Pub Date : 2019-06-01DOI: 10.4167/JBV.2019.49.2.53
Eun Seon Chung, So Yeon Kim, K. Ko
We investigated the effect of toxin-antitoxin (TA) systems in bla CTX-M-15 -bearing plasmids of Klebsiella pneumoniae on persister formation. The persister formation rate was notably high in transconjugants in plasmids bearing TA system than the transconjugants in plasmids bearing no TA systems. Activation of relA and spoT expression was higher in transconjugants with plasmids bearing TA systems. Thus, TA systems in plasmids may contribute to the maintenance of bla CTX-M-15 -bearing plasmids and host survival via persister formation. Plasmids were obtained from four CTX-M-15-producing K. pneumoniae ST11 clinical isolates that were collected from patients in Hong Kong, Malaysia, India, and Thailand (HK02-026, M16-13, IN03-01, and TH02-34, respectively). Their whole plasmid sequences were determined previously (GenBank accession numbers, KY751926, KY751925, KY499796, and KY499797) (14). While TA genes, such as pemIK, mok-hok, and vagCD, were identified on the bla CTX-M-15 -bearing plasmids of HK02-026 and M16-13 (TA+), the bla CTX-M-15 -bearing plasmids of IN03-01 and TH02-34 had no TA genes (TA – ). Then, recovered plasmids were transferred to E. coli J53 cells, as described previously (6). In vitro antimicrobial susceptibility testing was performed by the broth microdilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines (15), and susceptibility was defined according to CLSI breakpoints. E. coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were used as control strains.
{"title":"Association Between Toxin-antitoxin Systems on Plasmids and Persister Formation in CTX-15-producing Klebsiella pneumoniae ST11 Isolates","authors":"Eun Seon Chung, So Yeon Kim, K. Ko","doi":"10.4167/JBV.2019.49.2.53","DOIUrl":"https://doi.org/10.4167/JBV.2019.49.2.53","url":null,"abstract":"We investigated the effect of toxin-antitoxin (TA) systems in bla CTX-M-15 -bearing plasmids of Klebsiella pneumoniae on persister formation. The persister formation rate was notably high in transconjugants in plasmids bearing TA system than the transconjugants in plasmids bearing no TA systems. Activation of relA and spoT expression was higher in transconjugants with plasmids bearing TA systems. Thus, TA systems in plasmids may contribute to the maintenance of bla CTX-M-15 -bearing plasmids and host survival via persister formation. Plasmids were obtained from four CTX-M-15-producing K. pneumoniae ST11 clinical isolates that were collected from patients in Hong Kong, Malaysia, India, and Thailand (HK02-026, M16-13, IN03-01, and TH02-34, respectively). Their whole plasmid sequences were determined previously (GenBank accession numbers, KY751926, KY751925, KY499796, and KY499797) (14). While TA genes, such as pemIK, mok-hok, and vagCD, were identified on the bla CTX-M-15 -bearing plasmids of HK02-026 and M16-13 (TA+), the bla CTX-M-15 -bearing plasmids of IN03-01 and TH02-34 had no TA genes (TA – ). Then, recovered plasmids were transferred to E. coli J53 cells, as described previously (6). In vitro antimicrobial susceptibility testing was performed by the broth microdilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines (15), and susceptibility was defined according to CLSI breakpoints. E. coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were used as control strains.","PeriodicalId":39739,"journal":{"name":"Journal of Bacteriology and Virology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4167/JBV.2019.49.2.53","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45125719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.4167/jbv.2019.49.2.45
H. Lee, Jin-Won Song
the order Bunyavirales, previously family Bunyaviridae, was by new order of families including Hantaviridae family, now the on of Hantaviridae is now a family of the order Bunyavirales, and the prototype virus species is Hantaan orthohantavirus. The family Hantaviridae is divided into four subfamilies including Mammantavirinae, Repantavirinae, Actantavirinae and Agantavirinae. The subfamily Mammantavirinae is divided into four genera including Orthohantavirus, Loanvirus, Mobatvirus and Thottimvirus. The four Hantavirus species have been found in Korea including three Orthohantaviruses (Hantaan orthohantavirus, Seoul orthohantavirus and Jeju orthohantavirus) and one Thottimvirus (Imjin thottimvirus).
{"title":"Our Hantaan Virus Became a New Family, Hantaviridae in the Classification of Order Bunyavirales. It will Remain as a History of Virology","authors":"H. Lee, Jin-Won Song","doi":"10.4167/jbv.2019.49.2.45","DOIUrl":"https://doi.org/10.4167/jbv.2019.49.2.45","url":null,"abstract":"the order Bunyavirales, previously family Bunyaviridae, was by new order of families including Hantaviridae family, now the on of Hantaviridae is now a family of the order Bunyavirales, and the prototype virus species is Hantaan orthohantavirus. The family Hantaviridae is divided into four subfamilies including Mammantavirinae, Repantavirinae, Actantavirinae and Agantavirinae. The subfamily Mammantavirinae is divided into four genera including Orthohantavirus, Loanvirus, Mobatvirus and Thottimvirus. The four Hantavirus species have been found in Korea including three Orthohantaviruses (Hantaan orthohantavirus, Seoul orthohantavirus and Jeju orthohantavirus) and one Thottimvirus (Imjin thottimvirus).","PeriodicalId":39739,"journal":{"name":"Journal of Bacteriology and Virology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4167/jbv.2019.49.2.45","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43038785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.4167/jbv.2019.49.2.59
Sang-Hun Park, Young-Hee Jin, Mi-Jin Ahn, Sunghee Han, H. Kim, Jin Seok Kim, Joo-Hyun Park, Chae-Kyu Hong, Sook Park, Ah-Ryung Oh, Jibho Lee, Il-Young Kim, Yong-Seoung Shin
Legionella species are abundant in the built environment and are increasingly recognized as a cause of Legionnaires ’ disease (LD). As the number of cases of Legionnaires ’ disease acquired by local communities in the Seoul metropolitan area in Korea has been increased, there was concern that changes in environmental factors could affect disease outbreaks. We described the association between climatic variables and occurrence of legionellosis in Korea and Legionella detection rate in Seoul area. A total of 418 cases of legionellosis were reported between 2014 and 2017. There was a seasonal peak in summer. LD continuously occurred from early spring to winter every year and rapidly increased in summer. In the regression analysis, the primary variables of interest- PM2.5 ( g/m 3 ), NO 2 (ppb), and a number of the date of issue O 3 warning were not significant except for average temperature (R 2 =0.8075). The Legionella detection rate in Seoul, Korea showed a trend similar to precipitation (P=0.708, ANOVA). A relatively high proportion of Legionella detection rate was shown, especially cooling tower (17.7%) and public bath (19.3%). This finding is in line with current understanding of the ecological profile of this pathogen and supports the assertion that legionellosis occurs through contamination of water
{"title":"Epidemiology ofLegionellaand Climatic Variables in Seoul, Korea","authors":"Sang-Hun Park, Young-Hee Jin, Mi-Jin Ahn, Sunghee Han, H. Kim, Jin Seok Kim, Joo-Hyun Park, Chae-Kyu Hong, Sook Park, Ah-Ryung Oh, Jibho Lee, Il-Young Kim, Yong-Seoung Shin","doi":"10.4167/jbv.2019.49.2.59","DOIUrl":"https://doi.org/10.4167/jbv.2019.49.2.59","url":null,"abstract":"Legionella species are abundant in the built environment and are increasingly recognized as a cause of Legionnaires ’ disease (LD). As the number of cases of Legionnaires ’ disease acquired by local communities in the Seoul metropolitan area in Korea has been increased, there was concern that changes in environmental factors could affect disease outbreaks. We described the association between climatic variables and occurrence of legionellosis in Korea and Legionella detection rate in Seoul area. A total of 418 cases of legionellosis were reported between 2014 and 2017. There was a seasonal peak in summer. LD continuously occurred from early spring to winter every year and rapidly increased in summer. In the regression analysis, the primary variables of interest- PM2.5 ( g/m 3 ), NO 2 (ppb), and a number of the date of issue O 3 warning were not significant except for average temperature (R 2 =0.8075). The Legionella detection rate in Seoul, Korea showed a trend similar to precipitation (P=0.708, ANOVA). A relatively high proportion of Legionella detection rate was shown, especially cooling tower (17.7%) and public bath (19.3%). This finding is in line with current understanding of the ecological profile of this pathogen and supports the assertion that legionellosis occurs through contamination of water","PeriodicalId":39739,"journal":{"name":"Journal of Bacteriology and Virology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4167/jbv.2019.49.2.59","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43027986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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