Microchemical Journal最新文献

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Ti–O coupled graphene edges induced high electrochemical activity of the carbon film

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-04 DOI: 10.1016/j.microc.2025.113258

Yuanyuan Cao, Linwang Li, Xuebin Liu, Haohua Zhong

In this work, we investigated the electrochemical activity induced by the Ti–O coupled graphene edges on the graphene structured carbon (GSC) film. The Ti-O-GSC film was prepared by the electron cyclotron resonance (ECR) plasma sputtering system under low energy electron irradiation with O and Ti atoms doped in one step. According to the nanostructure characterization, the graphene edges and groups with O and Ti were formed on the surface of film. In the Fe(CN)₆^4−/3− and Fe^3+/2+ redox systems, the redox peak separation was reduced to 69.6 m V and 611.5 m V, respectively, and the charge-transfer resistance decreased from 507.50 Ω·cm² to 15.88 Ω·cm², due to the doping of O and Ti. In the sensing of the DNA bases, the Ti-O-GSC film reduced the oxidation potentials to 138 mV and 755.2 mV for adenine and guanine, and realized the simultaneous detection of adenine and guanine with detection limits of 1.09 μM and 0.76 μM. The mechanism could be that the simultaneous presence of Ti and O atoms at the graphene edge facilitates numerous active sites, improving the electrochemical activity of the Ti-O-GSC film. These results indicate that the Ti-O-GSC films are promising electrode materials to construct sensitive electrochemical biosensors.

{"title":"Ti–O coupled graphene edges induced high electrochemical activity of the carbon film","authors":"Yuanyuan Cao, Linwang Li, Xuebin Liu, Haohua Zhong","doi":"10.1016/j.microc.2025.113258","DOIUrl":"10.1016/j.microc.2025.113258","url":null,"abstract":"<div><div>In this work, we investigated the electrochemical activity induced by the Ti–O coupled graphene edges on the graphene structured carbon (GSC) film. The Ti-O-GSC film was prepared by the electron cyclotron resonance (ECR) plasma sputtering system under low energy electron irradiation with O and Ti atoms doped in one step. According to the nanostructure characterization, the graphene edges and groups with O and Ti were formed on the surface of film. In the Fe(CN)<sub>6</sub><sup>4−/3−</sup> and Fe<sup>3+/2+</sup> redox systems, the redox peak separation was reduced to 69.6 m V and 611.5 m V, respectively, and the charge-transfer resistance decreased from 507.50 Ω·cm<sup>2</sup> to 15.88 Ω·cm<sup>2</sup>, due to the doping of O and Ti. In the sensing of the DNA bases, the Ti-O-GSC film reduced the oxidation potentials to 138 mV and 755.2 mV for adenine and guanine, and realized the simultaneous detection of adenine and guanine with detection limits of 1.09 μM and 0.76 μM. The mechanism could be that the simultaneous presence of Ti and O atoms at the graphene edge facilitates numerous active sites, improving the electrochemical activity of the Ti-O-GSC film. These results indicate that the Ti-O-GSC films are promising electrode materials to construct sensitive electrochemical biosensors.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113258"},"PeriodicalIF":4.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive molecularly imprinted poly(o-aminophenol)-based electrochemical sensor for propiconazole in food samples using reduced graphene oxide- chitosan composite

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-04 DOI: 10.1016/j.microc.2025.113255

Reda Elshafey, Ghada Fathey Abo–Sobehy, Abd–Elgawad Radi

The presence of propiconazole (PRO), an endocrine disruptor and persistent triazole fungicide, in surface water, demanded the development of simple, and sensitive sensors. A reusable molecularly imprinted polymer (MIP)-based electrochemical sensor was developed for the sensitive detection of PRO using poly (o-aminophenol) and an electrochemically reduced graphene oxide-chitosan (ERGO-CS). The poly o-aminophenol recognition receptors were electrogenerated on the surface of an ERGO-modified glassy carbon electrode in the presence of PRO as a template. The ERGO-CS film improved the electrode surface area which loads a high density of PRO-imprinted sites on the electrode surface. Scanning electron microscopy, voltammetric, and impedance techniques demonstrated the successful fabrication of the MIP sensor. The drop in the peak current and increment in the charge transfer resistance of the redox probe [Fe(CN)₆]^3−/4− upon selective recognition of RPO to the MIP sensor are employed as the sensor responses.

Under the optimal parameters of monomer type, monomer to target molar ratio, electropolymerization conditions, and the removal method, the MIP sensor achieved two linear responses over the PRO concentration range of (0.01 pM to 1 pM) and (1 pM to 10 µM) with a limit of detection of about 0.008 pM for DPV and EIS methods. The MIP sensor exhibited superior selectivity against the interferent pesticides. The PRO-MIP sensor exhibited acceptable reproducibility and can be regenerated up to 6 cycles. The MIP sensor has shown satisfactory accuracy for the quantification of PRO in soil, agriculture water, wheat flour, milk, and cucumber samples with good recovery 87.59 % to 117.94 %) and RSD of 1.79 to 14.98 %. The MIP sensor offers a promising alternative for monitoring PRO in environmental samples.

{"title":"A sensitive molecularly imprinted poly(o-aminophenol)-based electrochemical sensor for propiconazole in food samples using reduced graphene oxide- chitosan composite","authors":"Reda Elshafey, Ghada Fathey Abo–Sobehy, Abd–Elgawad Radi","doi":"10.1016/j.microc.2025.113255","DOIUrl":"10.1016/j.microc.2025.113255","url":null,"abstract":"<div><div>The presence of propiconazole (PRO), an endocrine disruptor and persistent triazole fungicide, in surface water, demanded the development of simple, and sensitive sensors. A reusable molecularly imprinted polymer (MIP)-based electrochemical sensor was developed for the sensitive detection of PRO using poly (<em>o</em>-aminophenol) and an electrochemically reduced graphene oxide-chitosan (ERGO-CS). The poly <em>o</em>-aminophenol recognition receptors were electrogenerated on the surface of an ERGO-modified glassy carbon electrode in the presence of PRO as a template. The ERGO-CS film improved the electrode surface area which loads a high density of PRO-imprinted sites on the electrode surface. Scanning electron microscopy, voltammetric, and impedance techniques demonstrated the successful fabrication of the MIP sensor. The drop in the peak current and increment in the charge transfer resistance of the redox probe [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup> upon selective recognition of RPO to the MIP sensor are employed as the sensor responses.</div><div>Under the optimal parameters of monomer type, monomer to target molar ratio, electropolymerization conditions, and the removal method, the MIP sensor achieved two linear responses over the PRO concentration range of (0.01 pM to 1 pM) and (1 pM to 10 µM) with a limit of detection of about 0.008 pM for DPV and EIS methods. The MIP sensor exhibited superior selectivity against the interferent pesticides. The PRO-MIP sensor exhibited acceptable reproducibility and can be regenerated up to 6 cycles. The MIP sensor has shown satisfactory accuracy for the quantification of PRO in soil, agriculture water, wheat flour, milk, and cucumber samples with good recovery 87.59 % to 117.94 %) and RSD of 1.79 to 14.98 %. The MIP sensor offers a promising alternative for monitoring PRO in environmental samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113255"},"PeriodicalIF":4.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Classification and research progress of aggregation-induced electrochemiluminescence materials for sensoring application

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-04 DOI: 10.1016/j.microc.2025.113241

Di Zhang , Chenchen Li , Tai Xiang , Yanbao Yu , Rui Xu , Yong Zhang

In recent years, the rapid development of electrochemiluminescence (ECL) sensors has driven the design of novel luminophores to meet the demands for high sensitivity and reliability in analytical applications. Since Cola introduced the concept of aggregation-induced electrochemiluminescence (AIECL), AIECL materials have opened new avenues for optimizing ECL sensors. This paper focuses on AIECL materials, classifies them based on their structures, and summarizes their applications in ECL sensors. Finally, inspired by the use of AIECL luminophores, the prospects and challenges of AIECL materials and sensors are discussed, highlighting their significance in advancing sensing and analytical technologies.

引用次数: 0

Multimodal hybrid linear auto-weighting models: Application of ultraviolet spectroscopy for growth prediction of marine pathogenic bacteria

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-03 DOI: 10.1016/j.microc.2025.113209

Ying Chen, Jin Wang, Junfei Liu, Junru Zhang, Chenglong Wang, Wanwen Li

Spectral contactless detection techniques are real-time and extremely sensitive. It shows great potential for rapid prediction of growth trends of marine microorganisms. This paper proposes a real-time detection system that integrates ultraviolet spectroscopy (UV) technology with the PPSA model (1DCNN-PLSR Parallel 1DCNN-SVR Adaptation). The system realized the detection of microbial concentration and the prediction of growth trend. A UV absorption spectral dataset was constructed by collecting ultraviolet (UV) spectral data of actinomycetes during growth and combining it with the microscopic hemocyte plate counting method. For multiple feature intervals characterizing actinomycetes in UV absorption spectral curves, spectral data feature extraction was performed by a one-dimensional convolutional neural network (1DCNN), and prediction experiments were conducted using multiple parallel network models. Further exploration of weight adaptive fine-tuning of model parameter shares to optimize overall prediction accuracy. The experimental evaluation showed that the model achieved an R² of 0.9994, an MAE of 0.4181, and an average error of 2.0 * 10⁶cells/mL in the assay results. Rapid characterization and detection of the growth of known and unknown pathogenic bacteria using ultraviolet absorption spectroscopy is valuable for the study of marine and human ecosystems.

{"title":"Multimodal hybrid linear auto-weighting models: Application of ultraviolet spectroscopy for growth prediction of marine pathogenic bacteria","authors":"Ying Chen, Jin Wang, Junfei Liu, Junru Zhang, Chenglong Wang, Wanwen Li","doi":"10.1016/j.microc.2025.113209","DOIUrl":"10.1016/j.microc.2025.113209","url":null,"abstract":"<div><div>Spectral contactless detection techniques are real-time and extremely sensitive. It shows great potential for rapid prediction of growth trends of marine microorganisms. This paper proposes a real-time detection system that integrates ultraviolet spectroscopy (UV) technology with the PPSA model (1DCNN-PLSR Parallel 1DCNN-SVR Adaptation). The system realized the detection of microbial concentration and the prediction of growth trend. A UV absorption spectral dataset was constructed by collecting ultraviolet (UV) spectral data of actinomycetes during growth and combining it with the microscopic hemocyte plate counting method. For multiple feature intervals characterizing actinomycetes in UV absorption spectral curves, spectral data feature extraction was performed by a one-dimensional convolutional neural network (1DCNN), and prediction experiments were conducted using multiple parallel network models. Further exploration of weight adaptive fine-tuning of model parameter shares to optimize overall prediction accuracy. The experimental evaluation showed that the model achieved an R<sup>2</sup> of 0.9994, an MAE of 0.4181, and an average error of 2.0 * 10<sup>6</sup>cells/mL in the assay results. Rapid characterization and detection of the growth of known and unknown pathogenic bacteria using ultraviolet absorption spectroscopy is valuable for the study of marine and human ecosystems.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113209"},"PeriodicalIF":4.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen dioxide monitoring using alternative sorbent-based cotton fabric with smartphone-integrated colorimetric detection

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-03 DOI: 10.1016/j.microc.2025.113231

Sutasinee Apichai , Thanawat Pattananandecha , Kanokwan Kiwfo , Kate Grudpan , Wolfgang Frenzel , Chalermpong Saenjum

A cost-effective and sustainable approach for monitoring the concentration of nitrogen dioxide (NO₂) in air was developed using smartphone-based colorimetric detection and passive air sampling. The cotton-based adsorbent moistened with triethanolamine was assembled into a passive sampler to sample NO₂ in air. NO₂ was then converted to nitrite which reacted with the Griess reagent on the cotton pads to produce a pink product. The product was photographed in a light-controlled box and subsequently analyzed using a custom-built app to convert it to NO₂ concentration. The detection and quantification limits were 0.07 and 0.21 µg, respectively. Field deployment of the developed approach for 48 and 96 h in high-traffic areas demonstrated NO₂ measurements statistically comparable with that of spectrophotometric analysis at a 95 % confidence level. In addition, the on-site monitoring of NO₂ by the developed approach was also agreed with active sampling in connection with a chemiluminescence-based NO₂ analyzer. The developed approach demonstrated effective ambient NO₂ monitoring in high-traffic areas along Suthep Road, Muang, Chiang Mai, Thailand, with continuous measurements over three days. This approach enabled a cost-effective acquisition of more spatially and temporally representative air pollution data. It aligns with the UN Sustainable Development Goal 3 by helping to reduce health risks from hazardous chemicals and environmental pollution.

{"title":"Nitrogen dioxide monitoring using alternative sorbent-based cotton fabric with smartphone-integrated colorimetric detection","authors":"Sutasinee Apichai , Thanawat Pattananandecha , Kanokwan Kiwfo , Kate Grudpan , Wolfgang Frenzel , Chalermpong Saenjum","doi":"10.1016/j.microc.2025.113231","DOIUrl":"10.1016/j.microc.2025.113231","url":null,"abstract":"<div><div>A cost-effective and sustainable approach for monitoring the concentration of nitrogen dioxide (NO<sub>2</sub>) in air was developed using smartphone-based colorimetric detection and passive air sampling. The cotton-based adsorbent moistened with triethanolamine was assembled into a passive sampler to sample NO<sub>2</sub> in air. NO<sub>2</sub> was then converted to nitrite which reacted with the Griess reagent on the cotton pads to produce a pink product. The product was photographed in a light-controlled box and subsequently analyzed using a custom-built app to convert it to NO<sub>2</sub> concentration. The detection and quantification limits were 0.07 and 0.21 µg, respectively. Field deployment of the developed approach for 48 and 96 h in high-traffic areas demonstrated NO<sub>2</sub> measurements statistically comparable with that of spectrophotometric analysis at a 95 % confidence level. In addition, the on-site monitoring of NO<sub>2</sub> by the developed approach was also agreed with active sampling in connection with a chemiluminescence-based NO<sub>2</sub> analyzer. The developed approach demonstrated effective ambient NO<sub>2</sub> monitoring in high-traffic areas along Suthep Road, Muang, Chiang Mai, Thailand, with continuous measurements over three days. This approach enabled a cost-effective acquisition of more spatially and temporally representative air pollution data. It aligns with the UN Sustainable Development Goal 3 by helping to reduce health risks from hazardous chemicals and environmental pollution.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113231"},"PeriodicalIF":4.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroanalysis of phenylalanine in food supplement by differential pulse voltammetry technique: Application of economical and handmade Sonogel-Carbon electrodes improved with a fast polarization step

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113208

Alfonso Sierra-Padilla , Alessandro Monari , José María Palacios-Santander , Juan José García-Guzmán , Laura Pigani , Laura Cubillana-Aguilera

Sonogel-Carbon electrodes have been applied successfully in the determination of phenylalanine (Phe). These handmade economical sensor devices exhibit better electroanalytical performance than commercial electrodes, and by a fast surface polarization step the electrochemical signal was significantly increased. Improvements in the electrochemically active surface area and electrical conductivity were demonstrated. Excellent figures of merits were assessed, such as a sensitivity value of 68.19 ± 3.11 µA mM⁻¹ cm⁻² comparable to other previously reported devices and limit of detection of 31.92 ± 3.75 µM. Sonogel-Carbon electrodes exhibit worthy selectivity in the presence of other amino acids and electroactive species. Finally, satisfactory recovery values between 96 and 101 % were obtained when this material was applied in the determination of Phe in a food supplement sample. Sonogel-Carbon electrodes stand out compared to other reported sensor devices due to their simple, low-cost and eco-friendly manufacturing; their reusability; and their robust and selective electrochemical performance.

{"title":"Electroanalysis of phenylalanine in food supplement by differential pulse voltammetry technique: Application of economical and handmade Sonogel-Carbon electrodes improved with a fast polarization step","authors":"Alfonso Sierra-Padilla , Alessandro Monari , José María Palacios-Santander , Juan José García-Guzmán , Laura Pigani , Laura Cubillana-Aguilera","doi":"10.1016/j.microc.2025.113208","DOIUrl":"10.1016/j.microc.2025.113208","url":null,"abstract":"<div><div>Sonogel-Carbon electrodes have been applied successfully in the determination of phenylalanine (Phe). These handmade economical sensor devices exhibit better electroanalytical performance than commercial electrodes, and by a fast surface polarization step the electrochemical signal was significantly increased. Improvements in the electrochemically active surface area and electrical conductivity were demonstrated. Excellent figures of merits were assessed, such as a sensitivity value of 68.19 ± 3.11 µA mM<sup>−1</sup> cm<sup>−2</sup> comparable to other previously reported devices and limit of detection of 31.92 ± 3.75 µM. Sonogel-Carbon electrodes exhibit worthy selectivity in the presence of other amino acids and electroactive species. Finally, satisfactory recovery values between 96 and 101 % were obtained when this material was applied in the determination of Phe in a food supplement sample. Sonogel-Carbon electrodes stand out compared to other reported sensor devices due to their simple, low-cost and eco-friendly manufacturing; their reusability; and their robust and selective electrochemical performance.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113208"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adhesive-emitted odorants detection using an electronic nose: Unraveling algorithm applicability with controlled dataset characteristics

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113220

Chen Qu , Zhuoran Zhang , Ning Liu , Zixuan Zhao , Zhongbao Guo , Jinhua Liu , Jiemin Liu , Chuandong Wu

Electronic nose technology is becoming increasingly important in pollution monitoring; however, the inappropriate selection of pattern recognition algorithms may lead to performance decreases. In this study, an electronic nose was developed for the qualitative classification of adhesives and quantitative detection of adhesive-emitted odorant concentrations. Meanwhile, the applicability of commonly used pattern recognition algorithms (support vector regression, partial least squares regression, artificial neural network (ANN), random forest regression (RFR), ridge regression, and Lasso regression) to datasets with controlled volumes and interference intensities was investigated by comparing their quantitative performance. In qualitative analysis, the support vector machine with polynomial nonlinear kernel and random forest achieved 100% accurate classification of 11 adhesive samples. In quantitative analysis, RFR demonstrated good generalization ability and interference insensitivity, with a mean absolute percentage error (MAPE) below 3% in the large-volume strongly interfering dataset and less than 25% in the small-volume strongly interfering dataset. While ANN showed certain data volume dependence and interference sensitivity. For dataset with small volume and weak interference, algorithms with simple structures could achieve accurate quantification (MAPE of 10%). Moreover, the algorithm applicability was validated on homologous datasets and the effectiveness of cluster analysis to remove outliers was discussed.

{"title":"Adhesive-emitted odorants detection using an electronic nose: Unraveling algorithm applicability with controlled dataset characteristics","authors":"Chen Qu , Zhuoran Zhang , Ning Liu , Zixuan Zhao , Zhongbao Guo , Jinhua Liu , Jiemin Liu , Chuandong Wu","doi":"10.1016/j.microc.2025.113220","DOIUrl":"10.1016/j.microc.2025.113220","url":null,"abstract":"<div><div>Electronic nose technology is becoming increasingly important in pollution monitoring; however, the inappropriate selection of pattern recognition algorithms may lead to performance decreases. In this study, an electronic nose was developed for the qualitative classification of adhesives and quantitative detection of adhesive-emitted odorant concentrations. Meanwhile, the applicability of commonly used pattern recognition algorithms (support vector regression, partial least squares regression, artificial neural network (ANN), random forest regression (RFR), ridge regression, and Lasso regression) to datasets with controlled volumes and interference intensities was investigated by comparing their quantitative performance. In qualitative analysis, the support vector machine with polynomial nonlinear kernel and random forest achieved 100% accurate classification of 11 adhesive samples. In quantitative analysis, RFR demonstrated good generalization ability and interference insensitivity, with a mean absolute percentage error (MAPE) below 3% in the large-volume strongly interfering dataset and less than 25% in the small-volume strongly interfering dataset. While ANN showed certain data volume dependence and interference sensitivity. For dataset with small volume and weak interference, algorithms with simple structures could achieve accurate quantification (MAPE of 10%). Moreover, the algorithm applicability was validated on homologous datasets and the effectiveness of cluster analysis to remove outliers was discussed.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113220"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an origami paper-based electrochemical sensor using N-doped graphene for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113223

Jiaqi Wei , Linzhe Wang , Jingfang Hu , Wensong Wei , Yangchun Yang , Yu Song , Yansheng Li , Guowei Gao

Heavy metals expose great hazards to the ecological environment and public health due to their high toxicity and non-biodegradability. In this work, a low-cost, portable origami electrochemical microfluidic paper-based analytical device (EµPAD) was developed using nitrogen-doped graphene (NG) for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water. The origami structure functions as a valve between sample introduction and detection, reducing sample volume and enhancing operation convenience. Electrochemical linear scanning voltammetry was employed for the first time in the in-situ synthesis of NG on paper-based electrode for sensing heavy metal ions. With its large specific surface area and numerous active sites, NG-modified electrodes exhibited excellent analytical performance for simultaneous determination of Cd(II), Pb(II), and Hg(II). The EµPAD sensor exhibited a wide linear response ranging from 5 to 100 µg/L, and low detection limits (Cd(II): 0.5698 µg/L, Pb(II): 0.4024 µg/L, Hg(II): 0.2565 µg/L,), which were well below the drinking water standards. Additionally, the EµPAD has been successfully applied to real water samples, achieving recoveries of 96.4 % ∼106.2 % with RSDs below 7 %.

{"title":"Development of an origami paper-based electrochemical sensor using N-doped graphene for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water","authors":"Jiaqi Wei , Linzhe Wang , Jingfang Hu , Wensong Wei , Yangchun Yang , Yu Song , Yansheng Li , Guowei Gao","doi":"10.1016/j.microc.2025.113223","DOIUrl":"10.1016/j.microc.2025.113223","url":null,"abstract":"<div><div>Heavy metals expose great hazards to the ecological environment and public health due to their high toxicity and non-biodegradability. In this work, a low-cost, portable origami electrochemical microfluidic paper-based analytical device (EµPAD) was developed using nitrogen-doped graphene (NG) for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water. The origami structure functions as a valve between sample introduction and detection, reducing sample volume and enhancing operation convenience. Electrochemical linear scanning voltammetry was employed for the first time in the in-situ synthesis of NG on paper-based electrode for sensing heavy metal ions. With its large specific surface area and numerous active sites, NG-modified electrodes exhibited excellent analytical performance for simultaneous determination of Cd(II), Pb(II), and Hg(II). The EµPAD sensor exhibited a wide linear response ranging from 5 to 100 µg/L, and low detection limits (Cd(II): 0.5698 µg/L, Pb(II): 0.4024 µg/L, Hg(II): 0.2565 µg/L,), which were well below the drinking water standards. Additionally, the EµPAD has been successfully applied to real water samples, achieving recoveries of 96.4 % ∼106.2 % with RSDs below 7 %.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113223"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ratiometric fluorescent detection of heparan sulfate in human plasma and serum using peptide-based fluorescent probes

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113222

Sumita Subedi, Kishor Khadka, Myeong-geun Park, Inae Jeon, Moodong Cho, Keun-Hyeung Lee

The level of heparan sulfate (HS) in human blood acts as a biomarker for several diseases, making the development of sensitive and selective detection methods increasingly important. Until now, there has been no ratiometric fluorescent detection method for sensing HS in blood samples. In this study, we report a novel ratiometric fluorescent detection method for HS in blood samples using two peptide-based fluorescent probes: probe 1, containing six Arg residues, and probe 2, containing two Arg residues and four Lys residues. Both probes, excited with visible light at 430 nm, exhibited ratiometric fluorescence responses to HS in human plasma solutions. However, probe 1 exhibited a highly sensitive ratiometric response to HS at nanomolar concentrations (0–300 nM) in 50 % human plasma samples, whereas probe 2 exhibited ratiometric responses at nanomolar concentrations (0–800 nM) in plasma samples containing up to 30 % human plasma. Probe 1 also showed a highly selective ratiometric response to HS over heparin and other biological competitors in human plasma samples. The detection limit of probe 1 was determined to be 14.23 nM in 50 % human plasma samples. Structural analysis of the developed fluorescent probes revealed that the presence of six guanidine groups, rather than amino groups, significantly enhances HS detection in blood samples by minimizing non-specific binding to biomolecules. This work represents the first approach to quantifying HS levels in human serum and plasma samples using ratiometric fluorescence techniques, providing a promising tool for disease biomarker identification and clinical diagnostics.

{"title":"Ratiometric fluorescent detection of heparan sulfate in human plasma and serum using peptide-based fluorescent probes","authors":"Sumita Subedi, Kishor Khadka, Myeong-geun Park, Inae Jeon, Moodong Cho, Keun-Hyeung Lee","doi":"10.1016/j.microc.2025.113222","DOIUrl":"10.1016/j.microc.2025.113222","url":null,"abstract":"<div><div>The level of heparan sulfate (HS) in human blood acts as a biomarker for several diseases, making the development of sensitive and selective detection methods increasingly important. Until now, there has been no ratiometric fluorescent detection method for sensing HS in blood samples. In this study, we report a novel ratiometric fluorescent detection method for HS in blood samples using two peptide-based fluorescent probes: probe <strong>1</strong>, containing six Arg residues, and probe <strong>2</strong>, containing two Arg residues and four Lys residues. Both probes, excited with visible light at 430 nm, exhibited ratiometric fluorescence responses to HS in human plasma solutions. However, probe <strong>1</strong> exhibited a highly sensitive ratiometric response to HS at nanomolar concentrations (0–300 nM) in 50 % human plasma samples, whereas probe <strong>2</strong> exhibited ratiometric responses at nanomolar concentrations (0–800 nM) in plasma samples containing up to 30 % human plasma. Probe <strong>1</strong> also showed a highly selective ratiometric response to HS over heparin and other biological competitors in human plasma samples. The detection limit of probe <strong>1</strong> was determined to be 14.23 nM in 50 % human plasma samples. Structural analysis of the developed fluorescent probes revealed that the presence of six guanidine groups, rather than amino groups, significantly enhances HS detection in blood samples by minimizing non-specific binding to biomolecules. This work represents the first approach to quantifying HS levels in human serum and plasma samples using ratiometric fluorescence techniques, providing a promising tool for disease biomarker identification and clinical diagnostics.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113222"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyridine-substituted cyanostilbene macrocycle: A “turn-on” fluorescence sensor for pesticide bromoxynil octanoate

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113221

Pan He , Sining Zheng , Yun Li , Hongyu Guo , Fafu Yang

Pesticide bromoxynil octanoate is widely used in agriculture but possesses the potential toxicity to both animals and humans. The development of facile and on-site detection method for bromoxynil octanoate is extremely expected. In this work, the first example of fluorescent sensor for on-site examining bromoxynil octanoate in aqueous media was reported based on a pyridine-substituted cyanostilbene macrocycle (PSCM). This fluorescent sensor exhibited strong “turn-on” orange fluorescence on sensing bromoxynil octanoate with high selectivity and sensitivity among a wide range of ions and pesticides. The limitation of detection is 2.1 × 10⁻⁸ M. The study on binding mechanism suggested that bromoxynil octanoate is located in the cavity of PSCM based on π-π and hydrophobic interactions. The application experiments indicated that this fluorescent sensor has the excellent sensing behavior on test papers, four real agriculture samples and simulated water samples, implying the good application potential on facile, simple and on-site detecting bromoxynil octanoate in real environments.

{"title":"Pyridine-substituted cyanostilbene macrocycle: A “turn-on” fluorescence sensor for pesticide bromoxynil octanoate","authors":"Pan He , Sining Zheng , Yun Li , Hongyu Guo , Fafu Yang","doi":"10.1016/j.microc.2025.113221","DOIUrl":"10.1016/j.microc.2025.113221","url":null,"abstract":"<div><div>Pesticide bromoxynil octanoate is widely used in agriculture but possesses the potential toxicity to both animals and humans. The development of facile and on-site detection method for bromoxynil octanoate is extremely expected. In this work, the first example of fluorescent sensor for on-site examining bromoxynil octanoate in aqueous media was reported based on a pyridine-substituted cyanostilbene macrocycle (<strong>PSCM</strong>). This fluorescent sensor exhibited strong “turn-on” orange fluorescence on sensing bromoxynil octanoate with high selectivity and sensitivity among a wide range of ions and pesticides. The limitation of detection is 2.1 × 10<sup>−8</sup> M. The study on binding mechanism suggested that bromoxynil octanoate is located in the cavity of <strong>PSCM</strong> based on π-π and hydrophobic interactions. The application experiments indicated that this fluorescent sensor has the excellent sensing behavior on test papers, four real agriculture samples and simulated water samples, implying the good application potential on facile, simple and on-site detecting bromoxynil octanoate in real environments.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113221"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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