Bioprinting最新文献

{"title":"Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application","authors":"Pekik Wiji Prasetyaningrum,&nbsp;Wildan Mubarok,&nbsp;Takashi Kotani,&nbsp;Shinji Sakai","doi":"10.1016/j.bprint.2025.e00439","DOIUrl":"10.1016/j.bprint.2025.e00439","url":null,"abstract":"<div><div>The use of support materials is crucial for the 3D bioprinting of low-viscosity bioinks, which yield soft hydrogel constructs susceptible to deformation under their weight. In this study, we developed a starch-based support material that provides structural support during printing and supplies hydrogen peroxide (H₂O₂), for printing cell-laden constructs from low-viscosity bioinks (4.4–53.1 mPa s at 1 s⁻¹ shear rate) composed of a gelatin derivative possessing phenolic hydroxyl moieties (gelatin-Ph), horseradish peroxidase (HRP), and cells. Importantly, the support material can be selectively and gently removed using α-amylase, a biocompatible enzyme, without harming the construct or encapsulated cells, which is a significant advancement over conventional methods of removing support systems. 3D constructs were fabricated by alternately extruding bioinks containing 5.0 w/v% gelatin-Ph and 10 U/mL HRP with a support material consisting of 16.7 w/w% starch and 10 mM H₂O₂. Immortalized human bone marrow-derived mesenchymal stem cells encapsulated within the constructs showed &gt;80 % viability after printing and exhibited an elongated morphology and proliferation, while maintaining their stemness over 14 days of culture. The cells underwent osteogenic differentiation when cultured in a differentiation medium, as evidenced by the calcium deposition, alkaline phosphatase activity, and expression of osteogenic genes, demonstrating the potential of the proposed approach for tissue-engineering applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"51 ","pages":"Article e00439"},"PeriodicalIF":0.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145098084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in polymeric nanoparticles and hydrogels in 3D bioprinting: Enhancing bioinks for tissue engineering and regenerative medicine","authors":"B. Pavithra ,&nbsp;Prabhakar Singh ,&nbsp;V Ramesh Kumar ,&nbsp;Siva Durairaj ,&nbsp;Saqib Hassan","doi":"10.1016/j.bprint.2025.e00438","DOIUrl":"10.1016/j.bprint.2025.e00438","url":null,"abstract":"<div><div>In tissue engineering and regenerative medicine, 3D bioprinting has become a revolutionary technique that makes it possible to precisely fabricate intricate biological structures. The creation of sophisticated bioinks, especially those that include hydrogels and polymeric nanoparticles, is essential to its success. These substances promote cellular adhesion, proliferation, and differentiation by providing special physicochemical characteristics that closely resemble the natural extracellular matrix. Hydrogels offer a moist, friendly environment that promotes tissue growth, whereas polymeric nanoparticles improve the mechanical strength, printability, and controlled drug administration of bioinks. Recent developments in the creation and use of hydrogels and polymeric nanoparticles in 3D bioprinting are summarized in this review, with an emphasis on their applications in organ regeneration, wound healing, and personalized medicine<strong>.</strong> It also discusses current problems that need to be resolved in order to transform laboratory breakthroughs into clinical treatments, such as biocompatibility, structural fidelity, and standardization. The future of 3D bioprinting holds the possibility of previously unheard-of advances in functional tissue restoration and patient-specific treatment through the integration of nanotechnology, machine learning, and biomaterial science.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"51 ","pages":"Article e00438"},"PeriodicalIF":0.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic study of high-performance hydroxyapatite processed by vat photopolymerization additive manufacturing","authors":"Haowen Liang ,&nbsp;Tengbo Li ,&nbsp;Junpeng Huang ,&nbsp;Binbin Guo ,&nbsp;Sijing Li ,&nbsp;Xiaoteng Chen ,&nbsp;Shixiang Yu ,&nbsp;Cheng Liu ,&nbsp;Guoxian Pei ,&nbsp;Jiaming Bai","doi":"10.1016/j.bprint.2025.e00434","DOIUrl":"10.1016/j.bprint.2025.e00434","url":null,"abstract":"<div><div>Vat photopolymerization (VPP) enables the fabrication of hydroxyapatite (HAp) with high resolution, complex geometry and interconnected porous structures. However, the inherent property characterization of the VPP-printed HAp as a comparative benchmark for peer studies is still lacking. This study systematically analyzed the performance of VPP-printed HAp with a 55 vol% solid loading, focusing on printability, fabrication quality, mechanical performance limits, reliability, and biological response. The optimized HAp slurry presented high polymerization reactivity and efficient, precise photocuring performance at 17 mJ/cm². With a high density of 98.98 % and compacted grain boundaries, the bending strength of the HAp reached 127 MPa, surpassing the highest reported value for 3D-printing HAp by 23.3 %. In vitro studies demonstrated that the VPP-printed HAp promoted osteoblast proliferation and osteogenic differentiation. The HAp fabricated via VPP with efficient printability, controllable fabrication accuracy (within 1 %) and quality, good mechanical performance and osteogenic activity showcased its promising potential in implant fabrication for bone tissue repair.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"51 ","pages":"Article e00434"},"PeriodicalIF":0.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HYBERFLOW — enabling non-invasive flow rate feedback control in bioprinting via hydraulic actuation","authors":"Leon Budde ,&nbsp;Julia Hundertmark ,&nbsp;Tim Meyer ,&nbsp;Thomas Seel ,&nbsp;Daniel O.M. Weber","doi":"10.1016/j.bprint.2025.e00435","DOIUrl":"10.1016/j.bprint.2025.e00435","url":null,"abstract":"<div><div>Bioprinting offers transformative potential for tissue engineering by enabling the precise fabrication of complex tissue constructs. Of the different bioprinting techniques, extrusion-based bioprinting is the most common, often relying on pneumatic actuation to extrude bioinks. Changes in the viscosity of the bioink, e.g., due to inhomogeneities in the ink or temperature changes in the printing environment, affect the extrusion flow rate if the pneumatic pressure is not adapted accordingly. While maintaining a constant flow rate improves the printing results significantly, continuous monitoring of the flow rate in combination with feedback control is required. Current systems rely on a flow rate sensor to directly measure the flow rate of the bioink, which negatively affects the bioink and requires frequent re-calibrations. To overcome these issues, we are using a hydraulic actuation fluid and implementing a flow rate feedback control based on the flow rate of the actuation fluid rather than the bioink itself. We integrated this concept of hydraulic actuation into our novel <strong>hy</strong>draulic <strong>b</strong>io<strong>e</strong>xtruder with <strong>r</strong>eal-time <strong>flow</strong> rate control called ”HYBERFLOW”. In this paper, we briefly present the design and our experimental validation of the system. Our experiments are aimed to determine whether the flow rate of the actuation fluid corresponds to the flow rate of the extrusion material, investigate the capabilities of the HYBERFLOW to achieve and maintain a desired flow rate with highly heterogeneous bioinks and determine the limits of the HYBERFLOW in terms of bioink viscosity and printing nozzle geometry. We found that the deviation in volume of the extruded bioink compared to the measured volume of the actuation fluid is less than 4%. This clearly shows the feasibility of controlling the flow rate of the bioink by controlling the flow rate of the actuation fluid. As a result, the flow rate sensor only needs to be in contact with actuation fluid, which is less sensitive and does not require the sensor to be re-calibrated due to its more consistent fluid properties. Furthermore, when extruding a bioink consisting of layers with different viscosities, the feedback control was able to maintain the desired flow rate, leading to a more consistent geometry of the printing result. In conclusion, HYBERFLOW enables real-time flow rate-controlled bioextrusions for improved printing outcomes without negatively affecting the bioink.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"50 ","pages":"Article e00435"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring 4D printing for biomedical applications: Advancements, challenges, and future perspectives","authors":"Ermias Wubete Fenta ,&nbsp;Ammar Alsheghri","doi":"10.1016/j.bprint.2025.e00436","DOIUrl":"10.1016/j.bprint.2025.e00436","url":null,"abstract":"<div><div>4D printing is advanced additive manufacturing (AM) technology with transforming extension of traditional 3D printing that introduces the time dimension for material manufacturing to allow printed objects to change their shape, functionality, or properties with respect to specific external stimuli such as moisture, temperature, pH, or light. 4D printing relies on smart materials such as shape memory polymers (SMPs), hydrogels, liquid crystal elastomers (LCEs), etc. It possesses tremendous scope in biomedical applications, particularly tissue engineering, drug delivery systems, orthodontics, and diagnostic devices. By adopting smart materials and exquisite fabrication techniques, smart biomedical devices developed via 4D printing reply to changes in the physiological situation, increase therapeutic effectiveness, and promote favorable treatment outcomes. This review aims to study the state of the art on 4D printing in biomedical engineering covering fundamentals, materials, applications, challenges, and future perspectives. Nevertheless, many challenges remain such as material biocompatibility, printing resolution, and precise control over transformation kinetics. Future advancements, including AI-assisted design, machine learning optimization, new smart material developments, and high-resolution printing, promise to address these challenges, accelerating the shift toward precision medicine. Collectively, 4D printing demonstrated the ability to revolutionize biosciences for patient-specific, adaptive, and minimally invasive responses to healthcare.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"50 ","pages":"Article e00436"},"PeriodicalIF":0.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-powered transformation of pharmaceutical 3D printing: enhancing precision, efficiency, and personalization","authors":"Riya Patel ,&nbsp;Shivani Patel ,&nbsp;Vanessa James ,&nbsp;Yash Raj Singh ,&nbsp;Vishruti Shah ,&nbsp;Vishvjit Thakar ,&nbsp;Bhupendra G. Prajapati","doi":"10.1016/j.bprint.2025.e00437","DOIUrl":"10.1016/j.bprint.2025.e00437","url":null,"abstract":"<div><div>Cloud computing technologies and Internet of Things systems and artificial intelligence (AI) have brought major changes to pharmaceutical 3D printing by promoting new opportunities in designing drugs and manufacturing and personalized medicine delivery. Algorithmic processing through AI improves the modeling of drugs for computation and predicts formulation stability and detects real-time defects in printed dosage forms while boosting operational efficiency. Machine learning systems help optimize printing settings to achieve consistent results and reduce material waste across production batches. The use of artificial intelligence in pharmaceutical 3D printing needs overcoming three major challenges: regulatory hurdles, standards, and data privacy concerns. To overcome these problems, regulatory authorities, pharmaceutical researchers, and technology companies must collaborate to set standards for pharmaceutical data protection as well as compliance frameworks. AI-powered software solutions employ predictive analytics to do quality control in real time, reducing the amount of manufacturing failures. This article discusses regulatory obstacles, data security issues, and standards. Furthermore, identify research gaps so that academics can continue to work on AI-based 3D printing models. The application of AI enables pharmaceutical companies to boost operational efficiency and precision capabilities as well as innovative developments that lead to advanced drug therapies adjusted for individual patients alongside contemporary production methods.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"50 ","pages":"Article e00437"},"PeriodicalIF":0.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioresorbable TPMS polymeric scaffolds for bone regeneration","authors":"Asiah Hatcher ,&nbsp;Gary Brierly ,&nbsp;Cedryck Vaquette ,&nbsp;Reuben Staples ,&nbsp;Omar Breik ,&nbsp;Sašo Ivanovski ,&nbsp;Martin D. Batstone ,&nbsp;Danilo Carluccio","doi":"10.1016/j.bprint.2025.e00433","DOIUrl":"10.1016/j.bprint.2025.e00433","url":null,"abstract":"<div><div>Bone tissue engineering (BTE) addresses limitations of traditional bone grafts by using synthetic scaffolds with or without growth factors to regenerate critical-sized defects. New generation scaffolds are produced with a biomimetic approach to simulate bone structure and support cellular functions. This review explores the potential of Triply Periodic Minimal Surface (TPMS) scaffolds made from bioresorbable polymers for BTE applications. TPMS scaffolds are designed to mimic the complex geometry of natural bone, offering a balance between mechanical strength and porosity that promotes nutrient flow and cell proliferation. This review discusses the limitations of traditional scaffold materials and fabrication methods, emphasising the advantages of additive manufacturing (AM) technologies in creating high-resolution, customisable scaffolds. The paper delves into the design principles, material choices, and clinical applications of TPMS scaffolds, with a focus on their mechanical and biological performance. It also addresses the challenges in manufacturing high-fidelity TPMS scaffolds and the need for further research to optimise their design for clinical use. The review concludes by outlining future directions for the development of TPMS scaffolds, aiming to improve their efficacy in bone regeneration and their potential for clinical translation.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"50 ","pages":"Article e00433"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-crosslinkable gallol bioinks via pH-controlled oxidation and photocrosslinking with enhanced shear thinning and viscoelastic behavior","authors":"Hatai Jongprasitkul ,&nbsp;Sanna Turunen ,&nbsp;David A. Fulton ,&nbsp;Minna Kellomäki ,&nbsp;Vijay Singh Parihar","doi":"10.1016/j.bprint.2025.e00432","DOIUrl":"10.1016/j.bprint.2025.e00432","url":null,"abstract":"<div><div>Our research work proposes a dual crosslinking approach to address the limitations of the gallol-mediated auto-oxidation approach in bioprinting, where rapid oxidative crosslinking can cause premature gelation, leading to clogging or printing failure. We enabled a gallol hydrogel ink to be printable via extrusion-based 3D bioprinting by utilizing its temporal shear-thinning properties. By raising the pH level, interactions between gallol-modified hyaluronic acid methacrylate (HAMA-GA) can be triggered to form a weak hydrogel. This feature provides injectability and extrudability for the hydrogels. Subsequent photocrosslinking results in indefinite oxidative crosslinking. The oxidative coupling in HAMA-GA was partially inhibited by UV light during the photocrosslinking step. As a result, the printed hydrogel formed a dual-crosslinked network containing both oxidative and photo-induced bonds, which contributed to enhanced structural stability over time. Our proposed approach addresses the challenges of gallol-mediated oxidation, including overgelation that hinders extrusion in 3D bioprinting, offering a promising solution for improved printability and shape fidelity. HAMA-GA ink was bioprintable at pH 5.5 using an extrusion-based 3D printer, showing cytocompatibility (∼95 % viability). This strategy is valuable for designing hydrogel inks with tunable properties for 3D bioprinting while maintaining tissue adhesive properties of gallol moieties.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"50 ","pages":"Article e00432"},"PeriodicalIF":0.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced 3D printing and multiscale technologies (nano to macro) for personalized biomedical applications","authors":"Saranya Balasubramaniyam,&nbsp;Thirumalaikumaran Rathinam,&nbsp;Mohanakrishnan Srinivasan,&nbsp;Karthikeyan Elumalai","doi":"10.1016/j.bprint.2025.e00430","DOIUrl":"10.1016/j.bprint.2025.e00430","url":null,"abstract":"<div><div>The combination of 3D printing and nanotechnology is remodeling the field of biomedical innovation, opening up unprecedented levels of precision, personalization, and function in health care. 3D printing provides the capacity to print complex, patient-specific constructs, and nanotechnology extends this with dynamic biological interactions at the molecular scale to create smart implants, responsive drug delivery devices, and regenerative tissue scaffolds. This merging not only increases mechanical and biological compatibility but also encourages the development of multifunctional devices to monitor in real time, to treat selectively, and to exhibit bio responsive behaviour. Examples ranging from 3D-bioprinted organs to nanoengineered scaffolds and smart diagnostic biosensors show the ability to solve persistent organ transplantation, cancer therapy, and chronic disease management challenges. In addition, breakthroughs such as 4D printing and AI-driven nano-bio fabrication will be pushing the boundaries further by creating patient-driven, self-adaptive therapeutic platforms. But still, large technical, regulatory, and ethical hurdles have to be crossed in order to integrate on a large scale in a clinical manner. Nevertheless, synergistic convergence of nanotechnology and additive manufacturing bodes well for a shift toward highly personalized, predictive, and effective medical treatments. This review discusses the revolutionary contribution of 3D printing nanotechnology in reframing the future of medicine with focus on the pressing need for an interdisciplinary team approach to realize its full capability. The epoch of tailoring personalized therapies to the molecular level is no longer an elusive vision but a swiftly realising expectation capable of radically redefining the delivery and outcomes of care at a worldwide scale.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"50 ","pages":"Article e00430"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144781419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting printability in suspended bioprinting using a rheology-informed hierarchical machine learning approach","authors":"Dageon Oh ,&nbsp;Dasong Kim ,&nbsp;Seung Yun Nam","doi":"10.1016/j.bprint.2025.e00427","DOIUrl":"10.1016/j.bprint.2025.e00427","url":null,"abstract":"<div><div>Suspended bioprinting has emerged as a promising method for overcoming the limitations of conventional extrusion-based bioprinting, enabling the creation of complex tissue constructs with improved resolution and shape fidelity. This technique utilizes a support bath to preserve the structural integrity of bioinks during deposition, allowing for the precise printing of low-viscosity materials. However, optimizing printability remains a significant challenge due to the absence of standardized methods and the complex interactions between bioink properties, support bath characteristics, and printing parameters. This study introduces a novel approach integrating suspended bioprinting with a rheology-informed hierarchical machine learning (RIHML) model to predict key printability factors such as axial resolution, horizontal resolution, and z-axis positional errors. A comprehensive dataset was generated by varying rheological properties and printing conditions to train and validate the RIHML model. The results show that the RIHML model outperforms conventional machine learning models, including support vector regression and concentration-dependent model, in predictive accuracy. This approach addresses critical challenges in suspended bioprinting, offering a scalable solution for improving printability, enhancing cost-effectiveness, reducing time consumption, and boosting the precision and reproducibility of tissue-engineered scaffolds.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"50 ","pages":"Article e00427"},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}