Microchemical Journal最新文献_第10页

Potassium isotopic analysis of trace levels of K in basaltic and carbonate samples using an HF precipitation per-separation method and collison cell MC-ICP-MS 玄武岩和碳酸盐样品中痕量钾的钾同位素分析采用HF沉淀分离法和碰撞池MC-ICP-MS

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-09 DOI: 10.1016/j.microc.2025.113551

Wen-Jun Li , Shan-Ke Liu , Yan-Hong Liu , Bing-Yu Gao , Jing Wang , Ben-Xun Su

Background

Stable potassium (K) isotopes have emerged as valuable chemical tracers for a diverse range of bio-, geo-, and cosmo-chemical processes since the advent of high-precision K isotopic analysis by MC-ICP-MS. Collision cell MC-ICP-MS offers the advantage of high signal intensity (>1000 V/ppm ³⁹K), enabling the analysis of low-K samples. However, analysis of low-K samples, such as some basaltic rocks and carbonate samples, presents significant challenges due to high matrix effects, low K concentrations, and trace-level interference from Ca polyatomic ions.

Results

We developed a HF precipitation method to preconcentrate K from major matrix elements, which is then combined with a conventional small-volume resin chromatographic procedure to further purify K in carbonates and basaltic samples. The HF acid effectively removes over 95 % of Ca and Mg from carbonates while ensuring complete K recovery. For basaltic samples, the HF method successfully eliminates Al, Ca, Mg, and portions of Fe and Na, thereby enhancing the feasibility of analyzing low-K silicate samples. Although K recovery from basaltic samples ranges from 83 % to 96 %, no significant K isotopic fractionation was observed during the HF precipitation process. The robustness of this method was validated using geostandards of basalts (BHVO-2, BIR-1), dolerite (DNC-1), and coral (JCp-1), and we firstly report δ⁴¹K value for dolostone GBW07114.

Significance

This presents an HF precipitation per-separation method preconcentrate K from the major matrix elements combined with chromatographic procedures to further purify K in low-K carbonates and basaltic samples. It also demonstrates great potential for analyzing low-K and/or high-Ca (–Mg) geological samples, including those from astronomical contexts, thereby broadening the scope of K isotopic investigations into K-depleted planetary reservoirs.

自从MC-ICP-MS高精度K同位素分析出现以来，稳定的钾(K)同位素已成为多种生物、地球和宇宙化学过程中有价值的化学示踪剂。碰撞电池MC-ICP-MS具有高信号强度（>1000 V/ppm 39K）的优势，可以分析低k样品。然而，分析低钾样品，如一些玄武岩和碳酸盐岩样品，由于高基质效应、低钾浓度和微量钙多原子离子的干扰，提出了重大挑战。结果我们开发了一种HF沉淀法，从主要基质元素中预富集K，然后结合传统的小体积树脂色谱法进一步纯化碳酸盐和玄武岩样品中的K。HF酸有效地从碳酸盐中去除95% %以上的Ca和Mg，同时确保完全回收K。对于玄武岩样品，HF方法成功地消除了Al， Ca， Mg以及部分Fe和Na，从而提高了分析低钾硅酸盐样品的可行性。虽然玄武岩样品的K回收率在83 % ~ 96 %之间，但在HF沉淀过程中没有观察到明显的K同位素分馏。利用玄武岩（BHVO-2、BIR-1）、白云岩（DNC-1）和珊瑚（JCp-1）的地质标准验证了该方法的鲁棒性，并首次报道了白云岩GBW07114的δ41K值。本文提出了一种HF沉淀预分离方法，结合色谱方法从主要基质元素中预富集K，进一步纯化低钾碳酸盐和玄武岩样品中的K。它还显示了分析低钾和/或高钙（-Mg）地质样品的巨大潜力，包括那些来自天文背景的地质样品，从而扩大了钾同位素研究的范围。

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引用次数: 0

Ultrasensitive electrochemical immunosensor integrated trimetallic PtCuRu alloy and N-rich interpenetrating twin porous carbon for quantitative detection of cTnI 基于三金属PtCuRu合金和富n互穿双孔碳的超灵敏电化学免疫传感器用于cTnI的定量检测

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-09 DOI: 10.1016/j.microc.2025.113594

Xiaofei Sun , Zhaoying Wang , Shujun Wang , Shuangna Wang , Hui Jiang , Jie Zhang , Tian Liu , Qing Liu , Ping Wang , Daopeng Zhang , Kai Feng , Yueyun Li , Feng Tang

As a gold standard biomarker for acute myocardial infarction (AMI), cardiac troponin I (cTnI) plays a crucial role in the prevention and monitoring of this condition. Herein, an efficient sandwich-type electrochemical immunosensor is developed for the quantitative measurement of cTnI utilizing highly active trimetallic PtCuRu as a signal amplification platform and N-rich interpenetrating twin porous carbon (ITPC) decorated with gold nanoparticles (Au NPs/ITPC) as matrix material. The morphologically controlled ZIF-8-derived ITPC, synthesized via a facile and green method, features enlarged surface area and high nitrogen content, enabling effective H₂O₂ enrichment and Ab₁ immobilization, thereby enhancing the stability and sensitivity of the biosensor. In addition, thanks to its open structure and optimized d-orbital coupling features, the meticulously designed flower-like PtCuRu (PtCuRu NF) possess efficient mass and electron transfer ability, favoring amplifying its H₂O₂ electroreduction activity. These intrinsic advantages of the precisely fabricated electrochemical immunosensor enable outstanding sensitivity, wide dynamic linear range (10 fg mL⁻¹ to 100ng mL⁻¹) as well as ultralow detection limit (0.58 fg mL⁻¹) for cTnI. In addition, excellent reproducibility, ideal selectivity and long-term stability were also achieved for the cTnI detection. This work pioneered an innovative precisely designed method for sandwich-type electrochemical immunosensor enabling early, quantitative measurement and prognosis of cTnI.

作为急性心肌梗死（AMI）的金标准生物标志物，心肌肌钙蛋白I （cTnI）在急性心肌梗死的预防和监测中起着至关重要的作用。本文以高活性三金属PtCuRu为信号放大平台，以金纳米粒子（Au NPs/ITPC）修饰的富n互穿双孔碳（ITPC）为基体材料，开发了一种高效的三明治型电化学免疫传感器，用于cTnI的定量测量。形态可控的zif -8衍生ITPC通过简单、绿色的方法合成，具有表面积增大、含氮量高的特点，能够有效地富集H2O2和固定Ab1，从而提高了生物传感器的稳定性和灵敏度。此外，精心设计的花朵状PtCuRu （PtCuRu NF）由于其开放的结构和优化的d轨道耦合特性，具有高效的质量和电子传递能力，有利于提高其H2O2电还原活性。精密制造的电化学免疫传感器的这些固有优点使其具有出色的灵敏度，宽动态线性范围（10 fg mL - 1至100ng mL - 1）以及对cTnI的超低检测限（0.58 fg mL - 1）。此外，cTnI检测还具有良好的重现性、理想的选择性和长期稳定性。这项工作开创了一种创新的精确设计的三明治型电化学免疫传感器方法，使cTnI的早期，定量测量和预后成为可能。

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引用次数: 0

Selective and sensitive detection of metronidazole in aquatic products using a novel fluorescent sensor based on lanthanide supramolecular framework 基于镧系超分子框架的新型荧光传感器对水产品中甲硝唑的选择性灵敏检测

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-09 DOI: 10.1016/j.microc.2025.113604

Lulu Shi , Ailun Liu , Tingting Zhou , Qiqi Wang , Mei Liu , Hui Li

Metronidazole (MDZ) is a broad-spectrum antibiotic effective against bacterial and protozoan infections in both humans and poultry farms. However, excessive intake of MDZ can lead to deleterious effects. Therefore, minimizing its environmental presence is crucial for maintaining daily life. In this study, a lanthanide supramolecular framework Tb_2.67(CB[5]₂)(SIP) (1) was synthesized. Based on the intense green luminescence of 1, it was utilized as a fluorescent sensor for MDZ detection, with the limit of detection (LOD) was 0.16 μM. Further applied research on the above content has shown that this probe can be used for the detection of MDZ in fish and shrimp, with satisfactory results. By incorporating smartphone applications, the hydrogel based on 1 achieves real-time, visualization and quantification detection of MDZ, providing a user-friendly and efficient detection method. This study offers a viable alternative approach for both qualitative and quantitative assessment of MDZ, ensuring the safety of food.

甲硝唑（MDZ）是一种广谱抗生素，对人类和家禽养殖场的细菌和原生动物感染都有效。然而，过量摄入MDZ会导致有害影响。因此，最小化其环境存在对于维持日常生活至关重要。本研究合成了一种镧系超分子骨架Tb2.67(CB[5]2)(SIP)(1)。基于1的强绿色发光特性，将其作为MDZ检测荧光传感器，检测限（LOD）为0.16 μM。对上述内容的进一步应用研究表明，该探针可用于鱼虾中MDZ的检测，结果令人满意。通过结合智能手机应用，基于1的水凝胶实现了MDZ的实时、可视化、定量检测，提供了一种人性化、高效的检测方法。本研究为MDZ的定性和定量评价提供了一种可行的替代方法，确保了食品的安全性。

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引用次数: 0

Insight into potential anti-diabetic metabolites of Sycamore fig (Ficus sycomorus L.) across fruit development using metabolomics and molecular docking 利用代谢组学和分子对接研究无花果在果实发育过程中潜在的抗糖尿病代谢物

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-09 DOI: 10.1016/j.microc.2025.113597

Mai. A. Kishk , Eman Shawky , Dina A. Selim , Doaa A. Ghareeb , Ismail Celik , Reham S. Darwish , Sarah S. Takla

The metabolic profile, anti-diabetic activity, and bioactive metabolites of Ficus sycomorus L. (Sycamore fig) fruits were investigated across different developmental stages to identify potential candidates for diabetes management. UPLC/MS/MS analysis enabled the annotation of 52 compounds belonging to diverse chemical classes. Notably, unsaturated fatty acids were predominantly present in immature fruits but declined sharply as maturation commenced. Sugars were detected in the second mature stage, showing a substantial increase as fruits reached full ripeness. Alpha-amino acids and alkaloids were initially abundant in immature fruits, but their levels decreased with maturation. Interestingly, triterpenes were exclusively found in immature fruits, suggesting a stage-specific metabolic profile. OPLS-DA coefficient plots highlighted metabolites that distinguished the developmental stages, revealing patterns associated with anti-diabetic potential. Enzymatic assays demonstrated that all tested fruit samples exhibited significant inhibition of α-glycosidase and α-amylase activities. The immature fruit stage showed the strongest α-glycosidase inhibition (IC50 = 0.601 ± 0.05 µg/mL), whereas the fully ripe stage demonstrated the highest α-amylase inhibition (IC50 = 0.215 ± 0.007 µg/mL). Coefficients plots identified ten compounds positively correlated with anti-diabetic effects. Molecular docking was assessed on the ten identified metabolites, revealing rutin as the most potent α-amylase inhibitor with a score of −6.1 kcal/mol, while synephrine exhibited the highest stability against α-glycosidase (−6.7 kcal/mol). Synephrine, stearic acid, chlorogenic acid, and rutin, with the highest binding affinity to the anti-diabetic enzymes, were selected for further in-vitro assays, confirming their inhibitory activity against α-glycosidase or α-amylase. These findings position F. sycomorus fruits as promising natural sources of anti-diabetic agents, encouraging further research into their therapeutic potential.

研究了梧桐无花果（Ficus sycomorus L.）果实在不同发育阶段的代谢概况、抗糖尿病活性和生物活性代谢物，以确定潜在的糖尿病管理候选物质。通过 UPLC/MS/MS 分析，对属于不同化学类别的 52 种化合物进行了标注。值得注意的是，不饱和脂肪酸主要存在于未成熟果实中，但随着果实开始成熟，不饱和脂肪酸含量急剧下降。在第二个成熟阶段检测到了糖分，随着果实达到完全成熟，糖分大幅增加。α-氨基酸和生物碱最初在未成熟果实中含量丰富，但随着成熟度的提高，含量有所下降。有趣的是，三萜类化合物只存在于未成熟果实中，这表明了特定阶段的代谢特征。OPLS-DA 系数图突出显示了不同发育阶段的代谢物，揭示了与抗糖尿病潜力相关的模式。酶学检测表明，所有测试的水果样品都对 α-糖苷酶和 α-淀粉酶的活性有明显的抑制作用。未成熟果实阶段的α-糖苷酶抑制作用最强（IC50 = 0.601 ± 0.05 µg/mL），而完全成熟果实阶段的α-淀粉酶抑制作用最高（IC50 = 0.215 ± 0.007 µg/mL）。系数图确定了十种与抗糖尿病作用正相关的化合物。对这十种已确定的代谢物进行了分子对接评估，结果显示芦丁是最有效的α-淀粉酶抑制剂，得分为-6.1 kcal/mol，而脱氧肾上腺素对α-糖苷酶的稳定性最高（-6.7 kcal/mol）。鞘氨醇、硬脂酸、绿原酸和芦丁与抗糖尿病酶的结合亲和力最高，被选作进一步的体外试验，证实它们对α-糖苷酶或α-淀粉酶具有抑制活性。这些研究结果表明，F. sycomorus 果实是抗糖尿病药物的理想天然来源，鼓励人们进一步研究其治疗潜力。

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引用次数: 0

Research advances in centrifugal microfluidics and its applications 离心微流体及其应用研究进展

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-09 DOI: 10.1016/j.microc.2025.113589

Zhichang Du , Junpeng Lin , Beichen Lin , Shaohui Yang

Traditional microfluidic chips typically depend on bulky external pumps or power supplies to drive fluid flow, limiting their portability and suitability for field applications. In contrast, centrifugal microfluidic technology harnesses the centrifugal force generated by chip rotation to drive fluid manipulation, enabling automated processes such as valve control, mixing, distribution, and quantification. These capabilities are widely applicable in biochemical analysis and other fields. Centrifugal microfluidic systems are user-friendly, highly automated, and capable of parallel detection, providing sample-to-answer solutions and thus offering a reliable solution for on-site biochemical analysis. This review examines the development, significance of centrifugal microfluidic technology, outlines the basic mechanical principles involved, and categorizes the fluid control functions enabled by this technology. It provides an in-depth analysis of recent advances in application areas such as on-site biochemical analysis (e.g., pathogen detection), material preparation (e.g., nanoparticle synthesis), and separation (e.g., microplastic separation). Finally, it discusses the advantages and limitations of centrifugal microfluidic technology, summarizes the manufacturing technologies of chip and explores future development trends (e.g., biodegradable materials, automated detection and expansion of application fields).

传统的微流控芯片通常依赖笨重的外部泵或电源来驱动流体流动，这限制了其便携性和现场应用的适用性。相比之下，离心微流控技术利用芯片旋转产生的离心力来驱动流体操作，从而实现阀门控制、混合、分配和定量等自动化过程。这些功能广泛应用于生化分析和其他领域。离心微流控系统用户界面友好、自动化程度高、能够进行并行检测，提供从样品到答案的解决方案，从而为现场生化分析提供了可靠的解决方案。本综述探讨了离心微流控技术的发展和意义，概述了其中涉及的基本机械原理，并对该技术实现的流体控制功能进行了分类。它深入分析了现场生化分析（如病原体检测）、材料制备（如纳米粒子合成）和分离（如微塑料分离）等应用领域的最新进展。最后，它讨论了离心微流控技术的优势和局限性，总结了芯片的制造技术，并探讨了未来的发展趋势（如可生物降解材料、自动检测和应用领域的扩展）。

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引用次数: 0

Highly conductive and rapidly self-healing aqueous polyurethane ionogel skin based on ionic dipoles of IL 基于IL离子偶极子的高导电性和快速自愈水性聚氨酯离子凝胶皮肤

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-09 DOI: 10.1016/j.microc.2025.113579

Jiaqi Li , Mingxuan Yu , Haibin Niu , Chao Zhou , Li Liu , Guangfeng Wu

In today’s burgeoning e-skin trend, ionic liquids (IL) have been incorporated into polymer substrates in significant quantities due to their high conductivity and stability. However, the high substitution resistance of IL is often impeded by the polymer substrates, leading to reduced carrier mobility and sensitivity. Here, we designed a self-healing waterborne polyurethane (WPU) with a polydimethylsiloxane (PDMS) backbone and prepared WPU ion gels through complexation with IL. The increased mobility of the PDMS fragments reduces the glass transition temperature (Tg) and crystallinity of the WPU and increases the carrier transport rate of IL in the WPU substrate, thus improving the electrical conductivity. Additionally, the exchange of disulfide bonds within the backbone, along with ion–dipole interactions, facilitates the rapid repair of the ionic gel when fractured, occurring within five minutes. The resulting ionic gels possess the capability to detect subtle human motion signals, such as facial expressions, heartbeats, and muscle movements.

在当今蓬勃发展的电子皮肤趋势中，离子液体（IL）因其高导电性和稳定性已被大量融入聚合物基底中。然而，离子液体的高抗取代性往往会受到聚合物基底的阻碍，导致载流子迁移率和灵敏度降低。在这里，我们设计了一种具有聚二甲基硅氧烷（PDMS）骨架的自愈合水性聚氨酯（WPU），并通过与 IL 的复配制备了 WPU 离子凝胶。PDMS 片段流动性的增加降低了 WPU 的玻璃化转变温度（Tg）和结晶度，并提高了 IL 在 WPU 基质中的载流子传输速率，从而改善了导电性。此外，骨架内二硫键的交换以及离子-偶极子相互作用有助于离子凝胶在断裂时快速修复，修复时间不超过五分钟。由此产生的离子凝胶具有检测人体细微运动信号的能力，如面部表情、心跳和肌肉运动。

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引用次数: 0

Immobilized sorption microprobes as a tool for small-size colorimetric sensing 固定化吸附微探针作为小尺寸比色传感的工具

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-08 DOI: 10.1016/j.microc.2025.113577

Tatyana I. Tikhomirova, Vladimir V. Apyari, Sergey V. Kovalev, Ekaterina V. Nikolaeva

Miniaturized analytical procedures and tools have gained significant attention due to their advantages of low consumption of analyzed samples and reagents, low cost and high greenness. Within this field, a concept of immobilized sorption microprobes is proposed and tested. Each microprobe represents an individual sorbent particle included in an array attached to a holder via an adhesive layer. The analytical response of food dyes adsorbed from a solution on the microprobes can be directly measured using high-resolution scanning followed by processing the digital image in available software. The microprobes based on γ-Al₂O₃ and hypercrosslinked polystyrene, which differ in their polarity, were studied. An array of distanced microprobes shows higher color intensity and 3–5 times better sensitivity compared to a dense array where they are located side-by-side. The γ-Al₂O₃ microprobes were successfully applied for the micro-colorimetric determination of Ponceau-4R and Fast Green FCF synthetic food dyes in an energy drink (at the level of 2.5 µg mL⁻¹) and capsules of a pharmaceutical formulation (at the level of 6 µg per capsule cap) with the recovery within 101–115 %.

微型化分析程序和工具具有分析样本和试剂消耗量少、成本低和绿色环保等优点，因此受到了广泛关注。在这一领域，提出并测试了固定吸附微探针的概念。每个微探针代表一个单独的吸附剂颗粒，通过粘合剂层连接到一个支架上的阵列中。通过高分辨率扫描，然后用现有软件处理数字图像，就可以直接测量微探针上溶液中吸附的食品染料的分析响应。研究了基于 γ-Al2O3 和超交联聚苯乙烯的微探针，它们的极性不同。与并排排列的密集阵列相比，距离较远的微探针阵列显示出更高的颜色强度和 3-5 倍的灵敏度。γ-Al2O3微探针被成功地应用于能量饮料（2.5 µg mL-1）和药物制剂胶囊（6 µg/粒）中芳香-4R和Fast Green FCF合成食品染料的微比色测定，回收率在101-115%之间。

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引用次数: 0

Innovative analytical approach using ’on-on++ RRS’ signal enhancement response of erythrosine B, a food dye, for nano-level quantification of sertraline drug in different matrices with sustainability evaluation 利用红素B（一种食用染料）的“on-on++ RRS”信号增强反应的创新分析方法，用于不同基质中舍曲林药物的纳米级定量并进行可持续性评价

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-08 DOI: 10.1016/j.microc.2025.113585

Badriah Saad Al-Farhan , Safaa F. Saleh , Reem H. Obaydo , Angum M.M. Ibrahim , Manal A. Alossaimi , Dalin A. Hassan , Ibrahim A. Naguib , Ahmed Abdulhafez Hamad

The present study introduces a sustainable and environmentally responsible method for analyzing sertraline (STL), a selective serotonin reuptake inhibitor (SSRI) drug, emphasizing compatibility with green and white chemistry principles. A novel ultrasensitive spectrofluorimetric approach was developed, showcasing both efficiency and sustainability. This method involves a simple, single-step procedure that ensures accurate quantification of STL. A significant resonant Rayleigh scattering (RRS) enhancement was observed when STL interacted with Erythrosine B (EB), a widely used food dye, under acidic conditions. This fluorescence-based method relies on the hypothesis that the increased signal from the dye-STL complex is proportional to the molecular weight shift from 306.23 (STL) to 1186.09 (STL-EB complex), measured at 370 nm.

The method demonstrated linear detection over a range of 70–1800 ng/mL with sensitivity values between 22.51 and 68.22 ng/mL. Optimized analytical conditions for the STL-EB complex adhered to International Council for Harmonisation (ICH) standards. Human plasma and urine samples were spiked with STL drug and analyzed using a validated protocol, achieving high recovery percentages of 98.59 ± 0.94 % to 101.31 ± 1.84 % in plasma and 99.25 ± 1.25 % to 100.85 ± 1.66 % in urine, demonstrating the method’s precision and applicability to biofluids.

Environmental assessments confirmed the method’s green nature, highlighting its eco-friendly and sustainable attributes. The procedure’s simplicity, reliability, and adherence to green and white analytical principles, evaluated using eco-friendly tools, yield an Analytical Eco-scale score of 95, Mo-NEMI with all green sections, AGREE score of 0.83, complex GAPI with mostly green sections, AGREEprep score of 0.71, RGB 12 score of 95, and BAGI score of 77.5, making it highly suitable for sustainability applications across various pharmaceutical matrices.

本研究介绍了一种可持续和环保的方法来分析舍曲林（STL），一种选择性5 -羟色胺再摄取抑制剂（SSRI）药物，强调绿色和白色化学原理的相容性。开发了一种新型超灵敏荧光光谱法，展示了效率和可持续性。该方法采用简单的单步程序，可确保STL的准确定量。在酸性条件下，STL与广泛使用的食用染料红素B （EB）相互作用时，观察到明显的共振瑞利散射（RRS）增强。这种基于荧光的方法依赖于一个假设，即染料-STL络合物增加的信号与在370 nm测量的分子量从306.23 （STL）到1186.09 （STL- eb络合物）成正比。该方法在70 ~ 1800 ng/mL范围内线性检测，灵敏度在22.51 ~ 68.22 ng/mL之间。STL-EB配合物的分析条件优化符合国际协调理事会（ICH）标准。采用经验证的方法对人血浆和尿液样品进行加标分析，血浆和尿液的回收率分别为98.59±0.94% ~ 101.31±1.84%和99.25±1.25% ~ 100.85±1.66%，表明了该方法的精密度和适用性。环境评估证实了该方法的绿色性质，突出了其生态友好和可持续的属性。该程序的简单性、可靠性和对绿色和白色分析原则的遵守，使用环保工具进行评估，得出分析生态量表得分为95，Mo-NEMI所有绿色部分，AGREE得分为0.83，复杂GAPI大部分绿色部分，AGREEprep得分为0.71，RGB 12得分为95，BAGI得分为77.5，使其非常适合各种药物基质的可持续性应用。

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引用次数: 0

CRISPR/Cas12a regulated dual-channel ratiometric fluorescence biosensing for sensitive and accurate detection of kanamycin CRISPR/Cas12a调控双通道比例荧光生物传感，实现卡那霉素的灵敏、准确检测

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-08 DOI: 10.1016/j.microc.2025.113581

Xia Yi , Min Wu , Xinyue Yuwen , Jiahao Wang , Xin Li , Guosong Lai

Antibiotic residues are regarded as a serious threat to public health and safety. Traditional fluorescence biosensors with single signal-output channel are susceptible to producing false results during practical applications. In this work, a novel ratiometric fluorescent biosensor with dual signal-output channels was developed for assaying kanamycin (Kana) antibiotic residues in complex matrices. Through highly specific aptamer recognition to trigger a nuclease-catalytic DNA recycling reaction, the CRISPR/Cas12a system was activated to realize the non-discriminatory cleavage of a short signal DNA and the G-quadruplexes produced from telomerase (TE)-catalytic extension reaction. These resulted in their “signal-on” and “signal-off” fluorescence outputs, respectively, to construct the ratiometric signal transduction strategy. Due to the TE-extension and DNA recycling-based signal amplification and the highly efficient reactivity of CRISPR/Cas12a, both the sensitivity and signal output efficiency of the sensing system were effectively improved. Meanwhile, the self-correcting advantage of the dual-channel reverse signal transduction mode ensured the superior detection accuracy of the method. Based on the synergistic action of the above functional units, this biosensor was able to accurately, rapidly and sensitively detect Kana residues in the concentration range from 1 pg mL⁻¹ to 100 ng mL⁻¹ with a very low detection limit of 0.28 pg mL⁻¹. In addition, the good agreement between the measurement results of the method and an ELISA kit with the relative errors less than 5.3 % indicates its favorable practicability.

抗生素残留被视为对公众健康和安全的严重威胁。传统的单信号输出通道荧光生物传感器在实际应用中容易产生错误结果。在这项工作中，我们开发了一种具有双信号输出通道的新型比率计荧光生物传感器，用于检测复杂基质中卡那霉素（Kana）抗生素的残留量。通过高度特异性的适配体识别来触发核酸酶催化的 DNA 循环反应，CRISPR/Cas12a 系统被激活，实现了对短信号 DNA 和端粒酶（TE）催化延伸反应产生的 G-四链体的无差别切割。它们分别产生 "信号-开启 "和 "信号-关闭 "荧光输出，从而构建了比率信号转导策略。由于基于 TE 延伸和 DNA 循环的信号放大以及 CRISPR/Cas12a 的高效反应性，传感系统的灵敏度和信号输出效率都得到了有效提高。同时，双通道反向信号转导模式的自校正优势确保了该方法超高的检测精度。基于上述功能单元的协同作用，该生物传感器能够准确、快速、灵敏地检测出 1 pg mL-1 至 100 ng mL-1 浓度范围内的卡纳残留，检测限低至 0.28 pg mL-1。此外，该方法的测量结果与 ELISA 试剂盒的测量结果非常一致，相对误差小于 5.3%，这表明该方法具有良好的实用性。

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引用次数: 0

High-catalytic-activity conductive Cu-MOF coupled with carbon black Super-P for the rapid electrochemical detection of methylglyoxal in food and biological fluid 高催化活性导电铜-MOF 与碳黑 Super-P 结合用于食品和生物液体中甲基乙二醛的快速电化学检测

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-08 DOI: 10.1016/j.microc.2025.113584

Yafeng Jin , Ruilong Zhang , Xinyue Song , Didi Tong , Haibo Shang , Fangming Han , Xiaobo Li , Guangri Xu

Elevated levels of methylglyoxal (MG) in the human body are linked to various metabolic disorders. Therefore, developing efficient methods for detecting MG in food and biological fluid is of significant importance. In this work, a high-catalytic-activity conductive copper-based metal–organic framework (Cu₃(HHTP)₂) was synthesized via a hydrothermal method, and a sensitive electrochemical sensor based on a glassy carbon electrode (GCE), denoted as Super-P/Cu₃(HHTP)₂/GCE, for monitoring methylglyoxal (MG) was constructed using a composite of Cu₃(HHTP)₂ and carbon black Super-P. Leveraging the catalytic activity of Cu₃(HHTP)₂ and the enhanced conductivity provided by Super-P, the sensor demonstrated a wide linear detection range (1–500 µM) and a low detection limit (0.0275 µM). Moreover, it was successfully applied to the determination of MG in milk, beer, urine, and human serum samples, achieving recoveries ranging from 91.7 % to 115.7 %. This work opens up new avenues for the development of innovative electroanalytical tools for MG monitoring in both food and biological fluid.

人体内甲基乙二醛（MG）水平升高与各种代谢紊乱有关。因此，开发食品和生物流体中MG的高效检测方法具有重要意义。本文采用水热法合成了具有高催化活性的导电铜基金属有机骨架（Cu3(HHTP)2），并用Cu3(HHTP)2和炭黑Super-P复合材料构建了基于玻碳电极（GCE）的敏感电化学传感器Super-P/Cu3(HHTP)2/GCE，用于监测甲基乙二醛（MG）。利用Cu3(HHTP)2的催化活性和Super-P提供的增强电导率，该传感器具有宽的线性检测范围（1-500µM）和低的检测限（0.0275µM）。该方法可用于牛奶、啤酒、尿液和人血清样品中MG的测定，回收率为91.7% ~ 115.7%。这项工作为开发用于食品和生物流体中MG监测的创新电分析工具开辟了新的途径。

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