Nanohydroxyapatite (nHA) is a highly promising candidate for sustainable agriculture due to its excellent biocompatibility and environmental friendliness. However, their practical application has long been hindered by poor dispersibility. Herein, we develop an in situ approach that couples phosphorylated carbon sphere (PCS) formation from waste orange peels with concurrent nHA nucleation, creating nHA/PCS composites as a root exudate-triggered nanofertilizer. Density functional theory (DFT) calculations show weak binding energy at the nHA/PCS–nHA interface, thereby inhibiting nHA nanorods agglomeration. Compared to commercial nanohydroxyapatite (nCHA), nHA/PCS demonstrates superior interface stability and sustained-release properties. The nHA/PCS treatment significantly enhances lettuce seedling growth by improving root architecture and stimulating oxalic acid secretion, increasing leaf fresh weight by 60.95% compared to that with nCHA treatments. As a scaffold, the PCS matrix chemically anchors nHA nanorods for a controlled release in response to root exudates. This work presents an innovative strategy for engineering stable nanofertilizer delivery systems.
{"title":"In-Situ Anchoring of Hydroxyapatite Nanorods on Waste Orange Peel-Derived Phosphorylated Carbon Spheres for Enhanced Fertilizer Stability","authors":"Xue Li,Jingxu Yang,Samira Karroum,Amine Bousseta,Yi Wen,Zhengjuan Yan,Yanjun Zhong,Dehua Xu,Tao Luo,Benhe Zhong,Youssef Habibi,Xinlong Wang","doi":"10.1021/acs.jafc.5c12812","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c12812","url":null,"abstract":"Nanohydroxyapatite (nHA) is a highly promising candidate for sustainable agriculture due to its excellent biocompatibility and environmental friendliness. However, their practical application has long been hindered by poor dispersibility. Herein, we develop an in situ approach that couples phosphorylated carbon sphere (PCS) formation from waste orange peels with concurrent nHA nucleation, creating nHA/PCS composites as a root exudate-triggered nanofertilizer. Density functional theory (DFT) calculations show weak binding energy at the nHA/PCS–nHA interface, thereby inhibiting nHA nanorods agglomeration. Compared to commercial nanohydroxyapatite (nCHA), nHA/PCS demonstrates superior interface stability and sustained-release properties. The nHA/PCS treatment significantly enhances lettuce seedling growth by improving root architecture and stimulating oxalic acid secretion, increasing leaf fresh weight by 60.95% compared to that with nCHA treatments. As a scaffold, the PCS matrix chemically anchors nHA nanorods for a controlled release in response to root exudates. This work presents an innovative strategy for engineering stable nanofertilizer delivery systems.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"295 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paracellular transport is the primary pathway for the intact absorption of the egg white-derived peptide QIGLF. This study aimed to explore the paracellular absorption process of QIGLF in Caco-2 cells using 4D-FastDIA proteomics. A total of 147 differentially expressed proteins (DEPs) were identified. Gene Ontology enrichment analysis revealed that key DEPs, including GRHL2, ECT2, and MARVELD2 involved in apical junction assembly and tight junctions, as well as ARHGEF18 and EphA2 in the plasma membrane, participate in regulating the paracellular absorption of QIGLF. KEGG pathway analysis further indicated that Toll-like receptors and nuclear factor-kappa B-signaling pathways, along with the phosphatidylinositol signaling system, were closely associated with this absorption process. Altogether, the findings of this study demonstrate that QIGLF may alter the expression or subcellular localization of tight junction proteins through these signaling pathways, thereby modulating intestinal tight junction permeability and facilitating its paracellular absorption.
{"title":"Underlying Paracellular Absorption Mechanism of Egg White-Derived Peptide QIGLF: Insight from 4D-FastDIA Proteomic","authors":"Zhipeng Yu,Di Liu,Li Fu,Lingyun Gu,Long Ding,Wenzhu Zhao","doi":"10.1021/acs.jafc.5c17078","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c17078","url":null,"abstract":"Paracellular transport is the primary pathway for the intact absorption of the egg white-derived peptide QIGLF. This study aimed to explore the paracellular absorption process of QIGLF in Caco-2 cells using 4D-FastDIA proteomics. A total of 147 differentially expressed proteins (DEPs) were identified. Gene Ontology enrichment analysis revealed that key DEPs, including GRHL2, ECT2, and MARVELD2 involved in apical junction assembly and tight junctions, as well as ARHGEF18 and EphA2 in the plasma membrane, participate in regulating the paracellular absorption of QIGLF. KEGG pathway analysis further indicated that Toll-like receptors and nuclear factor-kappa B-signaling pathways, along with the phosphatidylinositol signaling system, were closely associated with this absorption process. Altogether, the findings of this study demonstrate that QIGLF may alter the expression or subcellular localization of tight junction proteins through these signaling pathways, thereby modulating intestinal tight junction permeability and facilitating its paracellular absorption.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1021/acs.jafc.5c08327
Junhua Di, Yuyang Hu, Yucai He, Cuiluan Ma
Lignocellulose, as the most abundant renewable biomass, offers a sustainable source of valuable chemicals. This study explores the biosynthesis of vanillylamine, a versatile lignin derivative used in producing furans, food additives, and pharmaceuticals, from biobased aldehydes under mild conditions. A transaminase (3FCR-3M) from Ruegeria sp. TM1040 was expressed in E. coli and applied in ethylene glycol-water medium (ethylene glycol, 20 vol%). Under optimized conditions (35 °C, pH 7.5), using L-alanine (L-Ala:vanillin = 5:1, mol:mol) as the amine donor, the whole-cell biocatalyst effectively converted various aromatic and furan aldehydes (50 mM), including vanillin, isovanillin, anisaldehyde, furfural, and HMF, into corresponding amines. Yields ranged from 52.8% to 98.4%. This work demonstrates a green and efficient bioamination strategy for producing vanillylamine and its derivatives from renewable biomass.
{"title":"Biomanufacturing Biobased Organic Amines from Biomass-Derived Aldehydes Through the Amination with Transaminase 3FCR-3M","authors":"Junhua Di, Yuyang Hu, Yucai He, Cuiluan Ma","doi":"10.1021/acs.jafc.5c08327","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08327","url":null,"abstract":"Lignocellulose, as the most abundant renewable biomass, offers a sustainable source of valuable chemicals. This study explores the biosynthesis of vanillylamine, a versatile lignin derivative used in producing furans, food additives, and pharmaceuticals, from biobased aldehydes under mild conditions. A transaminase (3FCR-3M) from <i>Ruegeria</i> sp. TM1040 was expressed in <i>E. coli</i> and applied in ethylene glycol-water medium (ethylene glycol, 20 vol%). Under optimized conditions (35 °C, pH 7.5), using <i>L</i>-alanine (<i>L</i>-Ala:vanillin = 5:1, mol:mol) as the amine donor, the whole-cell biocatalyst effectively converted various aromatic and furan aldehydes (50 mM), including vanillin, isovanillin, anisaldehyde, furfural, and HMF, into corresponding amines. Yields ranged from 52.8% to 98.4%. This work demonstrates a green and efficient bioamination strategy for producing vanillylamine and its derivatives from renewable biomass.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"40 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1021/acs.jafc.5c15111
Xuguo Duan, Jinbo Zhang, Yang Zhao, Qianqian Liu, Yucheng Ding
Myo-inositol, a high-value cyclic polyol, is increasingly sought by pharmaceutical, food, feed, and cosmetic industries. This review systematically surveys the latest biotechnological advances poised to replace traditional, high-pollution methods. First, multienzyme cascades that convert renewable carbohydrates─starch, glucose, xylose, cellulose, sucrose─are reviewed, highlighting immobilized reactors, porous microspheres, biomimetic mineralized capsules, and biofilm systems that boost stability, reusability, and space-time yields. Second, microbial cell-factory strategies are examined, covering chassis benchmarking (Escherichia coli, Pichia pastoris, Kluyveromyces marxianus, cyanobacteria), carbon-flux redirection, glucose-glycerol synergistic feeding, and dynamic regulatory circuits. A unified analysis pinpoints recurrent bottlenecks─cofactor imbalance, enzyme thermostability gaps, narrow substrate spectra, product inhibition, and downstream complexity─and distills the targeted fixes discussed in the field, from cofactor regeneration circuits to modular process design. By integrating cutting-edge research with industrial techno-economic indicators, this review offers a comprehensive roadmap for sustainable, cost-competitive myo-inositol biomanufacturing and guides future research toward greener production systems.
{"title":"Innovative Biotechnological Strategies for Sustainable Myo-Inositol Production and Application","authors":"Xuguo Duan, Jinbo Zhang, Yang Zhao, Qianqian Liu, Yucheng Ding","doi":"10.1021/acs.jafc.5c15111","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c15111","url":null,"abstract":"<i>Myo</i>-inositol, a high-value cyclic polyol, is increasingly sought by pharmaceutical, food, feed, and cosmetic industries. This review systematically surveys the latest biotechnological advances poised to replace traditional, high-pollution methods. First, multienzyme cascades that convert renewable carbohydrates─starch, glucose, xylose, cellulose, sucrose─are reviewed, highlighting immobilized reactors, porous microspheres, biomimetic mineralized capsules, and biofilm systems that boost stability, reusability, and space-time yields. Second, microbial cell-factory strategies are examined, covering chassis benchmarking (<i>Escherichia coli</i>, <i>Pichia pastoris</i>, <i>Kluyveromyces marxianus</i>, <i>cyanobacteria</i>), carbon-flux redirection, glucose-glycerol synergistic feeding, and dynamic regulatory circuits. A unified analysis pinpoints recurrent bottlenecks─cofactor imbalance, enzyme thermostability gaps, narrow substrate spectra, product inhibition, and downstream complexity─and distills the targeted fixes discussed in the field, from cofactor regeneration circuits to modular process design. By integrating cutting-edge research with industrial techno-economic indicators, this review offers a comprehensive roadmap for sustainable, cost-competitive <i>myo</i>-inositol biomanufacturing and guides future research toward greener production systems.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"94 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1021/acs.jafc.5c15391
Tao Zhang, Yu Chen, Weilin Chen, Huayun Chen, Yan Zhang, Jiahao Yan, Yueli Zhou, Genxi Zhang
Understanding chicken skeletal muscle development is crucial for meat production, yet its cellular and molecular mechanisms are not fully understood. To address this, we built a single-nucleus RNA sequencing atlas of chicken skeletal muscle across key developmental stages (E4-P30) using 111,328 nuclei. We identified 22 cell populations, including nine myogenic ones, revealing extensive heterogeneity and transcriptional diversity. Myonuclei were classified into three fiber-type-associated subpopulations: FGF13+ type IIA fast-twitch, MYH1F+ type IIB fast-twitch, and MYH7B+ type I slow-twitch. Pseudotime analysis reconstructed the developmental trajectory from myogenic progenitors to mature myofibers and highlighted RBM24 as a pivotal regulator. Functional validation confirmed that RBM24 promotes myoblast differentiation while inhibiting proliferation. We further identified putative fiber-type-specific regulators (e.g., TBX15 for IIA, TNNT3 for IIB, and FGFRL1 for I). This study delineates myogenic lineage development and identifies key regulatory genes, offering valuable insights into chicken skeletal muscle biology.
{"title":"Single-Nucleus RNA Sequencing Reveals Cellular Heterogeneity and Trajectories of Lineage Differentiation during Chicken Skeletal Muscle Development","authors":"Tao Zhang, Yu Chen, Weilin Chen, Huayun Chen, Yan Zhang, Jiahao Yan, Yueli Zhou, Genxi Zhang","doi":"10.1021/acs.jafc.5c15391","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c15391","url":null,"abstract":"Understanding chicken skeletal muscle development is crucial for meat production, yet its cellular and molecular mechanisms are not fully understood. To address this, we built a single-nucleus RNA sequencing atlas of chicken skeletal muscle across key developmental stages (E4-P30) using 111,328 nuclei. We identified 22 cell populations, including nine myogenic ones, revealing extensive heterogeneity and transcriptional diversity. Myonuclei were classified into three fiber-type-associated subpopulations: <i>FGF13</i><sup>+</sup> type IIA fast-twitch, <i>MYH1F</i><sup>+</sup> type IIB fast-twitch, and <i>MYH7B</i><sup>+</sup> type I slow-twitch. Pseudotime analysis reconstructed the developmental trajectory from myogenic progenitors to mature myofibers and highlighted RBM24 as a pivotal regulator. Functional validation confirmed that RBM24 promotes myoblast differentiation while inhibiting proliferation. We further identified putative fiber-type-specific regulators (e.g., <i>TBX15</i> for IIA, <i>TNNT3</i> for IIB, and <i>FGFRL1</i> for I). This study delineates myogenic lineage development and identifies key regulatory genes, offering valuable insights into chicken skeletal muscle biology.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"216 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Research on iron (Fe)-mediated plant mitigation of cadmium (Cd) stress has achieved significant progress. This review elucidates the dual role of Fe as both a “shield and spear” from a Spatial perspective. Initially, exogenous Fe acts as a rapid “shield” by immobilizing Cd, recruiting beneficial microorganisms, and forming iron plaque in the rhizosphere to inhibit Cd uptake, simultaneously initiating early defense by upregulating Fe uptake systems. Subsequently, Fe shifts to a “spear” function, enhancing long-term plant tolerance by inhibiting Cd transport at cellular levels, repairing photosystem damage, and strengthening antioxidant defenses at molecular levels. These mechanisms provide crucial insights for developing Fe-based agronomic strategies. However, the dose–response toxicity and the trade-off between defense and growth limit field practical application. To address this, we integrate the physicochemical properties of Fe-based materials with agronomic management practices to propose application guidelines, advancing sustainable agricultural development.
{"title":"Iron-Mediated Spatial Cadmium Resistance Mechanisms: Dual Shield and Spear Strategies in Soil–Plant Systems","authors":"Jieting Wu,Ziyi Lian,Yixuan Zhang,Lei Zhao,Yuxin Li,Haoran Yin,Xiaofan Fu,Jing Shang,Chengbin Xu,Runjie Zhang,Fengshuo Yang,Fang Ma","doi":"10.1021/acs.jafc.5c11133","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c11133","url":null,"abstract":"Research on iron (Fe)-mediated plant mitigation of cadmium (Cd) stress has achieved significant progress. This review elucidates the dual role of Fe as both a “shield and spear” from a Spatial perspective. Initially, exogenous Fe acts as a rapid “shield” by immobilizing Cd, recruiting beneficial microorganisms, and forming iron plaque in the rhizosphere to inhibit Cd uptake, simultaneously initiating early defense by upregulating Fe uptake systems. Subsequently, Fe shifts to a “spear” function, enhancing long-term plant tolerance by inhibiting Cd transport at cellular levels, repairing photosystem damage, and strengthening antioxidant defenses at molecular levels. These mechanisms provide crucial insights for developing Fe-based agronomic strategies. However, the dose–response toxicity and the trade-off between defense and growth limit field practical application. To address this, we integrate the physicochemical properties of Fe-based materials with agronomic management practices to propose application guidelines, advancing sustainable agricultural development.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"106 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Parvalbumin (PV) is the major allergen in grass carp (Ctenopharyngodon idellus). Recombinant parvalbumins in Escherichia coli (EcPV) and Pichia pastoris (PpPV) were successfully expressed, with respective molecular weights of 14 kDa and 16 kDa. Mass spectrometry confirmed the identity of the proteins, while ultraviolet spectroscopy, circular dichroism spectroscopy, and fluorescence spectroscopy were used for structural validation. Physicochemical properties were further evaluated through digestibility assays, sIgE inhibition ELISA, Western blotting, and RBL-2H3 cell degranulation experiments. The results indicated that the recombinant proteins exhibited a high degree of amino acid sequence similarity with the natural protein (NaPV) and shared core immunodominant epitopes, while demonstrating higher IgE-binding efficiency compared to the natural protein. In contrast to EcPV, PpPV showed greater resistance to pepsin digestion, displayed differences in secondary structure, and exhibited altered conformational epitopes. Therefore, the degranulation efficiency of RBL-2H3 cells induced by PpPV was lower than that induced by EcPV and NaPV.
{"title":"Comparison of Recombinant Ctenopharyngodon idella Allergen Parvalbumin, Produced in Pichia pastoris and Escherichia coli: From Structure to Functional Properties","authors":"Yuwei Feng, Jiasen Zhang, Hongbing Chen, Ping Tong, Anshu Yang, Yingxue Zhang, Yanhai Xie","doi":"10.1021/acs.jafc.5c14127","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c14127","url":null,"abstract":"Parvalbumin (PV) is the major allergen in grass carp (<i>Ctenopharyngodon idellus</i>). Recombinant parvalbumins in <i>Escherichia coli</i> (<i>Ec</i>PV) and <i>Pichia pastoris</i> (<i>Pp</i>PV) were successfully expressed, with respective molecular weights of 14 kDa and 16 kDa. Mass spectrometry confirmed the identity of the proteins, while ultraviolet spectroscopy, circular dichroism spectroscopy, and fluorescence spectroscopy were used for structural validation. Physicochemical properties were further evaluated through digestibility assays, sIgE inhibition ELISA, Western blotting, and RBL-2H3 cell degranulation experiments. The results indicated that the recombinant proteins exhibited a high degree of amino acid sequence similarity with the natural protein (<i>Na</i>PV) and shared core immunodominant epitopes, while demonstrating higher IgE-binding efficiency compared to the natural protein. In contrast to <i>Ec</i>PV, <i>Pp</i>PV showed greater resistance to pepsin digestion, displayed differences in secondary structure, and exhibited altered conformational epitopes. Therefore, the degranulation efficiency of RBL-2H3 cells induced by <i>Pp</i>PV was lower than that induced by <i>Ec</i>PV and <i>Na</i>PV.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"5 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1021/acs.jafc.5c07569
Milena Moreira Vacilotto,Vanessa de Oliveira Arnoldi Pellegrini,Evandro Aresde de Araujo,Marcelo V. Liberato,Igor Polikarpov
Transformation of agro-industrial products into value-added products, such as prebiotic oligosaccharides, is a key element of the emerging bioeconomy. Here, we characterized a new GH30_8 glucuronoxylanase from Bacillus pumilus (BpXyn30_8A) for its potential in producing xylooligosaccharides (XOS). BpXyn30_8A showed tolerance to ethanol and NaCl and released both linear and branched XOS containing MeGlcA at the penultimate nonreducing end residue. Its X-ray structure, determined at 2.16 Å resolution, revealed high similarity to other glucuronoxylanases. Furthermore, BpXyn30_8A achieved higher xylan conversion yields from corn cob and Eucalyptus sawdust than Ruminococcus champanellensisRcXyn30A. Finally, fermentation assays showed that Bifidobacterium adolescentis metabolized neutral XOS to acetate and lactate, whereas acidic XOS were poorly utilized. These results highlight the potential of BpXyn30_8A as a valuable enzyme for the green transformation of plant biomass into prebiotic oligosaccharides with promising applications in human and animal nutrition, health, and biotechnology.
{"title":"Enzymatic Production of Prebiotic Xylooligosaccharides Using a Bacillus pumilus GH30_8 Glucuronoxylanase: Structural Basis of Glucuronoxylan Recognition and Hydrolysis","authors":"Milena Moreira Vacilotto,Vanessa de Oliveira Arnoldi Pellegrini,Evandro Aresde de Araujo,Marcelo V. Liberato,Igor Polikarpov","doi":"10.1021/acs.jafc.5c07569","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07569","url":null,"abstract":"Transformation of agro-industrial products into value-added products, such as prebiotic oligosaccharides, is a key element of the emerging bioeconomy. Here, we characterized a new GH30_8 glucuronoxylanase from Bacillus pumilus (BpXyn30_8A) for its potential in producing xylooligosaccharides (XOS). BpXyn30_8A showed tolerance to ethanol and NaCl and released both linear and branched XOS containing MeGlcA at the penultimate nonreducing end residue. Its X-ray structure, determined at 2.16 Å resolution, revealed high similarity to other glucuronoxylanases. Furthermore, BpXyn30_8A achieved higher xylan conversion yields from corn cob and Eucalyptus sawdust than Ruminococcus champanellensisRcXyn30A. Finally, fermentation assays showed that Bifidobacterium adolescentis metabolized neutral XOS to acetate and lactate, whereas acidic XOS were poorly utilized. These results highlight the potential of BpXyn30_8A as a valuable enzyme for the green transformation of plant biomass into prebiotic oligosaccharides with promising applications in human and animal nutrition, health, and biotechnology.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"91 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1021/acs.jafc.5c11047
Yulu Lao, Junlin Zhang, Tiantian Wang, Jie Xiao, Yong Cao, Hang Xiao, Xiaojuan Liu
Astaxanthin exists as multiple stereoisomers owing to chiral carbons, with (3S,3′S) (S) and (3R,3′R) (R) as the major natural enantiomer. The differential bioactivity of these isomers implies potential stereoselective absorption. However, whether cluster-determinant 36 (CD36), a key transporter of astaxanthin, exhibits such stereospecificity remains unclear. Herein, molecular docking and alanine scanning mutagenesis revealed a higher affinity (ΔΔG = −1.39 kcal/mol) of (S)-astaxanthin for CD36, attributed to a unique hydrogen bond with ASN53 and critical interactions with PHE300, LEU328, and PHE430. Subsequent CD36-overexpressing cells showed 14.44% higher (S)-enantiomer uptake than (R)-counterpart (P < 0.05). In situ model further revealed superior absorption rate and permeability for (S)-astaxanthin. Critically, the CD36-specific inhibitor sulfo-N-succinimidyl oleate considerably inhibited (S)-astaxanthin absorption in the duodenum and jejunum by 83.78 and 84.01%, respectively, which were notably higher than the inhibitions of (R)-form (49.16 and 36.24%, P < 0.01). This work provides the first evidence that CD36 mediates stereospecific (S)-astaxanthin transport, advancing chiral carotenoid research.
{"title":"Intestinal Absorption of Dietary Astaxanthin Mediated by Cluster-Determinant 36 (CD36) Presents Stereoisomeric Selectivity In Vitro and In Vivo","authors":"Yulu Lao, Junlin Zhang, Tiantian Wang, Jie Xiao, Yong Cao, Hang Xiao, Xiaojuan Liu","doi":"10.1021/acs.jafc.5c11047","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c11047","url":null,"abstract":"Astaxanthin exists as multiple stereoisomers owing to chiral carbons, with (3<i>S</i>,3′<i>S</i>) (<i>S</i>) and (3<i>R</i>,3′<i>R</i>) (<i>R</i>) as the major natural enantiomer. The differential bioactivity of these isomers implies potential stereoselective absorption. However, whether cluster-determinant 36 (CD36), a key transporter of astaxanthin, exhibits such stereospecificity remains unclear. Herein, molecular docking and alanine scanning mutagenesis revealed a higher affinity (ΔΔ<i>G</i> = −1.39 kcal/mol) of (<i>S</i>)-astaxanthin for CD36, attributed to a unique hydrogen bond with ASN53 and critical interactions with PHE300, LEU328, and PHE430. Subsequent CD36-overexpressing cells showed 14.44% higher (<i>S</i>)-enantiomer uptake than (<i>R</i>)-counterpart (<i>P</i> < 0.05). <i>In situ</i> model further revealed superior absorption rate and permeability for (<i>S</i>)-astaxanthin. Critically, the CD36-specific inhibitor sulfo-<i>N</i>-succinimidyl oleate considerably inhibited (<i>S</i>)-astaxanthin absorption in the duodenum and jejunum by 83.78 and 84.01%, respectively, which were notably higher than the inhibitions of (<i>R</i>)-form (49.16 and 36.24%, <i>P</i> < 0.01). This work provides the first evidence that CD36 mediates stereospecific (<i>S</i>)-astaxanthin transport, advancing chiral carotenoid research.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"31 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1021/acs.jafc.5c15231
Jirui Wei, Xiaozheng He, Tingting Shi, Xiayu Jin, Jun Huang, Qingyao Du, Junda Huang, Tao Li, Xunjie Xie, Yuxin Huang, Chengtao Nie, Yiqi Fan, Xixiao Yang, Luyao Song, Waijiao Tang
Epidemiological studies indicate that Metabolic Associated Fatty Liver Disease (MAFLD) has become the most prevalent chronic liver disease globally. Identifying bioactive molecules from natural plants that can prevent and ameliorate MAFLD is an effective approach to discovering new MAFLD therapeutics. Liupao tea possesses antioxidant and metabolic regulatory properties. This study aimed to identify the active components of Liupao tea polyphenol extract (PLE), and its potential targets and mechanisms of action in the prevention of MAFLD. We performed experiments on MAFLD mice to establish the prevention efficacy of PLE against MAFLD and determine its optimal dosage. Furthermore, network pharmacology, molecular docking, and SPR were performed to predict that PLE’s targets for preventing MAFLD concentrate on the hepatic EGFR target. Knockout of EGFR in hepatocytes attenuated the effects of PLE. These findings indicate that PLE inhibits lipid accumulation by downregulating the EGFR/pEGFR-AKT/pAKT-SREBP-1-ACC1 pathway through binding to the EGFR receptor, thereby preventing MAFLD.
{"title":"Network Pharmacology-Based Investigation of the Mechanism of Liupao Tea Polyphenol Extract in Preventing MAFLD via the Hepatic EGFR–AKT Pathway","authors":"Jirui Wei, Xiaozheng He, Tingting Shi, Xiayu Jin, Jun Huang, Qingyao Du, Junda Huang, Tao Li, Xunjie Xie, Yuxin Huang, Chengtao Nie, Yiqi Fan, Xixiao Yang, Luyao Song, Waijiao Tang","doi":"10.1021/acs.jafc.5c15231","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c15231","url":null,"abstract":"Epidemiological studies indicate that Metabolic Associated Fatty Liver Disease (MAFLD) has become the most prevalent chronic liver disease globally. Identifying bioactive molecules from natural plants that can prevent and ameliorate MAFLD is an effective approach to discovering new MAFLD therapeutics. Liupao tea possesses antioxidant and metabolic regulatory properties. This study aimed to identify the active components of Liupao tea polyphenol extract (PLE), and its potential targets and mechanisms of action in the prevention of MAFLD. We performed experiments on MAFLD mice to establish the prevention efficacy of PLE against MAFLD and determine its optimal dosage. Furthermore, network pharmacology, molecular docking, and SPR were performed to predict that PLE’s targets for preventing MAFLD concentrate on the hepatic EGFR target. Knockout of EGFR in hepatocytes attenuated the effects of PLE. These findings indicate that PLE inhibits lipid accumulation by downregulating the EGFR/pEGFR-AKT/pAKT-SREBP-1-ACC1 pathway through binding to the EGFR receptor, thereby preventing MAFLD.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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