Microchemical Journal最新文献_第2页

High-sensitivity label-free electrochemical genosensors for carbon nanotube plasmon-assisted detection of somatic mutations in nucleic acids from formalin-fixed paraffin-embedded tissues 高灵敏度无标记电化学基因传感器用于碳纳米管等离子体辅助检测福尔马林固定石蜡包埋组织中核酸的体细胞突变

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-26 DOI: 10.1016/j.microc.2024.112234

V.P. Egorova , H.V. Grushevskaya , N.G. Krylova , E.V. Vaskovtsev , A.S. Babenka , I.V. Anufreyonak , S.Yu. Smirnov , G.G. Krylov

Currently, molecular genetic testing of somatic point mutations in the genome of cancer tumors for targeted therapy requires high-performance tools for studies of spatial gene expression and spatial genome profiling in formalin-fixed paraffin-embedded (FFPE) tissue samples. We offer new high-performance label-free electrochemical impedimetric deoxyribonucleic-acid (DNA) nanosensors. The sensors based on a platform of crystalline carbon nanotube plasmonic assemblies are fabricated by the Langmuir–Blodgett deposition technique. The carbon-nanotube assemblies are suspended on nanoporous supports. Incorporation of fast Fourier transforms in the impedance spectroscopy led to the introduction of a new information parameter, the integral value of capacitance changes. The allele-sensitive assay based on plasmon-assisted effects in the assemblies of electrically active conjugates between DNA and few-walled carbon nanotubes has been used for discrimination of allele single-nucleotide polymorphisms (SNPs) of Kirsten Rat Sarcoma viral oncogene homologue (KRAS gene). Using 19- and 20-mer single-stranded (ss) probe oligonucleotides and 35- and 47-mer toehold probes, we explored the probe length and the target location. The genotyping technology allows discrimination of single-nucleotide variations in the target 35-base oligonucleotide at the attomolar concentration level. Human genomic DNAs were isolated from FFPE colorectal cancer tumor tissue samples. The KRAS-gene exon 2, codon 12, c.35G

>

A mutation has been successfully discriminated in the genomic DNAs with a significance level, P, of 0.001–0.02. The assay has a sensitivity with 0.33-ng/ml limit of detection (LOD) for native genomic DNA.

目前，针对靶向治疗的肿瘤基因组体细胞点突变的分子遗传学检测需要高性能的工具来研究福尔马林固定石蜡包埋（FFPE）组织样本中的空间基因表达和空间基因组谱。我们提供新的高性能无标签电化学阻抗法脱氧核糖核酸（DNA）纳米传感器。基于晶体碳纳米管等离子体组件平台的传感器采用Langmuir-Blodgett沉积技术制备。碳纳米管组件悬浮在纳米孔支架上。在阻抗谱中引入快速傅里叶变换，引入了一个新的信息参数，即电容变化的积分值。基于等离子体辅助作用的DNA与碳纳米管电活性偶联物组装的等位基因敏感检测已被用于Kirsten大鼠肉瘤病毒癌基因同源物（KRAS基因）等位基因单核苷酸多态性（snp）的鉴别。利用19-和20-mer单链（ss）探针寡核苷酸和35-和47-mer脚探针，我们探索了探针长度和目标位置。基因分型技术允许在原子摩尔浓度水平上对目标35碱基寡核苷酸的单核苷酸变异进行区分。从FFPE结直肠癌肿瘤组织样本中分离出人类基因组dna。kras基因外显子2，密码子12，c.35G>；A突变在基因组dna中被成功区分，显著性水平P为0.001-0.02。该方法对本地基因组DNA的检测限为0.33 ng/ml。

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引用次数: 0

Colorimetric reversibility: Aqueous phase recognition of cyanide using smart phone-based device with real sample analysis 比色可逆性：使用基于智能手机的设备与真实样品分析氰化物的水相识别

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-26 DOI: 10.1016/j.microc.2024.112259

Jyoti Jangir , Kiran , Anju Ranolia , Priyanka , Sandhya Chahal , Snigdha Singh , Anil Duhan , Rahul Kumar Dhaka , Devender Singh , Parvin Kumar , Jayant Sindhu

A novel colorimetric 2-hydrazinobenzothiazole based probe (BTHP) was successfully synthesized and employed as an optical cyanide sensor. The interaction of CN⁻ with BTHP produced a distinct color variation (dark pink color) with a red shift (96 nm) in its absorption spectra. The probe was selective, sensitive and robust towards cyanide ion sensing even in the presence of other competing ions. It could detect cyanide ion upto 0.77 µM, even lower than the limit recommended by WHO (1.9 µM). The binding stoichiometry for BTHP-CN⁻ complex (1:1) was confirmed via Job’s plot. A binding constant of 3.19*10² M⁻¹ was calculated in the recognition process. The cyanide ion recognition mechanism was confirmed using ¹H NMR titration and DFT calculation. The probe coated filter paper test strips displayed dark pink color upon cyanide exposure. BTHP can be used reversibly for the visible detection of cyanide in liquid (real water) as well as solid phase (soil). Further, the practical applications of the developed sensor include smartphone based sensing, logic gate fabrication, molecular keypad lock and write-read-erase-read devices.

成功合成了一种新型比色2-肼苯并噻唑基探针（BTHP），并将其用作光学氰化物传感器。CN−与BTHP的相互作用产生了明显的颜色变化（深粉红色），其吸收光谱红移（96 nm）。即使在存在其他竞争离子的情况下，该探针对氰化物离子也具有选择性，灵敏度和鲁棒性。检测氰化物离子最高可达0.77µM，甚至低于WHO推荐的1.9µM限值。BTHP-CN -配合物的结合化学计量（1:1）通过Job’s plot得到证实。在识别过程中计算出的结合常数为3.19*102 M−1。通过1H NMR滴定和DFT计算证实了氰化物离子识别机理。探针涂布的滤纸测试条在氰化物暴露后显示深粉红色。BTHP可以可逆地用于液体（真正的水）以及固相（土壤）中氰化物的可见检测。此外，所开发的传感器的实际应用包括基于智能手机的传感、逻辑门制造、分子键盘锁和写-读-擦-读器件。

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引用次数: 0

Dispersive solid phase extraction and quadruple isotope dilution–mass spectrometry combination for the accurate and sensitive quantification of capsaicin in pepper, domestic wastewater and human saliva samples by GC–MS system 分散固相萃取-四同位素稀释-质谱联用GC-MS系统准确、灵敏地定量辣椒、生活废水和人唾液样品中的辣椒素

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-23 DOI: 10.1016/j.microc.2024.112246

Süleyman Bodur , Sezin Erarpat Bodur , Selim Gürsoy , Merve Fırat Ayyıldız , Bedrihan Kartoğlu , Hilal Akbıyık , Ömer Tahir Günkara , Sezgin Bakırdere

In the presented study, reduced graphene oxide/Fe₃O₄ (rGO/Fe₃O₄) nanocomposites based dispersive solid phase extraction (DSPE) – gas chromatography–mass spectrometry (GC–MS) method was developed for the determination of capsaicin in domestic wastewater (DW), pepper (PP) and human saliva (HS) samples. All important parameters of the DSPE method affected the preconcentration factor were carefully optimized to achieve high signal to noise ratio for the analyte. After the optimization studies, the system analytical performance of DSPE-GC–MS system was evaluated using the aqueous standard solution of capsaicin. Limit of detection (LOD), limit of quantitation (LOQ) and dynamic range were figured out to be 0.54 µg/kg, 1.80 µg/kg and 2.66 – 487.35 µg/kg, respectively. Under the optimum experimental conditions, recovery studies were conducted with the spiked DW, PP and HS samples, and percent recovery results were recorded between 52.6 % and 183.6 % via matrix matching calibration strategy. After the implementation of ID⁴ strategy, percent recovery results for the spiked DW, PP and HS samples were calculated as 98.2 %–99.3 %, 99.7 %–100.7 % and 99.4 %–99.8 %, respectively. In addition, capsaicin content in Sivri (S)-PP, Kıl (K)-PP and Samandağ (SA)-PP samples were found to be 309.5 ± 11.8 mg/kg, 873.7 ± 26.7 mg/kg and 165.3 ± 5.1 mg/kg via DSPE-GC-ID⁴-MS method, respectively. As a result, the combination of quadruple isotope dilution (ID⁴) strategy and the DSPE-GC–MS method were successfully performed to boost the accuracy and precision of developed DSPE-GC–MS method.

本研究建立了还原氧化石墨烯/Fe3O4 （rGO/Fe3O4）纳米复合材料分散固相萃取(DSPE) -气相色谱-质谱（GC-MS）法测定生活废水（DW）、辣椒（PP）和人唾液（HS）样品中的辣椒素。对影响预富集系数的各重要参数进行了优化，得到了高信噪比的分析物。通过优化研究，以辣椒素为标准水溶液，评价了DSPE-GC-MS系统的分析性能。检测限（LOD）为0.54µg/kg，定量限（LOQ）为1.80µg/kg，动态范围为2.66 ~ 487.35µg/kg。在最佳实验条件下，对添加的DW、PP和HS样品进行了回收率研究，通过矩阵匹配校准策略，回收率在52.6% ~ 183.6%之间。采用ID4策略后，DW、PP和HS样品的加标回收率分别为98.2% ~ 99.3%、99.7% ~ 100.7%和99.4% ~ 99.8%。DSPE-GC-ID4-MS法测定Sivri (S)-PP、Kıl (K)-PP和samandaku (SA)-PP样品中辣椒素含量分别为309.5±11.8 mg/kg、873.7±26.7 mg/kg和165.3±5.1 mg/kg。因此，成功地将四重同位素稀释（ID4）策略与DSPE-GC-MS方法相结合，提高了所建立的DSPE-GC-MS方法的准确度和精密度。

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引用次数: 0

Recent advances regarding development of effervescence reaction-assisted microextraction techniques for determination of organic pollutants in complex media 开发用于测定复杂介质中有机污染物的逸出反应辅助微萃取技术的最新进展

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-23 DOI: 10.1016/j.microc.2024.112249

Tingting Liu , Mizero Gloria , Xiaofeng Hou , Siyu Shen , Wondimu Andualem Ayele , Hongbing Qin , Qinqin Ma , Hailong Gong , Xuedong Wang

The effervescence reaction-assisted microextraction (EAM) technology can enhance the efficiency of dispersion technology. The in-situ generation of CO₂ resulting from the reaction between a CO₂ donor and a proton donor forms the basis for the EAM process, effectively dispersing the extractant phase into the sample through the gas produced. After being developed in 2011, EAM technology quickly gained much popularity. Numerous studies have employed it to improve pretreatment procedures. Due to its practical application, low operational cost, reduced solvent usage, excellent extraction efficiency, and environmental friendliness, the EAM technique has garnered significant attention. This article provides a comprehensive overview of the fundamental principles, influencing factors, and methodologies involved in the development of EAM technology in conjunction with large-scale instruments. It specifically focuses on the construction of diverse extractant-based methods for detecting organic pollutants in complex matrices such as environmental samples, food products, and biological specimens. The advantages and disadvantages of these methods are discussed in relation to their practical applications. Furthermore, this review elucidates the effective utilization of this approach for enriching and detecting pollutants within intricate matrices. By conducting a systematic analysis of recent advancements and research cases, this review serves as a valuable reference for advancing environmentally friendly, rapid, and convenient sample pretreatment techniques.

激发反应辅助微萃取（EAM）技术可提高分散技术的效率。二氧化碳供体和质子供体反应产生的二氧化碳是EAM工艺的基础，通过产生的气体将萃取剂相有效地分散到样品中。EAM 技术于 2011 年开发成功，并迅速得到广泛应用。许多研究都采用它来改进预处理程序。由于其实用性强、操作成本低、溶剂用量少、萃取效率高、环境友好等特点，EAM技术受到了广泛关注。本文全面概述了结合大型仪器开发 EAM 技术所涉及的基本原理、影响因素和方法。文章特别关注了基于萃取剂的多种方法的构建，以检测复杂基质（如环境样品、食品和生物标本）中的有机污染物。结合实际应用，讨论了这些方法的优缺点。此外，本综述还阐明了如何有效利用这种方法来富集和检测复杂基质中的污染物。通过对最新进展和研究案例进行系统分析，本综述对推进环保、快速、便捷的样品预处理技术具有重要参考价值。

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引用次数: 0

Deciphering versatile electrode materials in the electrochemical progressive processes for flutamide detection: A systematic review 解密氟他胺检测电化学递进过程中的多功能电极材料：系统综述

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-22 DOI: 10.1016/j.microc.2024.112244

Chellapandi Bhuvaneswari , Karuppaiya Palpandi , Boopathi Shagunthala Hariprasad , Chettipalayam Arunasalam Dhayanithi , Natarajan Raman , Periakaruppan Prakash , Sundaram Ganesh Babu

Excess of flutamide causes diseases, therefore excess of anything is undesirable. In order to safeguard human health and the environment, the scientific community battled to monitor hazardous and deadly substances, bacteria, gases, and radiation. So, we focus on the detection of Flutamide (FLT), a non-steroidal anti-androgen drug that is widely used in the treatment of prostatic carcinoma are rapidly attracted interest in the field of medical sciences because it is cherished in monitoring health and environmental conditions and their absurd negative effects when used in excess. This review article focuses on the important advancement in developing mixed and clinical nano samples-based on electrochemical sensors for simultaneous electrochemical sensing of FLT. The fabrication of different electrodes such as Diamond Electrode, Screen Printed Carbon Electrodes (SPCE), Glassy Carbon Electrode (GCE), Gold Electrode (AuE), Carbon Paste Electrode (CPE), and Graphite Pencil Lead Electrode used to detect FLT are highlighted. Furthermore, the challenges of translating different kinds of electrodes with supported nanomaterials-based sensors from the laboratory to the market are considered in future perspectives. This review discusses the selective reports on the different kinds of electrodes to discuss the application of FLT sensors during the period from 2012 to 2024. This review will be a useful tool for researchers, providing new perspectives and ideas for exciting new directions to explore.

过量的氟他胺会导致疾病，因此任何东西过量都是不可取的。为了保护人类健康和环境，科学界一直致力于监测有害和致命物质、细菌、气体和辐射。氟他胺（FLT）是一种非甾体抗雄激素药物，被广泛用于治疗前列腺癌，因其在监测健康和环境状况及其过量使用时产生的荒谬的负面影响而迅速引起了医学科学领域的兴趣。这篇综述文章重点介绍了在开发基于混合和临床纳米样品的电化学传感器以同时电化学检测 FLT 方面取得的重要进展。文章重点介绍了用于检测 FLT 的不同电极的制造方法，如金刚石电极、丝网印刷碳电极 (SPCE)、玻璃碳电极 (GCE)、金电极 (AuE)、碳浆电极 (CPE) 和石墨铅笔电极。此外，还从未来的角度探讨了将基于纳米材料的不同类型电极传感器从实验室推向市场所面临的挑战。本综述讨论了有关不同类型电极的选择性报告，以探讨 2012 年至 2024 年期间 FLT 传感器的应用情况。本综述将成为研究人员的有用工具，为探索激动人心的新方向提供新视角和新思路。

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引用次数: 0

Development of method for SARS-CoV-2 spike protein determination in saliva samples at fg mL−1 levels using electrochemically controlled preconcentration and molecularly imprinted polypyrrole sensor 用电化学控制预富集和分子印迹聚吡咯传感器测定唾液样品中fg mL - 1水平的SARS-CoV-2刺突蛋白方法的建立

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-22 DOI: 10.1016/j.microc.2024.112236

Milena do Prado Ferreira , Felipe Augusto Gorla , Mariana Gava Segatelli , Sueli Fumie Yamada Ogatta , Eduardo Costa Figueiredo , César Ricardo Teixeira Tarley

The present study describes a novel approach for designing a highly sensitive and selective electrochemical sensor for the detection of SARS-CoV-2 in saliva samples by sensing the S-protein, using an electrochemically controlled preconcentration. The sensor is based on electropolymerized molecularly imprinted polypyrrole (PPy) onto a screen-printed carbon electrode (MIP/PPy). The morphology of the MIP/PPy sensor was evaluated by SEM and AFM. The selectivity of the MIP/PPy compared to NIP/PPy was assessed by rebinding S-protein, followed by the monitoring of the redox probe ([Fe(CN)₆]^3−/4−). A substantial decrease in the anodic peak current of the redox probe was noticed when using the MIP/PPy. The detectability of the sensor was greatly improved (c.a 1,000,000 fold) by using controlled electrochemical preconcentration of S-protein at +0.6 V for 1.0 min in the rebinding process. Under this condition, a low limit of detection of 6.8fg mL⁻¹ and an analytical curve in the range of 50.0–125.0 fg mL⁻¹ (R² = 0.996) was obtained. The reproducibility for 10 authentic sensors was highly satisfactory with a relative standard deviation (%,RSD) of 2.8 %. Upon preparation, the sensor can be stored for up to 14 days under refrigeration, with a response efficiency greater than 85 %. The MIP/PPy exhibited high selectivity toward S-protein compared to antitrypsin, human serum albumin (HAS), transferrin, HIgG, and lysozyme. The sensor was applied for detecting SARS-CoV-2 virus through S-protein in infected patients’ saliva samples and validated by RT-PCR. In addition, the satisfactory recovery (91 to 103 %) in S-protein spiked negative samples attested to the free-interference analysis.

本研究描述了一种新的方法，设计了一种高灵敏度和选择性的电化学传感器，通过电化学控制的预浓缩，通过传感s蛋白来检测唾液样本中的SARS-CoV-2。该传感器基于电聚合分子印迹聚吡咯（PPy）到丝网印刷碳电极（MIP/PPy）上。利用扫描电镜和原子力显微镜对MIP/PPy传感器的形貌进行了表征。与NIP/PPy相比，MIP/PPy的选择性通过重新结合s蛋白来评估，然后监测氧化还原探针（[Fe(CN)6]3−/4−）。当使用MIP/PPy时，氧化还原探针的阳极峰值电流显着降低。在s蛋白重结合过程中，在+0.6 V下控制s蛋白的电化学预富集1.0 min，传感器的检测能力大大提高（约100万倍）。在此条件下，检测下限为6.8fg mL−1，分析曲线在50.0 ~ 125.0 fg mL−1范围内（R2 = 0.996）。10个真实传感器的重现性非常令人满意，相对标准偏差（%，RSD）为2.8%。制备完成后，传感器可在冷藏条件下保存长达14天，响应效率大于85%。与抗胰蛋白酶、人血清白蛋白（HAS）、转铁蛋白、HIgG和溶菌酶相比，MIP/PPy对s蛋白具有较高的选择性。将该传感器应用于感染患者唾液样本中s蛋白检测SARS-CoV-2病毒，并进行RT-PCR验证。此外，s蛋白加标阴性样品的回收率（91% ~ 103%）令人满意，证明了无干扰分析的有效性。

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引用次数: 0

Nuclear magnetic resonance (NMR) applications in biodiesel characterization and quality – A review 核磁共振 (NMR) 在生物柴油表征和质量中的应用 - 综述

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-19 DOI: 10.1016/j.microc.2024.112226

Alisson Geraldo Alves Ferreira, William Xavier Paula Lima, Rafael Cardoso Rial

This manuscript highlights the role of nuclear magnetic resonance (NMR) in assessing the quality and purity of biodiesel. NMR has proven to be an indispensable tool for biodiesel characterization, allowing for precise identification of key physicochemical properties such as methyl ester content, oxidative stability, free fatty acids, and glycerin residues. The ability of 1H NMR to detect impurities, adulterants, and structural changes during production and storage ensures compliance with international standards like ASTM D6751 and EN 14214, which are essential for maintaining biodiesel quality and performance. The study comprehensively compares the application of NMR in analyzing biodiesel from various feedstocks, including vegetable oils, animal fats, and waste oils. NMR’s capacity to discriminate biodiesel sources through specific chemical shifts, such as the methoxy group at 3.6 ppm, and identify contaminants, like water and residual catalysts, highlights its superiority over other analytical techniques like gas chromatography (GC) and Fourier-transform infrared spectroscopy (FTIR). Additionally, the manuscript underscores the importance of NMR in optimizing production processes by evaluating conversion efficiency and identifying by-products. The key contributions of this study lie in its detailed comparison of biodiesel from different feedstocks and its focus on the role of impurities and additives in affecting biodiesel quality. This work confirms NMR’s effectiveness as a reliable, non-destructive tool for ensuring biodiesel purity, making it an essential technique in advancing biodiesel production, improving process efficiency, and promoting the use of sustainable biofuels.

本手稿强调了核磁共振 (NMR) 在评估生物柴油质量和纯度方面的作用。事实证明，核磁共振是生物柴油表征不可或缺的工具，可精确鉴定关键理化性质，如甲酯含量、氧化稳定性、游离脂肪酸和甘油残留。1H NMR 能够检测生产和储存过程中的杂质、掺杂物和结构变化，确保符合 ASTM D6751 和 EN 14214 等国际标准，这对保持生物柴油的质量和性能至关重要。该研究全面比较了核磁共振在分析来自各种原料（包括植物油、动物脂肪和废油）的生物柴油中的应用。核磁共振能够通过特定的化学位移（如 3.6 ppm 的甲氧基）来鉴别生物柴油的来源，并识别污染物（如水和残留催化剂），这凸显了核磁共振比气相色谱法 (GC) 和傅立叶变换红外光谱法 (FTIR) 等其他分析技术的优越性。此外，手稿还强调了核磁共振在通过评估转化效率和识别副产品来优化生产过程方面的重要性。本研究的主要贡献在于详细比较了来自不同原料的生物柴油，并重点研究了杂质和添加剂对生物柴油质量的影响。这项工作证实了核磁共振作为确保生物柴油纯度的可靠、非破坏性工具的有效性，使其成为促进生物柴油生产、提高工艺效率和推广使用可持续生物燃料的重要技术。

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引用次数: 0

Nanosorbents in solid-phase extraction techniques for bioanalysis: A review 用于生物分析的固相萃取技术中的纳米吸附剂：综述

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-15 DOI: 10.1016/j.microc.2024.112170

Noorfatimah Yahaya , Nur Nadhirah Mohamad Zain , Ahmad Husaini Mohamed , Grasianto , Sazlinda Kamaruzaman , Mazidatulakmam Miskam , Rajeev Jain , Muggundha Raoov , Wan Nazwanie Wan Abdullah

In this article, we provide an in-depth review of the application of nanosorbents for bioanalytical sample preparation. Based on the reported literature, researchers are increasingly interested in using nanomaterials to enhance conventional sorptive extraction methods and develop innovative techniques in bioanalysis. The properties, advantages, and disadvantages of various classes of nanosorbents used in bioanalytical sample preparation were discussed in this study. Detailed mechanisms of separation, green metrics evaluation, and the challenges and potential of nanosorbents in solid-phase extraction techniques are also elaborated. It is hoped that this review will assist researchers in the development of ideal techniques for selective, fast, green, simple, sensitive, and accurate analytical methodologies for bioanalysis.

本文深入评述了纳米吸附剂在生物分析样品制备中的应用。根据文献报道，研究人员对使用纳米材料增强传统吸附萃取方法和开发生物分析创新技术的兴趣与日俱增。本研究讨论了用于生物分析样品制备的各类纳米吸附剂的特性、优缺点。此外，还详细阐述了纳米吸附剂在固相萃取技术中的分离机制、绿色指标评估、挑战和潜力。希望本综述能帮助研究人员开发出理想的技术，用于生物分析的选择性、快速、绿色、简单、灵敏和准确的分析方法。

引用次数: 0

Electrochemical methods for the detection of heavy metal ions: From sensors to biosensors 检测重金属离子的电化学方法：从传感器到生物传感器

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-13 DOI: 10.1016/j.microc.2024.112086

Hany Abd El-Raheem , Rabiaa Helim , Rabeay Y.A. Hassan , Ahmed F.A. Youssef , H. Korri-Youssoufi , Charoenkwan Kraiya

Naturally occurring heavy metals (HMs) present a serious risk to human health and the environment because of their toxicity, persistence, and tendency to bioaccumulate. Their presence in air, water, soil, and food can lead to a multitude of adverse effects, from acute poisoning to chronic health problems. The rapid expansion of industrialization and technology has increased HMs contamination from industrial emissions, mining, agriculture, urbanization, and e-waste, posing serious risks to ecosystems and public health. To tackle this increasing challenge, it is essential to develop highly robust, sensitive, and selective electrochemical detection methods for the rapid identification of heavy metal ions (HMIs) contaminants. This review investigates a variety of electrochemical sensors and biosensors utilizing voltammetric techniques for HMIs detection. It covers voltammetric methods such as cyclic voltammetry (CV), square wave voltammetry (SWV), differential-pulse voltammetry (DPV), normal pulse voltammetry (NPV), staircase voltammetry (SV), linear sweep voltammetry (LSV), hydrodynamic voltammetry (HV), anodic stripping voltammetry (ASV), cathodic stripping voltammetry (CSV), and adsorptive stripping voltammetry (AdSV). The review also emphasizes recent advancements in the electrochemical detection of HMIs through various sensor and biosensor platforms, including different nanomaterials and DNA aptamers. These innovations have potential applications in environmental monitoring, food and water safety, industrial waste management, and public health protection, making them vital tools for safeguarding human health and the environment from HMIs contamination.

天然重金属（HMs）因其毒性、持久性和生物累积倾向，对人类健康和环境构成严重威胁。它们在空气、水、土壤和食物中的存在会导致从急性中毒到慢性健康问题等多种不良影响。工业化和技术的快速发展加剧了工业排放、采矿、农业、城市化和电子垃圾等造成的 HMs 污染，对生态系统和公众健康构成了严重威胁。为应对这一日益严峻的挑战，必须开发高度可靠、灵敏和选择性强的电化学检测方法，以快速识别重金属离子（HMIs）污染物。本综述研究了利用伏安技术检测重金属离子的各种电化学传感器和生物传感器。它涵盖了各种伏安方法，如循环伏安法（CV）、方波伏安法（SWV）、差分脉冲伏安法（DPV）、正常脉冲伏安法（NPV）、阶梯伏安法（SV）、线性扫描伏安法 (LSV)、流体动力伏安法 (HV)、阳极剥离伏安法 (ASV)、阴极剥离伏安法 (CSV) 和吸附剥离伏安法 (AdSV)。综述还强调了通过各种传感器和生物传感器平台（包括不同的纳米材料和 DNA 类似物）对 HMIs 进行电化学检测的最新进展。这些创新技术在环境监测、食品和水安全、工业废物管理和公共卫生保护方面具有潜在的应用前景，是保护人类健康和环境免受 HMIs 污染的重要工具。

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引用次数: 0

Recent advances in zearalenone clean-up approaches from polluted food and feed: A mini-review of the State-of-the-art developments 受污染食品和饲料中玉米赤霉烯酮清除方法的最新进展：最新进展小回顾

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-11-13 DOI: 10.1016/j.microc.2024.112182

M.A. Diab , Heba A. El-Sabban , Kwang-Hyun Baek

Zearalenone (ZEN), a mycotoxin generated by fusarium species, is often found in food and feed and can be extremely harmful to human and animal health. Warranting food safety requires effective extraction of ZEN from contaminated matrices. This mini-review concisely summarizes the most recent ZEN extraction techniques from contaminated food and feed. It highlights firstly various extraction techniques, including liquid–liquid extraction (LLE), solid phase extraction using molecularly imprinted polymers (SPE-MIPs), [Quick, Easy, Cheap, Effective, Rugged, and Safe method (QUEChERS)], green extraction methods such as ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) to improve extraction approaches. Secondly, it discusses recent advanced methods of ZEN detection. This review also discusses the challenges associated with ZEN extraction such as matrix effects, co-contaminants, and the need for standardization method. It emphasizes the importance of continued research to refine existing techniques and develop novel strategies that ensure high efficiency, specificity, and environmental sustainability.

玉米赤霉烯酮（ZEN）是一种由镰刀菌产生的霉菌毒素，经常出现在食品和饲料中，对人类和动物的健康极为有害。要保证食品安全，就必须从受污染的基质中有效提取玉米赤霉烯酮。本微型综述简明扼要地总结了从受污染的食品和饲料中提取 ZEN 的最新技术。首先，它重点介绍了各种萃取技术，包括液-液萃取（LLE）、使用分子印迹聚合物的固相萃取（SPE-MIPs）、[快速、简便、廉价、有效、坚固和安全的方法（QUEChERS）]、绿色萃取方法（如超声辅助萃取（UAE）和微波辅助萃取（MAE）），以改进萃取方法。其次，它还讨论了最新的 ZEN 检测先进方法。本综述还讨论了与锌萃取相关的挑战，如基质效应、共污染物以及标准化方法的必要性。综述强调了继续开展研究以完善现有技术并开发新型策略的重要性，从而确保高效率、特异性和环境可持续性。

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