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Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg 石竹(Dianthus caryophyllus L.)基部腐病是由枯萎病(Fusarium verticillioides, Sacc.)引起的。Nirenberg
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.99009
Juan José Filgueira Duarte, Cindy Melissa Rincón-Sandoval, Carol Yineth Quinche, J. Soto, Ingrid Elizabeth Monroy
The carnation is one of the most important products for export in the floriculture industry of Colombia. Fusariosis (a disease resulting from presence of Fusarium) appears on the crops in two forms: vascular wilt and basal rot. The first is caused by Fusarium oxysporum f. sp. dianthi, which is a well-characterized disease. The second, caused by Fusarium verticillioides (previously Fusarium roseum), is a non-characterized disease and its development in the plant has not been described in detail. The symptoms of basal rot were differentiated from vascular wilt, through infection of plants in the greenhouse, with isolates of F. verticillioides and F. oxysporum obtained from lesions of symptomatic plants. The fungi morphological characterization allowed differentiation of the isolates of these two species and their growth habits on different media. The sequencing of 8 different genes with more than 13 amplicons in the 2 species showed genetic differences that grouped the isolates into different taxa. Multilocus sequence typing analysis using DNA sequences of 8 different genetic regions confirmed the presence of F. verticillioides. In this study, the role of F. verticillioides was demonstrated in the stems of carnation in commercial crops that presented pathogenic lesions. According to the results of the study, F. verticillioides is the etiological agent that produces the basal rotting in carnation plants, alone or in association with F. oxysporum.
康乃馨是哥伦比亚花卉产业中最重要的出口产品之一。镰刀菌病(一种由镰刀菌引起的疾病)在作物上以两种形式出现:维管枯萎病和基腐病。第一种是由尖孢镰刀菌引起的,这是一种特征明显的疾病。第二种是由黄萎病(Fusarium verticillioides)引起的,是一种非特征性疾病,其在植物中的发展尚未得到详细描述。通过对温室植物的侵染,从有症状植物的病损处分离出黄萎病菌和尖孢病菌,将基腐病的症状与维管束枯萎病区分开来。真菌的形态特征可以区分这两个物种的分离物及其在不同培养基上的生长习性。结果表明,两种菌株中8个扩增子大于13个的基因序列存在遗传差异,并将其归为不同的分类群。利用8个不同遗传区的DNA序列进行多位点序列分型分析,证实了黄萎病菌的存在。在这项研究中,证实了F. verticillioides在经济作物康乃馨茎中的作用。本研究结果表明,verticillioides是引起康乃馨基部腐烂的病原菌,可单独侵染或与尖孢镰刀菌联合侵染。
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引用次数: 0
Genetic variability of yam (Dioscorea trifida) genotypes in the Ucayali region, Peru 秘鲁乌卡亚利地区薯蓣(Dioscorea trifida)基因型的遗传变异
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.99877
L. L. Tuisima-Coral, Wilfredo Felipe Guillén Huachua
The aim of this research was to assess genetic variability of yam Dioscorea trifida genotypes using morphological descriptors for the germplasm collection conserved in the Agricultural Experiment Station in Ucayali, Peru. Thirty-eight morphological traits were evaluated for 30 D. trifida genotypes over ten years; from the data we estimated the Shannon-Weaver diversity index (H’) and the coefficient of variation and performed principal component analysis and cluster analysis. Qualitative traits with high phenotypic diversity index were: petiole anthocyanin (0.86), internal tuber color (0.86), petiole color (0.81) and stem color (0.80). The quantitative traits with the highest coefficient of variation were: tuber weight per plant (33.01) and tuber yield (32.99). Seventy-six percent of the morphological variability is explained by four principal components, the first component is constituted by the characters tuber width, tuber weight per plant, and tuber yield (29%). Five groups of genotypes were also identified with statistically significant differences, where group B stands out for its higher yield in fewer days to harvest. This research reveals wide morphological diversity in genotypes of D. trifida; these results can be used to strengthen the conservation, management, and genetic improvement initiatives of this important species in the Peruvian Amazon.
本研究的目的是利用形态描述子对秘鲁乌卡亚利农业试验站保存的山药薯蓣基因型的遗传变异进行评估。对30个三叶草基因型的38个形态性状进行了10年的评价;从数据中估计了Shannon-Weaver多样性指数(H’)和变异系数,并进行了主成分分析和聚类分析。表型多样性指数较高的质量性状为:叶柄花青素(0.86)、块茎内颜色(0.86)、叶柄颜色(0.81)和茎颜色(0.80)。变异系数最高的数量性状为:单株块茎重(33.01)和块茎产量(32.99)。其中,块茎宽度、单株块茎重和块茎产量是主要的性状组成部分(29%)。五组基因型也被确定为具有统计学显著差异,其中B组因其在更短的收获天数内获得更高的产量而突出。本研究揭示了三叶草基因型的广泛形态多样性;这些结果可用于加强秘鲁亚马逊地区这一重要物种的保护、管理和遗传改良计划。
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引用次数: 0
Design and development of a mixed alcoholic beverage kinetics using asaí (Euterpe precatoria) and copoazú (Theobroma grandiflorum) 利用asaí (Euterpe precatoria)和copoazú (Theobroma grandflorum)设计和开发混合酒精饮料动力学
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.98208
Willian Quintero Mendoza, Raquel Oriana Díaz-Salcedo, M. S. Hernández-Gómez
Copoazú (Theobroma grandiflorum), a fruit from the same genus as cacao, and asaí (Euterpe precatoria) a palm fruit, both of Amazonian origin, could promote local economic growth through fruit processing to increase the added value. This study aimed to identify the kinetics of alcoholic fruit beverages made from copoazú and asaí pulp or seeds, i.e., the fermentation kinetics in the case of copoazú drinks and the diffusion kinetics in the case of asaí drinks. Additionally, the feasibility of generating a milky mixture with the liquor obtained from the copoazú fruit processing was evaluated. Statistical analysis was performed by ANOVA tests and modeling of kinetics parameters with an evolutionary algorithm and optimization. Copoazú pulp was fermented with 15% Prestige Turbo Yeast®. Fermentation was separated into two stages: controlled fermentation during the first 5 d and a maturation process in the following 25 d. According to the modeling, the greatest efficiency was observed with 600 g L-1 pulp concentration and soluble solids adjusted at 35°Brix, with alcohol contents of up to 20% (w/v) after 30 d of processing and evidence that there may be inhibition of fermentation due to glycerol. The whole fruit and pulp of asaí were extracted with ethanol to obtain a liquor with the micronutrients and flavors of the fruit, and the anthocyanin content was used as a degradation process marker. Modelling showed that the optimum point that yielded maximum anthocyanin concentration was achieved at 60 d of maturation by extracting pulp in a 45% (w/v) ethanol solution resulting in a maximum anthocyanin content of 94.2 ± 15.3 mg of cyanidin-3-glucoside kg-1 of liquor. After that, a degradation process was observed as anthocyanin content diminished.
与可可同属的Copoazú(Theobroma grandiflorum)和棕榈树果实asaí(Euterpe pretoria)都原产于亚马逊地区,可以通过水果加工提高附加值来促进当地经济增长。本研究旨在确定由copoazú和asaí果肉或种子制成的酒精水果饮料的动力学,即copoazó饮料的发酵动力学和asaï饮料的扩散动力学。此外,还评估了用从copoazú水果加工中获得的液体产生乳白色混合物的可行性。通过方差分析测试进行统计分析,并使用进化算法和优化对动力学参数进行建模。Copoazú纸浆用15%Prestige Turbo酵母®发酵。发酵分为两个阶段:前5天的控制发酵和后25天的成熟过程。根据建模,在35°Brix下调节600 g L-1的果肉浓度和可溶性固体时,观察到最大的效率,加工30天后酒精含量高达20%(w/v),并且有证据表明可能由于甘油而抑制发酵。用乙醇提取asaí的整个果实和果肉,获得具有果实微量营养素和风味的酒,并以花青素含量作为降解过程的标志。模型显示,通过在45%(w/v)乙醇溶液中提取果肉,在成熟60天时达到产生最大花青素浓度的最佳点,从而产生94.2±15.3mg花青素-3-葡萄糖苷kg-1的最大花青素含量。之后,随着花青素含量的减少,观察到降解过程。
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引用次数: 0
Effect of biochar use as a substrate on granadilla (Passiflora ligularis Juss.) growth parameters 生物炭作为底物对西番莲生长参数的影响
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.98112
T. C. Puentes-Escobar, Adalberto Rodríguez-Carlosama, Camilo Andrés López
The impact of biochar on soils has been demonstrated, including its improvements of physical, chemical, and biological properties that promote agricultural production. This study aims to evaluate the effect of biochar on the growth of granadilla (Passiflora ligularis Juss.) seedlings. For this research, biochar was obtained from the pyrolysis of agricultural waste in a conical flame curtain reactor at temperatures between 400ºC and 500ºC for 90 min. The different biomasses used consisted of cholupa (Passiflora maliformis L.) fruit shells, residues of guamo (Inga spuria) wood, coffee (Coffea arabica L.) husks, and rice (Oryza sativa L.) husks. The biochar produced was mixed with Jiffy® brand peat in doses of 5%, 10%, and 20% (v/v) for each of the four types of biochar, with a control of 100% peat. For each treatment, 100 seedlings were planted, taking 12 random samples of each at 43, 57, and 71 d after sowing. Data were analyzed using the Kolmogorov-Smirnov and Levene test, followed by a factorial analysis of variance, evaluating variables such as dry weight, root length, leaf number, stem diameter, and chlorophyll index. The biochar obtained from the coffee husk promoted further growth, but its effectiveness decreased at a concentration of 20%.
生物炭对土壤的影响已经得到证明,包括其物理、化学和生物特性的改善,从而促进农业生产。本研究旨在评价生物炭对石榴幼苗生长的影响。在这项研究中,生物炭是在锥形火焰幕反应器中,在400ºC和500ºC之间的温度下热解农业废物90分钟获得的。所使用的不同生物物质包括芥子(Passiflora maliformis L.)果壳、番石榴(Inga spuria)木材残留物、咖啡(Coffea arabica L.)外壳和水稻(Oryza sativa L.)外壳。将生产的生物炭与Jiffy®牌泥炭混合,四种生物炭的剂量分别为5%、10%和20%(v/v),对照为100%泥炭。对于每种处理,种植100株幼苗,在播种后43、57和71天分别随机抽取12个样本。使用Kolmogorov-Smirnov和Levene检验对数据进行分析,然后进行方差因子分析,评估干重、根长、叶数、茎径和叶绿素指数等变量。从咖啡壳中获得的生物炭促进了进一步的生长,但在浓度为20%时其有效性降低。
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引用次数: 0
Dynamics of the weed community during pineapple growth in the Brazilian semi-arid region 巴西半干旱地区菠萝生长过程中杂草群落的动态
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.94079
João Rafael Prudêncio dos Santos, V. Maia, Bruno Soares da Silva, Pedro Mendes Demicheli, I. Aspiazú, G. Concenço
The pineapple belongs to the family Bromeliaceae and is a slow-growing succulent monocot with a reduced superficial root system. For this reason, the interference of weeds in competition with this crop can cause significant losses to the production. One of the bases to elaborate a control strategy is the knowledge of the diversity of weeds that occur in the cultivated areas. The objective of this study was to identify the weed community during pineapple growth in a semi-arid climate region of Brazil. Weeds were collected 60, 120, 180, 240, 300, and 360 days after planting (DAP) the pineapple. These collections were made in three different plots every two months until floral induction, composed of three pineapple cultivars. The weed community found in the irrigated pineapple field, in semi-arid climate conditions, was mostly composed by species belonging to the families Amaranthaceae, Asteraceae, Convolvulaceae, Fabaceae, Malvaceae, and Poaceae. The highest diversity of weed species was found at 60 DAP. The species Ipomoea acuminata was present throughout the development of the pineapple and showed the highest importance value index in most of the periods evaluated during the pineapple growth.
菠萝属于凤梨科,是一种生长缓慢的肉质单子叶植物,浅根系减少。因此,杂草干扰这种作物的竞争可能会对生产造成重大损失。制定控制策略的基础之一是了解种植区杂草的多样性。本研究的目的是确定巴西半干旱气候地区菠萝生长过程中的杂草群落。在种植(DAP)菠萝后60、120、180、240、300和360天收集杂草。这些集合每两个月在三个不同的地块上进行一次,直到由三个菠萝品种组成的花朵诱导。在半干旱气候条件下,灌溉菠萝地中发现的杂草群落主要由苋科、菊科、旋花科、蚕豆科、锦葵科和波科的物种组成。杂草种类多样性最高的是60DAP。Ipomoea acuminata物种在菠萝的整个发育过程中都存在,并且在菠萝生长过程中评估的大多数时期都表现出最高的重要性值指数。
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引用次数: 0
Postharvest application of acibenzolar-S-methyl and plant extracts affect physicochemical properties of blueberry (Vaccinium corymbosum L.) fruits 采后施用酸性苯并- s -甲基和植物提取物对蓝莓果实理化性质的影响
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.100044
Marilcen Jaime-Guerrero, J. Álvarez-Herrera, Hernán David Ruiz-Berrío
The demand for fruits with high anthocyanin content, such as blueberries, has increased in recent years due to their health benefits. However, few studies are known on the postharvest behavior of blueberry fruits subjected to the application of plant extracts and acibenzolar-S-methyl (ASM). The objective of this research was to investigate the effect of the application of ASM and vegetable extracts (mint and coriander) on the organoleptic characteristics of blueberry fruits stored at 16°C during postharvest through a completely  randomized design with five treatments. The contents of total anthocyanins in fruits (TA) did not differ between treatments, with values that ranged between 74.1 mg and 83.9 mg 100 g-1 of fresh weight. The TA tended to increase during storage and then to decrease during senescence. The firmness of blueberry fruits increased during storage, but hardness did not increase, since the fruits are softer and show wrinkling over time, indicating that the epidermis of the fruits has great elasticity. The fruits with the application of vegetable extracts showed the highest total soluble solids and total titratable acidity. The values of red/green and yellow/blue ratio increased, so the blueberry fruits slightly lost their blue hue during postharvest. Fruits with ASM application reached a postharvest life of 15 d after harvest (dah) while the other treatments only maintained quality for 13 dah.
近年来,由于对健康有益,人们对蓝莓等花青素含量高的水果的需求有所增加。然而,关于植物提取物和酸性苯并甲酯(ASM)对蓝莓果实采后行为的影响研究较少。本研究通过5种处理的完全随机设计,研究了ASM和植物提取物(薄荷和香菜)对采后16℃贮藏蓝莓果实感官特性的影响。果实中总花青素含量(TA)在不同处理间无显著差异,为74.1 ~ 83.9 mg / 100 g-1鲜重。TA在贮藏过程中呈上升趋势,在衰老过程中呈下降趋势。在贮藏过程中,蓝莓果实的硬度有所增加,但硬度没有增加,因为随着时间的推移,果实变软,并出现褶皱,表明果实表皮具有很大的弹性。施用植物提取物的果实可溶固形物总量和可滴定酸度最高。红/绿、黄/蓝比值增大,蓝莓果实采后蓝色调略有丧失。施用ASM的果实采后寿命可达15 d,而其他处理仅维持13 d的品质。
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引用次数: 3
Economic efficiency of biochar as an amendment for Acacia mangium Willd. plantations 生物炭改良剂马相思的经济效益。种植园
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.96330
Giovanni Reyes Moreno, Juan Carlos Barrientos Fuentes, Enrique Darghan Contreras
Biochar is a product of pyrolysis obtained from any type of biomass and can be used as a soil amendment or conditioner, improving the physical, chemical, and biological properties of the soil. Additionally, it can serve as an alternative to the application of synthetic fertilization in forest species such as Acacia mangium Willd. This research was oriented towards the determination of the economic efficiency of the use of biochar in A. mangium compared to the use of synthetic fertilizers. Production costs of wood and by-products, income and profits from forestry, economic efficiency of capital (cost-benefit ratio), labor (wood production per worker), and land (wood production ha-1) were considered. We found that the production of wood using biochar increased by 47% per unit area (ha), by 23% per unit of work (worker), and increased earnings by approximately one million Colombian pesos ha-1 compared to the use of only synthetic fertilizers.
生物炭是从任何类型的生物质中获得的热解产物,可以用作土壤改良剂或调理剂,改善土壤的物理、化学和生物特性。此外,它还可以作为替代人工施肥的一种方法应用于森林物种,如相思野生。本研究旨在确定与使用合成肥料相比,在mangium中使用生物炭的经济效率。考虑了木材及其副产品的生产成本、林业的收入和利润、资本(成本效益比)、劳动力(每个工人的木材产量)和土地(木材产量ha-1)的经济效率。我们发现,与仅使用合成肥料相比,使用生物炭生产木材的单位面积(公顷)增加了47%,单位劳动(工人)增加了23%,并且每公顷增加了约100万哥伦比亚比索的收入。
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引用次数: 0
Control of N-NH4+ and K+ leaching in potato using a carrageenan hydrogel 卡拉胶水凝胶控制马铃薯N-NH4+和K+浸出
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.98526
Nicolás Puentes Montealegre, Johanna Santamaría Vanegas, C. Ñústez-López, G. Rozo
Potato cultivation requires fertilizers to sustain crop yields, but a significant percentage of added nutrients is lost by leaching. The use of coating materials for fertilizers is currently being considered to reduce these losses. The objective of this study was to determine if a carrageenin based hydrogel (CBH), used to coat fertilizer, decreases NH4+ and K+ leaching from a potato crop without affecting growth, specific gravity, and tuber yield. The CBH was tested in a diploid potato crop, cultivar Criolla Colombia (Solanum tuberosum L., Phureja Group) using a randomized full block design including the treatments noncoated fertilizer (T1), CBH coated fertilizer (T2), and no fertilizer (T3). Mineral nutrients in soil leachates together with dry biomass, foliar area, chlorophyll, tuber specific gravity, and yield were quantified. The nutrient content in leachates from T2 were below those from T1. No significant differences between treatments were observed for growth factors, yield, and tuber specific gravity. This study confirms the controlling effect of the CBH, ensuring the retention of the nutrients added in the fertilizer and preventing them from easily leaching. Future field studies are worthwhile to establish the amount of fertilizer this coating could save.
马铃薯种植需要肥料来维持作物产量,但添加的养分有很大一部分因淋失而流失。目前正在考虑使用肥料的包衣材料来减少这些损失。本研究的目的是确定用于包衣肥料的卡拉胶基水凝胶(CBH)是否在不影响生长、比重和块茎产量的情况下减少了马铃薯作物的NH4+和K+淋出。以二倍体马铃薯品种哥伦比亚Criolla (Solanum tuberosum L., Phureja Group)为试验材料,采用随机全区设计,采用无包膜肥(T1)、包膜肥(T2)和无包膜肥(T3)处理。对土壤渗滤液中的矿质养分、干生物量、叶面积、叶绿素、块茎比重和产量进行了定量分析。T2渗滤液中营养物质含量低于T1渗滤液。在生长因子、产量和块茎比重方面,处理间无显著差异。本研究证实了CBH的控制作用,保证了肥料中添加的养分的保留,防止了养分的易淋失。未来的实地研究是有价值的,以确定该涂层可以节省多少肥料。
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引用次数: 0
Abundance of Beauveria spp. and Metarhizium spp. in maize and banana agroecosystems in central Cuba 古巴中部玉米和香蕉农业生态系统中白僵菌和绿僵菌的丰度
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.100886
Y. Ramos, A. Taibo, Andy Luis Alvarez Vega, Chabeli Abreu Lemes, R. Castañeda-Ruiz, O. Portal
Entomopathogenic fungi are an ecological alternative for the control of agricultural pests. These fungi live in organic matter in the soil and can cause natural epizootics in many arthropods associated with the rhizosphere. The aim of this study was to evaluate the abundance of Beauveria and Metarhizium spp. in maize and banana agroecosystems in central Cuba. Selective medium and insect baiting methods were used to isolate the entomopathogenic fungi from the soil. Metarhizium spp. were significantly more abundant than Beauveria spp. in both types of fields of agroecosystems. The abundance of Metarhizium spp. was higher in Sagua la Grande than in Santa Clara and Camajuaní municipalities. The insect bait method resulted as the most successful way to isolate entomopathogenic fungi from soil. These results show the composition of the entomopathogenic fungi in different agroecosystems, and they are an advance in the understanding of their ecology.
昆虫病原真菌是控制农业害虫的一种生态替代品。这些真菌生活在土壤中的有机物中,可以在许多与根际相关的节肢动物中引起自然流行病。本研究的目的是评估古巴中部玉米和香蕉农业生态系统中白僵菌和绿僵菌的丰度。采用选择性培养基和诱虫方法从土壤中分离出昆虫病原真菌。在这两种类型的农业生态系统中,绿僵菌的数量都明显高于白僵菌。绿僵菌的丰度在萨瓜拉格兰德市高于圣克拉拉市和卡马圭市。昆虫诱饵法是从土壤中分离昆虫病原真菌最成功的方法。这些结果显示了不同农业生态系统中昆虫病原真菌的组成,是对其生态学理解的进步。
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引用次数: 0
Plant growth and phosphorus uptake of coffee seedlings through mycorrhizal inoculation 菌根接种对咖啡幼苗生长和磷吸收的影响
Q4 Agricultural and Biological Sciences Pub Date : 2022-04-30 DOI: 10.15446/agron.colomb.v40n1.98599
H. González-Osorio, Carmenza Esther Góngora Botero, Sandra Patricia Jaramillo Padilla, W. Osorio
Soil phosphorus (P) availability is a limiting factor for coffee seedling growth. Usually, large amounts of P fertilizers are required, generating nutritional imbalance, increasing production costs, and raising environmental concerns in water pollution. The use of arbuscular mycorrhizal fungi (AMF) can enhance plant P uptake and growth and reduce the dose of P fertilizers. A greenhouse experiment was conducted in a substrate containing Paleudult soil and quartz sand, with low level of soluble P (1 mg kg-1), to establish the effect of AMF inoculation with Rhizoglomus fasciculatum on coffee (Coffea arabica L. cv. Colombia) seedlings growth and P uptake under three levels of P in soil solution (0.002, 0.02, and 0.2 mg L-1). AMF colonization was significantly reduced when contents of P in solution increased. Shoot dry weight and P foliar concentration were increased by the AMF inoculation when soil P in solution was 0.02 mg L-1; these effects were lower at 0.2 mg L-1 and null at 0.002 mg L-1 P. Results showed that AMF inoculation can play an important role in the growth of coffee seedlings as long as the content P in soil solution maintains intermediate level. At the lowest P level, the response of coffee seedlings to AMF inoculation was ineffective, while at the highest level, AMF application was unnecessary for coffee growth.
土壤磷的有效性是制约咖啡幼苗生长的一个因素。通常,需要大量的磷肥,这会造成营养失衡,增加生产成本,并引发水污染的环境问题。丛枝菌根真菌(AMF)的使用可以提高植物对磷的吸收和生长,并减少磷肥的用量。在含有低可溶性磷(1 mg kg-1)的Paleudult土壤和石英砂的基质中进行了温室试验,以确定在土壤溶液中三个磷水平(0.002、0.02和0.2 mg L-1)下,用束状根球菌接种AMF对咖啡(Coffea arabica L.cv.Colombia)幼苗生长和磷吸收的影响。当溶液中P含量增加时,AMF定植显著减少。当土壤磷浓度为0.02mg L-1时,接种AMF可提高茎干重和磷叶浓度;结果表明,只要土壤溶液中的磷含量保持在中等水平,AMF接种对咖啡幼苗的生长就起着重要作用。在最低的磷水平下,咖啡幼苗对AMF接种的反应是无效的,而在最高的磷水平上,AMF的应用对咖啡的生长是不必要的。
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引用次数: 1
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Agronomia Colombiana
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