Microchemical Journal最新文献

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The label- and laser-free autocatalytic nucleic acid amplification reaction for the sensitive miRNA detection

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-08 DOI: 10.1016/j.microc.2025.113253

Siyuan Wang , Bingyue Zhao , Min Shang , Yaqi Liu , Hanghang Liu , Jiabing Ran , Zhangshuang Deng , Changying Yang , Huimin Wang

MicroRNAs (miRNAs) are increasingly recognized as vital biomarkers for cancer, given their aberrant expression patterns in oncogenesis. Sensitive detection of these miRNAs is imperative for early-stage cancer diagnostics. Current fluorescence-based assays, while effective, are limited by the need for costly labeling and external laser sources, contributing to elevated autofluorescence background signals. Our study introduces a label- and laser-free, chemiluminescence-based, autocatalytic DNA circuit composed of catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR), offering a sensitive and accurate approach to miRNA detection. The target T initiates CHA, generating multiple dsDNA products with exposed initiator I sequences. I triggers HCR to form long nanowires encoded with intact T and hemin/G-quadruplex DNAzyme structures, respectively, leading to self-catalytic cycles and amplified chemiluminescence signal output. By integrating auxiliary recognition hairpin, this autocatalytic chemiluminescence method enables the sensitive detection of miRNA. Overall, our sensitive and accurate miRNA assay provides a novel tool for the early diagnosis of cancer.

{"title":"The label- and laser-free autocatalytic nucleic acid amplification reaction for the sensitive miRNA detection","authors":"Siyuan Wang , Bingyue Zhao , Min Shang , Yaqi Liu , Hanghang Liu , Jiabing Ran , Zhangshuang Deng , Changying Yang , Huimin Wang","doi":"10.1016/j.microc.2025.113253","DOIUrl":"10.1016/j.microc.2025.113253","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are increasingly recognized as vital biomarkers for cancer, given their aberrant expression patterns in oncogenesis. Sensitive detection of these miRNAs is imperative for early-stage cancer diagnostics. Current fluorescence-based assays, while effective, are limited by the need for costly labeling and external laser sources, contributing to elevated autofluorescence background signals. Our study introduces a label- and laser-free, chemiluminescence-based, autocatalytic DNA circuit composed of catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR), offering a sensitive and accurate approach to miRNA detection. The target T initiates CHA, generating multiple dsDNA products with exposed initiator I sequences. I triggers HCR to form long nanowires encoded with intact T and hemin/G-quadruplex DNAzyme structures, respectively, leading to self-catalytic cycles and amplified chemiluminescence signal output. By integrating auxiliary recognition hairpin, this autocatalytic chemiluminescence method enables the sensitive detection of miRNA. Overall, our sensitive and accurate miRNA assay provides a novel tool for the early diagnosis of cancer.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113253"},"PeriodicalIF":4.9,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of stable thiol-functionalized magnetic nanoparticles for magnetic solid phase extraction of As(III) in environmental waters

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113268

Mianzhi Wang, Man He, Beibei Chen, Bin Hu

Thiol-based materials exhibit good adsorption properties for sulfophilic elements, such as As(III). However, these materials are prone to oxidation in air, leading to poor storage stability, which limits their practical application. To address this problem, using cystamine dihydrochloride (CA) as the functional reagent and glycidyl methacrylate (GMA) modified magnetic nanoparticles (MNPs) as the core, MNPs of Fe₃O₄@SiO₂@GMA-CA was synthesized via an amino-epoxy click chemistry reaction, and the thiol-functionalized MNPs of Fe₃O₄@SiO₂@GMA-CA-SH was obtained after breaking the disulfide bond of CA. The material exhibits great storage stability when preserved in the form of Fe₃O₄@SiO₂@GMA-CA, and the Fe₃O₄@SiO₂@GMA-CA-SH possesses strong magnetism and high adsorption selectivity for As(III). With the use of Fe₃O₄@SiO₂@GMA-CA-SH as the adsorbent, a new method of magnetic solid phase extraction (MSPE) combined with inductively coupled plasma optical emission spectrometry (ICP-OES) was established for the speciation analysis of As(III) and As(V) in environmental water samples. Various experimental parameters affecting the MSPE have been optimized. Under the optimal conditions, the limit of detection (LOD) of the developed MSPE-ICP-OES method was 0.050 μg L⁻¹, the relative standard deviation was 2.8 %, and the enrichment factor was 150-fold. The accuracy of the method was validated through the analysis of the Certified Reference Materials of GBW08666 and GBW08667. The established method was successfully applied to the speciation analysis of inorganic arsenic in potential arsenic-contaminated environmental water samples, demonstrating a great application potential in real-world sample analysis.

{"title":"Preparation of stable thiol-functionalized magnetic nanoparticles for magnetic solid phase extraction of As(III) in environmental waters","authors":"Mianzhi Wang, Man He, Beibei Chen, Bin Hu","doi":"10.1016/j.microc.2025.113268","DOIUrl":"10.1016/j.microc.2025.113268","url":null,"abstract":"<div><div>Thiol-based materials exhibit good adsorption properties for sulfophilic elements, such as As(III). However, these materials are prone to oxidation in air, leading to poor storage stability, which limits their practical application. To address this problem, using cystamine dihydrochloride (CA) as the functional reagent and glycidyl methacrylate (GMA) modified magnetic nanoparticles (MNPs) as the core, MNPs of Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@GMA-CA was synthesized via an amino-epoxy click chemistry reaction, and the thiol-functionalized MNPs of Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@GMA-CA-SH was obtained after breaking the disulfide bond of CA. The material exhibits great storage stability when preserved in the form of Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@GMA-CA, and the Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@GMA-CA-SH possesses strong magnetism and high adsorption selectivity for As(III). With the use of Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@GMA-CA-SH as the adsorbent, a new method of magnetic solid phase extraction (MSPE) combined with inductively coupled plasma optical emission spectrometry (ICP-OES) was established for the speciation analysis of As(III) and As(V) in environmental water samples. Various experimental parameters affecting the MSPE have been optimized. Under the optimal conditions, the limit of detection (LOD) of the developed MSPE-ICP-OES method was 0.050 μg L<sup>−1</sup>, the relative standard deviation was 2.8 %, and the enrichment factor was 150-fold. The accuracy of the method was validated through the analysis of the Certified Reference Materials of GBW08666 and GBW08667. The established method was successfully applied to the speciation analysis of inorganic arsenic in potential arsenic-contaminated environmental water samples, demonstrating a great application potential in real-world sample analysis.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113268"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of different solvents on biological attributes of olive leaves extracts of newly cultivated varieties:GC–MS based investigations

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113281

Sami Ullah , Muhammad Azhar Iqbal

This study investigates the optimal solvents for extraction of bioactives from olive leaves, analyzing the yield, phenolic and flavonoid content, antioxidant, thrombolytic, antimicrobial activities, and chemical profiles of extracts from three olive varieties: Kalamata, Sikitita, and Chietina. During ultrasonic-assisted extraction, the highest extract yield (472.96 mg/g) was achieved with water, while the lowest (224.31 mg/g) antioxidant extract yield was obtained with absolute methanol. On the other hand, aqueous ethanolic extracts (AEEs) demonstrated the highest total phenolics (28.17 mg GAE/g DW) and flavonoids (170.67 mg RE/g DW) contents, along with significant antioxidant activities as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays. Gas chromatography–mass spectrometry (GC–MS) analysis of these extracts revealed the presence of high-valuebioactive compounds such as oleic acid, β-sitosterol, and 2-methoxy-4-vinylphenol, known for their health benefits. Moreover, hemolytic and thrombolytic activities revealed the safer and health-promoting potential of tested extracts. Carbohydrate analysis identified glucose as the most abundant sugar, while organic acid profiling highlighted tartaric acid as predominant. The AEEs extracts were the most effective against Gram-positive pathogens, particularly Staphylococcus aureus, but were less effective against Gram-negative bacteria. These findings highlight the importance of solvent choice in maximizing the extraction of bioactives and suggest the potential of olive leaf extracts for developing nutraceuticals and functional foods.

{"title":"Effect of different solvents on biological attributes of olive leaves extracts of newly cultivated varieties:GC–MS based investigations","authors":"Sami Ullah , Muhammad Azhar Iqbal","doi":"10.1016/j.microc.2025.113281","DOIUrl":"10.1016/j.microc.2025.113281","url":null,"abstract":"<div><div>This study investigates the optimal solvents for extraction of bioactives from olive leaves, analyzing the yield, phenolic and flavonoid content, antioxidant, thrombolytic, antimicrobial activities, and chemical profiles of extracts from three olive varieties: Kalamata, Sikitita, and Chietina. During ultrasonic-assisted extraction, the highest extract yield (472.96 mg/g) was achieved with water, while the lowest (224.31 mg/g) antioxidant extract yield was obtained with absolute methanol. On the other hand, aqueous ethanolic extracts (AEEs) demonstrated the highest total phenolics (28.17 mg GAE/g DW) and flavonoids (170.67 mg RE/g DW) contents, along with significant antioxidant activities as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays. Gas chromatography–mass spectrometry (GC–MS) analysis of these extracts revealed the presence of high-valuebioactive compounds such as oleic acid, <em>β</em>-sitosterol, and 2-methoxy-4-vinylphenol, known for their health benefits. Moreover, hemolytic and thrombolytic activities revealed the safer and health-promoting potential of tested extracts. Carbohydrate analysis identified glucose as the most abundant sugar, while organic acid profiling highlighted tartaric acid as predominant. The AEEs extracts were the most effective against Gram-positive pathogens, particularly <em>Staphylococcus aureus</em>, but were less effective against Gram-negative bacteria. These findings highlight the importance of solvent choice in maximizing the extraction of bioactives and suggest the potential of olive leaf extracts for developing nutraceuticals and functional foods.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113281"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ratiometric colorimetric and fluorometric dual-sensing system based on anthracene derivatives for the determination of ClO− and total antioxidant capacity

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113234

Yan Zhang , Binling Yan , Bing Liu , Yuting Wang , Wenjing Qi , Lianzhe Hu , Jiaxing Song

In this work, a fluorescent small molecule based on 9,10-distyryl anthracene (DSA-2OH) with AIE properties was prepared. DSA-2OH can be oxidized by hypochlorite (ClO⁻) to oxidized DSA (ODSA), which can be re-reduced to DSA-2OH by reducing agents. This oxidation–reduction process is accompanied by changes in fluorescence intensity and absorption wavelength. Based on this principle, a colorimetric/fluorometric dual-mode assay for the determination of ClO⁻ and the total antioxidant capacity (TAC) are realized. In contrast to the colorimetric method, the fluorometric method has better detection effect. The fluorometric detection method can detect ClO⁻ and ascorbic acid (AA) in a three-order-of-magnitude concentration range (0.1–20 μM and 0.2–30 μM). The detection limits (LOD) of fluorometric methods for ClO⁻ and AA are 44 nM and 55 nM, respectively. The two methods can mutually validate each other to improve detection accuracy. The proposed assay can detect TAC in drinks such as orange juice, kiwi juice and drinking water with recoveries ranging from 95 % to 105 %, which can help us to make healthier and more sensible dietary choices.

{"title":"Ratiometric colorimetric and fluorometric dual-sensing system based on anthracene derivatives for the determination of ClO− and total antioxidant capacity","authors":"Yan Zhang , Binling Yan , Bing Liu , Yuting Wang , Wenjing Qi , Lianzhe Hu , Jiaxing Song","doi":"10.1016/j.microc.2025.113234","DOIUrl":"10.1016/j.microc.2025.113234","url":null,"abstract":"<div><div>In this work, a fluorescent small molecule based on 9,10-distyryl anthracene (DSA-2OH) with AIE properties was prepared. DSA-2OH can be oxidized by hypochlorite (ClO<sup>−</sup>) to oxidized DSA (ODSA), which can be re-reduced to DSA-2OH by reducing agents. This oxidation–reduction process is accompanied by changes in fluorescence intensity and absorption wavelength. Based on this principle, a colorimetric/fluorometric dual-mode assay for the determination of ClO<sup>−</sup> and the total antioxidant capacity (TAC) are realized. In contrast to the colorimetric method, the fluorometric method has better detection effect. The fluorometric detection method can detect ClO<sup>−</sup> and ascorbic acid (AA) in a three-order-of-magnitude concentration range (0.1–20 μM and 0.2–30 μM). The detection limits (LOD) of fluorometric methods for ClO<sup>−</sup> and AA are 44 nM and 55 nM, respectively. The two methods can mutually validate each other to improve detection accuracy. The proposed assay can detect TAC in drinks such as orange juice, kiwi juice and drinking water with recoveries ranging from 95 % to 105 %, which can help us to make healthier and more sensible dietary choices.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113234"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly sensitive and anti-interference Bio-QDs biosensor for electrochemical detection calcium ion in plant tissue culture medium

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113284

Jie Cui , Yuxin Xia , Leilei Jin , Jishuang Chen , Zongli Huo , Fei Shen , Li Mi

Metal ions are widely present in biological systems and participate in many critical biochemical processes. Resisting interfering ions and achieving specific detection at the biosensor present a significant challenge. Herein, we designed a highly sensitive and anti-interference Bio-QDs biosensor through the biosynthesis CdSe quantum dots (QDs) and the use of sodium hexametaphosphate (HMP) as a stabilizer (CdSe QD@HMP) for detecting the calcium ion (Ca²⁺) in plant tissue culture medium, which is known for its complexity. Because of the spatial hexahedron molecular structure and six interconnected tetrahedral P-O units of HMP increased the electronegativity and stability of CdSe, the CdSe QD@HMP easily captured Ca²⁺ in solution, resulting in a minimum detection limit of 0.377 µM for Ca²⁺ in the range of 1–300 μM. And HMP can also ensure that the detection process is not affected by negative ion interference. Additionally, the recovery rate of environmental samples was 96.40 % and 101.13 %, confirming the reliability and practicality of the prepared biosensors. Therefore, this strategy not only provides a green and safe pathway for synthesizing QDs, but also lays the foundation for the sensitive determination of metal cations in complex environment.

{"title":"A highly sensitive and anti-interference Bio-QDs biosensor for electrochemical detection calcium ion in plant tissue culture medium","authors":"Jie Cui , Yuxin Xia , Leilei Jin , Jishuang Chen , Zongli Huo , Fei Shen , Li Mi","doi":"10.1016/j.microc.2025.113284","DOIUrl":"10.1016/j.microc.2025.113284","url":null,"abstract":"<div><div>Metal ions are widely present in biological systems and participate in many critical biochemical processes. Resisting interfering ions and achieving specific detection at the biosensor present a significant challenge. Herein, we designed a highly sensitive and anti-interference Bio-QDs biosensor through the biosynthesis CdSe quantum dots (QDs) and the use of sodium hexametaphosphate (HMP) as a stabilizer (CdSe QD@HMP) for detecting the calcium ion (Ca<sup>2+</sup>) in plant tissue culture medium, which is known for its complexity. Because of the spatial hexahedron molecular structure and six interconnected tetrahedral P-O units of HMP increased the electronegativity and stability of CdSe, the CdSe QD@HMP easily captured Ca<sup>2+</sup> in solution, resulting in a minimum detection limit of 0.377 µM for Ca<sup>2+</sup> in the range of 1–300 μM. And HMP can also ensure that the detection process is not affected by negative ion interference. Additionally, the recovery rate of environmental samples was 96.40 % and 101.13 %, confirming the reliability and practicality of the prepared biosensors. Therefore, this strategy not only provides a green and safe pathway for synthesizing QDs, but also lays the foundation for the sensitive determination of metal cations in complex environment.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113284"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of linalool by QCM gas sensor based on HKUST-1/MWCNT-gel@MIP in early sweetpotato black spot disease

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113232

Suqi Liu , Xiaowei Wu , Shihao Chen , Cong Liu , Wanqi Zhang , Mingyi Yang , Jiyu Cheng , Guoquan Lu , Zhenhe Wang , Wei Chen , Yuge Guan , Linjiang Pang , Xinghua Lu

Linalool, a terpenoid alcohol with bacteriostatic properties, was detected in sweetpotato suffering from black spot disease. In this research, a composite material based on HKUST-1/MWCNT-gel@MIP was employed to detect the linalool content in these infected sweetpotatoes. The micro-morphology and crystal structure of the composite were characterized using scanning electron microscopy (SEM), fourier transform infrared (FTIR), and x-ray diffraction (XRD). Experimental results demonstrated that the quartz crystal microbalance (QCM) gas sensor of HKUST-1/MWCNT-gel@MIP composite exhibited high sensitivity (0.873 Hz/ppm), a low detection limit (0.237 ppm), a small standard deviation (0.83 %), good reproducibility, and outstanding selectivity. The QCM gas sensor data from actual samples showed a strong correlation with gas chromatography-mass spectrometry (GC–MS) results (r = −0.976, p < 0.01), demonstrating its exceptional ability to identify linalool. Thus, it can be used for early detection of sweetpotato black spot disease.

{"title":"Rapid detection of linalool by QCM gas sensor based on HKUST-1/MWCNT-gel@MIP in early sweetpotato black spot disease","authors":"Suqi Liu , Xiaowei Wu , Shihao Chen , Cong Liu , Wanqi Zhang , Mingyi Yang , Jiyu Cheng , Guoquan Lu , Zhenhe Wang , Wei Chen , Yuge Guan , Linjiang Pang , Xinghua Lu","doi":"10.1016/j.microc.2025.113232","DOIUrl":"10.1016/j.microc.2025.113232","url":null,"abstract":"<div><div>Linalool, a terpenoid alcohol with bacteriostatic properties, was detected in sweetpotato suffering from black spot disease. In this research, a composite material based on HKUST-1/MWCNT-gel@MIP was employed to detect the linalool content in these infected sweetpotatoes. The micro-morphology and crystal structure of the composite were characterized using scanning electron microscopy (SEM), fourier transform infrared (FTIR), and x-ray diffraction (XRD). Experimental results demonstrated that the quartz crystal microbalance (QCM) gas sensor of HKUST-1/MWCNT-gel@MIP composite exhibited high sensitivity (0.873 Hz/ppm), a low detection limit (0.237 ppm), a small standard deviation (0.83 %), good reproducibility, and outstanding selectivity. The QCM gas sensor data from actual samples showed a strong correlation with gas chromatography-mass spectrometry (GC–MS) results (r = −0.976, p < 0.01), demonstrating its exceptional ability to identify linalool. Thus, it can be used for early detection of sweetpotato black spot disease.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113232"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multienzyme isothermal rapid amplification combined with CRISPR/Cas12a for sensitive prostate cancer screening using urine collected without digital rectal examination

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113218

Hong Chen , Jiakai Liu , Yongyi Chen , Zhen Song , Jichun Shao , Bo Shi , Yang He

Prostate cancer associated 3 (PCA3), a specific nucleic acid biomarker of prostate cancer, may appear earlier than the prostate-specific antigen (PSA) in prostate cancer. At present, urine, to be used for PCA3 detection, is collected after digital rectal examination (DRE). However, omitting DRE without negatively affecting assay sensitivity may help standardize urine collection procedures and increase PCA3 diagnostic applicability. Based on the DNA replication function of multienzyme isothermal rapid amplification (MIRA) and the specific DNA-shearing function of CRISPR/Cas12a, the MIRA-CRISPR/Cas12a “one-pot” system was developed. The system was shown to detect 0.01 ng/µL RNA in 40 min at room temperature. In addition, this “one-pot” ambient temperature reaction system reduced aerosol contamination and reliance on expensive equipment. Of note, the assay results could be obtained with the naked eye under ultraviolet light. Finally, PCA3 detection results revealed that the marker had great potential for prostate cancer screening, with a specificity of 83.3 %. Compared with the 33.3 % specificity of serum PSA, the specificity of PCA3 for prostate cancer screening was increased by approximately 50 %.

{"title":"Multienzyme isothermal rapid amplification combined with CRISPR/Cas12a for sensitive prostate cancer screening using urine collected without digital rectal examination","authors":"Hong Chen , Jiakai Liu , Yongyi Chen , Zhen Song , Jichun Shao , Bo Shi , Yang He","doi":"10.1016/j.microc.2025.113218","DOIUrl":"10.1016/j.microc.2025.113218","url":null,"abstract":"<div><div>Prostate cancer associated 3 (<em>PCA3</em>), a specific nucleic acid biomarker of prostate cancer, may appear earlier than the prostate-specific antigen (PSA) in prostate cancer. At present, urine, to be used for <em>PCA3</em> detection, is collected after digital rectal examination (DRE). However, omitting DRE without negatively affecting assay sensitivity may help standardize urine collection procedures and increase <em>PCA3</em> diagnostic applicability. Based on the DNA replication function of multienzyme isothermal rapid amplification (MIRA) and the specific DNA-shearing function of CRISPR/Cas12a, the MIRA-CRISPR/Cas12a “one-pot” system was developed. The system was shown to detect 0.01 ng/µL RNA in 40 min at room temperature. In addition, this “one-pot” ambient temperature reaction system reduced aerosol contamination and reliance on expensive equipment. Of note, the assay results could be obtained with the naked eye under ultraviolet light. Finally, <em>PCA3</em> detection results revealed that the marker had great potential for prostate cancer screening, with a specificity of 83.3 %. Compared with the 33.3 % specificity of serum PSA, the specificity of <em>PCA3</em> for prostate cancer screening was increased by approximately 50 %.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113218"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of amino resin solids content with PLS based on NIR: Improving model performance using a data balancing strategy

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113279

Roberto Magalhães , Nádia T. Paiva , Fernão D. Magalhães , F.G. Martins

Monitoring the solids content (SC) of amino resins plays a significant role in reducing costs and increasing production efficiency in the wood-based panels (WBP) industry. The goal of this study was to use NIR spectroscopy and regression-based methods for predicting SC in amino resins. Several wavenumber intervals were investigated to determine the best regions of the spectrum for model improvement. To address dataset imbalances, an oversampling technique was used, resulting in a more accurate representation of underrepresented SC values in the initial dataset.

The calibration and test set splitting were performed using the random (RD) method, as well as the Sample set Partitioning based on joint X–Y distances (SPXY) and Kennard-Stone (KS) methods, which improved model reliability by providing calibration data that covered the entire input space. Predictive models were developed using Partial Least Squares (PLS) regression, with the number of latent variables optimized through 10-fold cross-validation. Combining wavenumber interval selection, oversampling, and the KS split enabled significantly improved prediction performance metrics and robustness. This approach provides an effective alternative for the WBP industry, allowing for more efficient and robust quality control of amino resins. The best model had a maximum absolute error of 0.3 % on the test set, which was comparable to the performance of the reference method, demonstrating its potential for use in industrial applications.

{"title":"Prediction of amino resin solids content with PLS based on NIR: Improving model performance using a data balancing strategy","authors":"Roberto Magalhães , Nádia T. Paiva , Fernão D. Magalhães , F.G. Martins","doi":"10.1016/j.microc.2025.113279","DOIUrl":"10.1016/j.microc.2025.113279","url":null,"abstract":"<div><div>Monitoring the solids content (SC) of amino resins plays a significant role in reducing costs and increasing production efficiency in the wood-based panels (WBP) industry. The goal of this study was to use NIR spectroscopy and regression-based methods for predicting SC in amino resins. Several wavenumber intervals were investigated to determine the best regions of the spectrum for model improvement. To address dataset imbalances, an oversampling technique was used, resulting in a more accurate representation of underrepresented SC values in the initial dataset.</div><div>The calibration and test set splitting were performed using the random (RD) method, as well as the Sample set Partitioning based on joint X–Y distances (SPXY) and Kennard-Stone (KS) methods, which improved model reliability by providing calibration data that covered the entire input space. Predictive models were developed using Partial Least Squares (PLS) regression, with the number of latent variables optimized through 10-fold cross-validation. Combining wavenumber interval selection, oversampling, and the KS split enabled significantly improved prediction performance metrics and robustness. This approach provides an effective alternative for the WBP industry, allowing for more efficient and robust quality control of amino resins. The best model had a maximum absolute error of 0.3 % on the test set, which was comparable to the performance of the reference method, demonstrating its potential for use in industrial applications.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113279"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced CuMnCr-LDH modified glassy carbon electrode for simultaneous electrochemical quantification of cabotegravir and paracetamol in biological and pharmaceutical samples

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113267

Wiem Bouali , Asena Ayse Genc , Nevin Erk , Hassan Elzain Hassan Ahmed , Mustafa Soylak

The simultaneous detection of antiviral and analgesic drugs in biological samples is critical for effective drug therapy monitoring. In this study, a novel electrochemical sensor was developed for the concurrent determination of the antiviral drug Cabotegravir (CABO) and the analgesic Paracetamol (PARA). The sensor was fabricated by modifying a glassy carbon electrode (GCE) with a CuMnCr-LDH nanocomposite, which served as an efficient electrocatalyst. The electrochemical properties of the modified electrode were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The novel CuMnCr-LDH composite exhibits exceptional electrocatalytic activity due to its numerous active sites, reduced charge-transfer resistance, and rapid electron transfer. The sensor’s performance for simultaneous CABO and PARA detection was assessed using CV and differential pulse voltammetry (DPV) techniques. Under optimized conditions, the sensor demonstrated excellent sensitivity with linear ranges of 0.06–28.3 μM for CABO and 0.4–53 μM for PARA and detection limits (LOD) of 0.028 μM and 0.19 μM, respectively. Furthermore, the sensor demonstrated excellent reliability and accuracy when applied to pharmaceutical and biological samples, showcasing its potential for real-world applications in clinical and pharmaceutical settings.

{"title":"Advanced CuMnCr-LDH modified glassy carbon electrode for simultaneous electrochemical quantification of cabotegravir and paracetamol in biological and pharmaceutical samples","authors":"Wiem Bouali , Asena Ayse Genc , Nevin Erk , Hassan Elzain Hassan Ahmed , Mustafa Soylak","doi":"10.1016/j.microc.2025.113267","DOIUrl":"10.1016/j.microc.2025.113267","url":null,"abstract":"<div><div>The simultaneous detection of antiviral and analgesic drugs in biological samples is critical for effective drug therapy monitoring. In this study, a novel electrochemical sensor was developed for the concurrent determination of the antiviral drug Cabotegravir (CABO) and the analgesic Paracetamol (PARA). The sensor was fabricated by modifying a glassy carbon electrode (GCE) with a CuMnCr-LDH nanocomposite, which served as an efficient electrocatalyst. The electrochemical properties of the modified electrode were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The novel CuMnCr-LDH composite exhibits exceptional electrocatalytic activity due to its numerous active sites, reduced charge-transfer resistance, and rapid electron transfer. The sensor’s performance for simultaneous CABO and PARA detection was assessed using CV and differential pulse voltammetry (DPV) techniques. Under optimized conditions, the sensor demonstrated excellent sensitivity with linear ranges of 0.06–28.3 μM for CABO and 0.4–53 μM for PARA and detection limits (LOD) of 0.028 μM and 0.19 μM, respectively. Furthermore, the sensor demonstrated excellent reliability and accuracy when applied to pharmaceutical and biological samples, showcasing its potential for real-world applications in clinical and pharmaceutical settings.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113267"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanozyme-assisted CRISPR/Cas systems as an emerging platform for food safety applications: Recent advances

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-06 DOI: 10.1016/j.microc.2025.113270

Esmaeil Babaei , Shukur Wasman smail , Salah Tofik Jalal Balaky , Noor Mohsin Hadi , Hewa Jalal Azeez

It is of paramount importance to establish user-friendly, consensus-driven, and conceptually novel techniques for ensuring food safety. The CRISPR/Cas system represents an emerging technology with exceptional capabilities for targeting RNA and DNA. It has been repurposed as a robust tool for the diagnosis of nucleic acids. Nevertheless, most CRISPR-based techniques rely on specialized detection equipment and expensive reporters, despite their exceptional accuracy and rapid analysis capabilities. Recently, nanozymes have been integrated into CRISPR/Cas-based biosensors due to their low cost, good stability, higher recycling efficiency, simpler preparation processes, and more robust catalytic functions. Therefore, significant efforts have been devoted to the advancement of nanozyme-integrated CRISPR/Cas-derived biosensors for the analysis of various targets. This review focuses on the recent developments in nanozyme-assisted CRISPR/Cas systems-based biosensors, with particular emphasis on the detection principles and performance of these platforms. The principles and properties of commonly employed CRISPR/Cas systems are outlined. Additionally, the application of nanozyme-assisted CRISPR/Cas systems in food safety analysis, which includes pathogenic bacteria, viruses, toxins, and heavy metal ions, is thoroughly described. Ultimately, the main challenges and future trends in this field are discussed in detail.

{"title":"Nanozyme-assisted CRISPR/Cas systems as an emerging platform for food safety applications: Recent advances","authors":"Esmaeil Babaei , Shukur Wasman smail , Salah Tofik Jalal Balaky , Noor Mohsin Hadi , Hewa Jalal Azeez","doi":"10.1016/j.microc.2025.113270","DOIUrl":"10.1016/j.microc.2025.113270","url":null,"abstract":"<div><div>It is of paramount importance to establish user-friendly, consensus-driven, and conceptually novel techniques for ensuring food safety. The CRISPR/Cas system represents an emerging technology with exceptional capabilities for targeting RNA and DNA. It has been repurposed as a robust tool for the diagnosis of nucleic acids. Nevertheless, most CRISPR-based techniques rely on specialized detection equipment and expensive reporters, despite their exceptional accuracy and rapid analysis capabilities. Recently, nanozymes have been integrated into CRISPR/Cas-based biosensors due to their low cost, good stability, higher recycling efficiency, simpler preparation processes, and more robust catalytic functions. Therefore, significant efforts have been devoted to the advancement of nanozyme-integrated CRISPR/Cas-derived biosensors for the analysis of various targets. This review focuses on the recent developments in nanozyme-assisted CRISPR/Cas systems-based biosensors, with particular emphasis on the detection principles and performance of these platforms. The principles and properties of commonly employed CRISPR/Cas systems are outlined. Additionally, the application of nanozyme-assisted CRISPR/Cas systems in food safety analysis, which includes pathogenic bacteria, viruses, toxins, and heavy metal ions, is thoroughly described. Ultimately, the main challenges and future trends in this field are discussed in detail.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113270"},"PeriodicalIF":4.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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