Microchemical Journal最新文献_第2页

Enhanced sensitivity electrochemical sensor for melatonin detection using Antibody-Anchored RGO and silver nanoparticles

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-18 DOI: 10.1016/j.microc.2025.113702

Xiali Guan , Gang Zhou , Yubo Fan

Melatonin (MT) is a hormone regulating the sleep-wake cycle and serves as a potential biomarker for various physiological and pathological conditions. Traditional MT detection methods, such as HPLC and MS, face challenges including complex processing, low sensitivity, and the inability to provide real-time monitoring, limiting their clinical applicability. Electrochemical sensors offer advantages like simplicity, high sensitivity, and real-time detection; however, issues such as insufficient selectivity, poor stability, and limited reproducibility in complex biological matrices persist. To address these challenges, we developed an Ab-MEL/AR/ITO electrochemical sensor by integrating AgNPs@RGO (AR) composites, synthesized using a dopamine in situ reduction method, with the MEL-1A-R (B-10) antibody (Ab-MEL) as a selective molecular recognition element for MT detection. The sensor exhibited a linear response to MT concentrations ranging from 0.01 to 1000 μM, with an exceptionally low limit of detection (LOD) of 0.0016 μM. It demonstrated excellent selectivity, maintaining performance in the presence of interferents like NaCl, glucose, and dopamine, and exhibited high reproducibility and long-term stability, with only a 5.68 % decrease in response after 28 days. The sensor achieved recovery rates of 98.10 % to 106.30 % in synthetic urine, human serum, and commercial MT capsules, underscoring its robustness and precision. By addressing the limitations of conventional methods, this Ab-MEL/AR/ITO sensor provides a highly sensitive, selective, and stable platform for MT detection, offering significant potential for clinical diagnostics and biomedical research applications.

{"title":"Enhanced sensitivity electrochemical sensor for melatonin detection using Antibody-Anchored RGO and silver nanoparticles","authors":"Xiali Guan , Gang Zhou , Yubo Fan","doi":"10.1016/j.microc.2025.113702","DOIUrl":"10.1016/j.microc.2025.113702","url":null,"abstract":"<div><div>Melatonin (MT) is a hormone regulating the sleep-wake cycle and serves as a potential biomarker for various physiological and pathological conditions. Traditional MT detection methods, such as HPLC and MS, face challenges including complex processing, low sensitivity, and the inability to provide real-time monitoring, limiting their clinical applicability. Electrochemical sensors offer advantages like simplicity, high sensitivity, and real-time detection; however, issues such as insufficient selectivity, poor stability, and limited reproducibility in complex biological matrices persist. To address these challenges, we developed an Ab-MEL/AR/ITO electrochemical sensor by integrating AgNPs@RGO (AR) composites, synthesized using a dopamine in situ reduction method, with the MEL-1A-R (B-10) antibody (Ab-MEL) as a selective molecular recognition element for MT detection. The sensor exhibited a linear response to MT concentrations ranging from 0.01 to 1000 μM, with an exceptionally low limit of detection (LOD) of 0.0016 μM. It demonstrated excellent selectivity, maintaining performance in the presence of interferents like NaCl, glucose, and dopamine, and exhibited high reproducibility and long-term stability, with only a 5.68 % decrease in response after 28 days. The sensor achieved recovery rates of 98.10 % to 106.30 % in synthetic urine, human serum, and commercial MT capsules, underscoring its robustness and precision. By addressing the limitations of conventional methods, this Ab-MEL/AR/ITO sensor provides a highly sensitive, selective, and stable platform for MT detection, offering significant potential for clinical diagnostics and biomedical research applications.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113702"},"PeriodicalIF":4.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances of capillary electrophoresis for enzyme analysis (2021–2024)

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-18 DOI: 10.1016/j.microc.2025.113701

Yangyang Su , Fan Shui , Jia Tang, Yanping Wei, Qian Zhang, Famin Ke, Jing Zeng

Capillary electrophoresis (CE) is a robust and versatile analytical technique that has demonstrated significant potential and emerging trends in enzyme analysis. This review comprehensively summarizes the latest advancements in CE for enzyme analysis from 2021 to 2024, covering both homogeneous and heterogeneous analysis. The advancements in homogeneous CE enzyme analysis, including both pre-capillary enzyme analysis and in-capillary enzyme analysis, are thoroughly examined. Specifically, an in-depth analysis is provided of the general principles, characteristics, and key parameters at each step of the pre-capillary analysis process, such as incubation, termination, separation, and detection. Moreover, this review introduces various novel developments and applications in different mixed modes of in-capillary enzyme analysis. Additionally, it discusses diverse immobilization methods, such as adsorption, cross-linking, covalent bonding, encapsulation, and the recently developed nanogels method, aiming to construct highly efficient and stable immobilized enzyme microreactors (IMERs). This review presents an up-to-date overview of CE applications in enzyme analysis, highlighting its significant potential in this field.

{"title":"Recent advances of capillary electrophoresis for enzyme analysis (2021–2024)","authors":"Yangyang Su , Fan Shui , Jia Tang, Yanping Wei, Qian Zhang, Famin Ke, Jing Zeng","doi":"10.1016/j.microc.2025.113701","DOIUrl":"10.1016/j.microc.2025.113701","url":null,"abstract":"<div><div>Capillary electrophoresis (CE) is a robust and versatile analytical technique that has demonstrated significant potential and emerging trends in enzyme analysis. This review comprehensively summarizes the latest advancements in CE for enzyme analysis from 2021 to 2024, covering both homogeneous and heterogeneous analysis. The advancements in homogeneous CE enzyme analysis, including both pre-capillary enzyme analysis and in-capillary enzyme analysis, are thoroughly examined. Specifically, an in-depth analysis is provided of the general principles, characteristics, and key parameters at each step of the pre-capillary analysis process, such as incubation, termination, separation, and detection. Moreover, this review introduces various novel developments and applications in different mixed modes of in-capillary enzyme analysis. Additionally, it discusses diverse immobilization methods, such as adsorption, cross-linking, covalent bonding, encapsulation, and the recently developed nanogels method, aiming to construct highly efficient and stable immobilized enzyme microreactors (IMERs). This review presents an up-to-date overview of CE applications in enzyme analysis, highlighting its significant potential in this field.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113701"},"PeriodicalIF":4.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-sensitive determination of Zn, Cd, Ni, Cu, and Pb in high salt matrix water samples using solid phase extraction coupled with solution cathode glow discharge atomic emission spectrometry

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-18 DOI: 10.1016/j.microc.2025.113695

Meng Gao , Jihui Shi , Qing Li , Zheng Wang

Herein, we developed a highly sensitive method based on solution cathode glow discharge atomic emission spectrometry (SCGD-AES) coupled with solid-phase extraction (SPE) for the determination of Zn, Cd, Ni, Cu, and Pb in high salt matrix water samples. The trace metals were collected from 100 mL water which was adjusted the pH to 4–10 (this step was unnecessary for most environmental water samples due to their inherent pH compatibility) using a column (i.d. = 6.6 mm) of a chelating resin (bed height = 7.2 cm) and eluted with 5 mL 1 M HNO₃ at a flow rate of 3 mL/min. At the same time, the pH of the eluate would be adjust from 0 to 0.9 (a suitable acidity for SCGD) by the resin column. Then the heavy metals in elute were detected by SCGD with constant current mode. When 100 mL sample water was enriched, the detection limits of Zn, Cd, Ni, Cu, and Pb were 1, 0.6, 2, 0.6, and 6 μg/ L which were improved by almost 20 times. And the tolerance of SCGD for Cd, Cu, Ni, Pb, and Zn detection against the salt matrix (exemplified by Na) could reach up to 10000 mg/L. The method’s accuracy and practicality were validated through successful application to environmental water samples, high salt matrix simulated water and certified reference materials (GBW08608).

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引用次数: 0

One-pot target-triggered catalytic DNA assembly for PEC nucleic acid assay based on GDY/CuSe modified electrode

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.microc.2025.113676

Shenghao Zhang, Meiling Zhang, Yinhao Zhang, Yingxia Zong, Xu Hun

Here, we designed a catalytic DNA assembly (CDA) amplification strategy to construct a photoelectrochemical (PEC) assay to detect the specific target F3L-gene of Monkeypox Virus (MPXV). Graphdiyne (GDY) and CuSe were synthesized by a surface-assisted deprotection/polymerization method and hydrothermal method, respectively. Then AuNPs and GDY/CuSe nanocomposites were modified on the surface of gold electrode (GE). The hairpin DNA4 (H4) was incubated onto the surface of the electrode by Au-S bond. When the target was present, the CDA was occurred by hairpin DNA1 (H1) and hairpin DNA2 (H2). Then, DNA3 (H3) hybridized with the single stranded region of H1-H2 complex, formed a site that can be hydrolyzed by exonuclease III, led to exonuclease III hydrolysis of DNA and to release the H3 primer (H3P). H3 further opened H4 and triggered a subsequent CDA reaction, ultimately formed a Y-shaped DNA structure on the electrode surface with the help of DNA5 (H5) and DNA6 (H6). The two end sequences of H4 and H6, which constituted the Y-shaped structure, were designed as split H3P fragments. They can be used as primers to trigger CDA reactions for more efficient and characteristic autocatalytic signal amplification. The Y-shaped structure carried H5-ALP, and catalyzed phosphonoxyphenol (o-PP) to generate benzene-1,2-diol (CA), which could enhance the PEC signal. The linear range is 0.1 fM-10.0 pM with the detection limit of 0.05 fM (3S/N) for MPXV.

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引用次数: 0

Two lateral flow immunoassay assays based on different nanoparticle probes for the on-site detection of Streptococcus pyogenes in raw milk

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.microc.2025.113689

Qianxin Li , Jingfeng Zhang , Tingting Jiang , Longbo Yu , Fuping Zeng , Lichao Zhao

Mastitis, a common disease in dairy cows, impacts both dairy cows health and farm profitability. Streptococcus pyogenes, a major pathogen, can cause outbreaks in cows and foodborne illnesses, threatening dairy product safety. Given the specificity of the cows farm location and limited testing resources and possible missed detections due to the presence of several different subtypes of S. pyogenes, two rapid tests were developed: a latex beads-based lateral flow immunoassay (LBs-LFIA) and a fluorescent immunoassay using europium nanoparticles (EuNPs-LFIA). LBs-LFIA can detect S. pyogenes in raw milk at 10⁵ CFU/mL within 15 min without complex pre-treatment, aiding in mastitis diagnosis. EuNPs-LFIA offers quantifiable detection with a sensitivity of 5 × 10³ CFU/mL. In field tests, these methods detected S. pyogenes in 4.62 % (9/195) of raw milk samples, aligning with standard methods. These tools provide effective on-site detection options for dairy product safety and contribute to dairy cow health monitoring.

乳腺炎是奶牛的常见病，对奶牛健康和牧场收益都有影响。化脓性链球菌是一种主要病原体，可导致奶牛疾病爆发和食源性疾病，威胁乳制品安全。考虑到奶牛场位置的特异性和有限的检测资源，以及由于存在多种不同亚型的化脓性链球菌而可能造成的漏检，我们开发了两种快速检测方法：基于乳胶珠的侧流免疫分析法（LBs-LFIA）和使用铕纳米颗粒的荧光免疫分析法（EuNPs-LFIA）。LBs-LFIA 可在 15 分钟内检测出生乳中 105 CFU/mL 的化脓性链球菌，无需复杂的预处理，有助于乳腺炎的诊断。EuNPs-LFIA 可进行定量检测，灵敏度为 5 × 103 CFU/mL。在现场测试中，这些方法在 4.62 %（9/195）的生乳样本中检测到了化脓性链球菌，与标准方法一致。这些工具为乳制品安全提供了有效的现场检测选择，有助于奶牛健康监测。

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引用次数: 0

Recent advances in photo/electrochemical biosensing of chemical food contaminants based on the porphyrin-MOFs nanohybrids

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.microc.2025.113671

Ebraheem Abdu Musad Saleh , M.M. Moharam , Majid S. Jabir , Irfan Ahmad , R. Roopashree , Aditya Kashyap , T. Krithiga , Subhashree Ray , Ibrahim Abbas Mohammed , Hassan Abdulhadi Jasim

The issue of food safety has become a global challenge to public health due to the presence of various contaminants. Photo/electrochemical biosensing technology has shown significant potential for application in food safety and has increasingly emerged as a research hotspot. New materials with controllable structures that are eco-friendly and exhibit high photo/electroactivity, are highly desirable. The incorporation of porphyrin molecules as fundamental components (ligands) in the construction of metal–organic frameworks (MOFs) results in the formation of porphyrinic MOFs, which can also integrate functional materials within these frameworks. Conversely, the entrapment of porphyrin molecules within porous MOFs leads to the formation of structures referred to as porphyrin@MOFs. This integration can effectively enhance the photoelectric properties of porphyrins and mitigate their tendency for self-aggregation. Hence, these materials are extremely preferred in photo/electrochemical detecting platforms. This review aims to examine the strengths and weaknesses of porphyrin-MOFs nanohybrids, as well as their mechanisms in photoelectrochemical sensing, electrochemical sensing, optical sensing, and electrochemiluminescence sensing. Moreover, this paper offers a comprehensive overview of recent advancements in porphyrin-MOFs platforms for the detection of pesticides, antibiotics, heavy metal ions, and mycotoxins, highlighting their multiplexing capabilities and sensitivity. Additionally, the limitations and challenges associated with the application of porphyrin-MOF nanohybrids in the efficient and precise assessment of food are also discussed.

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引用次数: 0

High-throughput profiling of sweet potato vine biomass for cellulosic ethanol production using near-infrared spectroscopy and chemometrics

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.microc.2025.113679

Chaochen Tang , Meng Li , Bingzhi Jiang , Irsa Ejaz , Asif Ameen , Xueying Mo , Meixian Zhi , Zhangying Wang

Sweet potato (Ipomoea batatas L.) vines, despite their abundance as agricultural by-products, remain underexplored as lignocellulosic feedstocks for bioethanol production. To address this gap, the present study introduces a novel high-throughput phenotyping strategy that integrates near-infrared spectroscopy (NIRS) with chemometrics to rapidly evaluate the bioethanol potential of sweet potato vine biomass. A diverse panel of 115 germplasm accessions was analyzed to develop robust quantitative and qualitative NIRS models. Seven optimized partial least squares regression (PLSR) models, encompassing cellulose, hemicellulose, lignin, soluble sugar, hexose, pentose, and theoretical ethanol potential (TEP), exhibited exceptional accuracy, with determination coefficients (R²) of 0.92–0.96 (calibration), 0.90–0.95 (cross-validation), and 0.87–0.94 (external validation). The ratio of prediction to deviation (RPD) values ranged from 5.64 to 8.33, confirming strong predictive capacity. Additionally, a complementary partial least squares-discriminant analysis (PLS-DA) model achieved 98% calibration accuracy and 93% validation accuracy in classifying feedstock quality grades, enabling efficient germplasm screening. This study, for the first time, demonstrates that NIRS-based phenotyping can replace traditional wet chemistry methods for large-scale evaluation of sweet potato vine biomass. Our approach provides a paradigm for accelerating the development of dedicated bioenergy crops through rapid trait profiling and precision breeding.

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引用次数: 0

Raman micro-spectroscopy uncovers complex structural and chemical adaptations of alpine azalea leaf surface

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.microc.2025.113690

Giuseppe Tiloca , Gilbert Neuner , Reinhard Jetter , Notburga Gierlinger

Cuticles are layered multicomponent structures covering plant surfaces. They have numerous functions, and their composition varies between species, plant organs and even within cuticle layers. Therefore, microchemical methods are needed to investigate cuticle structures with the necessary spatial and chemical resolution. Raman micro-spectroscopy allows such non-destructive, in-situ analysis with a lateral resolution of 300 nm. After cutting microsections from the alpine azalea (Kalmia procumbens) leaves, we acquired position-resolved Raman spectra from the adaxial and abaxial surfaces (including trichomes and stomata). Multivariate data analysis of the hyperspectral datasets revealed various cuticle layers and the epidermal cell walls, with characteristic composition of alkanes, triterpenoids, phenolics and carbohydrates. Alkanes were found as thin cuticle layers and inside cells at the basis of trichomes, while triterpenoids were present in diverse cuticle locations in varying amounts and compositions. In particular, amyrins were detected on the abaxial cuticle, while ursolic and oleanolic acid impregnated the adaxial cuticle and the distal part of the trichomes. On the adaxial side of the leaf, flavonoids were detected throughout the cuticle and as clusters within the epidermal cell lumen as well as tiny spines on the outer surface. In contrast, flavonoids were less prominent in the abaxial cuticle, but characteristic Raman bands could instead be attributed to cinnamic acids. The trichomes and thick cuticle across both leaf surfaces, and their impregnation with triterpenoids and aromatic components may help K. procumbens withstand the extreme environmental conditions of its habitat.

{"title":"Raman micro-spectroscopy uncovers complex structural and chemical adaptations of alpine azalea leaf surface","authors":"Giuseppe Tiloca , Gilbert Neuner , Reinhard Jetter , Notburga Gierlinger","doi":"10.1016/j.microc.2025.113690","DOIUrl":"10.1016/j.microc.2025.113690","url":null,"abstract":"<div><div>Cuticles are layered multicomponent structures covering plant surfaces. They have numerous functions, and their composition varies between species, plant organs and even within cuticle layers. Therefore, microchemical methods are needed to investigate cuticle structures with the necessary spatial and chemical resolution. Raman micro-spectroscopy allows such non-destructive, <em>in-situ</em> analysis with a lateral resolution of 300 nm. After cutting microsections from the alpine azalea (<em>Kalmia procumbens</em>) leaves, we acquired position-resolved Raman spectra from the adaxial and abaxial surfaces (including trichomes and stomata). Multivariate data analysis of the hyperspectral datasets revealed various cuticle layers and the epidermal cell walls, with characteristic composition of alkanes, triterpenoids, phenolics and carbohydrates. Alkanes were found as thin cuticle layers and inside cells at the basis of trichomes, while triterpenoids were present in diverse cuticle locations in varying amounts and compositions. In particular, amyrins were detected on the abaxial cuticle, while ursolic and oleanolic acid impregnated the adaxial cuticle and the distal part of the trichomes. On the adaxial side of the leaf, flavonoids were detected throughout the cuticle and as clusters within the epidermal cell lumen as well as tiny spines on the outer surface. In contrast, flavonoids were less prominent in the abaxial cuticle, but characteristic Raman bands could instead be attributed to cinnamic acids. The trichomes and thick cuticle across both leaf surfaces, and their impregnation with triterpenoids and aromatic components may help <em>K. procumbens</em> withstand the extreme environmental conditions of its habitat.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113690"},"PeriodicalIF":4.9,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple and rapid quantification of deuterium isotopologues in a deuterated drug substance deuremidevir using Fourier-transform infrared spectroscopy and partial least squares

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.microc.2025.113680

Yiwen Huang , Zhong Li , Rong Chen , Yefei Qian , Hui Lu , Yang Wu

Deuterated drugs are increasingly becoming a prominent focus in drug design and development. The level of deuterium isotopologues (isotopic impurities) is the critical quality attribute of deuterated active pharmaceutical ingredients (APIs), especially for those synthesized by isotopic exchange approaches. However, existing analytical strategies rely on high-end instruments such as LC-MS and NMR, which are not routinely equipped in quality control (QC) laboratories. Herein, we developed a QC-friendly quantitative method for determining the deuterium isotopologue in deuremidevir hydrobromide (VV116), a deuterated drug substance synthesized by isotope exchange, using Fourier-transform infrared (FTIR) spectroscopy integrated with partial least squares (PLS) regression. A gradient series of solid mixtures with defined levels of the deuterium isotopologue was designed for FTIR acquisition, and the quantitative model was optimized through a combinatorial strategy that simultaneously screens various spectral preprocessing and wavenumber variables. Ultimately, a PLS model with max–min normalization preprocessing and wavenumber variables selected using backward interval PLS followed by competitive adaptive reweighted sampling offered optimal performance, and could be directly applied for the rapid and straightforward release testing of VV116 in QC laboratories. This work provides a simpler alternative to complex analytical techniques for deuterium isotopologues determination in a deuterated API.

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引用次数: 0

Synergistic effect of MOF@Pt@MOF-B(OH)2 in biosensing platform: The combined action of boronic acid groups and composite MOF applied to dual-mode detection and portable smartphone sensing of bacteria in food

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.microc.2025.113685

Qijun He , Bin Du , Wei Hu , Lingxiao Liu , Bing Liu , Xihui Mu , Zhaoyang Tong , Rabigul Tursun , Jianjie Xu

Bacterial contamination in food constitutes a grave menace to the global public health. Consequently, the rapid detection of foodborne bacteria is of paramount importance for addressing the increasingly severe challenges. Herein, a multifunctional MOF@Pt@MOF-B(OH)₂ was developed, integrating colorimetric-fluorescence detection and smartphone sensing to create a novel biosensing platform for the detection of Escherichia coli (E. coli) in food. The MOF@Pt@MOF-B(OH)₂ exhibited exceptional enzymatic activity and fluorescence property owing to its composite structure, as well as its ability to recognize bacterial glycoproteins through boronic acid groups. This enabled selective identification of glycosylated sites on the surface of E. coli through covalent interactions, thereby amplifying the detection signal. The colorimetric-fluorescence platform achieved selective detection of E. coli, boasting a detection limit of 2 and 1 CFU/mL within a linear detection range of 1.5 × 10¹–1.5 × 10⁴ CFU/mL. This dual-mode strategy demonstrated remarkable specificity and interference resistance, achieving good recovery rates (91.76 %–108.73 %) in food samples with a relative standard deviation (RSD) below 4.72 %. Additionally, recognizing the importance of portable detection for on-site monitoring, a smartphone-mediated portable detection device was developed for real-time use. This device similarly could detect E. coli with high sensitivity and showed excellent practical application potential, achieving recovery rates from 95.57 % to 98.61 %, with RSD values below 2.56 %. These results fully demonstrated the effectiveness of the sensing platform for detecting E. coli in food.

{"title":"Synergistic effect of MOF@Pt@MOF-B(OH)2 in biosensing platform: The combined action of boronic acid groups and composite MOF applied to dual-mode detection and portable smartphone sensing of bacteria in food","authors":"Qijun He , Bin Du , Wei Hu , Lingxiao Liu , Bing Liu , Xihui Mu , Zhaoyang Tong , Rabigul Tursun , Jianjie Xu","doi":"10.1016/j.microc.2025.113685","DOIUrl":"10.1016/j.microc.2025.113685","url":null,"abstract":"<div><div>Bacterial contamination in food constitutes a grave menace to the global public health. Consequently, the rapid detection of foodborne bacteria is of paramount importance for addressing the increasingly severe challenges. Herein, a multifunctional MOF@Pt@MOF-B(OH)<sub>2</sub> was developed, integrating colorimetric-fluorescence detection and smartphone sensing to create a novel biosensing platform for the detection of Escherichia coli (<em>E. coli</em>) in food. The MOF@Pt@MOF-B(OH)<sub>2</sub> exhibited exceptional enzymatic activity and fluorescence property owing to its composite structure, as well as its ability to recognize bacterial glycoproteins through boronic acid groups. This enabled selective identification of glycosylated sites on the surface of <em>E. coli</em> through covalent interactions, thereby amplifying the detection signal. The colorimetric-fluorescence platform achieved selective detection of <em>E. coli</em>, boasting a detection limit of 2 and 1 CFU/mL within a linear detection range of 1.5 × 10<sup>1</sup>–1.5 × 10<sup>4</sup> CFU/mL. This dual-mode strategy demonstrated remarkable specificity and interference resistance, achieving good recovery rates (91.76 %–108.73 %) in food samples with a relative standard deviation (RSD) below 4.72 %. Additionally, recognizing the importance of portable detection for on-site monitoring, a smartphone-mediated portable detection device was developed for real-time use. This device similarly could detect <em>E. coli</em> with high sensitivity and showed excellent practical application potential, achieving recovery rates from 95.57 % to 98.61 %, with RSD values below 2.56 %. These results fully demonstrated the effectiveness of the sensing platform for detecting <em>E. coli</em> in food.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113685"},"PeriodicalIF":4.9,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0