-seq结果相同的趋势。从本研究的总体结果来看，盐度为20 psμ可显著改善黄鳍鲷的生长性能和风味，盐度为6 psμ可显著改善黄鳍鲷的脂肪酸组成。

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引用次数: 0

Isolation and identification of the pathogen in a case of Takifugu rubripes coinfected with parasites and bacteria 1例红鳍东方鲀寄生虫与细菌共感染病原菌的分离鉴定

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-06-02 DOI: 10.1016/j.aaf.2025.04.003

Gengtong Zhao , Xinpeng Ni , Ziyi Zhang , Meili Pan , Xiaohang Wang , Yuyu Xiong , Xiuli Wang , Ruijun Li

In August 2023, a significant outbreak occurred at a puffer fish farm in Dandong, Liaoning, China, affecting a large number of Takifugu rubripes. The affected fish displayed symptoms including fin ulcers and white spots, which were confirmed through clinical testing conducted at Dalian Ocean University. Microscopic examinations revealed a substantial presence of black dot-like unicellular parasites on the gills of the diseased fish. Further analyses, including methyl green-pyronine staining, optical microscopy, and biological scanning electron microscopy, facilitated the identification of the parasite as Amyloodinium ocellatum. Additionally, bacterial isolation and culture, Gram staining, physiological and biochemical testing, 16S rDNA sequence analysis, phylogenetic tree, and drug sensitivity experiments were performed on the organs of the affected fish. The results identified two dominant bacterial strains, Vibrio scophthalmi and Vibrio harveyi, present in the spleen and kidney of the infected fish. Antibiotic sensitivity tests indicated that V. scophthalmi is susceptible to antibiotics such as ciprofloxacin, florfenicol, and enrofloxacin, while V. harveyi showed sensitivity to ciprofloxacin, florfenicol, and ofloxacin. In conclusion, this case was determined to be a combined infection involving both parasites and bacteria. The findings of this study offer valuable insights for the prevention and control of epidemics affecting Takifugu rubripes.

2023年8月，中国辽宁省丹东市一个河豚养殖场发生重大疫情，影响大量红鳍东方鲀。受感染的鱼表现出鳍溃疡和白斑等症状，经大连海洋大学的临床检测证实。显微镜检查显示病鱼的鳃上有大量黑点样的单细胞寄生虫。进一步的分析，包括甲基绿吡啶染色，光学显微镜和生物扫描电子显微镜，有助于确定寄生虫为淀粉样细胞。对病鱼器官进行细菌分离培养、革兰氏染色、生理生化检测、16S rDNA序列分析、系统发育树和药敏实验。结果鉴定出两种优势菌株，镰刀弧菌和哈维弧菌，存在于感染鱼的脾脏和肾脏中。抗生素敏感性试验表明，沙眼弧菌对环丙沙星、氟苯尼考、恩诺沙星等抗生素敏感，而哈维弧菌对环丙沙星、氟苯尼考、氧氟沙星等抗生素敏感。综上所述，本病例为寄生虫和细菌联合感染。本研究结果为预防和控制影响红鳍东方鲀的流行病提供了有价值的见解。

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引用次数: 0

Evaluating the genetic diversity in farmed populations of Nile tilapia (Oreochromis niloticus) from Brazil using SNP markers 利用SNP标记评价巴西尼罗罗非鱼养殖种群的遗传多样性

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-05-31 DOI: 10.1016/j.aaf.2025.05.004

Vito Antonio Mastrochirico-Filho , Baltasar Fernandes Garcia , Lieschen Valeria Guerra Lira , Antonio Ramon do Amaral Neto , Rodrigo Marín-Nahuelpi , Jousepth Gallardo-Hidalgo , Liane Ney Bassini , Fabio Porto-Foresti , Diogo Teruo Hashimoto

The Nile tilapia (Oreochromis niloticus) plays a significant role in global aquaculture, with Brazil ranking among the top producers worldwide. However, a considerable gap remains in the exploration of genetic diversity within Brazilian Nile tilapia stocks. To address this, we analyzed the genetic diversity of nine distinct farmed populations of Nile tilapia in Brazil, comprising a total of 600 individuals, using single-nucleotide polymorphisms (SNPs). Our objective was to provide essential genetic insights to establish the foundation for a new breeding nucleus. The pre-breeding populations exhibited high genetic diversity. Among them, Pop1 had the lowest diversity, characterized by the smallest proportion of polymorphic SNPs (N_P = 92.3%), the lowest mean minor allele frequency (MAF = 0.24), and the lowest heterozygosity (H_E = 0.32). In contrast, Pop5 and Pop9 displayed the highest genetic diversity (MAF = 0.30; H_E = H_O 0.39), with nearly all loci in Hardy-Weinberg equilibrium (HWE = 99.4%). Additionally, reduced effective population sizes (N_e) were observed in Pop1 (N_e = 6.8) and Pop4 (N_e = 15.4), with approximately 40 % of individuals classified as half-of full siblings, suggesting risks of inbreeding. These characteristics led to the genetic structuring of Pop1 and Pop4 in relation to the other populations, while Pop5 and Pop9 also formed a distinct cluster due to their higher genetic diversity. Despite the overall high genetic diversity observed across the analyzed populations, some populations exhibited parameters that may indicate potential future issues related to genetic diversity loss if not properly managed. Therefore, the findings presented here will help ensure the implementation of effective strategies during the pre-breeding phase of a new tilapia genetic improvement nucleus, supporting the long-term maintenance of genetic diversity in the breeding stock.

尼罗罗非鱼（Oreochromis niloticus）在全球水产养殖中发挥着重要作用，巴西是世界上最大的罗非鱼生产国之一。然而，在探索巴西尼罗罗非鱼种群的遗传多样性方面仍存在相当大的差距。为了解决这个问题，我们利用单核苷酸多态性（snp）分析了巴西9个不同的尼罗罗非鱼养殖种群的遗传多样性，共包括600个个体。我们的目标是提供必要的遗传见解，以建立一个新的育种核的基础。育种前群体表现出较高的遗传多样性。其中，Pop1的多样性最低，多态性snp比例最小（NP = 92.3%），平均次要等位基因频率最低（MAF = 0.24），杂合性最低（HE = 0.32）。相比之下，Pop5和Pop9的遗传多样性最高（MAF = 0.30, HE = 0.39），几乎所有位点都处于Hardy-Weinberg平衡（HWE = 99.4%）。此外，在Pop1 （Ne = 6.8）和Pop4 （Ne = 15.4）中观察到有效种群大小（Ne）减小，大约40%的个体被归类为半全兄弟姐妹，提示近亲繁殖的风险。这些特征导致Pop1和Pop4的遗传结构相对于其他居群，而Pop5和Pop9也因其较高的遗传多样性而形成了一个明显的集群。尽管在分析的种群中观察到总体上较高的遗传多样性，但一些种群表现出的参数可能表明，如果管理不当，未来可能会出现与遗传多样性丧失有关的潜在问题。因此，本文提出的研究结果将有助于确保在新的罗非鱼遗传改良核的育种前阶段实施有效的策略，支持长期维持种畜的遗传多样性。

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引用次数: 0

Genetic diversity and population structure of different red swamp crayfish (Procambarus clarkii) populations across the north and south of China 中国南北不同沼泽红螯虾（原螯虾）种群的遗传多样性和种群结构

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-05-31 DOI: 10.1016/j.aaf.2025.04.004

Qing Mu , Chuntong Liu , Zhiyan Wang , Qizhou Xu , Liang Jia , Zhiyi Bai

The red swamp crayfish, Procambarus clarkii, has become one of the most important freshwater aquaculture species in China. The crayfish culture extends from the middle and lower areas of Yangtze River to the northern and southern part of China in recent ten years. To understand genetic variability during geographical adaption, we performed whole genome re-sequencing (WGS) of six crayfish populations across the north and south of China: Panjin (PJ), Liaoning province; Weishan (WS), Shandong province; Wuhu (WH), Anhui province; Jianhu (JH), Jiangsu province; Gehu (GH), Jiangsu province; Haikou (HK), Hainan province. A total of 120,906,91 high-quality single nucleotide polymorphisms (SNPs) were identified across the crayfish genome. The six geographical populations showed high level of diversity with the expected heterozygosity (He) ranged from 0.291 (WS) to 0.314 (PJ). The principal component and neighbor-joining (NJ) phylogeny analysis categorized the 180 individuals into three subgroups: the PJ and WS populations formed two separate subgroups, while the GH, JH, WH, and HK populations converged into a single subgroup. Moreover, the pairwise comparison of fixation index (Fst) values indicated a minor to moderate level of genetic differentiation among these populations, ranging from 0.0037 (GH, JH, WH & HK) to 0.0717 (WS & PJ), suggesting a greater genetic distance between the WS population and PJ population. Taken together, the SNPs identified in this study can serve as valuable genetic markers in future germplasm improvement of P. clarkii cultured stocks. These results will provide genetic information for further germplasm conservation and genetic improvement of P. clarkii.

克氏原螯虾（Procambarus clarkii）已成为中国最重要的淡水养殖品种之一。近十年来，小龙虾养殖从长江中下游地区向中国北部和南部地区扩展。为了了解地理适应过程中的遗传变异，我们对中国北部和南部的6个小龙虾种群进行了全基因组重测序（WGS）：辽宁盘锦；山东微山（WS）；安徽芜湖；江苏建湖；江苏葛湖（GH）；海口（香港），海南省。在整个小龙虾基因组中共鉴定出120,906,91个高质量的单核苷酸多态性（snp）。6个地理居群的期望杂合度（He）在0.291 (WS) ~ 0.314 （PJ）之间，具有较高的多样性。主成分和邻联（NJ）系统发育分析将180个个体划分为3个亚群：PJ和WS居群为两个独立的亚群，GH、JH、WH和HK居群为一个亚群。此外，固定指数（Fst）两两比较表明，这些群体之间的遗传分化程度为轻微至中等，范围为0.0037 （GH， JH, WH & HK）至0.0717 (WS &； PJ)，表明WS与PJ群体之间存在较大的遗传距离。综上所述，本研究所鉴定的snp可作为未来克氏假单胞菌种质改良中有价值的遗传标记。这些结果将为进一步的克氏假单胞菌种质资源保护和遗传改良提供遗传信息。

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引用次数: 0

Combined supplementation of hydroxyproline and vitamin C improved the growth and flesh quality of Pacific white shrimp (Litopenaeus vanname) cultured in low salinity water 联合补充羟脯氨酸和维生素 C 可改善低盐度水域养殖的太平洋南美白对虾的生长和肉质

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2023.12.007

Tianyu Huang , Beibei Guo , Jinyu Zheng , Menglu Li , Yunfeng Chen , Xiaoqin Li , Xiangjun Leng

This study investigated the effects of hydroxyproline (Hyp) and vitamin C (VC) addition to practical diet on growth and flesh quality of Pacific white shrimp (Litopenaeus vannamei) cultured in low salinity water. The control diet was formulated to contain 200 g/kg fish meal (FM20), and then 14 g/kg coated hydroxyproline (50%) (FM20H), 2 g/kg L-ascorbyl-2-phosphate (38%) (FM20C) and their combination (FM20HC) were added to the control diet to form four isoproteic (41.6%) and isolipidic (6.7%) diets. Pacific white shrimp with initial body weight of (1.50 ± 0.05) g were fed the above four diets in low salinity water (salinity 1‰) for 8 weeks. The results showed that the combined supplementation of Hyp and VC (FM20HC) increased the weight gain by 13.4%, and decreased the feed conversion ratio by 0.18 (P < 0.05), while the individual supplementation (FM20H and FM20C groups) just numerically promoted the growth (P > 0.05). In addition, the FM20HC group also showed higher protein and lipid retention than the FM20 group (P < 0.05). In flesh quality, the supplementation of Hyp (FM20H) increased total collagen and heat-soluble collagen contents, flesh chewiness and free flavor amino acids contents (P < 0.05). The supplementation of VC (FM20C) increased the contents of total collagen, heat-insoluble collagen and free flavor amino acids, and decreased flesh lactic acid, malondialdehyde contents and steaming loss (P < 0.05). The combined supplementation of Hyp and VC (FM20HC) showed higher total collagen and heat-insoluble collagen contents than the individual supplementation (FM20H and FM20C) (P < 0.05), and the myofiber density, flesh hardness and shear force in the FM20HC group were also significantly higher than those in the control (P < 0.05). In summary, the combined supplementation of hydroxyproline and VC in practical diet with fishmeal content of 200 g/kg significantly improved the growth performance and flesh quality of L. vannamei cultured in low salinity water.

本试验研究了实用饲料中添加羟脯氨酸（Hyp）和维生素C （VC）对凡纳滨对虾（Litopenaeus vannamei）生长和肉质的影响。在对照饲料中添加200 g/kg鱼粉（FM20），然后在对照饲料中添加14 g/kg包被羟脯氨酸（50%）（FM20H）、2 g/kg l-抗坏血酸-2-磷酸（38%）（FM20C）及其组合（FM20HC），组成4种异蛋白（41.6%）和等脂（6.7%）饲料。初始体重为（1.50±0.05）g的太平洋白对虾在低盐度（盐度为1‰）水中投喂上述4种饲料，为期8周。结果表明：饲粮中添加Hyp和VC （FM20HC）可使肉鸡增重提高13.4%，饲料系数降低0.18 (P <；0.05)，而单独添加（FM20H和FM20C组）仅在数值上促进了生长(P >；0.05)。此外，FM20HC组也比FM20组表现出更高的蛋白质和脂质潴留(P <；0.05)。肉质方面，添加Hyp （FM20H）提高了总胶原蛋白和热溶性胶原蛋白含量、肉质的咀嚼性和游离风味氨基酸含量(P <；0.05)。VC （FM20C）的添加提高了总胶原蛋白、热不溶性胶原蛋白和游离风味氨基酸的含量，降低了肉乳酸、丙二醛含量和蒸损(P <；0.05)。与单独添加FM20H和FM20C相比，Hyp和VC （FM20HC）联合添加的总胶原和热不溶性胶原含量更高(P <；0.05)， FM20HC组肌纤维密度、肌体硬度和剪切力也显著高于对照组(P <；0.05)。综上所述，在鱼粉含量为200 g/kg的实用饲料中添加羟脯氨酸和VC，可显著提高凡纳滨对虾在低盐度水中的生长性能和肉质。

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引用次数: 0

Different types of dietary carotenoids improve the color and odor quality of Eriocheir sinensis ovaries 不同种类的类胡萝卜素能改善中华鳖卵巢的颜色和气味质量

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2024.01.001

Long Zhang , Jingjing Wu , Xiaodong Jiang , Xugan Wu , Xichang Wang

Chinese mitten crab, Eriocheir sinensis, is a commercially ecological crab species in China. Ovaries are one of the major edible parts for female E. sinensis, and their color and flavor primarily affect consumer acceptance. This study investigated the effects of different dietary carotenoids on the color and odor quality of adult female E. sinensis ovaries. The crab was fed the experiment diets with supplementation of 100 ×10⁻⁶ of β-carotene, astaxanthin, lutein, canthaxanthin and zeaxanthin, respectively, for 70 days, while the control diet without the supplementation of carotenoids. The results showed that different carotenoids generated an orange-red coloration on crab ovaries, and astaxanthin and canthaxanthin significantly increased the redness (P < 0.05). All carotenoid groups had higher total carotenoids and fatty acid contents than the control group. Moreover, β-carotene significantly promoted ovarian carotenoid deposition, and lutein predominantly affected fatty acid accumulation (P < 0.05). β-carotene and lutein groups accumulated more aroma components (alcohols, ketones, and aldehydes) by regulating the metabolism of carotenoid and fatty acids in ovaries. These results show that canthaxanthin is a potential astaxanthin substitute for promoting coloration, while β-carotene and lutein can improve the odor quality of the ovaries of adult female E. sinensis.

中华绒螯蟹（Eriocheir sinensis）是中国一种商业生态蟹种。子房是雌性中华梭子鱼的主要食用部位之一，其颜色和风味是影响消费者接受度的主要因素。本试验研究了饲料中添加不同的类胡萝卜素对成虫雌性卵巢颜色和气味品质的影响。试验饲粮中分别添加100 ×10−6的β-胡萝卜素、虾青素、叶黄素、角黄素和玉米黄质，饲喂70 d，对照组不添加类胡萝卜素。结果表明，不同的类胡萝卜素使蟹子房呈现橙红色，虾青素和角黄素显著增加了蟹子房的红色(P <；0.05)。所有类胡萝卜素组的总类胡萝卜素和脂肪酸含量均高于对照组。此外，β-胡萝卜素显著促进卵巢类胡萝卜素沉积，叶黄素主要影响脂肪酸积累(P <；0.05)。β-胡萝卜素和叶黄素组通过调节卵巢中类胡萝卜素和脂肪酸的代谢，积累了更多的香气成分（醇、酮和醛）。综上所述，角黄素是一种潜在的虾青素替代品，具有促进显色的作用，而β-胡萝卜素和叶黄素可以改善成虫雌性卵巢的气味质量。

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引用次数: 0

Toxicity and therapeutical impacts of Bee venom (Apis mellifera L.) on Nile tilapia juvenile (Oreochromis niloticus) 蜂毒（Apis mellifera L.）对尼罗罗非鱼幼鱼（Oreochromis niloticus）的毒性和治疗影响

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2024.02.002

Mohammed F. El Basuini , Haitham M. Ramadan , Abdelaziz M. El-Hais , Mohamed A.A. Zaki , Nourhan M. Kamel , Islam I. Teiba , Emad H. El-Bilawy , Mohamed Reda Badr , Mohamed Fathy Abdel-Aziz , Akram Ismael Shehata

Bee venom (BV) has been widely studied for its therapeutic properties in various medical applications. However, its effects on aquatic organisms, particularly Nile tilapia (Oreochromis niloticus), remain poorly understood, particularly concerning toxicity and therapeutic impacts. This study investigated the effects of bee venom (BV) injection on Nile tilapia (O. niloticus) by examining growth, feed efficiency, biometric parameters, whole-body chemical analysis, and the activity of antioxidant enzymes in the liver. Nile tilapia juveniles were injected with BV at doses of 0, 3, 6, 9, and 12 mg/kg of fish weight. Observations were made on the 14th day post-injection (DPI). No significant differences were found in growth, feed utilization, survival rate, biometric indices, and whole-body composition among the BV dosage groups. Similarly, the activity of intestinal enzymes (amylase, protease, and lipase) showed no significant changes. However, the study revealed a time-dependent response of antioxidant enzymes (SOD, CAT, and GPx) in the liver, with activity peaks on the 4th and 6th DPI. On the 14th DPI, fish injected with BV at doses of 3, 6, and 9 mg/kg exhibited higher antioxidant enzyme activities compared to the control and the highest dosage group (12 mg/kg). These results imply the presence of potential adaptive antioxidant activity and a favorable influence on the health and immune responses of Nile tilapia following BV injection. Notably, the effects appear to vary with the dose, influencing both antioxidant enzyme activities and immunity. Further research is warranted to explore the long-term effects of BV exposure and its potential therapeutic applications in aquaculture.

蜂毒（BV）因其治疗特性在各种医学应用中得到了广泛的研究。然而，其对水生生物，特别是尼罗罗非鱼（Oreochromis niloticus）的影响仍然知之甚少，特别是在毒性和治疗影响方面。本研究通过考察尼罗罗非鱼（O. niloticus）的生长、饲料效率、生物特征参数、全身化学分析和肝脏抗氧化酶活性，研究了蜂毒（BV）注射对尼罗罗非鱼（O. niloticus）的影响。将尼罗罗非鱼幼鱼按每公斤鱼重0、3、6、9和12毫克的剂量注射BV。注射后第14天进行观察。BV给药组间生长、饲料利用率、存活率、生物特征指标和全身组成均无显著差异。肠道酶（淀粉酶、蛋白酶和脂肪酶）的活性也无明显变化。然而，该研究揭示了肝脏中抗氧化酶（SOD， CAT和GPx）的时间依赖性反应，其活性在第4和第6 DPI时达到峰值。在第14 DPI时，与对照组和最高剂量组（12 mg/kg）相比，注射3、6和9 mg/kg BV的鱼表现出更高的抗氧化酶活性。这些结果表明BV注射后存在潜在的适应性抗氧化活性，对尼罗罗非鱼的健康和免疫应答有良好的影响。值得注意的是，效果似乎随剂量而变化，影响抗氧化酶活性和免疫力。有必要进一步研究细菌性阴道炎暴露的长期影响及其在水产养殖中的潜在治疗应用。

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引用次数: 0

Transposon insertion in pmel17 rewired skin and muscle transcriptomes in Mozambique tilapia 莫桑比克罗非鱼皮肤和肌肉转录组中pmel17转座子的插入

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2023.12.002

Fei Sun , Le Wang , Gen Hua Yue

The pmel17 gene plays a crucial role in melanin pigmentation. Our previous studies showed that in Mozambique tilapia, a transposon inserted into the 3′ untranslated region (3′ UTR) of pmel17 resulted in the silencing of pmel17 and led to the loss of melanin pigments (golden mutant phenotype). Additionally, the transposon insertion caused reduced growth performance and increased locomotion. In this study, to investigate the mechanisms underlying these phenotypic changes, we sequenced transcriptomes of the skin and muscle samples collected from wildtype and mutant tilapias. A total of 51 and 141 differentially expressed genes (DEGs) were identified in the skin and muscle transcriptomes, respectively. DEGs in the skin were primarily down-regulated in golden genotypes and associated with neural crest development and melanin pigmentation pathways. Besides these DEGs involved in the classic melanin pigmentation pathway of vertebrates, 14 DEGs were also observed to be related to melanogenesis. In muscle transcriptomes, there was an enrichment of GO terms associated with growth factors and cellular lipid catabolic processes. Specifically, DEGs related to growth factor binding exhibited a down-regulation, while those related to lipid metabolism showed an up-regulation in mutant genotypes. These findings agree with observed phenotypic changes. Furthermore, several DEGs associated with muscle function and mobility were up-regulated. Our study sheds light on how a single mutation in a gene can modulate multiple phenotypes by rewiring gene regulation networks. The research also provides valuable insights into the complex genetic mechanisms underlying the regulation of diverse phenotypic traits by a single gene.

pmel17基因在黑色素沉着中起着关键作用。我们之前的研究表明，在莫桑比克罗非鱼中，一个转座子插入pmel17的3 ‘非翻译区（3 ’ UTR），导致pmel17沉默，导致黑色素色素的损失（黄金突变表型）。此外，转座子插入导致生长性能下降和运动增加。在这项研究中，为了研究这些表型变化的机制，我们对野生型和突变型罗非鱼的皮肤和肌肉样本的转录组进行了测序。在皮肤和肌肉转录组中分别鉴定出51个和141个差异表达基因（deg）。在金色基因型中，皮肤中的DEGs主要下调，并与神经嵴发育和黑色素色素沉着途径相关。除了这些基因参与经典的脊椎动物黑色素沉着途径外，还观察到14个基因与黑色素形成有关。在肌肉转录组中，与生长因子和细胞脂质分解代谢过程相关的氧化石墨烯术语富集。具体来说，在突变基因型中，与生长因子结合相关的deg表现为下调，而与脂质代谢相关的deg表现为上调。这些发现与观察到的表型变化一致。此外，一些与肌肉功能和活动相关的deg被上调。我们的研究揭示了基因中的单个突变如何通过重新连接基因调控网络来调节多种表型。该研究还为单个基因调控多种表型性状的复杂遗传机制提供了有价值的见解。

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引用次数: 0

Domestication of manggabai fish (Glossogobius giuris) through adaptation to different types of feed and aquaculture containers 通过适应不同类型的饲料和水产养殖容器驯化芒加贝鱼（Glossogobius giuris）。

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries

Pub Date : 2025-05-01 DOI: 10.1016/j.aaf.2023.12.005

Yuniarti Koniyo, Juliana

This study aims to determine the growth and survival of Manggabai fish (Glossogobius giuris) cultivated in different types of feed and maintenance containers. The method used in the study was an experimental method using a 2 × 3 Factorial Complete Randomized Design (CRD), namely two levels of rearing container factors and three levels of feed type factors. The container consists of two types of containers, namely aquarium and concrete tub. The feed given consists of three types, namely Daphnia sp., Tubifex sp. And pellets. The research variables consisted of growth and survival of test animals. The test animals used were Manggabai fish with a total length of (3 ± 0.05) cm and body weight of 4 ± 0.05 g. Data analysis in this study used a two-factor Completely Randomized Design with a probability level of P < 0.05 and used descriptive analysis of length, weight, and survival growth data during the maintenance of test animals. The results showed that the use of different feed and maintenance containers had a significant effect on the growth and survival of manggabai fish. Total absolute weight growth of manggabai fish reared in concrete containers and fed with Daphnia sp. 22.19 g, pellets 22.99 g, and those fed with Tubifex sp. Feed amounted to 39.63 g. The highest growth and survival were obtained in the treatment using a concrete tank and Tubifex sp. Feed. The highest growth and survival were obtained in the treatment using a concrete tank and Tubifex sp. Feed. The use of aquarium maintenance containers and the provision of pellets is not recommended for domestication of Manggabai fish, because it provides the lowest growth and survival.

本研究旨在确定在不同类型的饲料和维持容器中养殖的曼加白鱼（Glossogobius giuris）的生长和存活情况。本研究采用2 × 3全因子完全随机设计（CRD）的试验方法，即2个水平的饲养容器因素和3个水平的饲料类型因素。该容器由两种容器组成，即水族箱和混凝土桶。所给的饲料有三种，即水蚤、管蚤和颗粒。研究变量包括试验动物的生长和存活。试验动物为长（3±0.05）cm、体重4±0.05 g的芒嘎白鱼。本研究的数据分析采用双因素完全随机设计，概率水平为P <；0.05，并对试验动物维持期间的长度、体重和存活生长数据进行描述性分析。结果表明，使用不同的饲料和维持容器对芒加白鱼的生长和存活有显著影响。在混凝土容器中饲养，水蚤饲料22.19 g，颗粒饲料22.99 g， Tubifex饲料39.63 g。用混凝土池和Tubifex sp.饲料处理的生长和成活率最高。用混凝土池和Tubifex sp.饲料处理的生长和成活率最高。不建议使用水族馆养护容器和提供颗粒来驯化mangabbai鱼，因为它提供的生长和存活率最低。

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引用次数: 0