Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.5c14956
Yuntong Liang,Zhongquan Peng,Qiaoyuan Luo,Baofeng Lin
The prevention and control of rice pests and diseases are critical to crop quality. Traditional approaches rely heavily on high-concentration pesticides and antibacterial agents, imposing severe environmental burdens. Based on the coordination between tannic acid (TA) and unsaturated Zn2+ on zeolitic imidazolate framework-8 (ZIF-8), we innovatively fabricated a antibacterial seed coating TA/ZIF-8@SA (TZS) loaded with thiamethoxam (TMX). The dense Zn2+-TA gating network and ZIF-8 double-layer structure enable precise and controllable TMX release, reducing pesticide-induced environmental pollution. This coating extends TMX release by over 15 times relative to commercial formulations. In pot experiments, TMX-TZS exerted persistent control against rice planthoppers with 73.54% control efficacy at 30 days, far exceeding 31% for pure TMX. It also achieved a germination rate of 75.43 ± 6.67% in Fusarium moniliforme-infected soil, demonstrating strong antifungal activity. Therefore, TMX-TZS integrates insecticidal and antibacterial functions to strengthen crop defense, realizing the dual control of rice diseases and pests.
{"title":"Ultrasustained Dual Control of Rice Diseases and Pests via Zn2+-Tannic Acid-Gated Zeolite Imidazole Framework@Sodium Alginate Multifunctional Seed Coating.","authors":"Yuntong Liang,Zhongquan Peng,Qiaoyuan Luo,Baofeng Lin","doi":"10.1021/acs.jafc.5c14956","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c14956","url":null,"abstract":"The prevention and control of rice pests and diseases are critical to crop quality. Traditional approaches rely heavily on high-concentration pesticides and antibacterial agents, imposing severe environmental burdens. Based on the coordination between tannic acid (TA) and unsaturated Zn2+ on zeolitic imidazolate framework-8 (ZIF-8), we innovatively fabricated a antibacterial seed coating TA/ZIF-8@SA (TZS) loaded with thiamethoxam (TMX). The dense Zn2+-TA gating network and ZIF-8 double-layer structure enable precise and controllable TMX release, reducing pesticide-induced environmental pollution. This coating extends TMX release by over 15 times relative to commercial formulations. In pot experiments, TMX-TZS exerted persistent control against rice planthoppers with 73.54% control efficacy at 30 days, far exceeding 31% for pure TMX. It also achieved a germination rate of 75.43 ± 6.67% in Fusarium moniliforme-infected soil, demonstrating strong antifungal activity. Therefore, TMX-TZS integrates insecticidal and antibacterial functions to strengthen crop defense, realizing the dual control of rice diseases and pests.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"36 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.6c02407
Akshara Pratheep,Rashada Rauf,Aleesha Augustine,Ravi Baraiya,Radhika Rajasree S R
{"title":"Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (RuBisCO)-Derived Peptides from Seaweeds: An Emerging Redox-Driven Paradigm for Immunomodulation.","authors":"Akshara Pratheep,Rashada Rauf,Aleesha Augustine,Ravi Baraiya,Radhika Rajasree S R","doi":"10.1021/acs.jafc.6c02407","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c02407","url":null,"abstract":"","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"91 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aging is accompanied by chronic low-grade inflammation (inflammaging), driving age-related diseases. Urolithin A (UA), a gut microbial metabolite, possesses anti-inflammatory properties, yet its mechanism in hepatic aging remains unclear. This study investigated UA’s effects on aging-associated inflammation and the involvement of Nur77 in D-galactose-induced macrophage senescence and mouse liver aging models using molecular docking, Western blotting, and immunoprecipitation. UA alleviated cellular senescence markers (p53, p21), suppressed pro-inflammatory factors (IL-6, IL-1β), and elevated anti-inflammatory IL-10. Mechanistically, UA enhanced Nur77 protein stability by inhibiting MDM2-mediated ubiquitination and degradation, thereby restoring inflammatory homeostasis. In vivo, UA ameliorated D-gal-induced liver injury and modulated the hepatic Nur77-MDM2 axis. Conclusion: UA stabilizes Nur77 by inhibiting its ubiquitination, alleviating hepatic aging-associated inflammation. This study identifies the MDM2-Nur77 axis as a potential therapeutic target for hepatic aging.
{"title":"Urolithin A Attenuates Aging-Induced Liver Injury by Inhibiting Nur77 Ubiquitination and Degradation","authors":"Jiaqi Xiao, Lihua Qu, Xuan Qin, Chao Chen, Yuan Xu, Xinyu Que, Yaoyao Ma, Wentao Huang, Haoxiang Ou, Chao Wu, Yongfen Bao, Shigang Shan","doi":"10.1021/acs.jafc.5c17898","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c17898","url":null,"abstract":"Aging is accompanied by chronic low-grade inflammation (inflammaging), driving age-related diseases. Urolithin A (UA), a gut microbial metabolite, possesses anti-inflammatory properties, yet its mechanism in hepatic aging remains unclear. This study investigated UA’s effects on aging-associated inflammation and the involvement of Nur77 in D-galactose-induced macrophage senescence and mouse liver aging models using molecular docking, Western blotting, and immunoprecipitation. UA alleviated cellular senescence markers (p53, p21), suppressed pro-inflammatory factors (IL-6, IL-1β), and elevated anti-inflammatory IL-10. Mechanistically, UA enhanced Nur77 protein stability by inhibiting MDM2-mediated ubiquitination and degradation, thereby restoring inflammatory homeostasis. In vivo, UA ameliorated D-gal-induced liver injury and modulated the hepatic Nur77-MDM2 axis. Conclusion: UA stabilizes Nur77 by inhibiting its ubiquitination, alleviating hepatic aging-associated inflammation. This study identifies the MDM2-Nur77 axis as a potential therapeutic target for hepatic aging.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147462200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.5c17281
Hui Ding, Jie Zhang, Joe M. Regenstein, Yipin Lyu, Thom Huppertz, Peng Zhou
Lactopontin (LPN) plays a critical role in the growth and development of infants. Caprine milk and bovine milk are the primary raw materials for infant formulas. However, the differences between caprine and bovine LPN have not been studied. In this study, the structure, digestive characteristics, and active peptide composition of LPN from different sources were compared. Two N-glycosylation sites, Asn101 and Asn208, were identified in bovine LPN, while a single N-glycosylation site, Asn79, was found in caprine LPN. The in vitro infant digestion simulation results indicated that caprine LPN released a greater quantity of small peptides and amino acids. The intestinal digestion products were subsequently analyzed. The digestive peptides derived from caprine LPN may possess various potential biological functions. These findings provide insights into optimizing protein digestion and nutrient absorption in infant formula.
{"title":"The Structure and Digestive Characteristics of Caprine and Bovine Lactopontin","authors":"Hui Ding, Jie Zhang, Joe M. Regenstein, Yipin Lyu, Thom Huppertz, Peng Zhou","doi":"10.1021/acs.jafc.5c17281","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c17281","url":null,"abstract":"Lactopontin (LPN) plays a critical role in the growth and development of infants. Caprine milk and bovine milk are the primary raw materials for infant formulas. However, the differences between caprine and bovine LPN have not been studied. In this study, the structure, digestive characteristics, and active peptide composition of LPN from different sources were compared. Two N-glycosylation sites, Asn101 and Asn208, were identified in bovine LPN, while a single N-glycosylation site, Asn79, was found in caprine LPN. The <i>in vitro</i> infant digestion simulation results indicated that caprine LPN released a greater quantity of small peptides and amino acids. The intestinal digestion products were subsequently analyzed. The digestive peptides derived from caprine LPN may possess various potential biological functions. These findings provide insights into optimizing protein digestion and nutrient absorption in infant formula.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"52 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147462198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.5c15354
Yiyan Liu,Bing Li,Tianning Zhang,Yang Jiang,Dapeng Li
Obesity is increasingly linked to high-fat, fiber-deficient dietary patterns that disturb the intestinal microenvironment and gut microbiota. Here, we tested whether pectin molecular weight determines efficacy by comparing high-molecular-weight apple pectin (HAP) with low-molecular-weight pectin (LAP) in a fiber-free, high-fat-diet (FF-HFD) mouse model. In young C57BL/6J mice, HAP more strongly improved body weight gain, glucose homeostasis, and lipid abnormalities than LAP, accompanied by enhanced barrier markers, reduced systemic inflammation, lower brain LPS, and partial normalization of fecal microbiota and fermentation outputs. Segment-resolved profiling further showed that HAP partially restored proximal-distal luminal microbiota patterns disrupted by FF-HFD. In an aged cohort, HAP also alleviated obesity-associated hepatic steatosis and behavioral deficits. These findings highlight molecular weight as a practical lever to optimize pectin-based interventions under fiber-deficient, high-fat feeding.
{"title":"High-Molecular-Weight Pectin Alleviates Fiber-Free High-Fat Diet-Induced Obesity by Regulating the Colonic Microenvironment and Spatial Microbiota Distribution.","authors":"Yiyan Liu,Bing Li,Tianning Zhang,Yang Jiang,Dapeng Li","doi":"10.1021/acs.jafc.5c15354","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c15354","url":null,"abstract":"Obesity is increasingly linked to high-fat, fiber-deficient dietary patterns that disturb the intestinal microenvironment and gut microbiota. Here, we tested whether pectin molecular weight determines efficacy by comparing high-molecular-weight apple pectin (HAP) with low-molecular-weight pectin (LAP) in a fiber-free, high-fat-diet (FF-HFD) mouse model. In young C57BL/6J mice, HAP more strongly improved body weight gain, glucose homeostasis, and lipid abnormalities than LAP, accompanied by enhanced barrier markers, reduced systemic inflammation, lower brain LPS, and partial normalization of fecal microbiota and fermentation outputs. Segment-resolved profiling further showed that HAP partially restored proximal-distal luminal microbiota patterns disrupted by FF-HFD. In an aged cohort, HAP also alleviated obesity-associated hepatic steatosis and behavioral deficits. These findings highlight molecular weight as a practical lever to optimize pectin-based interventions under fiber-deficient, high-fat feeding.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"34 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.5c08843
Chanchan Xu,Shaoyu Tang,Hua Yin,Zhi Dang
Nanoplastics (NPs) and salinity increasingly co-occur in agricultural systems. Here, we investigated how polystyrene NPs (502 nm, 10 mg L-1) impair rice (Oryza sativa L.) recovery from salt (50 mM NaCl). During stress, NPs synergistically amplified ionic toxicity, elevating Na+/K+ ratios 48% above additive predictions. Crucially, this synergism intensified to 60% during recovery, preventing homeostasis restoration. Transcriptomic analysis revealed that NP-salt interactions shifted from additive to antagonistic poststress, disrupting trehalose pathway regulation critical for osmotic adjustment. Additionally, coexposed plants failed to switch from stress- to growth-associated gene modules, exhibiting 34% fewer differentially expressed genes than salt-only plants. These findings demonstrate that NPs compromise transcriptional resilience by disrupting adaptive reprogramming, emphasizing the need for recovery-inclusive risk assessments.
{"title":"Polystyrene Nanoplastics Impair Transcriptional Resilience to Salt Stress in Rice.","authors":"Chanchan Xu,Shaoyu Tang,Hua Yin,Zhi Dang","doi":"10.1021/acs.jafc.5c08843","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08843","url":null,"abstract":"Nanoplastics (NPs) and salinity increasingly co-occur in agricultural systems. Here, we investigated how polystyrene NPs (502 nm, 10 mg L-1) impair rice (Oryza sativa L.) recovery from salt (50 mM NaCl). During stress, NPs synergistically amplified ionic toxicity, elevating Na+/K+ ratios 48% above additive predictions. Crucially, this synergism intensified to 60% during recovery, preventing homeostasis restoration. Transcriptomic analysis revealed that NP-salt interactions shifted from additive to antagonistic poststress, disrupting trehalose pathway regulation critical for osmotic adjustment. Additionally, coexposed plants failed to switch from stress- to growth-associated gene modules, exhibiting 34% fewer differentially expressed genes than salt-only plants. These findings demonstrate that NPs compromise transcriptional resilience by disrupting adaptive reprogramming, emphasizing the need for recovery-inclusive risk assessments.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"3 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.5c15321
Nuo Li, Xiaobao Sun, Jiahui Lu, Chong-En Chu, Xiaofeng Xu, Junyan Han, Mingqi Liu, Yu Liu, Jiakun Wang, Qian Wang
Lytic polysaccharide monooxygenase (LPMO) assists the degradation of recalcitrant polysaccharides in the cell wall. In this study, eight LPMOs with C1-, C4-, or C1/C4-acting modes were employed to understand their interactions with various glycoside hydrolases (GHs). Dynamic viscosity and reducing sugar measurements indicated that C1-LPMO specifically enhanced cellobiohydrolase (CBH) II activity while suppressing CBH I, whereas C4-LPMO selectively promoted CBH I but inhibited CBH II, demonstrating LPMO’s regioselective modulation of GHs. Fluorescence microscopy and inhibition kinetics revealed that the inhibitory effects were mainly attributable to substrate competition at the early stage of coreaction and feedback inhibition by oxidized oligosaccharides produced by LPMO. Notably, coimmobilization of BsLPMO10A from Bacillus subtilis with Cellic CTec3 was effective against natural biomass substrates (p < 0.05), compared with the mixture of free enzymes. These findings provided novel insights into understanding and mitigating the inhibitory effects of LPMO on GH, enabling efficient saccharification of lignocellulosic plant biomass.
{"title":"Understanding and Mitigating the Inhibitory Effects of Lytic Polysaccharide Monooxygenase on Glycoside Hydrolases at the Early Stage of Coreaction","authors":"Nuo Li, Xiaobao Sun, Jiahui Lu, Chong-En Chu, Xiaofeng Xu, Junyan Han, Mingqi Liu, Yu Liu, Jiakun Wang, Qian Wang","doi":"10.1021/acs.jafc.5c15321","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c15321","url":null,"abstract":"Lytic polysaccharide monooxygenase (LPMO) assists the degradation of recalcitrant polysaccharides in the cell wall. In this study, eight LPMOs with C1-, C4-, or C1/C4-acting modes were employed to understand their interactions with various glycoside hydrolases (GHs). Dynamic viscosity and reducing sugar measurements indicated that C1-LPMO specifically enhanced cellobiohydrolase (CBH) II activity while suppressing CBH I, whereas C4-LPMO selectively promoted CBH I but inhibited CBH II, demonstrating LPMO’s regioselective modulation of GHs. Fluorescence microscopy and inhibition kinetics revealed that the inhibitory effects were mainly attributable to substrate competition at the early stage of coreaction and feedback inhibition by oxidized oligosaccharides produced by LPMO. Notably, coimmobilization of BsLPMO10A from <i>Bacillus subtilis</i> with Cellic CTec3 was effective against natural biomass substrates (<i>p</i> < 0.05), compared with the mixture of free enzymes. These findings provided novel insights into understanding and mitigating the inhibitory effects of LPMO on GH, enabling efficient saccharification of lignocellulosic plant biomass.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147462197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.6c00881
Wenting Ji, Guijie Chen, Jianhua Zeng, Yanhui Feng, Guoyuan Xiong, Chuanlai Du, Xiaoxiong Zeng, Chunxu Chen
This study synthesized and structurally validated theasinensin C (TSC, > 98%) and evaluated its neuroprotective effects in a high-fructose diet (HFrD)-induced mouse model of neuroinflammation. TSC (150 mg/kg/day, 8 weeks) improved cognition, reduced pro-inflammatory cytokines, mitigated neuropathology, and restored intestinal barrier integrity. Concomitantly, TSC remodeled the gut microbiota, selectively enriching Akkermansia muciniphila. We isolated A. muciniphila XJ 240720 and demonstrated that its synergy with TSC elevated creatine in serum and the brain, identifying creatine as a key mediator. Multiomics analyses showed that TSC enhanced A. muciniphila hydrolysis of mucin proline-threonine-serine (PTS) domains, releasing proline (Pro) and serine (Ser), and promoted the conversion of Pro via glutamate (Glu) to glutamine (Gln), driving luminal Gln and Ser accumulation. Enterocytes subsequently converted Gln to citrulline (Cit) and aspartate (Asp). In the kidney, the Cit/Asp→arginine (Arg) route coupled with the Ser→glycine (Gly) pathway generated guanidinoacetate (GAA), which the liver methylated via S-adenosylmethionine (SAM) to creatine. Circulating creatine reached the brain and suppressed neuroinflammation. Gln and Ser Supplementation in germ-free mice reproduced behavioral and antineuroinflammatory effects. Thus, TSC enriches A. muciniphila and drives a Gln/Ser-centered multiorgan creatine pathway that alleviates diet-induced neuroinflammation and informs gut-brain axis interventions.
{"title":"Theasinensin C Mitigates HFrD-Induced Neuroinflammation by Enriching Akkermansia muciniphila and Orchestrating a Gln/Ser-Centered Multiorgan Metabolic Relay to Drive Creatine Biosynthesis","authors":"Wenting Ji, Guijie Chen, Jianhua Zeng, Yanhui Feng, Guoyuan Xiong, Chuanlai Du, Xiaoxiong Zeng, Chunxu Chen","doi":"10.1021/acs.jafc.6c00881","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c00881","url":null,"abstract":"This study synthesized and structurally validated theasinensin C (TSC, > 98%) and evaluated its neuroprotective effects in a high-fructose diet (HFrD)-induced mouse model of neuroinflammation. TSC (150 mg/kg/day, 8 weeks) improved cognition, reduced pro-inflammatory cytokines, mitigated neuropathology, and restored intestinal barrier integrity. Concomitantly, TSC remodeled the gut microbiota, selectively enriching <i>Akkermansia muciniphila</i>. We isolated <i>A. muciniphila</i> XJ 240720 and demonstrated that its synergy with TSC elevated creatine in serum and the brain, identifying creatine as a key mediator. Multiomics analyses showed that TSC enhanced <i>A. muciniphila</i> hydrolysis of mucin proline-threonine-serine (PTS) domains, releasing proline (Pro) and serine (Ser), and promoted the conversion of Pro via glutamate (Glu) to glutamine (Gln), driving luminal Gln and Ser accumulation. Enterocytes subsequently converted Gln to citrulline (Cit) and aspartate (Asp). In the kidney, the Cit/Asp→arginine (Arg) route coupled with the Ser→glycine (Gly) pathway generated guanidinoacetate (GAA), which the liver methylated via <i>S</i>-adenosylmethionine (SAM) to creatine. Circulating creatine reached the brain and suppressed neuroinflammation. Gln and Ser Supplementation in germ-free mice reproduced behavioral and antineuroinflammatory effects. Thus, TSC enriches <i>A. muciniphila</i> and drives a Gln/Ser-centered multiorgan creatine pathway that alleviates diet-induced neuroinflammation and informs gut-brain axis interventions.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147462205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1021/acs.jafc.5c15071
Jiafeng Niu,Xiaoyu Zhu,Huibing Chi,Bin Ma,Hao Zhu,Zhaoxin Lu,Ping Zhu,Fengxia Lu
The bioremediation strategy is a promising environmentally friendly alternative to eliminate deoxynivalenol (DON) contamination in feed and foodstuffs, but few direct and effective enzyme-catalyzed detoxification methods are currently available. Here, we identified an aldo-keto reductase with DON-degrading ability from the Devosia strain A6-243 (AKR6D2) through a stepwise gene mining approach. AKR6D2 was found to have great thermostability toward the reduction of C3 carbonyl oxygen of 3-keto- deoxynivalenol (3-keto-DON) but with poor stereoselectivity (44.97% e.e. for 3-epi- deoxynivalenol (3-epi-DON)), which limited its practical application. By reshaping the substrate-binding pocket of AKR6D2, a mutant W21A/G53N with excellent catalytic activity (330-fold higher) and stereoselectivity (>99% e.e. for 3-epi-DON) was obtained. Based on the insights provided by molecular dynamics simulations, W21A/G53N-3-keto-DONproR was more favorable in the formation of prereaction states to produce the R-configuration product. This work confirmed that engineered AKR6D2 is a powerful biocatalyst for the detoxification of DON in contaminated food and feed.
{"title":"Reshaping the Binding Pocket of Aldo-Keto Reductase for Enhanced Stereoselectivity and Activity.","authors":"Jiafeng Niu,Xiaoyu Zhu,Huibing Chi,Bin Ma,Hao Zhu,Zhaoxin Lu,Ping Zhu,Fengxia Lu","doi":"10.1021/acs.jafc.5c15071","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c15071","url":null,"abstract":"The bioremediation strategy is a promising environmentally friendly alternative to eliminate deoxynivalenol (DON) contamination in feed and foodstuffs, but few direct and effective enzyme-catalyzed detoxification methods are currently available. Here, we identified an aldo-keto reductase with DON-degrading ability from the Devosia strain A6-243 (AKR6D2) through a stepwise gene mining approach. AKR6D2 was found to have great thermostability toward the reduction of C3 carbonyl oxygen of 3-keto- deoxynivalenol (3-keto-DON) but with poor stereoselectivity (44.97% e.e. for 3-epi- deoxynivalenol (3-epi-DON)), which limited its practical application. By reshaping the substrate-binding pocket of AKR6D2, a mutant W21A/G53N with excellent catalytic activity (330-fold higher) and stereoselectivity (>99% e.e. for 3-epi-DON) was obtained. Based on the insights provided by molecular dynamics simulations, W21A/G53N-3-keto-DONproR was more favorable in the formation of prereaction states to produce the R-configuration product. This work confirmed that engineered AKR6D2 is a powerful biocatalyst for the detoxification of DON in contaminated food and feed.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"21 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edible insects are gaining popularity worldwide, particularly in their processed forms. However, edible insect consumption remains limited for some consumers due to reports of allergic reactions. Additionally, insect allergies have been documented in patients with a history of dust mite and crustacean allergies, suggesting potential cross-reactivity. This study aimed to evaluate the cross-reactivity of proteins from edible crickets in Thai patients aged 7-17 years with diagnosed dust mite allergies who had never previously consumed insects. Our findings indicate that thermal processing alters protein solubility, as evidenced by more pronounced IgG signals in the insoluble fractions than in the soluble fractions. Furthermore, immunoblot and liquid chromatography-mass spectrometry analyses identified tropomyosin and actin as potential cross-reactive allergens between edible crickets and house dust mites. These results suggest that individuals with dust mite allergies may be at an increased risk of allergic reactions after consuming edible crickets.
{"title":"Serological Cross-Reactivity and Potential Allergenic Risk of Edible Cricket Proteins in Children and Adolescents with House Dust Mite Allergy.","authors":"Thanakrit Khammeethong,Pharima Phiriyangkul,Siriluk Khumsui,Pathitta Meeboongirt,Napa-Ai Suriyabhivadh,Chotika Yokthongwattana,Chama Inson,Yiwa Suksawat,Chomdao Sinthuvanich","doi":"10.1021/acs.jafc.5c12201","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c12201","url":null,"abstract":"Edible insects are gaining popularity worldwide, particularly in their processed forms. However, edible insect consumption remains limited for some consumers due to reports of allergic reactions. Additionally, insect allergies have been documented in patients with a history of dust mite and crustacean allergies, suggesting potential cross-reactivity. This study aimed to evaluate the cross-reactivity of proteins from edible crickets in Thai patients aged 7-17 years with diagnosed dust mite allergies who had never previously consumed insects. Our findings indicate that thermal processing alters protein solubility, as evidenced by more pronounced IgG signals in the insoluble fractions than in the soluble fractions. Furthermore, immunoblot and liquid chromatography-mass spectrometry analyses identified tropomyosin and actin as potential cross-reactive allergens between edible crickets and house dust mites. These results suggest that individuals with dust mite allergies may be at an increased risk of allergic reactions after consuming edible crickets.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"87 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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