Pub Date : 2024-09-04DOI: 10.1016/j.microc.2024.111564
Aynaz Niroumandpassand, Afshin Javadi, Mohammad Reza Afshar Mogaddam, Isa Fathollahi
The determination of antibiotic residues in animal–derived foods is an important issue in the field of food safety considering their residues side effects on consumers health. Therefore, recommendation an efficient analytical procedure for determination of these pollutants is of great importance. In the current work, a dispersive solid phase extraction in which bimetallic metal–organic framework is used as sorbent has been developed for extraction of some fluoroquinolone antibiotics from raw cow milk samples. The extracted analytes are determined using high performance liquid chromatography–fluorescence detector. The influence of exposing the milk samples to ultraviolet irradiations on the decontamination of antibiotics has also been investigated. Low limits of detection (5–11 ng L) and quantification (17–37 ng L), acceptable extraction recovery (63–76 %), and high repeatability (≤5.3 %) were accessed for analytes using the proposed analytical procedure under the optimum conditions. Various cow milk samples were analyzed with the proposed procedure under the optimum conditions and based on the outcomes, none of the tested samples were polluted with fluoroquinolone antibiotics. Also, the effect of samples matrix was evaluated by standard addition method and the mean relative recoveries were in the range of 86–102 %.
{"title":"Dispersive solid phase extraction of fluoroquinolone antibiotic residues in raw cow milk samples using bimetallic organic frameworks and investigating the effect of UV irradiation on antibiotics decontamination","authors":"Aynaz Niroumandpassand, Afshin Javadi, Mohammad Reza Afshar Mogaddam, Isa Fathollahi","doi":"10.1016/j.microc.2024.111564","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111564","url":null,"abstract":"The determination of antibiotic residues in animal–derived foods is an important issue in the field of food safety considering their residues side effects on consumers health. Therefore, recommendation an efficient analytical procedure for determination of these pollutants is of great importance. In the current work, a dispersive solid phase extraction in which bimetallic metal–organic framework is used as sorbent has been developed for extraction of some fluoroquinolone antibiotics from raw cow milk samples. The extracted analytes are determined using high performance liquid chromatography–fluorescence detector. The influence of exposing the milk samples to ultraviolet irradiations on the decontamination of antibiotics has also been investigated. Low limits of detection (5–11 ng L) and quantification (17–37 ng L), acceptable extraction recovery (63–76 %), and high repeatability (≤5.3 %) were accessed for analytes using the proposed analytical procedure under the optimum conditions. Various cow milk samples were analyzed with the proposed procedure under the optimum conditions and based on the outcomes, none of the tested samples were polluted with fluoroquinolone antibiotics. Also, the effect of samples matrix was evaluated by standard addition method and the mean relative recoveries were in the range of 86–102 %.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luteolin (3′,4′,5,7-tetrahydroxyflavone, LTL) is a kind of hydrophobic flavonoid, which has a number of pharmacological actions. It has a number of pharmacological actions, including anti-inflammatory, antioxidant, anticancer, and antihepatic fibrosis properties. High-dose LTL, on the other hand, can cause oxidative DNA damage and hydroxyl radical generation. It is critical to create easy, sensitive, and dependable LTL detection technologies and delivery methods for LTL quality control, clinical drug monitoring and enhancing drug bioavailability. In this work, a novel phenylboronic acid derived quantum dots (P-QDs) were synthesized for LTL sensitive detection and pH-responsive delivery. It showed remarkable selectivity and a brilliant fluorescence with a quantum yield of 43.62 %. In vitro experiments reveal negligible cytotoxicity. P-QDs and LTL coupling (P-QDs/LTL) showed powerful proliferation inhibition and migration restriction effects on HepG2 cells. In addition, P-QDs/LTL could time-dependently release it to restore fluorescence in the microacidic environment of HepG2 cells, thus demonstrating satisfactory fluorescence imaging capability. This strategy demonstrated a favourable linear relationship between LTL concentration and the fluorescence intensity of P-QDs in the range of 0.02–200 μM ( = 0.9952), with a detection limit of 1.16 nM (S/N=3). The recoveries in different real samples were in the range of 85.23 %-108.04 % with RSDs ≤6.1 %. The material is both a new attempt at LTL detection methodology and has significant promise for providing pH-responsive intracellular release of drug monitoring and cancer therapies from naked QDs without the requirement for sophisticated modified grafting.
{"title":"(B, N)-codoped carbon dots for sensitive luteolin detection and HepG2 cell imaging","authors":"Yongqiang Wang, Xingyu Hou, Yali Li, Xue Sun, Rui Hu, Yuguang Lv, Rui Jia, Lixin Ding","doi":"10.1016/j.microc.2024.111562","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111562","url":null,"abstract":"Luteolin (3′,4′,5,7-tetrahydroxyflavone, LTL) is a kind of hydrophobic flavonoid, which has a number of pharmacological actions. It has a number of pharmacological actions, including anti-inflammatory, antioxidant, anticancer, and antihepatic fibrosis properties. High-dose LTL, on the other hand, can cause oxidative DNA damage and hydroxyl radical generation. It is critical to create easy, sensitive, and dependable LTL detection technologies and delivery methods for LTL quality control, clinical drug monitoring and enhancing drug bioavailability. In this work, a novel phenylboronic acid derived quantum dots (P-QDs) were synthesized for LTL sensitive detection and pH-responsive delivery. It showed remarkable selectivity and a brilliant fluorescence with a quantum yield of 43.62 %. In vitro experiments reveal negligible cytotoxicity. P-QDs and LTL coupling (P-QDs/LTL) showed powerful proliferation inhibition and migration restriction effects on HepG2 cells. In addition, P-QDs/LTL could time-dependently release it to restore fluorescence in the microacidic environment of HepG2 cells, thus demonstrating satisfactory fluorescence imaging capability. This strategy demonstrated a favourable linear relationship between LTL concentration and the fluorescence intensity of P-QDs in the range of 0.02–200 μM ( = 0.9952), with a detection limit of 1.16 nM (S/N=3). The recoveries in different real samples were in the range of 85.23 %-108.04 % with RSDs ≤6.1 %. The material is both a new attempt at LTL detection methodology and has significant promise for providing pH-responsive intracellular release of drug monitoring and cancer therapies from naked QDs without the requirement for sophisticated modified grafting.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-04DOI: 10.1016/j.microc.2024.111567
Youwei Jiang, Chuanqin Zhou, Shuting Shi, Kun Wan, Aiting Chen, Nana Tang, Quanguo He
This research presents the development of a novel composite electrode material, CoNi(OH)/CeO/MoS-MWCNTs, synthesised via a two steps reflux process with ZIF-67 as precursor for the ultrasensitive detection of methyl parathion, a widely used organophosphorus pesticide. This electrode material demonstrates superior electrocatalytic activity and conductivity, enabling the rapid and accurate detection of methyl parathion residues in environmental and agricultural samples. The study extensively characterises the synthesised material using SEM, XRD, EDS, and EIS, confirming its unique nanostructure and elemental composition. The sensor exhibits a wide linear response range, high sensitivity, and a low detection limit of 0.1 nM, outperforming many existing methods. The practical application of the sensor is validated through objective sample analysis, showing high accuracy and reliability with recoveries between 98.4 % and 108 %. This work introduces a highly efficient sensor for methyl parathion detection and contributes to the broader application of nanocomposite materials in electrochemical sensing.
{"title":"CoNi(OH)x/CeO2/MoS2-MWCNTs composite electrode for ultrasensitive detection of methyl parathion in organophosphorus pesticides","authors":"Youwei Jiang, Chuanqin Zhou, Shuting Shi, Kun Wan, Aiting Chen, Nana Tang, Quanguo He","doi":"10.1016/j.microc.2024.111567","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111567","url":null,"abstract":"This research presents the development of a novel composite electrode material, CoNi(OH)/CeO/MoS-MWCNTs, synthesised via a two steps reflux process with ZIF-67 as precursor for the ultrasensitive detection of methyl parathion, a widely used organophosphorus pesticide. This electrode material demonstrates superior electrocatalytic activity and conductivity, enabling the rapid and accurate detection of methyl parathion residues in environmental and agricultural samples. The study extensively characterises the synthesised material using SEM, XRD, EDS, and EIS, confirming its unique nanostructure and elemental composition. The sensor exhibits a wide linear response range, high sensitivity, and a low detection limit of 0.1 nM, outperforming many existing methods. The practical application of the sensor is validated through objective sample analysis, showing high accuracy and reliability with recoveries between 98.4 % and 108 %. This work introduces a highly efficient sensor for methyl parathion detection and contributes to the broader application of nanocomposite materials in electrochemical sensing.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-04DOI: 10.1016/j.microc.2024.111568
Peng Bo Niu, Qian Cao, Long Huang, Chuan Hua Zhou, Jian Ling, Rong Hu, Tong Yang
Evaluating total antioxidant capacity (TAC) have been playing a vital role in health guidelines, the analysis of nutritional composition in foods, and the diagnosis and treatment of oxidative stress-induced disease. However, numerous antioxidants with similar physicochemical properties make them very difficult to be discriminated because of the similarly analytical signal output. In this work, a novel wavelength-dependent and three-channel photoelectrochemical (PEC) sensor array based on BiWO/Vo-TiO electrospun nanostructures was established for the identification of antioxidants in natural teas. The formation of heterojunction interfaces between BiWO nanosheet and Vo-TiO NFs, and the introduction of the oxygen vacancies in TiO NFs promoted the visible light absorption ability and electron transfer rate of BiWO/Vo-TiO NFs, causing the differences of photocurrent response to different antioxidants under different wavelengths. Furthermore, excited wavelengths, antioxidant types, and photocurrent were regarded as three-factors of fingerprint to accurately discriminate five antioxidants linear discriminant analysis (LDA). Finally, the PEC sensor array was able to estimate TAC in five actual tea samples (green, yellow, black, white, and dark teas). This development of LDA-assisted multiplex PEC fingerprinting sensor array showed a good promise for the identification and authentication of agricultural products, and the establishment of the database.
{"title":"Wavelength-dependent photoelectrochemical sensor array based on Bi2WO6/TiO2 electrospun nanoheterojunction for multiple antioxidants identification and total antioxidant capacity analysis in natural tea","authors":"Peng Bo Niu, Qian Cao, Long Huang, Chuan Hua Zhou, Jian Ling, Rong Hu, Tong Yang","doi":"10.1016/j.microc.2024.111568","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111568","url":null,"abstract":"Evaluating total antioxidant capacity (TAC) have been playing a vital role in health guidelines, the analysis of nutritional composition in foods, and the diagnosis and treatment of oxidative stress-induced disease. However, numerous antioxidants with similar physicochemical properties make them very difficult to be discriminated because of the similarly analytical signal output. In this work, a novel wavelength-dependent and three-channel photoelectrochemical (PEC) sensor array based on BiWO/Vo-TiO electrospun nanostructures was established for the identification of antioxidants in natural teas. The formation of heterojunction interfaces between BiWO nanosheet and Vo-TiO NFs, and the introduction of the oxygen vacancies in TiO NFs promoted the visible light absorption ability and electron transfer rate of BiWO/Vo-TiO NFs, causing the differences of photocurrent response to different antioxidants under different wavelengths. Furthermore, excited wavelengths, antioxidant types, and photocurrent were regarded as three-factors of fingerprint to accurately discriminate five antioxidants linear discriminant analysis (LDA). Finally, the PEC sensor array was able to estimate TAC in five actual tea samples (green, yellow, black, white, and dark teas). This development of LDA-assisted multiplex PEC fingerprinting sensor array showed a good promise for the identification and authentication of agricultural products, and the establishment of the database.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-04DOI: 10.1016/j.microc.2024.111565
Mohammad Rouhi Maleki, Mohammadhosein Movassaghghazani, Mohammad Reza Afshar Mogaddam
A deep eutectic solvent-based ultrasonic assisted liquid–liquid microextraction method was developed to extract organochlorine pesticides from cheese samples before their quantification with gas chromatography–mass spectrometry. A mixture of deionized water, potassium hydroxide solution, and thymol: decanoic acid deep eutectic solvent was used in the method development. Under the optimum method conditions, acceptable precision was achieved, with intra– and inter-day precisions falling within the ranges of 2.9–4.3 % and 3.5–4.7 %, respectively). A good coefficient of determination (r ≥ 0.993) was obtained over a wide concentration range (107–250,000 ng kg), with low limits of detection (32–58 ng kg) and quantification (107–193 ng kg). Additionally, acceptable extraction recoveries (57–79 %) were achieved. Method validation was conducted and the method was successfully applied to several cheese samples. Based on the results, all of the investigated samples were found to be free of investigated organochlorine residues.
开发了一种基于深共晶溶剂的超声辅助液液微萃取方法，用于萃取奶酪样品中的有机氯农药，然后用气相色谱-质谱法进行定量。该方法采用去离子水、氢氧化钾溶液和百里酚：癸酸深度共晶溶剂的混合物。在最佳方法条件下，达到了可接受的精密度，日内和日间精密度分别在 2.9-4.3 % 和 3.5-4.7 % 范围内。）在较宽的浓度范围（107-250,000 ng kg）内，测定系数良好（r ≥ 0.993），检出限（32-58 ng kg）和定量限（107-193 ng kg）较低。此外，萃取回收率（57-79%）也达到了可接受的水平。对该方法进行了验证，并成功应用于几种奶酪样品。结果表明，所有调查样品均不含所调查的有机氯残留物。
{"title":"Development of deep eutectic solvent-based ultrasonic assisted liquid–liquid microextraction coupled with GC–MS; application in analysis of organochlorine pesticides from cheese samples","authors":"Mohammad Rouhi Maleki, Mohammadhosein Movassaghghazani, Mohammad Reza Afshar Mogaddam","doi":"10.1016/j.microc.2024.111565","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111565","url":null,"abstract":"A deep eutectic solvent-based ultrasonic assisted liquid–liquid microextraction method was developed to extract organochlorine pesticides from cheese samples before their quantification with gas chromatography–mass spectrometry. A mixture of deionized water, potassium hydroxide solution, and thymol: decanoic acid deep eutectic solvent was used in the method development. Under the optimum method conditions, acceptable precision was achieved, with intra– and inter-day precisions falling within the ranges of 2.9–4.3 % and 3.5–4.7 %, respectively). A good coefficient of determination (r ≥ 0.993) was obtained over a wide concentration range (107–250,000 ng kg), with low limits of detection (32–58 ng kg) and quantification (107–193 ng kg). Additionally, acceptable extraction recoveries (57–79 %) were achieved. Method validation was conducted and the method was successfully applied to several cheese samples. Based on the results, all of the investigated samples were found to be free of investigated organochlorine residues.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-03DOI: 10.1016/j.microc.2024.111559
Saithip Pakapongpan, Yingyot Poo-arporn, Sunatcha Ninket, Rungtiva P. Poo-arporn
This work presented a molecularly imprinted polymer (MIP) electrochemical sensor for determination of amyloid-β42 (Aβ42) as an Alzheimer’s disease (AD) biomarker. A nitrogen-doped carbon-dot-graphene (NCD-G) nanohybrid was synthesized through a simple ultrasonic method. Polypyrrole (PPY) was used as an imprinted polymer with an Aβ42 template for the electropolymerization process. The NCD-G and MIP were modified on a screen-printed carbon electrode (SPCE) without binding agent. The morphology and electrochemical properties of MIP/NCD-G modified SPCE were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis spectroscopy, X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV) and square wave voltammetry (SWV). The developed sensor exhibited good linear response in a range from 5 to 70 pg/mL with a limit of detection (LOD) of 1 pg/mL under optimal conditions. This sensor shows excellent performance due to the enhancement of effective surface area, electrical conductivity, and electrocatalytic activity. This MIP sensor takes advantage of molecularly imprinted technique combined with nanohybrid to achieve high selectivity and sensitivity. Moreover, it possessed good reproducibility and miniaturization that could be applied as a platform for portable devices in healthcare in the future. This work provides the combination of a zero-dimensional and two-dimensional materials, nitrogen doped carbon dots-graphene nanohybrid (NCD-G) by self-assembly through a simple ultrasonication method and applied as a molecularly imprinted polymer (MIP) sensor for determination of amyloid-β42 protein. We propose a minimum number of preparation steps which NCD-G could enhance the MIP sensor performance, owing to the synergistic effects with high selectivity and sensitivity. The hybrid formation of the carbon dots with graphene using their π-π interactions in carbon nanostructures. NCD-G nanohybrid and MIP modified on screen printed carbon electrode (SPCE) for the determination of amyloid-β42 have not been previously investigated. Moreover, this disposable strip is a single-use tool that might be essential for on-site devices.
{"title":"A disposable electrochemical sensor for amyloid-β42 protein based on molecular imprinted polymers with nitrogen doped carbon dots-graphene nanohybrid","authors":"Saithip Pakapongpan, Yingyot Poo-arporn, Sunatcha Ninket, Rungtiva P. Poo-arporn","doi":"10.1016/j.microc.2024.111559","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111559","url":null,"abstract":"This work presented a molecularly imprinted polymer (MIP) electrochemical sensor for determination of amyloid-β42 (Aβ42) as an Alzheimer’s disease (AD) biomarker. A nitrogen-doped carbon-dot-graphene (NCD-G) nanohybrid was synthesized through a simple ultrasonic method. Polypyrrole (PPY) was used as an imprinted polymer with an Aβ42 template for the electropolymerization process. The NCD-G and MIP were modified on a screen-printed carbon electrode (SPCE) without binding agent. The morphology and electrochemical properties of MIP/NCD-G modified SPCE were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis spectroscopy, X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV) and square wave voltammetry (SWV). The developed sensor exhibited good linear response in a range from 5 to 70 pg/mL with a limit of detection (LOD) of 1 pg/mL under optimal conditions. This sensor shows excellent performance due to the enhancement of effective surface area, electrical conductivity, and electrocatalytic activity. This MIP sensor takes advantage of molecularly imprinted technique combined with nanohybrid to achieve high selectivity and sensitivity. Moreover, it possessed good reproducibility and miniaturization that could be applied as a platform for portable devices in healthcare in the future. This work provides the combination of a zero-dimensional and two-dimensional materials, nitrogen doped carbon dots-graphene nanohybrid (NCD-G) by self-assembly through a simple ultrasonication method and applied as a molecularly imprinted polymer (MIP) sensor for determination of amyloid-β42 protein. We propose a minimum number of preparation steps which NCD-G could enhance the MIP sensor performance, owing to the synergistic effects with high selectivity and sensitivity. The hybrid formation of the carbon dots with graphene using their π-π interactions in carbon nanostructures. NCD-G nanohybrid and MIP modified on screen printed carbon electrode (SPCE) for the determination of amyloid-β42 have not been previously investigated. Moreover, this disposable strip is a single-use tool that might be essential for on-site devices.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-03DOI: 10.1016/j.microc.2024.111560
Qijun He, Bin Du, Wei Hu, Lingxiao Liu, Bing Liu, Xihui Mu, Zhaoyang Tong, Rabigul Tursun, Jianjie Xu
Bacteria-induced food pollution poses a serious threat to human health. To detect early and sensitive traces of bacteria in food, a poly (ethyleneimine) (PEI)-modified magnetic organic framework material (FeO@UiO-66-NH@PEI) was prepared for broad-spectrum enrichment and dual-mode detection of typical gram-positive bacteria (Bacillus subtilis, B. subtilis) and gram-negative bacteria (Escherichia coli, E. coli) using Schiff’s base reaction. Due to the positive charge caused by the abundance of amino groups, FeO@UiO-66-NH@PEI exhibited rapid enrichment of E. coli and B. subtilis within 12 min through the electrostatic interaction with bacteria. The biosensor based on FeO@UiO-66-NH@PEI achieved sensitive detection of E. coli and B. subtilis using the adenosine triphosphate (ATP) bioluminescence technology within the detection range of 10-10, and the detection limits were 44 and 57 CFU/mL, respectively under optimal experimental conditions. Moreover, a fluorescence platform was constructed by FeO@UiO-66-NH@PEI to achieve more diversified detection and reliable detection results for E. coli and B. subtilis. Within the detection range of 10-10, FeO@UiO-66-NH@PEI exhibited satisfactory linearity with detection limits of 6 CFU/mL and 14 CFU/mL. This biosensor using ATP bioluminescence and fluorescence technology showed remarkable recoveries (85.3–108.5 %) for E. coli and B. subtilis in biscuit matrices, indicating a bright prospect of the dual-mode platform for bacteria detection in food.
{"title":"An ATP-fluorescence dual-mode biosensing platform for rapid broad-spectrum enrichment and detection of bacteria based on PEI-functionalized magnetic UiO-66-NH2","authors":"Qijun He, Bin Du, Wei Hu, Lingxiao Liu, Bing Liu, Xihui Mu, Zhaoyang Tong, Rabigul Tursun, Jianjie Xu","doi":"10.1016/j.microc.2024.111560","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111560","url":null,"abstract":"Bacteria-induced food pollution poses a serious threat to human health. To detect early and sensitive traces of bacteria in food, a poly (ethyleneimine) (PEI)-modified magnetic organic framework material (FeO@UiO-66-NH@PEI) was prepared for broad-spectrum enrichment and dual-mode detection of typical gram-positive bacteria (Bacillus subtilis, B. subtilis) and gram-negative bacteria (Escherichia coli, E. coli) using Schiff’s base reaction. Due to the positive charge caused by the abundance of amino groups, FeO@UiO-66-NH@PEI exhibited rapid enrichment of E. coli and B. subtilis within 12 min through the electrostatic interaction with bacteria. The biosensor based on FeO@UiO-66-NH@PEI achieved sensitive detection of E. coli and B. subtilis using the adenosine triphosphate (ATP) bioluminescence technology within the detection range of 10-10, and the detection limits were 44 and 57 CFU/mL, respectively under optimal experimental conditions. Moreover, a fluorescence platform was constructed by FeO@UiO-66-NH@PEI to achieve more diversified detection and reliable detection results for E. coli and B. subtilis. Within the detection range of 10-10, FeO@UiO-66-NH@PEI exhibited satisfactory linearity with detection limits of 6 CFU/mL and 14 CFU/mL. This biosensor using ATP bioluminescence and fluorescence technology showed remarkable recoveries (85.3–108.5 %) for E. coli and B. subtilis in biscuit matrices, indicating a bright prospect of the dual-mode platform for bacteria detection in food.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-03DOI: 10.1016/j.microc.2024.111545
Mamajan Ovezova, Fatma Yılmaz, Ilgım Göktürk, Adil Denizli
C-reactive protein (CRP) level provides important information about the health status of the individual in predicting many diseases such as cardiovascular, chronic inflammatory, and neurodegenerative diseases. Therefore, determining CRP levels is important for correct health intervention and treatment follow-up. For the selective detection of CRP, we developed a (GO/Au-MIP) SPR sensor containing CRP-imprinted polymer modified with graphene oxide and gold nanoparticles. To prove the enhanced sensitivity of the sensor resulting from the presence of graphene oxide (GO) and gold nanoparticles (AuNPs), a CRP-imprinted (MIP) SPR sensor was prepared using the same method, excluding the incorporation of GO and AuNPs. In addition, a non-imprinted GO/Au-NIP SPR sensor was also prepared to evaluate the imprinting efficiency. In detecting CRP in PBS buffer, the GO/Au-MIP SPR sensor exhibited linearity in the concentration ranges of 0.1–2 ppm (R = 0.9721) and 5–100 ppm (R = 0.9740). The detection limit of the prepared sensor was calculated as 0.0082 ppm. In the study, the imprinting process efficiency was also evaluated by calculating the imprinting factor value (I.F=13.84). In the selectivity studies, the GO/Au-MIP SPR sensor was determined to be 9.23 times more selective against CRP protein than bovine serum albumin and 29.53 times more selective than hemoglobin. When the repeatability of the GO/Au-MIP SPR sensor was examined, it was determined that the GO/Au-MIP SPR sensor was able to detect CRP without any deterioration in performance in five consecutive reuses (RSD<1.5). Finally, CRP detection studies from the serum and urine solutions, which were selected as real samples, were carried out by the GO/Au-MIP SPR sensor to evaluate the matrix effect. CRP spiked serum sample was analyzed by the other standard method to validate the analytical results using CRP analyser.
{"title":"Plasmonic sensing platform for C-reactive protein recognition via synthetic receptors decorated on graphene oxide and gold nanoparticles","authors":"Mamajan Ovezova, Fatma Yılmaz, Ilgım Göktürk, Adil Denizli","doi":"10.1016/j.microc.2024.111545","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111545","url":null,"abstract":"C-reactive protein (CRP) level provides important information about the health status of the individual in predicting many diseases such as cardiovascular, chronic inflammatory, and neurodegenerative diseases. Therefore, determining CRP levels is important for correct health intervention and treatment follow-up. For the selective detection of CRP, we developed a (GO/Au-MIP) SPR sensor containing CRP-imprinted polymer modified with graphene oxide and gold nanoparticles. To prove the enhanced sensitivity of the sensor resulting from the presence of graphene oxide (GO) and gold nanoparticles (AuNPs), a CRP-imprinted (MIP) SPR sensor was prepared using the same method, excluding the incorporation of GO and AuNPs. In addition, a non-imprinted GO/Au-NIP SPR sensor was also prepared to evaluate the imprinting efficiency. In detecting CRP in PBS buffer, the GO/Au-MIP SPR sensor exhibited linearity in the concentration ranges of 0.1–2 ppm (R = 0.9721) and 5–100 ppm (R = 0.9740). The detection limit of the prepared sensor was calculated as 0.0082 ppm. In the study, the imprinting process efficiency was also evaluated by calculating the imprinting factor value (I.F=13.84). In the selectivity studies, the GO/Au-MIP SPR sensor was determined to be 9.23 times more selective against CRP protein than bovine serum albumin and 29.53 times more selective than hemoglobin. When the repeatability of the GO/Au-MIP SPR sensor was examined, it was determined that the GO/Au-MIP SPR sensor was able to detect CRP without any deterioration in performance in five consecutive reuses (RSD<1.5). Finally, CRP detection studies from the serum and urine solutions, which were selected as real samples, were carried out by the GO/Au-MIP SPR sensor to evaluate the matrix effect. CRP spiked serum sample was analyzed by the other standard method to validate the analytical results using CRP analyser.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The rapid detection of key indicators during the fermentation is crucial for improving product quality. In light of this, a strategy was developed for the rapid detection of -Theanine during kombucha fermentation using surface-enhanced Raman Scattering (SERS) coupled with machine/deep learning methodologies. After analyzing the attribution of -Theanine SERS characteristic peaks, effective variable features, converted features, and depth features were extracted, and calibration models were constructed employing Partial Least Squares (PLS), Support Vector Machine (SVM), and a one-dimensional Convolutional Neural Network (1DCNN). Based on the first 10 principal components derived via principal component analysis (PCA), the established model, PCA10-1DCNN, displayed superior performance with a determination coefficient (R) of 0.9750. PCA10-1DCNN achieved commendable verification efficacy, with a maximum error of 4.4460 µg/mL, indicating the efficacy of this strategy for the -Theanine rapid detection. This study offers a viable strategy for the -Theanine rapid detection in the production of tea-based beverages.
{"title":"A rapid method for detecting l-Theanine during kombucha fermentation using SERS combined with machine/deep learning","authors":"Songguang Zhao, Tianhui Jiao, Selorm Yao-Say Solomon Adade, Zhen Wang, Xiaoxiao Wu, Qin Ouyang, Quansheng Chen","doi":"10.1016/j.microc.2024.111557","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111557","url":null,"abstract":"The rapid detection of key indicators during the fermentation is crucial for improving product quality. In light of this, a strategy was developed for the rapid detection of -Theanine during kombucha fermentation using surface-enhanced Raman Scattering (SERS) coupled with machine/deep learning methodologies. After analyzing the attribution of -Theanine SERS characteristic peaks, effective variable features, converted features, and depth features were extracted, and calibration models were constructed employing Partial Least Squares (PLS), Support Vector Machine (SVM), and a one-dimensional Convolutional Neural Network (1DCNN). Based on the first 10 principal components derived via principal component analysis (PCA), the established model, PCA10-1DCNN, displayed superior performance with a determination coefficient (R) of 0.9750. PCA10-1DCNN achieved commendable verification efficacy, with a maximum error of 4.4460 µg/mL, indicating the efficacy of this strategy for the -Theanine rapid detection. This study offers a viable strategy for the -Theanine rapid detection in the production of tea-based beverages.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-02DOI: 10.1016/j.microc.2024.111544
Audrey Grandjean, Anaïs Becker, Coralie Kustner, Michel Wolf, Christophe Sutter, Romain Severac, Jean-Philippe Amiet, Damien Bazin, Stéphane Le Calvé
Formaldehyde is a major indoor air pollutant. Accurate analysis of airborne formaldehyde is essential for effective monitoring and risk assessment. For many formaldehyde analysis techniques, the accuracy of the results depends on the calibration system. Existing formaldehyde calibration methods often face limitations, such as instability over time or limited concentration range. They also lack practicality for on-field calibration either because of bulkiness or because of high gas consumption. To address these challenges, a device using a paraformaldehyde permeation tube within a custom-built, temperature-controlled system was designed and evaluated.
{"title":"Portable temperature-controlled permeation device for the generation of formaldehyde gas standard","authors":"Audrey Grandjean, Anaïs Becker, Coralie Kustner, Michel Wolf, Christophe Sutter, Romain Severac, Jean-Philippe Amiet, Damien Bazin, Stéphane Le Calvé","doi":"10.1016/j.microc.2024.111544","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111544","url":null,"abstract":"Formaldehyde is a major indoor air pollutant. Accurate analysis of airborne formaldehyde is essential for effective monitoring and risk assessment. For many formaldehyde analysis techniques, the accuracy of the results depends on the calibration system. Existing formaldehyde calibration methods often face limitations, such as instability over time or limited concentration range. They also lack practicality for on-field calibration either because of bulkiness or because of high gas consumption. To address these challenges, a device using a paraformaldehyde permeation tube within a custom-built, temperature-controlled system was designed and evaluated.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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