Blood routine examination is an inexpensive, routinely performed clinical project, which includes many blood parameters. Hepatitis B virus (HBV) infection may have significant effects on blood parameters in decompensated patients with hepatitis B cirrhosis. However, details were not quite clear yet. A total of 207 HBV‐related decompensated cirrhosis (HBV‐DeCi) patients were included in this retrospective analysis. The included patients were subdivided into three groups: 44 patients with HBsAg, HBeAg, and HbcAb positive were set as Group I; 122 patients with HBsAg, HBeAb, and HbcAb positive were set as Group II; 41 patients with HBsAb, HBeAb, and HbcAb positive were set as Group III. Blood parameters, including platelet (PLT) count, mean platelet volume (MPV), lymphocyte count, leukocyte count and MPV/lymphocyte ratio (MPVLR), lymphocyte/ leukocyte ratio (LLR), platelet/leukocyte ratio (PLR) were analyzed respectively. The correlation between mentioned parameters and the HBV infection were characterized respectively. The values of PLT, MPVLR, and PLR in Group I and II were lower than that in Group III. Group II has a much higher LLR than that in Group I and III. HBV does have significantly effect on blood parameters in HBV‐DeCi patients. Effective treatment must be applied to achieve cirrhosis recompensation promptly.
{"title":"Platelets, mean platelet volume, lymphocytes, leukocytes, and ratios of them altered in patients with hepatitis B virus‐related decompensated cirrhosis","authors":"Changzhi Xu, Yanhua Yi, Zhizhi Xie, Donglin Zhu, Dannier Abuduwaili, Zhu Wang, Yun Xi","doi":"10.1002/prm2.12070","DOIUrl":"https://doi.org/10.1002/prm2.12070","url":null,"abstract":"Blood routine examination is an inexpensive, routinely performed clinical project, which includes many blood parameters. Hepatitis B virus (HBV) infection may have significant effects on blood parameters in decompensated patients with hepatitis B cirrhosis. However, details were not quite clear yet. A total of 207 HBV‐related decompensated cirrhosis (HBV‐DeCi) patients were included in this retrospective analysis. The included patients were subdivided into three groups: 44 patients with HBsAg, HBeAg, and HbcAb positive were set as Group I; 122 patients with HBsAg, HBeAb, and HbcAb positive were set as Group II; 41 patients with HBsAb, HBeAb, and HbcAb positive were set as Group III. Blood parameters, including platelet (PLT) count, mean platelet volume (MPV), lymphocyte count, leukocyte count and MPV/lymphocyte ratio (MPVLR), lymphocyte/ leukocyte ratio (LLR), platelet/leukocyte ratio (PLR) were analyzed respectively. The correlation between mentioned parameters and the HBV infection were characterized respectively. The values of PLT, MPVLR, and PLR in Group I and II were lower than that in Group III. Group II has a much higher LLR than that in Group I and III. HBV does have significantly effect on blood parameters in HBV‐DeCi patients. Effective treatment must be applied to achieve cirrhosis recompensation promptly.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"46 - 50"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46324275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dejun Wu, Zhou Yang, Leilei Sun, Yingjun Quan, Zhi-jun Min
Lymphovascular invasion (LVI) was considered to be important for metastasis of colorectal cancer (CRC). However, there was still no effective method to predict LVI before operation. Our research aimed to construct an artificial neural network (ANN) for the preoperative prediction of LVI. We obtained blood indexes and condition of LVI (confirmed by pathological examination) of 288 cases of CRC patients from a tertiary hospital in China. One hundred and eighty‐five CRC patients (training group) were randomly selected to establish neural network and logistic regression models. The remaining 103 cases of CRC patients received the test of ANN and logistic model (validation group). Receiver operating characteristics curve (ROC) and decision curve analysis (DCA) were performed to assess the accuracy of constructed model respectively. All procedures involving human participants were performed by the Shanghai Pudong Hospital ethical committee (2020 No. W2‐007) and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. All patients provided their written informed consent. In the training group, the area under the curve (AUC) of ANN was higher than that of the logistic model (0.832 vs 0.692). The ANN correctly predicted 92% cases of LVI, whereas the logistic model only predicted 56% cases. Similar results were also tested in the validation model. Our constructed ANN showed higher accuracy compared with the conventional linear model. The ANN‐based on blood indexes may provide value for preoperative prediction of LVI.
{"title":"Preoperative prediction of lymphovascular invasion of CRC by artificial neural network","authors":"Dejun Wu, Zhou Yang, Leilei Sun, Yingjun Quan, Zhi-jun Min","doi":"10.1002/prm2.12074","DOIUrl":"https://doi.org/10.1002/prm2.12074","url":null,"abstract":"Lymphovascular invasion (LVI) was considered to be important for metastasis of colorectal cancer (CRC). However, there was still no effective method to predict LVI before operation. Our research aimed to construct an artificial neural network (ANN) for the preoperative prediction of LVI. We obtained blood indexes and condition of LVI (confirmed by pathological examination) of 288 cases of CRC patients from a tertiary hospital in China. One hundred and eighty‐five CRC patients (training group) were randomly selected to establish neural network and logistic regression models. The remaining 103 cases of CRC patients received the test of ANN and logistic model (validation group). Receiver operating characteristics curve (ROC) and decision curve analysis (DCA) were performed to assess the accuracy of constructed model respectively. All procedures involving human participants were performed by the Shanghai Pudong Hospital ethical committee (2020 No. W2‐007) and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. All patients provided their written informed consent. In the training group, the area under the curve (AUC) of ANN was higher than that of the logistic model (0.832 vs 0.692). The ANN correctly predicted 92% cases of LVI, whereas the logistic model only predicted 56% cases. Similar results were also tested in the validation model. Our constructed ANN showed higher accuracy compared with the conventional linear model. The ANN‐based on blood indexes may provide value for preoperative prediction of LVI.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"62 - 68"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45689920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We established a Cetuximab‐resistant cell lines by high‐dose pulse method and searched for differentially expressed circular RNAs by RNA sequencing. Hundreds of circRNAs were altered between sensitive and resistant cells. Next, we chose six notably differential circRNAs and conducted quantitative real‐time PCR by specific primers. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that differentially expressed circular RNAs are enriched in some tumor‐related pathways, such as tumor transcription regulation, metabolism, PI3K‐Akt, mTOR, and other signaling pathways. Our results explored differentially expressed circular RNAs associated with Cetuximab to find new targets for Cetuximab resistance therapy.
{"title":"The analysis of the circRNAs in the progress of acquired resistance to Cetuximab","authors":"Liping Yin, Changwen Jing, Yesong Guo","doi":"10.1002/prm2.12066","DOIUrl":"https://doi.org/10.1002/prm2.12066","url":null,"abstract":"We established a Cetuximab‐resistant cell lines by high‐dose pulse method and searched for differentially expressed circular RNAs by RNA sequencing. Hundreds of circRNAs were altered between sensitive and resistant cells. Next, we chose six notably differential circRNAs and conducted quantitative real‐time PCR by specific primers. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that differentially expressed circular RNAs are enriched in some tumor‐related pathways, such as tumor transcription regulation, metabolism, PI3K‐Akt, mTOR, and other signaling pathways. Our results explored differentially expressed circular RNAs associated with Cetuximab to find new targets for Cetuximab resistance therapy.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"51 - 55"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48326581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of the study is to explore the effect of an ear health exercise on hearing loss of patients with nasopharyngeal carcinoma receiving radiotherapy. One hundred and seventeen patients diagnosed with nasopharyngeal carcinoma from June 2018 to January 2020 were selected as research subjects randomly divided into three groups. Thirty‐eight cases were in experimental group 1 and given an ear health exercise for 6 months, 38 cases were in experimental group 2 and given an ear health exercise for 12 months, and 41 cases in control group only received hearing health education. The hearing loss of three groups was compared before intervention and at 6 and 12 months after intervention. The value of the average audibility threshold of air conduction, the occurrence rate of abnormal acoustic immittance (ear), the rate of tympanic cavity effusion, and occur rate of secretory otitis media in two experimental groups were lower than control group among different time points. The above indicators of experimental group 2 were lower than those of group 1 (p < .05). The total effective rate of hearing improvement in experimental groups 1 and 2 was higher than that of control group after the intervention. Besides, the total effective rate in experimental group 2 was higher than that in group 1, and the difference was statistically significant (p < .05). There was no serious adverse reaction. An ear health exercise can reduce hearing loss of patients with nasopharyngeal carcinoma receiving radiotherapy. It can be used as daily rehabilitation measures to prevent or delay hearing loss for these patients.
{"title":"Effect of ear exercises on hearing loss in patients with nasopharyngeal carcinoma after radiotherapy","authors":"Juan Lu, Hui Zhang, D. Xu, Lingyun Shi, Jing Wen","doi":"10.1002/prm2.12073","DOIUrl":"https://doi.org/10.1002/prm2.12073","url":null,"abstract":"The objective of the study is to explore the effect of an ear health exercise on hearing loss of patients with nasopharyngeal carcinoma receiving radiotherapy. One hundred and seventeen patients diagnosed with nasopharyngeal carcinoma from June 2018 to January 2020 were selected as research subjects randomly divided into three groups. Thirty‐eight cases were in experimental group 1 and given an ear health exercise for 6 months, 38 cases were in experimental group 2 and given an ear health exercise for 12 months, and 41 cases in control group only received hearing health education. The hearing loss of three groups was compared before intervention and at 6 and 12 months after intervention. The value of the average audibility threshold of air conduction, the occurrence rate of abnormal acoustic immittance (ear), the rate of tympanic cavity effusion, and occur rate of secretory otitis media in two experimental groups were lower than control group among different time points. The above indicators of experimental group 2 were lower than those of group 1 (p < .05). The total effective rate of hearing improvement in experimental groups 1 and 2 was higher than that of control group after the intervention. Besides, the total effective rate in experimental group 2 was higher than that in group 1, and the difference was statistically significant (p < .05). There was no serious adverse reaction. An ear health exercise can reduce hearing loss of patients with nasopharyngeal carcinoma receiving radiotherapy. It can be used as daily rehabilitation measures to prevent or delay hearing loss for these patients.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"87 - 94"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45907502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xingyu Jia, Wenchao Chen, Wei Chen, Yuxiang Liao, Jing Zhou, L. Yuan, Huiran Lin, Jun Bian
Gliomas, the most common type of primary intracranial tumor, arise from the brain's glial cells and constitute 30% of all brain and central nervous system tumors. MiR‐34b/c plays an important role in colorectal cancer and other cancers, but its role in glioma is unknown. In this study, we attempted to assess the effect of rs4938723 T > C in miR‐34b/c on the susceptibility of glioma Chinese children. Odds ratios and 95% confidence intervals (CIs) were calculated to evaluate the association between the selected polymorphism and glioma susceptibility. Our results showed that there is no significant association between this polymorphism and the risk of glioma in Chinese children. However, stratified analysis showed that the rs4938723 TC/CC genotype significantly reduced the risk of glioma in participants under 60 months of age (adjusted OR = 0.56, 95% CI = 0.35–0.88, p = .013). Overall, our study indicates that miR‐34b/c rs4938723 T > C polymorphism may have a weak influence on glioma susceptibility. Nevertheless, these findings need to be validated in well‐designed studies with larger sample sizes and different populations.
胶质瘤是最常见的原发性颅内肿瘤，起源于大脑的胶质细胞，占所有脑和中枢神经系统肿瘤的30%。MiR - 34b/c在结直肠癌和其他癌症中发挥重要作用，但在胶质瘤中的作用尚不清楚。在这项研究中，我们试图评估miR - 34b/ C中rs4938723 T > C对中国儿童胶质瘤易感性的影响。计算比值比和95%置信区间(ci)来评估所选多态性与胶质瘤易感性之间的关联。我们的研究结果显示，这种多态性与中国儿童患胶质瘤的风险之间没有显著的关联。然而，分层分析显示，rs4938723 TC/CC基因型显著降低了60月龄以下参与者发生胶质瘤的风险(调整后OR = 0.56, 95% CI = 0.35-0.88, p = 0.013)。总之，我们的研究表明miR - 34b/c rs4938723 T > c多态性可能对胶质瘤易感性有微弱的影响。然而，这些发现需要在设计良好的研究中进行验证，这些研究需要更大的样本量和不同的人群。
{"title":"Effect of miR‐34b/c rs4938723 T > C on pediatric glioma susceptibility","authors":"Xingyu Jia, Wenchao Chen, Wei Chen, Yuxiang Liao, Jing Zhou, L. Yuan, Huiran Lin, Jun Bian","doi":"10.1002/prm2.12067","DOIUrl":"https://doi.org/10.1002/prm2.12067","url":null,"abstract":"Gliomas, the most common type of primary intracranial tumor, arise from the brain's glial cells and constitute 30% of all brain and central nervous system tumors. MiR‐34b/c plays an important role in colorectal cancer and other cancers, but its role in glioma is unknown. In this study, we attempted to assess the effect of rs4938723 T > C in miR‐34b/c on the susceptibility of glioma Chinese children. Odds ratios and 95% confidence intervals (CIs) were calculated to evaluate the association between the selected polymorphism and glioma susceptibility. Our results showed that there is no significant association between this polymorphism and the risk of glioma in Chinese children. However, stratified analysis showed that the rs4938723 TC/CC genotype significantly reduced the risk of glioma in participants under 60 months of age (adjusted OR = 0.56, 95% CI = 0.35–0.88, p = .013). Overall, our study indicates that miR‐34b/c rs4938723 T > C polymorphism may have a weak influence on glioma susceptibility. Nevertheless, these findings need to be validated in well‐designed studies with larger sample sizes and different populations.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"82 - 86"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42050862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inflammation of the thyroid gland is referred to as thyroiditis and is associated with inflammation. Recent studies found significant association between thyroiditis and novel inflammatory and metabolic markers, as well as C‐reactive protein (CRP). CRP/lymphocyte count ratio (CLR) is a novel inflammatory marker that associated with various conditions and has not been studied in thyroiditis, yet. We aimed to investigate CRP to lymphocyte count ratio in patients with thyroiditis and to compare to those in healthy subjects. Patients with thyroiditis that presented to internal medicine outpatient clinics of our institution between January 2019 and August 2021 were enrolled to the retrospective study. Healthy volunteers were enrolled as control group. CLR of the thyroiditis and control groups were compared. Median CLR of the thyroiditis and control groups were 3.14 (0.14%–38)% and 0.4 (0.03–8.86)%, respectively (p < .001). The sensitivity and specificity of CLR > 0.43% in detecting thyroiditis were 92% and 58%, respectively (AUC: 0.88, p < .001, 95% CI: 0.85–0.92).CLR was significantly and positively correlated with free T4 (FT4) (r = .18, p < .001) and inversely correlated with thyroid stimulating hormone (TSH) (r = −.52, p = .003) levels. In conclusion, we suggest that high CLR levels may yield additional diagnostic value in patients with thyroiditis.
{"title":"C‐reactive protein to LymphocytE count ratio could be a reliable mArkeR of thyroiditis; the CLEAR‐T study","authors":"M. Demirkol, G. Aktas","doi":"10.1002/prm2.12065","DOIUrl":"https://doi.org/10.1002/prm2.12065","url":null,"abstract":"Inflammation of the thyroid gland is referred to as thyroiditis and is associated with inflammation. Recent studies found significant association between thyroiditis and novel inflammatory and metabolic markers, as well as C‐reactive protein (CRP). CRP/lymphocyte count ratio (CLR) is a novel inflammatory marker that associated with various conditions and has not been studied in thyroiditis, yet. We aimed to investigate CRP to lymphocyte count ratio in patients with thyroiditis and to compare to those in healthy subjects. Patients with thyroiditis that presented to internal medicine outpatient clinics of our institution between January 2019 and August 2021 were enrolled to the retrospective study. Healthy volunteers were enrolled as control group. CLR of the thyroiditis and control groups were compared. Median CLR of the thyroiditis and control groups were 3.14 (0.14%–38)% and 0.4 (0.03–8.86)%, respectively (p < .001). The sensitivity and specificity of CLR > 0.43% in detecting thyroiditis were 92% and 58%, respectively (AUC: 0.88, p < .001, 95% CI: 0.85–0.92).CLR was significantly and positively correlated with free T4 (FT4) (r = .18, p < .001) and inversely correlated with thyroid stimulating hormone (TSH) (r = −.52, p = .003) levels. In conclusion, we suggest that high CLR levels may yield additional diagnostic value in patients with thyroiditis.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"31 - 34"},"PeriodicalIF":0.5,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48232140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elif Basaran, N. Kucukoztas, Halit Oktay Aktepe, B. A. Atak Tel, G. Aktas
Prostate cancer is a common type of malignancy in male population. It may present with various clinical findings including urinary retention. Here, we present a prostate cancer case in an elderly man who investigated for the origin of long‐term fever and weight loss. A 69‐year‐old man presented to our institution for a fever lasting more than a month. He had no other signs of infection. He lost about 15 kg within 3 months. Total prostate‐specific antigen (PSA) measurement in serum and prostate biopsy revealed advanced prostate cancer. His complaints diminished with prostate cancer treatment. In conclusion, we suggest that prostate cancer should be kept in mind in the differential diagnosis of fever and weight loss in men over 50 years of age whether he is symptomatic or not.
{"title":"An exceptional prostate cancer case: Importance of cancer screening","authors":"Elif Basaran, N. Kucukoztas, Halit Oktay Aktepe, B. A. Atak Tel, G. Aktas","doi":"10.1002/prm2.12063","DOIUrl":"https://doi.org/10.1002/prm2.12063","url":null,"abstract":"Prostate cancer is a common type of malignancy in male population. It may present with various clinical findings including urinary retention. Here, we present a prostate cancer case in an elderly man who investigated for the origin of long‐term fever and weight loss. A 69‐year‐old man presented to our institution for a fever lasting more than a month. He had no other signs of infection. He lost about 15 kg within 3 months. Total prostate‐specific antigen (PSA) measurement in serum and prostate biopsy revealed advanced prostate cancer. His complaints diminished with prostate cancer treatment. In conclusion, we suggest that prostate cancer should be kept in mind in the differential diagnosis of fever and weight loss in men over 50 years of age whether he is symptomatic or not.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"39 - 41"},"PeriodicalIF":0.5,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48455828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gambogic acid (GA) is a natural product that selectively induces apoptosis of cancer cells in vitro with high efficiency and low toxicity. Our previous data have shown inhibitory effects of GA on breast cancer cells. However, the detailed mechanisms for GA remain largely unknown. This study aimed to investigate the effect of GA on TGF‐β1‐induced tumor invasion and EMT in MDA‐MB‐231 cells, and contribution of elevated SIRT1 to antitumor effects of GA. Human breast cell MDA‐MB‐231 and MDA‐MB‐231 were incubated with TGF‐β1 (100 ng/ml) and GA. Cell viability was determined by MTT assay, while tumor invasion was determined by Boyden chamber invasion assay. The mRNA levels of SIRT1 and TGF‐β1 were measured by quantitative real‐time polymerase chain reaction (qRT‐PCR). The protein expressions of SIRT1 and EMT‐associated mesenchymal marker Vimentin were measured by Western blotting. Interleukin‐6 (IL‐6) protein content was measured by enzyme linked immunosorbent assays (ELISAs). Our results showed that, GA showed decreased proliferation in MDA‐MB‐231 cells, especially in MDA‐MB‐231 cells with TGF‐β1 incubation. GA inhibited tumor invasion and EMT in TGF‐β1‐treated MDA‐MB‐231 cells. GA increased mRNA and protein expression of SIRT1 in MDA‐MB‐231 cells with and without TGF‐β1 treatment. SIRT1 mRNA level in MDA‐MB‐231 cells was increased by TGF‐β1 incubation and decreased by GA treatment. In addition, the production of IL‐6 was increased by TGF‐β1, and decreased by GA. In conclusion, GA inhibits tumor invasion and EMT in breast cancer, potentially through upregulating SIRT1 expression. This study provides a novel antitumor effect of GA in breast cancer.
{"title":"Gambogic acid inhibits epithelial–mesenchymal transition in breast cancer cells through upregulation of SIRT1 expression in vitro","authors":"Shi‐ye Jiang, Jun Yu, Ming-Ji Zhu, Xiao-mei Zhang, Yuan-ying Zhang, Qin Zhang, Qing Hu, Min-hao Lv","doi":"10.1002/prm2.12057","DOIUrl":"https://doi.org/10.1002/prm2.12057","url":null,"abstract":"Gambogic acid (GA) is a natural product that selectively induces apoptosis of cancer cells in vitro with high efficiency and low toxicity. Our previous data have shown inhibitory effects of GA on breast cancer cells. However, the detailed mechanisms for GA remain largely unknown. This study aimed to investigate the effect of GA on TGF‐β1‐induced tumor invasion and EMT in MDA‐MB‐231 cells, and contribution of elevated SIRT1 to antitumor effects of GA. Human breast cell MDA‐MB‐231 and MDA‐MB‐231 were incubated with TGF‐β1 (100 ng/ml) and GA. Cell viability was determined by MTT assay, while tumor invasion was determined by Boyden chamber invasion assay. The mRNA levels of SIRT1 and TGF‐β1 were measured by quantitative real‐time polymerase chain reaction (qRT‐PCR). The protein expressions of SIRT1 and EMT‐associated mesenchymal marker Vimentin were measured by Western blotting. Interleukin‐6 (IL‐6) protein content was measured by enzyme linked immunosorbent assays (ELISAs). Our results showed that, GA showed decreased proliferation in MDA‐MB‐231 cells, especially in MDA‐MB‐231 cells with TGF‐β1 incubation. GA inhibited tumor invasion and EMT in TGF‐β1‐treated MDA‐MB‐231 cells. GA increased mRNA and protein expression of SIRT1 in MDA‐MB‐231 cells with and without TGF‐β1 treatment. SIRT1 mRNA level in MDA‐MB‐231 cells was increased by TGF‐β1 incubation and decreased by GA treatment. In addition, the production of IL‐6 was increased by TGF‐β1, and decreased by GA. In conclusion, GA inhibits tumor invasion and EMT in breast cancer, potentially through upregulating SIRT1 expression. This study provides a novel antitumor effect of GA in breast cancer.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"14 - 22"},"PeriodicalIF":0.5,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43735994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The count of lymph node dissection (LND) has been confirmed as a prognostic indicator in various cancers while the correlation between LND counts and patient prognosis in renal cell carcinoma (RCC) was not fully studied. This research evaluated the link between LND counts and the prognosis of RCC patients. This study obtained RCC patients from the SEER database between 2010 and 2014. Univariate and multivariate Cox regression analyses were used to assess the predictive and prognostic value of LND. Besides, Kaplan–Meier (KM) survival analysis was used for survival analysis. Finally, 1264 patients were included in this study, which were divided into none LND groups (249), 1–3 LND groups (166), and 4 or more LND groups (549). Age, laterality, stage, M stage, and grade were statistically different among the three groups. In univariate Cox analysis, stage, M stage, T stage, and grade were significantly correlated with overall survival (OS). In multivariate Cox regression analysis, age, race, stage, T stage, and grade were used as independent prognostic factors. KM survival analysis revealed that grade, T stage, M stage, and stage were significantly correlated with OS. Subgroup analysis revealed that LND counts were not significantly associated with survival risk in subgroups with different clinical factors. The current North American population analysis revealed that age, race, stage, T stage, and grade may be used as independent prognostic factors for patients with RCC. Moreover, the increasing LND counts during surgery may not improve the tumor outcomes of RCC patients.
{"title":"The prognostic role of lymph node dissection counts in the management of renal cell carcinoma: A large international cohort study","authors":"Feng Qi, Xiyi Wei, Xin Xia, Zongshi Qin, Xiao Li","doi":"10.1002/prm2.12064","DOIUrl":"https://doi.org/10.1002/prm2.12064","url":null,"abstract":"The count of lymph node dissection (LND) has been confirmed as a prognostic indicator in various cancers while the correlation between LND counts and patient prognosis in renal cell carcinoma (RCC) was not fully studied. This research evaluated the link between LND counts and the prognosis of RCC patients. This study obtained RCC patients from the SEER database between 2010 and 2014. Univariate and multivariate Cox regression analyses were used to assess the predictive and prognostic value of LND. Besides, Kaplan–Meier (KM) survival analysis was used for survival analysis. Finally, 1264 patients were included in this study, which were divided into none LND groups (249), 1–3 LND groups (166), and 4 or more LND groups (549). Age, laterality, stage, M stage, and grade were statistically different among the three groups. In univariate Cox analysis, stage, M stage, T stage, and grade were significantly correlated with overall survival (OS). In multivariate Cox regression analysis, age, race, stage, T stage, and grade were used as independent prognostic factors. KM survival analysis revealed that grade, T stage, M stage, and stage were significantly correlated with OS. Subgroup analysis revealed that LND counts were not significantly associated with survival risk in subgroups with different clinical factors. The current North American population analysis revealed that age, race, stage, T stage, and grade may be used as independent prognostic factors for patients with RCC. Moreover, the increasing LND counts during surgery may not improve the tumor outcomes of RCC patients.","PeriodicalId":40071,"journal":{"name":"Precision Medical Sciences","volume":"11 1","pages":"23 - 30"},"PeriodicalIF":0.5,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46384863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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