Pub Date : 2022-08-08DOI: 10.1109/3M-NANO56083.2022.9941512
Jiaxiang Zhu, Guangbo Hao, Hui Tang
This article presents two new compliant mechanisms that are able to achieve bi-stability and static balancing where the improved motion characteristics and reduced footprint can be obtained. One design is a single-layer symmetrical design, and the other design is a two-layer compact design. Static balancing is accomplished by adjusting the initial slope of the beam embodied in the bistable mechanism. The free-body diagram (FBD) combined with the beam constraint model (BCM) method is employed to capture the nonlinearities in the force-displacement characteristics of the proposed compliant bistable mechanism. Nonlinear Finite element analysis (FEA) is carried out to verify the modelling results.
{"title":"Design and Modelling of a Generic Compliant Mechanism with Bi-stability and Static Balancing","authors":"Jiaxiang Zhu, Guangbo Hao, Hui Tang","doi":"10.1109/3M-NANO56083.2022.9941512","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941512","url":null,"abstract":"This article presents two new compliant mechanisms that are able to achieve bi-stability and static balancing where the improved motion characteristics and reduced footprint can be obtained. One design is a single-layer symmetrical design, and the other design is a two-layer compact design. Static balancing is accomplished by adjusting the initial slope of the beam embodied in the bistable mechanism. The free-body diagram (FBD) combined with the beam constraint model (BCM) method is employed to capture the nonlinearities in the force-displacement characteristics of the proposed compliant bistable mechanism. Nonlinear Finite element analysis (FEA) is carried out to verify the modelling results.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125827603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-08DOI: 10.1109/3M-NANO56083.2022.9941701
Y. Nie, Xinkai Zhao, Hong Bin
Optical free-form surfaces are widely used in various fields. Ultra-precision fast tool servo technology is commonly adopted to process free-form optics. However, the surface decomposition algorithm and smoothing algorithm are immature. In this paper, the cutting points of tool path are endowed with timing characteristic based on machining parameters. Thus, the machining path can be put in a quasi four dimensional coordinate system. Then a tool path generation method based on the signal filtering technology is proposed to realize the surface decomposition and path smoothing, which can make better use of the characteristics of each machining axis of the machine tool and achieve better machining accuracy at the same time.
{"title":"Tool Path Generation of FTS Machined Freeform Optics Based on Filtering in Quasi-4D Coordinate Systems","authors":"Y. Nie, Xinkai Zhao, Hong Bin","doi":"10.1109/3M-NANO56083.2022.9941701","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941701","url":null,"abstract":"Optical free-form surfaces are widely used in various fields. Ultra-precision fast tool servo technology is commonly adopted to process free-form optics. However, the surface decomposition algorithm and smoothing algorithm are immature. In this paper, the cutting points of tool path are endowed with timing characteristic based on machining parameters. Thus, the machining path can be put in a quasi four dimensional coordinate system. Then a tool path generation method based on the signal filtering technology is proposed to realize the surface decomposition and path smoothing, which can make better use of the characteristics of each machining axis of the machine tool and achieve better machining accuracy at the same time.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125488397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-08DOI: 10.1109/3M-NANO56083.2022.9941587
Yang Yang, Bosen Chai, Miao Miao
With the deepening of educational reform, the mechanism is explained from the molecular level, and the knowledge of mechanical science at the forefront of scientific research is integrated into classroom teaching. In this paper, Molecular Dynamic simulations was used to study the process of polymer brushes formed by the attached of polymer chains on polymer surface. The surface adsorption densities were distinguished according to different chain structures and counter ions. Two different modes are considered: (a) various the A-block lengths at fixed the chain length; (b) various the chain lengths at fixed the A-block length. The surface adsorption potential energy plays an important role in the shape of the diblock copolymer chain and the formation of the brush. The adsorption density studied in this paper can provide a basis for experimenters to design polymer brushes with required properties.
{"title":"Research on Educational Reform of Versatile Diblock Copolymers Confined on the Face Centered Cubic Crystal","authors":"Yang Yang, Bosen Chai, Miao Miao","doi":"10.1109/3M-NANO56083.2022.9941587","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941587","url":null,"abstract":"With the deepening of educational reform, the mechanism is explained from the molecular level, and the knowledge of mechanical science at the forefront of scientific research is integrated into classroom teaching. In this paper, Molecular Dynamic simulations was used to study the process of polymer brushes formed by the attached of polymer chains on polymer surface. The surface adsorption densities were distinguished according to different chain structures and counter ions. Two different modes are considered: (a) various the A-block lengths at fixed the chain length; (b) various the chain lengths at fixed the A-block length. The surface adsorption potential energy plays an important role in the shape of the diblock copolymer chain and the formation of the brush. The adsorption density studied in this paper can provide a basis for experimenters to design polymer brushes with required properties.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126359807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During a three-sided pyramidal tip based nanoscratching process, the cutting force on the tip varies with the machining direction. When the lateral force on the tip is large, it can lead to lateral deformation of the grooves and limits further fabrication of 2D and 3D microstructures. In order to obtain the machining depth of the grooves when it is not deformed, we analyzed the relationship between the normal load applied to the tip and the scratching depth. The results indicated that the experimental scratching depth matches well with the theoretical when 0° ≤ θ ≤ 20° and 35° ≤ θ ≤ 60°.≤ is the angle between the horizontal projection of the three-sided pyramid edge AD and the direction of scratching. When θ = 0°, the edge AD faces forward during the scratching process Furthermore, the force on the tip was also studied when machining in different directions. According to theoretical analysis and experimental testing, grooves would deform when the force vertical to scratching direction on the tip was greater than 30µN. Finally, we obtained the relationship between the maximum depth and machining direction during nanoscratching process.
{"title":"Study of the Cutting Depth and Forces of the Three-sided Pyramid Tip for Nanoscratching Process","authors":"Xiliang Tang, Yanling Tian, Weijie Wang, Guanghui Zhao, Dawei Zhang","doi":"10.1109/3M-NANO56083.2022.9941365","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941365","url":null,"abstract":"During a three-sided pyramidal tip based nanoscratching process, the cutting force on the tip varies with the machining direction. When the lateral force on the tip is large, it can lead to lateral deformation of the grooves and limits further fabrication of 2D and 3D microstructures. In order to obtain the machining depth of the grooves when it is not deformed, we analyzed the relationship between the normal load applied to the tip and the scratching depth. The results indicated that the experimental scratching depth matches well with the theoretical when 0° ≤ θ ≤ 20° and 35° ≤ θ ≤ 60°.≤ is the angle between the horizontal projection of the three-sided pyramid edge AD and the direction of scratching. When θ = 0°, the edge AD faces forward during the scratching process Furthermore, the force on the tip was also studied when machining in different directions. According to theoretical analysis and experimental testing, grooves would deform when the force vertical to scratching direction on the tip was greater than 30µN. Finally, we obtained the relationship between the maximum depth and machining direction during nanoscratching process.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128377515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-08DOI: 10.1109/3M-NANO56083.2022.9941548
Zhendong Zhu, Wei Si
As an important way to manipulate microfluidics, electroosmosis has attracted extensive attention of researchers at home and abroad. In this paper, the electroosmotic drive of double nanopore structure is studied. The two nanopores are set with opposite charges and the applied electric field is used. With the software VMD and NAMD2, MD simulation of the double nanopore structure gets realized in the work. The ion concentration distribution, electric field streamline change and potential distribution of the system have been studied in this research to explain the mechanism of electroosmotic flow. This structure could be applied in many micro nano situations in the future.
{"title":"Analysis of Electroosmotic Drive of Double Nanopore Structure","authors":"Zhendong Zhu, Wei Si","doi":"10.1109/3M-NANO56083.2022.9941548","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941548","url":null,"abstract":"As an important way to manipulate microfluidics, electroosmosis has attracted extensive attention of researchers at home and abroad. In this paper, the electroosmotic drive of double nanopore structure is studied. The two nanopores are set with opposite charges and the applied electric field is used. With the software VMD and NAMD2, MD simulation of the double nanopore structure gets realized in the work. The ion concentration distribution, electric field streamline change and potential distribution of the system have been studied in this research to explain the mechanism of electroosmotic flow. This structure could be applied in many micro nano situations in the future.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132239605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-08DOI: 10.1109/3M-NANO56083.2022.9941596
Shen Wang, Jinkai Xu, G. Chen
SiCp/Al composites have light weight and high hardness and are widely applied in aerospace, automobile, motor engineer and other fields. In this paper, the finite element model of a single particle is set up by finite element software. Comparing the influence of ultrasonic amplitude, it can be concluded that the application of ultrasonic vibration can change the moving trajectory of the tool and change the material removal principle, thereby reducing the defects on the machined surface. Through a series of experiment and surface measurement, the machined quality is improved a lot when the ultrasonic vibration is applied. When the ultrasonic amplitude is $0 mumathrm{m}, 3 mumathrm{m}, 6mumathrm{m}$, the surface roughness is $0.183 mumathrm{m}, 0.065 mumathrm{m},0.389 mumathrm{m}$. The surface defects are reduced greatly.
SiCp/Al复合材料具有重量轻、硬度高的特点，广泛应用于航空航天、汽车、电机工程等领域。本文利用有限元软件建立了单粒子的有限元模型。对比超声波振幅的影响，可以得出超声波振动的应用可以改变刀具的运动轨迹，改变材料的去除原理，从而减少加工表面的缺陷。通过一系列的实验和表面测量，超声波振动对工件的加工质量有很大的改善。当超声振幅为$0 mu mathm {m}、 3 mu mathm {m}、 6mu mathm {m}$时，表面粗糙度分别为$0.183 mu mathm {m}、 0.065 mu mathm {m}、0.389 mu mathm {m}$。表面缺陷大大减少。
{"title":"Study on the Simulation of Ultrasonic Vibration-assisted Milling on SiCp/Al","authors":"Shen Wang, Jinkai Xu, G. Chen","doi":"10.1109/3M-NANO56083.2022.9941596","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941596","url":null,"abstract":"SiCp/Al composites have light weight and high hardness and are widely applied in aerospace, automobile, motor engineer and other fields. In this paper, the finite element model of a single particle is set up by finite element software. Comparing the influence of ultrasonic amplitude, it can be concluded that the application of ultrasonic vibration can change the moving trajectory of the tool and change the material removal principle, thereby reducing the defects on the machined surface. Through a series of experiment and surface measurement, the machined quality is improved a lot when the ultrasonic vibration is applied. When the ultrasonic amplitude is $0 mumathrm{m}, 3 mumathrm{m}, 6mumathrm{m}$, the surface roughness is $0.183 mumathrm{m}, 0.065 mumathrm{m},0.389 mumathrm{m}$. The surface defects are reduced greatly.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122846563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The heat generation of the precision ball screw feed system affects the preload and axial stiffness, which in turn changes the dynamic characteristics of the feed system. Therefore, a thermodynamic model is needed to study the change of dynamic performance with temperature rise. In this paper, the axial stiffness model of the feed system is established. Based on the heat transfer theory, considering the influence of thermal deformation on the preload and axial stiffness of key components, a thermodynamic coupling model of the feed system is established, and numerical simulation analysis is carried out. It provides a theoretical basis for real-time monitoring and control of the dynamic characteristics of the feed system.
{"title":"Thermal-dynamic Coupling Modeling and Simulation Analysis of Precision Feed System","authors":"Zhikai Fu, Weiguo Gao, Faze Chen, Haoxiong Sun, Xiaohui Li, Dawei Zhang, Yuandong Tian","doi":"10.1109/3M-NANO56083.2022.9941566","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941566","url":null,"abstract":"The heat generation of the precision ball screw feed system affects the preload and axial stiffness, which in turn changes the dynamic characteristics of the feed system. Therefore, a thermodynamic model is needed to study the change of dynamic performance with temperature rise. In this paper, the axial stiffness model of the feed system is established. Based on the heat transfer theory, considering the influence of thermal deformation on the preload and axial stiffness of key components, a thermodynamic coupling model of the feed system is established, and numerical simulation analysis is carried out. It provides a theoretical basis for real-time monitoring and control of the dynamic characteristics of the feed system.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127949279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carbon fiber reinforced polymer (CFRP) has been widely used in recent years due to their excellent mechanical properties, especially in aerospace and automotive fields. However, the low bonding strength of the CFRP due to its low surface energy restrains its industrial applications. Although methods including mechanical treatment, laser treatment, flame treatment and chemical treatment have been proposed to improve the bonding strength, the methods tend to involve dangerous chemical reagents, or may induce serious structural damages. In this study, we propose to use atmospheric pressure plasma jet (APPJ) to improve the surface wettability and bonding strength of the CFRP. Contact angles of water and epoxy resin, surface chemical composition, and microstructures before and after the APPJ treatments are characterized. Results indicate that the APPJ treatment can effectively improve surface wettability and bonding strength of the CFRP. The water contact angle decreases from ∼86° to ∼15.2°, while the shear strength increases from ∼5.5 MPa to ∼26.9 MPa, indicating that the APPJ may be a promising candidate for treatment of CFRP surfaces.
{"title":"Improving the Bonding Strength of CFRP by Atmospheric Pressure Plasma Jet","authors":"Xin Liu, Jingcan Yan, Faze Chen, Bingqi Wang, Yuheng Li, Jiyu Liu","doi":"10.1109/3M-NANO56083.2022.9941616","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941616","url":null,"abstract":"Carbon fiber reinforced polymer (CFRP) has been widely used in recent years due to their excellent mechanical properties, especially in aerospace and automotive fields. However, the low bonding strength of the CFRP due to its low surface energy restrains its industrial applications. Although methods including mechanical treatment, laser treatment, flame treatment and chemical treatment have been proposed to improve the bonding strength, the methods tend to involve dangerous chemical reagents, or may induce serious structural damages. In this study, we propose to use atmospheric pressure plasma jet (APPJ) to improve the surface wettability and bonding strength of the CFRP. Contact angles of water and epoxy resin, surface chemical composition, and microstructures before and after the APPJ treatments are characterized. Results indicate that the APPJ treatment can effectively improve surface wettability and bonding strength of the CFRP. The water contact angle decreases from ∼86° to ∼15.2°, while the shear strength increases from ∼5.5 MPa to ∼26.9 MPa, indicating that the APPJ may be a promising candidate for treatment of CFRP surfaces.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116817970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-08DOI: 10.1109/3M-NANO56083.2022.9941721
Sadaf Saeed, Ri Liu, M. Gao, Dongdong Liu, Zuobin Wang, Ali Zia
In this work, we represent an elegant way for the fabrication of hybrid Si nanowires and nanoholes (NWs-NHS) arrays. In this article, we characterized the array structures and obtained their periods, diameters and etching depths by collaborating the laser interference lithography (LIL) and metal-assisted chemical etching (MACE). First of all, silver (Ag) films were placed on Silicon (Si) substrates, and a 1064 nm high power laser source was used. Afterward, MACE was done for the fabrication of hybrid Si (NWs-Hs) arrays. The images with the surface characteristics were obtained by scanning electron microscope (SEM). This work offers a simple, less time-consuming, and cost-effective process of micro-nano pattern fabrication.
{"title":"Hierarchical and Gradient Si Nano Wires-holes Arrays by LIL and MACE","authors":"Sadaf Saeed, Ri Liu, M. Gao, Dongdong Liu, Zuobin Wang, Ali Zia","doi":"10.1109/3M-NANO56083.2022.9941721","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941721","url":null,"abstract":"In this work, we represent an elegant way for the fabrication of hybrid Si nanowires and nanoholes (NWs-NHS) arrays. In this article, we characterized the array structures and obtained their periods, diameters and etching depths by collaborating the laser interference lithography (LIL) and metal-assisted chemical etching (MACE). First of all, silver (Ag) films were placed on Silicon (Si) substrates, and a 1064 nm high power laser source was used. Afterward, MACE was done for the fabrication of hybrid Si (NWs-Hs) arrays. The images with the surface characteristics were obtained by scanning electron microscope (SEM). This work offers a simple, less time-consuming, and cost-effective process of micro-nano pattern fabrication.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117001317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mast cells play an important role in the development of allergic disease, and RBL-2H3 cells, a subsystem of rat basophilic leukocytes (RBL), can express many of the properties and functions of mast cells. The use of atomic force microscope (AFM) in biology has gained increasing attention due to its high resolution and the fact that it can be manipulated and observed under near-physiological conditions. However, the physical changes of cell degranulation based on AFM still need to be studied. In this paper, the changes of cell physical parameters and cell membranes of RBL-2H3 cells during cell degranulation induced by anti-DNP IgE were recorded, and the recorded data were analysed. The results showed that the effect of anti-DNP IgE on RBL-2H3 cells varied with time, peaking at 8–12 hours.
肥大细胞在过敏性疾病的发生发展中起着重要作用，RBL- 2h3细胞是大鼠嗜碱性白细胞(RBL)的一个子系统，可以表达肥大细胞的许多特性和功能。原子力显微镜(atomic force microscope, AFM)因其高分辨率和可在接近生理条件下操作和观察而在生物学中的应用越来越受到关注。然而，基于AFM的细胞脱颗粒的物理变化仍有待研究。本文记录抗dnp IgE诱导RBL-2H3细胞脱颗粒过程中细胞物理参数和细胞膜的变化，并对记录数据进行分析。结果表明，抗dnp IgE对RBL-2H3细胞的作用随时间的变化而变化，在8-12 h达到峰值。
{"title":"Imaging of RBL-2H3 Cell Degranulation by Atomic Force Microscopy","authors":"Jiani Li, Bowei Wang, Jianjun Dong, Ying Wang, Kaige Qu, Yujuan Chen, Zuobin Wang","doi":"10.1109/3M-NANO56083.2022.9941640","DOIUrl":"https://doi.org/10.1109/3M-NANO56083.2022.9941640","url":null,"abstract":"Mast cells play an important role in the development of allergic disease, and RBL-2H3 cells, a subsystem of rat basophilic leukocytes (RBL), can express many of the properties and functions of mast cells. The use of atomic force microscope (AFM) in biology has gained increasing attention due to its high resolution and the fact that it can be manipulated and observed under near-physiological conditions. However, the physical changes of cell degranulation based on AFM still need to be studied. In this paper, the changes of cell physical parameters and cell membranes of RBL-2H3 cells during cell degranulation induced by anti-DNP IgE were recorded, and the recorded data were analysed. The results showed that the effect of anti-DNP IgE on RBL-2H3 cells varied with time, peaking at 8–12 hours.","PeriodicalId":370631,"journal":{"name":"2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126605277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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