Journal of Agricultural and Food Chemistry最新文献

英文中文

Scientific Evidence Challenges the EU Residue Definition Established for the Control of Ethylene Oxide in Food 科学证据质疑欧盟为控制食品中环氧乙烷残留而建立的定义

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-06 DOI: 10.1021/acs.jafc.5c13149

Thomas Bessaire, Thierry Delatour

Five years after the initial recalls of various food products exceeding ethylene oxide (ETO) limits in the European Union, alerts keep being regularly issued, highlighting the continuous need for monitoring food items imported in Europe. However, the reliability of ETO measurement remains often questionable, mainly due to its high reactivity and volatility, ultimately complicating residue interpretation. This study summarizes the existing chemical and biochemical evidence on ETO and its major metabolite, 2-chloroethanol, while pointing out the regulatory ambiguities and challenges associated with reliable measurement and analytical method standardization.

在欧盟首次召回各种超过环氧乙烷（ETO）限制的食品五年后，警报仍在定期发布，突显了持续监测欧洲进口食品的必要性。然而，ETO测量的可靠性仍然经常受到质疑，主要是由于其高反应性和挥发性，最终使残留物解释复杂化。本研究总结了现有的关于ETO及其主要代谢物2-氯乙醇的化学和生化证据，同时指出了与可靠的测量和分析方法标准化相关的监管歧义和挑战。

引用次数: 0

Paenibacillus polymyxa K17 Controls Leaf Spot of Tea Plant by Impairing Fungal Cellular Structure and Energy Metabolism 多粘类芽孢杆菌K17通过破坏真菌细胞结构和能量代谢控制茶树叶斑病

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-06 DOI: 10.1021/acs.jafc.5c14073

Changfei Yang, Yanyu Chen, Yubo Sun, Wishwajith Kandegama, Jie Yin

Utilizing biocontrol microbes has emerged as a promising strategy for sustainable disease management in tea cultivation. Here, a novel strain Paenibacillus polymyxa K17 was isolated, which possesses broad-spectrum antifungal capacity against 14 phytopathogens from multiple crops, with inhibition rates of 53–82%. Notably, P. polymyxa K17 demonstrated comparable or occasionally superior control efficacy to the fungicide kasugamycin in leaf spot disease caused by Lasiodiplodia gonubiensis across three tea cultivars. P. polymyxa K17 induced mycelial deformation and cellular damage of L. gonubiensis. Meanwhile, strain K17 metabolites markedly suppressed the gene expression in multiple pathways related to fungal energy metabolism, including oxidative phosphorylation, TCA cycle, and pyruvate metabolism. Correspondingly, the activities of the mitochondrial complex and the levels of ATP and pyruvate in L. gonubiensis were markedly decreased. These findings indicate that P. polymyxa K17 inhibits fungal growth by disrupting energy metabolism and cellular structure, highlighting its potential for controlling multiple fungal diseases in tea gardens.

利用生物防治微生物已成为茶叶可持续病害管理的一种有前景的策略。本研究分离到一株多粘类芽孢杆菌K17，该菌株对来自多种作物的14种植物病原菌具有广谱抗真菌能力，抑菌率为53 ~ 82%。值得注意的是，P. polymyxa K17在3个茶叶品种中对gonubiensis Lasiodiplodia叶斑病的防治效果与杀菌剂kasugamycin相当，有时甚至更好。P. polymyxa K17诱导水蛭菌丝变形和细胞损伤。同时，菌株K17代谢物显著抑制真菌能量代谢相关的多种途径的基因表达，包括氧化磷酸化、TCA循环和丙酮酸代谢。相应的，谷奴螺旋藻线粒体复合体活性、ATP和丙酮酸水平明显降低。这些结果表明，P. polymyxa K17通过破坏能量代谢和细胞结构抑制真菌生长，突出了其控制茶园多种真菌病害的潜力。

{"title":"Paenibacillus polymyxa K17 Controls Leaf Spot of Tea Plant by Impairing Fungal Cellular Structure and Energy Metabolism","authors":"Changfei Yang, Yanyu Chen, Yubo Sun, Wishwajith Kandegama, Jie Yin","doi":"10.1021/acs.jafc.5c14073","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c14073","url":null,"abstract":"Utilizing biocontrol microbes has emerged as a promising strategy for sustainable disease management in tea cultivation. Here, a novel strain Paenibacillus polymyxa K17 was isolated, which possesses broad-spectrum antifungal capacity against 14 phytopathogens from multiple crops, with inhibition rates of 53–82%. Notably, P. polymyxa K17 demonstrated comparable or occasionally superior control efficacy to the fungicide kasugamycin in leaf spot disease caused by Lasiodiplodia gonubiensis across three tea cultivars. P. polymyxa K17 induced mycelial deformation and cellular damage of L. gonubiensis. Meanwhile, strain K17 metabolites markedly suppressed the gene expression in multiple pathways related to fungal energy metabolism, including oxidative phosphorylation, TCA cycle, and pyruvate metabolism. Correspondingly, the activities of the mitochondrial complex and the levels of ATP and pyruvate in L. gonubiensis were markedly decreased. These findings indicate that P. polymyxa K17 inhibits fungal growth by disrupting energy metabolism and cellular structure, highlighting its potential for controlling multiple fungal diseases in tea gardens.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"177 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth-Coupled Screening Enables Efficient Engineering of Key Enzymes for Chlorogenic Acid Biosynthesis 生长偶联筛选实现绿原酸生物合成关键酶的高效工程

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.5c14119

Yueting Zeng, La Xiang, Shizhong Li, Jian-Ming Jin, Chaoning Liang, Shuang-Yan Tang

Chlorogenic acid (CGA) is a vital phenolic ester with extensive applications in the food, pharmaceutical, and cosmetic industries. Although numerous microbial cell factories have been developed for CGA biosynthesis, the low catalytic efficiency of the key enzyme hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) remains a major bottleneck for high-yield production. In this study, we developed a novel growth-coupled selection system based on HQT-mediated reduction of toxic caffeoyl-CoA accumulation, thereby relieving growth inhibition in engineered Escherichia coli. Through iterative rounds of directed evolution, we identified a high-performance HQT variant enhancing CGA production by 3.7-fold compared with the wild-type enzyme. The specific activity was improved by 1.8-fold. Structural analysis of the mutant revealed critical insights into the functional role of the crossover loop in modulating enzymatic activity, offering new perspectives for HQT rational engineering. This work provides both a mechanistic understanding and a practical framework for enhancing CGA biosynthesis by addressing key enzyme bottlenecks.

绿原酸（CGA）是一种重要的酚类酯，在食品、制药和化妆品工业中有着广泛的应用。尽管已经开发了许多用于CGA生物合成的微生物细胞工厂，但关键酶羟基肉桂酰辅酶a醌酸羟基肉桂酰转移酶（HQT）的低催化效率仍然是高产产的主要瓶颈。在这项研究中，我们开发了一种新的生长偶联选择系统，该系统基于hqt介导的毒性咖啡酰辅酶a积累的减少，从而减轻了工程大肠杆菌的生长抑制。通过反复的定向进化，我们发现了一个高性能的HQT变体，与野生型酶相比，CGA的产量提高了3.7倍。比活性提高1.8倍。突变体的结构分析揭示了交叉环在调节酶活性中的功能作用，为HQT合理工程提供了新的视角。这项工作为通过解决关键酶瓶颈来增强CGA生物合成提供了机制理解和实践框架。

{"title":"Growth-Coupled Screening Enables Efficient Engineering of Key Enzymes for Chlorogenic Acid Biosynthesis","authors":"Yueting Zeng, La Xiang, Shizhong Li, Jian-Ming Jin, Chaoning Liang, Shuang-Yan Tang","doi":"10.1021/acs.jafc.5c14119","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c14119","url":null,"abstract":"Chlorogenic acid (CGA) is a vital phenolic ester with extensive applications in the food, pharmaceutical, and cosmetic industries. Although numerous microbial cell factories have been developed for CGA biosynthesis, the low catalytic efficiency of the key enzyme hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) remains a major bottleneck for high-yield production. In this study, we developed a novel growth-coupled selection system based on HQT-mediated reduction of toxic caffeoyl-CoA accumulation, thereby relieving growth inhibition in engineered Escherichia coli. Through iterative rounds of directed evolution, we identified a high-performance HQT variant enhancing CGA production by 3.7-fold compared with the wild-type enzyme. The specific activity was improved by 1.8-fold. Structural analysis of the mutant revealed critical insights into the functional role of the crossover loop in modulating enzymatic activity, offering new perspectives for HQT rational engineering. This work provides both a mechanistic understanding and a practical framework for enhancing CGA biosynthesis by addressing key enzyme bottlenecks.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"72 3-4 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146116227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Insights into Soy Protein–Lupin Flour Meat Analogues through FTIR Spectrochemical Analysis 通过FTIR光谱化学分析大豆蛋白-罗苹面粉肉类类似物的分子洞察

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.5c10733

Aayushi Kadam, Matias Rodriguez Elhordoy, Daniel Vazquez, Alejandra Medrano, Kathleen M. Gough, Filiz Koksel

Fourier transform infrared (FTIR) spectroscopy and spectrochemical imaging were used to investigate the structural and compositional changes in extruded high-moisture meat analogues (HMMAs) produced from soy protein isolate and lupin flour (LF) blends. Extrusion induced shifts in the amide I band (∼1637 cm^–1 in raw blends to ∼1650 cm^–1 in HMMAs), indicating protein denaturation and structural rearrangement associated with fibrous structure formation. Changes in carbohydrate- and lipid-associated bands further reflected extrusion-driven molecular reorganization. FTIR spectrochemical analysis revealed distinct HMMA regions, including aligned protein-rich domains and lipid–starch-rich domains appearing as polygonal grids. Increasing the LF content from 15 to 45% progressively increased lipid- and carbohydrate-associated spectral features (i.e., areas of the fatty acid ester peak at 1744 cm^–1 and the carbohydrate peak around 1060 cm^–1) relative to proteins. These insights advance the understanding of structure–function relationships in HMMAs and highlight the value of FTIR spectrochemical imaging for optimizing texture and the effective design of meat analogues.

采用傅里叶变换红外光谱（FTIR）和光谱化学成像技术研究了大豆分离蛋白与露平粉（LF）共混物挤压制高水分肉类类似物（HMMAs）的结构和成分变化。挤压诱导了酰胺I带的移位（原料共混物中的~ 1637 cm-1到hmma中的~ 1650 cm-1），表明蛋白质变性和结构重排与纤维结构的形成有关。碳水化合物和脂质相关条带的变化进一步反映了挤压驱动的分子重组。FTIR光谱化学分析显示了不同的HMMA区域，包括排列的富含蛋白质的区域和富含脂质淀粉的区域，呈多边形网格状。将LF含量从15%增加到45%，相对于蛋白质，脂质和碳水化合物相关的光谱特征（即脂肪酸酯峰在1744 cm-1处的面积和碳水化合物峰在1060 cm-1左右的面积）逐渐增加。这些见解促进了对hmma结构-功能关系的理解，并突出了FTIR光谱化学成像在优化质地和有效设计肉类类似物方面的价值。

{"title":"Molecular Insights into Soy Protein–Lupin Flour Meat Analogues through FTIR Spectrochemical Analysis","authors":"Aayushi Kadam, Matias Rodriguez Elhordoy, Daniel Vazquez, Alejandra Medrano, Kathleen M. Gough, Filiz Koksel","doi":"10.1021/acs.jafc.5c10733","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c10733","url":null,"abstract":"Fourier transform infrared (FTIR) spectroscopy and spectrochemical imaging were used to investigate the structural and compositional changes in extruded high-moisture meat analogues (HMMAs) produced from soy protein isolate and lupin flour (LF) blends. Extrusion induced shifts in the amide I band (∼1637 cm–1 in raw blends to ∼1650 cm–1 in HMMAs), indicating protein denaturation and structural rearrangement associated with fibrous structure formation. Changes in carbohydrate- and lipid-associated bands further reflected extrusion-driven molecular reorganization. FTIR spectrochemical analysis revealed distinct HMMA regions, including aligned protein-rich domains and lipid–starch-rich domains appearing as polygonal grids. Increasing the LF content from 15 to 45% progressively increased lipid- and carbohydrate-associated spectral features (i.e., areas of the fatty acid ester peak at 1744 cm–1 and the carbohydrate peak around 1060 cm–1) relative to proteins. These insights advance the understanding of structure–function relationships in HMMAs and highlight the value of FTIR spectrochemical imaging for optimizing texture and the effective design of meat analogues.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146116226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenol-Loaded Plant Extracellular Vesicles: A New Approach to Combat AGEs-Induced Neurotoxicity via the Microbiota-Gut-Brain Axis 多酚负载植物细胞外囊泡：通过微生物-肠-脑轴对抗年龄诱导的神经毒性的新方法

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.5c08992

Ying Cao, Rui Wang, Zihan Ni, Ting Luo, Xin Zhang

Excessive dietary intake of advanced glycation end products (AGEs) is a potential risk factor for neurodegenerative diseases (NDDs). The underlying mechanism may involve AGEs-induced impairment of intestinal barrier and disturbance of gut microbiota homeostasis, which in turn triggers neuroinflammation and oxidative stress via the microbiota-gut-brain (MGB) axis. Plant extracellular vesicles (PEVs), as natural nanocarriers, can not only enhance the bioavailability of polyphenols but also, in synergy with polyphenols, exhibit antioxidant, anti-inflammatory, and gut-microenvironment-modulating properties. This review summarizes and discusses the potential and mechanisms by which PEVs-polyphenols delivery systems may mitigate AGEs-related neurotoxicity by preserving MGB axis function, thereby providing a theoretical foundation for the development of prospective intervention strategies in this field.

饮食中过量摄入晚期糖基化终产物（AGEs）是神经退行性疾病（ndd）的潜在危险因素。其潜在机制可能涉及ages诱导的肠道屏障损伤和肠道微生物群稳态紊乱，进而通过微生物-肠-脑（MGB）轴引发神经炎症和氧化应激。植物细胞外囊泡（PEVs）作为天然的纳米载体，不仅可以提高多酚类物质的生物利用度，而且与多酚类物质协同作用，具有抗氧化、抗炎和调节肠道微环境的作用。本文综述并讨论了pevs -多酚递送系统通过保持MGB轴功能减轻衰老相关神经毒性的潜力和机制，从而为该领域前瞻性干预策略的发展提供理论基础。

引用次数: 0

Singlet Oxygen Generated by Quercetin and Gallic Acid Leads to Oxidative Fragmentation of Flavonols in Onions and Leek 槲皮素和没食子酸产生的单线态氧导致洋葱和韭菜黄酮醇氧化破碎

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.5c14934

Vanessa K. Fokuhl, Lea M. Kahl, Niels Heise, Marcus A. Glomb

For the first time, singlet oxygen was shown to be generated by quercetin and gallic acid under mild conditions (37 °C, pH 7) and exclusion of light. In contrast to kaempferol, quercetin induced its own oxidative fragmentation, yielding protocatechuic acid (3,4-dihydroxybenzoic acid) and 2,4,6-trihydroxybenzoic acid as the corresponding counterparts. If a 4-molar excess of gallic acid was coincubated, quercetin fragmentation reached 25 mol %, but now also kaempferol gave 7 mol % p-hydroxybenzoic acid. The hydroxybenzoic acids formed always showed the B-ring substitution pattern of the precursor flavonol. This reactive oxygen chemical quenching mechanism initiated by pericyclic cycloadditions was underlined by the use of singlet oxygen released from endoperoxides. Isolation and characterization of a quercetin–methanol solvent adduct pointed toward the parallel occurrence of physical quenching. The importance of singlet oxygen-mediated flavonol degradation in food matrices was verified by the detection of the fragmentation products in minced onion and leek samples.

首次证明槲皮素和没食子酸在温和条件（37℃，pH 7）和避光条件下产生单线态氧。与山奈酚相反，槲皮素诱导自身氧化断裂，生成相应的原儿茶酸（3,4-二羟基苯甲酸）和2,4,6-三羟基苯甲酸。如果4摩尔过量的没食子酸共孵化，槲皮素的破碎率达到25摩尔%，但山奈酚也会产生7摩尔%的对羟基苯甲酸。生成的羟基苯甲酸均表现为前体黄酮醇的b环取代模式。内过氧化物释放的单线态氧强调了由周环加成引发的活性氧化学猝灭机制。槲皮素-甲醇溶剂加合物的分离和表征表明了物理猝灭的平行发生。通过对洋葱和韭菜碎状产物的检测，验证了单重态氧介导的黄酮醇降解在食品基质中的重要性。

{"title":"Singlet Oxygen Generated by Quercetin and Gallic Acid Leads to Oxidative Fragmentation of Flavonols in Onions and Leek","authors":"Vanessa K. Fokuhl, Lea M. Kahl, Niels Heise, Marcus A. Glomb","doi":"10.1021/acs.jafc.5c14934","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c14934","url":null,"abstract":"For the first time, singlet oxygen was shown to be generated by quercetin and gallic acid under mild conditions (37 °C, pH 7) and exclusion of light. In contrast to kaempferol, quercetin induced its own oxidative fragmentation, yielding protocatechuic acid (3,4-dihydroxybenzoic acid) and 2,4,6-trihydroxybenzoic acid as the corresponding counterparts. If a 4-molar excess of gallic acid was coincubated, quercetin fragmentation reached 25 mol %, but now also kaempferol gave 7 mol % p-hydroxybenzoic acid. The hydroxybenzoic acids formed always showed the B-ring substitution pattern of the precursor flavonol. This reactive oxygen chemical quenching mechanism initiated by pericyclic cycloadditions was underlined by the use of singlet oxygen released from endoperoxides. Isolation and characterization of a quercetin–methanol solvent adduct pointed toward the parallel occurrence of physical quenching. The importance of singlet oxygen-mediated flavonol degradation in food matrices was verified by the detection of the fragmentation products in minced onion and leek samples.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146116228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenol-Rich Coffee Leaf Extract Alleviates High-Fat Diet-Induced Intestinal Barrier Dysfunction through Modulation of Barrier Integrity, Enterohepatic Axis, and Gut Microbiota. 富含多酚的咖啡叶提取物通过调节屏障完整性、肠肝轴和肠道微生物群，缓解高脂肪饮食诱导的肠道屏障功能障碍。

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.5c13455

Suhuan Mei, Qingwei Cao, Gongping Huang, Dongyan Chen, David D Kitts, Xiumin Chen

A polyphenol-rich extract (WEAC) from coffee leaf was previously shown to protect the epithelial barrier integrity. This study investigated the protective effects of WEAC in C57BL/6 mice fed a high-fat diet (HFD). WEAC supplementation (100-200 mg/kg·bw) reduced body weight and lowered TNF-α levels in serum, colon, liver, and brain in mice. WEAC improved mouse intestinal barrier integrity by upregulating the tight-junction protein and reducing intestinal d-lactic acid leakage. Liver histology revealed reduced lipid accumulation and ballooning degeneration, corresponding to decreased triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels. The WEAC intervention also decreased total bile acid levels and altered short-chain fatty acid profiles and key phytochemical metabolism. Furthermore, WEAC reduced the Firmicutes/Bacteroidetes ratio, increased the Parabacteroides, unclassified_Muribaculaceae, and Akkermansia abundance, and lowered the Blautia levels. Additionally, WEAC showed no adverse effects in mice fed a normal diet. Overall, WEAC mitigated HFD-induced intestinal barrier damage and enterohepatic function, reduced systemic inflammation, and stabilized the gut microbiome.

从咖啡叶中提取的富含多酚的提取物（WEAC）先前被证明可以保护上皮屏障的完整性。本研究探讨了WEAC对饲喂高脂饲料（HFD）的C57BL/6小鼠的保护作用。添加WEAC （100 ~ 200 mg/kg·bw）可降低小鼠体重，降低血清、结肠、肝脏和脑中TNF-α水平。WEAC通过上调紧密连接蛋白，减少肠道d-乳酸渗漏，改善小鼠肠道屏障完整性。肝脏组织学显示脂质积累和球囊变性减少，相应的甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平降低。WEAC干预还降低了总胆汁酸水平，改变了短链脂肪酸谱和关键的植物化学代谢。此外，WEAC降低了厚壁菌门(Firmicutes) /拟杆菌门（Bacteroidetes）的比例，增加了副杆菌门（Parabacteroides）、未分类菌科（unclassified_Muribaculaceae）和Akkermansia的丰度，降低了蓝藻门（Blautia）的水平。此外，WEAC对正常饮食的小鼠没有不良影响。总体而言，WEAC减轻了hfd诱导的肠屏障损伤和肠肝功能，减少了全身炎症，并稳定了肠道微生物群。

{"title":"Polyphenol-Rich Coffee Leaf Extract Alleviates High-Fat Diet-Induced Intestinal Barrier Dysfunction through Modulation of Barrier Integrity, Enterohepatic Axis, and Gut Microbiota.","authors":"Suhuan Mei, Qingwei Cao, Gongping Huang, Dongyan Chen, David D Kitts, Xiumin Chen","doi":"10.1021/acs.jafc.5c13455","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c13455","url":null,"abstract":"A polyphenol-rich extract (WEAC) from coffee leaf was previously shown to protect the epithelial barrier integrity. This study investigated the protective effects of WEAC in C57BL/6 mice fed a high-fat diet (HFD). WEAC supplementation (100-200 mg/kg·bw) reduced body weight and lowered TNF-α levels in serum, colon, liver, and brain in mice. WEAC improved mouse intestinal barrier integrity by upregulating the tight-junction protein and reducing intestinal d-lactic acid leakage. Liver histology revealed reduced lipid accumulation and ballooning degeneration, corresponding to decreased triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels. The WEAC intervention also decreased total bile acid levels and altered short-chain fatty acid profiles and key phytochemical metabolism. Furthermore, WEAC reduced the Firmicutes/Bacteroidetes ratio, increased the Parabacteroides, unclassified_Muribaculaceae, and Akkermansia abundance, and lowered the Blautia levels. Additionally, WEAC showed no adverse effects in mice fed a normal diet. Overall, WEAC mitigated HFD-induced intestinal barrier damage and enterohepatic function, reduced systemic inflammation, and stabilized the gut microbiome.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GsSnRK1-Dependent Degradation of the E3 Ubiquitin Ligase GsSRF2 Positively Regulates Soybean Resistance to Salt Stress E3泛素连接酶GsSRF2依赖性降解正调控大豆对盐胁迫的抗性

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.5c10047

Siyu Liu, Minglong Li, Xin Liu, Qi Sun, Peng Feng, Qiang Li, Jialei Xiao, Pengfei Xu, Shuzhen Zhang, Xiaodong Ding

Protein phosphorylation and ubiquitination play central roles in signal transduction. SnRK1 is a key kinase that mediates plant responses to environmental cues. From a wild soybean cDNA library, we identified GsSRF2, which encodes a RING-domain E3 ligase that interacts with GsSnRK1. GsSRF2 is predominantly expressed in roots and responds dynamically to salt stress. BiFC, pull-down, and co-IP assays this interaction in the cytoplasm and at the plasma membrane. Biochemical analyses revealed that GsSnRK1 phosphorylates GsSRF2 at Thr514. GnSnRK1-mediated phosphorylation enhanced GsSRF2 ubiquitination and promoted its proteasomal degradation. Coexpression assays in soybean hairy roots demonstrated that the GsSnRK1–GsSRF2 module significantly improved salt tolerance, whereas the GsSnRK1–GsSRF2(T514A) and kinase-dead GsSnRK1(K49M)–GsSRF2 modules reduced salt resistance in composite plants. Together, these results indicate that GsSnRK1 positively regulates soybean salt tolerance by modulating GsSRF2 ligase activity and protein stability, providing valuable genetic resources for crop improvement.

蛋白磷酸化和泛素化在信号转导中起着核心作用。SnRK1是介导植物对环境信号反应的关键激酶。从野生大豆cDNA文库中，我们鉴定出GsSRF2，它编码一个与GsSnRK1相互作用的环域E3连接酶。GsSRF2主要在根系中表达，对盐胁迫有动态响应。BiFC、pull-down和co-IP检测细胞质和质膜上的这种相互作用。生化分析显示gsssnrk1磷酸化GsSRF2的Thr514位点。gnsnrk1介导的磷酸化增强了GsSRF2的泛素化，促进了其蛋白酶体降解。大豆毛状根共表达实验表明，GsSnRK1 -GsSRF2模块显著提高了复合植株的耐盐性，而GsSnRK1 -GsSRF2 （T514A）和激酶死亡的GsSnRK1(K49M) -GsSRF2模块降低了复合植株的耐盐性。综上所述，GsSnRK1通过调节GsSRF2连接酶活性和蛋白稳定性正向调节大豆耐盐性，为作物改良提供了宝贵的遗传资源。

{"title":"GsSnRK1-Dependent Degradation of the E3 Ubiquitin Ligase GsSRF2 Positively Regulates Soybean Resistance to Salt Stress","authors":"Siyu Liu, Minglong Li, Xin Liu, Qi Sun, Peng Feng, Qiang Li, Jialei Xiao, Pengfei Xu, Shuzhen Zhang, Xiaodong Ding","doi":"10.1021/acs.jafc.5c10047","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c10047","url":null,"abstract":"Protein phosphorylation and ubiquitination play central roles in signal transduction. SnRK1 is a key kinase that mediates plant responses to environmental cues. From a wild soybean cDNA library, we identified GsSRF2, which encodes a RING-domain E3 ligase that interacts with GsSnRK1. GsSRF2 is predominantly expressed in roots and responds dynamically to salt stress. BiFC, pull-down, and co-IP assays this interaction in the cytoplasm and at the plasma membrane. Biochemical analyses revealed that GsSnRK1 phosphorylates GsSRF2 at Thr514. GnSnRK1-mediated phosphorylation enhanced GsSRF2 ubiquitination and promoted its proteasomal degradation. Coexpression assays in soybean hairy roots demonstrated that the GsSnRK1–GsSRF2 module significantly improved salt tolerance, whereas the GsSnRK1–GsSRF2(T514A) and kinase-dead GsSnRK1(K49M)–GsSRF2 modules reduced salt resistance in composite plants. Together, these results indicate that GsSnRK1 positively regulates soybean salt tolerance by modulating GsSRF2 ligase activity and protein stability, providing valuable genetic resources for crop improvement.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"24 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Field Overexpression to Biochemical Function: PxCYP6B4-Mediated Hydroxylation Confers Pyrethroid Resistance in Plutella xylostella. 从田间过表达到生化功能：pxcyp6b4介导的羟基化作用赋予小菜蛾拟除虫菊酯抗性

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.6c00843

Zhuoda Liu, Yifei Zhou, Dongliang Wang, Ruichi Li, Zhen Tian, Yifan Li, Jiyuan Liu

The rapid evolution of insecticide resistance threatens global food security, with cytochrome P450-mediated detoxification representing a key adaptive mechanism in insects. However, direct biochemical evidence linking specific P450s to pyrethroid metabolism in Plutella xylostella remains limited. Here, we investigated the molecular basis of high-level pyrethroid resistance in P. xylostella. Comparative transcriptomic analyses of a susceptible strain and two highly resistant field populations (resistance ratios >650-fold) identified PxCYP6B4 as the most strongly overexpressed P450 (>95-fold). RNA interference-mediated knockdown of PxCYP6B4 significantly increased larval susceptibility to pyrethroids, while heterologous expression in Drosophila melanogaster conferred a 5.27-fold increase in tolerance to lambda-cyhalothrin. Importantly, in vitro assays using recombinant PxCYP6B4 expressed in Sf9 cells, combined with LC-MS/MS analysis, demonstrated direct hydroxylation of lambda-cyhalothrin to 4'-hydroxy-lambda-cyhalothrin. These results establish a direct molecular and biochemical link between PxCYP6B4 overexpression and pyrethroid detoxification.

杀虫剂抗性的快速进化威胁着全球粮食安全，细胞色素p450介导的解毒是昆虫的关键适应机制。然而，将特定p450与小菜蛾的拟除虫菊酯代谢联系起来的直接生化证据仍然有限。本文研究了小菜蛾高水平拟除虫菊酯类抗性的分子基础。比较易感菌株和两个高抗性田间群体（抗性比>650倍）的转录组学分析发现，PxCYP6B4是最强烈过表达的P450（>95倍）。RNA干扰介导的PxCYP6B4敲低显著增加了幼虫对拟除虫菊酯的敏感性，而在黑腹果蝇中异源表达使其对高效氯氟氰菊酯的耐受性增加了5.27倍。重要的是，利用在Sf9细胞中表达的重组cyp6b4进行体外实验，结合LC-MS/MS分析，证实了高效氯氟氰菊酯直接羟基化为4'-羟基-高效氯氟氰菊酯。这些结果在PxCYP6B4过表达和拟除虫菊酯解毒之间建立了直接的分子和生化联系。

{"title":"From Field Overexpression to Biochemical Function: PxCYP6B4-Mediated Hydroxylation Confers Pyrethroid Resistance in Plutella xylostella.","authors":"Zhuoda Liu, Yifei Zhou, Dongliang Wang, Ruichi Li, Zhen Tian, Yifan Li, Jiyuan Liu","doi":"10.1021/acs.jafc.6c00843","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c00843","url":null,"abstract":"The rapid evolution of insecticide resistance threatens global food security, with cytochrome P450-mediated detoxification representing a key adaptive mechanism in insects. However, direct biochemical evidence linking specific P450s to pyrethroid metabolism in Plutella xylostella remains limited. Here, we investigated the molecular basis of high-level pyrethroid resistance in P. xylostella. Comparative transcriptomic analyses of a susceptible strain and two highly resistant field populations (resistance ratios >650-fold) identified PxCYP6B4 as the most strongly overexpressed P450 (>95-fold). RNA interference-mediated knockdown of PxCYP6B4 significantly increased larval susceptibility to pyrethroids, while heterologous expression in Drosophila melanogaster conferred a 5.27-fold increase in tolerance to lambda-cyhalothrin. Importantly, in vitro assays using recombinant PxCYP6B4 expressed in Sf9 cells, combined with LC-MS/MS analysis, demonstrated direct hydroxylation of lambda-cyhalothrin to 4'-hydroxy-lambda-cyhalothrin. These results establish a direct molecular and biochemical link between PxCYP6B4 overexpression and pyrethroid detoxification.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polarity-Switching Photoelectrochemical Detection of Ochratoxin A Based on Target-Responsive Dendritic Mesoporous Silica Nanoparticle and Z-Scheme Hemin/HOF-101 Heterostructure 基于目标响应树突状介孔二氧化硅纳米颗粒和Z-Scheme Hemin/HOF-101异质结构的极性开关光电化学检测赭曲霉毒素A

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-02-05 DOI: 10.1021/acs.jafc.5c14258

Ke Xiao, Sitong Huang, Jia Sun, Zhen Liu, Leixia Meng

A novel polarity-switching photoelectrochemical (PEC) sensing method was proposed for quantifying ochratoxin A (OTA). Aminated dendritic mesoporous silica nanoparticle (DMSN) with well-defined pores was synthesized to encapsulate hemin, and its pore channels were sealed by OTA-specific aptamers as gatekeepers. Specific binding of OTA to the aptamer induced conformational changes and aptamer dissociation, triggering the release of entrapped hemin. The released hemin was captured by a hydrogen-bonded organic framework (HOF-101) modified electrode, which switched the photocurrent polarity from cathodic to anodic, with the anodic signal linearly increasing with OTA concentration. Under optimized conditions, the method exhibited a linear range of 10 pg mL^–1–10 μg mL^–1 and a limit of detection (LOD) of 3.5 pg mL^–1. Validated with camellia oil samples, its results were comparable to high-performance liquid chromatography (HPLC), verifying reliability for OTA detection in complex food matrices.

提出了一种新的极性开关光电化学（PEC）检测赭曲霉毒素A （OTA）的方法。合成了具有良好孔隙的胺化树突状介孔二氧化硅纳米颗粒（dmmsn）来包裹血红蛋白，其孔隙通道由ota特异性适配体作为守门人封闭。OTA与适体的特异性结合诱导构象变化和适体解离，触发被困血红蛋白的释放。释放的血红蛋白被氢键有机骨架（HOF-101）修饰电极捕获，该电极将光电流极性从阴极转换为阳极，阳极信号随OTA浓度线性增加。在优化条件下，该方法线性范围为10 pg mL-1 ~ 10 μg mL-1，检出限为3.5 pg mL-1。经油茶油样品验证，其结果与高效液相色谱（HPLC）相当，验证了OTA检测复杂食品基质的可靠性。

{"title":"Polarity-Switching Photoelectrochemical Detection of Ochratoxin A Based on Target-Responsive Dendritic Mesoporous Silica Nanoparticle and Z-Scheme Hemin/HOF-101 Heterostructure","authors":"Ke Xiao, Sitong Huang, Jia Sun, Zhen Liu, Leixia Meng","doi":"10.1021/acs.jafc.5c14258","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c14258","url":null,"abstract":"A novel polarity-switching photoelectrochemical (PEC) sensing method was proposed for quantifying ochratoxin A (OTA). Aminated dendritic mesoporous silica nanoparticle (DMSN) with well-defined pores was synthesized to encapsulate hemin, and its pore channels were sealed by OTA-specific aptamers as gatekeepers. Specific binding of OTA to the aptamer induced conformational changes and aptamer dissociation, triggering the release of entrapped hemin. The released hemin was captured by a hydrogen-bonded organic framework (HOF-101) modified electrode, which switched the photocurrent polarity from cathodic to anodic, with the anodic signal linearly increasing with OTA concentration. Under optimized conditions, the method exhibited a linear range of 10 pg mL–1–10 μg mL–1 and a limit of detection (LOD) of 3.5 pg mL–1. Validated with camellia oil samples, its results were comparable to high-performance liquid chromatography (HPLC), verifying reliability for OTA detection in complex food matrices.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"302 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

首页上一页

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Journal of Agricultural and Food Chemistry

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀