The promise of bioprinting in diverse applications ranging from in vitro drug screening to creating patient-specific tissues/organs for personalized medicine has attracted significant attention globally. In this context, this review discusses the progress being made over the last decades with gelatin methacryloyl (GelMA) as a foundational hydrogel for diverse bioprintable ink formulations in particular relevance to printability, buildability, and bio-functionality. Furthermore, a comprehensive analysis is presented on the recent developments in 3D (bio)printing of GelMA for the reconstruction or regeneration of artificial tissues, spanning musculoskeletal, neurological, cardiovascular, urological, ophthalmological, dermatological, and drug screening of cancer-related applications. While presenting such wide-ranging potential, an emphasis is placed on addressing the key challenges associated with scaling up from small-scale laboratory practices to clinical applications. Furthermore, the review sheds light on the regulatory framework-related issues that impede the widespread clinical usage of 3D bioprinted tissues and organs in patient care. Taken together, this review provides significant insights into the current state of the field for researchers, clinicians, and policymakers, while navigating the intricate landscape of 3D (bio)printing for clinical translation.
{"title":"3D bioprinted GelMA scaffolds for clinical applications: Promise and challenges","authors":"Soumitra Das, Remya Valoor, Jeyapriya Thimukonda Jegadeesan, Bikramjit Basu","doi":"10.1016/j.bprint.2024.e00365","DOIUrl":"10.1016/j.bprint.2024.e00365","url":null,"abstract":"<div><div>The promise of bioprinting in diverse applications ranging from <em>in vitro</em> drug screening to creating patient-specific tissues/organs for personalized medicine has attracted significant attention globally. In this context, this review discusses the progress being made over the last decades with gelatin methacryloyl (GelMA) as a foundational hydrogel for diverse bioprintable ink formulations in particular relevance to printability, buildability, and bio-functionality. Furthermore, a comprehensive analysis is presented on the recent developments in 3D (bio)printing of GelMA for the reconstruction or regeneration of artificial tissues, spanning musculoskeletal, neurological, cardiovascular, urological, ophthalmological, dermatological, and drug screening of cancer-related applications. While presenting such wide-ranging potential, an emphasis is placed on addressing the key challenges associated with scaling up from small-scale laboratory practices to clinical applications. Furthermore, the review sheds light on the regulatory framework-related issues that impede the widespread clinical usage of 3D bioprinted tissues and organs in patient care. Taken together, this review provides significant insights into the current state of the field for researchers, clinicians, and policymakers, while navigating the intricate landscape of 3D (bio)printing for clinical translation.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00365"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Synovial joints, and particularly the osteochondral unit, are prone to lesions, with high risk of degeneration towards osteoarthritis. Various treatment strategies have been developed, including surgical techniques and cellular therapies, but they all show limitations. In this context, tissue engineering approaches, particularly 3D bioprinting, are promising for generating osteochondral tissue substitutes for joint repair. In this work, two biofabrication techniques, casting and extrusion-based 3D bioprinting, of an optimized formulation of a gelatin/alginate/fibrinogen bioink loaded with murine mesenchymal stromal cells (MSCs) were compared for the generation of cartilage and bone substitutes. Cell viability, proliferation and differentiation were characterized. Both techniques showed similar results in terms of viability and proliferation, but only the 3D bioprinted constructs allowed for differentiation towards the chondrogenic or osteogenic lineage using specific culture media. Bioprinting of biphasic osteochondral constructs comprising a cartilage compartment on top of a bone compartment was also explored. The study highlights the potential of our natural composite hydrogel bioink and extrusion-based 3D bioprinting for the generation of osteochondral tissue substitutes. Although further optimizations are needed, the study laid the groundwork for future advancements in osteochondral tissue engineering.
{"title":"A natural composite hydrogel laden with mesenchymal stromal cells for osteochondral repair: Comparison between casting and 3D bioprinting","authors":"Marjorie Dufaud , Christophe Marquette , Christian Jorgensen , Emeline Perrier-Groult , Danièle Noël","doi":"10.1016/j.bprint.2024.e00366","DOIUrl":"10.1016/j.bprint.2024.e00366","url":null,"abstract":"<div><div>Synovial joints, and particularly the osteochondral unit, are prone to lesions, with high risk of degeneration towards osteoarthritis. Various treatment strategies have been developed, including surgical techniques and cellular therapies, but they all show limitations. In this context, tissue engineering approaches, particularly 3D bioprinting, are promising for generating osteochondral tissue substitutes for joint repair. In this work, two biofabrication techniques, casting and extrusion-based 3D bioprinting, of an optimized formulation of a gelatin/alginate/fibrinogen bioink loaded with murine mesenchymal stromal cells (MSCs) were compared for the generation of cartilage and bone substitutes. Cell viability, proliferation and differentiation were characterized. Both techniques showed similar results in terms of viability and proliferation, but only the 3D bioprinted constructs allowed for differentiation towards the chondrogenic or osteogenic lineage using specific culture media. Bioprinting of biphasic osteochondral constructs comprising a cartilage compartment on top of a bone compartment was also explored. The study highlights the potential of our natural composite hydrogel bioink and extrusion-based 3D bioprinting for the generation of osteochondral tissue substitutes. Although further optimizations are needed, the study laid the groundwork for future advancements in osteochondral tissue engineering.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00366"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The most common reason for death worldwide is cardiovascular problems, and current treatments including medication, surgery, and heart transplants have disadvantages. Both 3D and 4D printing technologies are being investigated due to the demand for sophisticated solutions in cardiac care. With the use of these technologies, it may be possible to construct intricate circulatory systems, provide individualized care, and find solutions to problems like organ shortages and immune rejection. The paper focuses on various bioprinting methods that may be used in cardiac tissue engineering to create biomimetic structures, improve vascularization, and construct functional heart tissues using 3D and 4D manufacturing. The advancement of 3D and 4D printing procedures has led to substantial advancements in heart tissue engineering by offering precise and customized solutions. These technologies make it possible to fabricate intricate cardiovascular models along with medical equipment, which improves surgical planning and allows for patient-specific therapies. There are still challenges to be solved, primarily in the areas of realistic vascularization and the use of biomaterials that resemble natural cardiac tissue in terms of their mechanical and chemical properties. Technologies for 3D and 4D printing hold promise for resolving major issues with heart transplantation, namely donor scarcity and rejection. Improving vascularization along with biomaterial incorporation for therapeutic applications has to be the main goal of future research.
{"title":"3D and 4D printed materials for cardiac transplantation: Advances in biogenerative engineering","authors":"Aayush Prakash , Sathvik Belagodu Sridhar , Adil Farooq Wali , Sirajunisa Talath , Javedh Shareef , Rishabha Malviya","doi":"10.1016/j.bprint.2024.e00362","DOIUrl":"10.1016/j.bprint.2024.e00362","url":null,"abstract":"<div><div>The most common reason for death worldwide is cardiovascular problems, and current treatments including medication, surgery, and heart transplants have disadvantages. Both 3D and 4D printing technologies are being investigated due to the demand for sophisticated solutions in cardiac care. With the use of these technologies, it may be possible to construct intricate circulatory systems, provide individualized care, and find solutions to problems like organ shortages and immune rejection. The paper focuses on various bioprinting methods that may be used in cardiac tissue engineering to create biomimetic structures, improve vascularization, and construct functional heart tissues using 3D and 4D manufacturing. The advancement of 3D and 4D printing procedures has led to substantial advancements in heart tissue engineering by offering precise and customized solutions. These technologies make it possible to fabricate intricate cardiovascular models along with medical equipment, which improves surgical planning and allows for patient-specific therapies. There are still challenges to be solved, primarily in the areas of realistic vascularization and the use of biomaterials that resemble natural cardiac tissue in terms of their mechanical and chemical properties. Technologies for 3D and 4D printing hold promise for resolving major issues with heart transplantation, namely donor scarcity and rejection. Improving vascularization along with biomaterial incorporation for therapeutic applications has to be the main goal of future research.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00362"},"PeriodicalIF":0.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The process involved in the discovery of novel drugs in medical sciences is challenging due to the time-intensive process that results in a high cost of development. Additionally, it is reported that 90 % of new drugs fail in clinical trials and cannot reach the market. One of the primary reasons for failure is that research laboratories and pharmaceutical companies have been relying exclusively on data derived from animal-based models for testing the efficacy and safety of newly developed drugs. These models do not completely recapitulate human physiology or pathophysiology, resulting in a lower translational rate. Further, the evaluation of toxicity of drugs to the human body requires a more robust and holistic approach. Researchers across the globe are focusing on developing in vitro3D models as alternatives to traditional animal testing to circumvent these challenges. These model systems could replicate and mimic the human physiological microenvironment, cellular interactions, and arrangements. In vitro3D models would provide improved methods to evaluate and comprehend drug response, thereby reducing the burden on animal usage. Further, reducing the time and costs associated with developing, screening, drug failure, and translation of drugs is also realizable. In this communication, existing in vitro 3D models that are used in the drug development process are reviewed. In addition, the advancements in using 3D bioprinting and organ-on-a-chip technologies towards generating human reconstructed tissues/organs are also highlighted. The challenges from a technological and regulatory perspective on adapting these alternate animal models are also discussed.
在医学科学领域，发现新药的过程具有挑战性，因为时间密集，开发成本高昂。此外，据报道，90% 的新药在临床试验中失败，无法进入市场。失败的主要原因之一是，研究实验室和制药公司一直完全依赖动物模型得出的数据来测试新开发药物的疗效和安全性。这些模型并不能完全再现人体生理或病理生理学，导致转化率较低。此外，评估药物对人体的毒性需要更强大、更全面的方法。全球研究人员正致力于开发体外 3D 模型，以替代传统的动物试验，从而规避这些挑战。这些模型系统可以复制和模拟人体生理微环境、细胞相互作用和排列。体外三维模型将为评估和理解药物反应提供更好的方法，从而减轻使用动物的负担。此外，减少与药物开发、筛选、药物失效和转化相关的时间和成本也是可以实现的。本文回顾了药物开发过程中使用的现有体外三维模型。此外，还重点介绍了利用三维生物打印和芯片器官技术生成人体重建组织/器官的进展。此外，还从技术和监管的角度讨论了采用这些替代动物模型所面临的挑战。
{"title":"Evolution of toxicity testing platforms from 2D to advanced 3D bioprinting for safety assessment of drugs","authors":"Rohin Shyam , Rinni Singh , Mukul Bajpai , Arunkumar Palaniappan , Ramakrishnan Parthasarathi","doi":"10.1016/j.bprint.2024.e00363","DOIUrl":"10.1016/j.bprint.2024.e00363","url":null,"abstract":"<div><div>The process involved in the discovery of novel drugs in medical sciences is challenging due to the time-intensive process that results in a high cost of development. Additionally, it is reported that 90 % of new drugs fail in clinical trials and cannot reach the market. One of the primary reasons for failure is that research laboratories and pharmaceutical companies have been relying exclusively on data derived from animal-based models for testing the efficacy and safety of newly developed drugs. These models do not completely recapitulate human physiology or pathophysiology, resulting in a lower translational rate. Further, the evaluation of toxicity of drugs to the human body requires a more robust and holistic approach. Researchers across the globe are focusing on developing <em>in vitro</em>3D models as alternatives to traditional animal testing to circumvent these challenges. These model systems could replicate and mimic the human physiological microenvironment, cellular interactions, and arrangements. <em>In vitro</em>3D models would provide improved methods to evaluate and comprehend drug response, thereby reducing the burden on animal usage. Further, reducing the time and costs associated with developing, screening, drug failure, and translation of drugs is also realizable. In this communication, existing <em>in vitro</em> 3D models that are used in the drug development process are reviewed. In addition, the advancements in using 3D bioprinting and organ-on-a-chip technologies towards generating human reconstructed tissues/organs are also highlighted. The challenges from a technological and regulatory perspective on adapting these alternate animal models are also discussed.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00363"},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polycaprolactone (PCL), either in its pure grade or as a polymeric matrix for bio-composites, plays a key role in the biomedical and bioengineering industries. It is also considered a multifunctional and versatile polymer for bioprinting and bioplotting purposes, especially in tissue engineering. Herein, an undiscovered yet valuable aspect of PCL extrusion-based bioprinting, such as the predictability of Critical Quality Indicators (CQIs), is investigated in depth. With the aid of the robust L25 orthogonal matrix design, the six most generic and device-independent control factors proved their impact on quality metrics such as global porosity, dimensional conformity, and surface roughness, determined with the aid of highly evolved Nondestructive Testing (NDT) and algorithms. To this end, 25 experimental runs were set, and 125 specimens were fabricated using an industrial-scale bio-plotter and medical-graded polycaprolactone. Various infill densities (ID), layer thicknesses (LT), raster deposition angles (RDA), printing speeds (PS), nozzle temperatures (NT), and bed temperatures (BT) were applied. CQIs were determined using optical profilometry and microscopy, and micro-computed tomography. Quadratic predictive equations were compiled and verified using two additional, well-chosen experimental runs. These generally applicable predictive models carry a massive amount of research and industrial merit, as they ensure visibility in bioprinting with PCL.
{"title":"Robust design optimization of Critical Quality Indicators (CQIs) of medical-graded polycaprolactone (PCL) in bioplotting","authors":"Nectarios Vidakis , Markos Petousis , Constantine David , Dimitrios Sagris , Nikolaos Mountakis , Mariza Spiridaki , Amalia Moutsopoulou , Nektarios K. Nasikas","doi":"10.1016/j.bprint.2024.e00361","DOIUrl":"10.1016/j.bprint.2024.e00361","url":null,"abstract":"<div><div>Polycaprolactone (PCL), either in its pure grade or as a polymeric matrix for bio-composites, plays a key role in the biomedical and bioengineering industries. It is also considered a multifunctional and versatile polymer for bioprinting and bioplotting purposes, especially in tissue engineering. Herein, an undiscovered yet valuable aspect of PCL extrusion-based bioprinting, such as the predictability of Critical Quality Indicators (CQIs), is investigated in depth. With the aid of the robust L25 orthogonal matrix design, the six most generic and device-independent control factors proved their impact on quality metrics such as global porosity, dimensional conformity, and surface roughness, determined with the aid of highly evolved Nondestructive Testing (NDT) and algorithms. To this end, 25 experimental runs were set, and 125 specimens were fabricated using an industrial-scale bio-plotter and medical-graded polycaprolactone. Various infill densities (ID), layer thicknesses (LT), raster deposition angles (RDA), printing speeds (PS), nozzle temperatures (NT), and bed temperatures (BT) were applied. CQIs were determined using optical profilometry and microscopy, and micro-computed tomography. Quadratic predictive equations were compiled and verified using two additional, well-chosen experimental runs. These generally applicable predictive models carry a massive amount of research and industrial merit, as they ensure visibility in bioprinting with PCL.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00361"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.bprint.2024.e00360
Ambreen Afridi, Ans Al Rashid, Muammer Koç
Additive manufacturing processes have progressed over recent years due to their superiority over conventional manufacturing methods. Their ability to fabricate materials with complex structures, increased precision, and reduced cost have opened avenues for various industrial applications, including biomedical, electrical, mechanical, aviation, and filtration, and led to their development over time. Stereolithography (SLA) is an additive manufacturing technique, through photopolymerization reaction, it solidifies a selective resin to produce three-dimensional objects. SLA has emerged as a leading 3D printing technique, revolutionizing prototyping and production across various industries. SLA has been through four generations of development and advancement, resulting in its improved performance, the diversity of materials, and the variety of applications. Stereolithography has diversified its material and emerged as a promising method for polymer-based composite when operating under optimized conditions. SLA offers superior resolution, high finish quality, improved speed and precision, and is cost-effective compared to alternative techniques like Fused Deposition Modeling (FDM). This current study aims to comprehensively review SLA development, its processes, applications and inherent challenges in mechanical, electrical and biomedical fields.
近年来，快速成型制造工艺因其优于传统制造方法而不断进步。它们能够制造结构复杂的材料，提高精度，降低成本，为生物医学、电气、机械、航空和过滤等各种工业应用开辟了道路，并促使其不断发展。立体光刻（SLA）是一种增材制造技术，它通过光聚合反应，固化选择性树脂来制造三维物体。SLA 已成为一种领先的 3D 打印技术，为各行各业的原型设计和生产带来了革命性的变化。立体光刻技术经历了四代的发展和进步，其性能不断提高，材料更加多样化，应用领域也更加广泛。立体光刻技术实现了材料的多样化，并在优化条件下成为聚合物基复合材料的一种有前途的方法。与熔融沉积建模（FDM）等替代技术相比，SLA 具有分辨率高、光洁度高、速度快、精度高、成本低等优点。本研究旨在全面回顾 SLA 的发展及其在机械、电气和生物医学领域的工艺、应用和固有挑战。
{"title":"Recent advances in the development of stereolithography-based additive manufacturing processes: A review of applications and challenges","authors":"Ambreen Afridi, Ans Al Rashid, Muammer Koç","doi":"10.1016/j.bprint.2024.e00360","DOIUrl":"10.1016/j.bprint.2024.e00360","url":null,"abstract":"<div><div>Additive manufacturing processes have progressed over recent years due to their superiority over conventional manufacturing methods. Their ability to fabricate materials with complex structures, increased precision, and reduced cost have opened avenues for various industrial applications, including biomedical, electrical, mechanical, aviation, and filtration, and led to their development over time. Stereolithography (SLA) is an additive manufacturing technique, through photopolymerization reaction, it solidifies a selective resin to produce three-dimensional objects. SLA has emerged as a leading 3D printing technique, revolutionizing prototyping and production across various industries. SLA has been through four generations of development and advancement, resulting in its improved performance, the diversity of materials, and the variety of applications. Stereolithography has diversified its material and emerged as a promising method for polymer-based composite when operating under optimized conditions. SLA offers superior resolution, high finish quality, improved speed and precision, and is cost-effective compared to alternative techniques like Fused Deposition Modeling (FDM). This current study aims to comprehensively review SLA development, its processes, applications and inherent challenges in mechanical, electrical and biomedical fields.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00360"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.1016/j.bprint.2024.e00358
Ingri Julieth Mancilla Corzo , Jessica Heline Lopes da Fonseca , Victor Ferman , Diego Noé Rodríguez Sánchez , Alexandre Leite Rodrigues de Oliveira , Marcos Akira d'Ávila
Tissue engineering approaches require biocompatible materials with precise pre-designed geometry, shape fidelity, and promote cellular functions. Addressing these requirements, our study focused on developing an optimized bioink formulation using carboxymethyl cellulose (CMC) and Laponite hydrogels tailored for extrusion-based three-dimensional bioprinting. To this, we investigated the rheological properties and filament behavior before and during printing. As Laponite concentration increased in CMC solutions, it improved shear-thinning behavior, viscosity, and storage modulus, resulting in well-defined filament characteristics with lower diffusion rates, excellent shape fidelity, and robust printability. Thus, we achieved a suitable biomaterial ink formulation with concentrations of 1 wt% of CMC and 4 wt% of Laponite (1C4L). Subsequently, a statistical analysis guided us to select the optimal parameters for large-scale construct printing: a nozzle speed of 5 mm/s, a print distance of 0.41 mm, and an extrusion multiplier of 1.35. After that, we enhanced the structural integrity of printed hydrogels through ionic crosslinking with calcium chloride (CaCl2) and citric acid (CA), revealing higher-strength hydrogels at higher concentrations of CaCl2. Finally, we have confirmed the groundbreaking potential of our bioink by integrating dental pulp mesenchymal stem cells (DPSC) into the 1C4L ink. Our bioprinted constructs showed optimized swelling, non-toxic effects, and retained excellent shape fidelity, crucial for creating anatomically accurate tissues. Our findings provide crucial insights linking the rheological analysis, the bioprinting process, and the biological properties of hydrogels, paving the way for their use for tissue engineering and other biomedical applications.
{"title":"Optimizing biomaterial inks: A study on the printability of Carboxymethyl cellulose-Laponite nanocomposite hydrogels and dental pulp stem cells bioprinting","authors":"Ingri Julieth Mancilla Corzo , Jessica Heline Lopes da Fonseca , Victor Ferman , Diego Noé Rodríguez Sánchez , Alexandre Leite Rodrigues de Oliveira , Marcos Akira d'Ávila","doi":"10.1016/j.bprint.2024.e00358","DOIUrl":"10.1016/j.bprint.2024.e00358","url":null,"abstract":"<div><p>Tissue engineering approaches require biocompatible materials with precise pre-designed geometry, shape fidelity, and promote cellular functions. Addressing these requirements, our study focused on developing an optimized bioink formulation using carboxymethyl cellulose (CMC) and Laponite hydrogels tailored for extrusion-based three-dimensional bioprinting. To this, we investigated the rheological properties and filament behavior before and during printing. As Laponite concentration increased in CMC solutions, it improved shear-thinning behavior, viscosity, and storage modulus, resulting in well-defined filament characteristics with lower diffusion rates, excellent shape fidelity, and robust printability. Thus, we achieved a suitable biomaterial ink formulation with concentrations of 1 wt% of CMC and 4 wt% of Laponite (1C4L). Subsequently, a statistical analysis guided us to select the optimal parameters for large-scale construct printing: a nozzle speed of 5 mm/s, a print distance of 0.41 mm, and an extrusion multiplier of 1.35. After that, we enhanced the structural integrity of printed hydrogels through ionic crosslinking with calcium chloride (CaCl<sub>2</sub>) and citric acid (CA), revealing higher-strength hydrogels at higher concentrations of CaCl<sub>2</sub>. Finally, we have confirmed the groundbreaking potential of our bioink by integrating dental pulp mesenchymal stem cells (DPSC) into the 1C4L ink. Our bioprinted constructs showed optimized swelling, non-toxic effects, and retained excellent shape fidelity, crucial for creating anatomically accurate tissues. Our findings provide crucial insights linking the rheological analysis, the bioprinting process, and the biological properties of hydrogels, paving the way for their use for tissue engineering and other biomedical applications.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00358"},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.1016/j.bprint.2024.e00359
Larissa R. Lourenço , Roger Borges , Danilo Carastan , Mônica B. Mathor , Juliana Marchi
3D printing technology in tissue engineering applications provides several advantages for scaffold development, especially with natural materials, such as chitosan, which provides a biomimetic environment for cellular growth. However, chitosan hydrogel-based inks still show poor printing fidelity. In this article, we overcame this challenge by incorporating bioactive glasses (BG) nanoparticles (up to 5 wt%) into the chitosan hydrogel. The resulting inks were characterized by rheological tests, while their processability was evaluated through measurements of shape fidelity. An indirect cytotoxicity assay was also conducted to evaluate the cell viability of the printed scaffolds. The results indicated that adding BG nanoparticles to the chitosan-based ink modified its rheological properties and improved its shape-fidelity during 3D printing, which we suggest are consequences of hydrogen bonds established between the glass and the chitosan chains. Also, cytotoxicity assessment demonstrated that the resulting scaffold exhibits high cell viability. In conclusion, the proposed composite ink has optimized rheological properties for 3D printing and is promising for applications in tissue engineering.
组织工程应用中的三维打印技术为支架开发提供了多种优势，尤其是天然材料，如壳聚糖，它能为细胞生长提供仿生环境。然而，基于壳聚糖水凝胶的油墨仍然显示出较低的打印保真度。在本文中，我们通过在壳聚糖水凝胶中加入生物活性玻璃 (BG) 纳米颗粒（最多 5 wt%）来克服这一难题。我们通过流变学测试对所得油墨进行了表征，并通过形状保真度测量对其加工性进行了评估。此外，还进行了间接细胞毒性试验，以评估印刷支架的细胞活力。结果表明，在壳聚糖基墨水中添加 BG 纳米粒子可改变其流变特性，并改善其在 3D 打印过程中的形状保真度，我们认为这是玻璃和壳聚糖链之间建立氢键的结果。此外，细胞毒性评估表明，生成的支架具有很高的细胞活力。总之，所提出的复合油墨具有优化的三维打印流变特性，有望应用于组织工程。
{"title":"Precision 3D printing of chitosan-bioactive glass inks: Rheological optimization for enhanced shape fidelity in tissue engineering scaffolds","authors":"Larissa R. Lourenço , Roger Borges , Danilo Carastan , Mônica B. Mathor , Juliana Marchi","doi":"10.1016/j.bprint.2024.e00359","DOIUrl":"10.1016/j.bprint.2024.e00359","url":null,"abstract":"<div><div>3D printing technology in tissue engineering applications provides several advantages for scaffold development, especially with natural materials, such as chitosan, which provides a biomimetic environment for cellular growth. However, chitosan hydrogel-based inks still show poor printing fidelity. In this article, we overcame this challenge by incorporating bioactive glasses (BG) nanoparticles (up to 5 wt%) into the chitosan hydrogel. The resulting inks were characterized by rheological tests, while their processability was evaluated through measurements of shape fidelity. An indirect cytotoxicity assay was also conducted to evaluate the cell viability of the printed scaffolds. The results indicated that adding BG nanoparticles to the chitosan-based ink modified its rheological properties and improved its shape-fidelity during 3D printing, which we suggest are consequences of hydrogen bonds established between the glass and the chitosan chains. Also, cytotoxicity assessment demonstrated that the resulting scaffold exhibits high cell viability. In conclusion, the proposed composite ink has optimized rheological properties for 3D printing and is promising for applications in tissue engineering.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00359"},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-13DOI: 10.1016/j.bprint.2024.e00357
Swayam Aryam Behera, Binita Nanda, P. Ganga Raju Achary
3D bioprinting has emerged as a promising technology with transformative potential in cancer research and therapy. This review explores the innovative applications, challenges, and future directions of 3D bioprinting in the field of cancer. By recapitulating tumor microenvironments and heterogeneity, 3D bioprinted models offer valuable platforms for studying cancer biology, drug responses, and personalized medicine. The integration of 3D bioprinting with other cutting-edge technologies, such as organ-on-a-chip and microfluidics, has further enhanced the ability to replicate the dynamic and heterogeneous nature of tumors. The forthcoming paths include advancements in biomaterial engineering, bioprinting techniques, and interdisciplinary collaborations to overcome these challenges. Integration of 3D bioprinting into clinical practice holds promise for revolutionizing cancer diagnosis, treatment, and management.
{"title":"Recent advancements and challenges in 3D bioprinting for cancer applications","authors":"Swayam Aryam Behera, Binita Nanda, P. Ganga Raju Achary","doi":"10.1016/j.bprint.2024.e00357","DOIUrl":"10.1016/j.bprint.2024.e00357","url":null,"abstract":"<div><p>3D bioprinting has emerged as a promising technology with transformative potential in cancer research and therapy. This review explores the innovative applications, challenges, and future directions of 3D bioprinting in the field of cancer. By recapitulating tumor microenvironments and heterogeneity, 3D bioprinted models offer valuable platforms for studying cancer biology, drug responses, and personalized medicine. The integration of 3D bioprinting with other cutting-edge technologies, such as organ-on-a-chip and microfluidics, has further enhanced the ability to replicate the dynamic and heterogeneous nature of tumors. The forthcoming paths include advancements in biomaterial engineering, bioprinting techniques, and interdisciplinary collaborations to overcome these challenges. Integration of 3D bioprinting into clinical practice holds promise for revolutionizing cancer diagnosis, treatment, and management.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00357"},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-24DOI: 10.1016/j.bprint.2024.e00356
Ahmed Yaseen Alqutaibi , Mohammed Ahmed Alghauli , Marwan Hamed Awad Aljohani , Muhammad Sohail Zafar
The utilization of 3D printing technologies is extensively pervasive across diverse sectors, including design, engineering, and manufacturing. These sophisticated manufacturing techniques depend on digitally designed models to autonomously construct 3D objects. With the growing interest in 3D printing within dentistry, specifically regarding dental implants, there has been a rapid dissemination of information pertaining to this domain and its applications. As a result, it has become crucial to conduct a comprehensive review on this topic. 3D printing technologies have played a pivotal role in oral implantology. This review provides a comprehensive analysis of the current state and future needs of 3D printing in implant dentistry, covering technologies, printable materials, and applications in both the surgical and prosthodontic stages of dental implant therapy. Furthermore, it discusses considerations for choosing the appropriate 3D printing technology for specific dental applications. This comprehensive examination offers key insights into the progress, practical uses, and future prospects of 3D printing in dental implants.
三维打印技术的应用广泛渗透到设计、工程和制造等各个领域。这些复杂的制造技术依赖于数字化设计的模型来自主构建三维物体。随着牙科领域对 3D 打印技术的兴趣日益浓厚，特别是在牙科植入物方面，与这一领域及其应用相关的信息得到了迅速传播。因此，对这一主题进行全面回顾变得至关重要。3D 打印技术在口腔种植学中发挥了举足轻重的作用。本综述全面分析了 3D 打印技术在种植牙领域的现状和未来需求，涵盖了种植牙治疗手术和修复阶段的技术、可打印材料和应用。此外，它还讨论了为特定牙科应用选择适当 3D 打印技术的注意事项。这本全面的研究报告提供了有关 3D 打印技术在牙科种植方面的进展、实际应用和未来前景的重要见解。
{"title":"Advanced additive manufacturing in implant dentistry: 3D printing technologies, printable materials, current applications and future requirements","authors":"Ahmed Yaseen Alqutaibi , Mohammed Ahmed Alghauli , Marwan Hamed Awad Aljohani , Muhammad Sohail Zafar","doi":"10.1016/j.bprint.2024.e00356","DOIUrl":"10.1016/j.bprint.2024.e00356","url":null,"abstract":"<div><p>The utilization of 3D printing technologies is extensively pervasive across diverse sectors, including design, engineering, and manufacturing. These sophisticated manufacturing techniques depend on digitally designed models to autonomously construct 3D objects. With the growing interest in 3D printing within dentistry, specifically regarding dental implants, there has been a rapid dissemination of information pertaining to this domain and its applications. As a result, it has become crucial to conduct a comprehensive review on this topic. 3D printing technologies have played a pivotal role in oral implantology. This review provides a comprehensive analysis of the current state and future needs of 3D printing in implant dentistry, covering technologies, printable materials, and applications in both the surgical and prosthodontic stages of dental implant therapy. Furthermore, it discusses considerations for choosing the appropriate 3D printing technology for specific dental applications. This comprehensive examination offers key insights into the progress, practical uses, and future prospects of 3D printing in dental implants.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"42 ","pages":"Article e00356"},"PeriodicalIF":0.0,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405886624000289/pdfft?md5=5604ceec5d3673820740d64f67eac60d&pid=1-s2.0-S2405886624000289-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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