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State information system “AITS”: features of formation and directions of development 国家信息系统“AITS”的形成特点及发展方向
IF 0.2 Pub Date : 2021-08-05 DOI: 10.29235/1817-7204-2021-59-3-340-349
I. V. Bryula
Animal breeding is a strategic branch of agri-business in the Republic of Belarus, focused on solving social and economic issues and ensuring national food security. Currently, its development is innovations based, forming a high level of the country’s production and export potential. In the world ranking by the end of 2020, the Republic of Belarus took the 5th place in terms of milk exports (4.8 million tons). As world experience shows, the key direction is implementation of electronic identification of animals as an accounting system in agriculture, including assigning identification number to an animal by tagging, registering information about it in a database and issuing an appropriate passport. The paper summarizes and systematizes legal, organizational and financial conditions for creation and functioning of national information systems in Australia, Argentina, Brazil, Great Britain, the European Union, Canada, the Netherlands, the USA, Switzerland, and Japan. It has been determined that absence of a system for identifying the registration of farm animals leads to distortion of data on the number of livestock, and also creates obstacles for selection work and livestock breeding, decreases efficiency of antiepizootic measures, and inhibits international trade in animals and animal products. In development of this, the necessity of this process in the Republic of Belarus, feasibility of creating the state information system “AITS” (SIS AITS) and corresponding management structure – SI “Center for information systems in animal husbandry” are substantiated. With Gomel region as an example, peculiarities of planning and implementation of process of identification and registration of farm animals are disclosed, the main indicators of the efficiency of SIS AITS for 2013–2020 are analyzed. The advantages of commodity producers of the Republic of Belarus in the context of strengthening production and marketing and export potential and reducing risks in the domestic and foreign markets are substantiated. The issues presented in the article are of interest in determining measures for implementation of the State Program “Agrarian Business” for 2021-2025 and a strategy for the export of agricultural products and food products for the period up to 2025.
动物养殖是白俄罗斯共和国农业经营的一个战略分支,其重点是解决社会和经济问题并确保国家粮食安全。目前,它的发展是以创新为基础的,形成了国家高水平的生产和出口潜力。在2020年底的世界排名中,白俄罗斯共和国在牛奶出口量方面排名第五(480万吨)。世界经验表明,关键方向是实施动物电子识别作为农业会计系统,包括通过标记为动物分配识别号码,在数据库中登记有关动物的信息并颁发适当的护照。本文总结并系统化了澳大利亚、阿根廷、巴西、英国、欧盟、加拿大、荷兰、美国、瑞士和日本建立和运行国家信息系统的法律、组织和财务条件。已经确定,由于缺乏确定农场动物登记的制度,导致牲畜数量数据失真,还对选择工作和牲畜育种造成障碍,降低了抗流行病措施的效率,并抑制了动物和动物产品的国际贸易。在这一发展过程中,白俄罗斯共和国这一进程的必要性,建立国家信息系统“AITS”(SIS AITS)和相应的管理结构- SI“畜牧业信息系统中心”的可行性得到证实。以戈梅利地区为例,揭示了农场动物鉴定登记流程规划与实施的特殊性,分析了2013-2020年SIS AITS效率的主要指标。白俄罗斯共和国商品生产者在加强生产、销售和出口潜力以及减少国内外市场风险方面的优势得到了证实。文章中提出的问题与确定2021-2025年国家“农业企业”计划的实施措施和2025年之前农产品和食品出口战略有关。
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引用次数: 0
Model genetic profiles of pigs of mother breeds on genes - productivity markers 母种猪在基因-生产力标记上的模型遗传谱
IF 0.2 Pub Date : 2021-08-05 DOI: 10.29235/1817-7204-2021-59-3-350-360
O. Vasilyuk, I. Sheiko, I. Gridyushko
Currently, development of molecular genetics and biology allows for genomic analysis and selection directly at the DNA level (marker-dependent selection). The aim of the research was to develop model genetic profiles for marker genes of quantitative traits of productivity of pigs of planned maternal breeds used in pig breeding. Such breeds in the Republic of Belarus are as follows: Belarusian large white, Belarusian black and white and Yorkshire. The studies were carried out at the agricultural branch of Zadneprovsky SGC, Orshansky Plant of Bread Products, JSC, Zarechye SGC, Zapadny SGC with populations of purebred animals of Belarusian Large White, Belarusian Black and White and Belarusian plant type of Yorkshire pigs during 2002-2018. Genetic testing was carried out with sows, boars and finishing pigs of maternal breeds. As a starting material, tissue samples from the auricle of pigs were used with DNA isolated and optimized for the analysis of gene polymorphism using PCR - RFLP method in the laboratories of molecular biotechnology and DNA testing (Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding) and animal genetics (Institute of Genetics and Cytology of the National Academy of Sciences of Belarus). As a result of studies based on the established polymorphism, model genetic profiles of maternal breeds of pigs were developed for marker genes of quantitative traits of productivity. The maximum achieved level of the preferred genotype for each marker gene among the three maternal breeds served as a model profile for the evaluated breeds (breeding young animals). For breeds with an established high level of marker gene polymorphism and productivity, a model profile has been developed that exceeds the achieved indicator by 8-10 p.p.
目前,分子遗传学和生物学的发展允许直接在DNA水平上进行基因组分析和选择(标记依赖选择)。本研究的目的是建立用于猪育种的计划母种猪产量数量性状标记基因的模型遗传谱。这些品种在白俄罗斯共和国如下:白俄罗斯大白,白俄罗斯黑白和约克郡。研究于2002年至2018年期间在扎德涅普罗夫斯基农业股份有限公司、奥尔尚斯基面包生产厂、JSC、扎雷切耶农业股份有限公司、扎帕德尼农业股份有限公司对白俄罗斯大白猪、白俄罗斯黑白猪和白俄罗斯植物型约克郡猪的纯种动物进行。对母种母猪、公猪和育肥猪进行基因检测。以猪耳廓组织样品为起始材料,在分子生物技术和DNA检测实验室(白俄罗斯国家科学院动物育种研究与实践中心)和动物遗传学实验室(白俄罗斯国家科学院遗传与细胞学研究所)分离并优化DNA,采用PCR - RFLP方法进行基因多态性分析。在已建立的多态性研究基础上,建立了猪母种生产力数量性状标记基因的模式遗传谱。在三个母种中,每个标记基因的首选基因型达到的最大水平作为被评估品种(繁殖幼畜)的模型概况。对于已确定具有高水平标记基因多态性和生产力的品种,已开发出超过已实现指标8- 10pp的模型。
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引用次数: 0
Scientific approaches of a multi-level competitiveness assessment of quality assurance system for AIC products AIC产品质量保证体系多层次竞争力评价的科学方法
IF 0.2 Pub Date : 2021-08-05 DOI: 10.29235/1817-7204-2021-59-3-263-276
P. Rastorgouev
High dependence of agri-food products competitiveness level on its qualitative parameters determines the need to orientate both the management of individual enterprises and the entire APC regulation system for formation of competitive advantages of domestic products in order to efficiently implement it in external and domestic markets. A prerequisite for reliably determine the direction of efficient solution to solve such a task is availability of a mechanism for objective assessment of the current product quality management system from the point of view of providing sustainable production of competitive product according to quality parameters. In connection with these objectives of the study were substantiation and development of practical oriented scientific approaches to assessment of competitiveness of the system for ensuring quality of agri-food products in modern conditions, taking into account the multi-levelness of the APC regulation system in the Republic of Belarus. As a result, system of principles of assessing competitiveness of system for ensuring quality of agri-food products has been developed. Targeted estimated criteria specifying the level of competitiveness of the system for ensuring quality differentially according to AIC regulation levels have been specified (republican, sectoral, individual business entities). Priority estimates of competitiveness of the quality assurance system for agri-food products have been developed, classified depending on the criteria of quality and safety assurance system, as well as the level of regulation of AIC and their area of application (national, regional and international markets). Implementation of proposals for assessing the competitiveness of the product quality assurance system at all levels of AIC management in practical activities will allow substantiating the most relevant areas for improving the existing quality management mechanisms, increase competitiveness of products in terms of quality parameters and strengthen the position of domestic manufacturers by increasing the confidence of potential and actual consumers in the supply system quality and safety of agri-food products.
农产品竞争力水平对其质量参数的高度依赖,决定了需要对单个企业的管理和整个APC监管体系进行定位,以形成国内产品的竞争优势,以便在国内外市场上有效地实施。可靠地确定解决这一任务的有效解决方案的方向的先决条件是,从根据质量参数提供有竞争力的产品的可持续生产的角度出发,提供一种对当前产品质量管理体系进行客观评估的机制。与这些研究目标有关的是,考虑到白俄罗斯共和国APC管理制度的多层次,确定和发展面向实际的科学方法,以评估在现代条件下确保农产品质量的制度的竞争力。因此,形成了农产品质量保障体系竞争力评价原则体系。已经指定了有针对性的估计标准,指定了系统的竞争力水平,以确保根据AIC监管水平不同的质量(共和国,部门,个体商业实体)。根据质量和安全保证体系的标准以及AIC的管理水平及其应用领域(国家、区域和国际市场),制定了对农产品质量保证体系竞争力的优先估计,并进行了分类。在实际活动中执行评估行政管理各级产品质量保证制度的竞争力的建议,将使改进现有质量管理机制的最相关领域具体化;提高产品在质量参数方面的竞争力,并通过提高潜在和实际消费者对农产品供应系统质量和安全的信心,加强国内制造商的地位。
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引用次数: 0
Impact of lactic acid product on quality indices of raw meat for the smoked sausages production 乳酸产品对烟熏香肠原料肉质指标的影响
IF 0.2 Pub Date : 2021-08-05 DOI: 10.29235/1817-7204-2021-59-3-378-384
S. Merzlov, N. Fedoruk, A. Andriichuk, Y. Fedoruk, V. N. Nadtochii, O. Hrebelnyk, H. Kalinina, S. Polishchuk
Conducted research pertains to technologies of animal products processing and the development of biotechnological methods for stabilizing microbial and enzymatic biological leaven for meat.Materials and Methods. The studies were conducted in the conditions of the Research Institute of Food and Animal Processing Technologies and Laboratory of Microbiological Research Methods, Department of Microbiology of Bila Tserkva National Agrarian University. Moisture retaining capacity and bacterial contamination of beef and pork were determined.Results. The effect of processing raw meat with lactic acid product – yogurt on the moisture retaining capacity was experimentally established. With the highest titratable acidity of yoghurt of 100-110 °T, the moisture retaining capacity of pork and beef was inferior to control on 2.13 and 1.29 % and amounted to 51.88 and 62.73 %. Under a titratable acidity of lactic acid beverage of 100-110 °T with total number of lactic acid bacteria on the surface of raw meat, it was 8.1×108 and 8.5×108, which prevailed samples with acidity level of 60-70 °T on 24.5 and 24.3 %.Conclusions. The results of studies have revealed the effect of processing raw meat by yogurt with different titratable acidity on moisture retaining capacity and bacterial contamination.
所进行的研究涉及动物产品加工技术和生物技术方法的发展,以稳定肉类的微生物和酶生物发酵。材料与方法。研究在Bila Tserkva国立农业大学微生物系食品和动物加工技术研究所和微生物研究方法实验室进行。测定了牛肉和猪肉的保湿性能和细菌污染情况。通过实验确定了乳酸产品酸奶对生肉保水性能的影响。酸奶的最高可滴定酸度为100 ~ 110°T,猪肉和牛肉的保湿率分别为2.13%和1.29%,低于对照,分别为51.88%和62.73%。乳酸饮料可滴定酸度为100-110°T时,原料肉品表面乳酸菌总数分别为8.1×108和8.5×108,酸度为60-70°T时,乳酸菌总数分别占24.5%和24.3%。研究结果揭示了不同可滴定酸度的酸奶加工生肉对其保湿能力和细菌污染的影响。
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引用次数: 0
Mathematical model of agricultural crop yield 农作物产量的数学模型
IF 0.2 Pub Date : 2021-08-05 DOI: 10.29235/1817-7204-2021-59-3-304-318
A. Likhatsevich
Theoretical basis for presenting research results in agricultural science is mathematical statistics and probability theory using empirical forms of generalization of experimental data. To improve the methods of planning field experiment and processing its data using digital technologies, we proposed to use mathematical modeling based on physical principle of balance of cause-and-effect interactions in a closed physical system as a priority option. When analyzing impact of environmental factors on crop yields, the initial provisions, the mathematical modeling of the crop yield is based, on are not associated with characteristics of crops and natural conditions, therefore, the model options are universal in application and are valid for any agricultural crop, regardless of the region of cultivation. To ensure statistically correct digital information, based on the established forms of mathematical model, the field experiment layout aimed at establishing the dependence of the crop yield on yield-forming factors should include at least 4 options for nutritional levels (NPK) with a research duration of at least 4 years. To check the accuracy of the developed crop yield model, the data of independent field experiments of Professor N.N. Semenenko with barley and winter triticale has been used. It has been determined that, in Belarus, yield-forming factors, as a result of their impact on the grain yield, are arranged in the following decreasing sequence: total dose of applied NPK º the amount of precipitation during the active phases of growing season → air temperature for the same period. Calculations have shown that decrease in the number of yield-forming factors taken into account in the mathematical model from three (food, moisture and heat) to two (food and moisture) reduces the accuracy of calculating the grain crop yield insignificantly.
农业科学研究成果呈现的理论基础是数理统计和概率论,运用实验数据推广的经验形式。为了改进利用数字技术规划野外实验和处理实验数据的方法,我们建议优先采用基于封闭物理系统中因果相互作用平衡的物理原理的数学建模。在分析环境因素对作物产量的影响时,最初规定的作物产量的数学建模是基于与作物特性和自然条件无关的,因此,模型选项在应用中是通用的,并且对任何农业作物都有效,无论种植区域如何。为保证数字信息的统计准确性,在建立数学模型的基础上,旨在确定作物产量对产量形成因素的依赖关系的田间试验布局应至少包括4个营养水平(NPK)选项,研究时间至少为4年。为了验证所建立的作物产量模型的准确性,采用了N.N. Semenenko教授对大麦和冬季小黑麦进行的独立田间试验数据。据确定,在白俄罗斯,由于影响粮食产量,形成产量的因素按以下降序排列:施用氮磷钾的总剂量º生长季节活跃阶段的降水量→同一时期的气温。计算表明,数学模型中考虑的产量形成因素从3个(粮食、水分和热量)减少到2个(粮食和水分),对粮食作物产量计算的准确性降低不显著。
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引用次数: 2
Analysis of institutional arrangements of financial institutions in the national economy of Belarus 白俄罗斯国民经济中金融机构的制度安排分析
IF 0.2 Pub Date : 2021-02-09 DOI: 10.29235/1817-7204-2021-59-1-7-21
Y. V. Gruzinskaya
Theoretical and methodological foundations of institutional policy define reduction of uncertainty in activities of organizations and individuals as the main task of financial institutions by establishing stable mechanisms of interaction between participants in public relations. Coordination of economic interests at all levels is performed by both formal and informal institutions, while creation and modification of formal institutions largely depends on the informal influence of representatives of various social macrogroups. The list of the most important macroeconomic institutions includes: institutions of macroeconomic regulation; market institutions; monetary institutions, fiscal institutions, investment institutions; institutions of law; institutions for regulation of foreign economic activity. Whereas, the importance and peculiarities of financial policy in the institutional system determine the special importance of financial institutions, primarily in implementation of measures aimed at ensuring sustainable social and economic development. The paper dwells on practice of functioning of the financial system of Belarus. The value of a unique mega-regulator for financial transactions, exchanging assets and risks has been determined. It has been substantiated that creation of such a supranational body is possible subject to formation of an integrated financial market for the EAEU member states. Impact of the main instruments of financial policy (Belarusian ruble exchange rate, refinancing rate, money supply, required reserves of second-tier banks, monetary policy stability, informal influence measures, agreements, transactions, open market operations, tariffs, duties, restrictions, decrees, orders, guarantees) on the national economy has been considered, proposals for improving financial instruments within the framework of the current institutional mechanism has been substantiated. Use of the proposed results and proposals in practice for improving institutional mechanisms, including a range of official regulations and informal institutions, will promote improvement of the situation with the balance of payments, assessment of fiscal risks and problem assets.
制度政策的理论和方法基础将减少组织和个人活动中的不确定性定义为金融机构的主要任务,方法是在公共关系参与者之间建立稳定的互动机制。各级经济利益的协调由正式机构和非正式机构共同完成,而正式机构的创建和修改在很大程度上取决于各种社会宏观群体代表的非正式影响。最重要的宏观经济机构包括:宏观经济调控机构;市场机构;金融机构、财政机构、投资机构;法律机构;管理对外经济活动的机构。鉴于金融政策在制度体系中的重要性和特殊性决定了金融机构的特殊重要性,主要是在实施旨在确保可持续社会和经济发展的措施方面。本文论述了白俄罗斯金融体系运作的实践。一个独特的巨型监管机构对金融交易、交换资产和风险的价值已经确定。事实证明,建立这样一个超国家机构是可能的,前提是形成一个欧亚经济联盟成员国的综合金融市场。审议了主要金融政策工具(白俄罗斯卢布汇率、再融资利率、货币供应、二级银行法定准备金、货币政策稳定性、非正式影响措施、协议、交易、公开市场业务、关税、关税、限制、法令、命令、担保)对国民经济的影响,并提出了在现行体制机制框架内改进金融工具的建议。在实践中利用所提出的结果和建议来改进体制机制,包括一系列官方条例和非正式机构,将有助于改善国际收支、评估财政风险和问题资产的情况。
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引用次数: 1
Peculiarities of sheep pathogenesis at associative invasions of gastrointestinal tract 绵羊胃肠道联合侵袭发病机制的特点
IF 0.2 Pub Date : 2021-02-09 DOI: 10.29235/1817-7204-2021-59-1-81-89
A. N. Dudarchuk
In the Republic of Belarus, primarily in farms with imperfect technology, parasitic diseases of sheep are widespread, including: nematodes of gastrointestinal tract, strongyloidosis, eimeriosis, monieziosis, trichocephalosis, cryptosporidiosis, etc. Study of impact of associative parasitosis of sheep on the immunobiological reactivity of animal body has recently become more and more theoretical and practical for rational use of drugs in treatment of these diseases. The purpose of research is to study peculiarities of sheep pathogenesis at associative invasions of gastrointestinal tract. The study was carried out at farm “Villia-agro”, Kobrin district, Brest region. Lambs of 2-4 months of age spontaneously infested with parasites of gastrointestinal tract have been selected. Parameters of cellular immunity were determined: number of leukocytes, monocytes, neutrophils, eosinophils, level of rosette-forming T- and B-lymphocytes and humoral immunity: circulating immune complexes, total protein, protein fractions, including proteins of C3 complement system and immunoglobulins, macro and microelements (calcium, phosphorus and iron) in blood serum. With spontaneous invasion of sheep by associations of parasites of gastrointestinal tract, the following changes have been determined: significant decrease in number of lymphocytes, T-lymphocytes, concentration of total protein, albumin, - β- and g-globulins, calcium and phosphorus. Significant increase in leukocytes: Eosinophils and stab neutrophils, a1-globulins , circulating immune complexes. All this together indicates disturbance in functioning of body’s immunity and requires immediate appropriate treatment aimed both at destroying associations of parasites of gastrointestinal tract and restoring immune system of animal’s body. These studies will form basis for development of rational system for therapeutic and preventive measures for associative parasitosis of sheep in the Republic of Belarus, which will reduce economic damage from these diseases and improve quality of livestock products. 
在白俄罗斯共和国,主要是在技术不完善的农场,绵羊的寄生虫病很普遍,包括:胃肠道线虫病、类圆线虫病、艾美拉虫病、莫奈虫病、头毛蚴病、隐孢子虫病等。研究绵羊伴生寄生虫病对动物机体免疫生物学反应性的影响,对合理用药治疗具有重要的理论意义和现实意义。本研究的目的是研究绵羊胃肠道联合侵袭的发病特点。该研究在布雷斯特地区科布林地区的“Villia-agro”农场进行。选择2-4月龄自发感染胃肠道寄生虫的羔羊。测定细胞免疫参数:白细胞、单核细胞、中性粒细胞、嗜酸性粒细胞数量、形成玫瑰花的T淋巴细胞和b淋巴细胞水平、体液免疫:循环免疫复合物、总蛋白、蛋白组分(包括C3补体系统蛋白和免疫球蛋白)、血清宏量元素和微量元素(钙、磷、铁)。随着胃肠道寄生虫的自发入侵,绵羊的淋巴细胞、t淋巴细胞数量显著减少,总蛋白、白蛋白、β-和g-球蛋白、钙和磷的浓度显著降低。白细胞显著增加:嗜酸性粒细胞和中性粒细胞,a1-球蛋白,循环免疫复合物。所有这些都表明机体免疫功能紊乱,需要立即进行适当的治疗,旨在破坏胃肠道寄生虫的联系,恢复动物机体的免疫系统。这些研究将为制定白俄罗斯共和国绵羊伴生寄生虫病的合理治疗和预防措施体系奠定基础,从而减少这些疾病造成的经济损失并提高畜产品质量。
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引用次数: 0
Economic and mathematical model of bee colonies housing in two-block hive with combined technological partitionEconomic and mathematical model of bee colonies housing in two-block hive with combined technological partition 联合技术分区双块蜂箱中蜂群居住的经济与数学模型联合技术分区双块蜂箱中蜂群居住的经济与数学模型
IF 0.2 Pub Date : 2021-02-09 DOI: 10.29235/1817-7204-2021-59-1-103-110
V. Petrovets, T. L. Khromenkova, L. A. Shershneva, A. N. Shershnev
One of the main directions of increasing the economic efficiency of bee products is increase in productivity of bee colonies with economical expenditure of material resources, primarily use of such basic means as new design bee hive. A completely new design of a two-block bee hive is proposed, allowing two families of bees in one house. The two-block bee hive allows to use a simple technological partition to combine two different families with their uterus and get one super-strong family. In two-block hives, the main drawback of one-block hives has been eliminated – significant weight of hive body, which restrains increase in productivity of beekeeper and prevents decrease in product cost price. The volume of a two-block bee hive and the parameters of a combined technological partition, which were obtained as a result of observations and longterm experiments, have been justified. Economic and mathematical model of bee colonies development when housed in two-block hive with combined technological partition is presented. Practical calculation of the economic potential of two-block bee hive has been made. Substantiation of regularity of obtaining high results in housing and peaceful unification of bee colonies in a two-block bee hive with a technological combined partition is given. Keeping bees in the developed two-block hives opens up great prospects in the field of beekeeping.
提高蜂产品经济效益的主要方向之一是在节约物质资源的前提下提高蜂群的生产力,主要是采用新设计蜂箱等基本手段。提出了一种全新的双街区蜂箱设计,允许两个家庭的蜜蜂在一个房子里。双块蜂箱允许使用一个简单的技术隔板将两个不同的家庭与子宫结合在一起,形成一个超级强大的家庭。在双块蜂箱中,消除了单块蜂箱的主要缺点-蜂箱体重量大,限制了养蜂人生产力的提高,阻碍了产品成本价格的下降。通过观察和长期实验,确定了双块蜂窝的体积和组合工艺分区的参数。提出了双块蜂箱组合技术分区下蜂群发展的经济模型和数学模型。对双块蜂窝的经济潜力进行了实际计算。论证了采用技术组合分割的双块蜂箱在围护和蜂群和平统一方面取得较高效果的规律性。在发达的双块蜂箱中养蜂,在养蜂领域开辟了广阔的前景。
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引用次数: 0
Genome-wide association study (GWAS) with productivity in Romanov sheep breed 罗曼诺夫羊品种生产能力与全基因组关联研究
IF 0.2 Pub Date : 2021-02-09 DOI: 10.29235/1817-7204-2021-59-1-71-80
A. Krivoruchko, O. Yatsyk, T. Saprikina, D. D. Petukhova
Genetic technologies used in breeding of small ruminants requires searching for new molecular markers of productive traits. The most effective for this is genome-wide association study (GWAS) of single nucleotide polymorphisms (SNP) with economically valuable traits. The paper presents results of study of associations of the frequency of single nucleotide polymorphisms with a rank assessment according to complex of productive traits (super-elite) in Romanov sheep using DNA biochips Ovine Infinium HD BeadChip 600K. Eleven SNPs have been found having significant correlation with the animals belonging to the “super-elite” group. Five substitutions are located in the genes introns, six are related to intergenic polymorphisms. The highest reliability of association with productivity was observed in substitution rs410516628 (р = 3,14 · 10-9) located on the 3rd chromosome. Substitution rs422028000 on 2nd chromosome differs with the fact that in the “super-elite” group it was found in 90 % of haplotypes. Polymorphisms rs411162754 (1st chromosome) and rs417281100 (10th chromosome) in our study turned out to be the rarest – only in “super-elite” group and only in a quarter of haplotypes. The genes located near the identified SNPs are mainly associated with metabolic and regulatory processes. Our study has identified several new candidate genes with polymorphism probably associated with the ranking in terms of productivity in Romanov sheep: LTBP1, KCNH8, LMX1B, ZBTB43, MSRA, CHPF, PID1 and DNER. The results obtained create a theoretical basis for further study of candidate genes affecting implementation of phenotypic traits in Romanov sheep. The revealed polymorphisms associated with the productive traits of sheep can be used in practical breeding as molecular and genetic markers for selection of parental pairs.
在小型反刍动物育种中应用遗传技术需要寻找新的生产性状分子标记。最有效的方法是对具有经济价值性状的单核苷酸多态性(SNP)进行全基因组关联研究。本文介绍了利用Ovine Infinium HD BeadChip 600K DNA生物芯片研究罗曼诺夫羊单核苷酸多态性频率与生产性状复合体(超精英)等级评价的关系。11个snp被发现与属于“超级精英”群体的动物有显著的相关性。5个替换位点位于基因内含子上,6个与基因间多态性有关。在位于第3染色体上的rs410516628 (r = 3,14·10-9)中,与生产力相关的可靠性最高。在“超级精英”群体中,90%的单倍型都存在rs422028000替代,这与第2染色体上的rs422028000替代不同。在我们的研究中,rs411162754(第1染色体)和rs417281100(第10染色体)的多态性是最罕见的,仅在“超级精英”群体中,仅在四分之一的单倍型中存在。位于鉴定snp附近的基因主要与代谢和调节过程相关。我们的研究发现了几个新的候选基因,它们的多态性可能与罗曼诺夫羊的生产力排名有关:LTBP1, KCNH8, LMX1B, ZBTB43, MSRA, CHPF, PID1和DNER。研究结果为进一步研究影响罗曼诺夫羊表型性状实现的候选基因奠定了理论基础。所揭示的与绵羊生产性状相关的多态性可作为实际育种中亲本选择的分子和遗传标记。
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引用次数: 0
Polymorphism of wild species of р. Malus MILL. according to powdery mildew resistance genes 野生种的多态分析。马吕斯。根据白粉病抗性基因
IF 0.2 Pub Date : 2021-02-09 DOI: 10.29235/1817-7204-2021-59-1-62-70
A. Lyzhin, N. Savel’eva
Powdery mildew (Podosphaera leucotricha (Ell. E tEv.) Salm.) – is one of the most widespread apple diseases in world. Identification of forms, carrying resistance genes, is an important stage in breeding programs aimed at obtaining powdery mildew resistant apple varieties. Diagnostic DNA markers of target genes will increase reliability of identification and efficiency of apple breeding for the creation of resistant genotypes. The purpose of this study was molecular genetic testing of wild species of genus Malus Mill. According to Pl-1, Pl-w and Pl-d powdery mildew resistance genes for revealing polymorphism of the studied loci and identification of valuable genotypes for breeding. The study subjects were the wild species of genus Malus Mill. of different ecological and geographic origin. The Pl-1 gene was identified using AT20-SCAR marker, Pl-w gene - EM M02 marker, Pl-d gene - EM DM01 marker. AT20-SCAR marker (Pl-1 gene) was identified in 37.3 % of genotypes. EM M02 marker (Pl-w gene) was detected in 16.4 % of the studied forms. EM DM01 marker (Pl-d gene) is present in 10.4 % of the analyzed forms. At least one of the studied molecular markers is present in the genome of 52.2 % of apple wild species. The Pl-1 gene in apple wild species is most spread in Baccatae series, the Pl-w gene - in Baccatae and Sieboldinae series, the Pl-d gene - in Orientalis series. Wild species M. baccata 2319, M. mandshurica 41947, M. sachalinensis 85, M. sachalinensis 97, M. purpurea v. pendula 2396 are characterized by combination of Pl-1 and Pl-w genes; M. turkmenorum 13283 and M. turkmenorum 29 421 - Pl-1 and Pl-d genes; M. denticulata 29416 - Pl-w and Pl-d genes, which allows to recommend them as promising complex sources of high powdery mildew resistance for breeding.
白粉病(Podosphaera leucotricha)E tEv)。是世界上最普遍的苹果病之一。携带抗性基因的品种鉴定是获得抗白粉病苹果品种育种计划的重要阶段。目标基因的诊断性DNA标记将提高苹果抗性基因型鉴定的可靠性和育种效率。本研究的目的是对海棠属野生种进行分子遗传检测。根据Pl-1、Pl-w和Pl-d白粉病抗性基因,揭示所研究位点的多态性,鉴定有育种价值的基因型。以海棠属野生种为研究对象。不同生态和地理起源的。采用AT20-SCAR标记、Pl-w基因- EM M02标记、Pl-d基因- EM DM01标记对Pl-1基因进行鉴定。37.3%的基因型检测到AT20-SCAR标记(Pl-1基因)。在16.4%的研究品种中检测到EM M02标记(Pl-w基因)。emdm01标记(Pl-d基因)存在于10.4%的分析形式中。研究的分子标记至少有一个存在于52.2%的苹果野生物种的基因组中。苹果野生种中Pl-1基因在Baccatae系列中传播最多,Pl-w基因-在Baccatae和Sieboldinae系列中传播最多,Pl-d基因-在Orientalis系列中传播最多。野生种M. baccata 2319、M. mandshurica 41947、M. sachalinensis 85、M. sachalinensis 97、M. purpurea v. pendula 2396以Pl-1和Pl-w基因组合为特征;土株13283和土株29 421 - Pl-1和Pl-d基因;M. denticulata 29416 - Pl-w和Pl-d基因,可以推荐它们作为高白粉病抗性的复杂来源进行育种。
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引用次数: 2
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Proceedings of the National Academy of Sciences of Belarus-Agrarian Series
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