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Evaluation of Nitrate Reductase Assay For Rapid Detection of Drug Resistant Tuberculosis 硝酸还原酶法快速检测耐药结核病的评价
Q4 Medicine Pub Date : 2019-11-24 DOI: 10.37107/jhas.53
Ranjit Kumar Sah, Dwij Raj Bhatta, Gokarna Raj Ghimire, Bhuwaneswor Prasad Kandel, Bishnu Raj Tiwari, Jeevan Bahadur Sherchand
Emergence of multidrug-resistant tuberculosis (MDR-TB) urgently demands for simple, rapid and inexpensive methods of its detection for the effective treatment of drug resistant tuberculosis, particularly in low-income countries. This prospective study was carried out at National Tuberculosis Reference Laboratory and South Asian Association of Regional Cooperation (SAARC) Tuberculosis and HIV/AIDS Centre, Thimi, Bhaktapur, Nepal, from November 2009 to May 2010 to evaluate nitrate reductase assay (NRA) efficacy for rapid determination of streptomycin, isoniazid, rifampicin and ethambutol susceptibility of Mycobacterium tuberculosis strains. A total of 113 clinical isolates of M. tuberculosis were tested for four first line antitubercular drugs by nitrate reductase assay and were compared with standard proportion method. Results were available in 7-14 days by NRA as compared to proportion method which generally takes 4-6 weeks. The sensitivity and specificity of NRA were 98.1% and 100% for isoniazid, 95.1% and 98.6% for rifampicin, 91.4% and 94.9% for streptomycin, and 78.6% and 97.9% for ethambutol respectively. Agreement between NRA and proportion method were 99.1%, 97.3%, 93.8%, 95.6% for isoniazid, rifampicin, streptomycin and ethambutol, respectively. NRA is easier, inexpensive and reliable method for susceptibility testing of Mycobacterum tuberculosis for isoniazid and rifampicin, the two most important drugs for the treatment of tuberculosis. The reduction in susceptibility testing time, and higher sensitivity and specificity of NRA method is of fundamental importance in detecting MDR-TB. Key words: Drug susceptibility, MDR-TB, NRA, proportion method
耐多药结核病(MDR-TB)的出现迫切需要简单、快速和廉价的检测方法,以便有效治疗耐药结核病,特别是在低收入国家。本前瞻性研究于2009年11月至2010年5月在尼泊尔巴克塔普尔Thimi的国家结核病参考实验室和南亚区域合作联盟结核病和艾滋病毒/艾滋病中心开展,目的是评价硝酸还原酶测定法(NRA)快速测定结核分枝杆菌菌株链霉素、异烟肼、利福平和乙胺丁醇敏感性的有效性。采用硝酸还原酶法对113株临床分离的结核分枝杆菌进行4种一线抗结核药物的检测,并与标准比例法进行比较。与比例法一般需要4-6周的时间相比,NRA法在7-14天内可得到结果。NRA对异烟肼、利福平、链霉素、乙胺丁醇的敏感性和特异性分别为98.1%和100%、95.1%和98.6%、91.4%和94.9%、78.6%和97.9%。异烟肼、利福平、链霉素和乙胺丁醇的NRA与比例法的符合率分别为99.1%、97.3%、93.8%和95.6%。NRA是结核分枝杆菌对治疗结核病的两种最重要药物异烟肼和利福平进行药敏试验的一种简便、廉价和可靠的方法。NRA法药敏试验时间的缩短、灵敏度和特异性的提高对耐多药结核病的检测具有重要意义。关键词:药敏,耐多药结核病,NRA,比例法
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引用次数: 0
Health System Research: Development, Designs and Methods 卫生系统研究:发展、设计和方法
Q4 Medicine Pub Date : 2019-11-24 DOI: 10.37107/jhas.60
Tulsi Ram Bhandar
Health system research concerns with health system and its results provide the bases to managers, policy makers as well as community people to make evidence based decision. There are different interpretations of what a health system is. In narrow meaning, health system is considered the different levels of the health care services such as central level health care, state/ provincial level health care, regional/zonal/district level health care and local level health care. In broad aspect, health system covers different aspects of society such as socio-economic status, culture, religion, education, politics, public sector, private sectors which are the major determinants of social epidemiology. It is also a knowledge generation to improve how societies organise to achieve health goals and contributes to sound, socially relevant and ethically acceptable guidelines for more effective, efficient and sustainable health policies and systems. Key words: Health system research, development, designs, methods, healthcare research
卫生系统研究关注卫生系统及其结果,为管理者、政策制定者和社区民众做出循证决策提供了依据。对于什么是卫生系统有不同的解释。从狭义上讲,卫生系统被认为是不同层次的卫生保健服务,如中央一级卫生保健,国家/省级卫生保健,区域/区域/地区一级卫生保健和地方一级卫生保健。从广义上讲,卫生系统涵盖社会的不同方面,如社会经济地位、文化、宗教、教育、政治、公共部门、私营部门,这些都是社会流行病学的主要决定因素。它也是一种知识的产生,可以改进社会如何组织起来实现卫生目标,并有助于制定健全的、与社会相关的和道德上可接受的准则,以促进更有效、高效和可持续的卫生政策和系统。关键词:卫生系统研究、开发、设计、方法、卫生保健研究
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引用次数: 2
Impact of Socio-Cultural Factors on Antenatal Practices 社会文化因素对产前实践的影响
Q4 Medicine Pub Date : 2019-11-24 DOI: 10.37107/jhas.48
Madhu Ghimire, G. Gautam, M. Ghimire, Tara Sharma, B. Sharma
The provision for special care during pregnancy through public health services is considered by World Health organization as a part of reproductive health right of women which, however, is not achieved till date due to socio-cultural factors resulting increased maternal morbidity and mortality. The objective of this study is to observe impact of socio-cultural factors on antenatal practices. The study was conducted in three Village Development Committees (Dakhaquady, Khaira, and Belbash) of Pyuthan district. During the period Cross-sectional study was followed. Two hundred forty six respondents were selected through simple random procedure. Interview schedules were used to collect information from respondents. Data were tabulated in Microsoft Excel spreadsheet and analyzed using SPSS 11.5. The mean age of first pregnancy among respondents was 18.75 years. Forty-two percent of them were illiterate and 76.90 percent respondents were involved in agricultural field work. Early marriage and pregnancy systems were most common in study population. Socio-cultural factors were strongly associated with ANC practices. Key words: ANC practices, early marriage, early pregnancy, Pyuthan, socio-cultural factors, traditional beliefs.
世界卫生组织认为,通过公共卫生服务在怀孕期间提供特别护理是妇女生殖健康权的一部分,但由于社会文化因素导致孕产妇发病率和死亡率上升,这一权利迄今尚未实现。本研究的目的是观察社会文化因素对产前实践的影响。这项研究是在皮丹县的三个村发展委员会(Dakhaquady、Khaira和Belbash)进行的。在此期间,我们进行了横断面研究。通过简单的随机程序选出246名受访者。访谈时间表用于收集受访者的信息。数据以Microsoft Excel电子表格制表,并使用SPSS 11.5进行分析。受访者首次怀孕的平均年龄为18.75岁。其中42%的人不识字,76.90%的人从事农业田间劳动。早婚和早孕制度在研究人群中最为常见。社会文化因素与非国大的做法密切相关。关键词:非国大习俗,早婚,早孕,皮丹,社会文化因素,传统信仰
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引用次数: 0
Medication Adherence and Blood Pressure Control among Hypertensive Patients of Pokhara Valley 博卡拉山谷地区高血压患者的药物依从性与血压控制
Q4 Medicine Pub Date : 2019-11-24 DOI: 10.37107/jhas.59
Gulam Muhammad Khan, Raj Kumar Thapa, A. Khakurel, G. Shrestha, N. Katila, S. Bhurtel
Hypertension is a chronic medical condition which remains inadequately managed everywhere. Medication adherence (MA) is a critical parameter for achieving strict blood pressure (BP) control in patients undergoing antihypertensive therapy (AHT). Good MA is also a key factor determining the success of preventive measures for cardiovascular (CV) risk reduction. So, the objective of this research was to study the medication adherence and blood pressure control among hypertensive patients of Pokhara Valley. A randomized community-based prospective study was conducted in different places of Pokhara. In the study, a structured questionnaire i.e. Brief medication questionnaire (BMQ) with a high sensitivity and specificity was used. Out of 79 respondents enrolled, most of the respondents (n=51, 64.6%) were shown to be non-adherent and only 28 (35.4%) were adherent to the prescribed AHT. The study showed that among adherers, 75% were undergoing monotherapy and 64.3% (n=18) had their BP under control. The comparison between BP control and the different occupation showed that the highest percentage of BP control was achieved in housewives. Most of the respondents were non-adherent to the prescribed AHT which requires special attention of the healthcare providers. Key words: Hypertension, medication adherence, brief medication questionnaire, anti-hypertensive therapy
高血压是一种慢性疾病,在世界各地仍未得到充分管理。药物依从性(MA)是接受降压治疗(AHT)患者实现严格控制血压(BP)的关键参数。良好的MA也是决定心血管(CV)风险降低预防措施成功与否的关键因素。因此,本研究的目的是研究博卡拉山谷高血压患者的药物依从性和血压控制。在博卡拉不同地区进行了一项随机社区前瞻性研究。本研究采用敏感性和特异性较高的结构化问卷——BMQ (Brief medication questionnaire)。在登记的79名应答者中,大多数应答者(n= 51,64.6%)显示为不坚持,只有28人(35.4%)坚持规定的AHT。研究显示,75%的依从者正在接受单药治疗,64.3% (n=18)的血压得到控制。不同职业的血压控制率比较表明,家庭主妇的血压控制率最高。大多数应答者不遵守规定的辅助治疗,这需要卫生保健提供者的特别注意。关键词:高血压,药物依从性,简短用药问卷,降压治疗
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引用次数: 9
Survey on Medicinal Plants used for Anti-diabetic Activity in Kaski District, Nepal 尼泊尔卡斯基地区抗糖尿病药用植物调查
Q4 Medicine Pub Date : 2019-11-21 DOI: 10.37107/JHAS.16
P. Shrestha, Nirmala Jamarkattel-Pandit
Nepal is a Himalayan country with disproportionately rich cultural and ethnic diversity, recorded of over 700 species of medicinal plants. Most of the people in rural area rely on herbal medicines to treat diabetes mellitus since they have limited resources and do not have access to modern treatment. The main aim of the study was to search the medicinal plants used for diabetes mellitus for the development of evidence based medicine. The study was conducted in five different places of Kaski District, Nepal with two hundred numbers of respondents. It was found that majority of them had good knowledge on traditional use of the plants for diabetes and had been using 52 plant species of 35 families in which Asparagus racemosus, Momordica charantia, Berberis aristata, Azadiracta indica, Holorhena pubences, Eugenia jambolana, Aegle marmelous and Gymnema sylvestre are the most widely used plants for antidiabetic purposes. Key words: Diabetes Mellitus, Medicinal Plants, Kaski District, Survey, Respondents
尼泊尔是一个喜玛拉雅山上的国家,拥有丰富的文化和种族多样性,有记录的药用植物超过700种。农村地区由于资源有限,无法获得现代化的治疗手段,大多数人依靠草药治疗糖尿病。本研究的主要目的是寻找治疗糖尿病的药用植物,以开发循证医学。这项研究是在尼泊尔卡斯基区的五个不同地方进行的,有200名受访者。调查结果显示,大多数调查对象对糖尿病植物的传统用途有一定的了解,并已使用了35科52种植物,其中以总状芦笋、苦瓜、小檗、印楝、短毛莲、金针藤、金针藤等为应用最广泛的植物。关键词:糖尿病;药用植物;卡斯基区
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引用次数: 2
Nutritional Status and its Associated Factors among Adolescents 青少年营养状况及其相关因素
Q4 Medicine Pub Date : 2019-11-21 DOI: 10.37107/jhas.18
A. Puri, Chiranjivi Adhikari
Maintaining energy balance among adolescents is of paramount challenge. Many adolescent boys and girls in developing countries enter either with negative or positive energy balances and both results into diseases and ill health. The study aimed to find the nutritional status and its associated factors in school going adolescents of Lekhnath Municipality of Kaski district of Nepal. Cross-sectional analytical study was conducted in public secondary schools of Lekhnath Municipality among 356 adolescents through cluster random sampling. OMRON body fat analyzer, bathroom scale, stadiometer were used for body fat percentage and BMI calculation. Pretested self-administrable questionnaire was used to assess nutritional factors of adolescents. Frequency tabulation, chi square test, binomial logistic regression and correlation were done for statistical analyses. Prevalence of underweight, overweight, stunting and thinness were found to be 50.6 percent, 11 percent, 2.5 percent and 16.9 percent respectively. According to body fat percentage- 49.4 percent, 32.2 percent and 18.4 percent were lean, normal and obese. Seventy percent of adolescents performed high physical activity followed by 31.7 and 14 percent moderate to low physical activity. Only 3.1 percent of adolescents consumed recommended daily intake of fruits and vegetables. Age, gender, religion, ethnicity, family type and availability of kitchen garden were associated with body mass index. Early adolescent, male, Hindu, students from nuclear families were found more underweight than their counterparts. Underweight is more prevalent than overweight among adolescents. Health promoting programs including kitchen garden and fruits and vegetables intake are recommended. Keywords: Nutrition, adolescent, factor, overweight, underweight, developing, physical
维持青少年之间的能量平衡是最重要的挑战。发展中国家的许多青少年男女进入社会时要么是负能量平衡,要么是正能量平衡,两者都会导致疾病和不健康。本研究旨在了解尼泊尔卡斯基区列克纳特市学龄青少年的营养状况及其相关因素。采用整群随机抽样的方法,在列克纳特市公立中学对356名青少年进行横断面分析研究。采用欧姆龙体脂仪、浴室称、体重计计算体脂率和BMI。采用预测自填问卷对青少年的营养因素进行评估。采用频率表、卡方检验、二项logistic回归及相关分析进行统计分析。体重不足、超重、发育迟缓和消瘦的患病率分别为50.6%、11%、2.5%和16.9%。从体脂率来看,49.4%、32.2%和18.4%分别为瘦、正常和肥胖。70%的青少年进行高强度的体育锻炼,其次是31.7%和14%的青少年进行中度到低强度的体育锻炼。只有3.1%的青少年食用了推荐的每日水果和蔬菜摄入量。年龄、性别、宗教、种族、家庭类型和是否有菜园与体重指数相关。青少年早期、男性、印度教徒和来自核心家庭的学生比同龄人体重更轻。在青少年中,体重不足比超重更为普遍。健康促进计划包括厨房菜园和水果蔬菜的摄入。关键词:营养,青少年,因素,超重,体重不足,发育,身体
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引用次数: 0
Diurnal Variation of Serum 25 (OH) D3 , Calcium and Phosphorus in Type 2 Diabetes Mellitus 2型糖尿病患者血清25 (OH) D3、钙、磷的日变化
Q4 Medicine Pub Date : 2019-11-21 DOI: 10.37107/jhas.4
R. Shakya, B. Neupane, D. P. Bhandari
Diurnal variation, an outgrowth of chronobiology, is the inferential statistical mapping of structures in variables; in and around us, consisting of rhythms chaos and trends. Type-2 diabetes mellitus is a metabolic disorder with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action or both. The diurnal time structure of serum 25(OH) D3 and calcium may relate to the prevention and chronotherapeutic efficacy and management of type 2 diabetes mellitus. To our knowledge, the diurnal variation of serum 25(OH) D3 has not yet been reported in type 2 diabetic patients. The present study was planned to evaluate the diurnal variation of serum 25(OH) D3 , calcium and phosphorus levels in type 2 diabetic patients. Ten clinically healthy volunteers and ten diagnosed patients of type 2 diabetes mellitus of similar age groups were synchronized for one month with diurnal activity from about 06:00 to about 22:00 and nocturnal rest. All subjects took their meals three times daily without any change in their usual fluid intake. Blood sample were collected into plain and sterile vials under quality control procedures from each participant at every 6 hour. Serum 25(OH) D3 , serum Ca++, serum PO4 , FBS and PPBS levels were estimated. A marked diurnal variation in serum 25(OH) D3 was recorded in healthy subjects (P=0.030). Similarly, a circadian rhythm of borderline statistical significance was also recorded for vitamin D in diabetic patients (P=0.083) and in healthy participants for serum calcium (P=0.070), phosphorus (P=0.102), and the calcium-phosphorus ratio (P=0.091) by the Two way ANOVA analysis. In addition, the amplitude and acrophase differed from healthy participants in diabetic patients for studied variables with a change of MESOR for calcium-phosphorus ratio. Mapping the broader time structure of different physiological variables investigated herein may be helpful in understanding the treatment and prevention of diabetic mellitus. Key words: Serum 25(OH) D3 , Calcium, Phosphorous, Type 2 Diabetes mellitus
日变化是时间生物学的产物,是变量结构的推断统计映射;在我们内心和周围,由节奏、混乱和趋势组成。2型糖尿病是由于胰岛素分泌、胰岛素作用或两者都有缺陷而引起的碳水化合物、脂肪和蛋白质代谢紊乱的代谢性疾病。血清25(OH) D3和钙的昼夜时间结构可能与2型糖尿病的预防、时间治疗效果和管理有关。据我们所知,2型糖尿病患者血清25(OH) D3的日变化尚未见报道。本研究旨在评估2型糖尿病患者血清25(OH) D3、钙和磷水平的日变化。10名临床健康志愿者和10名年龄相近的2型糖尿病确诊患者同步进行为期1个月的活动,时间为06:00 ~ 22:00左右,夜间休息。所有的研究对象每天吃三顿饭,他们通常的液体摄入量没有任何变化。根据质量控制程序,每6小时采集一次参与者的血液样本,装入普通和无菌的小瓶中。测定血清25(OH) D3、Ca++、PO4、FBS和PPBS水平。健康受试者血清25(OH) D3的日变化显著(P=0.030)。同样,通过双向方差分析,糖尿病患者的维生素D昼夜节律(P=0.083)和健康参与者的血清钙(P=0.070)、磷(P=0.102)和钙磷比(P=0.091)也记录了具有临界统计学意义的昼夜节律。此外,在MESOR钙磷比变化的研究变量中,糖尿病患者的振幅和端相与健康受试者不同。绘制本文所研究的不同生理变量的更广泛的时间结构可能有助于了解糖尿病的治疗和预防。关键词:血清25(OH) D3,钙,磷,2型糖尿病
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引用次数: 0
Educational Satisfaction of Public Health Undergraduates: A cross sectional study in Nepal 尼泊尔公共卫生本科生教育满意度横断面研究
Q4 Medicine Pub Date : 2019-11-21 DOI: 10.37107/jhas.33
S. Subedi, Hari Prasad Kaphle, R. Neupane, R. Rajbhandari
Educational satisfaction determines the quality of education system which interns affects the performance and achievement of students during study and finally future professional competencies. The objectives of this study were to identify the perceived status of satisfaction on educational conditions and teaching-learning environment and associated factors of it among the public health undergraduates. This was the descriptive cross sectional study carried out among 384 undergraduate students of public health studying in final year/semester of three universities of Nepal using self administered questionnaire. The result of the study showed that satisfactions on various components are below than average. Among the satisfied components, the major were in case of duration of course (46.1%), teaching skills of part time teacher (39.1%), curriculum and overall course of study (36.2%), research/dissertation guidance (34.6%) and teaching skills of full time teacher (33.6%). The satisfaction level was least in case of result timing/procedure (14.1%), tuition fee (23.7%), examination procedure (26.3%) and evaluation system (27.1%). Ethnicity, educational background, course duration, types of teacher, availability of books in library and availability of research papers/journals were found associated with educational satisfaction. The study concluded low educational satisfaction among public health under graduate students. It is recommended to take immediate action for improvements of different components of education system like curriculum structure & contains, teaching methodology, teaching learning materials, admission and examination procedure. Key words: Public health; Education; undergraduate course; satisfaction; Nepal
教育满意度决定了教育体系的质量,实习生在学习期间的表现和成就,最终影响到未来的职业能力。摘要本研究旨在了解公共卫生专业本科生对教育条件和教与学环境满意度的感知状况及其相关因素。这是一项描述性横断面研究,采用自填问卷对尼泊尔三所大学384名公共卫生专业本科生进行调查。研究结果显示,各方面的满意度都低于平均水平。在专业满意的组成部分中,课程时长(46.1%)、兼职教师的教学技能(39.1%)、课程与整体课程(36.2%)、研究/论文指导(34.6%)和专职教师的教学技能(33.6%)是专业满意的组成部分。满意度最低的是成绩时间/程序(14.1%)、学费(23.7%)、考试程序(26.3%)和评价制度(27.1%)。种族、教育背景、课程持续时间、教师类型、图书馆书籍的可得性和研究论文/期刊的可得性被发现与教育满意度有关。研究发现,公共卫生本科学生的教育满意度较低。建议立即采取行动改善教育系统的不同组成部分,如课程结构和内容、教学方法、教学材料、入学和考试程序。关键词:公共卫生;教育;本科;满意度;尼泊尔
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引用次数: 0
Knowledge and Practice on Reproductive Heath Rights among Married Women in Nepal 尼泊尔已婚妇女生殖健康权利的知识和实践
Q4 Medicine Pub Date : 2019-11-21 DOI: 10.37107/jhas.36
Rajesh Kumar Yadav, Nand Ram Gahatraj, Dipendra Kumar Yadav, S. Marahatta
Reproductive health rights ensure that people are able to have satisfying and safe sex life and that they have the capacity to reproduce with freedom to decide, when and how often to do so. This study aimed to assess the knowledge and practices on reproductive health rights among madhesi married women. A descriptive cross-sectional study was conducted among 384 madhesi married women of reproductive age in Sarlahi district conducted from July 2014 to December 2014. Data were entry in Epidata software and analyzed by SPSS 20 version. Of the total 384 respondents most of them (39.3%) were of age group 25-35 years from different ethnic groups. Most were simple illiterate (65.6%) and housewife (60.9%) very few of respondents were knowledge about reproductive health rights (37%) and not statistical significant relationship with level of knowledge and level of practice (χ 1 2 =2.036, p=0.154). Level of knowledge was statistically significant relationship with educational level (χ 4 2 = 43.983 p<0.001). Use of FP services have statistically significant relationship with Age group (p<0.001). The level of knowledge and level of practice on RHRs is still very low in Madhesi women. Keywords: Reproductive health rights; Madhesi women; practice
生殖健康权确保人们能够拥有满意和安全的性生活,并确保他们有能力自由决定生育的时间和频率。本研究旨在评估马德西族已婚妇女关于生殖健康权利的知识和做法。对2014年7月至2014年12月在Sarlahi区384名madhesi已婚育龄妇女进行描述性横断面研究。数据用Epidata软件录入,用SPSS 20进行分析。在384名受访者中,大多数(39.3%)为25-35岁年龄段,来自不同民族。调查对象中以单纯文盲(65.6%)和家庭主妇(60.9%)居多,对生殖健康权利有知识的比例极少(37%),与知识水平和实践水平无统计学意义(χ 2 =2.036, p=0.154)。知识水平与受教育程度有统计学意义(χ 2 = 43.983 p<0.001)。计划生育服务的使用与年龄组有统计学意义(p<0.001)。马德西族妇女对生殖健康风险的知识水平和实践水平仍然很低。关键词:生殖健康权;曾女人;实践
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引用次数: 3
Extended Spectrem Beta Lactamases among Multi Drug Resistant Gram Negetive Bacilli Causing Urinry Tract Infection 尿路感染多重耐药革兰氏阴性杆菌中β -内酰胺酶的广谱分析
Q4 Medicine Pub Date : 2019-11-21 DOI: 10.37107/jhas.30
G. Bhattarai, D. Shrestha, B. Tiwari
Extended Spectrum Beta Lactamases (ESBL), the main cause of resistance to broad spectrum β-lactams, among uropathogenic bacteria have increased over time raising a global concern in the therapeutic management of infections caused by these organisms. The study was carried out in Janamaitri Hospital, Kathmandu between December 2012 to May 2013 with an objective to determine the status of ESBL producing Gram negative bacilli isolated from the urine sample, collected from patients suspected of urinary tract infection. Gram negative bacilli isolated were tested for the presence of ESBL by combined disk and antibiotic susceptibility by Kirby Bauer disc diffusion method following Clinical and Laboratory Standard Institute guidelines. Among the total 1105 mid-stream urine samples, 256 Gram negative bacilli were isolated. By screening test using third generation cephalosporins, 156 isolates were screened as ESBL producers and 91 isolates were positive for ESBL test by combined disk method. Among the 91 (35.55%) ESBL producers, 70 (39.32%) Escherichia coli, 16 (44.44%) Klebsiella pneumoniae, and 5 (33.33%) Pseudomonas aeruginosa were found to be ESBL producers. Majority of ESBL producer showed resistance to ampicillin, co-trimoxazole, norfloxacin followed by ofloxacin. imipenem, amikacin and nitrofurantoin seemed to be the agent of choice for urinary tract infections when ESBL producers are susceptible to it. ESBL production found in these Gram negative bacilli with resultant microbial resistance to available cephalosporins and other agents may pose difficulties with the choice of therapeutic options for the treatment of severe infections. Keywords: UTI, Extended Spectrum Beta Lactamases, Gram negative bacilli
随着时间的推移,尿路病原菌中广谱β-内酰胺耐药的主要原因——广谱β-内酰胺酶(ESBL)的数量增加,引起了全球对这些微生物引起的感染治疗管理的关注。该研究于2012年12月至2013年5月在加德满都Janamaitri医院进行,目的是确定从疑似尿路感染患者收集的尿液样本中分离出的产生革兰氏阴性杆菌的ESBL状态。根据临床和实验室标准协会的指南,采用Kirby Bauer圆盘扩散法联合检测分离的革兰氏阴性杆菌是否存在ESBL,并对抗生素进行敏感性检测。在1105份中游尿液样本中,检出革兰氏阴性杆菌256株。经第三代头孢菌素筛选,156株菌株为ESBL产生菌,91株菌株联合圆盘法ESBL检测阳性。91株ESBL产生菌中,大肠杆菌70株(39.32%)、肺炎克雷伯菌16株(44.44%)、铜绿假单胞菌5株(33.33%)为ESBL产生菌。大多数ESBL生产者对氨苄西林、复方新诺明、诺氟沙星耐药,其次是氧氟沙星。亚胺培南、阿米卡星和呋喃妥因似乎是易受ESBL生产者感染的尿路感染的首选药物。在这些革兰氏阴性杆菌中发现ESBL产生,并导致微生物对现有头孢菌素和其他药物产生耐药性,这可能给选择治疗严重感染的治疗方案带来困难。关键词:UTI,广谱β -内酰胺酶,革兰氏阴性杆菌
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