Microchemical Journal最新文献

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Silver nanoparticles doped three-dimensional hydrogel for electrochemical simultaneous sensing of heavy metal ions

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112304

Linghui Tang , Yue Wang , Wenwen Yu , Yan Dong , Yasushi Hasebe

Heavy metal ions have significant risks to both environment and human health, and their detrimental effects are irreversible. Therefore, a sensor capable of simultaneous detecting heavy metal ions with high accuracy presents a significant challenge. In this work, a conductive hydrogel was synthesized using 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), methacrylamide (MAM), and hydroxyethyl methacrylate (HEMA) as monomers, N, N’-methylenebis(acrylamide) as the crosslinker, through physically doping with silver nanoparticles. The prepared hydrogel has the characteristics of good conductivity, excellent swelling ability and high adsorption capacity for enrichment of heavy metal ions (HIMs). The three-dimensional network hydrogel was directly used for simultaneous detection of HIMs in multiplex system, with superior separation ability, good linear ranges, excellent reproducibility and good long-time stability. Additionally, this sensor can accurately detect Cu²⁺ in river sample, demonstrating high precision and applicability. The fabrication of the hydrogel electrochemical sensor is simple and mild, allowing for the electrochemical capture of various HIMs and holds practical application prospect.

{"title":"Silver nanoparticles doped three-dimensional hydrogel for electrochemical simultaneous sensing of heavy metal ions","authors":"Linghui Tang , Yue Wang , Wenwen Yu , Yan Dong , Yasushi Hasebe","doi":"10.1016/j.microc.2024.112304","DOIUrl":"10.1016/j.microc.2024.112304","url":null,"abstract":"<div><div>Heavy metal ions have significant risks to both environment and human health, and their detrimental effects are irreversible. Therefore, a sensor capable of simultaneous detecting heavy metal ions with high accuracy presents a significant challenge. In this work, a conductive hydrogel was synthesized using 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), methacrylamide (MAM), and hydroxyethyl methacrylate (HEMA) as monomers, N, N’-methylenebis(acrylamide) as the crosslinker, through physically doping with silver nanoparticles. The prepared hydrogel has the characteristics of good conductivity, excellent swelling ability and high adsorption capacity for enrichment of heavy metal ions (HIMs). The three-dimensional network hydrogel was directly used for simultaneous detection of HIMs in multiplex system, with superior separation ability, good linear ranges, excellent reproducibility and good long-time stability. Additionally, this sensor can accurately detect Cu<sup>2+</sup> in river sample, demonstrating high precision and applicability. The fabrication of the hydrogel electrochemical sensor is simple and mild, allowing for the electrochemical capture of various HIMs and holds practical application prospect.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112304"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-enzyme simulation activity of MXene-based metal composites and the application for detection and degradation of phenolic pollutants

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112350

He Huang, Qinqin Yang, Yu He, Gongwu Song

Phenolic pollutants are commonly employed in industries such as petrochemicals, dyeing, textiles, and pesticides, which cause great harm to our living environment and food safety. In this paper, the MXenes-based metal composites (M/MXenes, M = Cu, Co, Ni, Zn, Fe, Mn) with multi-enzyme mimic activity were prepared by a facile molten salt method. The M/MXenes have mimic catalytic activity of peroxidase (POD), ascorbic acid oxidase (AAO), catalase (CAT), superoxide dismutase (SOD) and laccase. The catalytic properties of M/MXenes were analyzed and discussed in detail. In addition, M/MXenes still have excellent catalytic performance and stability in harsh pH and high temperature environments, making them very suitable for practical applications. Due to the excellent laccase mimic activity of M/MXenes, a simple colorimetric sensor was constructed for the detection and degradation of phenolic pollutants. Because obvious color changes can be observed during the catalytic process of phenolic pollutants by M/MXenes, M/MXenes were fixed into sodium alginate (SA) to form a simple hydrogel-based sensing platform for 2, 4-chlorophenol, methoxyphenol, 4-bromophenol, 3-bromo-4-methoxyphenol, 3-bromo-4-methylphenol, hydroquinone and 1,8-naphthol. By introducing different concentrations of phenolic compounds, a series of color changes on the gel carrier can be observed by naked eyes. Then, the red (R), green (G) and blue (B) values matching with the color change of the hydrogel were read out by the color recognition application in the smartphone, and the RGB value was converted into the concentration of phenolic compounds, which offered a convenient and reliable method for the rapid detection of phenolic compounds. Thus, the strategy offers the potential in rational design of nanozymes with the advantages of multi-enzyme mimic activity, simple preparation, low cost and good stability.

{"title":"Multi-enzyme simulation activity of MXene-based metal composites and the application for detection and degradation of phenolic pollutants","authors":"He Huang, Qinqin Yang, Yu He, Gongwu Song","doi":"10.1016/j.microc.2024.112350","DOIUrl":"10.1016/j.microc.2024.112350","url":null,"abstract":"<div><div>Phenolic pollutants are commonly employed in industries such as petrochemicals, dyeing, textiles, and pesticides, which cause great harm to our living environment and food safety. In this paper, the MXenes-based metal composites (M/MXenes, M = Cu, Co, Ni, Zn, Fe, Mn) with multi-enzyme mimic activity were prepared by a facile molten salt method. The M/MXenes have mimic catalytic activity of peroxidase (POD), ascorbic acid oxidase (AAO), catalase (CAT), superoxide dismutase (SOD) and laccase. The catalytic properties of M/MXenes were analyzed and discussed in detail. In addition, M/MXenes still have excellent catalytic performance and stability in harsh pH and high temperature environments, making them very suitable for practical applications. Due to the excellent laccase mimic activity of M/MXenes, a simple colorimetric sensor was constructed for the detection and degradation of phenolic pollutants. Because obvious color changes can be observed during the catalytic process of phenolic pollutants by M/MXenes, M/MXenes were fixed into sodium alginate (SA) to form a simple hydrogel-based sensing platform for 2, 4-chlorophenol, methoxyphenol, 4-bromophenol, 3-bromo-4-methoxyphenol, 3-bromo-4-methylphenol, hydroquinone and 1,8-naphthol. By introducing different concentrations of phenolic compounds, a series of color changes on the gel carrier can be observed by naked eyes. Then, the red (R), green (G) and blue (B) values matching with the color change of the hydrogel were read out by the color recognition application in the smartphone, and the RGB value was converted into the concentration of phenolic compounds, which offered a convenient and reliable method for the rapid detection of phenolic compounds. Thus, the strategy offers the potential in rational design of nanozymes with the advantages of multi-enzyme mimic activity, simple preparation, low cost and good stability.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112350"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lightweight spatial pyramid pooling convolutional neural network assisted hyperspectral imaging for Hangbaiju origin identification

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112352

Ming-Yue Dong , Wan-Jun Long , Hai-Long Wu , Tong Wang , Hai-Yan Fu , Kun Huang , Hang Ren , Ru-Qin Yu

Hangbaiju is a popular food supplement in the form of tea whose quality is influenced by the geographical origin. To control food quality, this work proposed a novel method, hyperspectral spatial pyramid pooling convolutional neural network (HSPPnet) assisted hyperspectral imaging (HSI), to identify the origin of Hangbaiju. HSPPnet utilizes both spatial and spectral information from hyperspectral images effectively and tolerates images of various sizes. This study initially evaluated the performance of four classical classification algorithms (k-NN, RF, XGBoost and PLS-DA) using hyperspectral average spectra, uncovering limitations in distinguishing samples with severe spectral overlap. Additionally, three representative deep learning models (VGG16, ResNet18, and DenseNet121) were used to analyze hyperspectral images compressed into three channels, resulting in overfitting due to the loss of detail information. Finally, HSPPnet and three improved deep learning models (VGG16-25, ResNet18-25, and DenseNet121-25) were used for Hangbaiju origin identification. HSPPnet achieved 100.0% accuracy for both the test set and prediction set, and three improved deep learning models also had been significantly improved. Through comparison, it was found that HSPPnet is an efficient and lightweight model, boasting the benefits of being faster, smaller, and more power-efficient. It was more suitable for deployment on HSI devices to realize large-scale inline detection. The interpretability of HSPPnet was studied to explore the pixel features of Hangbaiju hyperspectral images that were helpful for identification. Additionally, the proposed method exhibited excellent greenness and whiteness properties. The results showed that the proposed method was a non-destructive, efficient, and green method for Hangbaiju origin identification.

{"title":"Lightweight spatial pyramid pooling convolutional neural network assisted hyperspectral imaging for Hangbaiju origin identification","authors":"Ming-Yue Dong , Wan-Jun Long , Hai-Long Wu , Tong Wang , Hai-Yan Fu , Kun Huang , Hang Ren , Ru-Qin Yu","doi":"10.1016/j.microc.2024.112352","DOIUrl":"10.1016/j.microc.2024.112352","url":null,"abstract":"<div><div>Hangbaiju is a popular food supplement in the form of tea whose quality is influenced by the geographical origin. To control food quality, this work proposed a novel method, hyperspectral spatial pyramid pooling convolutional neural network (HSPPnet) assisted hyperspectral imaging (HSI), to identify the origin of Hangbaiju. HSPPnet utilizes both spatial and spectral information from hyperspectral images effectively and tolerates images of various sizes. This study initially evaluated the performance of four classical classification algorithms (<em>k</em>-NN, RF, XGBoost and PLS-DA) using hyperspectral average spectra, uncovering limitations in distinguishing samples with severe spectral overlap. Additionally, three representative deep learning models (VGG16, ResNet18, and DenseNet121) were used to analyze hyperspectral images compressed into three channels, resulting in overfitting due to the loss of detail information. Finally, HSPPnet and three improved deep learning models (VGG16-25, ResNet18-25, and DenseNet121-25) were used for Hangbaiju origin identification. HSPPnet achieved 100.0% accuracy for both the test set and prediction set, and three improved deep learning models also had been significantly improved. Through comparison, it was found that HSPPnet is an efficient and lightweight model, boasting the benefits of being faster, smaller, and more power-efficient. It was more suitable for deployment on HSI devices to realize large-scale inline detection. The interpretability of HSPPnet was studied to explore the pixel features of Hangbaiju hyperspectral images that were helpful for identification. Additionally, the proposed method exhibited excellent greenness and whiteness properties. The results showed that the proposed method was a non-destructive, efficient, and green method for Hangbaiju origin identification.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112352"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free photoelectrochemical aptasensor utilizing Ag2S quantum dot-sensitized In2O3/BiOBr heterojunction for β-lactoglobulin detection in milk samples

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112564

Meixin Li , Pengfei Yu , Shanshan Li , Mati Ullah Khan , Shuang Liu , Jun Yang , Jiaqi Cui , Ling Kong , Zhiwei Chen

Cow’s milk allergy is a prevalent food allergy, with β-lactoglobulin (β-Lg) identified as one of the major allergenic proteins in cow’s milk. The accurate detection of β-Lg is crucial for reducing the risk of allergic reactions. In this study, a novel label-free photoelectrochemical (PEC) aptasensor was developed for the detection of β-Lg in milk, utilizing an In₂O₃/BiOBr heterojunction composite material. Sensitization of the heterojunction with Ag₂S quantum dots (QDs) enhanced the photosensitivity, leading to a significant increase in photocurrent response. The In₂O₃/BiOBr heterojunction improved solar energy utilization and enhanced light absorption across the visible spectrum. The photocurrent signal intensity decreased proportionally with increasing β-Lg concentration due to the specific binding with the aptamer. Under optimized experimental conditions, the PEC aptasensor exhibited a wide linear detection range from 0.001 ng/mL to 100 ng/mL and achieved a detection limit of 2.31 pg/mL. The sensor proved excellent selectivity, stability, reproducibility, and a broad linear range, offering a promising method for β-Lg detection in real milk samples.

{"title":"A label-free photoelectrochemical aptasensor utilizing Ag2S quantum dot-sensitized In2O3/BiOBr heterojunction for β-lactoglobulin detection in milk samples","authors":"Meixin Li , Pengfei Yu , Shanshan Li , Mati Ullah Khan , Shuang Liu , Jun Yang , Jiaqi Cui , Ling Kong , Zhiwei Chen","doi":"10.1016/j.microc.2024.112564","DOIUrl":"10.1016/j.microc.2024.112564","url":null,"abstract":"<div><div>Cow’s milk allergy is a prevalent food allergy, with β-lactoglobulin (β-Lg) identified as one of the major allergenic proteins in cow’s milk. The accurate detection of β-Lg is crucial for reducing the risk of allergic reactions. In this study, a novel label-free photoelectrochemical (PEC) aptasensor was developed for the detection of β-Lg in milk, utilizing an In<sub>2</sub>O<sub>3</sub>/BiOBr heterojunction composite material. Sensitization of the heterojunction with Ag<sub>2</sub>S quantum dots (QDs) enhanced the photosensitivity, leading to a significant increase in photocurrent response. The In<sub>2</sub>O<sub>3</sub>/BiOBr heterojunction improved solar energy utilization and enhanced light absorption across the visible spectrum. The photocurrent signal intensity decreased proportionally with increasing β-Lg concentration due to the specific binding with the aptamer. Under optimized experimental conditions, the PEC aptasensor exhibited a wide linear detection range from 0.001 ng/mL to 100 ng/mL and achieved a detection limit of 2.31 pg/mL. The sensor proved excellent selectivity, stability, reproducibility, and a broad linear range, offering a promising method for β-Lg detection in real milk samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112564"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scintillating iron imprinted polymers (Sc-Fe-IIP): Novel material for 55Fe selective recognition

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112268

I. Giménez , H. Bagán , A. Tarancón

In the coming years, numerous nuclear power plants are expected to reach the end of their operational lifespans, leading to a substantial increase in the demand for analyses during the decommissioning process. Of particular concern is ⁵⁵Fe, a fission product originating from neutron activation of stable iron. The conventional methods for determining ⁵⁵Fe using liquid scintillation are known for being time-consuming, complex, and involving multiple steps to eliminate interferences. In this work, the first scintillating imprinted polymer for ⁵⁵Fe has been developed (Sc-Fe-IIP). Several tests have been conducted to define the polymerization procedure, the components of the polymer, and their proportions, making it possible to combine selective retention and scintillation capabilities for ⁵⁵Fe determination in one material. The optimum Sc-Fe-IIP is composed of styrene, divinylbenzene, and vinyl phosphonic acid, together with a complex of Fe with acrylic acid and scintillation fluorescence solutes. The iron capacity is up to 17 mg per gram of Sc-Fe-IIP, and the selectivity is 95 %, with reduced retention of copper (4 %) and no retention of nickel or cobalt. Regarding scintillation capabilities, the Sc-Fe-IIP showed a detection efficiency of 4.90 %.

{"title":"Scintillating iron imprinted polymers (Sc-Fe-IIP): Novel material for 55Fe selective recognition","authors":"I. Giménez , H. Bagán , A. Tarancón","doi":"10.1016/j.microc.2024.112268","DOIUrl":"10.1016/j.microc.2024.112268","url":null,"abstract":"<div><div>In the coming years, numerous nuclear power plants are expected to reach the end of their operational lifespans, leading to a substantial increase in the demand for analyses during the decommissioning process. Of particular concern is <sup>55</sup>Fe, a fission product originating from neutron activation of stable iron. The conventional methods for determining <sup>55</sup>Fe using liquid scintillation are known for being time-consuming, complex, and involving multiple steps to eliminate interferences. In this work, the first scintillating imprinted polymer for <sup>55</sup>Fe has been developed (Sc-Fe-IIP). Several tests have been conducted to define the polymerization procedure, the components of the polymer, and their proportions, making it possible to combine selective retention and scintillation capabilities for <sup>55</sup>Fe determination in one material. The optimum Sc-Fe-IIP is composed of styrene, divinylbenzene, and vinyl phosphonic acid, together with a complex of Fe with acrylic acid and scintillation fluorescence solutes. The iron capacity is up to 17 mg per gram of Sc-Fe-IIP, and the selectivity is 95 %, with reduced retention of copper (4 %) and no retention of nickel or cobalt. Regarding scintillation capabilities, the Sc-Fe-IIP showed a detection efficiency of 4.90 %.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112268"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porous Pt nanodendrites with exceptionally high oxidase-like mimic catalytic efficiency for ratiometric fluorescence sensing of ascorbic acid and acid phosphatase

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112277

Yajun Zhong , Tiantian Xie , Jingyun Zhang , Wenyuan Liu , Qiuxiang Wang , Zhenjing Zhuang

In this study, three porous platinum nanodendrites (Pt NDs) with exceptionally high catalytic efficiencies were fabricated using a carbon-dot (CD)-mediated route. This study demonstrated that the as-prepared porous Pt NDs, such as the integration of a large number of small Pt nanozymes with excellent catalytic activity, exhibited larger sizes and higher oxidase-like catalytic efficiency. The highest K_cat value of the obtained porous Pt NDs was 8.61 × 10⁴ s⁻¹, which was 20-fold greater than that of HRP (K_cat = 4.3 × 10³ s⁻¹). A mechanistic study showed that superoxide radicals are likely key intermediates involved in oxidase-like catalysis. Furthermore, the porous Pt NDs exhibited excellent long-term and thermal stabilities and could be reused. Due to the exceptionally high oxide-like activity, the porous Pt NDs can catalyze the oxidation of ascorbic acid (AA) and o-phenylenediamine (OPD), forming two fluorescence emissions at 430 nm and 556 nm, respectively. A ratiometric fluorescence assay for AA and acid phosphatase (ACP) was established. The linear range for AA and ACP determination is 0.05–25 μmol·L⁻¹ and 0.1–30 U·L⁻¹, and the limit of detection was 0.036 μmol·L⁻¹ and 0.03 U·L⁻¹, respectively. Considering their high catalytic efficiency, this type of porous Pt NDs holds excellent potential for biosensor applications.

{"title":"Porous Pt nanodendrites with exceptionally high oxidase-like mimic catalytic efficiency for ratiometric fluorescence sensing of ascorbic acid and acid phosphatase","authors":"Yajun Zhong , Tiantian Xie , Jingyun Zhang , Wenyuan Liu , Qiuxiang Wang , Zhenjing Zhuang","doi":"10.1016/j.microc.2024.112277","DOIUrl":"10.1016/j.microc.2024.112277","url":null,"abstract":"<div><div>In this study, three porous platinum nanodendrites (Pt NDs) with exceptionally high catalytic efficiencies were fabricated using a carbon-dot (CD)-mediated route. This study demonstrated that the as-prepared porous Pt NDs, such as the integration of a large number of small Pt nanozymes with excellent catalytic activity, exhibited larger sizes and higher oxidase-like catalytic efficiency. The highest <em>K<sub>cat</sub></em> value of the obtained porous Pt NDs was 8.61 × 10<sup>4</sup> s<sup>−1</sup>, which was 20-fold greater than that of HRP (<em>K<sub>cat</sub></em> = 4.3 × 10<sup>3</sup> s<sup>−1</sup>). A mechanistic study showed that superoxide radicals are likely key intermediates involved in oxidase-like catalysis. Furthermore, the porous Pt NDs exhibited excellent long-term and thermal stabilities and could be reused. Due to the exceptionally high oxide-like activity, the porous Pt NDs can catalyze the oxidation of ascorbic acid (AA) and o-phenylenediamine (OPD), forming two fluorescence emissions at 430 nm and 556 nm, respectively. A ratiometric fluorescence assay for AA and acid phosphatase (ACP) was established. The linear range for AA and ACP determination is 0.05–25 μmol<strong>·</strong>L<sup>−1</sup> and 0.1–30 U<strong>·</strong>L<sup>−1</sup>, and the limit of detection was 0.036 μmol<strong>·</strong>L<sup>−1</sup> and 0.03 U<strong>·</strong>L<sup>−1</sup>, respectively. Considering their high catalytic efficiency, this type of porous Pt NDs holds excellent potential for biosensor applications.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112277"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From nanotechnology to AI: The next generation of CRISPR-based smart biosensors for infectious disease detection

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112577

Irkham , Abdullahi Umar Ibrahim , Chidi Wilson Nwekwo , Pwadubashiyi Coston Pwavodi , Salma Nur Zakiyyah , Mehmet Ozsoz , Yeni Wahyuni Hartati

Infectious diseases caused by pathogens such as viruses, bacteria, fungi and parasites continues to be a burden to global healthcare sector. Early and accurate detection of these pathogens are crucial for timely and appropriate treatment. Several conventional techniques have been developed for the detection of these diseases; however, these techniques have several trade-offs which include low accuracy and sensitivity, laborious, time consuming, expensive, multiple steps, the use of hazardous chemicals and reagent and the requirement of sophisticated devices, lack of point of care testing as well challenges in automation. The integration of nanotechnology in biosensing technology offer several advantages which include rapid, sensitive and accurate detection of pathogenic disease. The increase demand for high specific, point-of-care (POC) and deployable biosensors has led to the advent of CRISPR-based biosensors which are initially discovered in microorganism as part of their adaptive immune system and subsequently programmed as gene editing tool. CRISPR/Cas systems have become a powerful tool for the detection of infectious diseases due to its specificity. The biosensing platform relies on the specificity of Cas system couple with NA amplification techniques which include recombinase polymerase amplification (RPA), polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP) etc. The integration of smart technology into biosensing techniques can enhance efficiency and data sharing. In this review, we will briefly discuss about the general characteristics and mechanisms of the CRISPR-Cas systems, overview conventional diagnostic approaches and biosensors, nanobiosensors, smart sensing technology, CRISPR-based biosensing strategies and prospect of smart CRISPR-based biosensors for POC testing.

{"title":"From nanotechnology to AI: The next generation of CRISPR-based smart biosensors for infectious disease detection","authors":"Irkham , Abdullahi Umar Ibrahim , Chidi Wilson Nwekwo , Pwadubashiyi Coston Pwavodi , Salma Nur Zakiyyah , Mehmet Ozsoz , Yeni Wahyuni Hartati","doi":"10.1016/j.microc.2024.112577","DOIUrl":"10.1016/j.microc.2024.112577","url":null,"abstract":"<div><div>Infectious diseases caused by pathogens such as viruses, bacteria, fungi and parasites continues to be a burden to global healthcare sector. Early and accurate detection of these pathogens are crucial for timely and appropriate treatment. Several conventional techniques have been developed for the detection of these diseases; however, these techniques have several trade-offs which include low accuracy and sensitivity, laborious, time consuming, expensive, multiple steps, the use of hazardous chemicals and reagent and the requirement of sophisticated devices, lack of point of care testing as well challenges in automation. The integration of nanotechnology in biosensing technology offer several advantages which include rapid, sensitive and accurate detection of pathogenic disease. The increase demand for high specific, point-of-care (POC) and deployable biosensors has led to the advent of CRISPR-based biosensors which are initially discovered in microorganism as part of their adaptive immune system and subsequently programmed as gene editing tool. CRISPR/Cas systems have become a powerful tool for the detection of infectious diseases due to its specificity. The biosensing platform relies on the specificity of Cas system couple with NA amplification techniques which include recombinase polymerase amplification (RPA), polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP) etc. The integration of smart technology into biosensing techniques can enhance efficiency and data sharing. In this review, we will briefly discuss about the general characteristics and mechanisms of the CRISPR-Cas systems, overview conventional diagnostic approaches and biosensors, nanobiosensors, smart sensing technology, CRISPR-based biosensing strategies and prospect of smart CRISPR-based biosensors for POC testing.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112577"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fluorescent probe for selective detection of Pd2+ combined with fluorescent composite membranes and bioimaging

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112370

Haohu Li , Wen Li , Xinjie Yang , Li Liu , Haiyang Guo , Xijuan Chai , Linkun Xie , Kaimeng Xu , Guanben Du , Lianpeng Zhang

Palladium, as a transition metal of the platinum group elements, plays an indispensable role in chemical, pharmaceutical, and industrial production. However, its potential toxicity can pose risks to our health and the environment. In this work, a novel parallel alkyne-amine naphthalene ring fluorescent probe LNA based on Pd²⁺ coordination has been developed. The basic structure and detection mechanism of the probe have been confirmed by nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and liquid chromatography-mass spectrometry (LC-MS). The probe LNA has a fluorescence-off response to the Pd²⁺ with high sensitivity (as fast as 60 s) and excellent selectivity (with a detection limit as low as 0.12 μM). Due to the outstanding fluorescence properties of LNA and the low cytotoxicity of its ligand complexes, it has been successfully applied to carboxymethyl cellulose-based fluorescent composite membranes as well as Pd²⁺ imaging of living cells.

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引用次数: 0

A simple and expeditious RP-HPLC method for the simultaneous determination of commercially available seven pesticides formulations

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112292

R. Singh, J. Dhalani

For the purpose of simultaneously determination, a quick, easy, accurate and precise, high-performance liquid chromatographic analytical technique was validated for tolfenpyrad, pyraclostrobin, trifloxystrobin, azoxystrobin, fipronil, thiamethoxam, thiophanate-methyl, and azoxystrobin in their respective commercially accessible technical product compositions. Active components were separated chromatographically from additives and co-formulations using isocratic elution with 0.1 % orthophosphoric acid and acetonitrile in a 30:70 (v/v) at a flow rate of 1.0 mL per min on an Inertsil ODS-3 column (250 × 4.6 mm; 3.5 µm) with UV detection at 190 nm. With a 20-minute run time, the retention times for thiamethoxam, thiophanate-methyl, azoxystrobin, fipronil, pyraclostrobin, trifloxystrobin, and tolfenpyrad were found to be 2.96, 3.68, 5.98, 8.39, 11.31, 13.74, and 16.38 min, respectively. SANCO guidelines for precision, linearity, robustness, accuracy, LOD, specificity and selectivity were followed to approve the efficiency of this technique. Because all analyses were conducted using a single method and constant chromatographic settings, the suggested method offers several advantages, including rapid, sustainable, and eco-friendly analysis, as well as overall low costs. Developed method can be applied to water, food and different sources samples.

{"title":"A simple and expeditious RP-HPLC method for the simultaneous determination of commercially available seven pesticides formulations","authors":"R. Singh, J. Dhalani","doi":"10.1016/j.microc.2024.112292","DOIUrl":"10.1016/j.microc.2024.112292","url":null,"abstract":"<div><div>For the purpose of simultaneously determination, a quick, easy, accurate and precise, high-performance liquid chromatographic analytical technique was validated for tolfenpyrad, pyraclostrobin, trifloxystrobin, azoxystrobin, fipronil, thiamethoxam, thiophanate-methyl, and azoxystrobin in their respective commercially accessible technical product compositions. Active components were separated chromatographically from additives and co-formulations using isocratic elution with 0.1 % orthophosphoric acid and acetonitrile in a 30:70 (v/v) at a flow rate of 1.0 mL per min on an Inertsil ODS-3 column (250 × 4.6 mm; 3.5 µm) with UV detection at 190 nm. With a 20-minute run time, the retention times for thiamethoxam, thiophanate-methyl, azoxystrobin, fipronil, pyraclostrobin, trifloxystrobin, and tolfenpyrad were found to be 2.96, 3.68, 5.98, 8.39, 11.31, 13.74, and 16.38 min, respectively. SANCO guidelines for precision, linearity, robustness, accuracy, LOD, specificity and selectivity were followed to approve the efficiency of this technique. Because all analyses were conducted using a single method and constant chromatographic settings, the suggested method offers several advantages, including rapid, sustainable, and eco-friendly analysis, as well as overall low costs. Developed method can be applied to water, food and different sources samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112292"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ratiometric fluorescent probe quickly responding for Pb2+ with the biggest red-shift stokes based on a new bimetallic MOF

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-01-01 DOI: 10.1016/j.microc.2024.112333

Wei-Min Chen, Juan-Juan Shao, Fang-Ming Wang

Here firstly report a novel 3D bimetallic MOF. It can be used as a fast ratiometric fluorescent probe and fast adsorbent for Pb²⁺ ions with its greatly fluorescence enhancement and a large red-shift stokes of 166 nm, which is the biggest shift of references. Moreover, it is also the first reported MOF material that can quantitatively analyze the lanthanide metal element Tb³⁺ among LMOFs. The limits of detection are calculated as 4.12 × 10⁻⁶ M and 2.94 × 10⁻⁵ M. ICP-OES testing and calculation showed that the maximum adsorption capacity of LMOF-1 for Pb²⁺ was calculated to be 23.75 mg/g at five minutes.

引用次数: 0

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