Microchemical Journal最新文献_第6页

Application of 3D structure of carbon nanotube-interwoven metal–organic frameworks in solid-phase microextraction of trace polychlorinated biphenyls from tea beverages 碳纳米管交织金属有机框架三维结构在茶饮料中痕量多氯联苯固相微萃取中的应用

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-23 DOI: 10.1016/j.microc.2024.111752

Xin-Li Song , Fei-Yan He , Yu-Qing Liu , Ke-Chao Liao , Dong-Dong Wang , Xue-Shan Wang , Hui Lv , Ting-Ting Sun , Jing-Long Wang , Li-Hua Zhang , Han Zhang , Yue Chen

A three-dimensional (3D) structure of multiwalled carbon nanotubes (MWCNTs) interwoven metal–organic frameworks (MOFs) was synthesized. The MWCNTs was utilized to bridge the neighboring MOF nanocrystals. The hybrid material MOFs/MWCNTs was used for SPME coating to extract six PCB_S in tea beverages samples by gas chromatography coupled to tandem mass spectrometry. MOFs/MWCNTs coating materials were characterized by thermogravimetric analysis (TGA), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and N₂ adsorption–desorption analysis. The response surface method was utilized to systematically optimize the main extraction factors. Under the optimized conditions, the developed method demonstrated wide linear ranges (0.01–1000 ng·L⁻¹), low limits of detection (0.003–0.009 ng·L⁻¹) and high enrichment factors (701–2361) for extracting and determining six PCB_S. The six PCB_S concentration in environmental tea beverages samples was detected with this method. These results display that MOFs/MWCNTs hybrid materials are potential sorbents in the SPME of PCB_S in environmental tea beverages samples.

多壁碳纳米管（MWCNTs）交织金属有机框架（MOFs）的三维（3D）结构被合成出来。多壁碳纳米管被用来桥接相邻的 MOF 纳米晶体。将 MOFs/MWCNTs 混合材料用于 SPME 涂层，通过气相色谱-串联质谱法提取茶饮料样品中的六种 PCBS。通过热重分析（TGA）、扫描电子显微镜（SEM）、傅立叶变换红外光谱（FTIR）、X射线衍射（XRD）和N2吸附-解吸分析对MOFs/MWCNTs涂层材料进行了表征。利用响应面法对主要萃取因子进行了系统优化。结果表明,在优化条件下,所建立的方法萃取测定6种PCBS的线性范围宽(0.01～1000 ng-L-1),检出限低(0.003～0.009 ng-L-1),富集因子高(701～2361)。该方法检测出了环境茶饮料样品中六种 PCBS 的浓度。这些结果表明，MOFs/MWCNTs 杂化材料是环境茶饮料样品中 PCBS SPME 的潜在吸附剂。

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引用次数: 0

New advances in the enhancement of direct mercury speciation in solid matter on the example of HgCl, CH3HgCl, HgS and HgSO4 using programmable thermal release in combination with electrothermal atomic absorption spectrometry 以 HgCl、CH3HgCl、HgS 和 HgSO4 为例，利用可编程热释放技术结合电热原子吸收光谱法，在提高固体物质中的直接汞标示方面取得新进展

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-23 DOI: 10.1016/j.microc.2024.111755

Olga V. Shuvaeva , Ivan A. Bekesha , Dmitrii Yu. Troitskii

Mercury is considering as hazardous pollutant presented as a number of various species characterized by different migration routes, bioavailability and toxicity. In this regard, the characterization of natural and a fortiori anthropogenic environment by the concentration of various chemical forms of mercury is relevant to assess the degree of its impact on living organisms. From this point of view, solid samples are the most problematic ones due to the need for preliminary extraction of analytes into solution. The promising approach for this purpose is analysis based on a combination of thermal release as separation way with sequential electrothermal atomic absorption spectrometric detection (TR-ETA-AAS). The present work is based on research of the thermal behavior of individual mercury species in argon atmosphere when the evaporation and atomization zones are separated and a programmable heating algorithm is implemented. To unify an analytical procedure the dilution of reference and analyzed samples with aluminum oxide was applied. The developed method allows determination of the species most often found in natural and contaminated environments: mercury (II) chloride, methylmercury chloride, mercury sulfide and sulphate at the level of 0.003–0.030 µg with a reproducibility of 10–25 %,. The trueness of the proposed approach has been confirmed using certified reference material ERM CE464 and spiking method.

汞被认为是一种危险的污染物，它有许多不同的种类，具有不同的迁移路径、生物利用率和毒性。因此，通过各种化学形式的汞浓度来描述自然环境和人为环境的特征，对于评估汞对生物体的影响程度非常重要。从这个角度来看，固体样本最容易出现问题，因为需要将分析物初步提取到溶液中。为此，最有前途的方法是将热释放作为分离方式与顺序电热原子吸收光谱检测（TR-ETA-AAS）相结合进行分析。当蒸发区和雾化区分离并采用可编程加热算法时，本研究工作基于对氩气环境中单个汞物种热行为的研究。为了统一分析程序，采用了氧化铝稀释参考样品和分析样品的方法。所开发的方法可以在 0.003-0.030 µg 的水平上测定自然环境和污染环境中最常见的物种：氯化汞 (II)、氯化甲基汞、硫化汞和硫酸盐，重现性为 10-25%。使用认证参考材料 ERM CE464 和加标法确认了拟议方法的准确性。

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引用次数: 0

UHPLC method for simultaneous assessment of pharmacokinetic parameters of co-administered voriconazole and omeprazole using rat plasma 利用超高效液相色谱法同时评估大鼠血浆中同时服用伏立康唑和奥美拉唑的药代动力学参数

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111729

Qing Qian , Lanlan Xu , Xianhua Che , Fang Liu , Xuezheng Li

Voriconazole (VRC) is a first-line therapeutic agent for the treatment of invasive fungal infections, whereas omeprazole (OMZ) is a commonly used acid suppressant; however, the two drugs are often used in combination in clinical practice. The aim of this research was to investigate how the co-administration of OMZ and VRC affects the pharmacokinetic characteristics of VRC in rats. A new ultra high-performance liquid chromatography (UHPLC) analytical method was developed and validated for simultaneous analysis of co-administered drugs using VRC and OMZ. A Shim-pack GIST-HP C18 column with 0.1 M triethylamine:acetonitrile (70:30, v/v) as the mobile phase (flow rate: 0.3 mL/min) was used, and UV detection was performed at 240 nm. Liquid–liquid extraction of plasma samples was carried out using dichloromethane. The bioanalytical method was linear over a range of VRC concentrations (100–2000 ng/mL) and OMZ (50–10,000 ng/mL) concentrations, and exhibited both accuracy and precision within the acceptable respective ranges. The average extraction recoveries for VRC and OMZ in plasma were 94.88 % and 82.76 %, respectively. Additionally, the method was successfully applied in vivo to estimate the pharmacokinetic features of VRC in the plasma of rats receiving gavage with low and high doses of OMZ. In conclusion, using a new UHPLC method, we determined that co-administration of OMZ substantially decreased the bioavailability of VRC in rats. Potentially significant drug interactions should be considered in patients receiving the combination of OMZ and VRC.

伏立康唑（Voriconazole，VRC）是治疗侵袭性真菌感染的一线治疗药物，而奥美拉唑（Omeprazole，OMZ）是一种常用的抑酸剂；然而，在临床实践中，这两种药物经常联合使用。本研究旨在探讨大鼠同时服用 OMZ 和 VRC 如何影响 VRC 的药代动力学特征。研究人员开发了一种新的超高效液相色谱（UHPLC）分析方法，并对该方法进行了验证。采用Shim-pack GIST-HP C18色谱柱，以0.1 M三乙胺：乙腈（70:30, v/v）为流动相（流速：0.3 mL/min），紫外检测波长为240 nm。血浆样品采用二氯甲烷进行液液萃取。该生物分析方法在 VRC 浓度（100-2000 纳克/毫升）和 OMZ 浓度（50-10000 纳克/毫升）范围内线性关系良好，准确度和精密度均在可接受的范围内。血浆中VRC和OMZ的平均提取回收率分别为94.88%和82.76%。此外，该方法还成功地用于体内大鼠灌胃低剂量和高剂量OMZ后血浆中VRC药代动力学特征的估计。总之，通过使用一种新的超高效液相色谱法，我们确定了同时给药 OMZ 会大大降低大鼠体内 VRC 的生物利用度。接受 OMZ 和 VRC 联合用药的患者应考虑到潜在的重大药物相互作用。

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引用次数: 0

A different application of the Aliquat® 336 loaded poly(vinylidene fluoride-co-hexafluoropropylene) based electrospun nanofibers disks for the preconcentration followed by flame atomic absorption spectrometry detection of low-level Bi(III) Aliquat® 336负载聚（偏氟乙烯-六氟丙烯）电纺纳米纤维盘在火焰原子吸收光谱法检测低浓度Bi(III)时的不同应用

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111703

Jamal-Addin Rajabi-Orhani , Leila Dolatyari , Mohammad Reza Yaftian

It is the first report on the potential of electrospun nanofiber disks inserted in a filtration apparatus as a preconcentration strategy for the analysis of low-level metals. The nanofibers included 60 wt% of poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) and 40 wt% of ionic liquid Aliquat® 336. The electrospinning process was done by applying a voltage of 16 kV, an injection rate of 1 mL h⁻¹, and 10 cm distance of the needle tip location from the drum collector. A circular piece of the prepared nanofiber disks was placed in a filtration apparatus, and 200 mL solution containing 1 mg of Bi(III) adjusted to 0.5 mol/L of hydrochloric acid was transmitted from inside the nanofiber disk by controlling the flowrate (3.3 mL s⁻¹). The adsorbed Bi(III) was eluted by 10 mL of 0.2 mol/L solution of ethylendiaminetetraacetic acid and ammonium hydroxide mixture, and then was analyzed by flame atomic absorption spectrometry. The introduced technique (nanofiber disks-based solid phase extraction) can be used in the bismuth concentration range of 4–2000 µg/L, with a limit of detection 1.2 µg/L. The determined inter- and intra-day relative standard deviations (3 days and n = 7) for a solution of 100 µg/L of bismuth were 1.1 % and 2.4 %, respectively. The method offers a preconcentration factor of 200-fold. The fabricated nanofiber disks were used at least in 7 consecutive preconcentration experiments. The proposed method was selective towards Bi(III) and can be used satisfactory for its determination in real samples.

这是首次报道在过滤装置中插入电纺纳米纤维盘作为低浓度金属分析预浓缩策略的潜力。纳米纤维包括 60% 的聚（偏氟乙烯-六氟丙烯）（PVDF-HFP）和 40% 的离子液体 Aliquat® 336。电纺过程的电压为 16 kV，注射速率为 1 mL h-1，针尖位置与鼓式收集器的距离为 10 cm。将制备好的纳米纤维圆片置于过滤装置中，通过控制流速（3.3 mL s-1），从纳米纤维圆片内部导入 200 mL 含有 1 mg Bi（III）的盐酸溶液（浓度为 0.5 mol/L）。用 10 mL 0.2 mol/L 的乙二胺四乙酸和氢氧化铵混合溶液洗脱吸附的 Bi(III)，然后用火焰原子吸收光谱法进行分析。所引入的技术（基于纳米纤维盘的固相萃取）可用于 4-2000 µg/L 的铋浓度范围，检测限为 1.2 µg/L。对于 100 µg/L 的铋溶液，测定的日间和日内相对标准偏差（3 天，n = 7）分别为 1.1 % 和 2.4 %。该方法的预浓缩倍数为 200 倍。制备的纳米纤维盘至少连续使用了 7 次预浓缩实验。所提出的方法对 Bi(III)具有良好的选择性，可用于实际样品中 Bi(III)的测定。

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引用次数: 0

A comprehensive review on quantification of various biomarkers for the detection of oral carcinoma via electrochemical biosensors 通过电化学生物传感器定量检测口腔癌的各种生物标志物综述

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111741

Ekta Daweshar , Pankaj , Rajvee Mewada, Suveen Kumar

Oral carcinoma ranks sixth among the most fatal carcinomas, projecting a threat to the entire world, affecting the head and neck region. It is predominant in men due to several causative agents like tobacco, drinking, and HPV (human papillomavirus). Despite various diagnostic methods and treatments available, high rates of morbidity and mortality pose a significant challenge for oncologists toward its early detection. Conventional methods of diagnosis include biopsy, histopathology, and ELISA, which are time-consuming and non-compliant towards the patient. Therefore, developing an accurate, sensitive, selective, and cost-effective biosensing technology is imperative for timely prognosis, diagnosis, and treatment. Various electrochemical biosensors have been developed to ensure rapid and non-invasive /minimally invasive diagnosis of oral carcinoma. Nanomaterials have been extensively researched and utilized to develop invasive, minimally invasive, and non-invasive platforms for biosensor fabrication in oral carcinoma detection. This review aims to summarize various electrochemical biosensors developed for detecting oral carcinoma including the advantages and limitations of nanomaterials (metals, metal oxides and hydroxides, carbon, etc.), and other nanocomposite materials based on electrochemical biosensors. Finally, it also addresses the current challenges and prospects for developing handheld devices for oral carcinoma detection.

口腔癌在最致命的癌症中排名第六，对全世界都构成威胁，主要影响头颈部。由于烟草、饮酒和人乳头状瘤病毒（HPV）等多种致病因素，口腔癌主要发生在男性身上。尽管有各种诊断方法和治疗手段，但高发病率和高死亡率给肿瘤学家早期发现该病带来了巨大挑战。传统的诊断方法包括活组织切片检查、组织病理学检查和酶联免疫吸附试验（ELISA），这些方法既耗时又不符合患者的要求。因此，开发一种准确、灵敏、选择性强、成本效益高的生物传感技术对于及时预后、诊断和治疗至关重要。目前已开发出各种电化学生物传感器，以确保对口腔癌进行快速、无创/微创诊断。纳米材料已被广泛研究和利用，以开发用于口腔癌检测的有创、微创和无创生物传感器制造平台。本综述旨在总结为检测口腔癌而开发的各种电化学生物传感器，包括基于电化学生物传感器的纳米材料（金属、金属氧化物和氢氧化物、碳等）和其他纳米复合材料的优势和局限性。最后，报告还探讨了目前开发用于检测口腔癌的手持设备所面临的挑战和前景。

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引用次数: 0

Entropy-driven DNA nanomachine with magnetic assistance manipulating the aggregation of Au nanoparticles for label-free photothermal Aptasensing 磁性辅助熵驱动 DNA 纳米机械操纵金纳米粒子的聚集，用于无标记光热 Aptasensing

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111751

Hongbo Li , Jingling Zhang , Ye Cao , Qin Xu , Jing Li

Photothermal sensing has attracted increasing attention as a fast and portable detection method. However, photothermal sensors with excellent comprehensive performance still face challenges. Herein, a unique photothermal biosensor for bisphenol A as a model target is successfully constructed based on aptamer recognition coupled with the programmable entropy-driven DNA nanomachine cascaded strand displacement manipulating the aggregation of gold nanoparticles. In this protocol, the exposed bases of two DNA probes released from the DNA amplification network can anchor gold nanoparticles by van der Waals attraction. This allows gold nanoparticles to resist aggregation induced by a certain concentration of sodium chloride. Based on this exclusive mechanism, target-induced visual detection can be achieved by reading temperature changes. The two probes that are fully utilized to improve the atomic economy of the reaction and enhance the detection sensitivity. Also, label-free gold nanoparticles save time in the fabrication of the photothermal sensor. Moreover, visualized photothermal sensors with multi-color gradients enable self-verification of detection results, increasing sensor reliability. In particular, the splitting mode of magnetic-assisted extraction gives the designed photothermal sensor excellent specificity, which can meet the needs of real sample detection with high background noise. Additionally, this programmable and robust photothermal sensor has advantages of portability, replicability, rapid response, and simple operation.

光热传感作为一种快速、便携的检测方法日益受到关注。然而，综合性能优异的光热传感器仍面临挑战。本文以双酚 A 为模型靶标，成功构建了一种独特的光热生物传感器，该传感器是基于适配体识别和可编程熵驱动的 DNA 纳米机器级联链位移操纵金纳米粒子聚集的方法。在该方案中，从 DNA 扩增网络中释放出来的两个 DNA 探针的裸露碱基可通过范德华吸引力锚定金纳米粒子。这使得金纳米粒子能够抵御一定浓度氯化钠诱导的聚集。基于这种独特的机制，可以通过读取温度变化实现目标诱导视觉检测。这两种探针的充分利用改善了反应的原子经济性，提高了检测灵敏度。同时，无标记金纳米粒子节省了光热传感器的制造时间。此外，具有多色梯度的可视化光热传感器可对检测结果进行自我验证，提高了传感器的可靠性。特别是磁辅助萃取的分裂模式使所设计的光热传感器具有出色的特异性，可满足高背景噪声下实际样品检测的需要。此外，这种可编程、坚固耐用的光热传感器还具有便携、可复制、反应迅速、操作简单等优点。

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引用次数: 0

Early detection for carbohydrate antigen-19-9 based on surface enhanced Raman spectroscopy aptamer sensor 基于表面增强拉曼光谱适配体传感器的碳水化合物抗原-19-9 早期检测技术

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111750

Ji Xia , Yan Li , Yue Xin , Luyao Kang , Dan Lu

Background

Incipient screening for cervical cancer is crucial for treatment and improving prognosis. In this work, SERS based aptamer sensor (aptasensor) has been developed and applied to high-sensitivity detection of cervical cancer tumor marker (Carbohydrate antigen19-9 (CA19-9)) with a microporous chip, which was used as surface enhanced Raman scattering (SERS) analysis platform.

Methods

The aptamer of CA19-9 was immobilized on the gold nanodumbbell as a capture probe and the gold nanobipyramid containing complementary aptamer chains of the signal molecule Cy5 was modified as a signal probe. SERS aptasensor was constructed by combining signal probes and capture probes. It caused signal molecule Cy5 were sandwiched between the signal probes and capture probes; the constructed SERS aptasensor generated a strong SERS signal of Cy5. Based on the strong specificity of the aptamer, the CA19-9 aptamer binds specifically to CA19-9 when CA19-9 exist in the detection environment. The signal probe was released, resulting in a decrease in the SERS signal of the detection environment.

Results

Under the optimal detection conditions, the detection limit of the aptasensor was calculated to be 1.16 × 10⁻³ U/mL and limit of quantification is equal to 3.87 × 10⁻³ U/mL. In addition, a standard cervical cancer bearing mouse model was constructed and the SERS spectra of the serum of mice at different stages were measured. The CA19-9 content in the serum of mice at different stages was calculated. Compared with the results of enzyme-linked immunosorbent assay (ELISA), relative error (RE) values (2.54 %, −6.093 %, −4.922 %, and 3.04 %) of the CA19-9 concentrations detected by the two methods on the day of 0, 7, 14 and 28.

Conclusion

The proposed SERS aptasensor provides a new and reliable platform for early cervical cancer screening, and its low RE value proves that it will be a promising CA19-9 detection method.

背景宫颈癌的早期筛查对于治疗和改善预后至关重要。本研究开发了基于 SERS 的适配体传感器（aptasensor），并将其应用于宫颈癌肿瘤标志物（碳水化合物抗原 19-9 (CA19-9)）的高灵敏度检测，其微孔芯片被用作表面增强拉曼散射（SERS）分析平台。方法将 CA19-9 的适配体固定在金纳米哑铃上作为捕获探针，将含有信号分子 Cy5 的互补适配体链的金纳米双锥体修饰成信号探针。通过信号探针和捕获探针的组合，构建了 SERS 准传感器。信号分子Cy5被夹在信号探针和捕获探针之间，构建的SERS适配传感器产生了强烈的Cy5 SERS信号。基于适配体的强特异性，当CA19-9存在于检测环境中时，CA19-9适配体会与CA19-9发生特异性结合。结果在最佳检测条件下，计算出该适配体的检测限为 1.16 × 10-3 U/mL，定量限为 3.87 × 10-3 U/mL。此外，还构建了标准宫颈癌小鼠模型，并测定了不同阶段小鼠血清的 SERS 光谱。计算了不同阶段小鼠血清中 CA19-9 的含量。与酶联免疫吸附试验（ELISA）的结果相比，两种方法在0、7、14和28天检测到的CA19-9浓度的相对误差（RE）值分别为2.54%、-6.093%、-4.922%和3.04%。结论：所提出的SERS灵敏传感器为早期宫颈癌筛查提供了一个新的、可靠的平台，其较低的RE值证明它将是一种有前途的CA19-9检测方法。

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引用次数: 0

Determination of proteins in hypoxic Müller glia cells by single-cell ICP-MS: The use of a Rh-DNA intercalator for enhanced cell detection 利用单细胞 ICP-MS 测定缺氧 Müller 胶质细胞中的蛋白质：使用 Rh-DNA 中间体增强细胞检测能力

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111745

Alicia Villa-Vázquez , Paula Menero-Valdés , Lydia Álvarez , Héctor González-Iglesias , Beatriz Fernández , Rosario Pereiro

Single cell − inductively coupled plasma-mass spectrometry (sc-ICP-MS) enables the identification and quantification of elements and biomolecules within individual cells. Target proteins can be analyzed by sc-ICP-MS using metal nanoclusters (MNCs)-based immunoprobes, facilitating the detection of low amounts of proteins in each cell. However, for the full implementation of sc-ICP-MS, an effective strategy to enhance the detection of cellular events is required. In this work, the measurement of endogenous elements and two external tags (ruthenium red and Rh-DNA intercalator) was investigated for cell detection. As a proof of concept, the sequential determination of two proteins (hypoxia inducible factor-1α and vascular endothelial growth factor; HIF-1α and VEGF, respectively) in individual human Müller glia cells (MIO-M1) was pursued. Specific antibodies against HIF-1α and VEGF were labelled with AuNCs and IrNCs, respectively. The results demonstrated the advantages of using Rh-DNA intercalator for the detection and discrimination of cellular events by sc-ICP-MS for quadrupole (Q) mass spectrometers. Additionally, applying this analytical method to cultured MIO-M1 cells under hypoxic and to normoxic conditions showcased the ability of sc-ICP-QMS to study hypoxia-induced changes in the cell-to-cell distributions of HIF-1α and VEGF levels.

单细胞电感耦合等离子体质谱法（sc-ICP-MS）可对单个细胞内的元素和生物大分子进行鉴定和定量。利用基于金属纳米簇（MNCs）的免疫探针，sc-ICP-MS 可以分析目标蛋白质，从而有助于检测每个细胞中的低量蛋白质。然而，要全面实施 sc-ICP-MS，还需要一种有效的策略来加强对细胞事件的检测。在这项工作中，研究了如何测量内源元素和两种外部标记（钌红和 Rh-DNA 中间体）以进行细胞检测。作为概念验证，研究人员对单个人类 Müller 胶质细胞（MIO-M1）中的两种蛋白质（缺氧诱导因子-1α 和血管内皮生长因子；分别为 HIF-1α 和 VEGF）进行了连续测定。针对 HIF-1α 和 VEGF 的特异性抗体分别用 AuNCs 和 IrNCs 标记。结果表明，在四极杆（Q）质谱仪的 sc-ICP-MS 中使用 Rh-DNA 中间体检测和区分细胞事件具有优势。此外，将这种分析方法应用于缺氧和正常缺氧条件下培养的 MIO-M1 细胞，展示了 sc-ICP-QMS 研究缺氧诱导的细胞间 HIF-1α 和 VEGF 水平分布变化的能力。

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引用次数: 0

A highly sensitive sandwich-type electrochemical sensor for detection glypican-3 based on H-rGO-CMC@Pt NPs and aptamers 基于 H-rGO-CMC@Pt NPs 和适配体的高灵敏度夹心型电化学传感器，用于检测 glypican-3

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111747

Xiaohong Tan , Wenzhan Li , Wei He , Tingting Yu , Bingbing Wan , Yong Huang , Jintao Liang , Guiyin Li

Glypican-3 (GPC3), which is a surface heparan sulfate proteoglycan, has been widely known the ideal biomarker for diagnosis and treatment of hepatocellular carcinoma (HCC). Accurate and sensitive detection of serum GPC3 level is an important and challenging task. In this paper, a sandwich electrochemical aptasensor was designed to specifically detect serum GPC3 level through aptamer-target-aptamer recognition. A GPC3 aptamer (GPC3_Apt) immobilized on the surface of gold nanoparticles@reduced graphene oxide modified screen-printed electrode (Au NPs@rGO/SPCE) was used as the capture probe. Hemin-reduced graphene oxide-carboxymethyl chitosan@platinum nanoparticles (H-rGO-CMC@Pt NPs), with good mimicking peroxidases activity, were labeled with amination GPC3 aptamer, which was employed as the signal probe. Once the target GPC3 was anchored on the surface of GPC3_Apt/Au NPs@rGO/SPCE, the H-rGO-CMC@Pt NPs-GPC3_Apt was further specifically bound to the GPC3, forming the aptamer-target-aptamer sandwich structure, which can catalyze the oxidation of hydroquinone (HQ) to benzoquinone (BQ) and lead the oxidation current of HQ recorded by DPV to changing. The linear relationship between the current signal of oxidation of HQ and GPC3 concentration range of 0.0001–3.0 µg/mL and 3.0–60.0 µg/mL, with a low detection limit (LOD) of 0.0685 ng/mL. Additionally, the electrochemical aptasensor was successfully applied for the detection of GPC3 in human serum samples with the recovery of 99.95–104.06 % and relative standard deviation (RSD) of 1.31–5.22 %. Thus, the sandwich aptasensor could provide a new strategy for detection of serum GPC3 level and improve the early diagnosis rate of HCC.

Glypican-3（GPC3）是一种表面硫酸肝素蛋白多糖，已被广泛认为是诊断和治疗肝细胞癌（HCC）的理想生物标志物。准确而灵敏地检测血清 GPC3 水平是一项重要而具有挑战性的任务。本文设计了一种夹心电化学适配体传感器，通过适配体-目标-适配体的识别来特异性检测血清 GPC3 水平。GPC3 aptamer（GPC3Apt）被固定在金纳米粒子@还原氧化石墨烯修饰的丝网印刷电极（Au NPs@rGO/SPCE）表面作为捕获探针。血红素还原氧化石墨烯-羧甲基壳聚糖@铂纳米粒子（H-rGO-CMC@Pt NPs）具有良好的模拟过氧化物酶活性，其上标记了氨基化 GPC3 合酶，作为信号探针。目标 GPC3 被锚定在 GPC3Apt/Au NPs@rGO/SPCE 表面后，H-rGO-CMC@Pt NPs-GPC3Apt 进一步与 GPC3 特异性结合，形成aptamer-目标-aptamer 的三明治结构，从而催化对苯二酚（HQ）氧化成苯醌（BQ），并导致 DPV 记录的 HQ 氧化电流发生变化。HQ 氧化电流信号与 GPC3 浓度的线性关系范围分别为 0.0001-3.0 µg/mL 和 3.0-60.0 µg/mL，低检测限为 0.0685 ng/mL。此外，该电化学适配传感器还成功用于检测人血清样品中的 GPC3，回收率为 99.95-104.06%，相对标准偏差（RSD）为 1.31-5.22%。因此，夹心式灵敏传感器可为血清 GPC3 水平的检测提供一种新策略，并提高 HCC 的早期诊断率。

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引用次数: 0

Nanozymes in environmental remediation: A bibliometric and comprehensive review of their oxidoreductase-mimicking capabilities 环境修复中的纳米酶：关于氧化还原酶模拟能力的文献计量和综合评述

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111748

Vasundra Nagendran , Louella Concepta Goveas , Ramesh Vinayagam , Thivaharan Varadavenkatesan , Raja Selvaraj

Nanozymes mimic enzyme-like properties and possess unique characteristics inherent to nanomaterials, combining catalytic functionality with distinct nanostructure features. This class of emerging materials has garnered significant attention for their potential applications in environmental remediation. A bibliometric analysis was conducted to map research trends and identify key areas of focus in this rapidly evolving field. This review comprehensively examines the types and catalytic mechanisms of oxidoreductase nanozymes, and their diverse environmental applications with specific attention to detection and degradation of various pollutants such as dyes, pesticides, antibiotics and toxic ions. The intricate mechanisms of oxidoreductases mimicked by nanozymes and their roles in catalysing reactions for environmental remediation are discussed. Furthermore, their versatility and efficacy in addressing environmental challenges are elaborated with recent literature. Through a thorough analysis of recent advancements and case studies, this review provides valuable insights into the potential of nanozymes as efficient tools for sustainable environmental management.

纳米酶模仿酶的特性，具有纳米材料固有的独特特征，将催化功能与独特的纳米结构特征相结合。这一类新兴材料因其在环境修复方面的潜在应用而备受关注。我们进行了文献计量分析，以绘制研究趋势图，并确定这一快速发展领域的重点领域。本综述全面研究了氧化还原酶纳米分子的类型和催化机制，以及它们在环境中的各种应用，特别关注各种污染物（如染料、杀虫剂、抗生素和有毒离子）的检测和降解。讨论了纳米酶模仿氧化还原酶的复杂机制及其在环境修复催化反应中的作用。此外，还结合最新文献阐述了它们在应对环境挑战方面的多功能性和功效。通过对最新进展和案例研究的透彻分析，本综述为了解纳米微生物作为可持续环境管理有效工具的潜力提供了宝贵的见解。

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引用次数: 0