Microchemical Journal最新文献_第6页

Machine learning-enabled FTIR spectroscopy for discriminating Gentiana rigescens habitat characteristics under current climate 基于机器学习的FTIR光谱识别当前气候下龙胆栖息地特征

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-06 DOI: 10.1016/j.microc.2026.117299

Gang He , Tao Shen , Yuan-zhong Wang

Gentiana rigescens Franch. ex Hemsl. (G. rigescens) is a well-known medicinal plant in China, which has been affected by environmental changes and human activities, and the quality of G. rigescens varies from different origins, which increases consumers' concerns. Therefore, this study employs Fourier Transform Infrared Spectroscopy (FTIR) combined with machine learning methods to construct a rapid, accurate, and comprehensive approach for the precise classification and identification of origin and plant part of G. rigescens. The results indicate that mean diurnal range, temperature seasonality, and elevation are significant factors contributing to the differences in the growth environments of G. rigescens. When using FTIR spectroscopy to identify the origin and parts of G. rigescens, the application of appropriate preprocessing (second derivative, SD) and feature extraction (Intersection) could effectively enhance the performance of the model. Transforming the FTIR spectral data preprocessed by the SD into two-dimensional correlation (2DCOS) images enabled the Residual Convolutional Neural Network (ResNet) to accurately identify the origin and parts of G. rigescens. The classification accuracy rates for the training set, testing set, and external validation set all reached 100.00%, with no risk of overfitting. The prediction results of the Maximum Entropy (MaxEnt) model indicated that the moderately to highly suitable habitats for this species were mainly distributed in the Yunnan-Guizhou Plateau and the Hengduan Mountains area in northwestern Yunnan. Conditions with small temperature variations and sufficient precipitation were conducive to the accumulation of the active ingredients in G. rigescens. This study can provide a reference for the scientific cultivation, quality evaluation, and resource conservation of G. rigescens.

龙胆Hemsl交货。（G. rigescens）是中国知名的药用植物，受到环境变化和人类活动的影响，不同产地的rigescens质量也不同，这增加了消费者的关注。因此，本研究采用傅里叶变换红外光谱（FTIR）技术结合机器学习方法，构建了一种快速、准确、全面的方法来精确分类和鉴定棘骨参的来源和植物部位。结果表明，平均日差、温度季节性和海拔高度是影响黄颡鱼生长环境差异的重要因素。在利用FTIR光谱技术识别龙参的来源和部位时，采用适当的预处理（二阶导数，SD）和特征提取（交集）可以有效地提高模型的性能。利用残差卷积神经网络（ResNet）将SD预处理后的FTIR光谱数据转化为二维相关（2DCOS）图像，可以准确识别出龙葵的来源和部位。训练集、测试集和外部验证集的分类准确率均达到100.00%，无过拟合风险。最大熵（MaxEnt）模型的预测结果表明，中高适宜生境主要分布在云贵高原和滇西北横断山区。温度变化小、降水充足的条件有利于黄芪有效成分的积累。本研究可为黄芪的科学栽培、质量评价和资源保护提供参考。

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引用次数: 0

Enhanced dual-mode nanozyme detection of multiple heavy metal ions enabled by the hydroxyl overflow effect 利用羟基溢出效应增强对多种重金属离子的双模纳米酶检测

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-07 DOI: 10.1016/j.microc.2026.117313

Yufei Zhong , Zhuqin Zhang , Quan Chen , Yu Sun , Guining Lu , Zhi Dang , Lijuan Zhang

Site contamination is often associated with the coexistence of multiple heavy metal ions, and their simultaneous detection remains highly challenging due to the distinct physicochemical properties of anionic and cationic species. In this study, we developed a multifunctional iron-modified mesoporous polydopamine nanozyme (MPNZ) capable of detecting both anionic Cr(VI) and cationic heavy metals, including Cd(II), Pb(II), Cu(II), and Hg(II). MPNZ exhibits peroxidase-like activity enabled by Fe(II)/Fe(III) redox cycling and a hydroxyl overflow effect, catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) for colorimetric detection of Cr(VI), with a detection limit of 60 nM. The mesoporous structure and abundant amino/imine groups facilitate strong metal ion coordination, allowing electrochemical detection of cationic species via differential pulse stripping voltammetry (DPSV). The platform achieves excellent sensitivity, with detection limits of 12 nM for Cd(II)), 3 nM for Pb(II), 1 nM for Cu(II), and 0.3 nM for Hg(II). Application to real samples demonstrated recovery rates of 90%–110%, confirming its practical utility. This work highlights the potential of MPNZ as a versatile sensing platform, advancing the application of nanozymes for the detection of both anionic and cationic heavy metals in environmental monitoring.

场地污染通常与多种重金属离子共存有关，由于阴离子和阳离子的物理化学性质不同，同时检测它们仍然是极具挑战性的。在这项研究中，我们开发了一种多功能铁修饰的介孔聚多巴胺纳米酶（MPNZ），能够检测阴离子Cr（VI）和阳离子重金属，包括Cd(II), Pb(II), Cu（II）和Hg（II）。MPNZ具有过氧化物酶样活性，通过Fe(II)/Fe（III）氧化还原循环和羟基溢出效应，催化3,3 ',5,5 ' -四甲基联苯胺（TMB）的氧化，用于比色检测Cr(VI)，检测限为60 nM。介孔结构和丰富的氨基/亚胺基促进了强金属离子配位，允许通过差分脉冲溶出伏安法（DPSV）进行阳离子的电化学检测。该平台灵敏度极高，Cd（II）的检出限为12 nM， Pb（II）的检出限为3 nM， Cu（II）的检出限为1 nM， Hg（II）的检出限为0.3 nM。实际样品的回收率为90% ~ 110%，验证了该方法的实用性。这项工作突出了MPNZ作为一个多功能传感平台的潜力，推进了纳米酶在环境监测中检测阴离子和阳离子重金属的应用。

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引用次数: 0

Detection of liver cancer serum markers and molecular analysis of metabolites based on electrochemical sensors: Combined targeted immunotherapy 基于电化学传感器的肝癌血清标志物检测及代谢物分子分析：联合靶向免疫治疗

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-06 DOI: 10.1016/j.microc.2026.117194

Yanhui Hu, Qingli Cui, Xu Sun, Dongyang Ma

This study aims to compare the clinical efficacy and safety of TACE-HAIC combined with targeted and immunotherapy (quadruple therapy) and TACE combined with targeted and immunotherapy (triple regimen) in advanced hepatocellular carcinoma (HCC). Although progress has been made in the current systemic and local combined treatment strategies for HCC, the dynamic and precise assessment of therapeutic efficacy still highly relies on traditional imaging and intermittent serum marker detection, which has limitations such as delayed response, high cost, and difficulty in achieving high-frequency monitoring. Electrochemical sensor technology, with its advantages of high sensitivity, rapid response, miniaturization and low cost, has shown great potential in the field of medical testing. It is particularly suitable for the ultra-sensitive, real-time and bedside dynamic monitoring of key markers of HCC such as AFP and PIVKA-II, and is expected to provide more precise efficacy feedback and early warning for complex combination therapy. This study adopted a retrospective cohort design, including 102 patients with advanced HCC who received quadruple or triple therapy. The objective response rate (ORR) was evaluated by the mRECIST criteria, and progression-free survival (PFS), overall survival (OS), and adverse reactions were recorded. The results showed that the ORR (52.1% vs. 31.5%), mPFS (10.8 vs. 7.2 months), and mOS (23.5 vs. 18.1 months) in the quadruple group were significantly better than those in the triple group, and the conversion resection rate was higher (25.0% vs. 7.4%). Although the incidence of postoperative pain and nausea and vomiting is slightly higher, the overall safety is controllable. It is worth emphasizing that the results of this study highlight the urgent need for high-frequency and high-sensitivity biomarker monitoring systems, and electrochemical sensor technology can precisely provide an ideal solution for such high-intensity combined treatments. By achieving real-time dynamic tracking of molecules related to tumor burden and drug toxicity, this technology is expected to further optimize the effective regimens verified in this study.

本研究旨在比较TACE- haic联合靶向免疫治疗（四联疗法）与TACE联合靶向免疫治疗（三联疗法）治疗晚期肝细胞癌（HCC）的临床疗效和安全性。虽然目前肝癌的全身与局部联合治疗策略已取得进展，但对疗效的动态、精准评估仍高度依赖于传统的影像学和间歇性血清标志物检测，存在反应滞后、成本高、难以实现高频监测等局限性。电化学传感器技术以其高灵敏度、快速响应、小型化和低成本等优点，在医学检测领域显示出巨大的潜力。尤其适用于AFP、PIVKA-II等HCC关键标志物的超灵敏、实时、床边动态监测，有望为复杂联合治疗提供更精准的疗效反馈和预警。本研究采用回顾性队列设计，纳入102例接受四联或三联治疗的晚期HCC患者。根据mRECIST标准评估客观缓解率（ORR），记录无进展生存期（PFS）、总生存期（OS）和不良反应。结果显示，四联体组的ORR （52.1% vs. 31.5%）、mPFS （10.8 vs. 7.2个月）、mOS （23.5 vs. 18.1个月）均显著优于三联体组，且转阴切除率（25.0% vs. 7.4%）较高。虽然术后疼痛和恶心呕吐的发生率略高，但总体安全性可控。值得强调的是，本研究结果突出了对高频、高灵敏度生物标志物监测系统的迫切需求，电化学传感器技术可以精确地为这种高强度联合治疗提供理想的解决方案。通过实现对肿瘤负荷和药物毒性相关分子的实时动态跟踪，该技术有望进一步优化本研究验证的有效方案。

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引用次数: 0

Characterization of polysaccharides from oenological byproducts by a novel multiple reaction monitoring mass spectrometry method 用一种新的多反应监测质谱法对酿酒副产物中多糖进行表征

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-12 DOI: 10.1016/j.microc.2026.117309

Montserrat Dueñas, Diana M. Bosch-Crespo, Natalia Rojo-Nieto, Iván Puerta-García, Elvira Manjón, María Teresa Escribano-Bailón, Ignacio García-Estévez

A robust analytical method based on derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) and subsequent analysis by HPLC-MS/MS-multiple reaction monitoring (MRM) has been developed and validated for the quantification of monosaccharides in different oenological byproducts (commercial mannoproteins, wine lees and grape pomace extracts). This methodology overcomes most of the limitations associated with the conventional techniques most commonly used in oenology. Method validation demonstrated good precision and accuracy, with low limits of detection (LOD) and quantification (LOQ). Mannose and glucose were identified as the main monosaccharides in commercial mannoprotein extracts and wine lees extracts (53–97%), while galacturonic acid, arabinose, and galactose (∼34% each) dominated in white grape pomace polysaccharide extract, indicating the presence of homogalacturonans (HG) and polysaccharides rich in arabinose and galactose (PRAGs). These results highlight the important structural and compositional variability among oenological extracts, whether commercially available or obtained in the laboratory, reinforcing the need for a robust methodology to determine their composition. Hence, the validated method presented here can be a valuable tool to enhance the understanding of such extracts by providing key chemical information that is crucial for assessing their applicability and technological properties.

建立了一种基于1-苯基-3-甲基-5-吡唑酮（PMP）衍生化和随后的HPLC-MS/ ms -多重反应监测（MRM）分析的可靠分析方法，并验证了该方法用于定量不同酿酒副产物（商业甘露蛋白、酒渣和葡萄渣提取物）中的单糖。这种方法克服了与酿酒学中最常用的传统技术相关的大多数限制。方法验证精密度和准确度高，检出限和定量限低。在商业甘露蛋白提取物和酒渣提取物中，甘露糖和葡萄糖是主要的单糖（53-97%），而在白葡萄渣多糖提取物中，半乳糖醛酸、阿拉伯糖和半乳糖（各占~ 34%）占主导地位，表明存在高半乳糖醛酸（HG）和富含阿拉伯糖和半乳糖的多糖（PRAGs）。这些结果突出了酿酒提取物之间的重要结构和组成差异，无论是在市售还是在实验室获得，都强调了确定其组成的可靠方法的必要性。因此，本文提出的经过验证的方法可以成为一个有价值的工具，通过提供对评估其适用性和技术特性至关重要的关键化学信息来增强对此类提取物的理解。

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引用次数: 0

Three-dimensional porous ZnCo-LDH/ZnCo-NH hybrid structure for electro-enhanced thin film microextraction of some acidic red dyes from various cosmetic and food samples 三维多孔ZnCo-LDH/ZnCo-NH杂化结构用于电增强薄膜微萃取化妆品和食品样品中的酸性红色染料

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-13 DOI: 10.1016/j.microc.2026.117371

Maedeh Saadat, Yadollah Yamini

A three-dimensional porous nanoheterostructures of ZnCo-LDH/ZnCo-NH were synthesized on a nickel foam substrate. These structures were employed as efficient adsorbents for electro-enhanced thin film microextraction of red dyes from various cosmetic and food samples. The method, followed by high-performance liquid chromatography coupled with a UV detector. The prepared LDH was characterized using various techniques. In addition, effective parameters such as elution solvent (ethanol +0.075% NaNO₃), desorption time (5 min), adsorption time (20 min), sorbent length (2 cm), desorption volume (600 μL), breakthrough volume (10 mL), pH (9), salt addition (0%), adsorption potential (1 V), and desorption potential (1.5 V) were optimized. Under the optimum conditions, the method demonstrated a great linear dynamic range of 0.05–500 μg L⁻¹, with an acceptable limit of detection (LOD) of 0.02 μg L⁻¹. The intra-day and interday relative standard deviations (RSDs) were in the ranges of 2.4%–4.8% and 3.9%–6.1%, respectively. Additionally, R² values were measured to be 0.9948, 0.9970, 0.9901, 0.9976, and 0.9931 for Amaranth, Ponceau 4R, Allura Red, Carmosine, and Erythrosine, respectively. Finally, this method was employed for the extraction and preconcentration of red dyes from various cosmetic and food samples with suitable and satisfactory outcomes.

在泡沫镍基体上合成了ZnCo-LDH/ZnCo-NH的三维多孔纳米异质结构。这些结构被用作电增强薄膜微萃取各种化妆品和食品样品中的红色染料的有效吸附剂。该方法，其次是高效液相色谱耦合紫外检测器。用各种技术对所制备的LDH进行了表征。此外，优化了洗脱溶剂（乙醇+0.075% NaNO3）、解吸时间（5 min）、吸附时间（20 min）、吸附剂长度（2 cm）、解吸体积（600 μL）、突破体积（10 mL）、pH(9)、盐添加量（0%）、吸附电位（1 V）、解吸电位（1.5 V）等有效参数。在最佳条件下，该方法线性动态范围为0.05 ~ 500 μg L−1，可接受检出限为0.02 μg L−1。日内、日间相对标准偏差（rsd）分别为2.4% ~ 4.8%和3.9% ~ 6.1%。苋菜红、Ponceau 4R、紫花红、Carmosine和erythrosin的R2分别为0.9948、0.9970、0.9901、0.9976和0.9931。最后，将该方法应用于各种化妆品和食品样品中红色染料的提取和富集，取得了满意的结果。

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引用次数: 0

Cyanoethyl cellulose–based molecularly imprinted polymer for selective S-suprofen recognition 基于氰乙基纤维素的分子印迹聚合物选择性S-suprofen识别

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-02 DOI: 10.1016/j.microc.2026.117153

Munirah S.O. Alhar , Odeh A.O. Alshammari , Ahmed A. AlOtaibi , Aljazi Abdullah AlRashidi , Ahlam F. Alshammari , Elham A. Alzahrani , Nadia H. Elsayed , Khadra B. Alomari , M. Monier

Suprofen (SF) is a chiral nonsteroidal anti-inflammatory drug whose therapeutic activity resides almost exclusively in the S-enantiomer, necessitating efficient enantioselective separation for safe clinical use. Here we report a novel molecularly imprinted polymer (MIP) based on cyanoethyl cellulose (CNCE) chemically modified with 1-amino-1H-pyrrole-2,5-dione (AMA). AMA introduces cationic hydrazidine groups for electrostatic and hydrogen-bonding interactions with anionic S-SF and simultaneously grafts maleimide moieties onto the polymer backbone. Follow-up thiol–maleimide click crosslinking with ethane-1,2-dithiol (ET) sequesters stereospecific recognition cavities to enhance structural fidelity and selectivity. The as-prepared S-SF-imprinted microparticles (S-SF-P) possess high surface area, well-defined porosity, and intense surface roughness, as confirmed by BET and SEM characterizations. Batch adsorption and column experiments showed high chiral selectivity, with S-SF adsorption capacity of 522 ± 3 mg g⁻¹, a selectivity coefficient of ∼13 over R-SF, and eluates reaching 96.9% enantiomeric excess, reflecting the strong performance of the post-imprinted material within its class. Kinetic and thermodynamic examination of the click crosslinking reveals a spontaneous, exothermic reaction that allows for stable network development. This stable, low-cost, renewable cellulose-based MIP with precise post-polymerization crosslinking and dual-functionalization is a platform for high-fidelity drug and other chiral target enantioseparation.

苏洛芬（SF）是一种手性非甾体抗炎药，其治疗活性几乎完全存在于s对映体中，需要有效的对映体选择性分离才能安全临床使用。本文报道了一种以氰乙基纤维素（CNCE）为基础，经1-氨基- 1h -吡咯-2,5-二酮（AMA）化学修饰的新型分子印迹聚合物（MIP）。AMA引入了阳离子肼基团，与阴离子S-SF进行静电和氢键相互作用，同时将马来酰亚胺部分接枝到聚合物主链上。后续巯基马来酰亚胺与乙烷-1,2-二硫醇（ET）螯合物进行立体特异性识别空腔的点击交联，以提高结构保真度和选择性。制备的s- sf印迹微粒（S-SF-P）具有高表面积，明确的孔隙率和强烈的表面粗糙度，如BET和SEM表征所证实的那样。批吸附和柱实验表明，S-SF的吸附量为522±3 mg g - 1，对R-SF的选择性系数为~ 13，洗脱物对映体过量达到96.9%，反映了该后印迹材料在同类材料中的强大性能。动力学和热力学检查的点击交联揭示了一个自发的，放热反应，允许稳定的网络发展。这种稳定、低成本、可再生的纤维素基MIP具有精确的聚合后交联和双功能化，是高保真药物和其他手性靶对映体分离的平台。

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引用次数: 0

Computation-guided design of a Laccase-COOH-SWCNT/SPCE electrochemical biosensor for selective tyramine detection 用于酪胺选择性检测的laccase - cooh - swcnts /SPCE电化学生物传感器的计算指导设计

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-12 DOI: 10.1016/j.microc.2026.117343

Nurul Hana Mas'od , Mohammad Nazri Abdul Bahari , Syaza Azhari , Mohamad Arif Mohamad Jamali , Nadrahtul Huda Misral , Amir Syahir Amir Hamzah

Tyramine (TYM) is a key spoilage biomarker in meat products, creating a need for rapid and selective on-site detection technologies. This study presents a computation-guided strategy integrating molecular docking, molecular dynamics (MD), and molecular mechanics Poisson–Boltzmann surface area (MM/PBSA) free-energy analysis with experimental biosensor fabrication to rationalize and enhance TYM recognition by laccase (LAC). Docking and MD simulations revealed that TYM binds within a hydrophobic pocket near the T1 copper site, anchored by a hydrogen bond with Ala80 and stabilized by Phe344, Pro346, Leu112, and Leu459. The LAC–TYM complex remained structurally stable over 100 ns, and MM/PBSA yielded a favorable binding free energy (ΔG_bind ≈ −22.6 kcal/mol), predominantly driven by van der Waals interactions. Guided by these atomistic insights, a LAC–COOH-SWCNT/SPCE biosensor was constructed and characterized by field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM), confirming uniform enzyme immobilization. Differential pulse voltammetry (DPV) exhibited a linear range of 2.58 × 10⁻⁵ to 1.92 × 10⁻⁴ M with a limit of detection (LOD) of 7.75 × 10⁻⁶ M, a limit of quantification (LOQ) of 2.58 × 10⁻⁶ M, a sensitivity of 0.0136 μA mM⁻¹ and exhibited a high correlation coefficient (R² = 0.984). The sensor demonstrated strong selectivity, minimal interference from structurally unrelated biogenic amines, and good recoveries (78.2–105.8%) in spiked chicken meat samples. This work establishes a unified computation-to-experiment framework for biosensor engineering and highlights the potential of atomistic modelling to guide enzyme–nanomaterial interface design for food safety applications.

酪胺（TYM）是肉制品中重要的腐败生物标志物，因此需要快速、选择性的现场检测技术。本研究提出了一种结合分子对接、分子动力学（MD）、分子力学泊松-玻尔兹曼表面积（MM/PBSA）自由能分析和实验生物传感器制作的计算导向策略，以优化和增强漆酶（LAC）对TYM的识别。对接和MD模拟表明，TYM结合在T1铜位点附近的疏水口袋内，由与Ala80的氢键锚定，并由Phe344、Pro346、Leu112和Leu459稳定。LAC-TYM配合物在100 ns内保持结构稳定，MM/PBSA产生良好的结合自由能（ΔG_bind≈- 22.6 kcal/mol），主要由范德华相互作用驱动。在这些原子观的指导下，构建了LAC-COOH-SWCNT /SPCE生物传感器，并通过场发射扫描电镜（FESEM）和原子力显微镜（AFM）对其进行了表征，证实了酶的均匀固定。差分脉冲伏安法的线性范围为2.58 × 10−5 ~ 1.92 × 10−4 M，检出限为7.75 × 10−6 M，定量限为2.58 × 10−6 M，灵敏度为0.0136 μA mM−1，相关系数高（R2 = 0.984）。该传感器具有很强的选择性，对结构无关的生物胺干扰最小，在加标鸡肉样品中具有良好的回收率（78.2-105.8%）。这项工作为生物传感器工程建立了一个统一的从计算到实验的框架，并强调了原子建模在指导食品安全应用中酶-纳米材料界面设计方面的潜力。

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引用次数: 0

Ratiometric electrochemical quantitation of methyl parathion based on NiHCF/PDDA@rGO nanocomposites 基于NiHCF/PDDA@rGO纳米复合材料的甲基对硫磷比例电化学定量

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.microc.2026.117344

Xiaolin Jia , Miaomiao Li , Zhiguang Liu , Yujie Han , Hua-Zhong Yu , Lifang Fan , Yujing Guo

This work presents an on-off ratiometric electrochemical quantitation study of methyl parathion (MP), one of the most popularly used organophosphorus pesticides, based on electrode modification with a newly developed nanocomposite, i.e., reduced graphene oxide (rGO) functionalized with poly(diallyldimethylammonium chloride) (PDDA) and nickel hexacyanoferrate (NiHCF). It was shown that MP strongly adsorbs on the modified electrodes and produces a distinct reduction signal (-NO₂), which increases proportionally with its concentration; meanwhile, the intrinsic reduction signal (Fe(III)/(II)) from the nanocomposite (NiHCF/PDDA@rGO) decreases due to the inhibited electron-transfer process upon MP binding. Indeed, plotting the ratiometric signal (the ratio of decreased Fe(III)/(II) current and the increased MP current) as a function of the analyte (MP) concentration produces a linear calibration curve in the range of 0.05 μM to 10 μM (R² = 0.9987), with a limit of detection (LOD) of 16 nM. The method was further validated in real water samples, showing recoveries of 96.2–102% with relative standard deviations below 3.3%, which are comparable with standard HPLC results.

本文介绍了一种基于电极修饰的新型纳米复合材料，即由聚二烯基二甲基氯化铵（PDDA）和六氰铁酸镍（NiHCF）功能化的还原氧化石墨烯（rGO），对最常用的有机磷农药之一甲基对硫磷（MP）进行了开关比电化学定量研究。结果表明，MP在改性电极上具有较强的吸附作用，并产生明显的还原信号（-NO2），其还原信号随其浓度成比例增加；同时，纳米复合材料（NiHCF/PDDA@rGO）的本特征还原信号(Fe（III)/(II)）由于MP结合时电子转移过程受到抑制而降低。事实上，将比值信号(Fe（III)/(II)电流下降与MP电流增加的比值）与分析物（MP）浓度的关系绘制为0.05 μM ~ 10 μM的线性校准曲线（R2 = 0.9987），检出限（LOD）为16 nM。在实际水样中进一步验证，回收率为96.2 ~ 102%，相对标准偏差小于3.3%，与标准HPLC结果相当。

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引用次数: 0

Highly efficient V2O5@In2O3 nanoflowers for outstanding NO2 gas sensing proficiencies 高效V2O5@In2O3纳米花出色的NO2气体传感熟练

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-13 DOI: 10.1016/j.microc.2026.117359

Shahid Hussain , Zhenyu Miao , Yuanyuan Han , Adil Shafi Ganie , Abdul Jalil , Mohammed Mujahid Alam , Abdullah G. Al-Sehemi , Rajesh Kumar Manavalan , Xiangzhao Zhang , Guanjun Qiao

The global issue of nitrogen dioxide pollution has become a significant environmental concern due to its significant impact on the environment. In this paper, V₂O₅ nanoflowers were successfully prepared by a facile hydrothermal method using ammonium metavanadate as the precursor, representing the fabrication of V₂O₅ with a morphology assembled from numerous fine nanorods into nanoflower-like structures. To enhance the gas-sensitive properties of V₂O₅ nanomaterials, V₂O₅/In₂O₃ nanocomposites were successfully synthesized by a two-step hydrothermal method subsequently. This study presents the first instance in the field of gas-sensing research where the gas sensing performance of V₂O₅ has been enhanced by constructing an n-n heterojunction through composite formation with In₂O₃. In order to further verify the synergistic effect of the nanocomposites on the gas-sensitized performance, the effects of different composite ratios of V₂O₅/In₂O₃ nanocomposites on the gas-sensitized performance were investigated. The results showed that the gas-sensitized performance of V₂O₅/In₂O₃ nanocomposites with a ratio of 3:1 was optimal, and the response of the material to 100 ppm NO₂ at an operating temperature of 200 °C reached 20.4. The material exhibits high response, good selectivity, repeatability and low detection limit, and is moisture resistant. The excellent performance was attributed to the preparation of nanocomposites providing more adsorption sites and the synergistic effect created by the construction of n-n heterojunctions.

二氧化氮污染因其对环境的重大影响而成为一个全球性的环境问题。在本文中，使用超氰酸铵作为前驱体，通过简便的水热方法成功制备了V2O5纳米花，代表了由许多精细纳米棒组装成纳米花状结构的V₂O₅的制造。为了提高V2O5纳米材料的气敏性能，采用两步水热法制备了V2O5/In2O3纳米复合材料。该研究首次在气敏研究领域通过与In2O3复合形成n-n异质结来增强V2O5的气敏性能。为了进一步验证纳米复合材料对气敏性能的协同作用，研究了V2O5/In2O3纳米复合材料不同配比对气敏性能的影响。结果表明：V2O5/In2O3纳米复合材料的气敏性能为3:1时最佳，在200℃的工作温度下，对100 ppm NO2的响应达到20.4；该材料具有响应性高、选择性好、重复性好、检出限低、耐湿等特点。这种优异的性能归功于纳米复合材料的制备提供了更多的吸附位点，以及n-n异质结的构建所产生的协同效应。

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引用次数: 0

Electrochemical sensors used for identify autophagy-related hub genes and their correlation with immune infiltration in nasopharyngeal carcinoma: A bioinformatics analysis combined with experimental validation 电化学传感器用于鉴定鼻咽癌中自噬相关中心基因及其与免疫浸润的相关性：生物信息学分析结合实验验证

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.microc.2026.117353

Bo Zheng, Haiyong Jin, Xiaoqiong Wang, Xuejun Liu

Electrochemical sensor technology converts biological recognition events into quantifiable electrical signals, enabling direct and efficient detection of trace proteins, nucleic acids, and exosomes in complex body fluids. It shows broad application prospects in cancer early screening, therapeutic efficacy monitoring, and individualized treatment decision-making. The research obtained NPC transcriptome data from public databases (such as TCGA, GEO), screened differentially expressed autophagy-related genes using bioinformatics methods, and conducted functional enrichment, protein interaction network construction, and immune infiltration correlation analysis. The study did not conduct experimental verification; all results were derived from the re-examination and integrated analysis of existing data. Some autophagy genes were closely related to the patient's prognosis, suggesting their potential clinical value. The study revealed the important role of autophagy regulation in the shaping of the NPC immune microenvironment. The high-specificity electrochemical detection platform based on these targets is expected to achieve non-invasive and dynamic monitoring of the autophagy status and immune response of NPC patients, thereby promoting the implementation of precise diagnosis and treatment strategies.

电化学传感器技术将生物识别事件转化为可量化的电信号，能够直接有效地检测复杂体液中的微量蛋白质、核酸和外泌体。在肿瘤早期筛查、疗效监测、个体化治疗决策等方面具有广阔的应用前景。本研究从公共数据库（如TCGA、GEO）获取NPC转录组数据，利用生物信息学方法筛选差异表达的自噬相关基因，并进行功能富集、蛋白相互作用网络构建、免疫浸润相关性分析。本研究未进行实验验证；所有结果均来自对现有资料的重新检查和综合分析。一些自噬基因与患者预后密切相关，提示其潜在的临床价值。本研究揭示了自噬调控在鼻咽癌免疫微环境形成中的重要作用。基于这些靶点的高特异性电化学检测平台有望实现对鼻咽癌患者自噬状态和免疫反应的无创动态监测，从而促进精准诊疗策略的实施。

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引用次数: 0