Bioprinting最新文献_第5页

4D bioprinting: Materials, mechanisms, and mathematical modeling for next-generation tissue engineering 4D生物打印：下一代组织工程的材料、机制和数学建模

Q1 Computer Science

Bioprinting

Pub Date : 2025-07-17 DOI: 10.1016/j.bprint.2025.e00428

Faezeh Raei , Azadeh Abdi , Shohreh Mashayekhan

Advances in 3D bioprinting have enabled the fabrication of synthetic tissues with complex architectures that closely mimic natural ones. However, 3D bioprinting faces challenges in generating fully functional bioconstructs using biocompatible materials and cells. To overcome this limitation, the emerging technology of 4D bioprinting offers a novel solution. Unlike its 3D counterpart, 4D bioprinting enables structures to change shape in response to both intrinsic and external stimuli. This dynamic capability of 4D bioprinting has the potential to surpass the limitations of 3D bioprinting while more accurately replicating the adaptive nature of living tissues. By leveraging 4D bioprinting, it becomes feasible to produce highly intricate and dynamic structures with exceptional resolution, which would be challenging to achieve using conventional biofabrication methods such as 3D printing or bioprinting. This review highlights the applications of stimuli-responsive materials in 4D bioprinting. It delves into the chemistry and mechanism of action of advanced 4D materials. Additionally, this review discusses the diverse applications of 4D bioprinted tissues and organs, emphasizing their impact on regenerative medicine. The integration of mathematical modeling as a predictive tool for the printing process and final structural outcomes is also examined. Furthermore, the article addresses essential testing protocols for evaluating the functionality and safety of bioprinted tissues. Finally, it discusses current challenges and future directions in this rapidly evolving field, particularly its implications and potential breakthroughs in tissue engineering.

生物3D打印技术的进步使制造具有复杂结构的合成组织成为可能，这些组织与自然组织非常相似。然而，3D生物打印在使用生物相容性材料和细胞生成功能齐全的生物结构方面面临挑战。为了克服这一限制，新兴的4D生物打印技术提供了一种新的解决方案。与3D打印不同，4D生物打印使结构能够根据内部和外部刺激改变形状。4D生物打印的这种动态能力有可能超越3D生物打印的局限性，同时更准确地复制活组织的适应性。通过利用4D生物打印，可以以特殊的分辨率生产高度复杂和动态的结构，这将是使用传统的生物制造方法（如3D打印或生物打印）难以实现的。本文综述了刺激响应材料在4D生物打印中的应用。深入研究了先进4D材料的化学性质和作用机理。此外，本文还讨论了4D生物打印组织和器官的各种应用，强调了它们在再生医学中的影响。将数学建模作为印刷过程和最终结构结果的预测工具的集成也进行了检查。此外，本文还讨论了评估生物打印组织的功能和安全性的基本测试方案。最后，它讨论了当前的挑战和未来的方向，在这个快速发展的领域，特别是其影响和潜在的突破组织工程。

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引用次数: 0

Enhancing the printability of low-concentration GelMA through viscosity modulation and integration of hydroxyapatite for bone tissue engineering bioinks 通过粘度调节和羟基磷灰石的整合来提高骨组织工程生物墨水的低浓度GelMA的可打印性

Q1 Computer Science

Bioprinting

Pub Date : 2025-07-17 DOI: 10.1016/j.bprint.2025.e00426

Soumitra Das , Anne Bernhardt , Michael Gelinsky , Bikramjit Basu

In recent years, there has been a significant focus on developing hydrogel-based scaffolds for reconstructing and repairing damaged tissues. Despite these efforts, the selection of appropriate hydrogel formulation tailored to specific clinical applications remains a primary challenge. Gelatin methacryloyl (GelMA) has been widely investigated as a baseline biomaterial in the realm of tissue engineering. Through comprehensive experimentation and quantitative analysis, we explore the intricate interplay among various biophysical properties (uniaxial compression behavior, scaffold microstructure, swelling properties, and enzymatic degradation kinetics), viscoelastic properties, printability, and cellular responses of a range of GelMA compositions. The experimental data were comprehensively analyzed to establish an empirical relationship between biophysical properties and molar crosslinking density. In particular, the viscoelastic properties were tailored for low-concentration GelMA, containing biomineralized bone-specific biomaterial ink by tailoring the addition of methacrylated carboxymethyl cellulose (mCMC), and nanocrystalline hydroxyapatite (nHAp). The resulting hybrid hydrogel demonstrates significantly higher stiffness (∼7-fold), improved yield stress (∼17-fold), reduced swelling (∼1.3-fold), and diminished degradation (∼4-fold) properties compared to pristine GelMA. To assess the bone mimetic tissue matrix development, we conducted 2D cultures of human patient-derived primary bone marrow mesenchymal stem cells (hBMSCs) and human osteoblasts (hOBs) on hydrogel scaffolds in standard growth media and differentiation media. Our results qualitatively and quantitatively indicate robust proliferation of both cell types on all biomaterial scaffolds over 21 days in culture. Furthermore, an analysis of alkaline phosphatase (ALP) activity reveals a ∼3.1-fold and ∼5.8-fold increase in ALP expression for hBMSCs-seeded nHAp-loaded hydrogels, cultured in non-differentiation media and differentiation media, respectively. Taken together, our findings suggest that the nHAp-incorporated GelMA/mCMC matrix holds promise as a potential biomaterial ink for bone tissue regeneration applications.

近年来，开发基于水凝胶的支架来重建和修复受损组织已成为人们关注的焦点。尽管做出了这些努力，但根据具体临床应用选择合适的水凝胶配方仍然是一个主要挑战。明胶甲基丙烯酰（GelMA）作为一种基础生物材料在组织工程领域得到了广泛的研究。通过全面的实验和定量分析，我们探索了各种生物物理特性（单轴压缩行为、支架微观结构、膨胀特性和酶降解动力学）、粘弹性特性、可打印性和一系列GelMA成分的细胞反应之间复杂的相互作用。对实验数据进行综合分析，建立生物物理性质与摩尔交联密度之间的经验关系。特别是，通过添加甲基丙烯酸羧甲基纤维素（mCMC）和纳米羟基磷灰石（nHAp），为低浓度GelMA量身定制了粘弹性性能。GelMA含有生物矿化骨特异性生物材料墨水。与原始GelMA相比，所得到的杂交水凝胶具有显着更高的刚度（~ 7倍），改善的屈服应力（~ 17倍），减少的肿胀（~ 1.3倍）和减少的降解（~ 4倍）性能。为了评估骨模拟组织基质的发育，我们在标准生长培养基和分化培养基的水凝胶支架上对人患者来源的原代骨髓间充质干细胞（hBMSCs）和人成骨细胞（hOBs）进行了二维培养。我们的结果定性和定量地表明，在21天的培养中，两种细胞类型在所有生物材料支架上都有强劲的增殖。此外，碱性磷酸酶（ALP）活性分析显示，在非分化培养基和分化培养基中培养的hbmscs - nhap负载水凝胶中，ALP表达分别增加了~ 3.1倍和~ 5.8倍。综上所述，我们的研究结果表明，nhap结合的GelMA/mCMC基质有望成为骨组织再生应用的潜在生物材料墨水。

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引用次数: 0

LusoBioMaker: A low-cost 3D bioprinter with multi-extrusion and contour printing capabilities for thermo- and photocurable hydrogels towards complex tissue fabrication LusoBioMaker：一种低成本的生物3D打印机，具有多挤出和轮廓打印能力，可用于热固化和光固化水凝胶，用于复杂的组织制造

Q1 Computer Science

Bioprinting

Pub Date : 2025-07-05 DOI: 10.1016/j.bprint.2025.e00425

Afonso Gusmão , Diana M.C. Marques , Duarte Almeida , Kristin Schüler , Frederico Castelo Ferreira , Paola Sanjuan-Alberte , Marco Leite

3D bioprinting is an expanding field that allows for the design of intricate structures using multiple materials and living cells. This has enormous potential for applications in drug testing, regenerative medicine, and, more recently, cell-based food products, with the surge of the cellular agriculture field. However, the high cost of equipment is frequently a significant limitation for implementing these approaches. Here, we present LusoBioMaker, an open-source bioprinter that delivers commercial-grade performance for under $900 of materi. Built on a modified Ender 3-V2 platform it integrates dual screw-driven extrusion, independent active temperature control (2–50 °C) and in-situ 365 nm photocuring, generating up to 320 N force through open-access firmware. Using κ-carrageenan, Pluronic F-127 and gelatin methacrylate/poly(ethyleneglycol) diacrylate (GelMA/PEGDA) inks we printed complex lattices with a printability factor of 0.995 and sub-millimetre dimensional errors while maintaining 97 % L929 cell viability after fourteen days. Comprehensive calibration and acceptance tests performed in accordance with the ISO 230-1/2 standards confirmed <50 μm positional error and <0.005° angular deviation across both extrusion nozzles. A systematic review of 17 reported low-cost bioprinters revealed that none combine dual screw extrusion, active thermal regulation and on-head UV curing in a single chassis, highlighting LusoBioMaker's unique features set. As a proof-of-concept, we bioprinted a hollow nipple–areola complex by co-extruding a thermosensitive κ-carrageenan core and a photocurable GelMA/PEGDA shell, exploiting all three hardware capabilities in one uninterrupted run. This demonstration underscores LusoBioMaker's capacity to manufacture anatomically intricate, gradient tissues on demand and to democratise advanced biofabrication workflows.

3D生物打印是一个不断发展的领域，它允许使用多种材料和活细胞设计复杂的结构。随着细胞农业领域的蓬勃发展，这在药物测试、再生医学以及最近以细胞为基础的食品方面具有巨大的应用潜力。然而，设备的高成本往往是实施这些方法的一个重大限制。在这里，我们介绍LusoBioMaker，一个开源的生物打印机，提供商业级的性能低于900美元的材料。它建立在改进的Ender 3-V2平台上，集成了双螺杆驱动挤出，独立主动温度控制（2-50°C）和365 nm原位光固化，通过开放获取固件产生高达320 N的力。我们使用κ-卡拉胶、Pluronic F-127和甲基丙烯酸明胶/聚乙二醇二丙烯酸酯（GelMA/PEGDA）油墨打印复杂晶格，打印因子为0.995，尺寸误差为亚毫米，14天后L929细胞存活率保持在97%。根据ISO 230-1/2标准进行的全面校准和验收测试确认，两个挤出喷嘴的位置误差为50 μm，角偏差为0.005°。对17种低成本生物打印机的系统回顾显示，没有一种将双螺杆挤出、主动热调节和头部UV固化结合在一个机箱中，这突出了LusoBioMaker的独特功能。作为概念验证，我们通过共挤出热敏的β -卡拉胶核和光固化的GelMA/PEGDA壳，在一次不间断的运行中利用了所有三种硬件功能，打印出了空心乳头-乳晕复合物。这次演示强调了LusoBioMaker的能力，以制造解剖复杂，梯度组织的需求和民主化先进的生物制造工作流程。

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引用次数: 0

3D bioprinted neural progenitor cell constructs for enhancing ex vivo brain integration and neuron-astrocyte differentiation 3D生物打印神经祖细胞构建增强离体脑整合和神经元-星形胶质细胞分化

Q1 Computer Science

Bioprinting

Pub Date : 2025-07-02 DOI: 10.1016/j.bprint.2025.e00424

Hui Ling Ma, Raiane de Oliveira Ferreira, Danyllo Felipe de Oliveira, Alice Kei Endo, Oswaldo Keith Okamoto, Mayana Zatz

Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (hiPSCs) hold great promise for neural tissue engineering, disease modeling, and regenerative therapies due to their self-renewal and differentiation potential. In this study, we utilized 3D extrusion bioprinting to encapsulate hiPSC-NPCs within a composite bioink composed of Gelatin methacryloyl (GelMA) and Pluronic F127 (P-127). This composite was engineered to enhance matrix remodeling, mechanical tunability, and cell-specific differentiation. Incorporating P-127 improved gelation, printability, swelling behavior, degradation kinetics, and microstructural features, collectively supporting enhanced NPC proliferation. Mechanical characterization revealed adjustable stiffness (Young's modulus: 1–8 kPa), with GelMA/P-127 blends exhibiting greater strength than GelMA alone. Immunostaining showed elevated GFAP and reduced Neurofilament M (NeuF-M) expression, indicating a shift toward astrocytic differentiation influenced by matrix mechanics. Calcium imaging and transient signal analysis confirmed the functional activity of the differentiated neurons. Gene expression profiling supported these findings, showing upregulation of GFAP, TUBB3, and MAP2 and downregulation of SOX2, marking the transition from progenitor to mature neural phenotypes. Furthermore, bioprinted constructs integrated with ex vivo brain slices and expressed TUBB3 and NeuF-M, confirming neuronal differentiation capacity. These results underscore the potential of GelMA/P-127 composite bioinks as biomimetic, tunable platforms for engineering 3D neural tissue constructs, offering a versatile tool for studying neurodevelopment and advancing translational regenerative strategies.

来源于人诱导多能干细胞（hiPSCs）的神经祖细胞（npc）由于其自我更新和分化的潜力，在神经组织工程、疾病建模和再生治疗方面具有很大的前景。在这项研究中，我们利用3D挤出生物打印技术将hipsc - npc封装在由明胶甲基丙烯酰（GelMA）和Pluronic F127 （P-127）组成的复合生物墨水中。这种复合材料被设计成增强基质重塑、机械可调性和细胞特异性分化。结合P-127改善凝胶性、可打印性、肿胀行为、降解动力学和微观结构特征，共同支持增强鼻咽癌增殖。力学特性显示可调节的刚度（杨氏模量：1-8 kPa）， GelMA/P-127共混物比单独GelMA表现出更高的强度。免疫染色显示GFAP升高，神经丝M （NeuF-M）表达降低，表明在基质力学的影响下向星形细胞分化转变。钙成像和瞬态信号分析证实了分化神经元的功能活性。基因表达谱支持这些发现，显示GFAP、TUBB3和MAP2上调，SOX2下调，标志着从祖神经表型向成熟神经表型的转变。此外，生物打印构建体与离体脑切片结合，表达TUBB3和NeuF-M，证实了神经元分化能力。这些结果强调了GelMA/P-127复合生物墨水作为工程3D神经组织构建的仿生、可调平台的潜力，为研究神经发育和推进转化再生策略提供了一种多功能工具。

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引用次数: 0

3D bioprinting of bioproduction cell lines 生物生产细胞系的3D生物打印

Q1 Computer Science

Bioprinting

Pub Date : 2025-06-27 DOI: 10.1016/j.bprint.2025.e00423

Laura Chastagnier , Lucie Essayan , Celine Thomann , Julia Niemann , Elisabeth Errazuriz-Cerda , Manon Laithier , Anne Baudouin , Christophe Marquette , Emma Petiot

Three-dimensional (3D) bioprinting presents a transformative approach to replicating vivo-like environments for mammalian cell cultures, offering potential advances in bioproduction and tissue engineering. In this study, we investigated the growth, metabolic activity, and structural organization of four mammalian cell lines (HEK, MDCK, CHO, and Vero) in 3D bioprinted constructs. Our results demonstrate that even highly selected, immortalized cell lines can regain physiological traits closer to their native tissue when cultured in 3D environments. We observed significant shifts in proliferation kinetics, including reduced growth rates and reduced fermentative activity. A Design of Experiment (DOE) approach identified critical biofabrication parameters—such as hydrogel microporosity and cross-linking conditions—that modulate cell behavior and proliferation in 3D matrices. These findings highlight the potential of 3D bioprinting not only for medical applications, such as regenerative medicine and drug testing, but also for enhancing bioproduction processes by supporting higher cell densities and metabolic efficiency. Our work underscores the importance of optimizing 3D culture conditions to mimic vivo-like behaviors and improve productivity, offering new insights into the scalability of bioprinted constructs for industrial applications.

三维（3D）生物打印提供了一种革命性的方法来复制哺乳动物细胞培养的活体环境，为生物生产和组织工程提供了潜在的进步。在这项研究中，我们研究了四种哺乳动物细胞系（HEK、MDCK、CHO和Vero）在生物3D打印结构中的生长、代谢活性和结构组织。我们的研究结果表明，即使是高度选择的、永生化的细胞系，在3D环境中培养时，也能恢复更接近其原生组织的生理特征。我们观察到增殖动力学的显著变化，包括生长速率降低和发酵活性降低。实验设计（DOE）方法确定了关键的生物制造参数，如水凝胶微孔隙度和交联条件，这些参数可以调节3D基质中的细胞行为和增殖。这些发现突出了3D生物打印的潜力，不仅可以用于医疗应用，如再生医学和药物测试，还可以通过支持更高的细胞密度和代谢效率来增强生物生产过程。我们的工作强调了优化3D培养条件以模仿活体行为和提高生产力的重要性，为工业应用的生物打印结构的可扩展性提供了新的见解。

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引用次数: 0

A collagen-based biomaterial ink for the digital light processing 3D printing of tough, dual-crosslinked hydrogels via post-print tannic acid treatment 一种基于胶原蛋白的生物材料墨水，用于通过打印后单宁酸处理的坚韧双交联水凝胶的数字光处理3D打印

Q1 Computer Science

Bioprinting

Pub Date : 2025-06-11 DOI: 10.1016/j.bprint.2025.e00422

Christopher R. Fellin , Richard Steiner , Xiaoning Yuan , Shailly H. Jariwala

Collagen-based biomaterial inks for digital light processing (DLP) 3D printing are particularly attractive due to their inherent biocompatibility, cell-adhesion properties, and biodegradability. However, there have been relatively few examples of collagen-based biomaterial inks without the use of synthetic co-monomers or specialized printing equipment. Furthermore, photo-crosslinked collagen hydrogels are often brittle, limiting their use in biomedical applications and regenerative medicine. In this study, we present the development of a novel collagen-based biomaterial ink for DLP 3D printing, enabling the fabrication of robust hydrogel constructs through a post-print tannic acid (TA) treatment. The biomaterial ink, composed of collagen methacrylate (ColMA) and a natural co-monomer, hyaluronic acid methacrylate (HAMA), achieves high-resolution printing of biomimetic structures. The post-print TA treatment (0.25–30 mg/mL) significantly increases mechanical strength, improves degradation rates, and reduces the size and porosity of the resulting dual-crosslinked, hybrid network structures. The biocompatibility of these constructs was assessed using adult human dermal fibroblasts, revealing optimal cell viability and adhesion at low TA concentrations (0–0.25 mg/mL). Furthermore, the antioxidant capacity of TA-treated biomaterials was evaluated, demonstrating potential for applications in environments with high reactive oxygen species (ROS). Overall, this collagen-based biomaterial ink and post-print TA treatment offers a promising solution for the DLP 3D printing of tough, biodegradable, and biocompatible constructs for biomedical applications in regenerative medicine.

用于数字光处理（DLP） 3D打印的胶原基生物材料墨水由于其固有的生物相容性、细胞粘附性和可生物降解性而特别具有吸引力。然而，在不使用合成共单体或专用打印设备的情况下，胶原蛋白基生物材料油墨的例子相对较少。此外，光交联胶原蛋白水凝胶往往易碎，限制了它们在生物医学和再生医学中的应用。在这项研究中，我们提出了一种用于DLP 3D打印的新型胶原基生物材料墨水的开发，通过打印后单宁酸（TA）处理，可以制造出坚固的水凝胶结构。该生物材料墨水由甲基丙烯酸胶原蛋白（ColMA）和天然共聚物甲基丙烯酸透明质酸（HAMA）组成，可实现仿生结构的高分辨率打印。打印后的TA处理（0.25-30 mg/mL）显著提高了机械强度，提高了降解率，并减小了双交联混合网络结构的尺寸和孔隙率。使用成人真皮成纤维细胞评估这些结构的生物相容性，揭示了低TA浓度（0-0.25 mg/mL）下的最佳细胞活力和粘附性。此外，对ta处理的生物材料的抗氧化能力进行了评估，证明了其在高活性氧（ROS）环境中的应用潜力。总的来说，这种基于胶原蛋白的生物材料墨水和打印后的TA处理为DLP 3D打印坚韧、可生物降解和生物相容性结构提供了一个有前途的解决方案，可用于再生医学的生物医学应用。

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引用次数: 0

3D-printing of alginate-based nanocomposite hydrogels incorporated with bioactive glass and calcium oxide nanoparticles for tissue engineering application 3d打印海藻酸盐基纳米复合水凝胶，结合生物活性玻璃和氧化钙纳米颗粒，用于组织工程应用

Q1 Computer Science

Bioprinting

Pub Date : 2025-06-10 DOI: 10.1016/j.bprint.2025.e00421

Mahsa Mohammadzadeh, Majid Goli, Kimia Eslami Shahrebabaki, Atefeh Golshirazi, Sheyda Labbaf

The current study focuses on optimizing alginate-based hydrogel ink for 3D bioprinting applications. A range of additives was utilized to enhance the properties of the alginate matrix, including pre-crosslinking treatments, varying concentrations of gelatin, and the incorporation of bioactive glass (BG) and calcium oxide (CaO) nanoparticles. Following the optimization of printing parameters, the formulation containing 7 % alginate and 2 % gelatin was selected as the control sample. Bioactive glass and calcium oxide nanoparticles were incorporated individually and in combinations at ratios of 70:30 and 50:50. These nanoparticles significantly improved the mechanical properties of the scaffolds, particularly tensile strength and elongation. Notably, the inclusion of nanoparticles in a 50:50 ratio increased the tensile strength of the scaffold from 105 kPa (control) to 185 kPa. Furthermore, the addition of nanoparticles enhanced the hydrophilicity of the scaffolds, reducing the contact angle from 63° (control) to 37° (50:50 sample), and improved cellular adhesion. The evaluation of cellular viability demonstrated a survival rate of 90 % for scaffolds with incorporated nanoparticles. Antibacterial tests revealed substantial effectiveness against Escherichia coli, whereas Staphylococcus aureus showed higher resistance. Overall, the findings indicate that alginate-based scaffolds, particularly those incorporating a 50:50 blend of BG and CaO with gelatin, achieve a favorable balance of mechanical performance, biocompatibility, and antibacterial properties, making them promising candidates for tissue engineering applications.

目前的研究重点是优化海藻酸盐基水凝胶墨水的3D生物打印应用。研究人员使用了一系列添加剂来增强海藻酸盐基质的性能，包括预交联处理、不同浓度的明胶、生物活性玻璃（BG）和氧化钙（CaO）纳米颗粒的掺入。通过对印刷工艺参数的优化，选择含有7%海藻酸盐和2%明胶的配方作为对照样品。生物活性玻璃和氧化钙纳米颗粒分别以70:30和50:50的比例组合加入。这些纳米颗粒显著提高了支架的机械性能，特别是抗拉强度和伸长率。值得注意的是，以50:50的比例加入纳米颗粒使支架的抗拉强度从105 kPa（对照组）增加到185 kPa。此外，纳米颗粒的加入增强了支架的亲水性，将接触角从63°（对照）降低到37°（50:50样品），并改善了细胞粘附性。细胞活力评估表明，加入纳米颗粒的支架的存活率为90%。抗菌试验显示对大肠杆菌有显著的效果，而对金黄色葡萄球菌有较高的耐药性。总的来说，研究结果表明，海藻酸盐基支架，特别是那些将BG和CaO与明胶50:50混合的支架，在机械性能、生物相容性和抗菌性能方面取得了良好的平衡，使其成为组织工程应用的有希望的候选者。

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引用次数: 0

Bioactivity, mineralization, and mechanical properties of 3D-printed nano TiO2-reinforced polymer composite immersed in SBF 3d打印纳米tio2增强聚合物复合材料浸在SBF中的生物活性、矿化和力学性能

Q1 Computer Science

Bioprinting

Pub Date : 2025-05-31 DOI: 10.1016/j.bprint.2025.e00420

Musa Yilmaz , Derya Kapusuz Yavuz

In this work, 3D-printed polylactic acid (PLA) composites reinforced with 2 wt% nanosized titanium dioxide (TiO₂) were fabricated via fused filament fabrication (FFF) to enhance surface bioactivity and overall material performance. The incorporation of TiO₂ markedly improved the apatite-forming ability of the composite surfaces, as evidenced by increased calcium and phosphorus deposition up to 0.032 and 0.046 %, respectively. Surface roughness measurements revealed that TiO₂ addition led to smoother and more uniform 3D-printed surfaces. Mechanical testing showed ∼24 % reduction in tensile strength and ∼17 % reduction in bending force compared to unreinforced PLA-polymer, predominantly attributed to nanoparticle-induced microvoid formation; despite that, the mechanical properties remained within acceptable ranges for biomedical applications. These findings suggest that the enhanced mineralization behavior, improved surface characteristics, and satisfactory mechanical integrity of TiO₂–PLA composites render them promising candidates for load-bearing biomedical applications, such as bone fixation devices and regenerative bone scaffolds.

在这项工作中，通过熔融长丝制造（FFF）制备了2 wt%纳米二氧化钛（TiO2）增强的3d打印聚乳酸（PLA）复合材料，以提高表面生物活性和整体材料性能。TiO2的掺入显著提高了复合材料表面磷灰石的形成能力，钙和磷的沉积分别增加了0.032%和0.046%。表面粗糙度测量表明，TiO2的加入使3d打印表面更光滑、更均匀。力学测试表明，与未增强的pla聚合物相比，拉伸强度降低了~ 24%，弯曲力降低了~ 17%，这主要归因于纳米颗粒诱导的微孔形成；尽管如此，机械性能仍然在生物医学应用的可接受范围内。这些发现表明，TiO2-PLA复合材料增强的矿化行为、改善的表面特性和令人满意的机械完整性使其成为承载生物医学应用的有希望的候选者，如骨固定装置和再生骨支架。

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引用次数: 0

PVA-based bioinks for 3D bioprinting: A comprehensive review of their applications in tissue engineering 基于pva的3D生物打印生物墨水：其在组织工程中的应用综述

Q1 Computer Science

Bioprinting

Pub Date : 2025-05-20 DOI: 10.1016/j.bprint.2025.e00419

Narges Johari , Zary Adabavazeh , Francesco Baino

3D bioprinting is an innovative approach that overcomes the limitations of traditional methods for creating cell-laden biomaterials and constructs. It allows for the fabrication of complex and biologically active tissue structures. This review aims to provide a comprehensive evaluation of polyvinyl alcohol (PVA)-based bioinks within a 3D bioprinting framework. PVA-based bioinks exhibit remarkable properties, such as biocompatibility, biodegradability, and the ability to enhance cell growth and differentiation. These properties make them appropriate for many tissue engineering applications. The study evaluates the physicochemical and biological properties of PVA bioinks, including how they combine with other materials such as gelatin, chitin, chitosan, alginate, agarose, cellulose, κ-carrageenan, methacrylate, nanoparticles, mineral additives, carbon nanotubes, graphene oxide, and extracellular matrix components. Furthermore, this review evaluates the benefits of market availability and enhanced printing resolution, in addition to the challenges posed by complexity, dependency on support baths, and the risk of contamination. The objective of this review is to draw attention to the capabilities of PVA-based bioinks and provide guidelines for future research to improve the effectiveness of these materials in tissue engineering and regenerative medicine.

3D生物打印是一种创新的方法，克服了传统方法的局限性，用于创建细胞负载生物材料和结构。它允许制造复杂的和生物活性的组织结构。这篇综述的目的是在3D生物打印框架内对聚乙烯醇（PVA）为基础的生物墨水进行全面的评价。pva基生物墨水具有显著的生物相容性、生物可降解性和促进细胞生长和分化的能力。这些特性使它们适合于许多组织工程应用。该研究评估了PVA生物墨水的物理化学和生物学特性，包括它们如何与其他材料结合，如明胶、几丁质、壳聚糖、海藻酸盐、琼脂糖、纤维素、κ-卡拉胶、甲基丙烯酸酯、纳米颗粒、矿物添加剂、碳纳米管、氧化石墨烯和细胞外基质成分。此外，本文评估了市场可用性和增强打印分辨率的好处，以及复杂性、对支持浴的依赖性和污染风险带来的挑战。本文综述的目的是为了引起人们对聚乙烯醇基生物连接材料性能的关注，并为进一步研究提高聚乙烯醇基生物连接材料在组织工程和再生医学中的有效性提供指导。

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引用次数: 0

Mechanical, in vitro and in vivo characterization of 3D-printed photo crosslinking acrylic/nano ZnO biocomposites for bone tissue engineering 用于骨组织工程的3d打印光交联丙烯酸/纳米ZnO生物复合材料的力学、体外和体内表征

Q1 Computer Science

Bioprinting

Pub Date : 2025-05-01 DOI: 10.1016/j.bprint.2025.e00418

Sally AbdulHussain Kadhum, Nassier A. Nassir

Bone is a highly vascularized tissue and self-repairing organ. However, the bone tissue may not be able to heal itself, especially when the injury size is higher than the critical size of the bone defect. Bone tissue engineering (BTE) is a well-recognized and successful approach to enhancing the remodeling process of diseased bone tissue. In this study, initial attention is focused on investigating the mechanical response of nanocomposites-based scaffolds. Photo crosslinking acrylic (PCA) resin and different weight percentages of nanoparticles of zinc oxide (nZnO) were used to make the nanocomposites using stereolithography (SLA) as a 3D printing technology. Here, the influence of nZnO on the mechanical response of the specimens was investigated under tension, compression and bending conditions. The results of these tests suggest that samples containing 1 wt% of nZnO exhibit the highest strength values under the various loading conditions used. Porous scaffolds, with a honeycomb pore shape, were then manufactured using 1 wt% of nZnO. In vitro bioactivity, in vivo biocompatibility, FTIR analysis, scanning electron microscope (SEM) morphological, X-ray radiological, and histopathological analysis were performed. Finally, it is suggested that the high osteogenesis of the 3D-printed porous scaffolds investigated makes it a promising and effective candidate for bone infection treatment.

骨是一个高度血管化的组织和自我修复的器官。然而，骨组织可能无法自我愈合，特别是当损伤尺寸高于骨缺损的临界尺寸时。骨组织工程（Bone tissue engineering， BTE）是一种被广泛认可和成功的增强病变骨组织重塑过程的方法。在这项研究中，最初的注意力集中在研究纳米复合材料支架的力学响应上。以光交联丙烯酸树脂（PCA）和不同重量百分比的纳米氧化锌（nZnO）为原料，采用立体光刻（SLA） 3D打印技术制备纳米复合材料。研究了nZnO在拉伸、压缩和弯曲条件下对试件力学响应的影响。这些试验结果表明，在各种加载条件下，含有1wt % nZnO的样品表现出最高的强度值。多孔支架，具有蜂窝孔形状，然后用1wt %的nZnO制造。体外生物活性、体内生物相容性、FTIR分析、扫描电镜（SEM）形态学、x线放射学和组织病理学分析。最后，我们认为所研究的3d打印多孔支架的高成骨性使其成为治疗骨感染的一个有希望和有效的候选材料。

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